JP2012055211A - Method for producing gaba-containing gaba barley, and product of the gaba barley - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a high production technology of GABA, using barley as the material, and especially, for efficiently producing in large quantity, GABA-containing GABA barley and GABA barley bran, and GABA-containing bran supernatant liquid, using barley.SOLUTION: A method for highly producing GABA barley and GABA barley bran highly containing γ-aminobutyric acid, and GABA-containing bran supernatant liquid includes mixing barley grains refined to 5-35% with a barley cleaning machine, and a glutamic acid solution adjusted to pH 3-7 in 0.3-3% concentration, so as to efficiently peel skin because of hard barley skin, and soaking the mixture for one hour to one night (about 15 h) at room temperature, and similarly, soaking it for one hour to one night followed by draining, and leaving the product for one night at room temperature so as to change glutamic acid to γ-aminobutyric acid (GABA) owing to the action of glutamic acid decarboxylase contained in barley.

Description

  The present invention relates to a GABA barley containing GABA, which can easily produce GABA using cereal grains, especially barley, a GABA barley bran, a GABA-containing bran supernatant liquid manufacturing method, and a product thereof.

  GABA is a non-protein amino acid derived from GABA, an acronym for gamma-amino-butyric acid, which is γ-aminobutyric acid, and is not contained in protein. Hereinafter, γ-aminobutyric acid is expressed by GABA or GABA in the drawings.

In patent document 1, it is provided about the processed barley product which contains abundant GABA, and its processing method, even after the barley processing.
The barbecue remaining rate of barley seeds treated with barley with a culling accuracy of 60% is 50% or more compared to unprocessed barley seeds. The barley processing method includes a step of polishing barley seeds without germination.

In patent document 2, in the high GABA containing alcoholic beverage and its manufacturing method, the high GABA regarding the high GABA containing alcoholic beverage is derived from malt (described in claim 3 of JP 2003-250512 A related to Patent Literature 2). Is).

Patent Document 3 discloses a method for producing edible sesame seeds that increases the GABA content, but in grains such as bare wheat, glutinous wheat, millet, millet, millet, germination. There has been no development of ingredients containing a large amount of GABA.

In Patent Document 4, in food materials or foods and methods for producing them, novel food materials or foods enriched with alanine and γ-aminobutyric acid are disclosed, but these include millet, millet, millet, Alanine and γ-aminobutyric acid, as compared to non-germinated cereals that germinate one or more cereals or grains containing cereals selected from the group consisting of pearl barley, buckwheat, amaranthus, quinoa, corn, red beans, sesame and barley Is enriched.

JP 2007-252254 A JP 2003-250512 A JP 2007-330129 A JP 2003-159017 A

  The problem to be solved is to provide a high production technique of GABA using grains, particularly barley, and to use GABA barley containing GABA in large quantities using barley, GABA barley bran and GABA containing bran supernatant liquid. It is a point that cannot be efficiently mass-produced.

  The present invention relates to barley pearl grains refined to 5% or more and 35% or less by a wheat mill, and a glutamic acid solution (L-Glu) adjusted to a pH of 3 to 7 at a concentration between 0.3% and 3%. ), And soaked at room temperature for 1 hour to overnight (about 15 hours). Similarly, after being soaked for 1 hour to overnight, drained and left at room temperature overnight, glutamic acid contained in barley The main feature is that glutamic acid is converted into γ-aminobutyric acid (GABA, GABA) by the action of decarboxylase, and γ-aminobutyric acid is highly produced using barley as a material.

The method for producing a GABA barley having a high GABA content according to the present invention is a method for producing glutamic acid decarboxylase contained in barley by mixing and leaving the barley grains of barley refined from brown barley and a glutamic acid solution adjusted in concentration and pH. In order to use the function, it is easy to produce a large amount of GABA by leaving it for a long time by mixing both the degree of whitening from barley to barley and the glutamic acid solution adjusted in concentration and pH. According to the production method, a GABA barley bran and a GABA-containing bran supernatant are produced using the remaining bran obtained by polishing the barley skin using a barley apparatus. The device for whitening from the brown wheat can be polished using a wheat polishing device or a rice polishing device. The GABA barley grains containing dried GABA and the barley bran and GABA-containing bran supernatant liquid have the advantage of being used as foodstuffs for the production of food containing GABA.

