JP2011092133A - Method for simply identifying moldy grain - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for simply identifying moldy grains capable of precisely inspecting mold without relying the experience of a worker, simple in mold inspection work and requiring no special microbial inspection facilities or machinery/instruments and materials. <P>SOLUTION: The method for simply identifying the moldy grain includes the process for preparing a mold cultivation container equipped with a resin body which houses a mold detection medium and a resin lid for sealing the opening of the resin body, the process for opening the opening of the resin body sealed by the resin lid, the process for placing grains on the mold detection medium from the opening, and the process for keeping the mold cultivation container at room temperature after covering the opening with the resin lid. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a simple identification method of mold-caused grains, and more specifically, mold-like foreign substances in mold-like foreign substances inspection for grain quality control without having a special culture facility or inspection experience. The present invention relates to a method for quickly and easily discriminating cereal grains.

  In the storage of cereals, the maintenance of quality and ensuring safety are based on temperature / humidity control during storage, control of the moisture content of cereals, and prevention of pest damage. In particular, cereal grains may suffer from problems such as storable molds growing and being damaged, or mold toxins accumulating due to mold toxin-producing bacteria. Ingestion of grains infested with mold poisons has a great effect on the human body, so molds stored in storage or the like need mold inspection.

  Conventionally, an operator samples a sample suspected of generating mold in a circular tray for inspection (carton), and visually observes the sample on the sampled carton to confirm the presence or absence of mold. If it is difficult to determine the presence or absence of mold by visual observation, the sample is placed on a mold / agar culture medium for a yeast culture contained in a petri dish at a microorganism testing facility or the like, and the mold is cultured. A method of observing and measuring colonies that appeared after 7 days and confirming the presence or absence of mold has been adopted.

  However, the above visual confirmation method has a problem that the accuracy of mold inspection must depend on the experience of the operator. In addition, in a facility that can be aseptically operated to cultivate mold, an agar medium must be prepared immediately before the mold culturing operation and the petri dish body must be filled with the agar medium, which is cumbersome. In addition, when placing a test sample on the agar medium, the lid body separate from the petri dish body is removed from the petri dish, and the removed lid body is put on the petri dish body again due to poor handling. If the person's experience is inexperienced, there is a problem that contamination of the medium is likely to occur and a lot of personnel are required for the mold inspection work.

  Thus, for example, when the sample suspected of generating mold is brown rice, a method has been proposed in which the aroma component in the brown rice where mold has occurred is detected to identify the brown rice where mold has occurred (Patent Document 1). However, in this identification method, it is necessary to collect and condense aroma components and then detect them using a gas chromatograph mass spectrometer or a conductive polymer type sensor, which requires expensive equipment and equipment. There is also a problem that the inspection object of mold is limited to brown rice.

JP-A-11-94821

  In view of the above circumstances, the present invention is capable of accurate mold inspection without relying on the operator's experience, is simple in mold inspection work, and does not require a special microorganism testing facility, equipment or equipment. An object is to provide a method for identifying generated grains.

  The first aspect of the present invention is a step of preparing a mold culturing container comprising a resin main body in which a mold detection medium is accommodated and a resin lid body in which an opening of the resin main body is sealed. And opening the opening of the resin main body sealed by the resin lid, placing the grain on the mold detection medium from the opened opening, and opening the opening And a step of covering the opened opening with the resin lid and storing the mold culturing container at room temperature.

  The mold detection medium is sterilized and stored in the resin main body of the mold culture container. The operator opens the resin lid of the mold culturing container and puts multiple test grains arbitrarily selected from the lots suspected of mold generation into the mold detection medium contained in the resin body one by one. Leave them at predetermined intervals. Then, after the resin lid of the mold culturing container is closed again, the mold culturing container is stored at room temperature. Several days later, each test grain is visually checked for mold growth. Then, among the test grains placed on the mold detection medium, a lot that contains a predetermined ratio of the test grains in which mold growth is recognized is determined as a mold-producing sample, and predetermined treatment such as disposal is performed. The term “cereal” means a crop that is cultivated to make the seeds edible among agricultural crops, and examples thereof include small seeds such as rice and wheat grains.

