CN102051403B - Simple recognition method of mildew cereal - Google Patents
Simple recognition method of mildew cereal Download PDFInfo
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- CN102051403B CN102051403B CN201010533845.9A CN201010533845A CN102051403B CN 102051403 B CN102051403 B CN 102051403B CN 201010533845 A CN201010533845 A CN 201010533845A CN 102051403 B CN102051403 B CN 102051403B
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Abstract
The invention is to provide a recognition method of mildew cereals, wherein the mold inspection of high accuracy can be easily performed without the experience of workers or special microbial inspection instruments.The simple recognition method of mildew cereals is characterized by comprising the following process:a process of preparing a container for culturing mould, wherein the container comprises a resin body for placing the culture medium for inspecting the mold and a resin cover for sealing the opening part of the resin body;a process of opening the opening part of the resin body sealed by the resin cover;a process of carrying cereals on the culture medium for inspecting the mold from the opening part;and a process of storing the container for culturing the mold after the opening part of the resin body is covered by the resin cover.
Description
Technical field
The present invention relates to the Simple recognition method of musty grain, more specifically, in the mustiness inspection of foreign substance of the qualitative control for cereal, in the situation that not having special cultivation facility or the experience of inspection, simple and easy promptly identification has produced the method for the cereal of mustiness foreign matter.
Background technology
In the keeping of cereal, in order to keep its quality and to guarantee security, be substantially temperature humidity, the moisture content of control cereal etc. and the generation of preventing disease and pest in advance in management keeping.Particularly cereal is in its keeping, sometimes occurring give birth to Storage mould fungi and is injured or accumulates the problems such as mould poison because of mould poison generation bacterium.If picked-up is by the cereal of mould poison infringement, bring greatest impact to human body, so keeping must be carried out mould inspection in the cereal in storage etc.
At present, operating personnel suspects mouldy sample, the visual inspection sample on the carton obtaining of sampling checking with gathering in circular basin (carton, carton), and confirmation has or not mouldy.Then, being difficult to judgement in visual inspection has or not mouldy in the situation that, adopt following method: utilize microorganism checking facility etc., upload and be set up the sample of stating collection at the mould yeast culture nutrient agar that is accommodated in culture dish, cultivate mould, the bacterium colony observe, instrumentation occurring on the 5th～7 afterwards, confirms to have or not mouldy.
But, utilize in macroscopic confirmation method above-mentioned, there is mould to check the have to problem of the experience that depends on operating personnel of precision.In addition, in the time cultivating mould, also must before the cultivation operation of mould, make nutrient agar with the facility that can carry out aseptic technique, nutrient agar is filled in to culture dish main body, operation is loaded down with trivial details.And, in the time that nutrient agar mounting checks sample, by with culture dish body portion from the lid operability of taking off the operation that maybe lid taking off is covered again to culture dish main body from culture dish poor, so in the situation that operating personnel's experience is still shallow, have the problem that needs more personnel in easy generation substratum pollution and mould Inspection.
For this reason, propose following method, for example, in the case of suspecting that mouldy sample is brown rice, detected the aroma component that mouldy brown rice has and identify mouldy brown rice (patent documentation 1).But, this recognition methods, except catching concentrated aroma component, also must be used gaschromatographic mass spectrometric analysis instrument or electric conductive polymer type sensor to detect, in addition, cause the problem of the apparatus and equipment that needs are expensive, and have mould to check that object is limited to the problem of brown rice.
Prior art document
Patent documentation
[patent documentation 1] Unexamined Patent 11-94821 communique
Summary of the invention
In view of the foregoing, the object of the present invention is to provide a kind of recognition methods of musty grain, the method does not rely on operating personnel's experience, can carry out accurately mould inspection, and mould Inspection is simple and easy, without special microorganism checking facility or apparatus and equipment.
The 1st scheme of the present invention is a kind of Simple recognition method of musty grain, it is characterized in that, described method comprises following operation: prepare the operation of mycotic culture container, described mycotic culture has storage mould with container and detects the resin lid with the resin main body of substratum and the peristome of the described resin main body of sealing; The operation of opening will be carried out by the peristome of the described resin main body of described resin lid sealing; Detected operation with substratum mounting cereal by the peristome of opening in described mould from described; With described by after the peristome of opening with described resin lid covering, the operation of at room temperature taking care of described mycotic culture container.
