JP2010510771A5 - - Google Patents
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- JP2010510771A5 JP2010510771A5 JP2009537717A JP2009537717A JP2010510771A5 JP 2010510771 A5 JP2010510771 A5 JP 2010510771A5 JP 2009537717 A JP2009537717 A JP 2009537717A JP 2009537717 A JP2009537717 A JP 2009537717A JP 2010510771 A5 JP2010510771 A5 JP 2010510771A5
- Authority
- JP
- Japan
- Prior art keywords
- post
- fret
- fluorescent compound
- translational modification
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 32
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 27
- 102000004169 proteins and genes Human genes 0.000 claims 25
- 108090000623 proteins and genes Proteins 0.000 claims 25
- 239000000758 substrate Substances 0.000 claims 24
- 239000007850 fluorescent dye Substances 0.000 claims 22
- 230000004481 post-translational protein modification Effects 0.000 claims 21
- 210000004027 cell Anatomy 0.000 claims 19
- 230000008878 coupling Effects 0.000 claims 17
- 238000010168 coupling process Methods 0.000 claims 17
- 238000005859 coupling reaction Methods 0.000 claims 17
- 102000004190 Enzymes Human genes 0.000 claims 14
- 108090000790 Enzymes Proteins 0.000 claims 14
- 230000027455 binding Effects 0.000 claims 11
- 150000001875 compounds Chemical class 0.000 claims 11
- 239000007822 coupling agent Substances 0.000 claims 10
- 238000005259 measurement Methods 0.000 claims 10
- 206010010144 Completed suicide Diseases 0.000 claims 8
- 230000000694 effects Effects 0.000 claims 5
- 239000013604 expression vector Substances 0.000 claims 5
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 4
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 4
- 108020001507 fusion proteins Proteins 0.000 claims 4
- 102000037865 fusion proteins Human genes 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 239000003795 chemical substances by application Substances 0.000 claims 3
- 239000003112 inhibitor Substances 0.000 claims 3
- 150000007523 nucleic acids Chemical group 0.000 claims 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims 2
- 108010022394 Threonine synthase Proteins 0.000 claims 2
- 210000000170 cell membrane Anatomy 0.000 claims 2
- 239000013522 chelant Substances 0.000 claims 2
- 102000004419 dihydrofolate reductase Human genes 0.000 claims 2
- 229910052761 rare earth metal Inorganic materials 0.000 claims 2
- 150000002910 rare earth metals Chemical group 0.000 claims 2
- 239000012429 reaction media Substances 0.000 claims 2
- 230000000638 stimulation Effects 0.000 claims 2
- XWNJMSJGJFSGRY-UHFFFAOYSA-N 2-(benzylamino)-3,7-dihydropurin-6-one Chemical group N1C=2N=CNC=2C(=O)N=C1NCC1=CC=CC=C1 XWNJMSJGJFSGRY-UHFFFAOYSA-N 0.000 claims 1
- 230000005730 ADP ribosylation Effects 0.000 claims 1
- 108010047357 Luminescent Proteins Proteins 0.000 claims 1
- 102000006830 Luminescent Proteins Human genes 0.000 claims 1
- 108010076693 O(6)-Methylguanine-DNA Methyltransferase Proteins 0.000 claims 1
- 108090000848 Ubiquitin Proteins 0.000 claims 1
- 102000044159 Ubiquitin Human genes 0.000 claims 1
- 229930003427 Vitamin E Natural products 0.000 claims 1
- 230000021736 acetylation Effects 0.000 claims 1
- 238000006640 acetylation reaction Methods 0.000 claims 1
- 230000004913 activation Effects 0.000 claims 1
- 150000001348 alkyl chlorides Chemical class 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 108091092356 cellular DNA Proteins 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol group Chemical group [C@@H]1(CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C)[C@H](C)CCCC(C)C HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims 1
- 150000002148 esters Chemical class 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 239000010954 inorganic particle Substances 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 238000006011 modification reaction Methods 0.000 claims 1
- 108091005573 modified proteins Proteins 0.000 claims 1
- 102000035118 modified proteins Human genes 0.000 claims 1
- 238000006396 nitration reaction Methods 0.000 claims 1
