JP2012102068A - Melanism promoter for skin and hair, and external medicine for melanism promotion and food or drink product for melanism promotion including the melanism promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel melanism promoter for skin and/or hair, which exhibits an excellent effect on melanism promotion of skin and hair while securing safety in actual use by using a naturally-derived plant, and an external medicine and a drink or food product including the same.SOLUTION: The melanism promoter is composed of an extract obtained by extracting the leaf and stem of Angelica furcijuga Kitagawa belonging to Apiaceae Angelica in a solution of water:1,3 butylene glycol (1:1). This melanism promoter exhibits an excellent effect on melanism promotion of skin and hair.

Description

  The present invention relates to a blackening accelerator that promotes blackening of skin and hair, and an external preparation or food or drink containing the blackening accelerator. More specifically, the present invention relates to a skin or hair blackening promoter, an external preparation, and an invention for foods and drinks containing Hyugatoki (scientific name Angelica furcijuga Kitagawa, hereinafter simply referred to as “Hyugatoki”) as an active ingredient.

Hyugatouki is a perennial plant that belongs to the genus Shisiudo, a celery family, and grows in the hills and mountains of southern Kyushu.
In recent years, the physical activity of its efficacy has attracted attention. For example, it is used for appetite enhancement, fatigue recovery, normalization of metabolic function (see Patent Document 1 etc.), antihepatitis agent, anticancer agent (Patent Document 2). , Patent Document 3, etc.) have been proposed.

Recently, attention has also been focused on the whitening effect by the extract from Toki, that is, the action of suppressing the production of melanin.
Melanin pigments that determine the color of hair and skin are biosynthesized from tyrosine at melanosomes in melanocytes (melanin synthesizing cells). When the melanin pigment increases due to the influence of external factors such as ultraviolet rays and dryness, and internal factors such as aging and stress, it causes “skin dullness” that does not make the skin color dull and loses its gloss and transparency. Many women list dull skin as a cosmetic problem.
In order to improve such problems, for example, Patent Literature 4, Patent Literature 5, Patent Literature 6 and the like disclose that an extract from Tokis suppresses the production of melanin and is useful as a whitening agent. Has been.

On the other hand, when the amount of melanin produced by melanocytes decreases, it becomes a direct cause of gray hair, and it becomes difficult for those who want to darken the skin to make it black as expected.
It is considered that gray hair is associated with a decrease in melanocytes and melanosomes due to aging and stress, or a decrease in the amount and activity of tyrosinase due to abnormalities in these cells and organs, and inhibition of melanosome transport.
In the case of Japanese, black or brownish hair is generally preferred, but many gray hairs appear as a result of aging. This is due to a marked decrease in melanin due to a decrease in the number and activity of melanocytes present in the hair roots, etc., and there is a tendency to be shunned cosmetically and psychologically.

With regard to anti-whitening agents, there are a wide variety of products obtained by screening for effective substances with reference to the mechanism of white hair development and the mechanism of melanin pigment formation, or by examining the white hair prevention action of various substances at random. The use of various ingredients has been proposed.
For example, Patent Document 7 discloses that extracts such as figs and mulberries are useful for darkening skin and hair, and Patent Document 8 discloses extracts of Akinonogeshi.

In addition, when human skin is exposed to ultraviolet rays for a long time, erythema, edema, urticaria, liver spots (spots), sparrow eggs (sobacas) and skin aging (skin atrophy, wrinkles, dry skin symptoms, skin abnormalities) Such as keratinization), senile pigment spots, melanosis, and the risk of skin cancer are known to adversely affect health in the short and long term. Caucasians and others are prone to sunlight skin cancer and are at increased risk of melanoma (skin cancer). Long-term exposure to sunlight, even for colored people, increases the risk of skin cancer and accelerated skin aging.
On the other hand, in the skin, melanin has a function of protecting the skin from the harmful effects of ultraviolet rays, and is produced when irradiated with ultraviolet rays, and greatly affects the browning of the skin.

  Some young people tend to like tanned skin tones. Many of these young people use a means of artificially producing melanin by irradiating a limited range (suntan region) of natural light or a specially designed ultraviolet light source. However, this action may adversely affect the health of the skin as described above, and there is a need for a natural plant-derived skin blackening agent as described above.

  As described above, as far as the present inventors know, it has not been found that Hyugatouki is useful for promoting the darkening of hair and skin.

