JP2010194042A - Structure for living body - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a structure for a living body having high strength such as an artificial bone, a bone prosthetic material, and a cell-culturing carrier and the like, capable of fixing and supporting cells and invading cells from various directions, obtaining enough efficiency for fixation, increase, and culture of cells, and promoting the growth. <P>SOLUTION: A structure for a living body configured with an aggregate of the fine forms is obtained by kneading and forming at least a main material and a binding material to produce a raw material of a columnar fine form having at least one penetrating hole in the longitudinal direction, and collecting by the raw materials at random to the three-dimensional direction and interconnected, and then by drying or calcining. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a biological structure composed of a micro-molded product having a through-hole, and more specifically, used for a biological tissue filling material such as a bone filling material, a cell culture carrier, etc., to fix and carry cells that become bones or blood vessels. It is related with the structure for biological bodies suitable for making it propagate, culture | cultivate and culture | cultivate.

  Conventionally, it has been studied to use a molded body made of metal, ceramic or the like as a biological tissue filling material or a cell culture base for bone loss or cell culture due to bone tumor extraction or trauma. As this molded body, for example, a porous body having a large number of pores for allowing cells such as osteoblasts to enter, settle, proliferate or culture is known. However, since it is difficult to control the size and distribution of pores in the production of a normal porous body, there are many cases where cells do not enter smoothly, and there is a problem that growth is not sufficiently promoted.

  For this reason, various studies have been made such as foam-like ones and honeycomb-like ones having many through holes in a certain direction. For example, a foam-shaped molded body is obtained by impregnating a resin foam with a ceramic slurry, degreasing a resin portion and then sintering, and is known as a three-dimensional network structure. Compared with the porous body, this molded body has preferable characteristics such as being able to enlarge the pores and setting the pore diameter to some extent and having multi-directionality. However, there is a problem that cracks are easily generated in the skeleton part due to production, and the strength becomes extremely low, and there is no one penetration part, so that the proliferation of cells cannot be sufficiently promoted. is there.

  As another example, a honeycomb-shaped one obtained by extrusion molding or the like can easily control the pores, and a single through-hole having a uniform diameter is obtained, so that sufficient cell growth is desired and the strength is stable. However, since all the through-holes are oriented in a certain direction, it becomes difficult to enter cells from other directions, and depending on the location, sufficient effects cannot be obtained for colonizing, supporting, growing and culturing cells. .

  For this reason, a method is known in which bead-shaped molded bodies having through-holes, that is, aggregates mainly composed of spherical shapes, are used together (see Patent Document 1). By using this method, it is possible to have a structure having a through-hole having a certain length and extending in multiple directions.

  However, in the above method, for example, when used as a bone grafting material, beads are gathered as they are in the defective part and embedded as they are, so that the molded body may move after implantation or jump during implantation. Management problems such as scattering occur, and even when beads are joined together, there is a problem that it is difficult to clearly obtain randomness in the direction of the through holes.

JP 2003-335574 A

  The present invention provides a high-strength biological structure capable of entering cells from multiple directions, obtaining sufficient effects of cell colonization, proliferation and culture, promoting growth, and having high strength. .

The present invention is a micro-molding manufactured through a series of processes in which a material of a columnar micro-molded product is produced, the materials are assembled randomly in a three-dimensional direction, the materials are joined together, and then dried or fired. The above-described problems have been solved by using a living body structure composed of a collection of objects, and the present invention has been completed. That is, the present invention
"1. At least the main material and the binder are kneaded and molded to produce a columnar micro-molded material having at least one through hole in the longitudinal direction, and the material is assembled randomly in the three-dimensional direction. A living body structure comprising an aggregate of micromolded articles obtained by bonding or joining and drying or firing.
2. The biological structure according to claim 1, wherein the joining of the materials is performed by activating the surfaces of the materials.
3. The biological structure according to claim 1, wherein the bonding between the materials is performed by applying an adhesive material to the surfaces of the materials.
4). A columnar micromolded material obtained by kneading at least a main material and a binder and forming a columnar micromolded material having at least one through hole in the longitudinal direction and firing the material. A biological structure comprising an assembly of micro-molded products obtained by applying a surface with an adhesive material, randomly assembling them in a three-dimensional direction, joining the micro-molded products, and drying or firing.
5). Items 1 to 4 are characterized in that the living body structure is formed by randomly assembling the materials in a three-dimensional direction so as to have three-dimensional network pores between the materials of the micro-molded product. The living body structure according to any one of the above.
6). 6. The biological structure according to any one of items 1 to 5, wherein the main material is calcium phosphate. ".

