JP2010151472A - Observation stand of observation object and observation method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an observation method which enables the observation of the observation object contained in an observation sample solution from many aspects. <P>SOLUTION: In the observation method for observing the observation object by allowing the observation sample solution containing the observation object to flow in a predetermined direction, a pipe member 6 having a large number of microprotrusions 12 provided to at least a part of the inner peripheral surface thereof is used and a sample solution supply means 16 for supplying the observation sample solution is connected to one end side of the pipe member 6 while a sample solution recovery means 18 for recovering the observation sample solution is connected to the other end side of the pipe member 6. If the observation sample solution is allowed to flow to the sample solution recovery means 18 from the sample solution supply means 16 through the pipe member 6, the observation object contained in the observation sample solution collides with a large number of the minute protrusions 12 when the observation sample solution flows through the pipe member 6 and the observation object is revolved by utilizing the flow of the observation sample solution in the predetermined direction due to the collision. As a result, the observation object can be observed from many aspects using a microscope. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an observation table and an observation method for observing an observation body such as a somatic cell, a plant cell, a bacterium, or a virus.

  In order to observe an observation body such as a somatic cell, a preparation using a slide glass is used (for example, see Patent Document 1). This preparation, for example, drops one drop of the observation sample liquid containing the observation body onto a slide glass, and then slowly covers the observation sample liquid so that bubbles do not enter with the glass cover, and then absorbs the observation sample overflowing from the glass cover. However, it is formed by such a procedure.

Japanese Examined Patent Publication No. 7-69253

  In the observation using the above-described preparation, the observation object to be observed is held stationary between the slide glass and the cover glass, and the observation object held in this stationary state is observed with a microscope. In general, the observation object has a three-dimensional structure. However, in the observation using the above-described preparation, the observation object can be seen only in a plane, and therefore, only one specific surface of the observation object is observed. Therefore, it is strongly desired to realize an observation table and an observation method capable of viewing the observation body from various angles.

  An object of the present invention is to provide an observation table and an observation method capable of observing an observation body contained in an observation sample solution from multiple angles.

  The observation method for an observation body according to claim 1 of the present invention is an observation method for observing the observation body by flowing an observation sample liquid containing the observation body in a predetermined direction, and at least a part of the inner peripheral surface. A flow path member having an observation flow path provided with a large number of microprojections is connected to a sample liquid supply means for supplying the observation sample liquid to one end side of the observation flow path. A sample solution collecting means for collecting the observation sample solution is connected to the other end of the sample solution, and the observation sample solution is caused to flow in the predetermined direction from the sample solution supply means to the sample solution collecting means through the observation channel, The observation body contained in the observation sample liquid is caused to collide with the large number of minute protrusions when flowing through the observation flow path, and the observation body is moved using the flow in the predetermined direction of the observation sample liquid due to the collision. It is made to rotate.

  Moreover, in the observation method of the observation body according to claim 2 of the present invention, the flow path member is composed of a tube member having the observation flow path, and is placed so as to bridge the tube member to a slide glass, A cover glass is placed so as to cover the tube member, and the tube member is fixed by filling an encapsulant between the slide glass and the cover glass.

  Moreover, in the observation method of the observation body according to claim 3 of the present invention, the inner diameter of the tube member is 50 to 1000 μm, and the height of the many protrusions of the tube member is 0.5 to 10 μm. It is characterized by.

  In the observation method for an observation body according to claim 4 of the present invention, the observation sample liquid is stored in the pressure difference between the sample liquid supply means and the sample liquid recovery means or in the sample liquid supply means. Using the concentration difference between the observed sample liquid and the collected liquid stored in the sample collecting means, the sample liquid is supplied from the sample liquid supply means to the sample liquid collecting means through the tube member.

  Moreover, the observation stand for the observation body according to claim 5 of the present invention is an observation stand for observing the observation body by flowing an observation sample liquid including the observation body in a predetermined direction, and includes a slide glass, A flow path member disposed on the upper side of the slide glass, and a plurality of minute protrusions are provided on at least a part of the inner peripheral surface of the observation flow path of the flow path member.

