JP2010248085A - Fungicide - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a natural substance-based fungicide which is friendly for the environment and human bodies and is high in safety. <P>SOLUTION: The fungicide includes the extract from the material, Tabebuia spp., as an active ingredient. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a fungicide.

  Antifungal agents based on silver-substituted inorganic ion exchangers are known as antifungal agents used in the final product leather, but most leather products have not been antifungal treated. At the import, transportation and storage stages of leather products, and also at various stages after sales, mainly molds derived from the environment adhere to the surface of leather products, which germinate due to conditions such as humidity and temperature, and produce mold. There was a problem of causing deterioration in quality and function.

  On the other hand, an antifungal agent for leather is known as a chemical agent used for antifungal treatment of raw leather. This fungicide for leather is intended to suppress the occurrence of mold during the raw leather manufacturing process such as chrome tanning bath, tannin tanning bath, greasing bath, water bath, pickling bath, etc. Excellent antifungal performance at. However, at the time of shipment as leather for product raw materials, the fungicidal component is hardly left in the leather for product raw materials due to washing treatment and drying treatment during the manufacturing process, so the product after shipment has sufficient fungicidal performance. Not granted. The fungicides for leather are generally trichlorphosphate, parachlormetacresol, organotin, benzimidazole, benzimidazole organic nitrogen halogen, organic sulfur, chlorinated aliphatic, Organic iodine-based and imazalyl sulfate-based antifungal agents are known, but in recent years, considering the effects on the environment and the human body, natural products that are considered to be safer and safer for the environment and the human body There is a need for agents.

  By the way, as an example using a natural product, there is an antibacterial agent of Patent Document 1, and it has been reported that an acetone extract of Tabebuia spp. Material has an antibacterial action against bacteria. However, the antibacterial agent of Patent Document 1 is intended for Gram-positive bacteria and negative bacteria, and the structure and life cycle of fungi (fungi (filamentous fungi)) targeted by the present invention are completely different. Since they are different microorganisms, Patent Document 1 does not suggest the effectiveness of preventing the occurrence of fungi (fungi (filamentous fungi)).

JP 2006-166803 A

  The present invention has been made in view of the circumstances as described above, and provides an antifungal agent of a natural product type that is applicable to leather or leather products and is gentler on the environment and human body and higher in safety. Is an issue.

  The present invention is characterized by the following.

  First, the fungicides of the present invention are characterized by containing an extract of Tabebuia spp. As an active ingredient.

  Second, the extract is an alcohol extract.

  Third, the leather or leather product of the present invention is coated or smeared with the antifungal agent of the first or second invention.

  According to the present invention, by applying or smearing on leather or leather products, germination of environmentally derived mold spores adhering to the surfaces of leather and leather products can be suppressed, and generation of mold can be prevented or reduced. In addition, unlike conventional antifungal agents containing heavy metals and organochlorine compounds that may adversely affect the environment and the human body, the present invention uses natural products as active ingredients. An antifungal agent having a small impact and high safety is provided.

  The fungicides of the present invention can be applied to various industrial materials and products, including plastic products, paint products, adhesives, paper products such as tissue paper and wallpaper, in addition to leather and leather products. Can be granted.

It is the schematic of the manufacturing process of the antifungal agent of this invention. It is a gas chromatograph mass spectrometry result of the methanol extract of ipe material.

  The fungicide of the present invention contains an extract of Tabebuia spp. Material as an active ingredient.

  The ipe material used in the present invention is a broad-leaved wood of the Nounzenkazura family widely distributed in the Amazon River basin of the South American continent such as Brazil and Peru. Although it is imported into Japan as an exterior material, it is difficult to cut during lumbering, so the lumber yield is lower than the lumber yield of Japanese conifers and hardwoods (about 60%). A large amount of unused waste such as Since the end material of ipe material has high air-dry density and is difficult to reuse for particle board, at present, only a part of it is used as fuel without being used, but in the present invention this ipe material waste Can be used, and it is also expected to be an effective utilization method for waste of ipe materials.

