JP2011001327A - INHIBITOR ON INCREASE IN STEM CELL PROLIFERATION FACTOR mRNA EXPRESSION - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find out, from among highly safe natural products, one exhibiting an inhibiting action on increase in stem cell proliferation factor (SCF) expression and to provide an inhibitor on increase in the stem cell proliferation factor (SCF) expression comprising the same as an effective ingredient.SOLUTION: The inhibitor on increase in the stem cell proliferation factor (SCF) expression includes as effective ingredients one or two or more selected from the group consisting of an extract of Equisetum arvense, an extract of Nasturtium officinale, an extract of Matricaria chamomilla and an extract of Swertia japonica.

Description

  The present invention relates to a stem cell growth factor mRNA expression increase inhibitor.

  Spots, buckwheat, skin pigmentation after sunburn, etc. occur as a result of markedly increased melanin production due to activation of pigment cells (melanocytes) present in the skin. It is connected. Conventionally, various therapeutic agents, external preparations for skin, cosmetics and the like are known as having a whitening effect. For example, as a compound that inhibits tyrosinase activity and suppresses melanin production, sulfur compounds represented by colloidal sulfur and glutathione are used. In addition, ascorbic acids, hydrogen peroxide, hydroquinone, catechol and the like are used to lightly bleach the produced melanin.

  However, ascorbic acids are unstable when they are contained in water-rich cosmetics such as water-containing cosmetics, so they are unstable and cause discoloration of the cosmetics. Further, the hydrogen peroxide solution has problems in storage stability and safety in use. Furthermore, sulfur compounds such as glutathione and colloidal sulfur have a problem in that they emit a significant off-flavor. Furthermore, hydroquinone and catechol have skin irritation and allergic properties and have a problem in safety in use. Accordingly, the use of these whitening ingredients in products such as cosmetics is restricted, and there are no known whitening ingredients that are sufficiently satisfactory, and the development of better whitening agents is desired.

  Stem Cell Factor (SCF), also called Must Cell Growth Factor, C-Kit Ligand, Steel Factor, etc., is a protein produced from keratinocytes, fibroblasts, vascular endothelial cells, bone marrow stromal cells, etc. It is. SCF is known to have an action of promoting proliferation and differentiation of pluripotent hematopoietic stem cells, germ cells, mast cells, megakaryocyte progenitor cells, granulocyte / macrophage progenitor cells, pigment cells and the like. Moreover, it is known that the expression of SCF is enhanced by a spot site, ultraviolet irradiation or the like (see Non-Patent Document 1).

  As SCF, a membrane-bound SCF composed of 273 amino acid residues and a secreted SCF that is cleaved by the action of a proteolytic enzyme and released from the membrane are known. Membrane-bound SCF binds to the SCF receptor of the pigment cell while bound to keratinocytes and promotes the proliferation of the pigment cell. Secreted SCF is cleaved at its binding site, released from the cell membrane, and promotes proliferation of pigment cells by binding to SCF receptors of pigment cells.

  In addition, SCF is a myeloblast in the presence of interleukin-3 (IL-3) and granulocyte macrophage colony stimulating factor (GM-CSF) in patients with acute myeloid leukemia. It is known to promote the growth of selenium (see Non-Patent Document 2).

  Therefore, abnormal production of SCF is thought to lead to abnormal growth of pigment cells, increase melanin production, and cause stains, buckwheat, dullness, and the like. Abnormal production of SCF is thought to lead to abnormal growth of myeloblasts, thereby causing diseases such as myelodysplastic syndrome and acute myeloid leukemia (AML).

  Therefore, suppressing the increase in the expression of SCF mRNA suppresses pigment cell proliferation, suppresses excessive production of melanin in the skin, and is considered useful for preventing or improving pigmentation, spots, buckwheat, etc. after sunburn. It is done. Further, suppressing the increase in the expression of SCF mRNA suppresses abnormal growth of myeloblasts, and is considered useful for the prevention or treatment of myelodysplastic syndrome, acute myeloid leukemia and the like.

Hachiya A et al., J. Invest. Dermatol., No. 116, 2001, pp. 578-586 Virginia C. Broudy et al., Blood, Vol.80, No.1, 1992, pp.60-67

  The object of the present invention is to find an SCF mRNA expression increase inhibitory action from among highly safe natural products, and to provide an SCF expression increase inhibitor that uses it as an active ingredient.

