JP2011001350A - Bathing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new bathing agent developed by paying attention to Candida antibodies against Candida as a foreign invader and utilizing the Candida antibodies.SOLUTION: The bathing agent comprises at least either one of a principal bathing ingredient and an auxiliary bathing ingredient and an egg yolk antibody of Immunoglobulin Yolk (IgY). The IgY includes Candida antibodies (IgYCA) in which Candida is an antigen. This agent contains the IgYCA in the range of 0.01-99.99%, which makes it possible to protect the skin from Candida.

Description

  The present invention relates to a bath preparation to which a Candida antibody having Candida as an antigen is added.

Bathing agents are widely used, from those that stimulate the circulation of the blood by giving moderate irritation to the skin, improving fatigue and metabolism, and those that give a relaxing feeling by giving aroma and color to the bath water. ing.
Carbon dioxide dissolved in bath water has the effect of permeating into the skin and expanding peripheral blood vessels to promote blood circulation, heat retention effect, refreshing feeling imparting effect, etc. Tablet-type and briquette-type foaming bath agents that generate carbon dioxide gas by dissolving in hot water are also commercially available.

On the other hand, animals have a biological defense mechanism called immunity to protect themselves from external enemies such as bacteria and viruses that enter the body from the outside. A system that considers external enemies that have entered the body as "antigens" and produces proteins called "antibodies" in the blood and mucous membranes. The antibodies produced in the body detoxify or inactivate antigens that are enemies. And eliminate it outside the body. Antibodies are called immunoglobulins (Immunoglobulins: Ig) and are classified as follows according to their physicochemical properties and functions.
Many mammals have immunoglobulin G (hereinafter referred to as IgG), immunoglobulin M (hereinafter referred to as IgM), immunoglobulin D (hereinafter referred to as IgD), and immunoglobulin A (hereinafter referred to as “IgD”) due to differences in constant regions. IgA) and immunoglobulin E (hereinafter referred to as IgE). Among them, IgG accounts for 70-75% of human immunoglobulins, is the most abundant in blood, is averagely distributed in and out of blood vessels, and is the only isotype that can pass through the human placenta. IgM is human immunoglobulin It is usually present only in blood, is produced first against infectious microorganisms, is responsible for initial immunity, and is the largest antibody in humans, so it is difficult to spread and most of it is serum IgA accounts for 10-15% of human immunoglobulins, is a major role in mucosal immunity, functions as the front line of the immune system in the digestive and respiratory tracts, and IgD is less than about 1% of human immunoglobulins It is present on the surface of immature B cells, is involved in the induction of antibody production, is one of the antibody proteins, exists in a trace amount as a secreted protein in serum, and IgE is a human immunoglobulin. A glycoprotein 0 0.001% it does not only exist following, in one of the central role molecules in allergic reactions believed to be involved in the immune response against the parasite.
A major feature of antibodies is the specificity that a single antibody reacts only with a specific antigen. The binding force of an antigen-antibody reaction is so strong that it can be compared to the “relationship between key and keyhole”, and it is not easily separated.

Chickens have three types of antibodies: IgG, IgM, and IgA. The chickens transfer the antibodies of the mother chicken to the egg yolks and egg whites of the eggs that will be born, and then to the chicks that are children. The antibody transferred to the egg and the antibody taken out from the egg are called chicken egg antibody = Immunoglobulin York, and expressed as IgY. In the chicken egg antibody (hereinafter referred to as IgY), in particular, IgG in the blood is selectively transferred and accumulated only in the yolk, and the antibody extracted from the egg yolk is called an egg yolk antibody and “libetin”.
Functional food materials and quasi-drugs to prevent oral infection by applying IgY are produced by Gen Corporation (839-1 Sano, Gifu City, Gifu Prefecture) and trade name: Ovalgen / Ovalgen Sold.
For example, as shown in Figure 2, (1) Helicobacter pylori is said to affect more than 70% of Japanese people over the age of 40, affecting gastritis, stomach / duodenal ulcers, and stomach cancer. Based on the research result that urease in the outer membrane of Helicobacter pylori has strong affinity for sulfated gastric mucin under acidic conditions, oral passive immunotherapy using anti-urease IgY was developed, and H. pylori antibody It is added to yogurt as IgYHP (trade name: Ovalgen: HP), eaten, added to beverages, and drunk.
(2) IgYDC (trade name: Ovalgen: DC) has been developed as IgY against Streptococcus mutans, which is a cause of dental caries.
(3) IgYPG (trade name: Ovalgen: PG) has been developed and added to gum as IgY against Porphyromonas gingivalis which is the cause of periodontal bacteria.
(4) IgYCA (trade name: Ovalgen: CA) has been developed as IgY for Candida alpicans, the causative bacterium of oral candidiasis.
These IgYs are attracting attention as a safe and effective material for the human body because eggs are the raw material, and no side effects have been observed even during long-term ingestion in the human body.

