JP2011223904A - Cell-separating apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve therapeutic effects by a final product while guaranteeing sterilizing properties.SOLUTION: The cell-separating apparatus 1 is equipped with a decomposition treatment part 2 for separating fat-derived cells from a fat tissue by digesting the fat tissue to afford a cell suspension, a cell-concentrating part 3 for concentrating the cell suspension by centrifugal separation, a transportation route 6 for transporting the cell suspension from the decomposition treatment part 2 to the cell-concentrating part 3, a cell number-counting means 7 installed in the midway position of the transportation route 6, and for counting the number of the cells derived from the fat and contained in the cell suspension, a cell-treating means 8 installed in the midway position of the transportation route 6, and for carrying out the treatment of the cell suspension for increasing the proportion of the fat-derived cells effective for treatment and contained in the cell suspension, and a controlling part 9 for regulating the treatment condition of the fat-derived cells by the cell-treating means 8 based on the number of the fat-derived cells counted by the cell number-counting means 7.

Description

  The present invention relates to a cell separation device.

  Conventionally, an apparatus for separating fat-derived cells including stem cells from adipose tissue collected from a living body is known (see, for example, Patent Document 1).

International Publication No. 2005/012480

  However, the fat-derived cells separated using the apparatus of Patent Document 1 are in a state in which the function is lowered in the process of treatment, or impurities remain. Therefore, there is a problem that it is difficult to obtain the maximum therapeutic effect even if the cell concentrate, which is the final product collected from the apparatus, is used for treatment as it is. It is also conceivable that after the separation, the fat-derived cells are taken out of the apparatus and then subjected to a treatment such as activation or purification. However, there is a possibility that foreign substances are mixed into the fat-derived cells that have been guaranteed sterilization in the apparatus. Furthermore, the fat-derived cells may rather be weakened by the increased number of centrifugation steps required to wash and concentrate the fat-derived cells prior to use in the treatment process, particularly therapy.

  This invention is made | formed in view of the situation mentioned above, Comprising: It aims at providing the cell separation apparatus which can improve the therapeutic effect by a final product, ensuring sterility.

In order to achieve the above object, the present invention provides the following means.
The present invention comprises a digestion treatment unit for producing a cell suspension by separating adipose-derived cells from the adipose tissue by digesting the adipose tissue, a cell concentration unit for concentrating the cell suspension by centrifugation, A transport path for transporting the cell suspension from the decomposition processing section to the cell concentration section, and a cell that is provided at an intermediate position in the transport path and that measures the number of the fat-derived cells contained in the cell suspension A number measuring means and a cell treatment means provided at an intermediate position in the transport path, wherein the cell suspension is subjected to a process for increasing a proportion of fat-derived cells effective for treatment contained in the cell suspension. And a control unit that adjusts processing conditions of the fat-derived cells by the cell processing means based on the number of the fat-derived cells measured by the cell number measuring means.

According to the present invention, the cell suspension produced in the decomposition treatment unit is conveyed to the cell concentration unit via the conveyance path, and concentrated in the cell concentration unit, thereby concentrating the fat-derived cells as the final product. You can get things.
In this case, the cell suspension is a condition in which the number of fat-derived cells contained therein is measured by the number of cells counted during conveyance on the conveyance path, and is appropriately adjusted by the control unit according to the measurement result. Treated by cell treatment means. Thereby, since the cell suspension is conveyed to the cell concentration part in a state where the ratio of effective fat-derived cells is increased, the therapeutic effect of the final product can be improved while ensuring sterility.

In the above invention, the cell processing means includes a cell stimulating unit that stimulates the fat-derived cells in the cell suspension, and the control unit adjusts the magnitude of the stimulation by the cell stimulating unit. May be.
By doing in this way, an appropriate irritation | stimulation can be given to the fat origin cell of the state in which the function fell, and a fat origin cell can be activated.

