JP2011172545A - Cell separation apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cell separation apparatus which recovers cells in an activated state and separates fat-derived cells containing stem cells, etc. from fat tissues while guaranteeing the sterilization. <P>SOLUTION: The cell separation apparatus 1 includes: a decomposition treatment unit 2 for digesting a living tissue for producing a cell suspension in which the cells are isolated from the living tissue; a cell concentrating unit 3 for concentrating the produced cell suspension at the decomposition treatment unit 2; a storage container 8 for storing the cell suspension; a conveying path 6 for being connected to the decomposition treatment unit 2, the cell concentrating unit 3 and the storage container 8 to convey the cell suspension; a state amount measuring section 11 for measuring a state amount showing the state of the cell suspension stored in the storage container 8; a cell stimulator 10 for stimulating the cells contained in the cell suspension stored in the storage container 8; and a control section 7 for controlling the degree of stimulation applied to the cells by the cell stimulator 10 based on the measured state amount measured by the state amount measuring section 11. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a cell separation device.

  Conventionally, a cell separation device that separates adipose-derived cells including stem cells from adipose tissue collected from a living body is known (see, for example, Patent Document 1). The separated fat-derived cells are used for treatment, for example, by being transplanted into the body.

JP 2009-136168 A

  Adipose-derived cells that have been removed from the body and subjected to various treatments are collected in a state of reduced function, and it is difficult to obtain a sufficient therapeutic effect even if they are used in treatment in this state. Therefore, it is desirable to stimulate and activate fat-derived cells before use in therapy. However, the operation for activating cells is complicated, and there is a problem that it may cause problems such as contamination with germs.

  This invention is made | formed in view of the situation mentioned above, Comprising: It aims at providing the cell separation apparatus which can collect | recover a cell in the activated state, ensuring sterility.

In order to achieve the above object, the present invention provides the following means.
The present invention includes a decomposition processing unit that generates a cell suspension in which a biological tissue is digested to release cells from the biological tissue, and a cell concentration unit that concentrates the cell suspension generated in the decomposition processing unit. A storage container for storing the cell suspension; a decomposition path; a cell concentration section; and a storage path for transporting the cell suspension, connected to the storage container, and stored in the storage container. A state quantity measuring unit for measuring a state quantity indicating a state of the cell suspension, a cell stimulating means for stimulating cells contained in the cell suspension accommodated in the container, and the state quantity measuring unit. There is provided a cell separation device comprising: a control unit that controls the magnitude of stimulation given to the cell by the cell stimulation means based on the measured state quantity.

According to the present invention, the cell suspension generated by digesting the biological tissue in the decomposition processing unit is transported to the cell concentration unit via the transport path and concentrated, so that the cells separated from the biological tissue are concentrated. A concentrate can be obtained.
In this case, when the cell suspension is once stored in the storage container during the processing, the state quantity at that time is measured by the state quantity measuring unit. Then, a stimulus of a size appropriately controlled by the control unit is given to the cells in the cell suspension by the cell stimulating means. Thereby, cells can be activated while ensuring sterility in a closed system, and activated cells can be obtained.

In the above invention, the state quantity measuring unit is a temperature sensor that measures the temperature of the cell suspension in the storage container, and the cell stimulation means includes a heater that heats the storage container, The control unit may control the temperature of the heater based on the temperature of the cell suspension measured by the temperature sensor.
By doing in this way, a cell can be activated with a heat | fever.

Moreover, in the said invention, it is good also as providing the stirring means which stirs the inside of the said storage container.
In this way, by heating the cell suspension while stirring, the temperature at each position of the cell suspension can be made uniform, and each cell can be activated evenly.

Moreover, in the said invention, the said state quantity measurement part is a liquid quantity sensor which measures the liquid quantity of the said cell suspension in the said storage container, The said cell stimulation means transmits an ultrasonic wave in the said storage container. An ultrasonic vibrator to be generated, and the control unit controls the output of the ultrasonic wave generated by the ultrasonic vibrator based on the liquid volume of the cell suspension measured by the liquid volume sensor. It is good.
By doing in this way, a cell can be activated with an ultrasonic wave.

