JP2010115410A - Nerve regeneration base material - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a nerve regeneration base material suitable for efficiently regenerating relatively thin nerve fibers in autonomic nerves or the like in a correct direction without the need of special implements or operations. <P>SOLUTION: A nerve regeneration sheet 11 includes a bend part 14 extended along a short side direction 104, and an adhesive layer 31 provided along the short side direction 104 on a position separated from the bend part 14 on an inner surface 13 and capable of bonding the inner surface 13. Thus, the relatively thin nerve fiber in the autonomic nerve or the like is clamped between the nerve regeneration sheet 11 and regenerated easily without performing suture. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a nerve regeneration substrate made of a biodegradable material.

  Human nerves may be damaged by diseases or injuries, and nerve damage may not be cured by self-recovery. Such nerve damage can cause movement disorders and organ dysfunction. On the other hand, an operation for suturing a damaged nerve and a treatment for collecting and transplanting a nerve from another part of the self are performed.

  Patent Document 1 discloses a precursor of a tissue regeneration device in which a filamentous material is fixed in an inner space of a cylindrical body made of a biodegradable material.

  Patent Document 2 discloses a nerve regeneration induction tube in which a nerve regeneration induction path is formed by a sponge-like matrix or the like inside a tubular body made of a biodegradable material.

JP 2008-43597 A JP 2004-208808 A

  The tissue regeneration device and nerve regeneration induction tube disclosed in Patent Document 1 and Patent Document 2 are used as a base material for regenerating cord tissue such as nerves and tendons. However, there are places where the nerves do not necessarily have a streak, so a cylindrical body or a tubular body is not suitable for such nerves.

  For example, in motor nerves, nerve fibers run in a bundle to form a bundle, so when the nerve is torn, insert the broken end of the nerve into both ends of the tubular body or tubular body, respectively, and suture it. Can do. On the other hand, in the autonomic nerve, there are parts where nerve fibers branch thinly and spread radially, and when the nerve is torn, a plurality of radially spread nerve fibers are bundled into a cylindrical shape Inserting and suturing a body or tubular body is an extremely complicated operation.

  The present invention has been made in view of these circumstances, and an object thereof is a nerve regeneration group suitable for regenerating relatively thin nerve fibers in an autonomic nerve or the like without requiring a special instrument or operation. The purpose is to provide materials.

  The present invention is a nerve regeneration substrate comprising a sheet made of a biodegradable material and sandwiching a nerve to be regenerated. The sheet includes a bent portion extending along a first direction connecting the first end side and the second end side where the nerve tearing ends are respectively disposed, and an inner surface facing the sheet when the sheet is bent at the bent portion. And a first pressure-sensitive adhesive layer that is provided along the first direction at a position spaced apart from the bent portion and can adhere the inner surfaces facing each other.

  The nerve regeneration substrate according to the present invention is for joining the fractured ends of nerves by nerve regeneration. In particular, this nerve regeneration base material is suitably used for the purpose of joining the fractured ends of the autonomic nerves by nerve regeneration. In the present specification, nerve fibers may be simply referred to as nerves. Nerve tears are not limited to the cause of the tear, but are severed or injured due to injury or disease, tears or defects associated with the removal of organs or tissues, and nerve cutting for the purpose of nerve transplantation. A wide variety of causes are included.

  The sheet has a thin sheet shape made of a biodegradable material. The planar shape of the sheet is not particularly limited, and a rectangular shape, a circular shape, an elliptical shape, a cloud shape, or the like can be adopted, but a rectangular shape is preferable for the convenience of packaging and work. Although the size of the sheet is not particularly limited, for example, if it is a rectangular shape used for regeneration of autonomic nerves in the abdominal cavity, each dimension of length × width × thickness is 10 to 100 mm × 10 to 100 mm × 0.01 to The thing of about 5.0 mm is suitable. More desirably, the first sheet and the second sheet have a length × width × thickness of about 15 to 30 mm × 15 to 30 mm × 0.1 to 2.0 mm.