Mould detects uses substratum through sterilising treatment, is accommodated in mycotic culture with in the resin main body of container.Operating personnel opens the mycotic culture resin lid of container, by from suspecting that mouldy batch, optional multiple inspection are placed on predetermined distance the mould detection substratum that is accommodated in resin main body by more than.Then, again close described mycotic culture with after the described resin lid of container, at room temperature take care of described mycotic culture container.After a few days, each checks grain visual inspection, confirms that mould has or not growth.Then, for be placed in mould detect inspection grain that the confirmation mould that contains regulation ratio with the inspection grain of substratum grows batch, be judged as mouldy sample, implement the discarded disposal that waits regulation.It should be noted that, so-called " cereal ", refers to the farm crop of cultivating for edible seed in farm crop, for example, can enumerate the small-sized seeds such as meter Mai and coarse cereals class (coarse cereals herein refer to the farm crop except rice wheat).
The 2nd scheme of the present invention is a kind of simple and easy identifying schemes of musty grain, it is characterized in that, replace described resin lid, have described resin main body is accommodated in to inner resin storage box main body and the storage box cover that the peristome of described storage box main body is sealed.
In this scheme, the detection of storage mould is sterilely accommodated in resinous storage with in box by the resin main body of substratum, operating personnel opens storage box cover resin main body is taken out from storage box main body, or is being accommodated in storage with checking for mould under the state of box main body.
The 3rd scheme of the present invention is a kind of Simple recognition method of musty grain, it is characterized in that, described resin lid is film or the tabular body that is free to slide.In addition, the 4th scheme of the present invention is a kind of Simple recognition method of musty grain, it is characterized in that, described storage box cover is film or the tabular body that is free to slide.
In this scheme, when resin lid is film, the periphery of film is fixed in the top, periphery top of the resin main body of the peristome that forms resin main body, by the peristome sealing of resin main body.If peel off the film that covers peristome, the state of peristome in opening from the top, periphery top of resin main body.For example, by using the Continuous pressing device for stereo-pattern top, periphery top of adhering film and resin main body again, or use the fixing part that can be embedded in the periphery of film and the periphery of resin main body and push up the coincidence part on top, the peristome of opening can be closed again.
In addition, when resin lid is the tabular body being free to slide, the groove that tabular body is arranged at resin main body inner wall guides slidably, the peristome of covering resin main body processed and sealing.When the tabular body that makes to cover peristome slides to the direction parallel with the bottom surface of resin main body, the state of peristome in opening.By making tabular body slide into original position, the peristome of opening can be closed again.
The 5th scheme of the present invention is a kind of Simple recognition method of musty grain, it is characterized in that, described mould detection is nutrient agar with substratum, and the agar concentration of described nutrient agar is 1.15%～1.35%.The agar concentration of common nutrient agar is 1.5%～1.6%, with respect to this, in such scheme, agar concentration is set as to 1.15%～1.35%, makes it softer.
The 6th scheme of the present invention is a kind of Simple recognition method of musty grain, it is characterized in that, on the inner bottom surface of resin main body that is accommodated with described mould detection substratum, there is the separating part that the inside of described resin main body is divided under overlooking to multiple regions, in described each region through cutting apart, load a cereal.That is, in this scheme, the inner bottom surface of resin main body is separated part and is slit into multiple regions, at each region storage mould detection substratum.
The 7th scheme of the present invention is a kind of Simple recognition method of musty grain, it is characterized in that, the size of the short transverse of described separating part detects with the gauge of substratum large than described mould.; in this scheme; the mould detection substratum that is accommodated in each region does not contact with the mould detection substratum in the region that is accommodated in adjacency, and the mould that is accommodated in each region detects with substratum independent of one another with substratum with respect to the mould detection that is accommodated in other regions.
The 8th scheme of the present invention is a kind of simple and easy identifying schemes of musty grain, it is characterized in that, described resin main body is rectangle overlooking lower.
According to the 1st scheme of the present invention, owing to using the switching operation of carrying out peristome with the lid of main body complete installation, check grain at mycotic culture with placing in container, so check that the placement operation of grain is rapid and simple and easy.Thus, check the operability excellence of the placement operation of grain, so the substratum that can prevent from placing in operation pollutes.Therefore, the special apparatus and equipment without clean bench etc. for preventing that substratum from polluting, even if also can implement the recognition methods of musty grain in the cleaner room in storage warehouse or outside storage warehouse etc.
According to the 2nd scheme of the present invention, be accommodated with the mould detection resin main body of substratum and be accommodated in resinous storage with in box, so the resin main body breakage can prevent keeping mycotic culture container time.
According to the 3rd, the 4th scheme of the present invention, lid is film or the tabular body that is free to slide, so can be easily and promptly carry out the switching operations of peristome.
According to the 5th scheme of the present invention, by the agar concentration of nutrient agar is set as to 1.15%～1.35%, reaches and a part that checks grain can be imbedded to the hardness in substratum, check the position of grain on substratum so can fix.