Further, in the above invention, the state quantity measuring unit is an oxygen concentration sensor that measures an oxygen concentration of the cell suspension stored in the storage container, and the cell stimulation means includes air in the storage container. A low oxygen gas supply unit that supplies a gas having a lower oxygen concentration is provided, and the control unit supplies the low oxygen gas supply unit into the storage container based on the oxygen concentration measured by the oxygen concentration sensor. The supply amount of the gas may be controlled.
By doing in this way, the oxygen concentration of a cell suspension can be reduced and a cell can be activated.

Further, in the above invention, the state quantity measuring unit is a liquid quantity sensor for measuring a liquid quantity of the cell suspension stored in the storage container, and the cell stimulation means is provided in the storage container. An activator supply unit that supplies an activator for activating cells is provided, and the control unit is placed in the container from the activator supply unit based on the liquid amount measured by the liquid amount sensor. It is good also as controlling the supply amount of the said activator to supply.
By doing in this way, a cell can be activated with an activator.

Moreover, in the said invention, it is preferable to provide the activator removal means which removes the said activator.
By doing in this way, after activating a cell, an unnecessary activation agent can be removed.
Moreover, in the said invention, the said activator is good also as being a growth factor or a nitric oxide production factor.
By doing in this way, a cell can be activated efficiently.

Moreover, in the above invention, the state quantity measuring unit is a cell density meter that measures the density of the cells contained in the cell suspension in the container, and the cell stimulating means is in the container. In this case, the cells contained in the cell suspension are brought into contact with each other to stimulate the cells. The cell separation apparatus according to claim 1, wherein a supply amount of the cell suspension supplied into the cell suspension is controlled.
By doing in this way, it can activate by making a cell mutually contact on suitable conditions.

Moreover, in the said invention, the said cell stimulating means is good also as aggregating the said cell because the inner surface of the said storage container is coat | covered with the non-adhesive material to which the said cell does not adhere | attach.
The cell stimulation means may aggregate the cells in the groove by forming a groove on the bottom surface of the container.
In this way, cells can be easily and efficiently aggregated and brought into contact with each other.

  According to the present invention, there is an effect that activated cells can be obtained while ensuring sterility.

1 is an overall configuration diagram of a cell separation device according to a first embodiment of the present invention. It is a graph which shows the relationship between temperature and the number of activated fat origin cells. It is a graph which shows the relationship between heating time and the number of activated fat-derived cells. It is a modification of the cell separator of FIG. 1, and is a figure which shows a structure when activating fat origin cells by an ultrasonic wave. It is another modification of the cell separation apparatus of FIG. 1, and is a figure which shows a structure when activating fat origin cells by making a cell suspension into a hypoxic state. It is a whole block diagram of the cell separation apparatus which concerns on the 2nd Embodiment of this invention. It is a figure which shows the modification of a cell stimulation container. It is a whole block diagram of the cell separation apparatus which concerns on the 3rd Embodiment of this invention.

A first embodiment of the present invention will be described below with reference to FIGS.
As shown in FIG. 1, the cell separation device 1 according to this embodiment includes a decomposition processing unit 2 that generates a cell suspension of fat-derived cells (cells) by digesting adipose tissue (biological tissue); A cell concentration unit 3 for washing and concentrating fat-derived cells by centrifugation, a cleaning solution bag 4 for storing the cleaning solution, a waste solution bag 5 for discharging the waste solution generated in the decomposition processing unit 2 and the cell concentration unit 3, and a decomposition treatment A transport path 6 for transporting the solution between the section 2, the cell concentration section 3 and each of the bags 4 and 5, and a computer (control section) 7 for controlling the operation of the decomposition processing section 2, the cell concentration section 3 and the transport path 6 It has.

  The decomposition processing unit 2 includes a collection container (container container) 8 for collecting adipose tissue, a shaker (stirring means) 9 for shaking the collection container 8, and a heater (cell stimulation) for heating the collection container 8 Means) 10 and a temperature sensor (state quantity measuring unit) 11 for measuring the temperature in the collection container 8.

  The collection container 8 has a cannula (not shown) connected to an introduction port 8a provided in the upper part, and collects adipose tissue sucked from the human body through the cannula. The bottom surface 8b of the collection container 8 is inclined in one direction, and a discharge port is provided at the lowest position. The outlet is covered with a filter 8c that captures adipose tissue and allows fat-derived cells to pass through. In addition, the digestion enzyme solution is supplied to the collection container 8 from the upper enzyme supply port 8d using a syringe or the like by an operation by the operator.