  According to the present invention, a columnar micro-molded material is prepared, and the material is randomly assembled in a three-dimensional direction, and the materials are joined to each other. The through-holes in the molded product have moderate linearity, and the direction of the holes is randomly oriented in any direction, facilitating the formation of blood vessels and cell proliferation, and at the same time easy cell entry from multiple directions It becomes. Therefore, it is possible to easily embed without worrying about the way and direction of embedding, and it is possible to arbitrarily change the diameter and length of the through hole of the micro-molded product and the size of the body structure. Therefore, it has excellent effects such as being able to be formed into a shape suitable for the type and amount of cells to be cultured or a place for supplementation.

It is a front view which shows an example of the biological body of this invention. It is a perspective view which shows an example of the micromolding used for this invention.

  In the present invention, at least a main material and a binder are kneaded, molded to produce a columnar micro-molded material having at least one through-hole in the longitudinal direction, and the materials are randomly assembled in a three-dimensional direction. This is achieved by, for example, forming a living body structure composed of an assembly of micro-molded products obtained by joining materials together and then drying or firing.

  As the main material of the present invention, any conventionally known material used for bone grafting materials, cell culture carriers and the like may be used, and examples thereof include metals, ceramics, resins, etc. Among these, ceramics such as aluminum oxide, zirconium oxide, and calcium phosphate are preferable, and in particular, a calcium phosphate-based ceramic is preferable because it has properties close to that of a biological material. Examples include calcium pyrophosphate, tricalcium phosphate, tetracalcium phosphate, octacalcium phosphate, hydroxyapatite, and Ca-deficient hydroxyapatite. These main materials can be used alone or in combination of two or more.

  Examples of the binder used in the present invention include resins, bioabsorbable polymers, carbon, and the like, and more specifically, polyvinyl alcohol, polyvinyl pyrrolidone, collagen, gelatin, and the like. These binders can be used alone or in combination of two or more.

  Other materials may be added in addition to the main material and the binder, and examples thereof include ceramics other than those used as the main material and the material for the binder, resins and materials suitably used for living bodies. .

  In the present invention, at least a main material and a binder are kneaded, molded to produce a columnar micro-molded material having at least one through-hole in the longitudinal direction, and the materials are randomly assembled in a three-dimensional direction. After the raw materials are joined together, a biological structure composed of an assembly of micro-molded products can be obtained by drying or firing.

  As a method of kneading and molding the main material and the binder, and forming a columnar micro-molded material having at least one through hole in the longitudinal direction, the binder is added to the main material, and water or Columnar micro-molded material having at least one through hole in the longitudinal direction after adding a solvent such as an organic solvent and kneading by a generally known method, followed by extrusion molding, injection molding, press molding, etc. Can be produced. At this time, by appropriately adjusting the outer diameter, the inner diameter, and the length, a target material for a minute molded article is produced.

  The method of joining the materials by randomly gathering the materials in a three-dimensional direction gathers at least a plurality of the materials and joins the materials by pressing or the like. At this time, it is preferable that the materials are joined by activating the surfaces of the materials. The surface of the material can be activated by applying a solvent such as water or an organic solvent. Examples of the solvent include ethers such as 1,4-dioxane, halogen-containing solvents such as methylene chloride and chloroform, esters such as ethyl acetate, alcohols such as methanol and ethanol, ketones such as acetone, and the like. A solvent, water, etc. are mentioned, It can use individually or in combination of 2 or more types. Optimally, it is water from the viewpoint of the working environment. Furthermore, for the purpose of improving the speed of drying and the like performed to obtain a biological structure composed of an assembly of micromolded products, water used for the activation treatment is combined with a water-soluble volatile organic solvent. It can be a mixed solvent. Examples of the water-soluble volatile organic solvent include alcohols such as methanol and ethanol, and ketones such as acetone.

  The surface of the material of the present invention is activated, which means that by applying a solvent, the binding material on the surface of the columnar micro-molded material becomes wet with the solvent, and a part of the binding material dissolves and swells. By causing such changes, the ability of the materials to gather and join is expressed. In order to activate the surface of the material, it is preferable to take a method of spraying a solvent on the surface of the material or immersing the material in a solvent from the viewpoint of easy production.

