JP2011182650A5 - - Google Patents

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JP2011182650A5
JP2011182650A5 JP2010048242A JP2010048242A JP2011182650A5 JP 2011182650 A5 JP2011182650 A5 JP 2011182650A5 JP 2010048242 A JP2010048242 A JP 2010048242A JP 2010048242 A JP2010048242 A JP 2010048242A JP 2011182650 A5 JP2011182650 A5 JP 2011182650A5
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harmful
toxic
oligonucleotide probe
base sequence
dna
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JP2010048242A
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JP5818195B2 (en
JP2011182650A (en
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有害又は有毒渦鞭毛藻類の染色体DNA中の大サブユニットリボソームRNA遺伝子の塩基配列にハイブリダイズし得る塩基配列を含む、有害又は有毒渦鞭毛藻類検出用オリゴヌクレオチドプローブ。 An oligonucleotide probe for detecting harmful or toxic dinoflagellates comprising a base sequence capable of hybridizing to the base sequence of a large subunit ribosomal RNA gene in chromosomal DNA of harmful or toxic dinoflagellates. 前記大サブユニットリボソームRNA遺伝子の塩基配列が、該リボソームRNA遺伝子のD1領域又はD2領域の塩基配列である、請求項１記載のオリゴヌクレオチドプローブ。 The oligonucleotide probe according to claim 1, wherein the base sequence of the large subunit ribosomal RNA gene is the base sequence of the D1 region or D2 region of the ribosomal RNA gene. 有害又は有毒渦鞭毛藻類が、Alexandrium属、Dinophysis属、Gambierdiscus属、Gymnodinium属、Karenia属、Ostreopsis属、Pfiesteria属、Prorocentrum属、Protoceratium属、Protoperidinium属及びPyrodinium属からなる群より選ばれる少なくとも１種である、請求項１又は２記載のオリゴヌクレオチドプローブ。 The harmful or toxic dinoflagellate is at least one selected from the group consisting of the genus Alexandrium, Dinophysis, Gambierdiscus, Gymnodinium, Karenia, Ostreopsis, Pfiesteria, Prorocentrum, Protoceratium, Protoperidinium and Pyrodinium The oligonucleotide probe according to claim 1 or 2 . 下記(a)又は(b)のDNAの塩基配列を含むものである、請求項１〜３のいずれか１項に記載のオリゴヌクレオチドプローブ。
(a) 配列番号4、7、8、10〜16、20、22、27、29、31〜35、39〜47及び50〜58に示される塩基配列からなるDNA
(b) 上記(a)のDNAに対し相補的な塩基配列からなるDNAとストリンジェントな条件下でハイブリダイズし、かつ有害又は有毒渦鞭毛藻類に由来する核酸の塩基配列のうちの少なくとも一部の塩基配列を検出し得る機能を有するDNA The oligonucleotide probe according to any one of claims 1 to 3 , which comprises the base sequence of the following DNA (a) or (b):
(a) DNA consisting of the nucleotide sequence shown in SEQ ID NOs: 4, 7, 8, 10-16, 20, 22, 27, 29, 31-35, 39-47 and 50-58
(b) at least a part of the base sequence of a nucleic acid that hybridizes with a DNA comprising a base sequence complementary to the DNA of (a) above under stringent conditions and is derived from a harmful or toxic dinoflagellate DNA having a function capable of detecting the nucleotide sequence of 請求項１〜４のいずれか１項に記載のオリゴヌクレオチドプローブが基盤に配置された、有害又は有毒渦鞭毛藻類検出用マイクロアレイ。 A microarray for detecting harmful or toxic dinoflagellates, wherein the oligonucleotide probe according to any one of claims 1 to 4 is arranged on a substrate. 複数の貫通孔を有し、それら貫通孔にゲルが保持されているマイクロアレイであって、前記ゲルに請求項１〜４のいずれか１項に記載のオリゴヌクレオチドプローブが保持されている、有害又は有毒渦鞭毛藻類検出用マイクロアレイ。 A microarray having a plurality of through-holes and a gel held in the through-holes, wherein the oligonucleotide probe according to any one of claims 1 to 4 is held in the gel. A microarray for detecting toxic dinoflagellates. 有害又は有毒渦鞭毛藻類の検出方法であって、
所定の海域から海水を採取して被検体とし、当該被検体中の生物に由来する核酸を抽出する工程、及び
前記抽出した核酸を請求項１〜４のいずれか１項に記載のオリゴヌクレオチドプローブ又は請求項５若しくは６記載のマイクロアレイに接触させる工程
を含む、前記方法。 A method for detecting harmful or toxic dinoflagellates,
The step of extracting seawater from a predetermined sea area as a specimen, extracting nucleic acids derived from living organisms in the specimen, and the oligonucleotide probe according to any one of claims 1 to 4 Alternatively, the method comprising the step of contacting the microarray according to claim 5 or 6 . 前記被検体が、所定の複数の海域から海水を採取して得られた複数の被験体である、請求項７記載の方法。 The method according to claim 7 , wherein the subject is a plurality of subjects obtained by collecting seawater from a plurality of predetermined sea areas. 請求項７又は８記載の方法により得られた検出結果を解析し、当該解析結果を指標として海洋状況を推測することを特徴とする、海洋モニタリング方法。 A marine monitoring method characterized by analyzing a detection result obtained by the method according to claim 7 or 8 and estimating a marine situation using the analysis result as an index. 請求項８記載の方法により得られた前記複数の被験体ごとの検出結果を比較解析し、当該解析結果を指標として海洋状況に対する地球温暖化の影響を推測することを特徴とする、海洋モニタリング方法。 9. A marine monitoring method, comprising comparing and analyzing detection results for each of the plurality of subjects obtained by the method according to claim 8, and estimating an influence of global warming on a marine situation using the analysis results as an index. . 前記複数の被験体ごとに、所定の有害又は有毒渦鞭毛藻類が検出されたか否かを比較することを含む、請求項１０記載の方法。 11. The method of claim 10 , comprising comparing for each of the plurality of subjects whether a predetermined harmful or toxic dinoflagellate has been detected. 所定の有害又は有毒渦鞭毛藻類が、Pyrodinium属及び／又はGambierdiscus属である、請求項１１記載の方法。 The method according to claim 11 , wherein the predetermined harmful or toxic dinoflagellate is of the genus Pyrodinium and / or Gambierdiscus.
JP2010048242A 2010-03-04 2010-03-04 Probes and microarrays for detecting harmful or toxic dinoflagellates Expired - Fee Related JP5818195B2 (en)

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JP2011182650A5 true JP2011182650A5 (en) 2013-03-14
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KR101706771B1 (en) * 2015-06-12 2017-02-17 한국해양과학기술원 Method for detection of Cochlodinium polykrikoides by in situ hybridization and kit therefor
KR101747684B1 (en) 2015-06-18 2017-06-15 한국해양과학기술원 Method for detection of Heterocapsa triquetra by sandwich hybridization integrated with nuclease protection assay and kit therefor

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JP2003344405A (en) * 2002-05-28 2003-12-03 Mitsubishi Rayon Co Ltd Probe immobilizing gel holding micro-array and specimen detection method using it
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