The shaker 9 has a cylindrical base 9a with an open top, and the contents of the collection container 8 are agitated by storing the collection container 8 in the base 9a and rotating the base 9a in translation. .
The heater 10 has, for example, a sheet shape, and is provided on the inner surface of the base 9a.
The temperature sensor 11 is an infrared type or a thermocouple. The temperature sensor 11 is installed in a window penetrating the base 9a. In the case of the infrared type, the temperature sensor 11 is arranged with a gap between the side wall of the collection container 8 and in the case of a thermocouple, the collection container 8 is disposed. It is arranged in contact with the side wall of.

  The adipose tissue collected in the collection container 8 is digested by being agitated by the shaker 9 together with the digestive enzyme solution. As a result, the adipose-derived cells bound to the adipose tissue are released into the digestive enzyme solution and a cell suspension is generated. In addition, the generated cell suspension is separated from the adipose tissue residue and stored in the lower layer by allowing the collection container 8 to stand after agitation, and the cell suspension is selected when discharged from the outlet in this state. Thus, it is discharged from the collection container 8.

The cell concentrating unit 3 includes a pair of centrifuge containers 12 and a centrifuge (activator removing means) 13 that rotates the centrifuge container 12 with its bottom portion directed radially outward.
The centrifuge container 12 accommodates the cell suspension discharged from the collection container 8 via the transport path 6. The centrifuge container 12 is inserted with the pipe 12a to a midpoint in the depth direction, and when the inside of the pipe 12a is sucked by the transport path 6, the solution in the centrifuge container 12 is discharged leaving a predetermined amount. At this time, if the cell suspension is centrifuged and then discharged through the pipe 12a, the supernatant is selectively discharged while leaving the precipitated fat-derived cells at the bottom.

The cleaning liquid bag 4 contains, for example, physiological saline or lactated Ringen buffer as a cleaning liquid.
The conveyance path 6 is arranged at a midway position of the tube 15 connecting the containers 8 and 12 and the bags 4 and 5 via the joint 14 and the tube 15, and feeds the liquid in the tube 15 in a non-contact manner. A liquid feed pump 16 and valves V <b> 1 to V <b> 10 for switching a liquid feed path by the pump 16 are provided.

  The computer 7 includes a storage unit and a CPU (central processing unit), and a program created in accordance with a fat tissue processing procedure is stored in the storage unit. The computer 7 is connected to a shaker 9, a heater 10, a temperature sensor 11, a centrifuge 13, a pump 16, and the valves V1 to V10 via wiring (not shown), and operates these according to a program. Yes.

  At this time, when digesting adipose tissue in the collection container 8, the computer 7 controls the temperature of the heater 10 so that the temperature measured by the temperature sensor 11 is maintained at 37 ° C. 9, the inside of the collection container 8 is stirred for a predetermined time. Subsequently, the computer 7 raises the temperature of the heater 11 and controls the temperature of the heater 11 so that the temperature measured by the temperature sensor 10 is maintained at 38 to 42 ° C., more preferably 40 ° C. The collection container 8 is agitated by the shaker 9 for about 30 minutes, preferably about 30 minutes. At this time, if the temperature measured by the temperature sensor 10, that is, the temperature of the cell suspension exceeds 42 ° C., the function of the fat-derived cells may be lost due to aggregation of proteins in the fat-derived cells. This is not preferable.

The operation and action of the cell separation device 1 configured as described above will be described below.
When the adipose tissue is collected in the collection container 8 and the digestive enzyme solution is supplied by the operator, the cell separation device 1 according to the present embodiment stirs the adipose tissue and the digestive enzyme solution for a predetermined time. A turbid liquid is produced. Subsequently, the cell separation device 1 raises the temperature of the cell suspension from 37 ° C., and further keeps stirring the cell suspension while maintaining the temperature at 38 to 42 ° C. Then, the collection container 8 is allowed to stand still. After that, the cell suspension is conveyed to the centrifuge container 12. Next, the cell separation device 1 sediments the fat-derived cells by centrifuging the cell suspension, and after discharging the supernatant via the pipe 12a, the washing liquid is supplied, centrifuged, and the supernatant is discharged. Repeat several times. As a result, the digestive enzyme solution contained in the cell suspension is removed, and a highly pure fat-derived cell concentrate is obtained in the centrifuge container 12.