  In order to solve the above-mentioned problems, the SCF mRNA expression increase inhibitor of the present invention is one or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract. Is contained as an active ingredient.

  In addition, the preventive / therapeutic agent for diseases caused by increased expression of SCF mRNA of the present invention is one or more plants selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract. It contains an extract as an active ingredient.

  According to the present invention, it contains one or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract as an active ingredient, and is excellent in safety. It is possible to provide an SCF mRNA expression increase inhibitor and a prophylactic / therapeutic agent for a disease caused by an increase in SCF mRNA expression.

Hereinafter, the present invention will be described in detail.
[Suppressor of elevated stem cell growth factor expression]
The SCF mRNA expression increase inhibitor of the present invention contains, as an active ingredient, one or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract.

  Here, in the present invention, “extract” refers to an extract obtained from the above plant as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product thereof. Both purified products and purified products are included.

  Extract raw materials used in the present invention are horsetail, Dutch pepper, chamomile and assembly. When using two or more kinds of plants as an extraction raw material, the above plants can be used in any combination.

  Horsetail (scientific name: Equisetum avens) is a plant belonging to the family Horsetail, and is widely distributed in the warm zone of the northern hemisphere, and can be easily obtained from these regions. As a site | part which can be used as an extraction raw material, a underground part, an above-ground part, or the mixture of these parts etc. are mentioned, for example, Preferably it is an above-ground part.

  Dutch pepper (scientific name: Nasturium officinale) is a plant belonging to the Brassicaceae family, is native to Europe from Central Asia, and is also widely grown in Japan, and can be easily obtained from these regions. Examples of the part that can be used as the raw material for extraction include leaves, stems, flowers, roots, above-ground parts, or a mixture of these parts, with the leaves and above-ground parts being preferred.

  Chamomile (scientific name: Matricaria chamomilla) is an annual plant belonging to the family Asteraceae and is native to Europe and West Asia and can be easily obtained from these regions. Examples of a part that can be used as an extraction raw material include a leaf part, a stem part, an above-ground part, a flower part, a fruit part, a seed part, a root part, a whole plant, or a mixture of these parts. is there.

  The assembly (scientific name: Swartia japonica) is a plant belonging to the Gentianaceae family and grows naturally in the mountains from Kyushu to Hokkaido and can be easily obtained from these regions. Examples of the part that can be used as the extraction raw material include a leaf part, a trunk part, an above-ground part, an underground part, a whole grass, or a mixture of these parts, and the whole is preferable.

  Details of the substance having an inhibitory effect on the increase in SCF mRNA expression contained in the extract of Japanese horsetail, Dutch pepper extract, chamomile extract, or assembly extract are unknown. An extract having an inhibitory effect on the increase in SCF mRNA expression can be obtained from Dutch pepper, chamomile or assembly.

  For example, after drying the plant, it is pulverized as it is or using a crusher, and subjected to extraction with an extraction solvent, whereby an extract having an inhibitory effect on SCF mRNA expression increase can be obtained. Drying may be performed in the sun or may be performed using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing pretreatment such as degreasing, extraction treatment with the polar solvent of the plant can be efficiently performed.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These may be used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. Is preferred.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a liquid mixture of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by weight of a lower aliphatic alcohol with respect to 10 parts by weight of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by mass of a lower aliphatic ketone with 10 parts by mass of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 10 to 90 parts by mass of polyhydric alcohol with respect to parts by mass.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in 5 to 15 times the extraction solvent (mass ratio) of the extraction raw material, the soluble components are eluted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof. Processing may be performed.

  Purification can be performed by, for example, activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like. The obtained extract can be used as it is as an active ingredient of an SCF mRNA expression increase inhibitor, but a concentrate or a dried product is easier to use.

  The extract of Japanese horsetail, Dutch pepper, chamomile extract or assembly extract has a specific odor and taste, so it is purified for the purpose of decolorization, deodorization, etc. within a range that does not cause a decrease in its physiological activity. However, since it is not used in a large amount when added to an SCF mRNA expression increase inhibitor, there is no practical problem even if it is not purified.

  Since the horsetail extract, Dutch pepper extract, chamomile extract or assembly extract obtained as described above has an SCF mRNA expression increase inhibitory action, the action is effective for an SCF mRNA expression increase inhibitor. It can be used as a component.