  Moreover, the joining of the raw materials of the columnar micro-molded product may be performed by applying an adhesive material to the surface of the raw material. The adhesive material can be applied to the surface of the material, for example, when the material is randomly assembled in a three-dimensional direction. As a coating method, it is preferable to take a method of spraying on the surface of the material or immersing the material in an adhesive material solution from the viewpoint of easy manufacture. In the case of using an adhesive material, examples of the adhesive material include natural resins, synthetic resins, collagen, gelatin, and the like, and these aqueous solutions may be used. When using an adhesive material, it is practically sufficient, but the joining of materials tends to be slightly inferior in randomness compared to the case where the surfaces of the materials are activated and joined, which is somewhat complicated in production. There is a tendency to become.

  The method of gathering the materials randomly in the three-dimensional direction and joining the materials together, followed by drying or firing, to obtain a biological structure as an aggregate of micro-molded products is assembling the materials randomly in the three-dimensional direction. After joining the materials together, a living body structure made of an aggregate of micro-molded products can be obtained by drying at room temperature or heating, or baking at a high temperature of 600 ° C. or higher.

  At this time, in the case of only drying, when the bonding between the materials is performed by activating the surface of the material, the bonding material in the material serves as a medium, and the bonding between the materials is bonded to the surface of the material. When the material is applied and bonded, the adhesive material itself serves as a medium, and a biological structure composed of an assembly of micro-molded products is obtained. In addition, in the case of firing, when the surfaces of the materials are activated to join the materials, the carbon obtained by firing the sintering force of the main material in the materials and / or the binder is fired. As a medium, a biological structure composed of an aggregate of micro-molded products can be obtained. In addition, when adhesive materials are applied to the surfaces of the materials for bonding, the carbon obtained from the pressure-sensitive adhesive material is mediated by firing, and a biological structure consisting of aggregates of micro-molded products is obtained. It is done.

  Further, the present invention is obtained by kneading at least a main material and a binder, forming a columnar micro-molded material having at least one through-hole in the longitudinal direction, and firing the material. An adhesive material is applied to the surface of the micromolded product, randomly assembled in the three-dimensional direction, and then dried or baked to obtain a biological structure composed of the aggregate of micromolded products. At this time, the method of applying the adhesive material to the surface of the micro-molded product can be the same method as the method of applying the adhesive material to the surface of the material and joining the materials. Further, an adhesive material is applied to the surface of the micro-molded product, and the micro-molded product is joined in a random manner in the three-dimensional direction, and then dried or fired to obtain a biological structure as an aggregate of the micro-molded product. As for the method, the materials can be obtained in the same manner as the method of obtaining the living body structure as an aggregate of micro-molded products by randomly assembling the materials in a three-dimensional direction and joining the materials together, followed by drying or firing. I can do it.

  Next, the biological structure of the present invention will be described with reference to the drawings. FIG. 1 is a front view showing a living body structure 1 of the present invention, and FIG. 2 is a perspective view showing a micro-molded product 2 used in the present invention. The micro-molded product 2 of the present invention has a columnar shape and has at least one through-hole 3 in the longitudinal direction, the direction of the through-hole 3 is randomly gathered in a three-dimensional direction, and is a living body composed of an assembly of micro-molded products 2. The structure 1 for a structure is comprised, and the three-dimensional network-like pore 4 is formed in the gap | interval between the micromolded products 2 in an assembly. The pores 4 are formed by randomly arranging the through-holes 3 in the three-dimensional direction. However, when the three-dimensional network-like pores 4 are provided, the biological structure of the present invention causes the formation of blood vessels and the proliferation of cells. At the same time, the effect of facilitating cell entry from multiple directions can be further increased.

  As the external shape of the micro-molded product 2 used in the present invention, a columnar shape is used, and examples thereof include a columnar shape, a triangular column shape, a quadrangular column shape, and a hexagonal column shape. These micro-molded products can be used in combination of one type of outer shape or two or more types of outer shapes. By using the columnar shape, it becomes easy to randomly arrange the micro-molded products in the three-dimensional direction, and as a result, the desired pores 4 are easily obtained in the gaps between the micro-molded products 2. That is, when the columnar micro-molded products are assembled at random, only a part of the surface is joined to each other, and most of the surface is in a three-dimensional intricate state without joining. This void portion presents three-dimensional network pores between the micro-molded products. The micro-molded product can be used in any shape as long as it is columnar, but in particular, it is cylindrical in that a three-dimensional network-like pore 4 having a clear shape is easily obtained. It is preferable.