FIG. 1 is a GABA barley manufacturing process diagram showing a method for manufacturing GABA barley products from Nijo barley brown barley as a flowchart. Example 1 FIG. 2 is an explanatory diagram showing the content of GABA contained in 100 g of barley from barley to 35% of whiteness with the horizontal axis of the barley degree of barley. Example 1 FIG. 3 is an explanatory diagram showing in a graph the content of 100% barley with 35% whiteness of GABA produced, with the concentration of glutamic acid on the horizontal axis relative to the concentration of glutamic acid solution. Example 1 FIG. 4 is a graph showing the GABA content generated with the horizontal axis representing the pH adjusted for the glutamic acid solution. Example 1 FIG. 5 is an explanatory diagram suggesting that, in an experiment of bran remaining 35% whitened from Nijo barley, the barley bran and glutamic acid solution were mixed and allowed to stand overnight to produce a GABA content of 27%. (Example 2)

  In the present invention, in order to produce barley grains having a high GABA content and barley bran and GABA-containing bran supernatant, L-glutamic acid, which is a GABA starting material, is added and kept at a room temperature of about 25 degrees Celsius. Therefore, the method of converting to wheat grains and bran was adopted. By this manufacturing method, refined barley, barley bran gabha hatched product and gabba-containing bran supernatant were realized.

  FIG. 1 shows an embodiment of a process of a production method for producing a high-quality product of gabba barley and barley bran from brown wheat according to the method of the present invention.

  Examples will be described with reference to the reference numerals. 50 g of barley 1 is refined by a rice milling device to make barley 2, and after 4 immersed in 100 ml of L-glutamic acid solution adjusted to pH 3 to pH 7 overnight at room temperature, Drained 5 and kept warm 6 nights in a slightly moist condition.

  The glutamic acid-treated wheat grains that had been kept warm were dried 7 at 65 ° C. for 3 hours.

  Sample 8 of dried wheat grains was pulverized with a bean mill.

  To the ground sample 8, 30 ml of 2% (W / V) sulfosalicylic acid was added and vigorously shaken for 10 minutes.

  After centrifugation (1000 × g, 10 minutes), the supernatant was collected as a sample for GABA analysis.

  The amount of GABA in the GABA analysis sample was quantified with an amino acid analyzer (HPLC method).

    The result of measuring the GABA content for the sample of the previous item 0011 is shown in FIG.

  As a result of the preceding item 0017, almost no GABA was produced in the unpolished barley, but a large amount of GABA was produced in the barley refined under 15 to 20% whitening conditions.

  The barley shown in the results of the preceding paragraphs 0017 and 0018 was produced by half to one third even at 30% or 35% having higher whitening degree than the optimum condition of 15%. Since the barley used had a hard skin, the GABA production capacity was maintained even by scouring stronger.

  As shown in the preceding paragraphs 0018 and 0019, it was found that it is essential for barley production to be processed with barley in barley, but when immersed in a glutamic acid solution of any concentration in the treatment shown in the preceding paragraph 0010, It was investigated whether Gabba was produced well. 35% milled barley was soaked in a solution adjusted to pH 4 for 0 to 3% glutamic acid and sodium glutamate solution.

FIG. 3 shows the analysis result of the sample of the preceding item 0020. Good GABA production was confirmed between 0.3% and 3% of glutamic acid and sodium glutamate solution, which are recognized as food additives.
Good GABA production is expected between 0.3% and 3% of other potassium glutamate, magnesium glutamate and calcium glutamate solutions that are recognized as food additives.