In the above invention, the cell stimulation unit includes an ultrasonic transducer that generates an ultrasonic wave in the cell suspension, and the control unit outputs an ultrasonic wave generated by the ultrasonic transducer. You may adjust.
Moreover, in the said invention, the said cell stimulation part may be equipped with the light source which irradiates light to the said cell suspension, and the said control part may adjust the intensity | strength of the light irradiated from the said light source.
By doing in this way, a fat-derived cell can be activated with a simple structure and operation.

In the above invention, the cell processing means includes a cell sorting channel for sorting fat-derived cells from the cell suspension, and the control unit allows the cell to flow in the cell sorting channel. The cell density or flow rate of the suspension may be adjusted.
By doing so, it is possible to sort out fat-derived cells out of the components contained in the cell suspension in the sorting channel and transport them to the cell concentrating unit, thereby obtaining a concentrate with higher purity of fat-derived cells. .

Moreover, in the said invention, the said cell selection flow path may hold | maintain the antibody with respect to cells other than the said effective fat origin cell on the surface.
In this way, cells that are not therapeutically effective in the cell sorting channel can be captured by the antibody and the fat-derived cells can be sorted simply by flowing the cell suspension into the cell sorting channel.

  In the above invention, the cell sorting channel is provided at an inlet through which the cell suspension is introduced and at a position away from the inlet along the flow direction of the cell suspension. And a waste liquid side outlet provided at a position obliquely away from the flow direction from the inlet, and the antibody is solidified on the bottom surface thereof. A plurality of linear adsorption layers may be formed substantially parallel to each other at an interval crossing obliquely with respect to the flow direction of the cell suspension.

  In this way, when the cell suspension flows from the inlet into the cell sorting channel, the fat-derived cells move toward the concentration side outlet along the flow direction, and cells other than the fat-derived cells flow. It moves toward the waste liquid side outlet obliquely with respect to the direction. Thereby, fat-derived cells can be selected from the cell suspension and transported to the cell concentration unit.

  Further, in the above invention, an ultrasonic transducer that generates an ultrasonic standing wave in a direction intersecting a flow direction of the cell suspension in the cell sorting channel, and the cell sorting channel in the cell sorting channel, There may be provided a branch channel for diverting the cell suspension at the position of the ultrasonic node to the cell concentrating portion and the cell suspension at the position of the ultrasonic antinode to the waste container.

  By doing in this way, among the cells contained in the cell suspension, fat-derived cells are collected in the abdominal part of the ultrasound and are sent to the cell concentration part, and the red blood cells remaining in the cell suspension are ultrasound. Are collected in the section of the above and are transported to the waste liquid container via the branch flow paths, respectively. As a result, fat-derived cells can be selected from the cell suspension and transported to the cell concentration unit, and the fat-derived cells are activated by the irradiation of ultrasonic waves, so that the therapeutic effect of the final product can be improved. It can be further increased.

  According to the present invention, it is possible to improve the therapeutic effect of the final product while ensuring sterility.

1 is an overall configuration diagram of a cell separation device according to a first embodiment of the present invention. It is a figure which shows the structure of the cell number measurement means of the cell separation apparatus of FIG. It is a figure which shows the structure of the cell stimulation part of the cell separation apparatus of FIG. It is a figure which shows the modification of the cell number measurement means of FIG. It is a figure which shows the modification of the cell separation apparatus of FIG. It is a whole block diagram of the cell separation apparatus which concerns on the 2nd Embodiment of this invention. It is a figure which shows the modification of the cell selection flow path of the cell separation apparatus of FIG. It is a figure explaining the sorting method of the fat origin cell by the cell sorting channel of Drawing 7. It is a figure which shows another modification of the cell selection flow path of the cell separation apparatus of FIG.

