JP2012039925A - Lactic acid fermented food material and method for producing thereof - Google Patents

Lactic acid fermented food material and method for producing thereof Download PDF

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JP2012039925A
JP2012039925A JP2010183326A JP2010183326A JP2012039925A JP 2012039925 A JP2012039925 A JP 2012039925A JP 2010183326 A JP2010183326 A JP 2010183326A JP 2010183326 A JP2010183326 A JP 2010183326A JP 2012039925 A JP2012039925 A JP 2012039925A
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lactic acid
food material
fermented food
acid bacteria
pediococcus
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JP5116178B2 (en
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Kotaro Yamagishi
耕太郎 山岸
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SNOW SHOJI KK
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Abstract

PROBLEM TO BE SOLVED: To provide a lactic acid fermented food material of which taste is improved by processing vegetables, for example, into the pureed food material which allows a person to easily ingest to be the fruity taste excellent for habitual eating into a food that can be processed into the food such as ice cream, yogurt, jam or puree, and to provide a method of producing thereof.SOLUTION: There is provided the lactic acid fermented food material in which a vegetable crushed or ground material including ≥8 wt.% of carbohydrate is fermented by the lactic acid bacteria composed of a combination of at least genus Pediococcus and genus Enterococcus.

Description

この発明は乳酸菌発酵食品素材及びその製法に係り、より詳しくは炭水化物を8重量%以上含む野菜類の破砕物、磨砕物を、少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌で発酵させて野菜類をフルーツ風味に処理し例えばピューレ状食品用素材としたうえアイスクリーム、ヨーグルト、ジャム、ピューレなどの食品に加工できる乳酸菌発酵食品素材及びその製法に関する。   The present invention relates to a lactic acid bacteria fermented food material and a method for producing the same, and more specifically, a crushed and ground product of vegetables containing 8% by weight or more of carbohydrates, and a lactic acid bacterium comprising a combination of at least Pediococcus and Enterococcus. The present invention relates to a fermented food material for lactic acid bacteria that can be fermented and processed into fruit flavors to produce a puree food material and processed into foods such as ice cream, yogurt, jam, and puree, and a method for producing the same.

一般に乳酸菌は、発酵乳、乳酸菌飲料、チーズ、漬物等の食品の保存性改善作用、風味改善作用に応用されている。
乳酸菌は、植物由来、動物由来等の種々の菌種が存在する。発酵食品に使用する乳酸菌としては、目的とする食品の風味や味覚により、用いる乳酸菌の種類を変更採用することによって、異なる風味、所望の製品の香味とする良い種類や組み合わせが検討され提案されている。
In general, lactic acid bacteria are applied to preservability improving action and flavor improving action of foods such as fermented milk, lactic acid bacteria beverages, cheese and pickles.
There are various types of lactic acid bacteria such as plants and animals. As lactic acid bacteria used in fermented foods, different types of lactic acid bacteria used depending on the flavor and taste of the target food, and different types of flavors, good types and combinations of desired product flavors have been studied and proposed. Yes.

一方、豆類、イモ類、根茎などの穀物野菜類、根菜類野菜類は加熱が必要であるなど調理に手間がかかる。野菜類の種類によれば匂いが青臭い、あるいは全般に味覚に嗜好が強く好き嫌いが大きいなどの問題があるがこれら一般に野菜類はビタミン類、食物繊維、ミネラルなど多くの栄養素が多く含まれて健康的に常用的に喫食することが望まれている。
そこで野菜類の風味改善に乳酸菌発酵処理をすることが提案されている。
特に、子供や老人に常食として喫食しやすい風味や味覚を持ったフルーツ風味を持った食品形態とすることが望まれている。
On the other hand, beans, potatoes, cereal vegetables such as rhizomes, and root vegetables must be heated. According to the type of vegetables, there is a problem that the smell is blue smell, or the taste is generally strong and the taste is strong and likes and dislikes, but in general these vegetables are rich in many nutrients such as vitamins, dietary fiber, minerals etc. It is desired to eat regularly.
Therefore, it has been proposed to perform lactic acid bacteria fermentation treatment to improve the flavor of vegetables.
In particular, it is desired to have a food form having a flavor and taste that is easy to eat as a regular meal for children and the elderly.

例えば、ラクトバチルス・ブレビス(特公昭58−15109号)は野菜類ミックスジュースの加熱臭の除去と、ジアセチルを産生しない点に重きを置いた飲食品の調製、ラクトバチルス・カゼイ(特開平3−292869号)は酢酸様のにおいを生じさせない発酵食品の調製、ラクトバチルス・ブルガリクスとストレプトコッカス・サーモフィルスとの組み合わせにより(特開昭60−248131号)脱脂粉乳のような動物または植物性乳製品を加えて発酵させて発酵速度を速めるための乳酸菌発酵食品の製法が提案されている。   For example, Lactobacillus brevis (Japanese Patent Publication No. 58-15109) removes the heated odor of vegetable mixed juice, prepares food and drink with emphasis on not producing diacetyl, Lactobacillus casei No. 292869) is a preparation of fermented food that does not produce an acetic acid-like odor, and a combination of Lactobacillus bulgaricus and Streptococcus thermophilus (Japanese Patent Laid-Open No. 60-248131) animal or vegetable dairy products such as skim milk powder A method for producing a lactic acid bacteria fermented food for increasing the fermentation rate by adding fermenter has been proposed.