  In addition, horse chestnut extract, Dutch pepper extract, chamomile extract or assembly extract uses its SCF mRNA expression increase suppressive action to prevent or treat diseases caused by increased SCF production, specifically bone marrow. It can also be used as an active ingredient such as an agent for preventing / treating dysplasia syndrome, an agent for preventing / treating acute myeloid leukemia, and an antitumor agent.

  The SCF mRNA expression increase inhibitor of the present invention may consist of only one or two or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract, One or two or more kinds of plant extracts selected from the group consisting of Japanese horsetail extract, Dutch pepper extract, chamomile extract and assembly extract may be formulated.

  One or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract are a pharmaceutically acceptable carrier such as dextrin, cyclodextrin, etc. Using an auxiliary agent, it can be provided in the form of an arbitrary dosage form such as powder, granule, tablet or liquid according to a conventional method. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring agent and the like can be used. One or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract can be used in combination with other compositions. It can be used as a solution for external use, a patch and the like.

  In addition, the SCF mRNA expression increase inhibitor of the present invention includes, as necessary, other natural extracts having an SCF mRNA expression increase suppression action, a group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract. It can mix | blend with the 1 type, or 2 or more types of plant extract chosen from more, and can use it as an active ingredient.

  Examples of the method for administering the SCF mRNA expression increase inhibitor of the present invention to a patient include subcutaneous tissue administration, intramuscular administration, intravenous administration, oral administration, transdermal administration, and the like. -A method suitable for treatment or the like may be appropriately selected. In addition, the dose of the SCF mRNA expression increase inhibitor of the present invention may be appropriately increased or decreased depending on the type of disease, severity, individual differences among patients, administration method, administration period, and the like.

  The SCF mRNA expression increase inhibitor of the present invention has the effect of suppressing the increase in SCF mRNA expression possessed by one or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract. Can suppress the production of SCF, thereby suppressing the proliferation of pigment cells and the production of melanin, and can prevent or ameliorate spots, buckwheat, skin pigmentation, etc., and can obtain a whitening effect .

  In addition, abnormal growth of myeloblasts can be prevented through the SCF mRNA expression increase inhibitory action of one or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract. It is possible to suppress, thereby preventing, treating or improving diseases such as myelodysplastic syndrome and acute myeloid leukemia.

  However, the SCF mRNA expression increase inhibitor of the present invention can be used for all purposes that are meaningful for exhibiting the SCF mRNA expression increase suppression action in addition to these applications.

[Skin cosmetic]
An inhibitor of SCF mRNA expression increase comprising, as an active ingredient, one or more plant extracts selected from the group consisting of a horsetail extract, Dutch pepper extract, chamomile extract and assembly extract of the present invention, It can be used by blending with the material.

  The amount of SCF mRNA expression increase inhibitor in skin cosmetics can be adjusted as appropriate depending on the type of skin cosmetics, physiological activity of the extract, etc., but the preferred compounding rate is 0 in terms of a standard extract. 0.001 to 10% by mass.

  Skin cosmetics to which the SCF mRNA expression increase inhibitor of the present invention can be blended are various main agents that are usually used in the production of skin cosmetics as needed, as long as the effects of the SCF mRNA expression increase inhibitor of the present invention are not impaired. And auxiliary agents can be used in combination.

  Examples of components that can be used in combination with the SCF mRNA expression increase inhibitor of the present invention as a skin cosmetic constituent include humectants such as glycerin, collagen, hyaluronic acid and salts thereof, chondroitinic acid and salts thereof, chitin and chitosan; UV absorbers such as amyl aminobenzoate; complex lipids such as glycerophospholipid and sphingophospholipid; β-carotene, oil-soluble licorice extract, lycochalcone A, baicalin, baicalein, and other substances having an active oxygen scavenging action; azulene, glycyrrhizin Acids and salts thereof, glycyrrhetinic acid and derivatives thereof, anti-inflammatory substances such as zinc oxide; vitamins and derivatives thereof such as riboflavin, tropherol, ascorbic acid and folic acid; jojoba oil, lanolin, liquid paraffin, squalane, isostearyl alcohol Oily ingredients such as sodium stearyl sulfate, cetyl sulfate diethanolamine, surfactants such as glyceryl stearate; antioxidants such as sodium erythorbate; preservatives such as ethyl paraben; plant sterols; lipoproteins; bifidobacteria cultures Microbe-derived components such as lactic acid bacteria culture, yeast extract, and bucurium extract; algal extracts such as brown algae extract and red algae extract; blood circulation promoters such as γ-oryzanol; antiseborrheic agents such as sulfur; Alcohol; thickeners such as carboxy polymer; titanium yellow, safflower, and other colorants. When used in this way, it becomes a more general product, and thereby a synergistic effect with other active ingredients used in combination may lead to a superior effect than would normally be expected.