  According to a second aspect of the present invention, instead of the resin lid body, a storage case body made of resin that houses the resin body inside and an opening of the storage case body sealed for storage. A simple identification method for moldy grain, comprising a case lid.

  In this embodiment, the resin main body containing the mold detection medium is aseptically stored in the resin storage case, and the operator opens the storage case lid to store the resin main body. Take it out of the case body or store it in the storage case body for mold inspection.

  According to a third aspect of the present invention, there is provided a simple identification method for moldy grain, wherein the resin lid is a film or a slide-movable plate. According to a fourth aspect of the present invention, there is provided a simple identification method for moldy grain, wherein the storage case cover is a film or a slide-like plate.

  In this aspect, when the resin lid is a film, the periphery of the film is fixed to the top of the periphery of the resin main body forming the opening of the resin main body, thereby sealing the opening of the resin main body. When the film covering the opening is peeled off from the top of the periphery of the resin main body, the opening is opened. For example, the opened opening can be re-adhered with the adhesive tape using the adhesive tape between the film and the peripheral top of the resin main body, or can be fitted to the overlapping portion of the peripheral edge of the film and the peripheral top of the resin main body. By using the fixing member, it can be closed again.

  When the resin lid is a slidable plate-like body, the plate-like body is slidably guided by a concave groove provided in the inner wall of the resin body to cover the opening of the resin body. Stop. When the plate-like body covering the opening is slid in a direction parallel to the bottom surface of the resin main body, the opening is opened. The opened opening can be closed again by sliding the plate-like body to the original position.

  According to a fifth aspect of the present invention, the mold detection grain is characterized in that the mold detection medium is an agar medium, and the agar concentration of the agar medium is 1.15 to 1.35%. This is a simple identification method. While the agar concentration of a normal agar medium is 1.5 to 1.6%, in the above embodiment, the agar concentration is 1.15 to 1.35%, which is slightly soft.

  According to a sixth aspect of the present invention, on the inner bottom surface of the resin main body in which the mold detection medium is accommodated, a partition portion that divides the inside of the resin main body into a plurality of compartments in plan view is provided. A simple identification method for moldy grain, wherein one grain is placed in each of the divided sections. That is, in this aspect, the inner bottom surface of the resin main body is divided into a plurality of compartments by the partition, and the fungus detection medium is accommodated in each compartment.

  According to a seventh aspect of the present invention, there is provided a simple identification method for mold-producing grains, wherein a dimension of the partition portion in a height direction is larger than a thickness dimension of the mold detection medium. That is, in this aspect, the mold detection medium contained in each compartment is not in contact with the mold detection medium contained in the adjacent compartment, and the mold detection medium contained in each compartment is the other compartment. The mold detection medium contained in each is independent of each other.

  According to an eighth aspect of the present invention, there is provided a simple identification method for moldy grain, wherein the resin main body is rectangular in a plan view.

  According to the first aspect of the present invention, the test grain is set in the mold culture container by opening and closing the opening with the lid integrally provided with the main body, so that the test grain can be set quickly and easily. It is. Thus, since the handling property of the set operation | work of a test grain is excellent, the contamination of the culture medium during a set operation can be prevented. This eliminates the need for special equipment and equipment to prevent medium contamination, such as a clean bench, and can be used to identify mold with grain in a relatively clean room inside or outside the storage warehouse. .

  According to the second aspect of the present invention, since the resin main body in which the mold detection medium is stored is stored in the resin storage case, the resin main body when storing the mold culture container is used. Damage can be prevented.

  According to the third and fourth aspects of the present invention, since the lid body is a film or a slidable plate-like body, the opening / closing operation of the opening can be performed easily and quickly.

  According to the fifth aspect of the present invention, by setting the agar concentration of the agar medium to 1.15 to 1.35%, the hardness of the test grain can be embedded in the medium. The position of the inspection grain can be fixed.