また、エンテロコッカス属菌及び必要に応じこれにラクトバチルス属菌を組み合わせ(特許第3124670号)焦げ臭や酸臭を改善して緑黄色野菜類を摂食できる技術も開示されている。
しかしながら、いまだ野菜類をフルーツのような風味として子供や老人が嗜好的且つ常用的に好適に食する食品素材は完成されておらず提供されていない。
In addition, enterococcus bacteria and, if necessary, a combination of Lactobacillus bacteria (Patent No. 312670) have also disclosed a technique that can eat green and yellow vegetables by improving the burning odor and acid odor.
However, food materials that can be eaten by children and the elderly in a tasteful and routine manner with a fruity flavor like vegetables have not yet been completed and are not provided.

特公昭58−15109号公報Japanese Patent Publication No.58-15109 特開平3−292869号公報JP-A-3-292869 特開昭60−248131号公報JP 60-248131 A 特許第3124670号公報Japanese Patent No. 312670

このような事情に鑑み、本発明者らは、野菜類などを手軽に摂取できるピューレ状食品用素材とすると同時に、味覚を改善しフルーツ風味で常用喫食するに優れた味覚となる、望ましい野菜類の食品用素材加工食品の提供について鋭意検討した結果、ペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌で炭水化物を8重量%以上含む野菜類を発酵させることが、風味改善なかんずくフルーツ風味として甘味に優れた食品用素材加工食品を提供することに極めて有効であることを見いだし、本発明を完成するに至った。   In view of such circumstances, the inventors of the present invention provide a puree food material that can be easily ingested with vegetables and the like, and at the same time, desirable vegetables that improve taste and have excellent taste for regular eating with a fruit flavor. As a result of earnest study on the provision of processed food materials for foods, fermenting vegetables containing 8% by weight or more of carbohydrates with lactic acid bacteria consisting of a combination of Pediococcus and Enterococcus spp. As a result, the present invention has been found to be extremely effective in providing processed food materials with excellent sweetness, and the present invention has been completed.

請求項1にかかる発明は、炭水化物を8重量%以上含む野菜の破砕物、磨砕物を、少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌で発酵させた乳酸菌発酵食品素材に関する。   The invention according to claim 1 relates to a lactic acid bacterium fermented food material obtained by fermenting a crushed vegetable product and a ground product containing 8% by weight or more of a carbohydrate with a lactic acid bacterium comprising at least a combination of Pediococcus and Enterococcus.

請求項2にかかる発明は、ペディオコッカス属菌がペディオコッカス・ペントサシュース及び/又はペディオコッカス・アシドラクティーである請求項1記載の乳酸菌発酵食品素材に関する。   The invention according to claim 2 relates to the lactic acid bacteria fermented food material according to claim 1, wherein the genus Pediococcus is Pediococcus pentosassus and / or Pediococcus acid lacty.

請求項3にかかる発明は、エンテロコッカス属菌がエンテロコッカス・フェシーム及び/又はエンテロコッカス・フェカーリスである請求項1又は2記載の乳酸菌発酵食品素材に関する。   The invention according to claim 3 relates to the lactic acid bacteria fermented food material according to claim 1 or 2, wherein the Enterococcus spp. Is Enterococcus faecium and / or Enterococcus faecalis.

請求項4にかかる発明は、前記乳酸菌がロイコノストック・メセンテロイデスを含むことを特徴とする請求項1乃至3記載の乳酸菌発酵食品素材に関する。   The invention according to claim 4 relates to the lactic acid bacteria fermented food material according to claims 1 to 3, wherein the lactic acid bacteria include Leuconostoc mesenteroides.

請求項5にかかる発明は、炭水化物を8重量%以上含む野菜が人参、小豆、大豆、ニンニク、とうもろこし、サツマイモ、かぼちゃ、ジャガイモ、サトイモ、枝豆、ソラマメ、ゴマ、ハス、栗などの野菜から少なくとも一種選択されてなる野菜であることを特徴とする請求項1乃至4記載の乳酸菌発酵食品素材に関する。   In the invention according to claim 5, the vegetable containing 8% by weight or more of carbohydrate is at least one kind of vegetables such as carrots, red beans, soybeans, garlic, corn, sweet potatoes, pumpkins, potatoes, taros, green soybeans, broad beans, sesame, lotus, chestnuts, etc. The lactic acid bacteria fermented food material according to claim 1, which is a selected vegetable.

請求項6にかかる発明は、炭水化物を8重量%以上含む野菜を必要に応じて剥皮し蒸煮し、この蒸煮物を破砕または磨砕して加水しゼリー状とし、このゼリー状物に少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌を加え加温しながら乳酸菌発酵させ、必要に応じ加熱処理することからなる乳酸菌発酵食品素材の製法に関する。   The invention according to claim 6 peels and steams vegetables containing 8% by weight or more of carbohydrates as necessary, crushes or grinds the steamed product to form a jelly form, and at least pedio is added to the jelly form. The present invention relates to a method for producing a lactic acid bacteria fermented food material comprising adding lactic acid bacteria comprising a combination of Coccus spp. And Enterococcus spp.

請求項7にかかる発明は、ペディオコッカス属菌がペディオコッカス・ペントサシュース及び/又はペディオコッカス・アシドラクティーである請求項6記載の乳酸菌発酵食品素材の製法に関する。   The invention according to claim 7 relates to a method for producing a fermented food material for lactic acid bacteria according to claim 6, wherein the genus Pediococcus is Pediococcus pentosassus and / or Pediococcus acid lacty.

請求項8にかかる発明は、エンテロコッカス属菌がエンテロコッカス・フェシーム及び/又はエンテロコッカス・フェカーリスである請求項6記載の乳酸菌発酵食品素材の製法に関する。   The invention according to claim 8 relates to a method for producing a lactic acid bacteria fermented food material according to claim 6, wherein the Enterococcus spp. Is Enterococcus faecium and / or Enterococcus faecalis.