Below, cell separation device 1 concerning a 1st embodiment of the present invention is explained with reference to drawings.
As shown in FIG. 1, the cell separation device 1 according to this embodiment includes a decomposition processing unit 2 that generates a cell suspension by digesting adipose tissue, and cells that concentrate the cell suspension by centrifugation. Concentration unit 3, decomposition processing unit 2, cell concentration unit 3, cleaning liquid bag (waste liquid container) 4, and transport path 6 that connects waste liquid bag 5, and a cell number measuring section (in the middle of transport path 6) A cell number measuring unit) 7 and a cell stimulating unit (cell processing unit) 8; and a control unit 9 for controlling operations of the units 2, 3, 7, and 8.

  The degradation processing unit 2 agitates the adipose tissue collected by suction from the living body and the digestive enzyme solution that degrades the adipose tissue in the collection container 10 using an agitator (not shown). As a result, adipose-derived cells bound to the adipose tissue are separated from the adipose tissue, and a cell suspension in which the adipose-derived cells are suspended in the digestive enzyme solution is generated. The bottom surface 10a of the collection container 10 is inclined in one direction, and the cell suspension is discharged from the lowest position of the bottom surface 10a. The code | symbol 10b is a filter which prevents that comparatively big impurities, such as the residue of digested adipose tissue, are discharged | emitted with a cell suspension.

  The cell concentration unit 3 includes a centrifuge 11 in which a centrifuge container 12 is attached to a bucket 11a, and centrifuges a cell suspension conveyed from the decomposition processing unit 2 via a conveyance path 6. A pipe 13 is inserted in the centrifuge container 10 up to a midpoint in the depth direction, and a cell suspension or a washing solution is supplied into the centrifuge container 12 through the pipe 13 and is centrifuged. Later supernatant is drained. As a result, when the supernatant is discharged after centrifugation of the cell suspension, the supernatant is discharged leaving a certain amount of supernatant and a precipitate of fat-derived cells in the centrifuge container 12, and the supernatant The cell-derived cells that have been precipitated by the momentum of the inflow of the washing liquid when the washing liquid is supplied after the discharge of the liquid are stirred in the washing liquid.

  The transport path 6 includes a tube 14 that connects the collection container 10, the centrifuge container 12, the cleaning liquid bag 4, and the waste liquid bag 5, and valves V <b> 1 to V <b> 10 and a liquid feed pump 15 that are provided in the middle of the tube 14. ing. Reference numeral 6 a indicates a joint that branches the tube 14. For example, an LR solution (Lactated Ringer's Buffer) is accommodated in the cleaning solution bag 4 as a cleaning solution.

  The cell number counting unit 7 is provided at a midpoint of the tube 14 in which the cell suspension is transported from the collection container 10 to the centrifuge container 12, and is opposed to the tube 14 in the radial direction as shown in FIG. A laser light source 16 and a detector 17 are provided. The laser light L emitted from the laser light source 16 is scattered by the fat-derived cells A contained in the cell suspension in the tube 14 when passing through the tube 14. The intensity of the scattered light generated at this time is proportional to the number of fat-derived cells A.

The detector 17 detects the scattered light of the laser light L, and calculates the number of fat-derived cells A in the cell suspension based on the detected intensity of the scattered light.
Note that a part of the tube 14 may be formed of a glass tube 14a or the like at a position sandwiched between the laser light source 16 and the detector 17 so that the scattered light can be accurately detected by the detector 17.

  The cell stimulating unit 8 is provided at an intermediate position of the tube 14 in which the cell suspension is conveyed from the collection container 10 to the centrifuge container 12. As shown in FIG. 3, the cell stimulating unit 8 includes an ultrasonic transducer 18 that generates ultrasonic vibrations in the tube 14. A part of the tube 14 is covered with a metal pipe 19 so that the ultrasonic wave generated from the ultrasonic transducer 18 is efficiently transmitted to the fibrillation suspension in the tube 14 by the metal pipe 19. It has become.