  According to the sixth aspect of the present invention, the mold detection medium is divided into a plurality of sections, and one test grain is placed in each section. Therefore, the positioning of the test grains on the medium is easy, and the mold The inspection work can be speeded up. In addition, since each test grain is placed separately for each compartment, it is easy to identify the sample even if different types of samples are arranged in the same mold culture container.

  According to the seventh aspect of the present invention, since the dimension in the height direction of the partition portion is larger than the thickness dimension of the mold detection medium, even if the storage state of the mold culture container cannot be kept horizontal, It is possible to prevent a sample placed in a specific section from moving to another section. Moreover, since the partition part protrudes from the surface of the mold detection medium, it is possible to prevent mold generated in a predetermined sample from advancing to another adjacent section. Therefore, it is possible to more accurately grasp the proportion of the test grains in which mold has occurred, and it is also possible to handle a plurality of types or lots of sample inspection with a single mold culture container.

  According to the eighth aspect of the present invention, since the resin main body is rectangular in plan view, compared to a resin main body having a curved portion such as a petri dish, the area of the entire main body is substantially the same even though it is substantially the same area. In other words, it is possible to increase the set quantity of inspection grains. In this way, since the number of inspection objects can be increased with one mold culture container, the accuracy of the inspection is improved. In addition, since the area of each section can be made uniform, it is possible to suppress the growth of the grown mold to the adjacent sample, and to reliably identify the sample in which mold is observed.

(A) The figure is a top view of the mold cultivation container used for the simple identification method according to the first embodiment of the present invention, and (b) the figure is the mold used for the simple identification method according to the first embodiment of the present invention. It is sectional drawing of the container for culture | cultivation. It is explanatory drawing of the opening process of the opening part of the simple identification method which concerns on the example of 1st Embodiment of this invention. It is explanatory drawing of the sample mounting process of the simple identification method which concerns on the example of 1st Embodiment of this invention. It is explanatory drawing of the process of closing the opening part of the simple identification method which concerns on the example of 1st Embodiment of this invention. It is explanatory drawing of the storage process of the simple identification method which concerns on the example of 1st Embodiment of this invention. It is a figure which shows the state which the mold | fungi generate | occur | produced in the Example of this invention. (A) The figure is a top view of the mold cultivation container used for the simple identification method according to the second embodiment of the present invention, and (b) the figure is the mold used for the simple identification method according to the second embodiment of the present invention. It is sectional drawing of the container for culture | cultivation.

  Next, a simple identification method for moldy grain according to an embodiment of the present invention will be described with reference to the drawings.

  The first embodiment of the present invention provides a mold culturing container comprising a resin main body containing a mold detection medium and a resin lid body in which an opening of the resin main body is sealed. A step of opening an opening of the resin main body sealed by the resin lid, a step of placing rice grains on the mold detection medium from the opened opening, Covering the opened opening with the resin lid and closing the opening again; and storing the mold culturing container at room temperature to culture mold.

  As shown in FIGS. 1A and 1B, the mold culturing container 1 used in the first embodiment of the present invention has a resin main body 2 and a shape corresponding to the shape of the main body 2 in plan view. It is comprised from the film-form resin-made cover body 3 which has. The main body 2 is filled with a mold detection medium 4 which is an agar medium. A flat portion 6 that is substantially parallel to the bottom surface of the main body 2 is provided around the periphery on the opening 5 side of the main body 2 so as to face the outside of the main body 2. The plane portion 6 provided around the opening 5 has substantially the same width. In addition, a rectangular flat plate portion 9 substantially parallel to the bottom surface of the main body 2 is disposed on one of the two flat surface portions 6 perpendicular to the longitudinal direction of the main body 2 toward the outside of the main body 2. Projected. Further, two protrusions protruding toward the lid 3 are formed on the surface of the flat plate portion 9.