請求項9にかかる発明は、前記乳酸菌がロイコノストック・メセンテロイデスを含むことを特徴とする請求項6記載の乳酸菌発酵食品素材の製法に関する。   The invention according to claim 9 relates to a method for producing a lactic acid bacteria fermented food material according to claim 6, wherein the lactic acid bacteria include Leuconostoc mesenteroides.

請求項10にかかる発明は、炭水化物を8重量%以上含む野菜が人参、小豆、大豆、ニンニク、とうもろこし、サツマイモ、かぼちゃ、ジャガイモ、サトイモ、枝豆、ソラマメ、ゴマ、ハス、栗などの野菜から少なくとも一種選択されてなる野菜であることを特徴とする請求項6記載の乳酸菌発酵食品素材の製法に関する。   The invention according to claim 10 is characterized in that the vegetable containing 8% by weight or more of carbohydrate is at least one kind of vegetables such as carrots, red beans, soybeans, garlic, corn, sweet potatoes, pumpkins, potatoes, taro, green soybeans, broad beans, sesame, lotus, chestnuts, etc. It is the vegetable by which it is selected, It is related with the manufacturing method of the lactic-acid-bacteria fermented food raw material of Claim 6.

請求項1にかかるこの発明は、炭水化物を8重量%以上含む野菜類の破砕物、磨砕物を少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌で発酵させた乳酸菌発酵食品素材であるから、炭水化物を8重量%以上含む野菜類を風味に優れた乳酸菌発酵食品素材とすることができる効果を奏する。   The invention according to claim 1 is a lactic acid bacteria fermented food material obtained by fermenting a crushed vegetable product and a ground product containing at least 8% by weight of a carbohydrate with a lactic acid bacterium comprising at least a combination of Pediococcus and Enterococcus. Therefore, there exists an effect which can use as a lactic-acid-bacteria fermented food material excellent in flavor the vegetables containing 8 weight% or more of carbohydrates.

請求項2にかかる発明は、ペディオコッカス属菌がペディオコッカス・ペントサシュース及び/又はペディオコッカス・アシドラクティーである請求項1記載の乳酸菌発酵食品素材であるから、耐塩性が高くまた発酵食品が炭酸ガスを発生せずしかも生育温度が高く且つ適応pH値が広く従っていろいろな種類の野菜類を乳酸菌発酵食品素材とすることができる効果を奏する。   The invention according to claim 2 is the lactic acid bacteria fermented food material according to claim 1, wherein the genus Pediococcus is Pediococcus pentosasseus and / or Pediococcus acid lacty, and therefore has high salt resistance and fermentation. The food does not generate carbon dioxide, has a high growth temperature, and has a wide adaptive pH value. Therefore, various kinds of vegetables can be used as a material for fermenting lactic acid bacteria.

請求項3にかかる発明は、エンテロコッカス属菌がエンテロコッカス・フェシーム及び/又はエンテロコッカス・フェカーリスである請求項1又は2記載の乳酸菌発酵食品素材であるから、エンテロコッカス属菌が元来人の体内即ち腸内乳酸菌であることより、この乳酸菌発酵食品素材を喫食すると整腸作用など人体に有効であるという効果、及び種々の野菜類をフルーツ風味及びコクのある風味を有する乳酸菌発酵食品素材とすることができる効果を奏する。   The invention according to claim 3 is the lactic acid bacteria fermented food material according to claim 1, wherein the Enterococcus genus is Enterococcus faecium and / or Enterococcus faecalis. Since it is a lactic acid bacterium, eating this lactic acid bacterium fermented food material is effective for the human body such as intestinal regulation, and various vegetables can be made into a lactic acid bacterium fermented food material having a fruity flavor and a rich flavor. There is an effect.

請求項4にかかる発明は、前記乳酸菌がロイコノストック・メセンテロイデスを含むことを特徴とする請求項1乃至3記載の乳酸菌発酵食品素材であるから、種々の野菜類をフルーツ風味及びコクのある風味がより増した乳酸菌発酵食品素材とすることができる効果を奏する。   The invention according to claim 4 is the lactic acid bacteria fermented food material according to claims 1 to 3, characterized in that the lactic acid bacteria include Leuconostoc mesenteroides, so that various vegetables have fruit flavors and rich flavors. The effect which can be made into the lactic-acid-bacteria fermented food material which increased more.

請求項5にかかる発明は、炭水化物を8重量%以上含む野菜が人参、小豆、大豆、ニンニク、とうもろこし、サツマイモ、かぼちゃ、ジャガイモ、サトイモ、枝豆、ソラマメ、ゴマ、ハス、栗などの野菜から少なくとも一種選択されてなる野菜であることを特徴とする請求項1乃至4記載の乳酸菌発酵食品素材であるから、加熱などの処理を要せずまた糖分を過剰に加えずに、種々の野菜類を風味改善してフルーツ風味を有する乳酸菌発酵食品素材とすることができる効果を奏する。   The invention according to claim 5 is characterized in that the vegetable containing 8% by weight or more of carbohydrate is at least one kind of vegetables such as carrots, red beans, soybeans, garlic, corn, sweet potatoes, pumpkins, potatoes, taros, green soybeans, broad beans, sesame, lotus, chestnuts, etc. The lactic acid bacteria fermented food material according to claim 1, wherein the lactic acid bacteria fermented food material is selected, and does not require treatment such as heating, and does not add excessive sugar, and flavors various vegetables. The effect which it can improve and can be set as the lactic-acid-bacteria fermented food material which has a fruit flavor is show | played.

