JP2010032218A - Reaction treatment apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a reaction treatment apparatus capable of performing precise reaction treatment while preventing the penetration of foreign matter from the outside of a reaction container plate or the environmental pollution to the outside. <P>SOLUTION: The reaction treatment apparatus is equipped with a plate holding part 1 for holding the reaction container plate, a syringe driving part 2 for driving the syringe provided in the reaction container plate, a valve driving part 3 for driving a switching valve and a temperature regulating part 4 as a reaction treatment part for regulating the temperature of a reaction container to a predetermined temperature to perform reaction. The operations of the syringe driving part 2, the valve driving part 3 and the temperature regulating part 4 are controlled by a control part 5. The control part 5 performs control so as not only to connect the syringe to a sample container 35 before analyzing operation using the reaction reagent preliminarily housed in the sample container 35 is started but also to more reduce the pressure on the side of a liquid housing part 35g than that on the side of an air venting part 35i in the sample container 35 by driving the syringe toward a suction side. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a reaction processing apparatus for performing reaction processing using a reaction vessel plate suitable for performing various types of analysis and analysis in the field of medical analysis and chemistry in the fields of biological analysis, biochemical analysis, or chemical analysis in general. It is about.

  A micro multi-chamber apparatus is used as a small reaction apparatus used for biochemical analysis or normal chemical analysis. As such an apparatus, for example, a microwell reaction vessel plate such as a microtiter plate in which a plurality of wells are formed on a flat substrate surface is used (see, for example, Patent Document 1).

Moreover, as a trace liquid weighing structure capable of quantitatively handling a trace amount of liquid, a first channel and a second channel, a third channel opening in the channel wall of the first channel, A structure having a fourth channel having a property of opening the channel wall of the two channels and connecting one end of the third channel and the second channel so that capillary attraction is relatively difficult to work than the third channel. Some are provided (see, for example, Patent Documents 2 and 3). According to the trace liquid weighing structure, after the liquid introduced into the first flow path is drawn into the third flow path, the liquid remaining in the first flow path is removed, and the volume of the third flow path is increased. A corresponding volume of liquid can be weighed into the second flow path.
JP-A-2005-177749 JP 2004-163104 A JP 2005-114430 A Japanese Patent No. 3454717

When the conventional microwell reaction container plate is used, the upper surface of the reaction container plate is open to the atmosphere. Therefore, foreign matter may enter the sample from the outside, and conversely, the reaction product may contaminate the external environment.
Further, in the trace liquid weighing structure disclosed in Patent Documents 2 and 3, liquid introduction ports are formed at both ends of the first flow path and at both ends of the second flow path. It is possible that the reaction products are open and pollute the external environment through these ports.

  Therefore, the present inventors include a reaction vessel for causing a sample to react, a reaction vessel channel connected to the reaction vessel for circulating a sample, a reaction reagent, etc., and a sample, a reagent, etc. In addition to a sealing container, a sealing container flow path that can be connected to the sealing container, a switching valve for switching and connecting a reaction container flow path or a sealing container flow path to a syringe for sending a liquid or a syringe Etc. are integrated in one plate, and a reaction vessel plate using a reaction vessel, a reaction vessel channel, a sealed vessel, and a sealed vessel channel as a closed system has been proposed. According to the proposed reaction vessel plate, it is possible to prevent foreign substances from entering from the outside of the reaction vessel plate and environmental pollution to the outside.

  It is an object of the present invention to provide a reaction processing apparatus that can perform an accurate reaction process using the above-described reaction container plate while preventing entry of foreign matters from the outside of the reaction container plate and environmental pollution to the outside. It is what.