  A biodegradable material is a material that can be decomposed in vivo, and preferably can be absorbed after decomposition. Examples of the biodegradable material include polylactic acid, polyglycolic acid, ε-aminocaprolactone, collagen, and chitosan. Among these, collagen is preferable from the viewpoint that an inflammatory reaction does not occur in humans and the rate of degradation and absorption by a living body can be controlled by a crosslinking treatment or the like.

  Collagen is a major protein component constituting the connective tissue of animals, and the main chain structure of the molecule is (Gly-XY), (Gly-Pro-X) and (Gly-Pro-Hyp). It means what is composed. Here, X and Y are natural or non-natural amino acids other than glycine, proline and hydroxyproline.

  Examples of collagen types include type I, type II, type III and type IV. Among these, from the viewpoint of easy handling, type I and type III collagen is preferred, but the type of collagen is not particularly limited in the present invention. In addition, the collagen in the present invention includes gelatin which is heat-denatured collagen, but is preferably not heat-denatured from the viewpoint of cell adhesion.

  Collagen is produced by extraction from living tissue, chemical polypeptide synthesis, recombinant DNA method, and the like. Among these, from the viewpoint of production cost, collagen produced by extraction from living tissue is preferable. In addition, examples of the origin of biological tissue include cattle, pigs, rabbits, sheep, mice, birds, fish, and humans. Examples of biological tissues include the aforementioned animal and human skin, tendons, bones, cartilage, and organs. Needless to say, the selection of the biological tissue and the origin is appropriately performed by those skilled in the art, and the present invention is not limited to the collagen derived from the biological tissue exemplified in the present specification.

  Moreover, as a collagen, what was processed so that it can melt | dissolve in a solvent is preferable from a viewpoint of making industrial manufacture easy. Examples of such collagen include solubilized collagen such as enzyme-solubilized collagen, acid-solubilized collagen, alkali-solubilized collagen, and neutral-solubilized collagen. Of these, acid-solubilized collagen is particularly preferable from the viewpoint of easy handling. Furthermore, from the viewpoint of safety when implanted in a living body, atelocollagen that has been subjected to the removal treatment of the telopeptide that is an antigenic determinant is preferable.

  As the sheet in the present invention, a film, a woven fabric, a non-woven fabric or the like prepared using a biodegradable material as a raw material is used. The sheet is composed of the above-described collagen fiber material (also simply referred to as “collagen fiber”). Of these, a woven fabric or a non-woven fabric of collagen fibers is particularly preferable.

  Examples of the method for producing a collagen fiber include a wet spinning method, a dry spinning method, and a melt spinning method. The wet spinning method is preferable because the production is easy and the production cost is low. . As a wet spinning method for obtaining such collagen fibers, known spinning methods (JP 2000-93497 A, JP 2000-210376 A, JP 2000-271207 A, etc.) can be employed.

  As a non-woven fabric made of collagen fibers, for example, a first layer in which a plurality of collagen fibers are arranged in parallel is laminated with a second layer in which a plurality of collagen fibers are arranged in parallel by changing the arrangement direction of the collagen fibers. In addition, the relationship between the first layer and the second layer is similarly repeated, and a plurality of layers are laminated and bonded to each other. As a method for producing such a nonwoven fabric composed of collagen fibers, the method disclosed in Japanese Patent Application Laid-Open No. 2003-301362 can be employed.

  Examples of the knitted fabric made of collagen fibers include woven fabrics and knitted fabrics. Examples of the woven fabric include those woven by plain weave and twill weave using collagen fibers. Examples of the knitted fabric include those woven by flat knitting or rubber knitting using collagen fibers.