According to the 6th scheme of the present invention, mould detects and is split into multiple regions with substratum, and 1 of each region mounting checks grain, so check that the location of grain on substratum is easy, can accelerate the Inspection of mould.In addition, each inspection grain is placed according to each region disconnecting, so even at the sample of arranging other kinds in container for identical mycotic culture, also easily carry out the identification judgement of sample.
According to the 7th scheme of the present invention, the size of the short transverse of separating part detects with the gauge of substratum large than mould, even so mycotic culture cannot maintain level with the keeping state of container, also can prevent from loading in the sample of specific region and move to other regions.In addition, because separating part detects and uses the face of substratum outstanding from mould, so the mould that can suppress to produce in the sample of regulation debouches other regions of adjacency.Therefore, can more correctly hold the ratio of mouldy inspection grain, and then also can the sample inspection with corresponding multiple kinds in container or multiple batches at mycotic culture.
According to the 8th scheme of the present invention, because resin main body is rectangle under overlooking, so compared with the resin main body that there is bend with culture dish etc., even if main body entire area is roughly the same, multiple regions with even area also can be set, can increase the placement quantity that checks grain.Thus, owing to checking object at a mycotic culture with increasing in container, so check that precision improves.In addition, owing to can making the area homogenizing in each region, so the mould that can suppress to grow to expanding in abutting connection with sample, thereby the mouldy sample of recognition and verification positively.
Brief description of the drawings
[Fig. 1] figure (a) is the orthographic plan for the mycotic culture container of the Simple recognition method of the present invention's the 1st embodiment example, and figure (b) is the sectional view for the mycotic culture container of the Simple recognition method of the present invention's the 1st embodiment example.
[Fig. 2] is the explanatory view of the opening operation of the peristome of the Simple recognition method of the present invention's the 1st embodiment example.
[Fig. 3] is the explanatory view of the sample mounting operation of the Simple recognition method of the present invention's the 1st embodiment example.
[Fig. 4] is the explanatory view of the operation of closing peristome of the Simple recognition method of the present invention's the 1st embodiment example.
[Fig. 5] is the explanatory view of the keeping operation of the Simple recognition method of the present invention's the 1st embodiment example.
[Fig. 6] is the figure that represents the mouldy state in the embodiment of the present invention.
[Fig. 7] figure (a) is the orthographic plan for the mycotic culture container of the Simple recognition method of the present invention's the 2nd embodiment example, and figure (b) is the sectional view for the mycotic culture container of the Simple recognition method of the present invention's the 2nd embodiment example.
Nomenclature
1 mycotic culture container
2 main bodys
3 lids
4 mould detection substratum
5 peristomes
7 separating parts
8 separating parts
11 mycotic culture containers
12 storage box main bodies
13 storage box covers
15 peristomes
Embodiment
Then, use the Simple recognition method of the musty grain of brief description of the drawings embodiment of the present invention example.
The 1st embodiment example of the present invention comprises following operation: prepare the operation of mycotic culture container, described mycotic culture has storage mould with container and detects by the resin main body of substratum with by the resin lid of the peristome sealing of described resin main body; By the operation of the peristome opening of the described resin main body being sealed by described resin lid; Detected operation with the substratum mounting grain of rice by the peristome of opening in described mould from described; By the described operation of again being closed described peristome by the peristome of opening of described resin lid covering; At room temperature take care of the operation that described mycotic culture container is cultivated mould.
The mycotic culture that uses in the 1st embodiment example of the present invention with container 1 as Fig. 1 (a) (b) as shown in, be made up of resinous main body 2 and the resin lid 3 of film like, described resin lid 3 has the shape corresponding with the plan view shape of main body 2.Main body 2 inside are filled with as the mould of nutrient agar and detect with substratum 4.Be located at the peripheries of peristome 5 sides of main body 2 towards 2 ground, outsides weeks of main body with the planar portions 6 of the bottom surface almost parallel of main body 2.The planar portions 6 of being located at peristome 5 week has roughly the same width.In addition, be located at a planar portions 6 of 2 planar portions 6 that are arranged in the direction vertical with respect to the length direction of main body 2 towards the lateral process of main body 2 with the flat part 9 of the rectangle of the bottom surface almost parallel of main body 2.And then the surface of flat part 9 is formed with 2 projections side-prominent to lid 3.
The lid 3 of film like is fixed in planar portions 6, thereby the peristome 5 of main body 2 is sealed.On the other hand, the lid 3 of film like is not fixed in flat part 9.Therefore, operating personnel utilizes described projection to grasp and is not fixed in after the part of lid 3 of flat part 9, and described part is stretched to the length direction of main body 2, can make thus lid 3 easily peel off from main body 2.