  In this case, according to the present embodiment, by further increasing the temperature of the cell suspension from 37 ° C. after digestion of the adipose tissue, the fat-derived cells in the cell suspension whose function has been reduced so far can be obtained. Irritate. As a result, the fat-derived cells are activated and become active, and there is an advantage that a sufficient therapeutic effect can be obtained quickly even if the cells are recovered and used as they are. In addition, non-contact stimulation of fat-derived cells using heat can activate fat-derived cells with a simple configuration and operation even in a closed system, and sterilization of fat-derived cells. There is an advantage that can be secured.

In FIG. 2, the result of having measured the state of the activity of the fat origin cell when changing temperature is shown.
First, adipose-derived cells separated from human adipose tissue were heat-treated by incubating at a predetermined temperature for 30 minutes. Next, using a BD BioCoat Matrigel invasion chamber manufactured by Nippon Becton Dickison, the number of adipose-derived cells (hereinafter referred to as migratory cells) that migrated in the chamber was counted. Specifically, 10% FBS medium supplemented with 100 ng / ml SDF-1 was added to the well, heat-treated fat-derived cells were placed in the cell culture insert, cultured for 24 hours, and then transferred from the cell culture insert to the well. The number of migrated cells was counted.

  The above experiment was performed in the case of heat treatment at 37, 38, 40, 42, and 43 ° C. using a cell suspension of adipose-derived cells prepared to approximately the same number of cells. Thereby, the number of fat-derived cells having sufficiently high activity that were attracted and moved by SDF-1 and FBS was measured, and the effect of activation by heat treatment was evaluated. FIG. 2 shows the number of migrating cells measured at each temperature converted into a ratio when the number of migratory cells measured at 37 ° C. is 1. FIG. 2 shows that the number of migratory cells increases from 37 ° C. with increasing temperature, decreases after reaching the maximum at 40 ° C., and falls below the value at 37 ° C. at 43 ° C. That is, it was confirmed that human adipose-derived cells are most active at 40 ° C.

  FIG. 3 shows the measurement results of the relationship between the heating time and the number of migrating cells when a cell suspension of adipose-derived cells separated from human adipose tissue is heated to 40 ° C. The number of migrating cells was measured in the same manner as the experiment shown in FIG. From FIG. 3, it was confirmed that the number of migrating cells increased with time after application of heat at 40 ° C., and the activity was sufficiently enhanced when 30 minutes passed.

  In the above-described embodiment, the fat-derived cells are activated in the collection container 8, but instead, the fat-derived cells may be activated in the centrifuge container (accommodating container) 12. In this case, a heater and a temperature sensor (not shown) are separately provided in the holder 13a. By doing so, the time from activation of fat-derived cells to use for treatment is shortened, and fat-derived cells can be used for treatment in a state where the activity is maintained at a higher level.

Moreover, in the said embodiment, although it decided to activate by heating a fat origin cell, it may replace with this and may activate by irradiating an ultrasonic wave.
In this case, as shown in FIG. 4, an ultrasonic transducer (cell stimulating means) 17 is provided near the side surface of the collection container 8, and high frequency power is supplied to the ultrasonic transducer 17 from an ultrasonic generator (not shown). As a result, ultrasonic waves are generated in the collection container 8.

  In this case, an interface sensor (state quantity measuring unit) 18 for detecting the interface of the cell suspension in the collection container 8 is provided. The interface sensor 18 emits the laser light L in a substantially horizontal direction at each position in the depth direction of the collection container 8, and the laser light L that has passed through the collection container 8 at each position in the depth direction of the collection container 8. Receive light. Thereby, the interface sensor 18 detects the interface between the cell suspension and the residue of the fatty tissue from the difference in the intensity of the laser light L received at each position.

When the digestion of the adipose tissue is completed in the collection container 8, the computer 7 waits for a predetermined time and separates the cell suspension and the adipose tissue residue in the collection container 8, and then the cell suspension is performed by the interface sensor 18. The depth position of the interface between the fluid and the adipose tissue residue is detected. Then, the computer 7 sets the output of the ultrasonic wave supplied from the ultrasonic generator to the ultrasonic transducer 17 based on the detected depth position of the interface, and generates an ultrasonic wave in the collection container 8.
Even in this way, fat-derived cells can be activated in a non-contact manner. In addition, by controlling the output of the ultrasonic wave generated according to the amount of the cell suspension, it is possible to give a suitable amount of stimulation to the fat-derived cells.