  The sheet made of collagen fibers as described above is preferably a non-woven fabric of collagen fibers. In the non-woven fabric of collagen fibers, as described above, since the layers in which a plurality of collagen fibers are arranged in parallel are laminated, the first end side where the nerve tearing ends are respectively arranged on the inner surface of the sheet This is because the nerves are regenerated along the collagen fibers arranged on the inner surface of the sheet by laminating the plurality of collagen fibers in parallel along the first direction connecting the first end side and the second end side. That is, the regenerated nerve extends along the first direction with a guidance path between a plurality of collagen fibers.

  The first end side and the second end side on the inner surface of the sheet are positions where the tearing ends of the nerves face each other, and are generally near a pair of opposite ends on the inner surface of the sheet. is there. The nerve to be regenerated extends from the center of the torn end of the torn nerve fiber. Whether the central tear edge or the distal tear edge is positioned on the first end side or the second end side is determined in consideration of the specification of such nerve regeneration. When the sheet is a rectangle, a pair of short sides or the vicinity of the long sides facing each other in the rectangle is the first end side and the second end side. And the 1st direction which connects the 1st end side and the 2nd end side is a pair of short sides which do not become the 1st end side and the 2nd end side among a pair of short sides or long sides which oppose in the rectangle. Or the direction along the long side. In the present specification, “direction” includes opposite directions. Accordingly, the first direction includes both the direction from the first end side to the second end side and the direction from the second end side to the first end side.

  The sheet obtained as described above may be further subjected to various known physical or chemical crosslinking treatments. The stage at which this crosslinking treatment is performed is not particularly limited. Two or more crosslinking treatments may be used, and the order thereof is not limited.

  The crosslinking treatment described above can significantly delay the time taken for decomposition and absorption when the nerve regeneration substrate is implanted in the living body compared to the uncrosslinked material. This is useful in that the physical strength of the material is improved. That is, it becomes easy to maintain the necessary physical strength during the period until the portion where the nerve is lost is reproduced using the nerve regeneration substrate.

  Examples of the physical crosslinking described above include γ-ray irradiation, ultraviolet irradiation, electron beam irradiation, plasma irradiation, and crosslinking treatment by thermal dehydration reaction. Examples of chemical crosslinking methods include reactions with aldehydes such as dialdehyde and polyaldehyde, epoxies, carbodiimides, isocyanates, tannin treatment, chromium treatment, and the like.

  In addition, the sheet obtained as described above may be coated with a biodegradable substance. Examples of biodegradable substances used for coating include collagen and hyaluronic acid. Further, the sheet may be impregnated with various growth factors, drugs and the like. Nerve regeneration can be promoted by the action of these growth factors and drugs.

  The sheet is provided with a bent portion extending along a first direction connecting the first end side and the second end side. By this bent portion, the sheet is folded in half with the inner surface facing each other. The position of the bent portion is not particularly limited. For example, in the case of a quadrilateral sheet, if the bent portion is provided so as to connect the centers of a pair of opposing sides, the edges of the folded sheet overlap, This is preferable because a region where the gap is sandwiched can be widened.

  The bent portion described above is formed by easily folding the sheet and bonding the two sheets. For example, as described above, if a sheet is obtained as a non-woven fabric of collagen fibers, after laminating each layer in which a plurality of collagen fibers are arranged in parallel, it is folded and brazed to form a bent portion, and then The layers are bonded to each other and further subjected to a crosslinking treatment or the like as necessary to form a bent portion that is easily bent. Further, the brazing may be performed after the phases of the collagen fibers are bonded to each other.

  On the other hand, as described above, two sheets made of a non-woven fabric of collagen fibers are formed, and only predetermined edges of the two sheets are overlapped and bonded with a biological adhesive or the like. By binding the sheets into one sheet, the bound edge becomes a bent portion.

  The sheet is provided with a first adhesive layer along the first direction at a position away from the bent portion on the inner surface facing the sheet when the sheet is bent at the bent portion. This 1st adhesion layer can adhere | attach the mutually opposing inner surface.