Various parameters are set so that the ultrasonic wave generated by the ultrasonic transducer 18 in the cell suspension does not excessively affect the fat-derived cell A while stimulating the fat-derived cell A. For example, the ultrasonic wave output from the ultrasonic transducer 18 preferably has a frequency of 1.5 MHz, a burst width of 200 μsec, a repetition period of 1 kHz, an effective ultrasonic wave irradiation area of 3.88 cm ² , and an output of about 117 mW. Thereby, the fat origin cell A in a cell suspension is moderately stimulated with an ultrasonic wave, and the function of the fat origin cell which had been reduced until then is activated.

  The control unit 9 is connected to the decomposition processing unit 2, the cell concentration unit 3, the valves V1 to V10, and the liquid feeding pump 15 through a wiring (not shown), and controls these operations according to a preset processing procedure. It has become. Further, the control unit 9 causes the laser light source 16 to emit the laser light L when the conveyance of the cell suspension from the decomposition processing unit 2 to the cell concentration unit 3 is started. Then, the control unit 9 receives information on the number of fat-derived cells calculated by the detector 17 from the detector 17 and adjusts the output of the ultrasonic transducer 18 so as to be proportional to the number of fat-derived cells. It has become.

  Note that the installation positions of the cell number measurement unit 7 and the cell stimulation unit 8 are not limited to the positions illustrated in FIG. 1, but the cell number measurement unit 7 may be installed in front of the cell stimulation unit 8. preferable. Thereby, the measurement result by the cell number measurement part 7 can be more effectively reflected in the control of the cell stimulation part 8 by the control part 9. Furthermore, the cell stimulating unit 7 is preferably installed immediately before the cell concentrating unit 3. By doing in this way, the conveyance time of the cell suspension from the cell stimulation part 8 to the cell concentration part 3 is shortened, and the activity of the fat origin cell at the time of the completion of the process in the cell concentration part 3 is maintained in a higher state. be able to.

The operation of the cell separation device 1 configured as described above will be described below.
The cell separation device 1 according to the present embodiment stirs the adipose tissue together with the digestive enzyme solution in the collection container 10 to decompose the adipose tissue, and then transports the generated cell suspension toward the centrifuge container 12. At the same time, emission of laser light L from the laser light source 16 and output of ultrasonic waves from the ultrasonic transducer 18 are started.

  After the transfer of the cell suspension is completed, the cell separation device 1 centrifuges the cell suspension in the cell concentration unit 3 and discharges the supernatant from the centrifuge container 12 to the waste liquid bag 5. Next, the cell separation device 1 carries the washing solution from the washing solution bag 4 to the centrifuge container 12, centrifuges, and discharges the supernatant from the centrifuge vessel 12 to wash the fat-derived cells. Washing may be performed a plurality of times as necessary. Finally, a concentrate of fat-derived cells is obtained as a final product at the bottom of the centrifuge container 12.

  In this case, according to the present embodiment, the fat-derived cells are activated by being irradiated with ultrasonic waves during conveyance to the centrifuge container 12. Thereby, even if the final product containing abundant adipose-derived cells effective in the treatment of the activated state is collected from the centrifuge container 12, even if the final product is used for the treatment as it is, a high therapeutic effect is obtained. Obtainable. In particular, by adjusting the intensity of stimulation by ultrasound according to the number of fat-derived cells, it is possible to effectively activate fat-derived cells by giving the fat-derived cells stimulation of an appropriate size. There is.

  Further, even if the cell stimulating unit 7 is provided in this way, the total processing time and processing steps, in particular, the number of centrifugations do not increase, so that fat-derived cells can be recovered in a healthy state. There are advantages. Furthermore, since stimulation is given to fat-derived cells in the tube 14 without contact, there is an advantage that the sterility of the fat-derived cells can be ensured.