  The antifungal agent of the present invention is obtained, for example, through the manufacturing process shown in FIG. First, in order to increase the extraction efficiency, an ipe material such as an ipe end material is pulverized to an appropriate size into small pieces. In the examples described later, the sheet is cut into a size of about 20 mm (radial direction) × 20 mm (tangential direction) × 5 mm (fiber direction), but is not limited thereto. When using ipe lumber sawdust, pulverization is not necessary and it can be used in powder form. As the extraction solvent, an organic solvent generally used for wood, plants and the like can be used, and an alcohol solvent is particularly preferable. For example, lower alcohols such as methanol, ethanol, n-propyl alcohol, isobutyl or butyl alcohol can be exemplified as preferable examples. The extraction can be performed by heating reflux such as Soxhlet extraction, but may be immersion extraction. The extract (Ipe material extract component liquid) can be used as the fungicide of the present invention as it is, but it is concentrated or dried (Ipe material extract component dry product) to obtain a powder. In addition, it may be dissolved in an appropriate solvent to form a liquid, or a paste prepared by mixing and dispersing in a hydrophobic base (oil base) may be used as a fungicide. The concentration treatment can be performed, for example, by heating at 40 to 120 ° C. under reduced pressure or normal pressure for about 5 minutes to 3 hours. The drying treatment can also be performed by heating at 40 to 150 ° C. under reduced pressure or normal pressure, for example. In addition, the process conditions of a concentration process or a dry-solid process are not limited to this.

  The fungicides obtained as described above are, as shown in the examples described later, such as black mold (Aspergillus niger) and blue mold (Penicillium citrinum), which are known as molds that easily grow on leather. It is effective and can be expected to be effective against other Aspergillus genus and Penicillium genus having the same genus. By applying or smearing the antifungal agent to the surface of leather or leather goods, it is possible to impart excellent antifungal properties to the leather or leather goods. In particular, when applied to leather, the anti-fungal property is not lost by washing or drying during the subsequent manufacturing process from leather to leather products. Excellent effects such as shipping. Of course, not only leather and leather products, but also applicable to various industrial materials and products, including plastic products, paint products, adhesives, paper products such as tissue paper and wallpaper, leather and leather products Excellent antifungal properties can be imparted similarly to the above.

  While the present invention has been described based on the embodiments, the present invention is not limited to the above-described embodiments, and various modifications can be made without departing from the scope of the invention. Examples of the present invention will be specifically described below.

<Example 1>
Extracted solution obtained by extracting a small piece of ipe heartwood (about 20 mm (radial direction) x 20 mm (tangential direction) x 5 mm (fiber direction)) with a Soxhlet extractor for 8 hours and concentrating to dryness with an evaporator to dryness Got. Methanol was used as the extraction solvent. The yield (extract content) of the extract obtained was 11.7% when the extract was dried in a desiccator under reduced pressure and measured for mass. The obtained extract was ground into a powder form in a mortar to obtain a powder preparation.

The powdery preparation was dissolved in acetone, and the solution was applied to a leather piece (2 × 2 cm) to obtain a test piece. The test pieces are those having an application concentration of the ipe material extract component of 0.0 (control test), 0.5, 1.0, 2.0, 3.0, 4.0, 5.0 mg / cm ² , respectively. Got ready. The leather pieces used were raw leather for pig products that had been tanned.

  The performance as an antifungal agent of the ipe material extract component was evaluated as follows with reference to the paper disk method used for the antifungal efficacy test and the like.