  The opening 5 of the main body 2 is sealed by fixing the film-like lid 3 to the flat portion 6. On the other hand, the film-like lid 3 is not fixed to the flat plate portion 9. Accordingly, the operator grasps the portion of the lid 3 that is not fixed to the flat plate portion 9 by using the projections, and then pulls the portion in the longitudinal direction of the main body 2, thereby removing the lid 3 from the main body 2. Can be easily peeled off.

  Further, an entry site 21 is provided on the surface of the lid 3 at a position corresponding to the flat plate portion 9. The entry part 21 is used, for example, for describing the lot number of the mold culture container 1 or the sample name of the test grain 20. Here, the entry portion 21 is obtained by applying a white paint to the surface of the lid 3. In addition, the dimension of the main body 2 which concerns on the said 1st Embodiment is 125 mm x 55 mm including the plane part 6 and the flat plate part 9, and the depth is 10 mm.

  The inside of the main body 2 sealed with the lid 3 and the mold detecting medium 4 accommodated in the main body 2 are both sterilized. Therefore, the mold culture container 1 has a structure in which the inside of the main body 2 and the mold detection medium 4 are not contaminated unless the lid 3 is peeled off from the flat portion 6. In addition, on the inner bottom surface of the main body 2, four partition portions 7 are parallel to the longitudinal direction of the main body 2, and four partition portions 8 are perpendicular to the longitudinal direction of the main body 2. The main body 2 is divided into 25 sections. The dimension in the height direction of the partition parts 7 and 8 is not more than the depth dimension of the main body 2 and is larger than the thickness dimension of the mold detecting medium 4.

  The mold detection medium 4 is not particularly limited as long as it is a medium that can be used for separation of mold and yeast from cereals and count of bacteria. A commonly used one is, for example, YM agar medium. In addition, the preferable mold detection medium 4 is suitable as a selective medium for calculating and separating filamentous fungi and yeasts involved in food deterioration, and can suppress the growth of expandable mold. DRBC (Dichloran-Rose-bengal- Chloramphenicol) DG18 (Dichloran-Glycerol) agar medium, mold toxin in that it is suitable for isolating and calculating psychrophilic filamentous fungi from dried or semi-dried foods and can suppress the growth of expandable mold AFPA (Aspergillus Flabus / Par) is suitable for rapid isolation and count of Aspergillus flavus and Aspergillus parasiticus that produce aflatoxin. asiticus Agar) medium.

  The worker samples a rice sample from a lot of rice stored in the storage warehouse, which is suspected of generating mold, with a carton, and visually observes whether or not mold is generated. However, when it is difficult to determine whether mold has occurred, the worker brings the sampled rice grains into a relatively clean room near the warehouse. Then, as shown in FIG. 2, the mold culture container 1 prepared in advance is picked up, and the lid 3 is peeled off from the flat part 6 and sealed in the opening 5. To open. At this time, the lid 3 may be completely separated from the main body 2, but the lid 3 is not completely separated from the main body 2 in terms of facilitating workability in the process of closing the opening 5 described later. It is preferable that a part of the lid 3 is fixed to the flat portion 6 of the main body 2 to the extent that there is no problem in arranging rice grains on the mold detection medium 4.

  Then, as shown in FIG. 3, the worker collects rice grains sampled with a carton with tweezers. That is, the rice grain collected with the tweezers becomes the inspection grain 20. Thereafter, the operator places the test grains 20 side by side in each compartment so as to lightly press the test grains 20 against the surface of the mold detection medium 4. The agar concentration of the mold detection medium 4 is 1.15 to 1 in that the lower part of each test grain 20 is buried in the mold detection medium 4 to prevent each test grain 20 from rolling in the mold culture container 1. .35%, and preferably 1.20 to 1.30% from the viewpoint of reliably preventing the mold detection medium 4 from cracking and facilitating the mounting work on the mold detection medium 4.