Moreover, in the observation stand for an observation body according to claim 6 of the present invention, an encapsulant is filled between the flow path member and the slide glass.
Moreover, in the observation stand of the observation body according to claim 7 of the present invention, the flow path member is formed of a tube member, a cover glass is disposed on the upper side of the tube member, and the slide glass, the cover glass, The encapsulant is filled so as to cover the space between the tube members.

  Furthermore, in the observation stand for an observation body according to claim 8 of the present invention, the inner diameter of the tube member is 50 to 1000 μm, and the height of the plurality of protrusions of the tube member is 0.5 to 10 μm. It is characterized by.

  According to the observation method for an observation body according to claim 1 of the present invention, a flow path member having an observation flow path provided with a large number of microprojections on at least a part of the inner peripheral surface is used. A sample solution supply means is connected to one end side of the path, and a sample solution recovery means is connected to the other end side. An observation sample liquid containing an observation body (for example, a somatic cell such as a human body cell, a plant cell, a bacterium, or a virus) flows from the sample liquid supply means to the sample liquid recovery means through the observation flow path, and passes through the observation flow path. When flowing, the observation body contained in the observation sample liquid collides with a large number of microprojections in the observation flow path, and the collision causes the observation body to slowly rotate using the flow of the observation sample liquid. The observation body contained in the observation sample liquid can be observed from multiple sides.

  Moreover, according to the observation method for observing object according to claim 2 of the present invention, the tube member as the observation member is placed so as to bridge the slide glass, and the cover glass is placed so as to cover the tube member. Since the encapsulant is filled between the slide glass and the cover glass, the tube member can be fixed on the slide glass to facilitate its handling.

  Moreover, according to the observation method of the observation body according to claim 3 of the present invention, the inner diameter of the tube member is 50 to 1000 μm, and the height of many protrusions of the tube member is 0.5 to 10 μm. It can be applied conveniently for observing somatic cells, bacteria, viruses, etc. as an observing body. When it collides with a microprojection, the observation body is slowly rotated using the flow of the observing sample liquid. It becomes possible to do.

  In addition, according to the observation method for an observation body described in claim 4 of the present invention, the pressure difference between the sample liquid supply means and the sample liquid recovery means (or the observation sample liquid and the sample accommodated in the sample liquid supply means) Since the observation sample liquid is flowed using the difference in concentration with the collected liquid stored in the collecting means), the observation sample liquid can be flowed slowly little by little. Can be slowly rotated using the flow of the observation sample liquid when it collides with the microprojection.

  Moreover, according to the observation stand of the observation body according to claim 5 of the present invention, since the flow path having the observation flow path is disposed on the upper side of the slide glass, by flowing the observation sample liquid through the observation flow path, The observation body contained in the observation sample solution can be observed with a microscope. In addition, since a large number of minute protrusions are provided on at least a part of the inner peripheral surface of the observation channel, when the observation sample solution is flowed through the observation channel and observed, the observation object included in the observation sample solution is It collides with a large number of microprojections in the observation channel, and the collision causes the observation body to slowly rotate using the flow of the observation sample liquid. Therefore, the observation body contained in the observation sample liquid is It becomes possible to observe from multiple sides.

  Moreover, according to the observation stand according to claim 6 of the present invention, since the encapsulant is filled between the tube member as the flow path member and the slide glass, the tube member is fixed to the slide glass and observed. can do.

  Moreover, according to the observation stand according to claim 7 of the present invention, the cover glass is disposed on the upper side of the tube member, and the space between the cover glass and the slide glass is filled with the encapsulant. Can be securely fixed and the observation body can be observed as desired.

  Furthermore, according to the observation stand for an observation body according to claim 8 of the present invention, the inner diameter of the tube member is 50 to 1000 μm, and the height of the numerous projections of the tube member is 0.5 to 10 μm. The present invention can be advantageously applied to multifaceted observation of human body cells, bacteria, viruses and the like as observation bodies.