In addition, on the surface of lid 3, be provided with and charging to position 21 corresponding to the position of flat part 9.Charge to position 21 for example for recording the batch number of mycotic culture container 1 or the sample name of inspection grain 20 etc.Charging to position 21 herein, is that the surface that whitewash is coated to lid 3 obtains.It should be noted that, the size of the main body 2 of above-mentioned the 1st embodiment example comprises that planar portions 6 and flat part 9 are 125mm × 55mm, and the degree of depth is 10mm.
Detected and be sterilizing state with substratum 4 by the inside of the airtight main body 2 of lid 3 and the mould that is accommodated in main body 2 inside.Therefore, only otherwise lid 3 is peeled off from planar portions 6, mycotic culture detects the structure that pollution does not occur with substratum 4 with container 1 for main body 2 inside and mould.In addition, on the inner bottom surface of main body 2, equally spaced erect 4 separating parts 7 in the parallel direction of the length direction with respect to main body 2, in the vertical direction of the length direction with respect to main body 2, erect 4 separating parts 8, main body 2 inside are divided into 25 regions.The short transverse of described separating part 7,8 is of a size of below the depth dimensions of main body 2, and detects with the gauge of substratum 4 large than mould.
Mould detects with substratum 4 and from the substratum of cereal separating mould and yeast and calculating bacterium number, is not particularly limited as long as can be used for.Conventionally, the general substratum using is for example YM nutrient agar.In addition, preferred mould detects with in substratum 4, the separatory selection substratum of thread fungus based on as relevant to food decay or the calculating of yeast is applicable to, can suppresses the viewpoint that expansion property mould grows, can enumerate DRBC (dichloro mutter rose-bengal chloramphenicol, Dichloran-Rose-bengal-Chloramphenicol) nutrient agar; Calculate, can suppress based on being applicable to having a liking for dryness thread fungus from the food separation of dry or partial desiccation state the viewpoint that expansion property mould grows, can enumerate DG18 (dichloro mutter glycerine, Dichloran-Glycerol) nutrient agar; Using the productive flavus of aflatoxin (Aspergillus flavus), Aspergillus parasiticus (Aspergillus parasiticus) viewpoint of decouples computation bacterium number rapidly as mould poison, can enumerate AFPA (Aspergillus Flavus/Parasiticus Agar) substratum etc. based on being applicable to.
Operating personnel in the rice in storage warehouse, from suspect mouldy batch gathers a meter sample with carton in keeping, utilizes visual inspection to have or not mouldy.But, being difficult to judgement and having or not when mouldy, operating personnel takes the grain of rice gathering toward near cleaner room warehouse.Then, as shown in Figure 2, pick up the container 1 for mycotic culture of preparing in advance in room, peel off lid 3 by peristome 5 openings of sealing from planar portions 6 then and there.Now, can make lid 3 separate completely from main body 2, but be easy to viewpoint from the workability that makes following operation of closing peristome 5, preferably do not make lid 3 separate completely from main body 2, not give in mould and to detect by the degree of arranging the grain of rice on substratum 4 and bring obstacle, make a part for lid 3 be fixed in advance the planar portions 6 of main body 2.
Then, as shown in Figure 3, operating personnel takes the grain of rice collecting with carton with tweezers.Be, to check grain 20 with the grain of rice that these tweezers are taked.Then, operating personnel will check that grain 20 pushes gently mould and detects the surface with substratum 4, and a grain is arranged and is positioned over each region.Prevent each inspection grain 20 viewpoints of stirring from the bottom of each inspection grain 20 being imbedded to mould detection substratum 4 in mycotic culture container 1, mould detection is 1.15%～1.35% with the agar concentration of substratum 4, break and make to check that grain detects with the mounting operation substratum 4 and becomes and be easy to viewpoint in mould from positively preventing that mould from detecting with substratum 4, be preferably 1.20%～1.30%.
As shown in Figure 4, operating personnel loads and checks after grain 20 in 25 whole regions, and lid 3 is returned to the position before peristome 5 openings, again closes peristome 5, and described lid 3 does not separate completely from main body 2, makes a part be fixed in main body 2.The fixing means of the planar portions 6 of the periphery of lid 3 and main body 2 is not particularly limited, for example, can enumerate carry out with Continuous pressing device for stereo-pattern bonding, utilize fixing of installing that the fixing part of the coincidence part that can be fitted to the periphery of lid 3 and the planar portions 6 of main body 2 carries out etc.In embodiment example shown in Fig. 4, use fixing part 10.