  As shown in FIG. 4, after placing the test grains 20 in all 25 sections, the operator opens the lid 3 that is not completely separated from the main body 2 and is partially fixed to the main body 2. 5 is returned to the position before opening, and the opening 5 is closed again. The fixing means for the peripheral portion of the lid 3 and the flat portion 6 of the main body 2 is not particularly limited. For example, it can be bonded by an adhesive tape, or the overlapping portion of the peripheral portion of the lid 3 and the flat portion 6 of the main body 2 can be used. For example, fixing by fitting a fitting member that can be fitted is used. In the embodiment shown in FIG. 4, the fixing member 10 is used.

  When the lid 3 is returned to the original position and the opening 5 is closed, as shown in FIG. 5, the operator stores the mold culture container 1 in the relatively clean room at room temperature for 48 to 72 hours, Observe and record the occurrence of mold. If necessary, take a recorded photo with a camera or the like.

  After the cultivation for 48 to 72 hours, out of 25 test grains 20, for example, when mold growth is observed in 1/5 or more and less than half, it is determined that there is a suspicion of mold generation sample, and if necessary Repeat the test. For example, when mold growth is recognized in more than half of the 25 test grains 20, the mold is determined to be a mold-producing sample, and the corresponding lot is subjected to predetermined treatment such as disposal. Moreover, when the growth of mold | fungi was recognized among 25 test | inspection grains 20, for example, less than 1/5, it determines with a mold | fungi non-occurrence | production sample. In addition, the mold culturing container 1 after use is not sterilized under high temperature and high pressure, but can be discarded as it is after being immersed in, for example, a commercially available chlorine bleach.

  Next, a second embodiment of the present invention will be described. In addition, the same code | symbol is attached | subjected about the same component as the said 1st Embodiment. In the first embodiment, the mold culturing container 1 composed of the resin main body 2 filled with the mold detection medium 4 and the film-like resin lid body 3 is used. In the second embodiment, as shown in FIG. 7, a resin storage case body 12 in which a resin main body 2 filled with a mold detection medium 4 is housed, and a plan view of the storage case main body 12. The mold culturing container 11 provided with a film-like resin storage case lid 13 having a shape corresponding to the above shape is used.

  In the second embodiment, a flat portion 16 that is substantially parallel to the bottom surface of the storage case main body 12 is provided around the periphery of the storage case main body 12 toward the outside of the storage case main body 12. Yes. The plane portion 16 provided around the opening 15 has substantially the same width. A rectangular flat plate portion 19 that is substantially parallel to the bottom surface of the storage case main body 12 is stored in one of the two flat surface portions 16 perpendicular to the longitudinal direction of the storage case main body 12. It protrudes toward the outside of the case main body 12. The film-shaped storage case lid body 13 is fixed to the flat surface portion 16 so that the opening 15 of the storage case body 12 is sealed. Therefore, the film-like lid 3 is not fixed to the flat portion 6 of the main body 2.

  As shown in FIG. 7, the housing case lid 13 is provided with two main body pressing portions 22. The main body pressing portion 22 is a rectangular protrusion provided on the main body 2 side of the housing case lid 13, and is provided at a position facing the corner of the side wall of the main body 2 far from the flat plate portion 19. The main body pressing portion 22 presses the corner of the side wall of the main body 2 accommodated in the housing case main body 12 from above, so that the main body 2 is supported in the housing case main body 12. Thereby, the main body 2 is prevented from moving in the housing case main body 12.

  The film-like storage case lid 13 is not fixed to the flat plate portion 19. Accordingly, the operator can easily grasp the portion of the storage case lid 13 that is not fixed to the flat plate portion 19 and then pull the portion in the longitudinal direction of the storage case main body 12 to easily remove the portion from the storage case main body 12. The storage case lid 13 can be peeled off.

  Further, the storage case lid 13 is provided with an entry portion 21 at a position corresponding to the flat plate portion 19. The entry part 21 is used, for example, for describing the lot number of the mold culture container 11 or the sample name of the test grain 20. Here, the entry portion 21 is obtained by applying a white paint to the surface of the housing case lid 13.