  The above skin cosmetics suppress the growth of pigment cells and the production of melanin through the SCF mRNA expression increase inhibitory action possessed by the SCF mRNA expression increase inhibitor as a constituent component thereof, thereby preventing or improving spots, freckles, skin pigmentation, etc. And a whitening effect can be obtained.

  The SCF mRNA expression increase inhibitor of the present invention is suitably applied to humans, but can also be applied to animals other than humans as long as each effect is exhibited.

  Hereinafter, although a manufacture example, a test example, and a compounding example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

  In addition, a horsetail extract, Dutch pepper extract, chamomile extract and assembly extract used in this example are a horsetail extract, Dutch pepper extract, chamomile extract and assembly extract (all manufactured by Maruzen Pharmaceutical Co., Ltd.). Each lyophilized product was used as an extract (sample) of each plant.

[Test Example 1] SCF mRNA expression increase inhibitory action test Each plant extract was tested for SCF mRNA expression increase suppressive action as follows.

(1) Preparation of single-stranded DNA Normal neonatal epidermal keratinocytes (NHEK) were seeded in a 35 mm dish, and cultured under conditions of 37 ° C., 5% CO 2 -95% air for 24 hours. After completion of the culture, it was washed with 1 mL of a phosphate physiological buffer and replaced with 1 mL of Hanks' solution. The ultraviolet ray UV-B was irradiated at 50 mJ / cm 2 using an ultraviolet ray irradiation device equipped with four TOREX FL20SE-30 / DMRs as a light source. Immediately after the irradiation, the medium was replaced with a medium supplemented with a sample (samples 1 to 11) having a predetermined concentration (see Table 1) and cultured for 24 hours. After completion of the culture, total RNA was prepared by a conventional method. In addition, total RNA was similarly prepared for cells cultured with “no sample added / no UV irradiation” and “no sample added / UV irradiation”. Total RNA was prepared using the following method.

  Dissolve the cells in 1 mL of RNA extraction reagent (product name: ISOGEN, Nippon Gene), add 200 μL of chloroform, isolate the upper RNA layer by centrifugation (12,000 rpm, 4 ° C., 15 minutes), and further isopropanol. Concentrated with. Concentrated and precipitated total RNA was dissolved in TE solution (10 mM Tris-HCl / 1 mM EDTA, pH = 8.0) to prepare a total RNA preparation, and a PCR device (product name: TaKaRa PCR Themal Cycler MP, manufactured by Takara Bio Inc.) ) And a real-time PCR-dedicated reverse transcription kit (product name: TaKaRa ExScript RT reagent Kit, manufactured by Takara Bio Inc.), single-stranded DNA used as a template for measuring the expression level of SCF mRNA was synthesized.

(2) Real-time-PCR reaction using the cyber green method A sense primer and an antisense primer having the following sequences were prepared as primers for SCF gene amplification (manufactured by Takara Bio Inc.).
Sense primer: 5'-cccttaggaatgacagcagtagca-3 '
Antisense primer: 5'-gcccttgtaagacttggctgtctc-3 '

In addition, a sense primer and an antisense primer having the following sequences were prepared as primers for amplifying the G3PDH gene as an internal standard (manufactured by Takara Bio Inc.).
Sense primer: 5'-gcaccgtcaaggctgagaac-3 '
Antisense primer: 5'-atggtggtgaagacgccagt-3 '

  Single-stranded DNA prepared from total RNA preparations prepared from cells cultured in “No sample added / No UV irradiation”, “No sample added / With UV irradiation” and “Sample added / With UV irradiation”, respectively. Real-time PCR device (Product name: Real Time PCR System Smart Cycler II, manufactured by Cepheid) and Real-time PCR kit (Product name: SYBR Premix Ex Taq, manufactured by Takara Bio Inc.) A real-time PCR reaction was performed. In the single-stranded DNA solution for preparing a calibration curve, the relative value of the stock solution is set to “100,000” for convenience, and the concentration values “100,000”, “10000”, “1000”, “100” are repeatedly diluted 10 times thereafter. ”And“ 10 ”. The reaction was held at 95 ° C. for 10 seconds, followed by 45 cycles of 95 ° C. for 5 seconds and 57 ° C. for 20 seconds, and the amount of luminescence of the cyber green dye was measured for each cycle.