請求項6にかかる発明は、炭水化物を8重量%以上含む野菜を必要に応じて剥皮し蒸煮し、この蒸煮物を破砕または磨砕して加水しゼリー状とし、このゼリー状物に少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌を加え加温しながら乳酸菌発酵させ、必要に応じ加熱処理することからなる乳酸菌発酵食品素材の製法であるから、炭水化物を8重量%以上含む野菜類を風味に優れた乳酸菌発酵食品素材として製造できる効果を奏する。   The invention according to claim 6 peels and steams vegetables containing 8% by weight or more of carbohydrates as necessary, crushes or grinds the steamed product to form a jelly form, and at least pedio is added to the jelly form. Vegetables containing 8% by weight or more of carbohydrates because it is a method for producing lactic acid bacteria fermented food materials by adding lactic acid bacteria consisting of a combination of Coccus spp. And Enterococcus spp. The effect which can manufacture lactic acid bacteria fermented food material excellent in flavor is produced.

請求項7にかかる発明は、ペディオコッカス属菌がペディオコッカス・ペントサシュース及び/又はペディオコッカス・アシドラクティーである請求項6記載の乳酸菌発酵食品素材の製法であるから、耐塩性が高くまた発酵食品が炭酸ガスを発生せずしかも生育温度が高く且つ適応pH値が広く従っていろいろな種類の野菜類を乳酸菌発酵食品素材として製造できる効果を奏する。   The invention according to claim 7 is a method for producing a fermented food material for lactic acid bacteria according to claim 6, wherein the genus Pediococcus is Pediococcus pentosassus and / or Pediococcus acid lacty, and has high salt resistance. In addition, the fermented food does not generate carbon dioxide, has a high growth temperature, and has a wide adaptive pH value, so that various kinds of vegetables can be produced as fermented food materials for lactic acid bacteria.

請求項8にかかる発明は、エンテロコッカス属菌がエンテロコッカス・フェシーム及び/又はエンテロコッカス・フェカーリスである請求項6記載の乳酸菌発酵食品素材の製法であるから、エンテロコッカス属菌が元来人の体内即ち腸内乳酸菌であることより、喫食すると整腸作用など人体に有効である乳酸菌発酵食品素材を製造できる効果、及び種々の野菜類をフルーツ風味及びコクのある風味を有する乳酸菌発酵食品素材とすることができる効果を奏する。   The invention according to claim 8 is the method for producing a lactic acid bacteria fermented food material according to claim 6, wherein the enterococcus is Enterococcus faecium and / or Enterococcus faecalis. Since it is a lactic acid bacterium, the effects of producing a lactic acid bacterium fermented food material that is effective for the human body such as intestinal regulation when eaten, and various vegetables can be made into a lactic acid bacterium fermented food material having a fruity flavor and a rich flavor. There is an effect.

請求項9にかかる発明は、前記乳酸菌がロイコノストック・メセンテロイデスを含むことを特徴とする請求項6記載の乳酸菌発酵食品素材の製法であるから、種々の野菜類をフルーツ風味及びコクのある風味がより増した乳酸菌発酵食品素材として製造できる効果を奏する。   The invention according to claim 9 is the method for producing a lactic acid bacterium fermented food material according to claim 6, wherein the lactic acid bacterium contains Leuconostoc mesenteroides, so that various vegetables have a fruit flavor and a rich flavor. The effect which can be manufactured as a lactic-acid-bacteria fermented food material which increased more.

請求項10にかかる発明は、炭水化物を8重量%以上含む野菜が人参、小豆、大豆、ニンニク、とうもろこし、サツマイモ、かぼちゃ、ジャガイモ、サトイモ、枝豆、ソラマメ、ゴマ、ハス、栗などの野菜から少なくとも一種選択されてなる野菜であることを特徴とする請求項6記載の乳酸菌発酵食品素材の製法であるから、加熱などの処理を要せずまた糖分を過剰に加えずに、種々の野菜類を風味改善してフルーツ風味を有する乳酸菌発酵食品素材として製造することができる効果を奏する。   The invention according to claim 10 is characterized in that the vegetable containing 8% by weight or more of carbohydrate is at least one kind of vegetables such as carrots, red beans, soybeans, garlic, corn, sweet potatoes, pumpkins, potatoes, taro, green soybeans, broad beans, sesame, lotus, chestnuts, etc. 7. The method for producing a lactic acid bacterium fermented food material according to claim 6, wherein the vegetable is a selected vegetable, so that various kinds of vegetables can be flavored without requiring treatment such as heating and without excessive addition of sugar. The effect which it can improve and can manufacture as a lactic-acid-bacteria fermented food raw material which has fruit flavor is produced.

以下、本発明に係る乳酸菌発酵食品素材及びその製法の実施例について詳細に説明する。
この発明で使用する炭水化物を8重量%以上含む野菜類とは、原料野菜類中の栄養成分として炭水化物を8重量%以上含んでいれば良く、例えば生であれば人参、小豆、大豆、ニンニク、とうもろこし、サツマイモ、かぼちゃ、ジャガイモ、サトイモ、枝豆、ソラマメ、ゴマ、ハス、栗などの野菜類、果実、種子などを挙げることができ、これらの野菜類からから少なくとも一種選択して使用すればよい。この発明においては、例えば、生の状態なら炭水化物が8重量%以下の野菜類でも、乾燥物あるいは漬物とした野菜類で炭水化物8重量%以上含むものも採用できる。この発明において、原料とする野菜類を炭水化物を8重量%以上含有する野菜類とする理由は、乳酸菌発酵に際して糖類の添加量をできるだけ減少させて摂食者の糖分摂取過多を防止するためである。
この発明においては係る特定の野菜類を原料とすることにより、砂糖などの糖類の添加量が、定法では15〜25%であるのに比べ、5〜15%とすることができる。
Hereinafter, the Example of the lactic-acid-bacteria fermented food material which concerns on this invention, and its manufacturing method is demonstrated in detail.
Vegetables containing 8% by weight or more of carbohydrates used in the present invention need only contain 8% by weight or more of carbohydrates as nutritional ingredients in raw vegetables. For example, if raw, carrots, red beans, soybeans, garlic, Examples include corn, sweet potato, pumpkin, potato, taro, edamame, broad bean, sesame, lotus, chestnut and other vegetables, fruits, seeds, etc. At least one kind selected from these vegetables may be used. In the present invention, for example, vegetables that are 8% by weight or less of carbohydrates in the raw state, or vegetables that are dried or pickled and that contain 8% by weight or more of carbohydrates can be employed. In this invention, the reason why the vegetables used as raw materials are vegetables containing 8% by weight or more of carbohydrates is to prevent excessive intake of sugar by the intake person by reducing the amount of sugar added as much as possible during lactic acid bacteria fermentation. .
In this invention, by using the specific vegetables as a raw material, the addition amount of sugars such as sugar can be 5 to 15% as compared with 15 to 25% in a conventional method.