  Hereinafter, an observation table and an observation method for an observation body according to the present invention will be described with reference to the accompanying drawings. 1 is a perspective view showing an embodiment of an observation table according to the present invention, FIG. 2 is a sectional view taken along line II-II in FIG. 1, and FIG. 3 is a sectional view taken along line III-III in FIG. FIG. 4 is an enlarged explanatory view for explaining a mechanism of rotation of the observation body, FIG. 5 is a view for explaining a spraying process in manufacturing the tube member, and FIG. FIG. 7 is a diagram for explaining a heat welding process in the production of the pipe member, FIG. 7 is a diagram for explaining a preparation mounting process in the production of the pipe member, and FIG. 8 is an enclosure in the production of the pipe member. It is a figure for demonstrating an agent filling process.

  1 to 3, the illustrated observation table 2 includes a slide glass 4 serving as a base, a tube member 6 disposed on the upper side of the slide glass 4, and a cover glass disposed on the upper side of the tube member 6. 8 and the space between the slide glass 4 and the cover glass 8 is filled with the encapsulant 10. (In FIG. 1, the filler is omitted). The slide glass 4, the cover glass 4, and the tube member 6 are made of, for example, a transparent glass material, and commercially available slide glass 4 and cover glass 6 can be used.

  When the observation body P (see FIG. 4) is observed using the observation table 2, the observation sample liquid containing the observation body P flows through the tube member 6, and in this connection, the tube member 6 is as follows. Configured. A large number of minute protrusions 12 (see also FIG. 4) are provided on at least a part of the inner peripheral surface of the tube member 6. Such a minute protrusion 12 is formed of, for example, the same material as the tube member 6 (for example, a transparent glass material), and is integrated with the inner peripheral surface of the tube member 6 by heating and welding glass powder as will be described later. Can be provided.

  In this embodiment, a large number of minute protrusions 12 are provided substantially uniformly over the entire inner peripheral surface of the tube member 6 as the flow channel member, that is, the entire peripheral surface defining the observation flow channel 14. The cross-sectional shape inside the tube member 6 (that is, the cross-sectional shape of the peripheral surface that defines the flow path 14) is formed in a circular shape, and by using such a cross-sectional shape, observation is performed while flowing the observation sample liquid. The body P can be observed from multiple sides as will be described later. The inner cross-sectional shape of the tube member 6 (that is, the observation channel 14) may be elliptical, and by making such an elliptical shape, the thickness of the tube member 6 in a solid state on the slide glass 4 can be reduced. It can be made thin, and observation when observing using a microscope becomes easy.

  The inner diameter of the tube member 6 is preferably 50 to 1000 μm. By forming the tube member 6 in such a size, somatic cells (cell clumps), bacteria, viruses, and the like as the observation body P are observed in a multifaceted manner. Can be applied conveniently. The inner diameter of the tube member 6 (that is, the diameter of the observation channel 14) is appropriately selected according to the size of the observation body P to be observed, and is 2 to 3 times larger than the maximum of the observation body P. It is desirable to make it. In general, the maximum size of a cell clump observed using a microscope is about 300 μm, and when observing such a cell clump, the inner diameter is formed to be about 1000 μm. By doing so, the observation body P can be caused to collide with the minute protrusions 12 on the inner peripheral surface thereof as desired without clogging the tube member 6.

  The size of the large number of microprojections 12, that is, the projecting height projecting inward is preferably 0.5 to 10 μm, and by setting such a height, the observation object is caused to collide as described later. Can be rotated. If the protrusion height of the microprojection is lower than 0.5 μm, the observation object will hardly collide, and even if it collides, it will flow to the downstream side with little rotation, and the protrusion height will exceed 10 μm. These projections become a great resistance to the flow of the observation sample liquid.