  The first adhesive layer may be provided on the inner surface at a position separated from the bent portion. However, as described above, if the sheet is folded in two at the bent portion, the edges of the sheets overlap. If the first adhesive layer is provided in the vicinity of the edge that is in a position facing the bent portion in the folded state, it is preferable because a space in which nerve regeneration is promoted can be widened. In other words, the inner surfaces of the folded sheet are bonded to each other by the first adhesive layer to form a cylindrical shape, and the inner space of the cylindrical shape is a region where nerves are regenerated.

  A 1st adhesion layer is a layer which has the adhesive performance which can adhere | attach the inner surfaces of a sheet | seat, The substance which has such an adhesive performance is laminated | stacked on an inner surface, or a part of inner surface is processed and it exhibits adhesive performance. Can be formed. In addition, the first adhesive layer only needs to be provided on at least one of the inner surfaces facing each other when the first adhesive layer is folded in two by the bent portion, but the first adhesive layer may be provided on any of the opposing inner surfaces. Good.

  Specifically, the aspect which apply | coats the biomedical adhesive to the inner surface of a sheet | seat as a 1st adhesion layer is mention | raise | lifted. A bioadhesive is a material that can be decomposed in vivo, and preferably can be absorbed after decomposition. Examples of the biomedical adhesive include those mainly composed of fibrin using a blood coagulation reaction.

  The inner surface of the sheet is preferably provided with a second adhesive layer or a third adhesive layer on the first end side and the second end side, respectively, for fixing the fractured end of the nerve.

  The second adhesive layer and the third adhesive layer are layers having adhesive performance capable of adhering nerves to the inner surface of the sheet, and a substance having such adhesive performance is laminated on the inner surface or a part of the inner surface is processed. It can be formed by exhibiting adhesive performance. In addition, the second adhesive layer and the third adhesive layer may be provided on at least one of the inner surfaces that face each other when folded in two by the bent portion. A third adhesive layer may be provided.

  Specifically, the aspect which apply | coats the biomedical adhesive to the inner surface of a sheet | seat as a 2nd adhesion layer and a 3rd adhesion layer is mention | raise | lifted. A bioadhesive is a material that can be decomposed in vivo, and preferably can be absorbed after decomposition. Examples of the biomedical adhesive include those mainly composed of fibrin using a blood coagulation reaction.

  The nerve regeneration substrate according to the present invention is preferably sterilized by a known method such as γ-ray sterilization or ultraviolet sterilization before being used for medical purposes.

  According to the present invention, a bent portion extending along a first direction connecting a first end side and a second end side at which nerve tearing ends are respectively arranged on a sheet sandwiching a nerve to be reproduced, and the bent portion. And the first adhesive layer provided along the first direction and capable of adhering the opposing inner surfaces at a position spaced apart from the bent portion on the opposing inner surface when the sheet is bent at The relatively thin nerve fibers in the above can be easily sandwiched between the sheets and regenerated without being sutured.

  In addition, since the second adhesive layer or the third adhesive layer is provided on the inner surface of the sheet on the first end side and the second end side, respectively, for fixing the fractured end of the nerve, comparison in the autonomic nerve or the like The target nerve fiber can be easily fixed to the inner surface of the sheet without using sutures.

  Hereinafter, preferred embodiments of the present invention will be described. In addition, this embodiment is only one embodiment of this invention, and it cannot be overemphasized that an embodiment can be changed in the range which does not change the summary of this invention.

[Explanation of drawings]
FIG. 1 is a perspective view showing an external configuration of a nerve regeneration sheet 11 according to an embodiment of the present invention. FIG. 2 is a schematic diagram showing a method for producing a nonwoven fabric of collagen thread 21. FIG. 3 is a schematic diagram showing a method for regenerating the autonomic nerve 40 using the nerve regeneration sheet 11. FIG. 4 is a perspective view showing an external configuration of the nerve regeneration sheet 11 according to the modification.