この発明においては、これら野菜類を常法に従って、生のものを洗浄後、必要に応じて剥皮し、適宜のサイズに切断、スライス等処理をし、その後蒸煮する。蒸煮時間は野菜類などの種類、蒸気温度、圧力などによって異なるが、例えばカボチャを1気圧、100℃で蒸煮する場合は15分から30分程度処理するのが望ましい。   In the present invention, these vegetables are washed in accordance with a conventional method, and then peeled off if necessary, cut into an appropriate size, processed into slices, and then steamed. The cooking time varies depending on the type of vegetables, steam temperature, pressure, etc. For example, when the pumpkin is cooked at 1 atm and 100 ° C., it is desirable to treat it for 15 to 30 minutes.

この発明においては、蒸煮後の野菜類を、野菜類と同量程度80〜100%の重量の水分を加えながらあるいは破砕、摩砕し、または処理後加えゼリー状とする。破砕、摩砕工程はクラッシャー、ミキサー、マスコロイダー、パルパーフィニシャーなどを用いて行う。加えて遠心分離、フィルタープレス等で搾汁して使用してもよく、搾汁を減圧濃縮などの公知の方法で濃縮して使用してもよい。発酵工程時に加水してゼリー状としても良い。
尚、これらは微生物汚染や成分の変質を受けやすいため、冷凍保存するのが好ましい。
野菜類のゼリーに濃縮物を混合して用いる場合は、これらの処理前に混合しても、また、処理後に混合してもよく、発酵の際に混合することもできる。
In this invention, the vegetables after cooking are made into a jelly form while adding or crushing, grinding, or processing after adding water of about 80 to 100% by weight of the vegetables. The crushing and grinding processes are performed using a crusher, a mixer, a mass collider, a pulper finisher, or the like. In addition, the juice may be used after being squeezed by centrifugation, a filter press or the like, or the juice may be used after being concentrated by a known method such as vacuum concentration. It may be hydrated during the fermentation process to form a jelly.
Since these are susceptible to microbial contamination and component alteration, they are preferably stored frozen.
When the concentrate is mixed and used in the vegetable jelly, it may be mixed before these treatments, after the treatment, or may be mixed during the fermentation.

このゼリー状の破砕物、磨砕物に、糖を5〜15%加え、少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌を植え付け発酵させる。
スターターとなる乳酸菌は、それぞれ酵母エキス、ペプトン等を添加した10〜15%還元脱脂乳培地等の公知の培地を用いて常法どおりに培養し、スターターを調製する。
発酵は、例えば、スターターをゼリー状の野菜類に対し0.1〜2.0重量%接種して行えばよい。
Sugar is added to the jelly-like crushed material and ground product in an amount of 5 to 15%, and lactic acid bacteria comprising at least a combination of Pediococcus and Enterococcus are planted and fermented.
A lactic acid bacterium serving as a starter is cultured in a conventional manner using a known medium such as a 10-15% reduced skim milk medium supplemented with yeast extract, peptone, etc. to prepare a starter.
For example, the fermentation may be performed by inoculating a jelly-like vegetable with 0.1 to 2.0% by weight of a starter.

この発明においては、乳酸菌発酵食品素材の発酵条件は、攪拌しながら菌を植え付け次いで15〜40℃で36〜72時間望ましくは48時間程度静置させて行う。   In this invention, the fermentation conditions of the lactic acid bacteria fermented food material are carried out by planting the bacteria while stirring and then allowing to stand at 15 to 40 ° C. for 36 to 72 hours, preferably about 48 hours.

この発明で使用するペディオコッカス属菌は、耐塩性が高くまた発酵食品が炭酸ガスを発生せずしかも生育温度が高く且つ適応pH値が広いものである。従っていろいろな種類の野菜類を乳酸菌発酵食品素材としたときに保存性が高くしかも風味に優れる。   The Pediococcus spp. Used in this invention have high salt tolerance, fermented foods do not generate carbon dioxide, have a high growth temperature, and have a wide adaptive pH value. Therefore, when various kinds of vegetables are used as lactic acid bacteria fermented food materials, they have high storage stability and excellent flavor.

この発明においては、ペディオコッカス属菌のうちペディオコッカス・ペントサシュース及び/又はペディオコッカス・アシドラクティーを使用するのが好ましい。その理由は、ペディオコッカス属菌の中でも特に耐塩性が高くまた発酵食品が炭酸ガスを発生せずしかも生育温度が高く且つ適応pH値が広いからであり、従っていろいろな種類の野菜類を乳酸菌発酵食品素材とすることができる効果を奏する。   In the present invention, it is preferable to use Pediococcus pentosaceus and / or Pediococcus acid lacti among the genus Pediococcus. The reason is that among the genus Pediococcus, the salt resistance is particularly high, the fermented food does not generate carbon dioxide, the growth temperature is high, and the adaptive pH value is wide. The effect which can be used as a fermented food material is produced.