  Both the inside of the storage case main body 12 sealed by the storage case lid 13 and the mold detection medium 4 stored in the main body 2 are in a sterilized state. Therefore, the mold culture container 11 has a structure in which the inside of the storage case main body 12 and the mold detection medium 4 are not contaminated unless the storage case lid 13 is peeled off from the flat portion 16. Similarly to the first embodiment, the inner bottom surface of the main body 2 has four partition portions 7 in a direction parallel to the longitudinal direction of the main body 2 and is perpendicular to the longitudinal direction of the main body 2. Four partition sections 8 are provided at regular intervals, and the inside of the main body 2 is divided into 25 sections. The dimension of the partition portions 7 and 8 in the height direction is equal to or less than the depth dimension of the storage case main body 12 and larger than the thickness dimension of the mold detecting medium 4.

  Hereinafter, although not shown, each step according to the second embodiment using the mold culturing vessel 11 will be described. First, the housing case lid 13 is peeled off from the flat portion 16 to open the sealed opening 15. At this time, the storage case lid 13 is not completely separated from the storage case main body 12 in order to facilitate workability in the process of closing the opening 15 described later, and the main body that stores the mold detection medium 4 It is preferable that a part thereof is fixed to the flat portion 16 of the housing case main body 12 to the extent that there is no hindrance to taking 2 out of the mold culturing vessel 11.

  Then, the operator puts a finger into the gap formed between the flat plate portion 9 of the main body 2 accommodated in the storage case main body 12 and the storage case main body 12 and grasps the flat plate portion 9 of the main body 2. The main body 2 is taken out from the mold culture container 11. Then, the test grains are arranged on the surface of the mold detection medium 4 taken out.

  When the test grains are arranged on the surface of the mold detection medium 4, the main body 2 is returned to the original position in the storage case main body 12, and a part of the main body 2 is fixed to the storage case main body 12. Is returned to its original position, and the opening 15 is closed again. The fixing means for the peripheral portion of the storage case lid 13 and the flat portion 16 of the storage case main body 12 is not particularly limited. For example, as in the first embodiment, for example, adhesion with an adhesive tape, storage case lid Examples include fixing by attaching a fixing member that can be fitted to the overlapping portion of the peripheral portion of the body 13 and the flat portion 16 of the housing case main body 12. The subsequent storage process of the mold culturing container 11 and the method for determining whether mold has occurred are the same as in the first embodiment.

  Next, another embodiment of the present invention will be described. In the first embodiment and the second embodiment, film-like ones are used for the lid 3 and the storage case lid 13 of the mold culturing containers 1 and 11, and the lid 3 and the storage case lid. 13 is fixed to the flat portions 6 and 16 of the main body 2 and the storage case main body 12. Instead of this, the cover body and the storage case cover body are slidably movable plate-like bodies, and the cover body and the storage body. It is good also as a form which opens and closes the opening parts 5 and 15 by slidably moving the case cover body.

  In the first embodiment example and the second embodiment example, the inspection object is rice, but it is not particularly limited to rice as long as it is a grain, and for example, it can also be used for wheat and beans.

  In the first embodiment example and the second embodiment example, the same type of test object is placed on all of the 25 compartments of the main body 2. However, for example, different types of test objects such as rice and beans may be placed. The number of partitions provided in the main body 2 is not limited to 25, and the number of partitions can be changed as appropriate by changing the number of partition portions 7 and 8. Further, in the first embodiment and the second embodiment described above, a part of each test grain 20 is buried in the mold detection medium 4, but the test grain 20 is not buried in the mold detection medium 4 on the surface thereof. In this case, the agar concentration of the mold detecting medium 4 may be increased to make the mold detecting medium 4 harder than the first embodiment and the second embodiment.

  Moreover, in the said 1st Embodiment and 2nd Embodiment, although the entry site | part 21 applied white paint to the surface of the cover body 3 and the storage case cover body 13, it replaced with this, The surface may be processed to be rough and have a cloudy appearance, and may be omitted if not necessary. Furthermore, although the flat plate portions 9 and 19 are provided in the first embodiment and the second embodiment, they may be omitted as appropriate. Further, in the second embodiment, the main body 2 is taken out from the mold culturing container 11 and the test grains are arranged. Alternatively, the test grain may be arranged while the main body 2 is stored in the mold culturing container 11. Good.