  The L / D (L: length, D: outer diameter) of the micromolded product 2 in the biological structure of the present invention is arbitrary, but is preferably 1.5 or more, and particularly preferably 3 or more. . When L / D is less than 1.5, it becomes infinitely disk-shaped or spheroidized, so that it is easy to overlap closely even if the hole directions of the through holes are randomly positioned. There is a risk that it will be difficult to manufacture. As a result, it may be difficult to obtain pores in the gaps between the micro-molded products.

  The pore diameter of the through-hole 3 included in the micro-molded product 2 may be set as long as cells, blood vessels, bones, etc. are sufficiently generated and can be grown, and can be set as appropriate according to use conditions, for example, 10 to 1000 μm. A range of 200 to 1000 μm, which is a diameter suitable for cell entry, is preferable. Further, the number of through holes is at least one, and typically a pipe shape is preferable as shown in FIG. 2, but a honeycomb shape having a plurality of through holes can also be used, and one columnar shape Of these micro-molded products, those having through-holes having different hole diameters can be used. The cross-sectional shape of the through hole is arbitrary, such as a circle, an ellipse, a triangle, a quadrangle, and a hexagon, but a circular one is preferably used.

  The living body structure of the present invention maintains the structure only by joining the contact portions that are part of the surface of the columnar micromolded product. Since it is a collective shape that is randomly entangled with each other, it has a feature that it is difficult to be crushed and broken.

  In the present invention, when the adhesive material is applied and bonded to each other between the raw materials or the micro-molded products, the adhesive material applied to the surface of the micro-molded product or the carbon obtained from the adhesive material by firing, and the micro-molding In the case where the material contained in the object is the same material, the bonding force is improved, and it is particularly preferable that the material is difficult to break.

  Furthermore, when joining materials together by activating the surfaces of the materials, extra materials do not enter, it is easy to make, and there is less complexity in the process, so the degree of randomness is sufficiently improved. As a result, it is easy to obtain a state in which the gap between the micro-molded products 2 maintains a clear shape as it is, and there are various advantages such that the three-dimensional network-like pores 4 can be effectively used. This is preferable.

  Thus, according to the manufacturing process of the biological structure of the present invention, a columnar micromolded material is produced, the materials are assembled randomly in a three-dimensional direction, and the materials are joined together, and then dried. Alternatively, it is possible to obtain a living body structure composed of an assembly of micro-molded products by firing, and to obtain an excellent living body structure that solves the conventional problems.

  Examples of the present invention will be described below, but the present invention is not limited thereto.

Example 1
Tricalcium phosphate (main material) 80 parts by mass
Polyvinyl alcohol (binding material) 20 parts by mass
100 parts by mass of ion-exchanged water The above compound was kneaded and extruded to produce a large number of cylindrical micromolded materials having through-holes having a length of 2.0 mm, an outer diameter of 0.5 mm, and a pore diameter of 300 μm. This material was assembled at random in a cube with a side of 0.5 cm. After spraying water on this aggregate, wetting the binder in the material with water to activate the surface of the material, and then pressing it lightly, the aggregate is obtained with the materials joined together. It was. This aggregate was baked in an oxygen atmosphere at a maximum temperature of 1100 ° C. to obtain a biological structure composed of aggregates of micro-molded articles in which the direction of the through hole made of tricalcium phosphate was random in the three-dimensional direction. . In this biological structure, the sintering force of the main material serves as a medium, and cylindrical micro-molded products are joined to each other.
This living body structure is white and has a very good degree of randomness and is not easily destroyed. In addition, pores with a three-dimensional network structure are clearly formed in the gaps between micro-molded products. It was done. When this biological structure was embedded as a bone filler for rabbits and taken out after 4 weeks, osteoblasts proliferated in the through holes and in the pores of the gaps, and sufficient growth was observed.

Example 2
A large number of cylindrical micromolded materials were produced by the same method as in Example 1. This material was assembled at random in a cube with a side of 0.5 cm. By spraying a mixed solvent of water / ethanol (1/1 mass ratio) on this aggregate, wetting the binding material in the material with water to activate the surface of the material, and then pressing the material lightly, the material The aggregate was obtained with the two joined. This aggregate is baked in an argon atmosphere at a maximum temperature of 1100 ° C., and a biological structure composed of aggregates of micro-molded products in which the direction of the through holes made of tricalcium phosphate and carbon is random in a three-dimensional direction. Obtained. In this biological structure, carbon obtained from the binder was mediated by firing in an argon atmosphere, and cylindrical micro-molded products were joined to each other.
This biological structure had the same characteristics as Example 1 except that it was black.