  The present invention relates to a reaction container, a reaction container flow path connected to the reaction container, a liquid container containing a liquid to be handled in the reaction process, and an air vent provided as a space at a position where the liquid container does not touch the liquid. And a reaction container via a switching valve, a sealing container channel connected to the liquid container of the sealing container, an air vent channel connected to the air vent of the sealing container, and a switching valve It is the reaction processing apparatus which processes the reaction container plate provided with the syringe connected to a flow path or a sealing container flow path. The reaction processing apparatus includes a plate holding unit for holding the reaction vessel plate, a valve driving unit for driving a switching valve of the reaction vessel plate, a syringe driving unit for driving a syringe, And a control unit for controlling the valve driving unit, the syringe driving unit, and the reaction processing unit. Then, the control unit is configured so that the sealing container channel is connected to the liquid container of the sealing container of the reaction container plate and the air vent channel is connected to the air vent of the sealing container. Before performing the process of using the liquid in the container, the syringe is connected to the sealed container flow path and the syringe is driven to the suction side, and the liquid containing part side is depressurized in the sealed container rather than the air vent part side. It controls so that the process to perform may be performed.

  The reaction vessel plate targeted by the reaction processing apparatus of the present invention includes a reaction vessel, a reaction vessel flow path, a sealed container in which a liquid to be handled in the reaction process is previously stored, a sealed vessel flow path, and a switching valve. It is equipped with a syringe connected to the reaction vessel channel or the sealed vessel channel, so that the reaction process can be performed using such a reaction vessel plate, a valve drive unit, a syringe drive unit, a reaction A processing unit and a control unit for controlling them are provided. The sample and the reaction reagent are fed to the reaction container by controlling the syringe and the switching valve, and the reaction process of the sample is performed in the reaction container by the reaction processing unit. The “reaction processing unit” has a function of adjusting the temperature of the reaction vessel to a temperature for causing a reaction between the sample and the reaction reagent.

  Further, the reaction container plate used in the reaction processing apparatus is attached to the sealing container in order to reduce the pressure change in the sealing container and smooth the flow of the liquid in the liquid container through the sealing container channel. Is provided with an air vent connected to the air vent channel. However, in this structure, a liquid such as a reaction reagent previously stored in the liquid storage part of the sealed container may enter the air vent part side due to vibration or impact when the reaction container plate is transported. . If this happens, the amount of liquid stored in the liquid storage portion will be less than a predetermined amount, and if the liquid is a reaction reagent, the amount of reaction reagent added to the sample will decrease and accurate reaction processing cannot be performed. . Therefore, the reaction processing apparatus of the present invention connects the syringe to the liquid container of the sealed container in which the liquid is sealed in advance before starting the analysis operation of sending the reaction reagent to the reaction container, and moves the syringe to the suction side. A step of depressurizing the liquid container side rather than the air vent part side by driving is performed.

  The reaction processing apparatus of the present invention includes a reaction vessel, a reaction vessel flow channel, a sealed vessel, a sealed vessel flow channel, a switching valve, and a syringe, and a sealed reaction vessel for circulating a sample and a reaction reagent in a closed system. A device for processing a plate, a valve driving unit for driving a switching valve of the reaction container plate, a syringe driving unit for driving a syringe, a reaction processing unit for performing a reaction process of a sample in the reaction container, and a control for controlling them Therefore, it is possible to automatically perform an analysis operation in which a sample or reaction reagent sealed in a sealed container is sent to the reaction container and a reaction process is performed in the reaction container. Furthermore, before starting the reaction processing operation using the liquid previously stored in the liquid storage part of the sealed container, the liquid that has entered the air venting part side can be returned to the liquid storage part side, and the reaction processing can be performed. It can be performed with high accuracy.

FIG. 1 is a block diagram schematically showing a reaction processing apparatus according to an embodiment.
In addition to the reaction vessel, the reaction vessel plate targeted by the reaction processing apparatus of this embodiment is a sealed container provided separately from the reaction vessel, and sucks liquid from the sealed container or puts the sucked liquid into another container. A single plate equipped with a syringe that can be injected, a flow path for circulating liquid between the syringe and the reaction container or the sealing container, a switching valve for switching the connection of the flow path, etc. It is.