Cultivate after 48～72 hours, 25 check grain 20 in, for example in the case of confirm more than 1/5 be less than half mould grow, be judged to be mouldy sample have doubtful, as required again implement test.25 check in grain 20, for example, in the situation that confirmation moulds more than half grow, be judged to be mouldy sample, the corresponding discarded disposal that waits regulation of batch enforcement.In addition, 25 check in grain 20, confirm that the inspection grain that mould grows is for example less than in 1/5 situation, is judged to be not mouldy sample.It should be noted that, the mycotic culture after use does not carry out sterilising treatment with container 1 under High Temperature High Pressure, for example, after being SYNTHETIC OPTICAL WHITNER dip treating with commercially available chlorine, can directly discard.
Then, the 2nd embodiment example of the present invention is described.It should be noted that, for the identical symbol of the constituent element mark identical with above-mentioned the 1st embodiment example.In the 1st embodiment example, use by being filled with mould and detect the container 1 for mycotic culture forming with resin main body 2 and the resins in film form lid 3 processed of substratum 4, but replace this container, in the 2nd embodiment example, as shown in Figure 7, use has the container 11 for mycotic culture of box cover 13 for the resin storage of resin storage box main body 12 and film like, described storage is accommodated in inside with box main body 12 by being filled with the mould detection resin main body 2 of substratum 4, described resin storage has the shape corresponding with the plan view shape of storage box main body 12 with box cover 13.
In the 2nd embodiment example, with storage by the planar portions 16 of the bottom surface almost parallel of box main body 12 towards the periphery of being located at peristome 15 sides of receiving use box main body 12 in 12 outside weeks of box main body for storage.The planar portions 16 of being located at peristome 15 week has roughly the same width.In addition, be located at a planar portions 16 of 2 planar portions 16 that are arranged in the direction vertical with respect to the length direction of storage box main body 12 towards the storage lateral process of box main body 12 with the flat part 19 of the rectangle of the bottom surface almost parallel of box main body 12 with storage.The storage of film like is fixed in planar portions 16 with box cover 13, and storage is sealed with the peristome 15 of box main body 12 thus.Therefore, the lid 3 of film like is not fixed in the planar portions 6 of main body 2.
In addition, as shown in Figure 7, storage is provided with 2 place's main body press sections 22 with box cover 13.Main body press section 22 is the rectangular protrusions that are arranged at storage main body 2 sides of box cover 13, is arranged on the position with the bight subtend of main body 2 sidewalls away from flat part 19.The bight that from top extruding, main body press section 22 is accommodated in to main body 2 sidewalls of storage box main body 12, main body 2 is supported in storage box main body 12 thus.Thereby prevent that main body 2 is in the interior movement of storage box main body 12.
The storage of film like is not fixed in flat part 19 with box cover 13.Therefore, operating personnel grasps the storage that is not fixed in flat part 19 with after the part of box cover 13, and described part is stretched with the length direction of box main body 12 to storage, can make thus storage box cover 13 easily peel off from storage box main body 12.
In addition, storage, with in box cover 13, is provided with and charges to position 21 in the position corresponding with flat part 19.Charge to position 21 for example for recording the batch number of mycotic culture container 11 or the sample name of inspection grain 20 etc.Charging to position 21 herein, coats storage by whitewash and obtains with box cover 13 surfaces.
The mould that is contained the inside of the storage box main body 12 airtight with box cover 13 and is accommodated in main body 2 inside detects and is sterilizing state with substratum 4.Therefore,, as long as storage is not peeled off from planar portions 16 with box cover 13, mycotic culture is that storage detects by the non-staining structure of substratum 4 with box main body 12 inside and mould with container 11.In addition, identical with above-mentioned the 1st embodiment example, at the inner bottom surface of main body 2, equally spaced erect 4 separating parts 7 in the parallel direction of the length direction with respect to main body 2, equally spaced erect 4 separating parts 8 in the vertical direction of the length direction with respect to main body 2, main body 2 inside are divided into 25 regions.The short transverse of described separating part 7,8 is of a size of below the storage depth dimensions of box main body 12, and detects with the gauge of substratum 4 large than mould.
Below, not diagram, illustrates the each operation that uses mycotic culture to use the 2nd embodiment example of container 11.First, peel off storage box cover 13 from planar portions 16, by peristome 15 openings of sealing.Now, in order to make the workability of the following operation of closing peristome 15 easy, preferably storage does not separate from storage box main body 12 completely with box cover 13, not give, the main body 2 of storage mould detection substratum 4 is taken out to the degree of bringing obstacle from mycotic culture container 11, make in advance one partial fixing be adhered to the planar portions 16 of storage box main body 12.