  Next, the present invention will be further described with reference to examples, but it is needless to say that the present invention is not limited to these disclosures.

Example 1
-Mold culture container used : Mold culture container shown in Fig. 1 In other words, a container composed of a main body in which a mold detection medium was accommodated and a film-like lid that sealed the opening of the main body was used.

-Mold detection medium used : DRBC agar composition Glucose ... 10.0 g / L
Peptone ... 5.0g / L
Potassium dihydrogen phosphate ... 1.0 g / L
Magnesium sulfate ... 0.5g / L
Dichlorane: 0.002 g / L
Rose Bengal: 0.025g / L
Chloramphenicol · 0.100g / L
Agar ... 13.0g / L
Purified water ... 1.0L
pH 5.6 ± 0.2
Preparation method After heating and dissolving, a DRBC agar medium was prepared by autoclaving at 121 ° C for 15 minutes.

  After sterilization, the mixture was naturally cooled to 50 ° C., and then the DRBC agar medium was thoroughly stirred and dispensed into 25 compartments of sterilized mold culture containers. At this time, the dispensing amount of each compartment was such that the thickness dimension of the DRBC agar medium was smaller than the dimension in the height direction of the partition part forming 25 compartments. After dispensing, the opening was sealed with a film-like lid by a predetermined method, and the mold culturing vessel was prepared. This mold culturing container was prepared in advance and stored in a predetermined storage place so that it could be used immediately if necessary.

  Among the stored polished rice stored in the storage, a part of the lot was sampled using a carton for the lot that was suspected of generating mold. The polished rice grains on the carton were visually observed to check for the presence of mold. Then, in order to determine the presence or absence of mold using a mold culturing container for a sample for which it is difficult to determine the presence or absence of mold by visual observation, the polished rice grains on the carton were transferred from the storage to a relatively clean room. Then, the lid of the mold culturing container stored therein was peeled off from the main body, and the opening of the sealed mold culturing container was opened. After opening, 25 grains were arbitrarily selected from the milled rice grains on the carton, and arranged on the mold detection medium divided into 25 sections of the mold cultivation container. Then, the opening part was closed again with the said cover body by attaching the member which can be fitted to the overlap part of the peripheral part of a cover body and the plane part of a main body. Then, the mold culture container was stored at room temperature for 72 hours, and the mold was cultured. The result is shown in FIG.

(Example 2)
Milled rice grains were cultured under the same test conditions and lots as in Example 1 except that the following YM medium was used as the mold detection medium.

-Mold detection medium used : YM medium composition Glucose ... 15.0 g / L
Soyton ... 6.0g / L
Malt extract ... 5.0g / L
Yeast extract ... 1.0g / L
Agar ... 16.0g / L
Rose Bengal, Streptomycin, Chloramphenicol Add as appropriate Purified water ... 1L
Preparation method After hot-dissolving, YM medium was prepared by autoclaving at 121 ° C for 15 minutes.

  Although not shown, in Example 2, generation of mold was observed in 8 out of a total of 25 polished rice grains arranged in each of the 25 compartments in the mold culture vessel.

  (Comparative Example) The same DRBC agar medium as used in Example 1 was prepared except that the amount of agar added to the DRBC agar basic medium was 15.0 g / L, which was the conventional composition. The DRBC agar medium was autoclaved at 121 ° C. for 15 minutes. After sterilization, the DRBC agar medium was naturally cooled to 50 ° C., stirred well, and dispensed into five sterilized petri dishes. In the comparative example, five petri dishes filled with this DRBC agar medium were used as mold cultivation containers. In a relatively clean room, remove the lid of the petri dish for each of the five petri dishes, select 5 rice grains arbitrarily from the rice grains on the same carton as in the example, and place them on the medium so that they are visually evenly spaced. The rice grains were arranged in a row. Thereafter, the petri dish body was covered with a lid, and stored at room temperature for 72 hours in the same manner as in the example.

