JP2011123518A5

JP2011123518A5 -

Info

Publication number: JP2011123518A5
Application number: JP2011021738A
Authority: JP
Filing date: 2011-02-03
Publication date: 2011-08-11
Anticipated expiration: 2024-07-08

Claims

A stage on which an observation sample is placed;
Photographing means for photographing the observation sample;
An objective lens arranged to face the observation sample placed on the stage;
A focusing drive unit for driving at least one of the stage and the objective lens;
An imaging condition setting unit for setting an imaging area upper limit and an imaging area lower limit of the observation sample;
An autofocus unit for controlling the focusing drive unit,
After performing autofocus on a predetermined position by the autofocus unit, the focusing drive unit performs offset driving to move at least one of the stage and the objective lens by a predetermined amount, and then The microscope system is characterized in that the imaging unit images at least two observation samples having different focal positions at predetermined intervals according to the setting of the imaging condition setting unit.

The microscope system according to claim 1, wherein the photographing condition setting unit sets at least one of the number of photographing, the pitch of the focusing drive unit, and the photographing time.

Wherein the predetermined position is a transparent body which is the reference position, the observation specimen microscope system according to claim 1 or claim 2, characterized in that it comprises an observation body and the transparent body.

The autofocus unit is
A focus detection light source that emits autofocus detection light to the observation sample;
The microscope system according to any one of claims 1 to 3, further comprising a light receiving unit that receives the detection light reflected by the observation sample again through an objective lens.

A stage for placing an observation sample comprising a transparent body and an observation body placed on the transparent body;
An objective lens disposed below the stage so as to face the observation sample placed on the stage;
A focusing drive unit for driving at least one of the stage and the objective lens in a direction parallel to the observation optical axis;
An observation light source for illuminating the sample;
An imaging device that photographs the observation sample via the objective lens;
A focus detection light source that emits detection light for autofocus from the lower side of the objective lens to the observation sample with a light source different from the observation light source
A light receiving means for receiving the detection light reflected from the transparent body in the observation sample through an objective lens;
An autofocus unit capable of autofocusing to a predetermined position of the transparent member by controlling the focusing drive unit from the result of the light receiving means;
An imaging condition setting unit configured to set the imaging positions of the plurality of observation objects having different focal positions apart from the predetermined position by a predetermined amount on the basis of the predetermined position;
Performing autofocus on the predetermined position, which is a position where the focus detection light is reflected from the transparent member by the autofocus unit;
After completion of autofocusing to the predetermined position, at least two images of the observation specimen having different focal positions at a plurality of positions apart from the predetermined position set by the imaging condition setting means are displayed. To shoot
A microscope system characterized by

An XY stage driving unit that drives and controls the stage perpendicular to the observation optical axis is further provided, and the predetermined amount is set for each stage XY address that is previously positioned by the XY stage driving unit. The microscope system according to any one of claims 1 to 5, wherein:

The shooting condition setting unit sets a plurality of shooting positions by setting at least one of a pitch of the focusing drive unit based on the predetermined position, an upper and lower limit position indicating a shooting range, and a number of shots. The microscope system according to claim 6, wherein the microscope system is set.

In an observation method comprising an image sensor and photographing an observation sample according to photographing conditions,
Performing autofocus on a predetermined position;
Driving to the upper limit of the imaging area of the observation sample;
An imaging method comprising: imaging the observation sample having at least two focal positions differing at predetermined intervals in accordance with the imaging conditions while driving from the imaging area upper limit to the imaging area lower limit. .

In an observation method comprising an image sensor and photographing an observation sample according to photographing conditions,
Performing autofocus on a predetermined position;
A step of driving the imaging area and low limits of the observation specimen,
During the driving the imaging area upper limit from the imaging area and low limits, characterized in that at least two or more focal position and a step of performing photographing of said different observation sample at predetermined intervals according to the imaging condition Observation method.