  In order to handle the reaction container plate described above, the reaction processing apparatus includes a plate holding part 1 for holding the reaction container plate 7, a syringe driving part 2 for driving a syringe provided on the reaction container plate, and a switching valve. And a temperature adjusting unit 4 as a reaction processing unit for reacting by adjusting the temperature of the reaction vessel to a predetermined temperature. The operations of the syringe drive unit 2, the valve drive unit 3, and the temperature adjustment unit 4 are controlled by the control unit 5. The control unit 5 performs control based on the program held in the program holding unit 6. Specific operation of the reaction processing apparatus will be described later.

  2A and 2B are diagrams showing an example of a reaction vessel plate targeted by the reaction processing apparatus of the embodiment, where FIG. 2A is a plan view, and FIG. 2B is a measurement flow in a cross section at the position AA in FIG. It is the figure which added the cross section of the channel | path 15, the injection | throwing flow path 17, the reaction container air vent flow paths 19 and 21, the liquid drain space 29, the air drain space 31, and the bellows 53.

  A region in which a plurality of reaction vessels 10 are arranged with their upper surfaces sealed is provided. In the same region, a main channel 13, a metering channel 15, an injection channel 17, reaction vessel air vent channels 19, 21 and drain space air vent channels 23, 25 are also provided. The main channel 13, the metering channel 15 and the injection channel 17 constitute a reaction vessel channel. In FIG. 2A, each flow path that is not actually seen from above is shown for convenience.

  One end 13a of the main flow path 13 is connected to a connection port to a syringe connection flow path 63a of a switching valve 63 described later. The other end of the main channel 13 is connected to the liquid drain space 29. One end of a drain space air vent channel 23 is connected to the liquid drain space 29. The other end 23 a of the drain space air vent channel 23 is connected to a connection port of the switching valve 63 to the syringe connection channel 63 a.

  The metering channel 15 is a channel branched from the main channel 13 for each reaction vessel 10 and is connected to each reaction vessel 10 via an injection channel 17. The channel width of the branching portion of the metering channel 15 from the main channel 13 is wider than the channel width of the main channel 13 immediately downstream thereof, and the channel resistance is small. 15 flows preferentially into the flow channel 15 and flows downstream of the main flow channel 13 after filling the metering flow channel 15.

  The injection channel 17 is formed with a dimension that maintains liquid tightness in the reaction vessel 10 in a state where there is no pressure difference between the reaction vessel 10 and the injection channel 17. The injection channel 17 is composed of a plurality of grooves.

  The reaction container air vent channel 21 is connected to each reaction container 10 via the reaction container air vent channel 19 and one end is connected to the air drain space 31. The reaction vessel air vent channel 19 has a size and a structure that keeps liquid tightness in the reaction vessel 10 in a state where there is no pressure difference between the reaction vessel 10 and the reaction vessel air vent channel 19. It will be. One end of a drain space air vent channel 25 is connected to the air drain space 31. The other end 25 a of the drain space air vent channel 25 is connected to a connection port to the arc-shaped channel 63 b of the switching valve 63.

  A sample container 35, a reagent container 37, and an air suction container 39 are provided as sealing containers in a region different from the region where the reaction containers 10 are arranged. Although not shown in FIG. 2B (only the sample container 35 is shown), the upper surfaces of these containers 35, 37 and 39 are sealed, and in particular, the upper surface of the sample container 35 is an elastic member. It is sealed with a sealing member including a septum. Hereinafter, these containers 35, 37 and 39 will be described with reference to FIG.

  The sample container 35 is accommodated in the sample container accommodating portion 36. A sample channel 35 a and a sample container air vent channel 35 b are provided below the sample storage unit 35. One end of the sample flow path 35a is a first protruding flow path 35d protruding upward from the bottom of the sample container housing portion 36, and one end of the sample container air vent flow path 35b is a second protruding flow path 35e protruding upward. . As shown in FIG. 2A, the other end of the sample container channel 35a serves as a connection port to the syringe connection channel 63a of the switching valve 63, and the other end of the sample container air vent channel 35b. Is a connection port to the arc-shaped flow path 63b of the switching valve 63. An annular packing 35f such as an O-ring is provided on the outer peripheral side surface of the base end portion of the first projecting portion 35d and the outer peripheral side surface of the base end portion of the second projecting portion 35e.