  Such a tube member 6 is arranged on the upper side of the slide glass 4 so as to bridge in the lateral direction (the direction perpendicular to the longitudinal direction of the slide glass and from the upper left to the lower right in FIG. 1). The sample solution supply means 16 is connected to one side (left side in FIGS. 1 and 2), and the sample solution recovery means 18 is connected to the other side (right side in FIGS. 1 and 2). In this embodiment, the sample liquid supply means 16 and the sample liquid recovery means 18 are constituted by the droppers 20 and 22, and the tip 24 of the dropper 20 on the supply side is in one end of the tube member 6 (on one side of the observation channel 14). The distal end portion 26 of the dropper 22 on the collection side is inserted and connected into the other end of the tube member 6 (to the other side of the observation channel 14). In this case, as shown by an arrow 30 in FIG. The sample solution flows in a predetermined direction (rightward in FIG. 2) from the dropper 20 on the supply side through the tube member 6 (observation flow path 14) to the dropper 22 on the collection side. Contrary to the above, the sample liquid recovery means 18 (recovery side dropper 22) is connected to the one side of the tube member 6, and the sample liquid supply means 16 (supply side dropper 20) is connected to the other side. In such a case, the observation sample liquid flows in a direction opposite to the direction indicated by the arrow 30.

  The sample liquid supply means 16 is composed of a supply side container in which an observation sample liquid is accommodated, and the sample liquid recovery means 18 is composed of a recovery side container in which the observation sample liquid is recovered, and the supply side container is, for example, via a supply tube Connected to one end side of the pipe member 6, connected to the other end side of the pipe member 6 via a recovery tube, for example, and supplied from the supply side container using, for example, a feed pump (or gravity) The observation sample solution may be flowed to the collection side container through the tube, the pipe member, and the collection tube.

  As the observation sample solution, for example, an organic solvent can be used, and an encapsulant that is appropriately diluted with xylene can be used conveniently. As the observation test solution, it is necessary to select an organic solvent when the observation body P (for example, cell agglomeration) is stained with a dye and transparentized with an organic solvent after staining. When performing vital staining or the like and observing in an aqueous solution, an appropriate liquid such as water or alcohol can be used.

  The observation table 2 can be manufactured, for example, as shown in FIGS. In order to manufacture the tube member 6 of the observation table 2, for example, a glass capillary tube 32 (for example, a hematocrit tube) formed from a transparent glass material is used, and a glass powder 34 (for example, a cover glass 8) formed from a transparent glass material is used. Prepare a finely ground material). A glass powder having a size of about 0.5 to 10 μm is used. Then, for example, after the glass powder 34 is sucked into the dropper 36, the dropper 36 is pushed to spray the glass powder 34 in the dropper 36 into the glass capillary 32 as shown in FIG. Can be substantially uniformly attached to the inner peripheral surface of the glass capillary tube 32. For example, static electricity may be applied to the glass powder 34 and the static electricity may be applied to the inner peripheral surface of the glass capillary 32.

  Next, as shown in FIG. 6, the glass capillary 32 is heated to weld the glass powder 34 to the inner peripheral surface thereof. For example, a gas burner 38 is used for heating, and the gas burner 38 is moved in the axial direction indicated by the arrow 40 and rotated in the circumferential direction indicated by the arrow 42 so that the gas burner 38 is heated uniformly by the flame. When heated in this manner, the glass powder 34 is melted, and the melted glass powder 34 is welded to the inner peripheral surface of the glass capillary tube 32, and thus the tube member 6 having a large number of microprojections 12 can be manufactured. it can.

  Next, as shown in FIG. 7, the tube member 6 is disposed on the upper side of the slide glass 4, and the cover glass 8 is disposed on the upper side thereof. At this time, the tube member 6 is placed so as to bridge in the lateral direction (that is, so as to cross) in the longitudinal center of the slide glass 4, and the cover glass 8 is placed so as to cover the tube member 6. Is done.

  Thereafter, as shown in FIG. 8, the space between the slide glass 4 and the cover glass 8 is filled with an encapsulant 10, and the periphery of the tube member 8 is wrapped by the filler 10. For example, Canadian balsam or the like can be used as such an encapsulant 10, and the encapsulant 10 is dried and the tube member 6 is fixed. Thus, the observation table 2 shown in FIGS. Can be produced.