  In the above embodiment, the cell number measurement unit 7 measures the number of fat-derived cells based on the scattered light of the laser light L. Instead, as shown in FIG. 14 may include an imaging unit that captures an image of the inside 14, for example, a CCD (charge-coupled device) camera 20, and the number of fat-derived cells in the image captured by the CCD camera 20 may be measured.

  For example, an image captured by the CCD camera 20 is input to the control unit 9, and the control unit 9 processes the input image to measure the number of fat-derived cells present in the image. Yes. At this time, a window 14b made of a highly transparent material such as glass may be provided in a part of the tube 14 so that a clear image of fat-derived cells can be taken by the CCD camera 20. Even in this way, the number of fat-derived cells can be measured without contacting the fat-derived cells.

The number of fat-derived cells may be measured using a cell counter or a fluorescent reagent that emits fluorescence due to the activity of living cells.
When the cell counter is used, for example, a sample port (not shown) for collecting a part of the cell suspension is provided at an intermediate position of the tube 14, and the cell suspension is being transferred from the collection container 10 to the centrifuge container 12. Collect a small amount of liquid. Then, the operator uses a cell counter to count the number of fat-derived cells in the cell suspension under a microscope.

In the case of using a fluorescent reagent, for example, a cell suspension in which the fluorescent reagent is added to the collection container 10 during the processing in the decomposition processing unit 2 and flows in the tube 14 while being transported to the centrifuge container 12. Is measured with a fluorometer (not shown). The measured fluorescence intensity is proportional to the number of fat-derived cells.
Even in this way, the number of fat-derived cells can be measured while maintaining the sterility of the fat-derived cells.

Moreover, in the said embodiment, although it decided to irradiate the ultrasonic wave to the cell suspension currently flowing through the inside of the tube 14, it replaces with this and is discharged | emitted from the collection container 10, as FIG. 5 shows. A processing container 21 for temporarily storing the cell suspension thus prepared may be provided, and the cell suspension stored in the processing container 21 may be irradiated with ultrasonic waves.
By doing in this way, many fat origin cells can be collectively activated by one time of ultrasonic irradiation.

Moreover, in the said embodiment, it replaces with the ultrasonic transducer | vibrator 18 and a light source (cell processing means) may be provided, and a fat-derived cell may be activated by irradiating the tube 14 with light, for example, infrared rays and ultraviolet rays. .
The light is applied to the cell suspension flowing in the tube 14 with a sufficiently low output so as not to damage the fat-derived cells. The control unit 9 adjusts the intensity of the irradiated light so as to be proportional to the number of fat-derived cells measured by the cell number measuring unit 7. Even if it does in this way, a fat origin cell can be activated only by a simple structure and operation which is non-contact with a fat origin cell.

Next, a cell separation device 1 according to a second embodiment of the present invention will be described with reference to FIGS.
In the present embodiment, the configuration different from that of the first embodiment will be mainly described, and the configuration common to the first embodiment will be denoted by the same reference numerals and description thereof will be omitted.
As shown in FIG. 6, the cell separation device 1 according to the present embodiment is different from the first embodiment in that a cell sorting channel (cell processing means) 22 is provided instead of the cell stimulating unit 8.

  The cell sorting channel 22 is configured by solidifying an antibody that specifically binds to cells such as red blood cells other than fat-derived cells, for example, a CD34 antibody, on the inner surface of a pipe whose both ends are connected to the tube 14. Has been. For example, the pipe is spirally wound, so that a long dimension is ensured while keeping an installation area small. As a result, the contact area between the cell suspension and the antibody can be increased, and undesired cells other than fat-derived cells can be more reliably captured in the cell sorting channel 22.

  The control unit 9 adjusts the flow rate of the cell suspension with the liquid feeding pump 15 so as to be inversely proportional to the number of fat-derived cells measured by the cell number measuring unit 7. Alternatively, when the number of fat-derived cells measured by the cell number measuring unit 7 is greater than a predetermined threshold, the control unit 9 causes the washing liquid to flow from the washing liquid bag 4 into the tube 14 while adjusting the flow rate, thereby suspending the cell. The solution is diluted so that the density of fat-derived cells is kept below a predetermined threshold.