  The sample container 35 includes a liquid container 35g for storing a sample solution and a reagent, and an air vent 35i provided as a protruding portion on the upper side surface of the liquid container 35g. The sample storage part 35g and the sample container air vent part 35i are communicated with each other by a flow path 35h provided so as to be above the liquid level of the liquid stored in the liquid storage part 35g.

  The upper surface of the sample container 35 is sealed with, for example, a film 35k made of aluminum and a septum 41 made of an elastic member, and a liquid such as a sample can be injected by piercing a dispensing device such as a syringe. The septum 41 can elastically close the hole after the dispensing device is pulled out. The flow path 35h is formed so as to maintain the liquid tightness of the liquid storage portion 35g in a state where there is no pressure difference between the liquid storage portion 35g and the sample container air vent portion 35i. In this example, the reagent 45 is previously stored in the liquid storage portion 35g.

  The bottom surface of the sample container 35g of the sample container 35 is a first through portion 35j that can be penetrated by the protruding flow path 35a, and the bottom surface of the sample container air vent 35i is the second through portion 35l that can be penetrated by the protruding flow path 35e. It has become. The through portions 35j and 35l may be made of, for example, a film made of aluminum, or may be formed together with the sample container 35 by integral molding.

  A locking claw 35c for holding the sample container 35 is provided in the vicinity of the sample container housing portion 36, and locking grooves 35m and 35n for engaging with the locking claw 35c are provided on the outer peripheral surface of the sample container 35. It has been. When not in use, the sample container 35 is held with the bottom surface of the sample container 35 slightly lifted from the bottom of the sample container housing portion 36, and the sample container 35 is kept until the bottom surface of the sample container 35 reaches the bottom of the sample container housing portion 36. It can be used by pushing down. In a state where the bottom surface of the sample container 35 is slightly lifted from the bottom of the sample container housing portion 36, the first through portion 35j and the first protrusion portion 35d, and the second through portion 35l and the second protrusion portion 35e face each other (see FIG. 3 (E).) By pushing the sample container 35 downward from this state, the first protrusion 35d passes through the first penetration part 35j, the second protrusion 35e passes through the penetration part 35l, and the sample storage part 35g and the sample container flow path 35a. The sample container air vent part 35i and the sample container air vent channel 35b are connected to each other (see FIG. 3F). The lower surface of the sample container main body of the sample container 35 is pressed against the packing 35f, thereby preventing liquid leakage and air leakage.

  The reagent container 37 and the air suction container 39 have the same structure as the sample container 35, and each includes a liquid storage part (the air suction container 39 does not store a liquid) and an air vent part. A reagent container channel 37a, a reagent container air vent channel 37b, an air suction container channel 39a, and an air suction container air vent channel 39b, each of which is connected at one end, are a sample container channel 35a and a sample container air. It is provided in the same manner as the extraction channel 35b.

Returning to FIG. 2, the description of the reaction vessel plate will be continued.
The syringe 51 includes a cylinder 51a having an open upper surface, a plunger 51b disposed in the cylinder 51a so as to be slidable in the vertical direction, and a cover body 51d. One end of a syringe channel 51c communicates with the bottom of the cylinder 51a. The other end of the syringe channel 51 c is connected to a port provided at the rotation center of the switching valve 63.

  The cover body 51d is flexible in the sliding direction of the plunger 51b, and one end is connected to the cylinder 51a and the other end is connected to the plunger 51b. A space surrounded by the cylinder 51a, the plunger 51b, and the cover body 51d is connected to the bellows 53 via a syringe air vent channel 53b. The bellows 53 expands and contracts to passively change the sealed internal capacitance. Thereby, the plunger 51b slides smoothly. One end of a bellows flow path 53 a is connected to the bellows 53, and the other end of the bellows flow path 53 a is a port for connecting to an arcuate flow path 63 b in the switching valve 63.