JP-A-8-256724 JP-A-11-310536 WO 00/27224 JP 2003-160463 A JP 2004-35440 A JP 2004-91351 A JP 2002-47130 A JP 2004-345959 A

  The present invention has been made in view of such conventional circumstances, and the object of the present invention is to provide a novel skin that ensures safety in actual use and exhibits excellent skin and hair blackening accelerator effects. Or it is providing the blackening promoter of hair, an external preparation, and food-drinks.

  In order to solve the above-mentioned problems, the present inventors have conducted intensive studies, and as a result, for the first time, the extract of Hyugatouki has an excellent skin and hair blackening promoting effect, and the present invention has been completed. It was.

  That is, the present invention is a skin and / or hair blackening promoter characterized by comprising an extract of Hyugatouki as an active ingredient.

  As the extract, it is preferable to use an extract of the leaves and / or stems of Hyugatouki.

  Further, the present invention is an external preparation for promoting blackening of skin and / or hair, characterized by containing the blackening promoter.

  Moreover, this invention is the food / beverage products for blackening promotion of the skin and / or hair characterized by including the said blackening promoter.

  In the external preparation for promoting blackening, the lower limit of the content of the extract is preferably 0.05% by weight, and more preferably 0.1% by weight. In the said foodstuff for blackening promotion, it is preferable that the lower limit of content of the said extract is 0.1 weight%, and it is more preferable that it is 0.15 weight%. The upper limit of the content of the extract is not necessarily limited, but the upper limit is preferably 50% by weight and more preferably 20% by weight.

  As described above, the present invention is a novel skin and hair blackening promoter, external preparation, food and drink comprising an extract of Hyugatouki, which is a naturally derived plant, as an active ingredient, and is safe for practical use. And has an excellent effect on promoting the blackening of skin and hair.

The graph which shows the growth rate of the melanin pigment | dye in a cell culture test. The graph which shows the growth rate of the melanin pigment | dye in an application | coating test. The graph which shows the growth rate of the melanin pigment | dye in an administration test.

Hereinafter, the present invention will be described in further detail.
(Blackening accelerator)
In the present invention, as described above, the extract of Hyugatouki is used as an active ingredient.
In obtaining the extract of Hyugatouki, it is conceivable to arbitrarily use the ground part such as the leaf part, the stem part, the flower part, the seed, the root part, or the whole plant as it is, but the leaf part, the stem part, etc. It is more preferable to use the above-ground part. Moreover, the raw ginger can be either raw or dried, and is cut or pulverized as necessary, and an extraction operation is performed with an appropriate extraction solvent.

  As the extraction solvent, any solvent that is usually used for plant extraction, such as a polar solvent, a nonpolar solvent, or a mixture thereof, can be used.

  Specifically, as a polar solvent, water or hot water, alcohols (methanol, ethanol, isopropanol, n-propanol, isobutanol, n-hexanol, methylamyl alcohol 2-ethylbutanol, n-octanol, etc.), polyvalent Alcohol or its derivatives (ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol, etc.) , Ketones (acetone, methyl acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl-n-propyl ketone, etc.), esters (ethyl acetate, acetic acid, etc.) An isopropyl, etc.), can be exemplified ethers (ethyl ether, isopropyl ether, n- butyl ether), one or two or more of them mixed solvent, of the like.

  Nonpolar solvents include aliphatic hydrocarbons (petroleum ether, n-hexane, n-pentane, n-butane, n-octane, cyclohexane, etc.), carbon tetrachloride, chloroform, dichloromethane, trichloroethylene, benzene, toluene, etc. One of these, or a mixed solvent of two or more of these can be mentioned. A mixed solvent of the polar solvent and the nonpolar solvent described above can also be used.

  The extract of Hyugatouki obtained using such a solvent (hereinafter sometimes referred to as “Hyugatoki extract”) is the skin or hair blackening promoter according to the present invention.

(External preparation for promoting blackening)
Examples of external preparations for promoting blackening according to the present invention include preparations for scalp and hair, and external preparations for skin. In any case, it goes without saying that the active ingredient Hyugatoki extract is contained, but the ingredients usually used in scalp and hair preparations such as hair nourishing agents and skin external preparations are used in the present invention. As long as the effect is not impaired, it can be contained as needed.