  Next, with reference to FIGS. 1 to 4, observation of the observation body P using the observation table 2 manufactured as described above will be described. At the time of observation, the observation table 2 is attached to an observation region of a microscope (not shown). Then, as shown in FIG. 2, the supply side dropper 20 containing the observation sample liquid containing the observation body P is connected to one side of the tube member 6, and the recovery side dropper 22 containing nothing is connected to the other side of the tube member 6. Connect to.

  In the state where the supply side and recovery side syringes 20 and 22 are connected in this way, the observation sample liquid in the supply side syringe 20 is slowly pressed on the supply side syringe 20 as shown by the arrow 30 (see FIG. 2). And then collected in the collection side syringe 22 (At this time, in order to make the flow of the observation sample liquid smooth, the collection side syringe 22 is slowly released from the pressed state).

  When the observation sample liquid is slowly flowed through the tube member 6 in this way, as shown in FIG. 4, a part of the observation body P contained in the observation sample liquid (specifically, the vicinity of the inner peripheral surface of the tube member 6 is (Observation body flowing in the direction indicated by the arrow 40) collides with the minute protrusion 12 on the inner peripheral surface of the tube member 6, and this collision stops movement in the direction indicated by the arrow 40 at the collision site in the observation body P. The flow in the direction indicated by the arrow 40 of the observation sample liquid acts at the site opposite to the site. As a result, the observation body P that has collided with the minute protrusion 12 rotates using the flow of the observation sample liquid, and the observation body P that flows in the vicinity of the upper side in FIG. As shown in FIG. 4, the observation body P that rotates in the counterclockwise direction in FIG. 4 and flows in the vicinity of the lower side in FIG. To come.

  Thus, since the observation body P moves downstream while rotating, the observation body P can be viewed in a multifaceted manner only by looking at a predetermined observation region with a microscope, and the observation body can be viewed with a simple method. It becomes possible to observe P in more detail.

  In this observation method, it is desirable to use a liquid having a certain degree of viscosity as the observation sample liquid. By using such a liquid, the flow of the observation sample liquid can be made slow and the rotation of the observation body P can be performed. The speed can also be decreased by the viscosity, and the detailed observation of the observation body P becomes easier. Note that the rotation speed due to the collision of the observation body P is affected by the flow rate and viscosity of the observation sample liquid, the inner diameter of the tube member 6, the size of the observation body P, and the like, so that the observation sample can be easily observed. The flow rate and viscosity of the liquid, the inner diameter of the tube member, and the like are appropriately set.

  In the observation method described above, the observation sample liquid in the supply side dropper 20 is collected in the collection side dropper 22 through the tube member 6, and when observation is performed using two droppers in this way, the observation in the supply side dropper 20 is observed. After flowing the sample liquid into the collection side dropper 22, the collection side dropper 22 is filled with the observation sample liquid, and the supply side dropper 20 is emptied. Next, the collection side dropper 22 is supplied to the supply side dropper. By using the side dropper 20 as the collection side dropper, the observation body P contained in the observation sample liquid can be repeatedly observed.

In this observation method, for example, an organic solvent can be used as the observation sample solution, and an encapsulating agent diluted with xylene can be conveniently used.
As described above, the embodiments of the observation table and the observation method for observing the observation body according to the present invention have been described. However, the present invention is not limited to such embodiments, and various embodiments can be made without departing from the scope of the present invention. Variations or modifications are possible.

  For example, in the observation table of the above-described embodiment, a tube member is disposed on the upper side of the slide glass, a cover glass is disposed on the upper side of the tube member, and an encapsulant is placed in a space between the slide glass and the cover glass. Although it was filled, it is not limited to such a form, You may make it arrange | position a tube member on the upper side of a slide glass, and you may make it fill with an encapsulant between a slide glass and a tube member (a cover glass was abbreviate | omitted). Or a tube member may be provided on the upper side of the slide glass (the cover glass and the encapsulant are omitted).

