JP2011236240A - Collagen synthesis-promoting amino acid composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an amino acid composition having excellent collagen synthesis-promoting action.SOLUTION: There is provided a collagen synthesis-promoting amino acid composition comprising 10-40 pts.mass of L-glutamine, 5-20 pts.mass of L-valine, 8-30 pts.mass of L-isoleucine and 10-35 pts.mass of L-leucine.

Description

  The present invention specifies 5 or 4 specific amino acids, namely L-arginine and / or L-glutamine, and 5 or 4 amino acids of L-valine, L-isoleucine and L-leucine. The present invention relates to a collagen synthesis-promoting amino acid composition characterized in that it is contained in a proportion, and to an amino acid composition useful for preventing skin aging, osteoporosis, tendon, ligament regeneration promotion and wound or burn healing related to collagen synthesis. is there.

  Collagen, the most abundant protein in the body, plays a very important role as a connective tissue as a component of the extracellular matrix. Examples of tissues rich in collagen include skin, bone tissue, tendons, and ligaments. These tissues cause wrinkles, sagging, osteoporosis and the like with aging, but it is thought that a decrease in tissue collagen amount and degeneration are major factors.

  Collagen synthesis is known to be promoted by the action of vitamins, hormones, cytokines, etc., but amino acids that are protein substrates are also known to promote collagen synthesis. In studies using fibroblasts, it has been reported that glutamine promotes collagen synthesis (Non-Patent Document 1). In studies using humans and animals, arginine itself, arginine, and β-hydroxy-β- are used in wound healing. There are reports that a mixture of methylbutyric acid and glutamine promotes collagen synthesis at the wound site (Patent Documents 1 and 2, and Non-Patent Document 2).

  Collagen proteins and collagen composition amino acid compositions are commercially available as products that promote collagen synthesis, but their effects are not clear, and excellent amino acids and amino acid compositions are highly desired. .

US005733884A US20030091601A1

G. Bellon et. al, Biochimica Biophysica Acta, 1995, 1268, 311-323. J. et al. Z. Williams et. al, Anals of surgery, 2002, 369-375.

  Under the background of the aforementioned prior art, an object of the present invention is to obtain an amino acid composition having an excellent collagen synthesis promoting action.

  As a result of diligent research to solve the problems described in the preceding paragraph, the present inventor obtained L-arginine and / or L-glutamine, and 5 or 4 amino acids of L-valine, L-isoleucine and L-leucine. The inventors have found that an amino acid composition containing a specific ratio promotes collagen synthesis, and has completed the present invention.

  That is, the present invention is characterized by containing a specific amino acid at a specific ratio, that is, 10 to 40 parts by mass of L-arginine and / or 10 to 40 parts by mass of L-glutamine, and L- A collagen synthesis promoting amino acid composition comprising 5 to 20 parts by mass of valine, 8 to 30 parts by mass of L-isoleucine, and 10 to 35 parts by mass of L-leucine, and an amino acid composition for preventing skin aging having the same composition , Osteoporosis-suppressing amino acid composition, tendon, ligament regeneration promoting amino acid composition, and wound or burn healing amino acid composition.

  According to the specific 5 or 4 amino acid composition of the present invention, synthesis of collagen in the body such as skin and bone tissue is promoted.

The change of the collagen synthesis rate by an amino acid composition is shown.

  The amino acid composition of the present invention comprises 10 to 40 parts by mass of L-arginine and / or 10 to 40 parts by mass of L-glutamine, 5 to 20 parts by mass of L-valine, and 8 to 30 parts by mass of L-isoleucine. An amino acid composition comprising 10 to 35 parts by mass of L-leucine. The amino acids used in the present invention may be peptide bodies containing those amino acids.

  These amino acid compositions may be blended with appropriate additives such as carbohydrates, lipids, proteins, vitamins, minerals such as vitamins, or any combination thereof. In that case, it is also possible to combine with an excipient, a flavoring agent, a pigment | dye, etc. The amino acid composition of the present invention thus produced can be put into circulation as it is, that is, in the form of a powder or liquid mixture. It can also be distributed in the form of supplements, seasonings, processed foods, pharmaceuticals such as infusions.

  The amino acid composition of the present invention can be administered intravenously as well as by oral administration. In the case of intravenous injection, it goes without saying that the preparation excludes additives that are not suitable for intravenous injection.

  Further, the dose of the amino acid composition of the present invention will be described. The amino acid composition was administered to the rat vein, and the blood amino acid concentration was increased and the amino acid composition was orally administered when promotion of collagen synthesis was observed. In consideration of the case where the increase in blood concentration is about the same, it can be about 5 to 12 g per day for an adult regardless of oral or intravenous injection.

  The amino acid composition of the present invention is administered in the above-described administration method and dosage, for example, by administration at a time when there is a possibility that symptoms of skin aging or osteoporosis appear, or when already developed. By doing so, it is possible to prevent the onset or suppress the progression of symptoms and further improve the symptoms.