Then, operating personnel uses the space forming between box main body 12 to insert finger in flat part 9 and the storage of the main body 2 that is accommodated in storage box main body 12, grasps the flat part 9 of main body 2, and main body 2 is taken out with container 11 from mycotic culture.Then, detect the surface alignment inspection grain with substratum 4 in the mould of taking out.
Detect after the surface alignment inspection grain with substratum 4 in mould, main body 2 is returned to the original position in storage box main body 12, lid 13 is returned to original position, again close peristome 15, a part for described lid 3 is fixed in the storage box of storage box main body 12 in advance.Storage is not particularly limited with the fixing means of the planar portions 16 of storage box main body 12 with the periphery of box cover 13, but identical with the 1st embodiment example, for example can enumerate utilize that Continuous pressing device for stereo-pattern carries out bonding, utilize fixing of installing that the periphery that can be fitted to storage box cover 13 and the fixing part of the coincidence part of the planar portions 16 of storage box main body 12 carry out etc.By the keeping operation of container 11 and have or not mouldy decision method, identical with the 1st embodiment example about mycotic culture subsequently.
Then, other embodiment examples of the present invention are described.In above-mentioned the 1st embodiment example and the 2nd embodiment example, mycotic culture uses film like member with lid 3, the storage box cover 13 of container 1,11, lid 3, storage are fixed in the planar portions 6,16 of main body 2, storage box main body 12 with box cover 13, but replace this film like member, also can be following proposal, the tabular body that is set as being free to slide with box cover by lid, storage, makes lid, storage box cover slide, thereby carry out the switching operation of peristome 5,15.
In above-mentioned the 1st embodiment example and the 2nd embodiment example, check that object is rice, but be cereal, be not limited to rice, for example, also can be used for wheat, beans.
In above-mentioned the 1st embodiment example and the 2nd embodiment example, in all substratum in 25 regions of main body 2, load inspection object of the same race, but for example also can load and different types of inspection object such as rice and beans.In addition, the number of regions that is arranged at main body 2 is not limited to 25, can suitably change number of regions by the number that changes separating part 7,8.And then, in above-mentioned the 1st embodiment example and the 2nd embodiment example, each part that checks grain 20 is imbedded to mould detection substratum 4, but can not imbed mould detects with substratum 4, only inspection grain 20 is loaded in its surface, now, improve mould and detect the agar concentration with substratum 4, mould detection is set as higher than above-mentioned the 1st embodiment example and the 2nd embodiment example by the hardness of substratum 4.
In addition, in above-mentioned the 1st embodiment example and the 2nd embodiment example, charging to position 21 is to obtain at lid 3, the storage surface coated whitewash of box cover 13, but replace this to charge to position, also described surface working can be become to concavo-convex, the position of charging to frosted shape outward appearance, can also not arrange and charge to position as required.And then, in above-mentioned the 1st embodiment example and the 2nd embodiment example, flat part 9,19 being set, also can suitably omit.In addition, in above-mentioned the 2nd embodiment example, main body 2 is taken out and arranged inspection grain with container 11 from mycotic culture, but replace the method, also main body 2 can be accommodated in to mycotic culture with arranging inspection grain under the state of container 11.
[embodiment]
Then, further illustrate the present invention by embodiment, the present invention is not limited to these disclosed items certainly.
(embodiment 1)
.
the mycotic culture container using: the mycotic culture container shown in Fig. 1
, use and comprise that being accommodated with mould detects the container with the film like lid that the peristome of this main body is sealed by the main body of substratum.
.
the mould detection substratum using: DRBC nutrient agar
Composition
Glucose ... ..10.0g/L
Peptone ... ... 5.0g/L
Potassium primary phosphate ... 1.0g/L
Magnesium sulfate ... ..0.5g/L
Dichloro is muttered ... 0.002g/L
Rose-red ... .0.025g/L
Paraxin ... 0.100g/L
Agar ... ... .13.0g/L
Purified Water ... ... .1.0L
pH?5.6±0.2
About modulator approach
After heating for dissolving, autoclaving 15 minutes at 121 DEG C, modulation DRBC nutrient agar.
After sterilizing, naturally cool to after 50 DEG C, fully stir DRBC nutrient agar, be dispensed into 25 regions that the mycotic culture after sterilizing has with container.Now, point fluence in each region is to make the gauge of DRBC nutrient agar less than the size of the short transverse of the separating part in 25 regions of formation.After dispensing, film like lid sealed open portion for method according to the rules, makes above-mentioned mycotic culture container.Prepare this mycotic culture container in advance, keeping is at the site of storage of regulation, can use at once when needed.