  In the above-described embodiment, the pipe member is used as the flow path member. However, the present invention is not limited to such a pipe member, and the observation flow path is formed on a plate-like or block-like member, and such a plate-like member is used. A member or a block-like member can be used as the flow path member.

  In the observation table of the above-described embodiment, a large number of minute protrusions are provided over the entire inner peripheral surface of the tube member. However, it is not necessary to provide the micro projections over the entire area, and provided over at least a part of the inner peripheral surface. The above-mentioned desired effect can be achieved. For example, in the upstream part of the pipe member (part on the sample liquid supply means side), a number of microprojections are provided on the upper inner peripheral surface (or lower inner peripheral surface), and the downstream side part (sample liquid recovery means) In the side portion, a large number of minute protrusions may be provided on the lower inner peripheral surface (or the upper inner peripheral surface).

  Further, for example, the observation body can be configured as shown in FIGS. 9 and 10 so as to rotate in four directions. 9 and 10, members substantially the same as those shown in FIGS. 1 to 8 are given the same reference numerals, and descriptions thereof are omitted.

  9 and 10, in the observation platform 2A of this other form, the tube member 6A as the flow path member is modified, and the inner circumferential surface of the tube member 6A is substantially equidistantly spaced in the circumferential direction. Four projecting regions 52a, 52b, 52c, and 52d are provided. Each protrusion region 52a, 52b, 52c, 52d is provided with a large number of minute protrusions 12, and these minute protrusions can be formed from glass powder as described later, as in the above-described embodiment. .

  In this embodiment, the pipe member 6A is composed of four portions 54a, 54b, 54c, 54d that are continuously provided in the axial direction (the direction from the lower left to the upper right in FIG. 9, the left-right direction in FIG. 10). Portions 54a, 54b, 54c. Projection regions 52a, 52b, 52c, and 52d corresponding to 54d are provided. When viewed from the lower left to the upper right in FIG. 9 (from the left to the right in FIG. 10), the portion 54a of the pipe member 6A has its The left side inner surface has a projecting region 52a on its portion 54b, its upper inner surface has a projecting region 52b, its portion 52c has its right inner surface on its right side, and the portion 54d has its lower inner surface. Protrusion regions 52d are provided at substantially 90 degree intervals in the circumferential direction.

  In this embodiment, four projecting regions 52a, 52b, 52c, and 52d are provided at intervals in the circumferential direction. However, the present invention is not limited to this, and there are two or three projecting regions in the circumferential direction. Five or more may be provided. In this embodiment, the protrusion regions 52a, 52b, 52c, and 52d are provided corresponding to the portions 54a, 54b, 54c, and 54d of the pipe member 6A. 52a, 52b, 52c, and 52d may be provided from one end to the other end of the pipe member 6A.

  The tube member 6A used for the observation table 2A shown in FIG. 9 can be manufactured, for example, as shown in FIG. First, as shown in FIG. 10A, an extremely fine wire 62 (for example, an extremely fine piano wire) is passed through a glass capillary tube 321, glass powder 64 is attached to the wire 62, and a portion with the glass powder 64 is attached. Position in the glass capillary 32. And the wire | line 62 is pressed on the inner surface of the glass capillary 32, and the glass powder 64 is made to adhere to the specific site | part of the internal peripheral surface from one end to the other end. On the contrary, the glass powder 64 may be attached to the wire 62, and the wire 62 with the glass powder 64 may be passed through the glass capillary 32.

  Thereafter, as shown in FIG. 10B, similarly to the above, heating is performed using, for example, a gas burner (not shown), and the attached glass powder is welded to the inner surface of the glass capillary tube 32. In this manner, the protruding region 66 is formed on a part of the inner peripheral surface of the glass capillary 32.

  Next, as shown in FIG. 10C, the glass capillary 32 is cut at the locations indicated by the alternate long and short dash lines 68a, 68b, 68c, and analyzed into four parts 54a, 54b, 54c, 54d.

