JP2011505970A5 - - Google Patents

Claims (25)

A scaffold for use in an endodontic regeneration method comprising the following steps (a) to (d).
(A) removing unhealthy or infected dental pulp tissue from the root canal system (b) performing revascularization of the root canal system (c) a scaffold that may contain pulp progenitor cells and / or growth factors inserting into the root canal system and, and (d) prevent infection, to apply the post-operative sealant coronally access and / or apical access to the root canal step The scaffold according to claim 1, which is a rigid scaffold or an injectable scaffold . The scaffold according to claim 1 or 2 , wherein revascularization is achieved by causing blood flow into the root canal system by manipulating the apex with an instrument or removing the apex. The scaffold according to claim 1 or 2 , wherein the root canal system is disinfected using an intraperitoneal perfusate and an antibiotic to enhance revascularization. The scaffold according to any one of claims 1 to 4, wherein the pulp progenitor cells are obtained from autologous cells derived from an oral mucosa biopsy. The scaffold according to any one of claims 1 to 4, wherein the dental pulp progenitor cells are derived from a purified allogeneic dental pulp stem cell line that is expected to have no disease and pathogen. The scaffold according to any one of claims 1 to 4, wherein the dental pulp progenitor cells are derived from heterologous dental pulp stem cells grown in a laboratory. The scaffold according to any one of claims 1 to 4, wherein the dental pulp progenitor cells are obtained from autologous cells derived from umbilical cord stem cells. The scaffold according to any one of claims 1 to 4, wherein the pulp progenitor cells are obtained from extracted or in situ deciduous or permanent teeth and / or surrounding oral tissues. Used as pulp progenitor cells that are configured in a three-dimensional scaffold to support the organization and vascularization of the cells, scaffolds of any of claims 1-9. Three-dimensional scaffold is a porous polymer scaffolds seeded dental pulp progenitor cells to produce a pulp construct, scaffold of claim 10. The scaffold according to any one of claims 1 to 11, wherein the scaffold further comprises nutrients for promoting cell survival and proliferation, and antibiotics. The scaffold according to any one of claims 1 to 12, wherein the scaffold comprises a dentin fragment or a polymer hydrogel . An endodontic kit comprising an implantable scaffold matrix, an antiseptic solution, and isolated pulp cells. The kit according to claim 14, further comprising a cleaning solution that is an acid or a chelating agent. The kit according to claim 14 or 15 , further comprising an endodontic file. The kit according to any one of claims 14 to 16 , further comprising a perfusate. Transforming growth factor β family member, bone morphogenetic protein, insulin-like growth factor-I or -II, colony stimulating factor, epidermal growth factor, fibroblast growth factor, insulin-like growth factor-I or -II, interleukin The kit according to any one of claims 14 to 17 , further comprising a cell growth factor selected from the group consisting of IL-1 to IL-13, platelet-derived growth factor, and nerve growth factor. The kit according to any one of claims 14 to 18 , wherein the implantable scaffold matrix is a hydrogel. The kit according to any one of claims 14 to 19 , wherein the implantable scaffold matrix comprises a material selected from the group consisting of collagen, fibrin, chitosan, and glycosaminoglycan. The kit according to any one of claims 14 to 19 , wherein the implantable scaffold matrix comprises a material selected from the group consisting of polylactic acid, polyglycolic acid, and polycaprolactone. The kit according to any one of claims 14 to 18, wherein the implantable scaffold matrix is platelet-rich plasma, blood, or any serum product. The kit according to any one of claims 14 to 22 , wherein the implantable scaffold matrix comprises an antibiotic. The kit according to any one of claims 14 to 23 , wherein the isolated dental pulp cell is a stem cell. The kit according to any one of claims 14 to 24, wherein the isolated dental pulp cell expresses at least one of von Willebrand factor CD146, α-smooth muscle actin, and 3G5 protein.