The interface sensor 18 may be an ultrasonic sensor or a magnetic sensor. In this case, if there is a residue of adipose tissue in the upper layer of the cell suspension, the amount of the cell suspension is not correctly measured. Therefore, the cells in the centrifuge container 12 or in a separate container (not shown) It is preferable to generate ultrasonic waves by detecting the liquid level of the suspension.
Further, in place of the interface sensor 18, a weight sensor (not shown, state quantity measuring unit) for measuring the weight of the collection container 8 is provided, and the computer 7 determines the weight of the contents of the collection container 8 measured by the weight sensor. Accordingly, the output of the ultrasonic wave may be controlled.

Moreover, in the said embodiment, it is good also as activating a fat origin cell by making the surrounding environment of a fat origin cell low oxygen.
In this case, as shown in FIG. 5, a dissolved oxygen meter (state quantity measuring unit, oxygen concentration sensor) 19 is provided in the collection container 8 and connected to a gas cylinder (cell stimulation means, low oxygen gas supply unit) 20. The pipe 20a is inserted to the vicinity of the bottom in the collection container 8 through the valve V11. The gas in the gas cylinder 20 is preferably an inert gas such as pure nitrogen that can effectively dilute oxygen in the cell suspension while suppressing the influence on fat-derived cells. The computer 7 supplies the gas into the cell suspension by opening the valve V11 until the dissolved oxygen concentration measured by the dissolved oxygen meter 19 decreases to a predetermined value. An air filter 21 for removing dust in the gas may be provided in the middle of the pipe 20a.

Alternatively, a gas permeable material is used as the material of the cleaning liquid bag 4 and the cleaning liquid bag 4 is placed in a sealed container (not shown). And it is good also as supplying the washing | cleaning liquid which reduced the amount of dissolved oxygen to the cell suspension by making the inside of a container into a low oxygen atmosphere.
Even in this case, the fat-derived cells can be activated while maintaining the sterility of the fat-derived cells with a simple operation and configuration.

Next, a second embodiment of the present invention will be described below with reference to FIGS.
In the present embodiment, the same components as those in the first embodiment are denoted by the same reference numerals, description thereof is omitted, and differences from the first embodiment are mainly described.
As shown in FIG. 6, the cell separation device 1 according to the present embodiment includes a cell stimulation container (container container, cell stimulation means) 22 and a turbidity sensor (state quantity measuring unit) arranged in the middle of the tube 15. Cell density meter) 23.

The inner surface of the cell stimulation container 22 is coated with a non-adhesive material to which fat-derived cells do not easily adhere. As the non-adhesive material, for example, silicone, phospholipid, polyhydroxyethyl methacrylate, ethylene vinyl alcohol copolymer and the like are used. Thereby, when a cell suspension is accommodated in the cell stimulation container 22, fat-derived cells that cannot adhere to the inner wall adhere to each other and aggregate.
The turbidity sensor 23 is arranged in the middle of the tube 15 where the cell suspension is conveyed from the collection container 8 to the cell stimulation container 22 and measures the turbidity of the cell suspension passing through the tube 15. In general, it is known that the cell density and turbidity in a cell suspension are in a positive proportional relationship.

  The computer 7 stores, for example, the relationship between the turbidity and the density of fat-derived cells by measuring the turbidity of a cell suspension whose density of fat-derived cells is known in advance. Thereby, the computer 7 calculates the density of the fat-derived cells contained in the cell suspension from the turbidity measured by the turbidity sensor 23. Further, the computer 7 presets the number of cells to be supplied into the cell stimulation container 22, calculates the supply amount of the cell suspension supplied to the cell stimulation container 22 based on the calculated density of the fat-derived cells, The liquid feeding time by the pump 15 is set.