[Nerve regeneration sheet 11]
The nerve regeneration substrate according to the present embodiment includes a single nerve regeneration sheet 11. As shown in FIG. 1, the nerve regeneration sheet 11 is a thin rectangular sheet. The nerve regeneration sheet 11 is bent at the bent portion 14, and the nerve tearing end is sandwiched between the nerve regeneration sheets 11 to promote nerve regeneration.

  The nerve regeneration sheet 11 is a nonwoven fabric of collagen thread 21. In detail, the collagen thread 21 is wound around the winding plate 20 while producing the collagen thread 21 by the wet spinning method. The winding plate 20 is a rectangular flat plate that is substantially the same as the nerve regeneration sheet 11. In winding the collagen thread 21, as shown in FIG. 2A, the direction 101 in which the collagen thread 21 is supplied and the rotation axis 102 of the winding plate 20 are orthogonal to each other.

  The winding plate 20 is inclined so that the long side direction 103 of the winding plate 20 intersects with the rotation axis 102 and is rotated with respect to the rotation axis 102, so that the collagen filament 21 becomes the long side of the winding plate 20. The film is wound on the winding plate 20 at an angle not orthogonal to the direction 103. Further, the winding plate 20 is slid in one direction relative to the collagen filament 21 along the rotation axis 102 while being rotated. This slide speed is set in consideration of the arrangement pitch of the collagen thread 21. As a result, the collagen filaments 21 are wound in parallel on the winding plate 20 from one end side to the other end side in the long side direction 103 of the winding plate 20, and the collagen filaments 21 are arranged in a certain direction. Is produced.

  Next, as shown in FIG. 2B, the inclination angle between the long side direction 103 and the rotation axis 102 in the winding plate 20 is changed, and the winding plate 20 is rotated with respect to the rotation axis 102. As a result, the collagen filament 21 is at an angle that is not orthogonal to the long side direction 103 of the take-up plate 20 and is different from the arrangement direction of the collagen filament 21 that has been previously taken up. It is wound up. Further, the winding plate 20 is slid in one direction relative to the collagen filament 21 along the rotation axis 102 while being rotated. As a result, the collagen filament 21 is wound in parallel on the winding plate 20 from the other end side in the long side direction 103 of the winding plate 20 to the one end side. A layer of the collagen thread 21 is laminated.

  As described above, the inclination angle between the long side direction 103 and the rotation axis 102 in the winding plate 20 is changed every time the collagen thread 21 is laminated, and the collagen thread 21 is wound on the winding plate 20. It is done. Finally, as shown in FIG. 2C, the long side direction 103 of the winding plate 20 and the rotation axis 102 are made parallel to each other, and the winding plate 20 is rotated with respect to the rotation axis 102. . Thereby, the collagen filament 21 is wound around the winding plate 20 along a direction (short side direction) orthogonal to the long side direction 103 of the winding plate 20. The winding plate 20 is slid in one direction relative to the collagen filament 21 along the rotation axis 102 while being rotated. Thereby, the layer of the collagen thread 21 arranged in the direction orthogonal to the long side direction 103 in the winding plate 20 is laminated on the outermost side of the winding plate 20. This collagen thread 21 corresponds to the collagen fiber in the present invention.

  As described above, the collagen filamentous material 21 wound on the winding plate 20 is dried, and then cut along the periphery of the winding plate 20 to obtain two collagen nonwoven fabrics. In these two collagen nonwoven fabrics, the outermost surface of the winding plate 20 is the inner surface 13 of the nerve regeneration sheet 11. Two collagen nonwoven fabrics are each cut | judged to the rectangle of a desired size as needed. Thereby, two collagen nonwoven fabrics as two nerve regeneration sheets 11 are obtained. In addition, although the method of producing two collagen nonwoven fabrics is described in the present embodiment, it goes without saying that the nerve regeneration base material according to the present invention is not limited to one produced as a set of two sheets.