  Examples of the usually used components include diluents (water, ethanol, isopropyl alcohol, glycols, etc.), anionic surfactants (alkyl benzene sulfonates, polyoxyalkylene alkyl sulfates, alkyl sulfates, olefins). Sulfonates, alkyl phosphates, polyoxyalkylene alkyl ether phosphates, dialkyl sulfosuccinates and fatty acid salts, etc.), nonionic surfactants (polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyhydric alcohols) Fatty acid partial ester, polyoxyethylene polyhydric alcohol fatty acid partial ester, polyglycerin fatty acid ester, polyoxyethylene hydrogenated castor oil derivative, fatty acid diethanolamide, etc.), cationic surfactant (No. Grade aliphatic amine salts, alkyltrimethylammonium halides, dialkyldimethylammonium halides and alkyldimethylbenzylammonium halides), amphoteric surfactants (amide betaine type, imidazolinium betaine type and sulfobetaine type surfactants, etc.), high grade Alcohol (cetyl alcohol, stearyl alcohol, behenyl alcohol, etc.), oil (higher fatty acids, solid paraffin, liquid paraffin, silicone oil, polymeric silicone and derivatives thereof, squalane, petrolatum, ester oil, etc.), moisturizer (glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, etc.) and thickeners (methyl cellulose, hydroxyethyl cellulose, etc.). That.

Further, the scalp and scalp hair preparation further contains a hair nourishing component, and can positively impart the hair nourishing effect simultaneously with the anti-white hair effect.
Examples of hair nourishing ingredients include plant extracts (such as assembly extract, carrot extract, etc.), vitamins (vitamin B6, vitamin E and derivatives thereof, vitamins such as biotin), pantothenic acid and Derivatives, glycyrrhizic acid and its derivatives, nicotinic acid esters (benzyl nicotinate, etc.), amino acids (serine, methionine, arginine, etc.), cephalanthin, capronium chloride, minoxidil, nicorandil, acetylcholine derivatives, cyclosporines, female hormone agents (Such as estradiol), antibacterial agents (such as hinokitiol, hexachlorophene, benzalkonium chloride, cetylpyridinium chloride, undecylenic acid, trichlorocarbanilide, and bithionol), cooling agents (such as menthol), salis Le acid, zinc and derivatives thereof, active substances (lactic acid and its alkyl esters, etc.), organic acids (such as citric acid), and the like.

The external preparation for promoting blackening takes any form as long as it has a property that can be applied to or penetrates the skin, scalp, and hair, such as a liquid agent, an emulsifier, a gelling agent, an ointment, an aerosol agent, and a mousse agent. be able to. Specifically, it takes the form of products called emollient lotion, emollient cream, moisture gel, lotion, hair tonic, hair liquid, scalp treatment, hair cream, aerosol mousse, aerosol spray, hair gel, spray mousse, etc. Can do.
It is conceivable that the content of the Hyugatouki extract in the external preparation for promoting blackening is 0.01 to 100% by weight in the total amount of the external preparation. The lower limit of the preferred content is 0.05% by weight, and if it is less than 0.05% by weight, no significant effect on the promotion of blackening is observed. A more preferable lower limit of the content is 0.1% by weight, and when the content is 0.1% by weight or more, a further remarkable blackening promoting effect appears. In addition, it cannot be overemphasized that other components as mentioned above contain a suitable quantity as needed.

(Black and white food and drink)
The food and drink for promoting blackening according to the present invention is less likely to cause human health hazards and is ingested by oral or gastrointestinal administration in daily life, for example, general food and drink, health food, Includes health drinks, health functional foods, health functional drinks, nutritional supplements, nutritional supplements, quasi-drugs, and pharmaceuticals.
Moreover, the above-mentioned Hyugatouki extract may be contained in an appropriate amount, or the Hyugatouki extract itself may be used.

  The form of the food for promoting blackening is not particularly limited, and can be appropriately selected according to the purpose. For example, beverages (soft drinks, carbonated drinks, nutrient drinks, fruit drinks, lactic acid drinks, etc.), frozen desserts (ice cream, ice sherbet, shaved ice, etc.), noodles (soba, udon, harusame, Chinese noodles, instant noodles, etc.), dumplings Skin, shumai skin, confectionery (strawberry, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread, etc.), seafood (crab, salmon, clams, tuna, sardines, Shrimp, skipjack, mackerel, whale, oyster, saury, squid, red scallop, scallop, abalone, sea urchin, salmon roe, tocobushi, etc., fishery and livestock processed foods (kamaboko, ham, sausage, etc.), dairy products (processed milk, fermented milk) Etc.), fats and oils and processed foods (dairy products, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dress ), Seasonings (sauce, sauce, etc.), retort pouch foods (curry, stew, oyakodon, rice bowl, miscellaneous rice, Chinese rice bowl, bonito, tempura, eel rice, hayashi rice, oden, mabodorf, beef bowl, meat sauce, Egg soup, omelet rice, dumplings, shumai, hamburger, meatballs, etc.), various other forms of health foods, health drinks, nutritional supplements, nutritional supplements, and pharmaceuticals such as tablets, capsules, drinks, troches, Various aspects such as quasi-drugs can be exemplified.