The operation of the cell separation device 1 configured as described above will be described below.
When the cell separation device 1 according to the present embodiment generates a cell suspension in the collection container 8, the cell suspension is sent from the collection container 8 to the cell stimulation container 22. Then, when the number of cells in the cell stimulation container 22 reaches a predetermined value, the cell separation device 1 stops feeding the cell suspension and waits for a predetermined time so that fat-derived cells are stored in the cell stimulation container 22. Aggregate with each other. Subsequently, the cell separation device 1 conveys the cell suspension from the cell stimulation container 22 to the centrifuge container 12 to wash and concentrate the fat-derived cells.

  In this case, according to the present embodiment, the fat-derived cells are activated by contacting each other with an appropriate number of cells in the cell stimulation container 22. Thereby, there exists an advantage that the fat origin cell of a highly active state can be obtained by simple structure and operation, maintaining a closed system.

  In the above embodiment, the inner surface of the cell stimulation container 22 is coated with a non-adhesive material. Instead, a groove 22a is formed on the bottom surface of the cell stimulation container 22 as shown in FIG. May be. Even if it does in this way, if the fat origin cell A aggregates in the groove | channel 22a, it will sink and it will contact, and the fat origin cell A can be activated. At this time, it is preferable that the groove 22a is uniformly formed on the entire bottom surface so that the size of the aggregate of the fat-derived cells A does not vary.

Next, a third embodiment of the present invention will be described below with reference to FIG.
In this embodiment as well, as in the second embodiment, the same components as those in the first embodiment are denoted by the same reference numerals, description thereof is omitted, and differences from the first embodiment are mainly described. I will explain.

  As shown in FIG. 8, the cell separation device 1 according to the present embodiment is provided on the outside of the drug bag (cell stimulation means, activator supply unit) 24 that stores the activator solution and the collection container 8. Interface sensor (state quantity measuring unit) 18, heater 25 provided in holder 13a, and temperature sensor 26 for measuring the temperature of the collection container. The heater 25 and the temperature sensor 26 may have the same configuration as the heater 10 and the temperature sensor 11 described in the first embodiment.

  The drug bag 24 is connected to the centrifuge container 12 via the transport path 6. The drug bag 24 contains an activator solution prepared at a higher concentration than when used. As an activator, a growth factor and a NO generation factor are used.

  As the growth factor, bFGF, HGF, SDF-1, or the like is used. As the NO generating factor, endothlial NO synthase is used. Others: mitochondial potassuium channel openner, ACE inhibitor, tatin, angiotensin-converting enzyme inhibitor, angiotensin II tipe 1 receptor blocker, PPAR-gamma agonist, ertythropietin, Haeme Oxygenase, sphingosin-1-phosphate, FTY720, PPAR-gamma, agonist pioglitazone It is also possible to use.

  The computer 7 detects the interface of the cell suspension in the collection container 8 by the interface sensor 22 before the cell suspension is sent from the collection container 8 to the centrifuge container 12 after digesting the adipose tissue. To measure the volume of the cell suspension. Then, the computer 7 determines the final concentration of the activator solution when the activator solution is supplied to the cell suspension based on the measured amount of the cell suspension. The supply amount is calculated, and the calculated supply amount of the activator solution is supplied to the centrifuge container 12. Further, the computer 7 controls the temperature of the heater 23 so that the temperature measured by the temperature sensor 24 is maintained at the set value. It is preferable that the set value of the temperature of the temperature sensor 24 is appropriately selected depending on the activator used so that the activator acts on the fat-derived cells most efficiently.

The operation of the cell separation device 1 configured as described above will be described below.
When the cell separation apparatus 1 according to the present embodiment generates a cell suspension in the collection container 8, the cell suspension is transported to the centrifuge container 12 after measuring the amount of the cell suspension. . Subsequently, the cell separation device 1 supplies the activator solution in the centrifuge container 12 so that the concentration of the activator becomes a predetermined value, and waits for a predetermined time to thereby obtain the fat-derived cells and the activator. After sufficiently reacting, the fat-derived cells are washed to remove the activator together with the digestive enzyme solution.

  Thus, according to the present embodiment, it is possible to activate fat-derived cells with a simple configuration and operation, and improve the effect of the treatment performed thereafter, by adding an activator to the fat-derived cells. There is an advantage that you can. In addition, the fat-derived cells are efficiently activated by controlling the supply amount of the activator solution according to the liquid volume of the cell suspension and reacting the fat-derived cells with the activator at a predetermined temperature. There is an advantage that you can.