The operation of the cell separation device 1 configured as described above will be described below.
The cell separation device 1 according to this embodiment conveys the cell suspension generated in the collection container 10 from the collection container 10 to the centrifuge container 12. At this time, fat-derived cells are sorted from the cell suspension in the cell sorting channel 22 and conveyed to the centrifuge container 12.

  Thus, according to the present embodiment, since the cell suspension from which impurities such as red blood cells have been removed is conveyed into the centrifuge container 12, a final product with high purity of fat-derived cells effective for treatment is obtained. can get. As a result, when the collected final product is used for treatment as it is, the therapeutic effect of fat-derived cells can be sufficiently exerted without the function of fat-derived cells being inhibited by impurities such as red blood cells. There is an advantage that can be improved. Moreover, since the closed system of the whole cell separation apparatus 1 is maintained even if the cell sorting channel 22 is provided in the middle of the tube 14 in this way, there is an advantage that the sterility of the final product can be ensured. is there.

  In the above-described embodiment, the cell sorting channel 22 is configured such that the antibody is solidified on the inner surface of the pipe. Instead, as shown in FIG. 7, as the cell sorting channel 22, a linear adsorption layer 24 in which the antibody is solidified is formed on the bottom surface of the separation container 23 arranged in the middle of the tube 14. Alternatively, a configuration may be adopted in which the cell suspension is inclined with respect to the flow direction of the cell suspension and spaced substantially in parallel. The adsorption layer 24 is formed, for example, inclined by 45 ° with respect to the flow direction.

  In the separation container 23, the fat-derived cells A out of the cells in the cell suspension that has been transported from the collection container 10 and flowed in from the inlet 23a are approximately in accordance with the flow direction of the cell suspension, as shown in FIG. It flows straight and is discharged from a concentrating section side outlet 23b provided substantially in front of the inlet 23a. On the other hand, the cells B other than the fat-derived cells flow obliquely in the separation container 23 along the inclination of the adsorption layer 24 and are discharged from a waste liquid side outlet 23c provided diagonally to the inlet 23a. In order to separate cells discharged from the outlets 23b and 23c with higher accuracy, a partition 25 may be provided between the outlets 23b and 23c.

At this time, so that the cells A and B in the cell suspension flow along the bottom surface of the separation container 23 and the cells B other than the fat-derived cells flow without staying in the adsorption layer 24, The flow rate of the cell suspension is adjusted. Even in this case, since the fat-derived cells A are selected from the cell group contained in the cell suspension and transported to the centrifuge container 12, A final product with high purity can be obtained.

In the above embodiment, as shown in FIG. 9, fat-derived cells may be selected from the cell suspension using ultrasonic waves in the cell selection flow path 22.
As shown by a broken line in FIG. 9, cell selection is performed from the ultrasonic transducer 26 while adjusting the frequency so that a standing wave is formed in a direction intersecting the flow direction of the cell suspension, preferably perpendicularly. Ultrasound is generated in the cell suspension in the channel 22. As a result, fat-derived cells A having negative acoustic characteristics are collected in the ultrasonic antinode and red blood cells C having positive acoustic characteristics are collected in the ultrasonic node.

  Therefore, the branch flow path 27 is arranged so that the fat-derived cells A collected in the ultrasonic abdomen are diverted to the centrifuge container 12, and the red blood cells C collected in the ultrasonic nodes are diverted to the waste bag 5. , The fat-derived cells A can be selected from the cell suspension and transported to the centrifuge container 12. In addition, since the fat-derived cells A are activated by ultrasonic waves in the cell sorting channel 22, the therapeutic effect of the final product can be further enhanced.

Moreover, in the said embodiment, you may provide the cell stimulation part 8 in 1st Embodiment mentioned above.
In this way, the therapeutic effect of the final product can be maximized.