この発明で使用するエンテロコッカス属に属する細菌は、人の腸内に常在しているグラム陽性球菌である。このエンテロコッカス属に属する乳酸菌は、6.5%塩化ナトリウムを含む液体培地、pH9.6の液体培地で増殖開始できる点で、他のストレプトコッカス属菌やラクトコッカス属菌と区別され、現在ストレプトコッカスとは独立した属として分類されている。エンテロコッカス・フェカーリスは整腸作用があるため、その凍結乾燥物は整腸剤として広く販売されている。
この発明において、エンテロコッカス・フェカーリス、エンテロコッカス・フェシームは野菜類の発酵風味を改善しフルーツ風味及びコクのある風味をつくり好ましい。
The bacteria belonging to the genus Enterococcus used in the present invention are gram-positive cocci that are resident in the human intestine. Lactic acid bacteria belonging to the genus Enterococcus are distinguished from other Streptococcus and Lactococcus bacteria in that they can start growing in a liquid medium containing 6.5% sodium chloride and a pH 9.6 liquid medium. Classified as an independent genus. Since Enterococcus faecalis has an intestinal action, its freeze-dried product is widely sold as an intestinal preparation.
In this invention, Enterococcus faecalis and Enterococcus faecium are preferable because they improve the fermented flavor of vegetables and produce a fruit flavor and rich flavor.

この発明において乳酸菌としてはペディオコッカス属菌とエンテロコッカス属菌の組み合わせにロイコノストック・メセンテロイデスを組み合わせる。
ロイコノストック・メセンテロイデスは糖やたんぱく質を分解してうまみ、コクのある風味及びフルーツ風味を出す効果を奏する。
In this invention, as a lactic acid bacterium, a combination of Pediococcus and Enterococcus is combined with Leuconostoc mesenteroides.
Leuconostoc mesenteroides has the effect of decomposing sugars and proteins to produce a delicious, rich flavor and fruit flavor.

この発明において発酵温度は30〜40℃、好ましくは35〜38℃で行うのが望ましい。発酵時間は豆類、イモ類などの野菜類の種類により勘案すればよく、通常12〜36時間で、pH値、生成した乳酸菌量を目安に、所望の最終製品に適した発酵の終点を定め決定する。   In this invention, the fermentation temperature is 30 to 40 ° C, preferably 35 to 38 ° C. Fermentation time should be taken into account depending on the type of vegetables such as beans and potatoes, and is usually determined in 12 to 36 hours by determining the end point of fermentation suitable for the desired final product based on the pH value and the amount of lactic acid bacteria produced To do.

この発明において発酵の終点は発酵物がpH4.0〜4.5、生菌数が10となった段階で発酵終点とするのが好ましい。発酵物の性状はクリーム状あるいはピューレ状の乳酸菌発酵食品素材となる。この終点後必要に応じて130℃、3分間程度の加熱処理により殺菌し、ソフトタイプとし加工食品の素材とする。最終食品としては、例えば濃縮、希釈などを経てあるいはそのままでヨーグルト、ジャム、ピューレなどの調味料とすることができる。添加物をできるだけ加えないことがこの発明においては望ましいが、場合によっては従来公知の賦形剤、増量剤、着色剤、防腐剤などの添加物を加えても良い。
この発明においては乳酸発酵食品素材の最終物に対し加熱処理を行わない場合には冷凍し生菌の活動を停止させるのが望ましい。
この冷凍食品はハードタイプとなり生菌のまま人体に喫食されるので整腸作用が好ましく発揮できるため、アイスクリーム食品などの冷果に加工するのが望ましい。
End point of the fermentation in the present invention the fermentation product has pH 4.0-4.5, the number of viable bacteria preferably fermented ending at the stage became 10 7. The nature of the fermented product is a creamy or puree lactic acid bacteria fermented food material. After this end point, if necessary, it is sterilized by heat treatment at 130 ° C. for about 3 minutes to form a soft type and a processed food material. As the final food, for example, it can be used as a seasoning such as yogurt, jam, puree after being concentrated or diluted. Although it is desirable in the present invention to add as few additives as possible, conventionally known additives such as excipients, extenders, colorants, preservatives may be added.
In the present invention, when the final product of the lactic acid fermented food material is not subjected to heat treatment, it is desirable to freeze and stop the activity of viable bacteria.
Since this frozen food becomes a hard type and is eaten by the human body in the form of viable bacteria, it is preferable to process it into a cold fruit such as an ice cream food because it can preferably exert an intestinal regulating action.

得られた本発明の乳酸菌発酵食品素材は、そのまま飲食用に供することもでき、また、常法に従い、各種の飲食品、例えばヨーグルト、ピューレ、ゼリー、パン用のスプレッド類、調味料、アイスクリームとすることができる。   The obtained lactic acid bacteria fermented food material of the present invention can be used for food and drink as it is, and according to conventional methods, various foods and drinks such as yogurt, puree, jelly, bread spreads, seasonings, ice cream It can be.

(実施例)
以下、実施例および比較例の試験を挙げて本発明をさらに詳しく説明するが、本発明はこれらに限定されるものではない。「%」は重量%を意味する。
実験例及び比較例:以下に示す種々の炭水化物8%以上と以下の野菜類について、種々の乳酸菌を用いて発酵試験を行った。
(Example)
EXAMPLES Hereinafter, although the test of an Example and a comparative example is given and this invention is demonstrated in more detail, this invention is not limited to these. “%” Means% by weight.
Experimental Examples and Comparative Examples: Fermentation tests were performed using various lactic acid bacteria on the following various carbohydrates 8% or more and the following vegetables.