  Thereafter, as shown in FIG. 10 (d), the portion 54b is positioned relative to the portion 54a so as to rotate 90 degrees clockwise as viewed from the left to the right in FIG. 10 (d). 54b is melt-bonded by heating or the like. Further, the portion 54c is positioned in a positional relationship rotated 90 degrees clockwise as viewed from the left to the right in FIG. 10D with respect to the portion 54b, and the portion 54c is melt bonded to the portion 54b. Similarly, the portion 54d is similarly positioned 90 ° clockwise and melted and joined to the portion 54c. Thus, the tube member 6A can be manufactured.

  The tube member 6A thus manufactured is placed on the slide glass 4 in the same manner as described above, the cover glass 8 is placed, and the encapsulating agent 10 is filled between the slide glass 4 and the cover glass 8 as shown in FIG. 2A can be manufactured.

The perspective view which shows one Embodiment of the observation stand according to this invention. Sectional drawing by the II-II line in FIG. Sectional drawing by the III-III line in FIG. The enlarged explanatory view for explaining the mechanism of rotation of an observation object. The figure for demonstrating the spraying process in manufacture of a pipe member. The figure for demonstrating the heat welding process in manufacture of a pipe member. The figure for demonstrating the preparation mounting process in manufacture of a pipe member. The figure for demonstrating the mounting agent filling process in manufacture of a pipe member. Sectional drawing which shows the observation stand of another form. The figure for demonstrating the process for manufacturing the observation stand of FIG.

Explanation of symbols

2,2A Observation table 4 Slide glass 6,6A Tube member 8 Cover glass 10 Encapsulant 12 Microprojection 16 Sample liquid supply means 18 Sample liquid recovery means 32 Glass capillary 34 Glass powder 52a, 52b, 52c, 52d Projection area P Observation body

Claims (8)

  An observation method for observing the observation body by flowing an observation sample liquid including the observation body in a predetermined direction, the flow comprising an observation flow path provided with a large number of microprojections on at least a part of an inner peripheral surface A sample liquid for connecting the sample liquid supply means for supplying the observation sample liquid to one end side of the observation flow path using a path member and for collecting the observation sample liquid to the other end side of the observation flow path The recovery means is connected, the observation sample liquid is allowed to flow in the predetermined direction from the sample liquid supply means to the sample liquid recovery means through the observation flow path, and is included in the observation sample liquid when flowing through the observation flow path A method for observing an observing object, wherein the observing object is caused to collide with the plurality of minute protrusions, and the observing object is rotated by using the flow of the observation sample liquid in the predetermined direction by the collision.   The flow path member is composed of a tube member having the observation flow path, the tube member is placed so as to bridge a slide glass, a cover glass is placed so as to cover the tube member, and the slide glass and The observation method according to claim 1, wherein the tube member is fixed by filling an encapsulating agent between the cover glass and the cover glass.   The observation method of the observation body according to claim 2, wherein the inner diameter of the tube member is 50 to 1000 µm, and the height of the many protrusions of the tube member is 0.5 to 10 µm.   The observation sample liquid includes a pressure difference between the sample liquid supply means and the sample liquid recovery means, or the observation sample liquid stored in the sample liquid supply means and a recovery liquid stored in the sample recovery means. 4. The observation method for an observation body according to claim 2, wherein the difference in concentration is used to flow from the sample solution supply means to the sample solution recovery means through the tube member.   An observation table for observing the observation body by flowing an observation sample liquid containing the observation body in a predetermined direction, comprising a slide glass, and a flow path member disposed on the upper side of the slide glass, An observation table for an observation body, wherein a plurality of minute protrusions are provided on at least a part of the inner peripheral surface of the observation channel of the channel member.   The observation table according to claim 5, wherein an encapsulant is filled between the flow path member and the slide glass.   The flow path member is composed of a pipe member, a cover glass is disposed on the upper side of the pipe member, and the space between the slide glass and the cover glass is filled with the encapsulant so as to cover the pipe member. The observation table for an observation body according to claim 6, wherein the observation table is an observation table.   The observation base for an observation body according to claim 7, wherein an inner diameter of the tube member is 50 to 1000 µm, and a height of the plurality of protrusions of the tube member is 0.5 to 10 µm.

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