Since the content of the Hyugatouki extract in the food for promoting blackening differs depending on the type of food or drink to be targeted, it cannot always be specified unconditionally.
For example, when it aims at containing in the range which does not impair the original taste of food / beverage products, it is possible to make content of a Hyugatouki extract 0.001-50 weight%. The lower limit of the preferred content is 0.1% by weight, and if it is less than 0.1% by weight, no significant effect on the promotion of blackening is observed. A more preferable lower limit of the content is 0.15% by weight, and when the content is 0.15% by weight or more, an even more significant blackening promoting effect appears.
Moreover, when it is set as the form of a granule, a tablet, a capsule, a drink, etc., it is possible to make content of a Hyugatouki extract into 0.01 to 100 weight%, and it is set as 5 to 100 weight%. More preferred.

  Next, test examples and examples using samples will be described, but the present invention is not limited to these samples, test examples, and examples.

(Test Example 1: Comparison of melanin growth rate by cell culture)
(Preparation of Hyugatouki extract)
First, 50 g of dried Hyugatouki leaves and stems were extracted with 1 L of water: 1,3 butylene glycol (1: 1) solution at 80 to 100 ° C., and about 500 mL of Hyugatouki was extracted. I got a thing.

(Cell culture and cell number adjustment)
Next, mouse melanoma cells (B16 cells) are seeded in a petri dish for cell culture so that the number of cells is 1 × 10 ⁵ per 1 mL, and the cells are cultured in a culture vessel under conditions of 37 ° C. and 5% CO _2. Evening culture (preculture) was performed.
Here, the above Hyugatouki extract was added so that the contents were 0.05% by weight (Sample 1), 0.10% by weight (Sample 2), and 0.20% by weight (Sample 3), respectively. Culture was performed for 72 hours under the same conditions (main culture).
After incubation, trypsinize each sample to detach the cells, collect the cells by centrifugation, and count the number of cells for each content so that the number of cells is 1 × 10 ⁶ per mL. ,It was adjusted.
On the other hand, as a control group (Comparative Example 1), prepared by adding 1,3-butylene glycol to a content of 0.20% by weight under the same conditions as described above, counting the number of cells in the same manner as described above, It adjusted so that it might become the same number.

(Evaluation of growth rate of melanin pigment)
Next, the growth rate of melanin pigment (ratio of each sample with respect to Comparative Example 1) is shown for each of the mouse melanoma cells in Samples 1 to 3 and Comparative Example 1 in which the number of cells was adjusted as described above.
After adjusting the number of cells, with respect to Samples 1 to 3 and Comparative Example 1, the absorbance of the melanin pigment in the cell lysate was measured with a microplate reader (spectrophotometer) at a wavelength of 405 nm, which is the absorption band of the melanin pigment. did. The number of measurements was the average of 7 cultures for each sample. As the biosynthesis of melanin pigment is promoted and the degree of proliferation increases, the absorbance increases. Therefore, for Samples 1 to 3, the relative value when Comparative Example 1 is 100% is defined as “growth rate of melanin pigment” and Table 1 As shown in FIG. As shown in the table, Sample 2 and Sample 3 were significantly different from Comparative Example 1 at the significance level p = 5%. It was also confirmed that the growth rate increased as the concentration of Hyugatouki extract increased.

(Evaluation by color tone)
For Samples 1 to 3 and Comparative Example 1, the cells with the cell number adjusted as described above were dissolved in a 1 mol / L NaOH solution, and the change in color tone was visually observed. When it was dark, it was evaluated as +, when it was darker, ++, and when it was darker, it was evaluated as +++. The results are shown in Table 2. As a result, it was confirmed that the color tone became deeper as the concentration of Hyugatouki extract increased.