On the other hand, vaginal candida is known as a case specific to women. It is said that this symptom caused by vaginal candida is experienced by one in five women, and it is known that it often recurs even after being treated.
Candida is a kind of mold, and it is known that there are yeast type and mycelium type as shown in FIG. 5, and it is known to change from yeast type to mycelium type, and the body (for example, oral cavity, intestine) and skin ( It is a resident bacteria that exists in the anus and its surroundings (including the genitals). In general, there is no difference between males and females because they reach the anus from the intestine and are implanted in and around the anus. Vaginal candita is often transmitted from the anus. In addition, if the amount is small and healthy, there is no harm, but resistance is weakened by fatigue, stress, etc., and it is said that if the pH of the skin changes, for example, the bacteria will grow easily.

Japanese Patent No. 2598802 Japanese Patent No. 3995541 JP 2008-247750 A JP 2007-82469 A JP 2006-36661 A

It is known that bathing in bath water warms the body temperature, expands skin tissue and blood vessels, and improves metabolism and blood circulation. In addition, it is known that when bath agents are added to the bath water, their effects are further improved.
On the other hand, IgY (including IgYCA) has been used as food and drink, and has only been considered for use as oral passive immunity.
Therefore, the present invention has been made in view of such problems of the prior art, and the inventors have focused on Candida antibodies that use Candida bacteria as an antigen. It came to invent the new bath preparation which can protect.

To achieve the above object, the bath preparation of the present invention comprises, as claimed in claim 1, at least one of a main component for bath and an auxiliary component for bath, and an egg yolk antibody (IgY) of an immunoglobulin yolk against the skin. The chicken egg antibody (IgY) is a Candida antibody (IgYCA) having Candida as an antigen.
A second aspect of the present invention is the bath agent according to the first aspect, wherein IgYCA is contained in a range of 0.01 to 99.99%.
A third aspect of the present invention is the bath agent according to the first or second aspect, wherein at least one of a moisturizing component, a hygroscopic component, a scent component, a coloring component and the like is used as a bath auxiliary component.
[Claim 4] In the bath preparation according to claim 1, 2 or 3, any one of an inorganic salt component, a carbon dioxide component, a medicinal plant component, and an enzyme component is used as a main component for bath. It is characterized by.

Here, chicken egg antibody (IgY) means that when the mother chicken is inoculated with an antigen, an antibody against the antigen is produced in the body, the antibody is transferred to the egg yolk and egg white of the egg to be born, and removed from the laid egg Say antibody.
Candida antibody (hereinafter referred to as “IgYCA”) refers to an antibody obtained by inoculating a mother chicken with Candida as an antigen, produced as IgYCA against the Candida, transferred to a laying egg, and extracted from the laid egg. In other words, the range that can be adopted as the content is 0.01 to 99.99%, and it is desirable to add at least 1.0% or more in the bathing agent used for 200 liters of bath water.

Here, Candida is a fungus, a so-called mold, and a typical bacterium is Candida albicans, which is normally present in the digestive tract and skin of the oral cavity and intestine and is usually harmful. There is nothing. However, resistance decreases due to fatigue, etc., immunity decreases due to certain drugs (immunoregulators, etc.), high temperature and humidity weather conditions, underwear made of synthetic fibers, etc. overlaps with tightness and poor hygiene And cause infections. The vagina, the genital area, the anus and its surroundings, the armpits, the sagging part of the abdomen, and the folds of the skin (between fingers and toes) are also susceptible to infection.
Here, the main component for bath means an inorganic salt component, a carbon dioxide component, a medicinal plant component and an enzyme component that are conventionally used for bathing agents, and a bath auxiliary component is conventionally used for bathing agents. It refers to moisturizing components, hygroscopic components, scent components, coloring components, and the like.
Here, the bathing agent refers to a powder state other than a liquid, a tablet state, or a solid state.