  From the analysis results of the GABA content of GABA barley so far, barley containing high concentration GABA can be produced by immersing barley barley having a whiteness of 35% in a glutamic acid solution having a concentration of 1%. In the pH range of the glutamic acid solution, it was investigated whether good GABA production is expected.

FIG. 4 shows the analysis result of the sample of the preceding item 0022. Good GABA production was confirmed by adjusting the pH of the glutamic acid solution from 3 to 7.

  Referring to FIG. 5, a description will be made with reference to FIG. 5. After mixing 35% of barley, 25 g of barley bran, 25 g of barley bran, and a glutamic acid solution (1% concentration, pH 4, 100 ml) are mixed for 9, overnight ( About 15 hours) 10 was observed, and 27% GABA production 11 was observed in the barley bran and bran supernatant (centrifugated supernatant). Centrifugation (1000 × g, 10 minutes) is performed, and as a result, a supernatant (solution) and bran (precipitation) are formed. The experimental procedure and results are shown in FIG. Gabba barley bran products can be produced using the remaining bran from 25% to 35% of barley in a barley apparatus. Moreover, the supernatant liquid after a process can be utilized as a barley bran bran supernatant liquid. Drying GABA barley bran by heat treatment at 65 to 70 ° C. for about 3 hours is an effective storage method for sterilizing miscellaneous bacteria.

  According to the production methods of Example 1 and Example 2, the barley brown barley can be used as a food by producing the GABA barley containing GABA, the GABA barley bran and the GABA containing bran supernatant.

  The food containing the GABA produced by the production method of the present invention is pulverized, mixed in the noodle, udon and pasta production process, or cooked with the GABA barley, and the GABA bran supernatant is added. It can also be applied to various food applications by manufacturing the used food and adding it to other foods such as soft drinks.

1 Barley 2 Barley 3 L-glutamic acid solution 4 Immersion 5 Draining 6 Heat retention 7 Drying 8 Sample 9 Mixing barley bran and glutamic acid solution 10 About placing overnight (about 15 hours)

Claims (12)

  A method for producing GABA barley containing GABA in barley by immersing barley in a glutamic acid solution using barley that has been polished between 5% and 35%.   The barley barley according to claim 1 is a barley barley produced by peeling off the skin of brown barley with a barley apparatus, wherein the barley is produced.   The glutamic acid solution according to claim 1, wherein barley is soaked using sodium glutamate or glutamic acid.   The glutamic acid solution according to claim 3, wherein the glutamic acid solution is a solution having a concentration of between 0.3% and 3%, and is used by adjusting the pH between 3 and 7. Production method.   A method for producing GABA barley containing GABA, wherein barley barley is soaked at a rate of 2 kg per 6 L of the glutamic acid solution according to claim 1 for 1 hour or more at room temperature.   A method for producing GABA barley containing GABA, comprising immersing barley barley in the glutamic acid solution according to claim 5, draining it, and keeping it warm at room temperature. The barley barley product manufactured by the manufacturing method of the barley barley which contains barley with barley by immersing in the glutamic acid solution the barley barley which was refined between 5% and 35% of barley.
  A method for preserving GABA barley, wherein GABA barley containing GABA produced from barley is dipped and stored at 65 ° C to 70 ° C by immersing the barley that has been refined using barley as a material in a glutamic acid solution.   The manufacturing method of the GABA barley bran containing the GABA which manufactures barley bran by mixing a barley bran and a glutamic acid solution and putting it for about 15 hours. A method for producing a GABA-containing bran supernatant containing a GABA that produces barley bran by mixing barley bran and a glutamic acid solution for about 15 hours.
  A GABA barley bran product produced by a method for producing GABA barley bran, which contains GABA produced by mixing barley bran and a glutamic acid solution for about 15 hours. A barley bran supernatant liquid product produced by a method for producing a GABA-containing bran supernatant containing a GABA produced by mixing barley bran and a glutamic acid solution for about 15 hours.

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