(Test Example 2: Comparison of proliferation rate of melanin pigment by application)
(Preparation of Hyugatouki extract)
First, as in Test Example 1, 50 g of dried Hyugatouki leaves and stems were boiled at 80 to 100 ° C. in 1 L of a water: 1,3 butylene glycol (1: 1) solution, and an extraction operation was performed. About 500 mL of Hyugatouki extract was obtained. This Hyugatouki extract was added to 1% xanthan gum gel so that the contents would be 0.10 wt% (Sample 4), 1.00 wt% (Sample 5), 10.0 wt% (Sample 6), respectively. Thus, the gel samples 4, 5, and 6 were obtained.

(Repeated application)
Next, the above samples 4 to 6 were repeatedly applied to the back of a mouse (C57BL / 6) from which the back hair was cut and collected for 3 weeks. Three weeks later, the growing back hair was cut and collected.
On the other hand, as Comparative Example 2, xanthan gum 100% gel was used, and the same amount of back hair was cut and collected under the same conditions as described above.

(Evaluation of growth rate of melanin pigment)
Next, the back hair after application in Samples 4 to 6 and Comparative Example 2 was dissolved in a 1 mol / L NaOH solution, and the absorbance of the melanin pigment in the solution was measured with a microplate reader (spectrophotometer). The measurement was performed at a wavelength of 405 nm which is an absorption wavelength band of the dye. The number of measurements was the average of five cultures for each sample. As the biosynthesis of melanin pigment is promoted and the degree of proliferation increases, the absorbance increases. Therefore, with respect to Samples 4 to 6, the relative value when Comparative Example 2 is 100% is expressed as “Melanine pigment growth rate” in Table 3 and As shown in FIG. As shown in the table, Sample 5 and Sample 6 were significantly different from Comparative Example 2 at the significance level p = 5%. It was also confirmed that the growth rate increased as the concentration of Hyugatouki extract increased.

(Evaluation by color tone)
Regarding the back hair before and after application in Sample 6 and Comparative Example 2, when the cells were dissolved with a 1 mol / L NaOH solution, the color tone was visually observed. In Comparative Example 2, no change was observed. In sample 6, it was confirmed that the color tone became dark after application (see Table 4).

(Test Example 3: Comparison of growth rate of melanin by oral administration)
(Preparation of Hyugatouki extract)
First, 30 g of dried Hyugatouki leaves and stems were boiled in hot water at 80 to 100 ° C. to perform an extraction operation to obtain a Hyugatouki extract. Further, this Hyugatouki extract was shaped with lactose so that the contents were 0.10 wt% (Sample 7), 0.15 wt% (Sample 8), and 1.50 wt% (Sample 9), respectively. , Powdered samples 7, 8, and 9 were obtained so that each would be about 20 g.

(Repeated administration)
Next, the sample 7-9 dissolved in water was administered to a mouse (C57BL / 6) from which the back hair was cut and collected for 3 weeks. After 3 weeks, the back hair that had grown again was cut and collected.
On the other hand, as Comparative Example 3, only water was given.

  Next, the dorsal hair after administration in Samples 7 to 9 and Comparative Example 3 was dissolved in a 1 mol / L NaOH solution, and the absorbance of the melanin pigment in the solution was measured with a microplate reader using the absorption wavelength band of the melanin pigment. Measured at a wavelength of 405 nm. The number of measurements was the average of five cultures for each sample. As the biosynthesis of melanin pigment is promoted and the degree of proliferation increases, the absorbance increases. Therefore, for Samples 4 to 6, Table 5 and FIG. 3 shows. As shown in the table, Sample 8 and Sample 9 were significantly different from Comparative Example 3 at the significance level p = 5%. It was also confirmed that the growth rate increased as the concentration of Hyugatouki extract increased.

(Evaluation by color tone)
When the change in color tone of the back hair before and after administration in Sample 9 and Comparative Example 3 dissolved in a 1 mol / L NaOH solution was visually observed, no change was observed in Comparative Example 3, and Sample 9 Then, it was confirmed that the color tone became darker after administration (see Table 6).

  From the above results, it was confirmed that the blackening accelerator, the external preparation for promoting blackening, and the food for food and drink for promoting blackening according to the present invention have a desired blackening promoting effect.