(3) Analysis An amplification curve of a DNA fragment encoding each of SCF and G3PDH was prepared from the amount of luminescence of the cyber green dye in each cycle. A calibration curve was prepared from the amplification curve of a dilution series of a single-stranded DNA solution for preparing a calibration curve, with the horizontal axis representing the concentration and the vertical axis representing the number of cycles that maximized the second derivative of the amplification curve. For each expression quantification sample, the number of cycles that maximized the second derivative of the amplification curve was plotted on a calibration curve, and the relative expression level was calculated. After correcting the expression level of SCF with the value of the expression level of G3PDH in the same sample, when the correction value of “no sample added / no UV irradiation” is set to 100, “no sample added / UV irradiation” and “ It was calculated as a correction value for “sample addition / with UV irradiation”.

The suppression rate (%) of SCF mRNA expression increase was calculated by the following formula.
SCF mRNA expression increase suppression rate (%) = {(A−B) − (A−C)} / (A−B) × 100
In the formula, A represents “correction value without sample addition / ultraviolet irradiation”, B represents “correction value without sample addition / ultraviolet irradiation”, and C represents “correction value with sample addition / ultraviolet irradiation”. Value ". The results of the SCF mRNA expression increase inhibitory effect test are shown in Table 1.

[Table 1]
Sample name Sample concentration (μg / mL) SCF mRNA expression increase suppression rate (%)
Horsetail extract 1.25 27.4
Dutch pepper extract 1.00 19.1
10.00 7.5
Chamomile extract 1.00 60.7
10.00 94.5
Assembly extract 1.00 100.0
10.00 100.0

  As shown in Table 1, it was confirmed that the horsetail extract, the Dutch pepper extract, the chamomile extract and the assembly extract have an excellent inhibitory effect on the increase in SCF mRNA expression.

[Formulation Example 1]
An emulsion having the following composition was produced by a conventional method.
Horsetail extract 0.01g
Jojoba oil 4.0g
Placenta extract 0.1g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
2.5 g of polyoxyethylene cetyl ether (20E.0)
Oleic acid polyoxyethylene sorbitan (20E.0) 2.0 g
Stearyl glycyrrhetinate 0.1g
1,3-butylene glycol 3.0 g
Hinokitiol 0.15g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A lotion having the following composition was produced by a conventional method.
Dutch pepper extract 2g
Glycerin 3g
1,3-butylene glycol 3g
Oleic acid polyoxyethylene sorbitan (20E.0) 0.5g
Methyl paraoxybenzoate 0.15g
Citric acid 0.1g
Sodium citrate 0.1g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A cream having the following composition was produced by a conventional method.
Chamomile extract 0.05g
Aloe extract 0.1g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Squalane 10.0g
Cetanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.0) 1.5g
3.0 g glyceryl monostearate
Oil soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Formulation Example 4]
A pack having the following composition was produced by a conventional method.
Assembly extract 0.05g
Yokuinin extract 0.1g
Polyvinyl alcohol 15g
Polyethylene glycol 3g
7g of propylene glycol
Ethanol 10g
0.05 g of methyl paraoxybenzoate
0.1g dipotassium glycyrrhizinate
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

  The inhibitor of stem cell growth factor (SCF) mRNA expression increase of the present invention is useful for preventing or improving skin pigmentation, sun spots, buckwheat and the like after sunburn. Moreover, the stem cell growth factor (SCF) mRNA expression increase inhibitor of the present invention is useful for prevention or treatment of myelodysplastic syndrome, acute myeloid leukemia and the like.

Claims (2)

  Stem cell growth factor (SCF) mRNA characterized by containing, as an active ingredient, one or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract Expression increase inhibitor.   Stem cell growth factor (SCF) mRNA characterized by containing, as an active ingredient, one or more plant extracts selected from the group consisting of horsetail extract, Dutch pepper extract, chamomile extract and assembly extract A prophylactic / therapeutic agent for diseases caused by increased expression of