Example 3
Tricalcium phosphate (main material) 80 parts by mass
Gelatin (binding material) 20 parts by mass
100 parts by mass of ion-exchanged water The above compound was kneaded and extruded to produce a large number of cylindrical micromolded materials having through-holes having a length of 2.0 mm, an outer diameter of 0.5 mm, and a pore diameter of 300 μm. This material was assembled at random in a cube with a side of 0.5 cm. By spraying a mixed solvent of water / ethanol (1/1 mass ratio) on this aggregate, wetting the binding material in the material with water to activate the surface of the material, and then pressing the material lightly, the material The aggregate was obtained with the two joined. This aggregate is dried at 100 ° C. in an air atmosphere to obtain a biological structure composed of aggregates of micro-molded articles in which the direction of the through hole made of tricalcium phosphate and a binder is random in a three-dimensional direction. It was. In this structure for living body, cylindrical micro-molded products were joined together by the adhesive force of the binding material.
This living body structure was white and had the same characteristics as in Example 1.

Example 4
A large number of cylindrical micromolded materials were produced by the same method as in Example 1. By spraying a 20% aqueous solution of polyvinyl alcohol (the same as the binder) on the material as an adhesive, the surface of the material was covered with the adhesive. The material whose surface was covered with an adhesive material was randomly assembled in a cube having a side of 0.5 cm. By pressing the assembled materials lightly, the aggregates were obtained with the materials joined together. This aggregate is baked in an argon atmosphere at a maximum temperature of 1100 ° C., and a biological structure composed of aggregates of micro-molded products in which the direction of the through holes made of tricalcium phosphate and carbon is random in a three-dimensional direction. Obtained. This living body structure was baked in an argon atmosphere, and the carbon obtained from the binder and the adhesive was used as a medium, and the cylindrical micro-molded products were joined together.
This living body structure was black and had a characteristic that the degree of randomness was slightly less than that of Example 1 and the number of three-dimensional network pores was slightly reduced, but it was difficult to break.

Example 5
A large number of cylindrical micromolded materials were produced by the same method as in Example 1. This material was fired in an oxygen atmosphere at a maximum temperature of 1100 ° C. to obtain a cylindrical micro-molded product. The surface of the micro-molded product was covered with an adhesive material by immersing this micro-molded product in a 20% aqueous solution of collagen as an adhesive material. The micro-molded product whose surface was covered with an adhesive material was randomly assembled into a cube having a side of 0.5 cm. By pressing this assembled material lightly, an aggregate was obtained in a state where the micro-molded products were joined together. This aggregate was dried at 100 ° C. in an air atmosphere to obtain a biological structure composed of aggregates of micro-molded articles in which the direction of the through hole made of tricalcium phosphate was random in the three-dimensional direction. In this living body structure, cylindrical micro-molded products were joined together by the adhesive force of the adhesive material.
This living body structure was white, and the degree of randomness was slightly less than that of Example 1, and the three-dimensional network-like pores were slightly less, but had characteristics such as being hard to break.

Example 6
A large number of cylindrical micromolded materials were produced by the same method as in Example 1. This material was fired in an oxygen atmosphere at a maximum temperature of 1100 ° C. to obtain a cylindrical micro-molded product. By spraying a 20% aqueous solution of collagen as an adhesive material on the micromolded product, the surface of the micromolded product was covered with the adhesive material. The micro-molded product covered with an adhesive on the surface was randomly assembled in a cube having a side of 0.5 cm. By pressing this assembled material lightly, the assembly was obtained in a state where the micro-molded products were joined together. This aggregate was baked in an argon atmosphere at a maximum temperature of 1100 ° C. to obtain a biological structure composed of an aggregate of micro-molded articles in which the direction of the through hole made of tricalcium phosphate was random in the three-dimensional direction. . In this biological structure, carbon obtained from an adhesive material was mediated by firing in an argon atmosphere, and cylindrical micro-molded products were joined to each other.
This living body structure was black and had a characteristic that the degree of randomness was slightly less than that of Example 1 and the number of three-dimensional network pores was slightly reduced, but it was difficult to break.