Hereinafter, more detailed examples will be described.
(Example 1: Hair blackening promoting lotion as a hair blackening promoting external preparation)
Hyugatouki extract, polyoxyethylene dioleic acid methyl glucoside (surfactant), L-menthol (penetration enhancer), preservative, fragrance, heated and dissolved at 60 ° C, mixed with purified water and homomixed And the blackening acceleration | stimulation lotion for hair whose content rate of each component is as follows was obtained.
Hyugatouki extract 10% by weight
Polyoxyethylene dioleic acid methyl glucoside 0.2% by weight
L-menthol 0.2% by weight
Preservative 0.2% by weight
Fragrance 0.1% by weight
Purified water balance

(Example 2: Skin blackening promoting cream as an external preparation as a skin blackening promoting external preparation)
Sodium stearate, polyoxyethylene sorbitan monostearate (20EO), behenyl alcohol, self-emulsifying type glyceryl monostearate heated and dissolved at 75 ° C., mixed and treated with a homomixer to make A phase (oil phase), The extract, concentrated glycerin, 1,3-butylene glycol, preservative, and fragrance are heated and dissolved, mixed with purified water and treated with a homomixer to make a B phase (aqueous phase), and these A and B phases are mixed. Thus, a skin darkening promoting cream having the following content ratio of each component was obtained.
(Phase A)
Sodium stearate 5% by weight
3% by weight polyoxyethylene sorbitan monostearate
2% by weight of behenyl alcohol
Self-emulsifying glyceryl monostearate 3% by weight
(Phase B)
Hyugatouki extract 10% by weight
Concentrated glycerin 5% by weight
1,3-butylene glycol 5% by weight
Preservative 0.2% by weight
Fragrance 0.2% by weight
Purified water balance

(Example 3: Skin darkening promoting gel as a skin darkening accelerator)
Carboxyvinyl polymer, concentrated glycerin, and 1,3-butylene glycol are heated and dissolved at 60 to 75 ° C., mixed and homomixed, and treated as a phase A (oil phase). Hyugatouki extract, preservative and flavoring agent are 60 Skin heated and dissolved at ˜75 ° C., mixed with purified water and treated with a homomixer to form B phase (aqueous phase), and further, B phase is added to A phase, and the content ratio of each component is as follows A blackening promoting gel was obtained.
(Phase A)
Carboxy vinyl polymer 1% by weight
Concentrated glycerin 5% by weight
1,3-butylene glycol 5% by weight
(Phase B)
Hyugatouki extract 10% by weight
Preservative 0.2% by weight
Fragrance 0.2% by weight
Purified water balance

(Example 4: Tablet-like dietary supplement as food and drink for promoting blackening of skin and hair)
Each powdered Hyugatouki extract, cellulose powder, and sucrose fatty acid ester heated and dissolved at 60 to 75 ° C. are mixed with lactose (excipient), homomixed and processed into tablets. The tablet-like nutritional supplement whose content rate of each component is as follows was obtained.
Hyugatouki extract 1.5% by weight
Lactose 53.5% by weight
Cellulose powder 44% by weight
Sucrose fatty acid ester 1% by weight

(Example 5: Granular dietary supplement as food and drink for promoting skin and hair blackening)
Each of the powdered Hyugatouki extract, dextrin, and citric acid, heated and dissolved at 60-75 ° C., is mixed with glucose (excipient), homomixed, and then molded into powder form. A granular dietary supplement having the following content ratio was obtained.
Hyugatouki extract 1.5% by weight
Glucose 35% by weight
Dextrin 58.5% by weight
Citric acid 5% by weight

For each of these examples, the same test as in the above test example was conducted, and it was confirmed that the same result as above was obtained.
The embodiments of the present invention have been described with reference to samples, test examples, examples, and the like, but the present invention is not limited to the above-described embodiments, and changes can be made without changing the gist of the invention. It goes without saying that it is possible.

Claims (8)

  A skin and / or hair blackening promoter comprising an extract of Hyugatouki as an active ingredient.   The skin and / or hair blackening promoter according to claim 1, wherein leaves and / or stems of Hyugatouki are used.   An external preparation for promoting blackening of skin and / or hair, comprising the blackening promoter according to claim 1 or 2.   The external preparation for promoting blackening of skin and / or hair according to claim 3, wherein the content of the extract is 0.05% by weight or more.   The external preparation for promoting blackening of skin and / or hair according to claim 3, wherein the content of the extract is 0.1 to 10% by weight.   A food and drink for promoting blackening of skin and / or hair, comprising the blackening promoter according to claim 1 or 2.   The skin and / or hair blackening food or drink according to claim 6, wherein the content of the extract is 0.1% by weight or more.   Content of the said extract is 0.15-1.5 weight%, The food-drinks for skin and / or hair blackening promotion described in Claim 6 characterized by the above-mentioned.

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