- 108091008104 nucleic acid aptamers Proteins 0.000 claims 1
- 230000003204 osmotic effect Effects 0.000 claims 1
- 230000008823 permeabilization Effects 0.000 claims 1
- 230000000144 pharmacologic effect Effects 0.000 claims 1
- 230000026731 phosphorylation Effects 0.000 claims 1
- 238000006366 phosphorylation reaction Methods 0.000 claims 1
- 230000005731 poly ADP ribosylation Effects 0.000 claims 1
- 102000035123 post-translationally modified proteins Human genes 0.000 claims 1
- 108091005626 post-translationally modified proteins Proteins 0.000 claims 1
- 230000013823 prenylation Effects 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 230000017854 proteolysis Effects 0.000 claims 1
- 230000009467 reduction Effects 0.000 claims 1
- 230000009870 specific binding Effects 0.000 claims 1
- 230000003595 spectral effect Effects 0.000 claims 1
- 230000004936 stimulating effect Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 230000010741 sumoylation Effects 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 235000019165 vitamin E Nutrition 0.000 claims 1
- 229940046009 vitamin E Drugs 0.000 claims 1
- 239000011709 vitamin E Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86108506P | 2006-11-27 | 2006-11-27 | |
| PCT/IB2007/004341 WO2008065540A2 (en) | 2006-11-27 | 2007-11-27 | Method for the detection of post-translational modifications |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010510771A JP2010510771A (ja) | 2010-04-08 |
| JP2010510771A5 true JP2010510771A5 (enExample) | 2010-11-25 |
Family
ID=39468330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009537717A Pending JP2010510771A (ja) | 2006-11-27 | 2007-11-27 | 翻訳後修飾の検出のための方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US8663928B2 (enExample) |
| EP (1) | EP2087355B1 (enExample) |
| JP (1) | JP2010510771A (enExample) |
| WO (1) | WO2008065540A2 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011090159A1 (ja) * | 2010-01-22 | 2011-07-28 | 独立行政法人科学技術振興機構 | 蛋白質の分解活性を測定するためのプローブ試薬 |
| CN103630691B (zh) * | 2012-08-24 | 2015-07-08 | 中国科学院深圳先进技术研究院 | 小泛素样修饰的生物检测方法 |
| WO2014108480A1 (en) * | 2013-01-09 | 2014-07-17 | Friedrich-Alexander-Universitaet Erlangen-Nuernberg | Method for in vitro detection and monitoring of a disease by measuring disease-associated protease activity in extracellular vesicles |
| CN104749143A (zh) * | 2013-12-31 | 2015-07-01 | 深圳先进技术研究院 | 一种蛋白质类泛素化修饰的检测方法及其应用 |
| KR101750407B1 (ko) | 2014-11-10 | 2017-06-27 | 한양대학교 산학협력단 | 프로틴 카이네이즈 a 기질 인산화펩타이드에 결합하는 알엔에이 앱타머 |
| CN107463795A (zh) * | 2017-08-02 | 2017-12-12 | 南昌大学 | 一种识别酪氨酸翻译后修饰位点的预测算法 |
| US11604186B2 (en) * | 2018-10-17 | 2023-03-14 | Molecular Devices (Austria) GmbH | Real time western blot assays utilizing fluorescence resonance energy transfer (FRET) |
| WO2023091395A1 (en) * | 2021-11-16 | 2023-05-25 | Biogen Ma Inc. | Method for detecting a lipidated protein via fluorescence resonance energy transfer (fret) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6900304B2 (en) | 1996-01-31 | 2005-05-31 | The Regents Of The University Of California | Emission ratiometric indicators of phosphorylation |
| GB9901072D0 (en) * | 1999-01-19 | 1999-03-10 | Imp Cancer Res Tech | Methods for detecting changes to a macromolecular component of a cell |
| GB9927331D0 (en) | 1999-11-18 | 2000-01-12 | Fluorescience Ltd | Assay for measuring enzyme activity in vivo |
| JP2004537047A (ja) * | 2001-06-14 | 2004-12-09 | アナディーズ ファーマスーティカルズ，アイエヌシー． | 標的分子のリガンドをスクリーニングする方法 |
| CA2445420A1 (en) * | 2003-07-29 | 2005-01-29 | Invitrogen Corporation | Kinase and phosphatase assays |
| CA2584131A1 (en) * | 2004-10-15 | 2006-04-27 | Signal Pharmaceuticals, Llc | P27 ubiquitination assay and methods of use |
-
2007
- 2007-11-27 EP EP07859356.3A patent/EP2087355B1/en active Active
- 2007-11-27 US US12/516,333 patent/US8663928B2/en active Active
- 2007-11-27 JP JP2009537717A patent/JP2010510771A/ja active Pending
- 2007-11-27 WO PCT/IB2007/004341 patent/WO2008065540A2/en not_active Ceased
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