Example 7
Tricalcium phosphate (main material) 80 parts by mass
Collagen (binding material) 20 parts by mass
100 parts by mass of ion-exchanged water The above compound was kneaded and extruded to produce a large number of cylindrical micromolded materials having through-holes having a length of 2.0 mm, an outer diameter of 0.5 mm, and a pore diameter of 300 μm. The surface of the material was covered with an adhesive material by spraying a 20% aqueous solution of collagen (same as the binding material) on the material as an adhesive material. The material covered with an adhesive on the surface was randomly assembled in a cube having a side of 0.5 cm. By pressing the assembled materials lightly, the aggregates were obtained with the materials joined together. This aggregate is baked in an argon atmosphere at a maximum temperature of 1100 ° C., and a biological structure composed of aggregates of micro-molded products in which the direction of the through holes made of tricalcium phosphate and carbon is random in a three-dimensional direction. Obtained. This living body structure was baked in an argon atmosphere, and the carbon obtained from the binder and the adhesive was used as a medium, and the cylindrical micro-molded products were joined together.
This living body structure was black and had a characteristic that the degree of randomness was slightly less than that of Example 1 and the number of three-dimensional network pores was slightly reduced, but it was difficult to break.

Comparative Example 1
Tricalcium phosphate (main material) 80 parts by mass
Polyvinyl alcohol (binding material) 20 parts by mass
100 parts by mass of ion-exchanged water After kneading the above blend, a polyurethane foam having a side of 0.5 cm was immersed in the kneaded product, and the kneaded product was impregnated in the polyurethane foam. The polyurethane foam impregnated with the kneaded product was dried and then fired in an oxygen atmosphere at a maximum temperature of 1100 ° C. to obtain a three-dimensional network structure. The structure was cracked and weak in strength, and could not be used as a biological structure.

Comparative Example 2
Tricalcium phosphate (main material) 80 parts by mass
Polyvinyl alcohol (binding material) 20 parts by mass
100 parts by mass of ion-exchanged water The above blend was kneaded and extruded to produce a large number of micromolded materials.
The micromolded material was fired in an argon atmosphere at a maximum temperature of 1100 ° C., and then processed into a bead shape to obtain a substantially spherical micromolded product having a through hole having an outer diameter of about 1 mm and a hole diameter of 300 μm. The surface was covered with an adhesive material by spraying a 20% aqueous solution of polyvinyl alcohol (same as the binding material) as an adhesive material on the micro-molded product processed into a substantially spherical bead shape. The roughly spherical bead-shaped micro-molded product covered with an adhesive material on the surface is randomly assembled in a cube with one side of 0.5 cm, and the aggregate is formed in a state where the micro-molded products are joined by lightly pressing. Obtained. The joined micro-molded products were baked at a maximum temperature of 1100 ° C. in an argon atmosphere to obtain a biological structure. This biostructure was baked in an argon atmosphere, and the carbon obtained from the binder and the adhesive was used as a medium, and the micro-molded products were joined together.
This living body structure is black, and the micro-molded product has a bead shape, so it overlaps multiple layers, the randomness of the through holes is lost, and the three-dimensional network structure is also formed in the gap between the micro-molded products. No pores having had been obtained.

  The living body structure of the present invention promotes the formation of blood vessels and cell proliferation, and facilitates cell entry from multiple directions, and thus becomes a good bone filling material or cell culture carrier. Can be applied.

DESCRIPTION OF SYMBOLS 1 Structure for biological body 2 Micromolded article 3 Through-hole of micromolded article 2 4 Pore of structure 1 for biological body

Claims (6)

  At least the main material and the binder are kneaded and molded to produce a columnar micro-molded material having at least one through hole in the longitudinal direction, and the materials are assembled randomly in the three-dimensional direction to join the materials together A living body structure comprising an aggregate of micro-molded products obtained by drying or firing after being formed.   The living body structure according to claim 1, wherein the joining of the materials is performed by activating the surfaces of the materials.   The living body structure according to claim 1, wherein the bonding between the materials is performed by applying an adhesive material to the surfaces of the materials.   A columnar micromolded material obtained by kneading at least a main material and a binder and forming a columnar micromolded material having at least one through hole in the longitudinal direction and firing the material. A biological structure comprising an assembly of micro-molded products obtained by applying a surface with an adhesive material, randomly assembling them in a three-dimensional direction, joining the micro-molded products, and drying or firing.   5. The structure according to claim 1, wherein the living body structure is formed by randomly assembling the materials in a three-dimensional direction so as to have three-dimensional network pores between the materials of the micro-molded product. The biological structure according to item 1.   The biological structure according to any one of claims 1 to 5, wherein the main material is calcium phosphate.

Images