JP2010039262A5 - - Google Patents
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- JP2010039262A5 JP2010039262A5 JP2008202911A JP2008202911A JP2010039262A5 JP 2010039262 A5 JP2010039262 A5 JP 2010039262A5 JP 2008202911 A JP2008202911 A JP 2008202911A JP 2008202911 A JP2008202911 A JP 2008202911A JP 2010039262 A5 JP2010039262 A5 JP 2010039262A5
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第１の発明の観察装置は、明部と暗部とを交互に設けてなる面光源を有し、前記面光源により被観察物を透過照明する照明手段と、前記照明手段により照明された前記被観察物からの射出光を結像する結像光学系と、前記結像光学系が形成した前記被観察物の光像を取得する撮像手段と、前記照明手段の透過照明光が前記被観察物を透過することで変形された前記面光源の明暗部の光像を補正する照明光補正手段と、前記照明光補正手段により補正された前記面光源の明暗部の位相をシフトさせながら前記撮像手段に複数の光像を取得し、前記複数の光像から合成画像を生成する画像合成手段と、を有することを特徴とする。 Observation apparatus of the first invention has a surface light source formed by providing a light and dark portions are alternately illuminating means for transmission illumination the I Rikomu observation object to the surface light source, illuminated by the illuminating means an imaging optical system for imaging light emitted from the object under observation, an imaging unit for obtaining an optical image of said object under observation which imaging optical system is formed, the transmissive illumination light is the object of the illumination means Illumination light correction means for correcting the light image of the bright and dark portions of the surface light source deformed by transmitting the observation object, and the phase of the light and dark portions of the surface light source corrected by the illumination light correction means while shifting And image synthesizing means for acquiring a plurality of light images in the imaging means and generating a composite image from the plurality of light images .
第２の発明の観察装置は、第１の発明の観察装置において、前記補正手段は、前記撮像手段で取得された光像の明暗部が直線になるように前記面光源の照明状態を補正することを特徴とする。 Observation apparatus according to the second invention, in the observation apparatus of the first aspect of the invention, the correction means, light dark light image acquired by the imaging means to correct the lighting conditions of the surface light source so that a straight line It is characterized by that.
第３の発明の観察装置は、第２の発明の観察装置において、前記補正手段は、前記透明容器に関する情報に基づいて、前記面光源の照明状態を補正し、前記透明容器に関する情報は、透明容器の種類、透明容器の材質、培養液の種類、培養液の量であることを特徴とする。 The observation device according to a third aspect is the observation device according to the second aspect, wherein the correction means corrects an illumination state of the surface light source based on information about the transparent container, and information about the transparent container is transparent. It is characterized by the type of the container, the material of the transparent container, the type of the culture solution, and the amount of the culture solution.
第４の発明の観察装置は、第２の発明の観察装置において、前記補正手段は、前記撮像手段で取得した前記被観察物の光像の画像から前記明暗部の形状変化を求め、前記撮像手段の画素ごとの歪補正率を求め、前記面光源の照明状態を補正し、前記面光源は、透過型液晶パネルであることを特徴とする。 The observation device according to a fourth aspect is the observation device according to the second aspect , wherein the correction means obtains a shape change of the bright and dark part from an image of the optical image of the observation object acquired by the imaging means, and the imaging The distortion correction factor for each pixel of the means is obtained, the illumination state of the surface light source is corrected, and the surface light source is a transmissive liquid crystal panel .