  The obtained collagen non-woven fabric is bent once along a bent portion 14 to be described later, and then subjected to thermal dehydration crosslinking reaction, and further impregnated with an aqueous collagen solution to perform thermal dehydration crosslinking reaction again, whereby the membrane-like nerve regeneration sheet 11 is formed. can get.

  As described above, the collagen filaments 21 laminated on the outermost side of the winding plate 20 are along the direction (short side direction) orthogonal to the long side direction 103 of the winding plate 20, so that the nerve regeneration sheet 11, the collagen filaments 21 are arranged along the short side direction 104 of the nerve regeneration sheet 11. The collagen filament 21 extends over the long sides 15 and 16 of the nerve regeneration sheet 11.

[Bent part 14]
The nerve regeneration sheet 11 is provided with a bent portion 14 along the short side direction 104 over the long sides 15 and 16. By the bent portion 14, the nerve regeneration sheet 11 is folded in half with the inner surface 13 facing. The bent portion 14 is formed along a straight line connecting the centers of the long sides 105 in the long sides 15 and 16. The short side direction 104 corresponds to the first direction in the present invention.

  As described above, when the collagen nonwoven fabric is produced from the collagen thread 21, the bent portion 14 is bent and brazed to form the bent portion 14, and then the layers are mutually bonded by thermal dehydration crosslinking or the like. It can be easily bent by adhering to.

[Adhesive layers 31-33]
Three adhesive layers 31, 32, 33 are formed on the inner surface 13 of the nerve regeneration sheet 11.

  The pressure-sensitive adhesive layer 31 is a layer having an adhesive performance capable of bonding the inner surfaces 13 of the nerve regeneration sheet 11, and is formed by applying fibrin glue on the inner surface 13. The adhesive layer 31 is provided along the short side direction 104 in the vicinity of one short side 17 on the inner surface 13 of the nerve regeneration sheet 11. The adhesive layer 31 is an elongated region having the short side direction 104 as the longitudinal direction, and both ends in the longitudinal direction extend to the vicinity of the long sides 15 and 16. The adhesive layer 31 and the bent portion 14 are arranged to be separated in the long side direction 105. None of the adhesive layers 31 to 33 exists between the adhesive layer 31 and the bent portion 14. This adhesive layer 31 corresponds to the first adhesive layer in the present invention.

  The adhesive layers 32 and 33 are layers having adhesive performance capable of adhering the inner surface 13 of the nerve regeneration sheet 11 and the nerve tear edge, and are formed by applying fibrin glue on the inner surface 13.

  The adhesive layers 32 and 33 are each provided along the long side direction 105 on the inner surface 13 of the nerve regeneration sheet 11. Each of the adhesive layers 32 and 33 is an elongated region having the long side direction 105 as a longitudinal direction, and one of both ends in the longitudinal direction extends to the vicinity of the adhesive layer 31 and the other extends to the vicinity of the bent portion 14. ing. In other words, the adhesive layers 32 and 33 are provided across the adhesive layer 31 and the bent portion 14. Each of the adhesive layer 33 and the adhesive layer 34 is disposed in the vicinity of the long side 15 or the long side 16. The vicinity of the long sides 15 and 16 is a position where a nerve tearing end to be reproduced is disposed, and corresponds to the first end side and the second end side in the present invention, respectively. The adhesive layers 32 and 33 correspond to the second adhesive layer and the third adhesive layer in the present invention, respectively.

[How to use nerve regeneration sheet 11]
Hereinafter, as a method of using the nerve regeneration sheet 11, a method of joining the torn ends 41 and 42 of the autonomic nerve 40 will be described.

  First, as shown in FIG. 3A, the nerve regeneration sheet 11 is arranged in correspondence with the torn ends 41 and 42 of the autonomic nerve 40. At this time, the nerve regeneration sheet 11 is in the vicinity of the long sides 15 and 16 on the inner surface 13 side, and the tear edges 41 and 42 are disposed between the short side 17 and the bent portion 14, respectively. , 42 are separated in the short side direction 104 of the nerve regeneration sheet 11. Since the adhesive layers 32 and 33 are formed in the vicinity of the long sides 15 and 16, the tearing ends 41 and 42 are bonded to the inner surface 13 by the adhesive layers 32 and 33. At that time, the nerve tips at the respective tearing ends 41, 42 are positioned closer to the center of the inner surface 13 than the respective adhesive layers 32, 33.