DESCRIPTION OF SYMBOLS 1 Cell separator 2 Decomposition processing part 3 Cell concentration part 4 Washing liquid bag 5 Waste liquid bag (waste liquid container)
6 Transport route 7 Cell count unit (cell count measuring means)
8 Cell stimulation part (cell treatment means)
DESCRIPTION OF SYMBOLS 9 Control part 10 Collection container 11 Centrifuge 11a Bucket 12 Centrifugal container 13 Piping 14 Tube 14a Glass tube 14b Window 15 Liquid feed pump 16 Laser light source (cell number measurement means)
17 Detector (Cell count measuring means)
18 Ultrasonic transducer (cell stimulation part)
19 Metal pipe (cell stimulation part)
20 CCD camera (cell number counting means)
21 Processing Container 22 Cell Sorting Channel 23 Separation Container 23a Inlet 23b Concentration Unit Outlet 23c Waste Liquid Side Outlet 24 Adsorption Layer 25 Partition 26 Ultrasonic Vibrator (Cell Sorting Means, Cell Stimulation Unit)
27 Branch channel A Adipose-derived cell B Non-adipose-derived cell C Red blood cell

Claims (8)

A digestion processing unit that separates adipose-derived cells from the adipose tissue by digesting the adipose tissue to generate a cell suspension;
A cell concentration part for concentrating the cell suspension by centrifugation;
A transport path for transporting the cell suspension from the decomposition processing section to the cell concentration section;
A cell number measuring means provided at an intermediate position of the transport path, for measuring the number of the fat-derived cells contained in the cell suspension;
A cell treatment means that is provided at an intermediate position in the transport path, and performs a treatment for increasing the proportion of adipose-derived cells effective in the treatment contained in the cell suspension with respect to the cell suspension;
A cell separation apparatus comprising: a control unit that adjusts processing conditions of the fat-derived cells by the cell processing means based on the number of the fat-derived cells measured by the cell number measuring means. The cell processing means comprises a cell stimulating unit for stimulating the fat-derived cells,
The cell separation device according to claim 1, wherein the control unit adjusts the magnitude of the stimulation by the cell stimulation unit. The cell stimulation unit includes an ultrasonic transducer that generates ultrasonic waves in the cell suspension,
The cell separation device according to claim 2, wherein the control unit adjusts an output of ultrasonic waves from the ultrasonic transducer. The cell stimulation unit includes a light source that irradiates light to the cell suspension,
The cell separation device according to claim 2, wherein the control unit adjusts the intensity of light emitted from the light source. The cell processing means comprises a cell sorting channel for sorting fat-derived cells from the cell suspension;
The cell separation device according to claim 1, wherein the control unit adjusts a cell density or a flow rate of the cell suspension that is allowed to flow in the cell sorting channel.   The cell separation device according to claim 5, wherein the cell sorting channel holds antibodies against cells other than the effective fat-derived cells on the surface thereof.   The cell sorting channel includes an inlet through which the cell suspension is introduced, and a concentration unit side provided at a position away from the inlet along the flow direction of the cell suspension and connected to the cell concentration unit A plurality of linear adsorbent layers, each comprising an outlet and a waste liquid side outlet provided at a position away from the inlet in an oblique direction with respect to the flow direction, wherein the antibody is solidified on the bottom. The cell separation device according to claim 6, wherein the cell separation device is formed so as to cross obliquely with respect to the flow direction of the cell suspension and to be substantially parallel with an interval. An ultrasonic transducer that generates an ultrasonic standing wave in a direction intersecting a flow direction of the cell suspension in the cell sorting channel;
A branch flow path for diverting the cell suspension at the position of the ultrasonic node in the cell sorting flow path to the cell concentration unit and the cell suspension at the position of the ultrasonic antinode to a waste container; The cell separation device according to claim 5 provided.

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