DESCRIPTION OF SYMBOLS 1 Cell separator 2 Decomposition | disassembly processing part 3 Cell concentration part 4 Washing liquid bag 5 Waste liquid bag 6 Conveyance path 7 Computer (control part)
8 Collection container (container)
8a Inlet 8b Bottom 8c Adipose tissue capture filter 8d Enzyme supply port 9 Shaker (stirring means)
10 Heater (cell stimulation means)
11 Temperature sensor (state quantity measuring unit)
12 Centrifuge container 12a Piping 13 Centrifuge (activator removal means)
13a holder 14 joint 15 tube 16 liquid feed pump 17 ultrasonic transducer (cell stimulation means)
18 Interface sensor (state quantity measuring unit)
19 Dissolved oxygen sensor (oxygen concentration sensor, state quantity measuring unit)
20 Gas cylinder (cell stimulation means, low oxygen gas supply part)
20a Piping 21 Air filter 22 Cell stimulation container (container)
23 Turbidity sensor (state quantity measuring unit, cell density meter)
24 drug bag (cell stimulation means, activator supply part)
25 Heater 26 Temperature sensor A Fat-derived cell V1 to V13 Valve

Claims (11)

A digestion processing unit that digests the living tissue to generate a cell suspension in which the cells are released from the living tissue;
A cell concentration unit for concentrating the cell suspension generated in the decomposition processing unit;
A storage container for storing the cell suspension;
A conveyance path connected to the decomposition processing unit, the cell concentration unit and the container, and conveying the cell suspension;
A state quantity measuring unit for measuring a state quantity representing the state of the cell suspension contained in the container;
Cell stimulation means for stimulating cells contained in a cell suspension accommodated in the container;
A cell separation apparatus comprising: a control unit that controls a magnitude of stimulation given to the cell by the cell stimulation unit based on the state quantity measured by the state quantity measurement unit. The state quantity measuring unit is a temperature sensor that measures the temperature of the cell suspension in the container,
The cell stimulation means includes a heater for heating the inside of the storage container,
The cell separation device according to claim 1, wherein the controller controls the temperature of the heater based on the temperature of the cell suspension measured by the temperature sensor.   The cell separation apparatus according to claim 2, further comprising a stirring unit that stirs the inside of the container. The state quantity measuring unit is a liquid quantity sensor for measuring the liquid quantity of the cell suspension in the container,
The cell stimulation means includes an ultrasonic transducer that generates ultrasonic waves in the container,
The cell separation device according to claim 1, wherein the control unit controls an output of an ultrasonic wave generated by the ultrasonic transducer based on a liquid amount of the cell suspension measured by the liquid amount sensor. The state quantity measuring unit is an oxygen concentration sensor that measures the oxygen concentration of the cell suspension stored in the storage container,
The cell stimulation means includes a low oxygen gas supply unit that supplies a gas having an oxygen concentration lower than air into the storage container,
The cell separation device according to claim 1, wherein the control unit controls a supply amount of the gas supplied from the low oxygen gas supply unit into the storage container based on an oxygen concentration measured by the oxygen concentration sensor. . The state quantity measuring unit is a liquid quantity sensor that measures the liquid quantity of the cell suspension stored in the storage container,
The cell stimulating means comprises an activator supply unit for supplying an activator for activating the cells in the container;
The cell according to claim 1, wherein the control unit controls the supply amount of the activator supplied from the activator supply unit into the storage container based on the liquid amount measured by the liquid amount sensor. Separation device.   The cell separation device according to claim 6, further comprising an activator removing means for removing the activator.   The cell separator according to claim 6, wherein the activator is a growth factor or a nitric oxide production factor. The state quantity measuring unit is a cell density meter that measures the density of the cells contained in the cell suspension in the container,
The cell stimulating means in the container to stimulate the cells by bringing the cells contained in the cell suspension into contact with each other;
The cell separation device according to claim 1, wherein the control unit controls the supply amount of the cell suspension supplied into the storage container through the transport path based on the density measured by the cell density meter.   The cell separation device according to claim 9, wherein the cell stimulating unit aggregates the cells by coating an inner surface of the storage container with a non-adhesive material to which the cells do not adhere.   The cell separation device according to claim 9, wherein the cell stimulating unit aggregates the cells in the groove by forming a groove on a bottom surface of the storage container.

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