(1)ジャガイモ(炭水化物16.9%)、サツマイモ(炭水化物31.53%)、とうもろこし(炭水化物16.83%)、にんにく(炭水化物26.3%)、ソラマメ(炭水化物 55.93%)、小豆(炭水化物58.73%)、かぼちゃ(炭水化物10.93%)、にんじん(炭水化物9.13%)、栗(炭水化物36.9%)、サトイモ(炭水化物13.1%)の炭水化物8%以上の野菜類と、ブロッコリー(炭水化物5.1%)を炭水化物8%以下の比較例にかかる野菜類として試験した。 (1) Potato (Carbohydrate 16.9%), Sweet Potato (Carbohydrate 31.53%), Corn (Carbohydrate 16.83%), Garlic (Carbohydrate 26.3%), Broad Bean (Carbohydrate 55.93%), Red Bean ( Carbohydrate 58.73%), Pumpkin (Carbohydrate 10.93%), Carrot (Carbohydrate 9.13%), Chestnut (Carbohydrate 36.9%), Taro (Carbohydrate 13.1%) Broccoli (5.1% carbohydrate) was tested as a vegetable according to a comparative example having 8% or less carbohydrate.

(2)野菜類を100℃で20分間蒸煮し、家庭用ミキサーで粉砕し等量の水を加えクリーム状にした。
このクリーム状のものに10%の砂糖又は果糖を加え、下記表に示す乳酸菌を0.3%攪拌しながら添加した。発酵温度28℃で36時間乃至48時間培養した。pH4.0付近を終点として、発酵を終了した。終了時にpH値、香り、風味、味を試験した。
(2) Vegetables were cooked at 100 ° C. for 20 minutes, pulverized with a home-use mixer, and added with an equal amount of water to form a cream.
10% sugar or fructose was added to the creamy product, and the lactic acid bacteria shown in the following table were added with stirring by 0.3%. The culture was performed at a fermentation temperature of 28 ° C. for 36 to 48 hours. Fermentation was terminated with an end point at around pH 4.0. At the end, the pH value, aroma, flavor and taste were tested.

(味覚試験)
味覚試験は官能評価で行った。10名に喫食させ味覚を5段階官能評価で行い、そのトータルスコアで表した。
5:非常に良い、4:良い、3:普通、2:悪い、1:非常に悪い
以下結果をまとめて表1、表2に示す。
(Taste test)
The taste test was conducted by sensory evaluation. Ten people were allowed to eat and taste was evaluated by a five-step sensory evaluation and expressed as a total score.
5: Very good, 4: Good, 3: Normal, 2: Bad, 1: Very bad The results are summarized in Tables 1 and 2 below.

以上の結果から明らかなように、炭水化物を8重量%以上含む野菜類の破砕物、磨砕物を少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせ更にはロイコノストック・メセンテロイデスを組み合わせて発酵させた乳酸菌発酵食品素材は、いずれも野菜類でありながら味覚、風味、香りに優れフルーツ風味の食品素材となる効果を奏している。これに比して、炭水化物を8重量%以下の野菜類例えばブロッコリーは、食品素材として劣ることが明らかである。   As is clear from the above results, the crushed vegetable product containing 8% by weight or more of carbohydrates and the ground product are fermented by combining at least Pediococcus and Enterococcus, and combining Leuconostoc mesenteroides. All of the lactic acid bacteria fermented food materials have excellent taste, flavor, and fragrance despite being vegetables, and have the effect of becoming a fruit-flavored food material. On the other hand, it is clear that vegetables with 8% by weight or less of carbohydrates such as broccoli are inferior as food materials.

本発明は、炭水化物を8重量%以上含む野菜類の破砕物、磨砕物を、少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌で発酵させて野菜類をフルーツ風味に処理し例えばピューレ状食品用素材としたうえアイスクリーム、ヨーグルト、ジャム、ピューレなどの食品に加工できる乳酸菌発酵食品素材及びその製法として用いることができる。   In the present invention, a vegetable pulverized product and a ground product containing 8% by weight or more of carbohydrates are fermented with a lactic acid bacterium comprising at least a combination of Pediococcus and Enterococcus, and the vegetables are processed to have a fruit flavor. It can be used as a raw material for puree foods, and can be used as a fermented food material for lactic acid bacteria that can be processed into foods such as ice cream, yogurt, jam, and puree, and a production method thereof.

Claims (10)