Here, the inside of the hot water refers to hot water in which the bathing agent of the present invention is put into the bathing water and the bathing agent is dissolved almost evenly, and “in the bathing water” is synonymous with “inside the bathing water”.
The term “floating” as used herein refers to a state in which Candida bacteria that have inhabited the human skin float in the bath water away from the skin when bathing in the bath water.
Here, adhering to Candida bacteria is a state that adheres to Candida bacteria that inhabit the skin, especially yeast-shaped Candida bacteria, and adheres to Candida bacteria that float and disperse in bath water, so that floating Candida bacteria gather, Moreover, it refers to a state of attachment with an extremely strong binding force, and the activity of Candida is suppressed. When IgYCA is attached to Candida, the entire volume increases. As a result, it means that the activity becomes significantly inferior compared to when alone, and killing Candida is completely covered with IgYCA adhering to Candida and blocked from the outside, detoxified, Say to inactivate.

Since the bath preparation according to the present invention is as described above, the following effects are obtained.
When the bath agent of claim 1 is introduced into the bath water, a Candida antibody (hereinafter referred to as IgYCA) having Candida as an external enemy dissolves and spreads over the bath water together with auxiliary components for bath. Moreover, even if IgYCA is added, it is easy to dissolve in bath water, and there is no discomfort even when bathing.
Because skin tissue and blood vessels by bathing expands, the Candida fungus that existed on the skin combined with IgYCA melted in the bath water as predators, inactivated or detoxified the Candida fungus, increases the volume. On the other hand, as a result of the body movement in the bath water and the movement, the bath water is agitated and the bath water flows. As a result, Candida bacteria having an increased volume are easily removed. That is, IgYCA effectively acts on Candida bacteria in the bathed skin. Moreover, since it is simply poured into the bath water as in the conventional bath preparation and bathed in the bath water, it can be used very easily and without the hassle of being a coating-type Candida remedy.
IgYCA has an advantage that it can be obtained at low cost since egg is a raw material. Moreover, since IgY is already used as a functional food material or quasi drug, it is safe.

In addition to the features of claim 1, the bath agent of claim 2 can be expected to have a certain effect by adding 0.01 to 99.99% IgYCA, but to maintain the same bathing effect as before. Is preferably added in the range of 1.0 to 10%.
Specifically, by containing 1.0% IgYCA, that is, 1.0% IgYCA with respect to the auxiliary bath component, or 1.0% IgYCA with respect to the inorganic salt-based component. It has been confirmed in experimental examples that the bath agent of the present invention used is effective even with 200 liters of hot water.
IgYCA also adheres to Candida bacteria floating in the bath water, and collects the floating Candida bacteria to increase the volume. As a result, the activity of Candida fungus floating in the bath water can be suppressed or killed.

In addition to the features of claims 1 and 2, the bath agent of claim 3 uses at least one of a moisturizing component, a hygroscopic component, a scent component, a coloring component, etc. as a bath auxiliary component. You can get the same moisturizing effect and enjoy the color and scent.
In addition to the features of claims 1, 2, and 3, the bath agent according to claim 4 includes any one of an inorganic salt component, a carbon dioxide component, a medicinal plant component, and an enzyme component as a main component for bath. Since it is used, effects equivalent to those of conventional bath preparations, for example, blood circulation promotion and the like are enhanced, and an excellent warm bath effect is obtained.

It is a block diagram which shows the component of the bath agent concerning this invention. It is a block diagram which shows the kind of chicken egg antibody used for the bath agent of this invention. It is a block diagram which shows the component example of the auxiliary component for baths used for the bath agent of this invention. It is a block diagram which shows the example of a component of the main component for baths used for the bath agent of this invention. It is a microscope picture which shows the yeast form and mycelium form of Candida. It is a comparative photograph in the culture medium which shows the influence of the bath preparation of this invention. It is a comparative photograph in 3 hours after a process of this invention bath agent. It is a comparative photograph in the 5th hour after processing of this invention bath agent.