  About 20 mL of potato dextrose agar medium (PDA medium) sterilized under high pressure steam was injected into a sterile petri dish having an inner diameter of 90 mm and solidified. JIS Z 2911: 2006 Fungi resistance test, Aspergillus niger van Tieghem NBRC6341 spore (5 platinum ears) specified in the test of leather and leather products, wetting agent-added sterilized water (sulfo-amber) Suspended in 10 ml of dioctyl sodium acid 50 mg / l) to form a single spore suspension. The single spore suspension was filtered through a funnel filled with sterilized glass wool, and humectant-added sterilized water was added to a total volume of 50 ml. The sterilized Conrage rod was immersed in the filtered single spore suspension, and the single spore suspension was immediately smeared on the potato dextrose agar medium using the Conrage rod. The prepared specimens are placed there, respectively, cultured for 7 days at a temperature of 28% and a relative humidity of 95% or more, and the occurrence of mold on the specimen surface is observed visually and using a stereomicroscope at a magnification of about 50 times to prevent mold. The performance (mold resistance) of the agent was evaluated. In addition, the test was implemented about the test piece which air-dried for 24 hours after apply | coating, and the test piece which air-dried for 48 hours after further leaching operation (stirring process in ion-exchange water for 1 hour).

  The evaluation criteria for the performance (mold resistance) of the fungicide is “2” when the occurrence of mold is found to be 1/3 or more of the area of the test piece, and the occurrence of mold is 1/3 of the area of the test piece. The case of less than 1 was evaluated as “1”, and the case of no occurrence of mold was evaluated as “0”. If the evaluation result is “1” or “0”, it can be determined that there is an antifungal effect.

  The results are shown in Table 1.

<Example 2>
In Example 1, instead of spores of Aspergillus niger van Tieghem NBRC6341, JIS Z 2911: 2006 fungus resistance test, Penicillium citrinum B The performance (mold resistance) of the fungicide was evaluated in the same manner as in Example 1 except that it was used.

  The results are shown in Table 2.

  The evaluations in parentheses in Tables 1 and 2 are the results of observation at about 50 times using a stereomicroscope, and are stricter observation conditions than the JIS standard that is visually confirmed and judged. In Tables 1 and 2, those that were evaluated as “traces” were those in which the occurrence of mold was slightly confirmed by observation with a stereomicroscope (about 50 times). The same visual observation result (JIS standard) is evaluated as “0”, and it is evaluated that there is sufficient antifungal performance.

From the above results, when the alcohol extract component of ipe material is applied to leather pieces at a concentration of 2.0 mg / cm ² or more, it exhibits a sufficient fungus growth-inhibiting action against Aspergillus niger van Tieghem NBRC6341. Was confirmed. For blue mold (Penicillium citrinum Thom NBRC6352), it was confirmed that when the alcohol extract component of the ipe material was applied to the leather pieces at a concentration of 1.0 mg / cm ² or more, a sufficient fungus growth inhibitory effect was shown. In addition, when the fungicidal agent of the present invention is used for the antifungal treatment of the leather raw leather because the effect of the fungus growth inhibiting action does not change even if the leaching operation (stirring treatment for 1 hour in ion-exchanged water) is performed after coating. It is possible to impart sufficient antifungal performance to leather products during the raw leather manufacturing process and after shipment.
<Example 3>
About the methanol extract of the ipe material obtained in Example 1, gas chromatograph mass spectrometry (GC part: JMS-GCmateII manufactured by JEOL Ltd., MS part: 6890A manufactured by Agilent, column HP-1φ0.32 mm × 30 m, film thickness 0.25 μm) was used to qualify the main components. The sample inlet temperature was 300 ° C., the detection part temperature was 300 ° C., the column initial temperature was 150 ° C., the column heating temperature was 10 ° C./min, and the column final temperature was 250 ° C. FIG. 2 shows the results of gas chromatograph mass spectrometry of the methanol extract of ipe material.

Claims (3)

  An antifungal agent comprising an extract of Tabebuia spp. As an active ingredient.   The antifungal agent according to claim 1, wherein the extract is an alcohol extract.   A leather or leather product, wherein the antifungal agent according to claim 1 or 2 is applied or smeared on a surface.