第５の発明の観察装置は、明部と暗部とを交互に設けてなる面光源により被観察物を透過照明する照明手段と、前記照明手段により照明された前記被観察物からの射出光を結像する結像光学系と、前記結像光学系が形成した前記被観察物の画像を取得する撮像手段と、前記面光源の明暗の位相をシフトさせながら前記撮像手段に複数枚の画像を取得させる制御手段と、前記照明手段の透過照明光が前記被観察物を透過することで変形された前記撮像手段で取得された前記被観察物の画像の明暗部の歪を補正するように画像処理する補正手段と、を有することを特徴とする。 Fifth monitoring apparatus invention comprises an illuminating means for transmission illumination the I Rikomu observation object surface light source formed by providing a light and dark portions are alternately emitted from the object under observation which is illuminated by said illuminating means An imaging optical system for imaging light, an imaging means for acquiring an image of the object formed by the imaging optical system, and a plurality of sheets on the imaging means while shifting the light / dark phase of the surface light source The control means for acquiring an image, and the distortion of the bright and dark part of the image of the observed object acquired by the imaging means deformed by the transmitted illumination light of the illuminating means being transmitted through the observed object. And a correction means for image processing .
第６の発明の観察装置は、第５の発明の観察装置において、前記補正手段は、前記照明手段の明暗部を直線にした状態で前記撮像手段により画像を取得し、取得された各画像の明暗部が直線になるように補正することを特徴とする。 Sixth monitoring apparatus of the present invention is directed to the observation device of the fifth invention, the correcting means, by the imaging means in a state where the light dark portion of the illumination means in a straight line to acquire an image of each image acquired bright dark part and correcting so that a straight line.
第７の発明の観察装置は、第６の発明の観察装置において、前記補正手段は、取得された画像の明暗部が直線になるように補正した後、前記被観察物のサイズを元に戻す補正を行うことを特徴とする。 Seventh monitoring apparatus of the present invention is directed to the observation apparatus of the sixth aspect of the invention, the correction means after the light dark portion of the acquired image is corrected to be a straight line, back to the original size of the object under observation Correction is performed.
Claims (8)
前記照明手段により照明された前記被観察物からの射出光を結像する結像光学系と、
前記結像光学系が形成した前記被観察物の光像を取得する撮像手段と、
前記照明手段の透過照明光が前記被観察物を透過することで変形された前記面光源の明暗部の光像を補正する照明光補正手段と、
前記照明光補正手段により補正された前記面光源の明暗部の位相をシフトさせながら前記撮像手段に複数の光像を取得し、前記複数の光像から合成画像を生成する画像合成手段と、
を有することを特徴とする観察装置。 Has a surface light source formed by providing alternating between bright and dark, and illuminating means for transmitting illuminating the O Rikomu observation object to the surface light source,
An imaging optical system that forms an image of light emitted from the object illuminated by the illumination means;
An imaging means for acquiring a light image of the observation object formed by the imaging optical system;
Illumination light correction means for correcting a light image of a bright and dark part of the surface light source deformed by transmission of the illumination light of the illumination means through the object to be observed;
Image synthesizing means for acquiring a plurality of light images in the imaging means while shifting the phase of the bright and dark portions of the surface light source corrected by the illumination light correcting means, and generating a synthesized image from the plurality of light images;
An observation apparatus comprising:
前記補正手段は、前記撮像手段で取得された光像の明暗部が直線になるように前記面光源の照明状態を補正することを特徴とする観察装置。 The observation apparatus according to claim 1,
Said correction means, an observation apparatus bright dark light image acquired by the imaging means and corrects the illumination state of the surface light source so that a straight line.
前記補正手段は、前記透明容器に関する情報に基づいて、前記面光源の照明状態を補正し、
前記透明容器に関する情報は、透明容器の種類、透明容器の材質、培養液の種類、培養液の量であることを特徴とする観察装置。 The observation apparatus according to claim 2,
The correction means corrects the illumination state of the surface light source based on information on the transparent container,
The information regarding the transparent container is the type of the transparent container, the material of the transparent container, the type of the culture solution, and the amount of the culture solution.