The first embodiment of the bath preparation according to the present invention will be described with reference to FIG. 1. The bath egg supplement (S IgY) is contained at a ratio of 1.0% with respect to the bath auxiliary component S. As auxiliary component S, it contains at least one or more selected from moisturizing component S1, moisture absorbing component S2, scent component S3, coloring component S4, etc., specifically, moisturizing component S1 and scent component S3, and IgY Candida bacteria and a Candida antibody (hereinafter referred to as IgYCA) containing Candida bacteria as an antigen are contained.
IgYCA is produced in the same manner as IgYHP manufactured by Gen Corporation, which first inoculates chickens with Candida as an antigen, thereby producing Candida antibodies, ie IgYCA, in chickens. In this case, IgYCA is transferred to eggs to be laid and IgYCA is collected from the laid eggs.

When the bath agent of the present invention is added to the bath water, the IgYCA, the moisturizing component S1 and the scent component S3 are dissolved and spread in the bath water. When a human enters the bath water containing the bath preparation of the present invention, IgYCA adheres to the candida bacteria present on the human skin, covering the candida bacteria, detoxifying and inactivating (killing), but from the skin It also adheres to Candida bacteria that are dispersed and suspended in the bath water, and the suspended Candida bacteria are collected to form a larger aggregate (volume increases) than that at the time of dispersion.
The mechanism by which IgYCA adheres to Candida that exists in human skin is because IgYCA is an antibody against Candida. However, although the mechanism for covering Candida is unknown, for example, the skin tissue expands due to the bath temperature (about 40 ° C.), the movement of Candida becomes active due to the bath temperature, to counter IgYCA. Etc. are considered.
Then, bath water is swirled by moving limbs and body in the bathing state, resulting in flow and vortex in the bath water. As a result, Candida bacteria whose volume has increased due to adhesion of IgYCA is removed from the skin by the fluid pressure of the bath water. .

  If the second embodiment of the bath agent according to the present invention is described in terms of differences from the first embodiment, the bath agent of the second embodiment uses the main component M for bath instead of the auxiliary bath component S. The main component M for bath contains an inorganic salt component M1, a carbon dioxide component M2, a medicinal plant (herbal medicine) component M3, an enzyme component M4, for example, an inorganic salt component M1, IgYCA is used at a ratio of 1.0% with respect to the inorganic salt component M1.

The third embodiment of the bathing agent according to the present invention will be described in terms of differences from the first and second embodiments. The bathing agent according to the third embodiment includes a bath auxiliary component S and a bath main component M as shown in FIG. , And IgYCA are used.
The content of IgYCA can be employed in the range of 0.01 to 99.99% with respect to the bath agent (the auxiliary component S for bath and the main component M for bath), but in order to maintain the same bathing effect as before, It is desirable to add in the range of 1.0 to 10%. It has been confirmed in an experimental example that a content of 1.0% IgYCA is effective even with 200 liters of hot water.

  The main component M for bath used in the bath agent of the present invention is any one of inorganic salt component M1, carbon dioxide component M2, medicinal plant component M3 and enzyme component M4 as shown in FIG. Examples of the inorganic salt component M1 include chlorides such as sodium chloride, potassium chloride, and ammonium chloride; sulfates such as sodium sulfate, aluminum sulfate, iron sulfate, sodium thiosulfate, potassium thiosulfate, and sodium hyposulfite; sodium nitrate, Nitrates such as potassium nitrate and calcium nitrate; phosphates such as sodium phosphate, sodium polyphosphate and calcium hydrogen phosphate; sulfides such as sulfur, sodium sulfide, potassium sulfide and iron sulfite; sodium hydroxide and calcium hydroxide Hydroxide, borax, boric acid, calcium oxide, potassium bromide, potassium permanganate, etc. Used as the enzyme system components M4, for example, trypsin, alpha-chymotrypsin, bromelain, papain, protease, proctase, serratiopeptidase, lysozyme or the like is used.

  The bath auxiliary component S used in the bath agent of the present invention is at least one of a moisturizing component S1, a hygroscopic component S2, a scented component S3, a coloring component S4, etc. as shown in FIG. Plant extracts such as peach, loofah and aloe, vegetable extracts such as tomato, propylene glycol, 1,3-butylene glycol, glycerin, urea, sorbitol, glucose, sucrose, xylose and the like are used.