  In addition, in the comparative example, the reason why the number of polished rice grains arranged in one petri dish was 5 is as follows. 2) Since the inner bottom surface of the petri dish is not fractionated by a plurality of partitions, the distance between the above polished rice grains is sufficient to prevent the grown mold from reaching other adjacent rice grains. Depending on what needs to be taken. If mold occurs on a given rice grain in a state where 6 or more grains are arranged in one petri dish, it becomes easier for grown mold to reach other adjacent rice grains, and the percentage of the rice grains with mold cannot be determined. .

  In Example 1, as shown in FIG. 6, generation | occurrence | production of mold | fungi was able to be observed in 9 grains of 25 grains of polished rice and 8 grains of 25 grains of polished rice in Example 2, respectively. That is, in Examples 1 and 2, the growth of mold was observed in 1/5 or more and less than half, so in both cases of Examples 1 and 2, the lot sampled in the carton was suspected of generating mold. I was able to judge it. Further, in both Examples 1 and 2, there was no contamination of the medium that would make it difficult to determine the presence or absence of mold on the mold detection medium, and an accurate determination was possible. This is thought to be because the opening / closing operation of the opening by the lid was performed quickly.

  On the other hand, although not shown, in the comparative example, four petri dishes with mold on the polished rice grains and one petri dish without mold were observed in the same lot as the example. Therefore, in the comparative example, unless the number of petri dishes is increased, it is not possible to ensure the accuracy of inspection for the occurrence of mold, and in one petri dish, compared to the mold culture container according to one embodiment. Inspection accuracy was found to be inferior. Moreover, in the comparative example, contamination of the medium was observed, and it was somewhat difficult to determine whether or not mold was generated on the polished rice grains as the test grains. Furthermore, in the comparative example, it is difficult to measure the number of milled rice grains due to medium contamination, and lots that should be determined to be mold suspicious, or lots that should be determined as mold generation samples. I could not judge either. This is probably because the opening / closing operation of the opening by the lid and the operation of arranging the polished rice grains could not be performed quickly.

  Since the present invention can easily and accurately confirm the presence or absence of mold generation without relying on the operator's experience and without using special equipment, it is useful in the field of identifying stored grains with mold generation. high.

DESCRIPTION OF SYMBOLS 1 Mold culture container 2 Main body 3 Lid 4 Mold detection culture medium 5 Opening part 7 Partition part 8 Partition part 11 Mold culture container 12 Storage case main body 13 Storage case cover 15 Opening part

Claims (8)

Preparing a mold culturing container comprising: a resin main body containing a mold detection medium; and a resin lid body sealing the opening of the resin main body;
Opening the opening of the resin main body sealed by the resin lid;
From the opened opening, placing the grain on the mold detection medium,
Covering the opened opening with the resin lid, and then storing the mold culturing container at room temperature.   Instead of the resin lid, a resin storage case main body that houses the resin main body, and a storage case lid that seals the opening of the storage case main body. 2. The simple identification method for moldy grain according to claim 1 characterized by the above-mentioned.   2. The method for easily identifying a moldy grain according to claim 1, wherein the resin lid is a film or a slide-like plate.   3. The method for easily identifying a moldy grain according to claim 2, wherein the storage case lid is a film or a slidable plate-like body.   The simple identification of mold-produced grains according to claim 1, wherein the mold detection medium is an agar medium, and the agar concentration of the agar medium is 1.15 to 1.35%. Method.   On the inner bottom surface of the resin main body in which the mold detection medium is accommodated, a partition portion that divides the inside of the resin main body into a plurality of compartments in a plan view is provided, one grain in each of the divided compartments The method according to claim 1, further comprising the step of:   The method for easily identifying mold-generated grain according to claim 6, wherein a dimension of the partition portion in a height direction is larger than a thickness dimension of the mold detection medium.   The method for easily identifying moldy grain according to claim 1, wherein the resin main body is rectangular in plan view.