  A protein-free diet (0% casein) was given to SD (IGS) male rats consisting of 4 to 6 rats in each group for 1 week before use in the experiment. As experimental groups for administering amino acids, 1) physiological saline, 2) L-glutamine, 3) L-arginine, 4) three kinds of branched chain amino acid compositions (L-leucine, L-isoleucine, and L-valine are generic names). 5) 12 amino acid compositions (L-isoleucine, L-leucine, L-valine, L-glutamine, L-histidine, L-lysine, L-phenylalanine, L-proline, L-threonine, 6) Collagen composition amino acid composition (L-isoleucine, L-leucine, L-valine, L-histidine, L-lysine, L-phenylalanine, L-proline, L) -Hydroxypurine, L-threonine, L-methionine, L-alanine, L-aspartic acid, L-glutamic acid, -Glycine, L-serine and L-arginine), 7) 5 amino acid compositions (L-leucine, L-isoleucine, L-valine, L-glutamine and L-arginine), 8) 4 amino acid compositions 1 ( L) -leucine, L-isoleucine, L-valine and L-glutamine), 9) 4 amino acid composition 2 (L-leucine, L-isoleucine, L-valine and L-arginine), 10) 2 amino acid composition A total of 10 groups (L-arginine and L-glutamine) were provided. The amino acid composition and administration concentration are shown in Table 1 below.

  The administration solution was continuously administered to the jugular vein for about 3 and a half hours. The collagen synthesis rate was determined by measuring the labeling rate of the stable isotope L-phenylalanine incorporated into the collagen protein within a certain time using the stable isotope L-phenylalanine. The tissue used was skin, and the collagen used was a nascent collagen fraction dissolved in a neutral salt solution. The results are shown in FIG.

  The physiological saline administration group was used as a control, and the test was carried out by t-test. In the figure, * represents p <0.05. The calculation method is shown in the following calculation formula (1).

  FIG. 1 shows changes in collagen synthesis rate. Collagen synthesis rate is determined by administering 5 amino acid compositions (BCAA + Arg + Gln in the figure), 4 amino acid compositions 1 (BCAA + Gln in the figure) or 4 amino acid compositions 2 (BCAA + Arg in the figure). Significant acceleration was observed for the group to which (PF in the figure) was administered. In addition, in administration of a branched chain amino acid composition (BCAA in the figure), L-arginine (Arg in the figure), L-glutamine (Gln in the figure), L-arginine + L-glutamine mixture (Arg + Gln in the figure) Since the recovery was not recognized and the rate of collagen synthesis was accelerated compared to the 12 amino acid compositions (AVP in the figure) and the collagen composition amino acid composition, these 5 or 4 amino acid compositions were It was shown to have a very good effect on promoting the synthesis of collagen.

Claims (8)

(A) L-glutamine, (b) L-valine, (c) L-isoleucine and (d) L-leucine amino acids as active ingredients, the ratio of the amino acids described in (a) to (d) below An amino acid-containing skin collagen synthesis promoter, characterized by comprising:
(A) 10 to 40 parts by weight (b) 5 to 20 parts by weight (c) 8 to 30 parts by weight (d) 10 to 35 parts by weight (A) L-glutamine, (b) L-valine, (c) L-isoleucine and (d) L-leucine amino acids as active ingredients, the ratio of the amino acids described in (a) to (d) below An amino acid-containing skin aging inhibitor, characterized by being a part by weight.
(A) 10 to 40 parts by weight (b) 5 to 20 parts by weight (c) 8 to 30 parts by weight (d) 10 to 35 parts by weight The amino acid consists of (a) L-glutamine, (b) L-valine, (c) L-isoleucine and (d) L-leucine, and the ratio of the amino acids is described in the following (a) to (d) Skin collagen synthesis promoter characterized by being a part.
(A) 10 to 40 parts by weight (b) 5 to 20 parts by weight (c) 8 to 30 parts by weight (d) 10 to 35 parts by weight The amino acid consists of (a) L-glutamine, (b) L-valine, (c) L-isoleucine and (d) L-leucine, and the ratio of the amino acids is described in the following (a) to (d) A skin aging inhibitor characterized by being a part.
(A) 10 to 40 parts by weight (b) 5 to 20 parts by weight (c) 8 to 30 parts by weight (d) 10 to 35 parts by weight   The skin collagen synthesis promoter according to claim 1 or 3 for addition to foods and drinks, supplements or pharmaceuticals.   The anti-aging agent for skin according to claim 2 or 4, which is added to foods, drinks, supplements or pharmaceuticals. A step of adding (a) L-glutamine, (b) L-valine, (c) L-isoleucine and (d) L-leucine as amino acids, wherein the ratio of the amino acids is as follows (a) to (d) The manufacturing method of the food-drinks, supplement, or pharmaceutical for skin collagen synthesis which are the weight part of description.
(A) 10 to 40 parts by weight (b) 5 to 20 parts by weight (c) 8 to 30 parts by weight (d) 10 to 35 parts by weight A step of adding (a) L-glutamine, (b) L-valine, (c) L-isoleucine and (d) L-leucine as amino acids, wherein the ratio of the amino acids is as follows (a) to (d) The manufacturing method of the food-drinks, supplement, or pharmaceutical for skin aging prevention which are the weight part of description.
(A) 10 to 40 parts by weight (b) 5 to 20 parts by weight (c) 8 to 30 parts by weight (d) 10 to 35 parts by weight

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