  Subsequently, as shown in FIG. 3B, the nerve regeneration sheet 11 is folded in two by the bent portion 14 and overlapped so that the torn ends 41 and 42 of the autonomic nerve 40 are sandwiched by the nerve regeneration sheet 11. . Thereby, the inner surfaces 13 of the nerve regeneration sheet 11 are opposed to each other, and the short sides 17 and 18 are overlapped. Since the adhesive layer 31 is formed along the short side 17 on the inner surface 13 of the nerve regeneration sheet 11, the nerve regeneration sheet 11 folded in the vicinity of the short sides 17 and 18 is pressed so as to be sandwiched. Thus, the nerve regeneration sheet 11 folded in half is bonded in the vicinity of the short sides 17 and 18. As a result, it is possible to prevent peripheral tissues and the like from entering the inner surface 13 side of the nerve regeneration sheet 11.

  When left in the state described above, the nerve is regenerated from the torn ends 41 and 42 of the autonomic nerve 40, and the nerve between the torn ends 41 and 42 is recovered. As described above, since the collagen thread 21 is arranged along the short side direction 104 on the inner surface 13 of the nerve regeneration sheet 11, it is regenerated from the tear edges 41 and 42 along the collagen thread 21. Nervous nerves extend. That is, the regenerated nerve extends between the plurality of collagen filaments 21 as a guide route.

[Operational effects of this embodiment]
As described above, the nerve regeneration sheet 11 is provided along the short side direction 104 at the bent portion 14 extending along the short side direction 104 and the inner surface 13 at a position separated from the bent portion 14 in the long side direction 105. Therefore, relatively thin nerve fibers in the autonomic nerve 40 can be easily sandwiched and reproduced between the nerve regeneration sheets 11 without using sutures.

  Further, since the inner surface 13 of the nerve regeneration sheet 11 is provided with adhesive layers 32 and 33 for fixing the fractured ends 41 and 42 of the autonomic nerve 40 near the long sides 15 and 16, respectively. The relatively thin nerve fibers at 40 can be easily fixed to the inner surface 13 of the nerve regeneration sheet 11 without using suturing.

  In addition, since the bent portion 14 is provided so as to connect the centers of the pair of long sides 15 and 16 in the rectangular nerve regeneration sheet 11, the pair of short sides 17 and 18 of the nerve regeneration sheet 11 folded in half is provided. There is an advantage that the region where the autonomic nerve 40 is sandwiched is wide.

  Moreover, since the adhesive layer 31 is provided in the vicinity of the short side 17 facing the bent portion 14 in a state where the nerve regeneration sheet 11 is folded in two, there is an advantage that a space for promoting regeneration of the autonomic nerve 40 is wide. .

[Modification]
Hereinafter, modifications of the above-described embodiment will be described. In the modification, the configuration of the bent portion 14 in the nerve regeneration sheet 11 according to the above-described embodiment is different. Therefore, the detailed description about the bending part 19 is made below, and detailed description is abbreviate | omitted about the same structures, such as the adhesion layers 31-33. In FIG. 4, the parts denoted by the same reference numerals as those in the above-described embodiment indicate the same configuration.

  As shown in FIG. 4, in the modification, a bent portion 19 is formed on the nerve regeneration sheet 11 instead of the bent portion 14. The bent portion 19 is formed by overlapping and bonding only the edges of two sheets made of a collagen nonwoven fabric.