Keeping in the storage polished rice of storage, for suspecting mouldy batch, a part that uses carton to gather batch.By the polished rice grain on visual inspection carton, confirm to have or not mouldy.Then, be difficult to judgement for detecting by an unaided eye and have or not mouldy sample, mouldy in order to use mycotic culture container to judge to have or not, the polished rice grain on carton is transferred to cleaner room from storage.Then, the mycotic culture of taking care of in room is in advance peeled off from main body with the lid of container, thereby by the peristome opening of the mycotic culture use container of sealing.After opening, the polished rice grain from carton is selected 25 arbitrarily, is arranged in respectively the mould detection substratum that is divided into 25 regions of above-mentioned mycotic culture container.Then, installation can be embedded in the parts of the coincidence part of the periphery of lid and the planar portions of main body, thereby again closes peristome with described lid.Then, mycotic culture is at room temperature taken care of 72 hours with container, cultivated mould.The results are shown in Fig. 6.
(embodiment 2)
Except using YM substratum shown below to detect with substratum as mould, use the mycotic culture of the test conditions identical with embodiment 1 and batch enforcement polished rice grain.
.
the mould detection substratum using: YM substratum
Composition
Glucose ... ..15.0g/L
Soytone (ソ イ ト Application, soya peptone) ... ... 6.0g/L
Malt extract ... ..5.0g/L
Yeast extract paste ... ..1.0g/L
Agar ... ... .16.0g/L
Rose-red, Streptomycin sulphate, paraxin suitably add
Purified Water ... ... .1L
About modulator approach
After heating for dissolving, autoclaving 15 minutes at 121 DEG C, modulation YM substratum.
Not diagram, in embodiment 2, is arranged in respectively amounting in 25 polished rice grains of 25 regions in above-mentioned mycotic culture container, can observe 8 mouldy.
(comparative example), except the agar addition in DRBC agar basic medium is set as the 15.0g/L of existing composition, makes the identical DRBC nutrient agar of substratum using with embodiment 1.By this DRBC nutrient agar autoclaving 15 minutes at 121 DEG C.After sterilizing, DRBC nutrient agar is naturally cooled to after 50 DEG C, fully stir, dispensing is in the culture dish of 5 sterilizings.In comparative example, use 5 culture dish of having filled this DRBC nutrient agar as mycotic culture container.In cleaner room, for each 5 culture dish, take off the lid of culture dish, in the polished rice grain from carton, select respectively arbitrarily 5 in the same manner with embodiment, on substratum, arrange polished rice grain, while making visual inspection, be the interval of approximate equality.Then,, in culture dish body cover upper cover body, at room temperature take care of 72 hours in the same manner with embodiment.
It should be noted that, in comparative example, the reason that is arranged in a polished rice grain in culture dish and is set as 5 is: 1) culture dish is spherical under overlooking, cannot arrange more polished rice grain at substratum periphery in order to ensure the distance of polished rice intergranular; 2) inner bottom surface of culture dish not by multiple partitioned portions every, arrive other polished rice grains of adjacency and must fully guarantee the distance of above-mentioned polished rice intergranular in order to suppress the mould of growth.Arrange under more than 6 states at 1 culture dish, when mouldy on the polished rice grain of regulation, the mould of growth easily arrives other polished rice grains of adjacency, cannot judge the ratio of mouldy polished rice grain.
In embodiment 1, as shown in Figure 6, in 25 polished rice grains, observe 9 mouldy, in embodiment 2, in 25 polished rice grains, observe 8 mouldy.That is, in embodiment 1,2, confirm more than 1/5 to be less than half mould and grow, so in any situation of embodiment 1,2, batch can be judged as after sampling with carton is doubtful mouldy.In addition, in any of embodiment 1,2, mould detection is showed no to having or not mouldy judgement to bring difficult substratum to pollute with substratum, can judge exactly.Think that reason is to utilize rapidly the switching operations of the peristome of lid.
On the other hand, not diagram, comparative example is identical with embodiment batch, observes 4 culture dish and 1 funginert culture dish that polished rice grain is mouldy.Therefore, clear and definite in comparative example only otherwise increase the number of culture dish, just cannot guarantee to have or not mouldy inspection precision, during with 1 culture dish, compare with container with the mycotic culture of 1 embodiment, inspection low precision.In addition, in comparative example, visible substratum pollutes, and compared with embodiment, has or not mouldy judgement more difficult as the polished rice grain that checks grain.And then, in comparative example, be difficult to measure mouldy polished rice grain number because substratum pollutes, all cannot judge whether to be judged to be doubtful mouldy batch, still should be judged to be mouldy sample batch.Think that reason is the switching operations of the peristome that can not promptly utilize lid or the operation of arranging polished rice grain.