前記補正手段は、前記撮像手段で取得した前記被観察物の光像の画像から前記明暗部の形状変化を求め、前記撮像手段の画素ごとの歪補正率を求め、前記面光源の照明状態を補正し、
前記面光源は、透過型液晶パネルであることを特徴とする観察装置。 The observation apparatus according to claim 2 ,
The correction means obtains the shape change of the bright and dark part from the image of the optical image of the observation object acquired by the imaging means, obtains the distortion correction rate for each pixel of the imaging means, and determines the illumination state of the surface light source Correct ,
The observation apparatus , wherein the surface light source is a transmissive liquid crystal panel .
前記照明手段により照明された前記被観察物からの射出光を結像する結像光学系と、
前記結像光学系が形成した前記被観察物の画像を取得する撮像手段と、
前記面光源の明暗の位相をシフトさせながら前記撮像手段に複数枚の画像を取得させる制御手段と、
前記照明手段の透過照明光が前記被観察物を透過することで変形された前記撮像手段で取得された前記被観察物の画像の明暗部の歪を補正するように画像処理する補正手段と、
を有することを特徴とする観察装置。 Illuminating means for transmission illumination the I Rikomu observation object surface light source formed by providing alternating between bright and dark,
An imaging optical system that forms an image of light emitted from the object illuminated by the illumination means;
Imaging means for acquiring an image of the object to be observed formed by the imaging optical system;
Control means for causing the imaging means to acquire a plurality of images while shifting the light-dark phase of the surface light source;
Correction means for performing image processing so as to correct distortion of a bright and dark part of the image of the object acquired by the imaging means deformed by transmission of the transmitted illumination light of the illumination means through the object ;
An observation apparatus comprising:
前記補正手段は、前記照明手段の明暗部を直線にした状態で前記撮像手段により画像を取得し、取得された各画像の明暗部が直線になるように補正することを特徴とする観察装置。 The observation apparatus according to claim 5, wherein
It said correction means, observation apparatus obtains an image by the image pickup means while the bright dark portion in a straight line, light dark portion of each image acquired and correcting so that the straight line of the illumination means.
前記補正手段は、取得された画像の明暗部が直線になるように補正した後、前記被観察物のサイズを元に戻す補正を行うことを特徴とする観察装置。 The observation apparatus according to claim 6, wherein
Wherein the correction means, the observation light dark portion of the acquired image after correcting to a straight line, and performs a correction to restore the size of the object under observation device.
前記透明容器は、複数のウェルを有するウェルプレートであり、所定の１つのウェルを補正用の情報のみを取得するウェルとされていることを特徴とする観察装置。 The observation device according to any one of claims 1 and 4 to 7,
The transparent device is a well plate having a plurality of wells, and a predetermined one well is a well that acquires only correction information.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008202911A JP5104637B2 (en) | 2008-08-06 | 2008-08-06 | Observation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008202911A JP5104637B2 (en) | 2008-08-06 | 2008-08-06 | Observation device |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010039262A JP2010039262A (en) | 2010-02-18 |
| JP2010039262A5 true JP2010039262A5 (en) | 2012-03-29 |
| JP5104637B2 JP5104637B2 (en) | 2012-12-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2008202911A Expired - Fee Related JP5104637B2 (en) | 2008-08-06 | 2008-08-06 | Observation device |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013148856A (en) * | 2011-12-22 | 2013-08-01 | Canon Inc | Imaging device, display screen generation device, imaging system, and control method of the same |
| JP6527273B2 (en) | 2017-09-28 | 2019-06-05 | 株式会社片岡製作所 | Phase contrast observation device and cell processing device |
| CN111164487B (en) * | 2017-09-28 | 2022-04-15 | 株式会社片冈制作所 | Phase difference observation device and cell processing device |
| WO2019163369A1 (en) * | 2018-02-20 | 2019-08-29 | 富士フイルム株式会社 | Determination apparatus, cell detachment/collection apparatus, and determination method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4685569B2 (en) * | 2005-09-20 | 2011-05-18 | 高電工業株式会社 | Particle reaction pattern determination device |
| JP5091466B2 (en) * | 2005-11-30 | 2012-12-05 | 国立大学法人浜松医科大学 | Observation device |
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