  After obtaining the collagen nonwoven fabric as described above, one collagen nonwoven fabric is cut into two equal parts at the position corresponding to the center in the long side direction 103 of the winding plate 20. The cut collagen nonwoven fabric is subjected to thermal dehydration cross-linking reaction, and further impregnated with a collagen aqueous solution, and then subjected to thermal dehydration cross-linking reaction again to obtain two sheets. These two sheets are overlapped so that the inner surfaces 13 thereof face each other, and in this state, only the edges are bonded with fibrin glue to form one nerve regeneration sheet 11. That is, two sheets are bound at the edge to form one nerve regeneration sheet 11. A bonded portion in the one nerve regeneration sheet 11 becomes a bent portion 19.

  As shown in FIG. 4, the bent portion 19 extends along the short side direction 104 of the nerve regeneration sheet 11. Since the two sheets bound as one nerve regeneration sheet 11 are each extended so as to form an acute angle at the bent portion 19, a state in which the bent portion 19 is easily folded in two is maintained. When the nerve regeneration sheet 11 is used, the inner surfaces 13 of the two bound sheets are opened so as to form a flat surface, and the rupture ends 41 and 42 of the autonomic nerve 40 are disposed therebetween. Such a nerve regeneration sheet 11 also provides the same operational effects as described above.

  In the above-described embodiment and modification, the adhesive layers 31 to 33 are provided on the inner surface 13 of the nerve regeneration sheet 11 only on the short side 17 side from the bent portions 14 and 19, but the adhesive layers 31 to 33 are provided. For example, it may be provided only on the short side 18 side from the bent portions 14 and 19 or may be provided on the short side 17 and 18 side in the same manner.

FIG. 1 is a perspective view showing an external configuration of a nerve regeneration sheet 11 according to an embodiment of the present invention. FIG. 2 is a schematic view showing a method for producing a nonwoven fabric of collagen thread 21. FIG. 3 is a schematic diagram showing a method for regenerating the autonomic nerve 40 using the nerve regeneration sheet 11. FIG. 4 is a perspective view showing an external configuration of the nerve regeneration sheet 11 according to the modification.

Explanation of symbols

11 ... Nerve regeneration sheet (nerve regeneration substrate)
13 ... inner surface 14, 19 ... bent portion 15, 16 ... short side (first end side, second end side)
21 ... Collagen filament (collagen fiber)
31 ... Adhesive layer (first adhesive layer)
32, 33 ... adhesive layer (second adhesive layer, third adhesive layer)
40 ... Autonomic nerves 41 and 42 ... Rupture end 104 ... Short side direction (first direction)

Claims (8)

A nerve regeneration base material comprising a sheet made of a biodegradable material and sandwiching a nerve to be regenerated,
The above sheet
A bent portion extending along a first direction connecting the first end side and the second end side where the fractured ends of the nerve are respectively disposed;
When the sheet is bent at the bent portion, the first adhesive is provided along the first direction at a position separated from the bent portion on the inner surface facing each other, and can adhere the inner surfaces facing each other. A nerve regeneration substrate having a layer.   2. The nerve regeneration substrate according to claim 1, wherein a second adhesive layer or a third adhesive layer is provided on the inner surface on the first end side and the second end side, respectively, for fixing a tear end of a nerve.   The nerve regeneration substrate according to claim 1 or 2, wherein the sheet is a film, a woven fabric or a non-woven fabric.   The nerve regeneration substrate according to any one of claims 1 to 3, wherein the sheet is composed of collagen fibers. The sheet is a single woven or non-woven fabric,
The nerve regeneration substrate according to claim 3 or 4, wherein the bent portion is a knitted fabric or non-woven fabric that is easily bent.   The nerve regeneration substrate according to claim 3 or 4, wherein the sheet is obtained by adhering at least two woven or non-woven fabrics at the bent portion.   The nerve regeneration substrate according to any one of claims 1 to 6, wherein the adhesive layer is made of a bioadhesive.   The nerve regeneration substrate according to claim 7, wherein the bioadhesive is fibrin.

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