Further, as the scent component S3, for example, a fragrance such as lavender oil, jasmine oil, lemon oil; an artificial fragrance such as geraniol, citronellal, eugenol, undecalactone, limonene, phenethyl alcohol; The resulting blended fragrance is used.
Examples of the coloring component S4 include blue No. 1, red No. 106, red No. 2, yellow No. 4, yellow No. 202 (1), green No. 3, etc. In addition, a coloring matter recognized as a food additive such as Annat is used.

The bath agent of the present invention is produced using the following components.
As IgYCA, the trade name “Ovalgen CA” manufactured by Gen Corporation, as the main component M for bath, sodium bicarbonate and dry sodium sulfate as auxiliary component S for bath, silicic anhydride, sea salt, glycyrrhizin Dipotassium acid, orange oil, riboflavin and the like are used in amounts as shown in Table 1.

Test 1: Method and Results 1) Candida albicans (TIMM strain), a type of Candida albicans, was cultured in Roswell Park Memorial Institute (RPMI) medium supplemented with 5% Fetal bovine serum (FBS) 2) Bathing after 4 hours 3) Observation with a microscope every 30 minutes, comparing the length of mycelium shape Results: Bath treatment solution treatment group (upper) as shown in FIG. ) And the untreated group (below) were small.
Conclusion: There seems to be no effect on the formation of mycelial candida in the bath treatment group.
Discussion: The therapeutic effect on candita infection is thought to be low.

Test 2: Inhibition of adhesion to epithelial cells 1) Culture of FADU cells, which are a type of epithelial cells, in a sheet 2) Mix TIMM strain (10 ⁸ / ml) and bath solution (30 g / 200 liters) The equivalent concentration) is allowed to react for 1 hour.
3) In addition to cells, incubate for 1 hour.
4) Wash free Candida bacteria with PBS like physiological saline, and count Candida bacteria attached to the cells.
The results are shown in Table 2.

At 3 hours after treatment, the results are as shown in the test group (upper) and the control group (lower) in FIG.
At 5 hours after the treatment, the results and discussion are as shown in the test group (top) and control group (bottom) of FIG. 8. 1) The number of candida adhering in the bath treatment group was significantly reduced.
2) It is considered that the bath agent of the present invention has a preventive effect against candidiasis.

Test 3: Storage stability test Method: The antibody activity of the bathing agent of the present invention during storage is measured and compared with a standard antibody titer.
Results: No antibody degradation was observed at the end of 3 months.

  In principle, one bath agent of the present invention is used for one bathing, but the overall weight is formed to 60 g or less, and a plurality of bathing agents can be used for one bathing. It is also possible to form a weight of 60 g or more and divide it into multiple baths. Moreover, bathing agents having different colors can be provided by changing the coloring component S4, and bathing agents having different scents can be provided by selecting the scenting component S3.

The mixing ratio of IgYCA with respect to the bath auxiliary component S and the mixing ratio of IgYCA with respect to the bath main component M are not limited to 1.0%. It may be added in the range of 01 to 99.99%.
In order to maintain the same bathing effect as before, it is desirable to add IgYCA in the range of 1.0 to 10%.
IgYCA is effective up to 60 ° C., and the effect becomes weaker as the temperature becomes higher. However, IgYCA is most effective at 35 to 40 ° C. and is therefore optimal as a bath preparation.
Causes (mechanisms) of Candida spp.

IgY Chicken egg antibody IgYCA Candida antibody IgYHP Helicobacter pylori antibody IgYDC Streptococcus mutans antibody IgYPG Polyphyromonas gingivalis (periodontal antibody)
S Bath auxiliary component S1 Moisturizing component, S2 Hygroscopic component, S3 Fragrance component, S4 Coloring component M Bath main component M1 Inorganic salt component, M2 Carbon dioxide component M3 Medicinal plant (herbal medicine) component, M4 Enzyme component

Claims (4)

Candida antibody (IgYCA) consisting of at least one of a main component for bath and auxiliary component for bath, and a chicken egg antibody (IgY) of immunoglobulin yolk against the skin. Bathing agent characterized by being. The bathing agent according to claim 1, which contains IgYCA in a range of 0.01 to 99.99%. 3. The bath agent according to claim 1, wherein at least one of a moisturizing component, a hygroscopic component, a scent component, a coloring component and the like is used as a bath auxiliary component. The bathing agent according to claim 1, 2 or 3, wherein any one of an inorganic salt component, a carbon dioxide component, a medicinal plant component and an enzyme component is used as a main component for bathing. Agent.