Utilizability in industry
The present invention can in the case of do not rely on operating personnel's experience and do not use simple and easy special instrument and precision confirm well to have or not mouldy, so utility value is high in the field of the mouldy storage cereal of identification.
Claims (4)
1. a recognition methods for musty grain, is characterized in that, comprises following operation:
Prepare the operation of mycotic culture container, described mycotic culture has with container: storage mould detects the resin main body with substratum, on the inner bottom surface of described resin main body, comprise the inside of described resin main body is divided into size multiple regions, short transverse than the large cutting part of gauge of described mould detection substratum under overlooking; With resins in film form lid processed, it is by being close to the edge of peristome side of described resin main body, in the position of the peristome facing to described resin main body, by the peristome sealing of described resin main body;
Peel off described resin lid from described resin main body, by the operation of the peristome opening of the described resin main body being sealed by described resin lid;
Detected operation with substratum mounting cereal by the peristome of opening in described mould from described; With
Described by after the peristome of opening with described resin lid covering, the operation of at room temperature taking care of described mycotic culture container;
Also comprise the steps:
At a described cereal of regional mounting through cutting apart;
Detecting by an unaided eye, each checks grain, confirms that mould has or not growth; With
For be placed in mould detect contain regulation ratio with the inspection grain of substratum confirmation mould grow inspection grain batch, be judged as mouldy sample.
2. the recognition methods of musty grain as claimed in claim 1, is characterized in that, replaces the resin lid of described film like, comprises the tabular body being free to slide.
3. the recognition methods of musty grain as claimed in claim 1, is characterized in that, described mould detection is nutrient agar with substratum, and the agar concentration of described nutrient agar is 1.15%～1.35%.
4. the recognition methods of musty grain as claimed in claim 1, is characterized in that, described resin main body is rectangle overlooking lower.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009250836A JP4787900B2 (en) | 2009-10-30 | 2009-10-30 | Simple identification method for moldy grain |
| JP2009-250836 | 2009-10-30 |
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| Publication Number | Publication Date |
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| CN102051403A CN102051403A (en) | 2011-05-11 |
| CN102051403B true CN102051403B (en) | 2014-06-25 |
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| CN201010533845.9A Expired - Fee Related CN102051403B (en) | 2009-10-30 | 2010-10-29 | Simple recognition method of mildew cereal |
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| CN (1) | CN102051403B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201232056Y (en) * | 2008-04-15 | 2009-05-06 | 李荣春 | Multipurpose biological culture box |
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|---|---|---|---|---|
| DE3028204A1 (en) * | 1980-07-25 | 1982-02-25 | Biotest-Serum-Institut Gmbh, 6000 Frankfurt | FEDERAL FLOOR SUPPORT SYSTEM |
| JP2000116399A (en) * | 1998-10-14 | 2000-04-25 | Eiken Chem Co Ltd | Fractionation medium for discrimination between drug sensitive bacillus and drug resistant one, and discrimination used therein |
| DE102006044546A1 (en) * | 2006-09-22 | 2008-04-03 | Biotest Ag | Air Sampler |
| DE102006044547B4 (en) * | 2006-09-22 | 2009-03-19 | Biotest Ag | Air sampler, collecting strip therefor and method for inserting the collecting strip |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201232056Y (en) * | 2008-04-15 | 2009-05-06 | 李荣春 | Multipurpose biological culture box |
Non-Patent Citations (4)
| Title |
|---|
| 《Mould contamination on rice》;Masakatsu Ichince;《Mycotoxins》;19941231;第40卷;11-14 * |
| 《饲料中霉菌盒霉菌毒素检测及关键问题探讨》;高慧 等;《饲料检测与品质控制》;20071231(第3期);17-19 * |
| Masakatsu Ichince.《Mould contamination on rice》.《Mycotoxins》.1994,第40卷11-14. |
| 高慧 等.《饲料中霉菌盒霉菌毒素检测及关键问题探讨》.《饲料检测与品质控制》.2007,(第3期),17-19. |
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| Publication number | Publication date |
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| CN102051403A (en) | 2011-05-11 |
| JP2011092133A (en) | 2011-05-12 |
| JP4787900B2 (en) | 2011-10-05 |
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Address after: Tokyo, Japan, Japan Applicant after: JAPAN GRAIN INSPECTION ASSOCIATION Co-applicant after: Oiles Industry Co., Ltd. Address before: Tokyo, Japan, Japan Applicant before: Japan Grain Inspection Association Co-applicant before: Oiles Industry Co., Ltd. |
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