炭水化物を8重量%以上含む野菜の破砕物、磨砕物を、少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌で発酵させた乳酸菌発酵食品素材。   A lactic acid bacteria fermented food material obtained by fermenting a crushed vegetable product and a ground product containing 8% by weight or more of a carbohydrate with a lactic acid bacterium comprising at least a combination of Pediococcus and Enterococcus. ペディオコッカス属菌がペディオコッカス・ペントサシュース及び/又はペディオコッカス・アシドラクティーである請求項1記載の乳酸菌発酵食品素材。   The lactic acid bacteria fermented food material according to claim 1, wherein the Pediococcus spp. Is Pediococcus pentosassus and / or Pediococcus acid lacty. エンテロコッカス属菌がエンテロコッカス・フェシーム及び/又はエンテロコッカス・フェカーリスである請求項1又は2記載の乳酸菌発酵食品素材。   The lactic acid bacteria fermented food material according to claim 1 or 2, wherein the Enterococcus spp. Is Enterococcus faecium and / or Enterococcus faecalis. 前記乳酸菌がロイコノストック・メセンテロイデスを含むことを特徴とする請求項1乃至3記載の乳酸菌発酵食品素材。   4. The lactic acid bacteria fermented food material according to claim 1, wherein the lactic acid bacteria include Leuconostoc mesenteroides. 炭水化物を8重量%以上含む野菜が人参、小豆、大豆、ニンニク、とうもろこし、サツマイモ、かぼちゃ、ジャガイモ、サトイモ、枝豆、ソラマメ、ゴマ、ハス、栗などの野菜から少なくとも一種選択されてなる野菜であることを特徴とする請求項1乃至4記載の乳酸菌発酵食品素材。   Vegetables containing 8% by weight or more of carbohydrates must be at least one selected from vegetables such as carrots, red beans, soybeans, garlic, corn, sweet potatoes, pumpkins, potatoes, taros, edamame, broad beans, sesame, lotus, chestnuts, etc. The lactic acid bacteria fermented food material according to claim 1, wherein: 炭水化物を8重量%以上含む野菜を必要に応じて剥皮し蒸煮し、この蒸煮物を破砕または磨砕して加水しゼリー状とし、このゼリー状物に少なくともペディオコッカス属菌とエンテロコッカス属菌との組み合わせからなる乳酸菌を加え加温しながら乳酸菌発酵させ、必要に応じ加熱処理することからなる乳酸菌発酵食品素材の製法。   Vegetables containing 8% by weight or more of carbohydrates are peeled and steamed as necessary, and the cooked product is crushed or ground to make a jelly form, and this jelly form contains at least Pediococcus and Enterococcus spp. A method for producing a lactic acid bacterium fermented food material comprising adding a lactic acid bacterium comprising a combination of lactic acid bacteria and heating the lactic acid bacterium while heating, and if necessary heat-treating. ペディオコッカス属菌がペディオコッカス・ペントサシュース及び/又はペディオコッカス・アシドラクティーである請求項6記載の乳酸菌発酵食品素材の製法。   The method for producing a lactic acid bacterium fermented food material according to claim 6, wherein the genus Pediococcus is Pediococcus pentosassus and / or Pediococcus acid lacty. エンテロコッカス属菌がエンテロコッカス・フェシーム及び/又はエンテロコッカス・フェカーリスである請求項6記載の乳酸菌発酵食品素材の製法。   The method for producing a lactic acid bacteria fermented food material according to claim 6, wherein the Enterococcus spp. Is Enterococcus faecium and / or Enterococcus faecalis. 前記乳酸菌がロイコノストック・メセンテロイデスを含むことを特徴とする請求項6記載の乳酸菌発酵食品素材の製法。   The method for producing a lactic acid bacteria fermented food material according to claim 6, wherein the lactic acid bacteria include Leuconostoc mesenteroides. 炭水化物を8重量%以上含む野菜が人参、小豆、大豆、ニンニク、とうもろこし、サツマイモ、かぼちゃ、ジャガイモ、サトイモ、枝豆、ソラマメ、ゴマ、ハス、栗などの野菜から少なくとも一種選択されてなる野菜であることを特徴とする請求項6記載の乳酸菌発酵食品素材の製法。   Vegetables containing 8% by weight or more of carbohydrates must be at least one selected from vegetables such as carrots, red beans, soybeans, garlic, corn, sweet potatoes, pumpkins, potatoes, taros, edamame, broad beans, sesame, lotus, chestnuts, etc. The method for producing a lactic acid bacteria fermented food material according to claim 6.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014128216A (en) * 2012-12-28 2014-07-10 Snow Shoji Kk Lactic acid bacterium-fermented food material and its manufacturing method
JP2017201921A (en) * 2016-05-10 2017-11-16 株式会社アキモ Vegetable-filled wheat base food product and method for producing the same
CN107529782A (en) * 2015-04-24 2018-01-02 通用磨坊公司 Dairy product substitute based on beans and include its consumer food
JP2018093848A (en) * 2016-12-13 2018-06-21 一興 石原 Fermentation gel by soybean as raw material

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005218324A (en) * 2004-02-04 2005-08-18 Eromlife Co Ltd Pediococcus pentosaceus erom101, its mutant or variant, immunopotentiating composition, anticancer composition, antimicrobial composition, composition for controlling intestinal function, composition for live bacterium agent, composition for feed, composition for food addition and fermented product containing them, method for culturing them and method for controlling microbial growth
JP2008245573A (en) * 2007-03-30 2008-10-16 Sunstar Inc Method for producing lactic acid fermented substance of vegetable crushed material

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005218324A (en) * 2004-02-04 2005-08-18 Eromlife Co Ltd Pediococcus pentosaceus erom101, its mutant or variant, immunopotentiating composition, anticancer composition, antimicrobial composition, composition for controlling intestinal function, composition for live bacterium agent, composition for feed, composition for food addition and fermented product containing them, method for culturing them and method for controlling microbial growth
JP2008245573A (en) * 2007-03-30 2008-10-16 Sunstar Inc Method for producing lactic acid fermented substance of vegetable crushed material

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014128216A (en) * 2012-12-28 2014-07-10 Snow Shoji Kk Lactic acid bacterium-fermented food material and its manufacturing method
CN107529782A (en) * 2015-04-24 2018-01-02 通用磨坊公司 Dairy product substitute based on beans and include its consumer food
AU2016250840B2 (en) * 2015-04-24 2020-06-04 General Mills, Inc. Legume-based dairy substitute and consumable food products incorporating same
JP2017201921A (en) * 2016-05-10 2017-11-16 株式会社アキモ Vegetable-filled wheat base food product and method for producing the same
JP2018093848A (en) * 2016-12-13 2018-06-21 一興 石原 Fermentation gel by soybean as raw material

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