  The switching valve 63 is provided at a position below the syringe 51 on the lower surface side of the reaction container plate. The switching valve 63 is a rotary type switching valve that switches the connection destination of the syringe connection flow path 63a by rotating a rotor including a syringe connection flow path 63a extending in the radial direction from the center. One end of the syringe connection channel 63a is directly connected to the port at the center of the valve, and the syringe channel 51c is connected to the center port. The other end of the syringe connection channel 63a is a connection port to the syringe channel 51c, and the syringe channel 51c can be switched to one of the channels 13, 35a, 37a, and 39a by rotating the rotor. it can. Further, the rotor of the switching valve 63 also includes an arc-shaped channel 63b, and the bellows channel 53a can be switched and connected to any one of the channels 23a, 25a, 35b, 37b, and 39b. FIG. 2A shows an initial state where the syringe flow path 51c and the bellows flow path 53a are not connected to any flow path.

4 is a cross-sectional view specifically showing the reaction processing apparatus shown in FIG. In addition, illustration of the plate holding part 1 is abbreviate | omitted in this figure.
The syringe drive unit 2 is disposed above the reaction container plate, and can move the plunger 51b of the syringe 51 in an up and down direction by an arbitrary distance, for example, by driving a stepping motor or the like.
The valve drive unit 3 is disposed below the rotor of the switching valve 63 when the switching valve 63 is switched, and can be rotated by an arbitrary angle in the in-plane direction.
The temperature adjustment unit 4 is configured by, for example, a Peltier element, and is disposed immediately below the region where the reaction vessels 10 are arranged, and can adjust the temperature of the reaction vessel 10 to a predetermined temperature.

  As described above, the operations of the syringe drive unit 2, the valve drive unit 3, and the temperature adjustment unit 4 are configured to be controlled by the control unit 5 based on a program held in the program holding unit 6. FIG. 5 is a flowchart showing an example of the reaction processing operation of the reaction processing apparatus of the embodiment. The operation during the reaction process will be described below with reference to FIGS. 6 to 12 together with this flowchart.

  Before the reaction processing apparatus starts an analysis operation, for example, 5 μL of sample liquid is injected into the sample container 35 through the septum 41 using a dispensing device such as a syringe as preprocessing by the analyst. This operation may be performed before setting the reaction container plate in the plate holding part of the reaction processing apparatus, or may be performed after setting the reaction container plate in the plate holding part. Further, the sample container 35, the reagent container 37, and the air suction container 39 are pushed downward, and the liquid storage part of each container 35, 37, 39 and the bottom part of the air vent part are penetrated by the protruding flow path. The flow path corresponding to each part of 37 and 39 is connected. Note that the sample liquid can be injected into the sample container 35 after the pushing operation.

  After the preprocessing by the analyst, the processing operation by the reaction processing apparatus is started. The switching valve 63 is switched by the valve drive unit 3, the syringe channel 51c is connected to the sample channel 35a, and the bellows channel 53a is connected to the sample container air vent channel 35b (step S1) (see FIG. 6). At this time, the air vent channels 37b and 39b are also connected to the bellows channel 53a.

  The syringe 51 is driven to the suction side by the syringe drive unit 2, and the internal pressure on the liquid container 35g side of the sample container 35 is reduced from the air vent 35i side (step S2). For example, 45 μL of the reagent 45 is stored in advance in the liquid storage portion 35g of the sample container 35. However, the reagent 45 enters the air vent portion 35i side due to vibration or impact of the reaction container plate. In some cases, the negative pressure is applied to the liquid container 35g side to return the reagent 45 entering the air vent 35≡ side to the liquid container 35g. Further, the sample container 35 is agitated by reciprocating the syringe 51 a plurality of times in the suction direction and the discharge direction to mix the sample liquid and the reagent 45 (step S3). Thereafter, the mixed solution is sucked by, for example, 10 μL with the syringe 51 (step S4).

Next, the switching valve 63 is switched by the valve drive unit 3, the syringe flow path 51c is connected to the reagent flow path 37a, and the bellows flow path 53a is connected to the reagent container air vent flow path 37b (step S5) (FIG. 7). reference.). For example, 190 μL of dilution water 49 is stored in the reagent container 37 in advance. The syringe 51 is driven to the discharge side by the syringe drive unit 2, and the mixed solution sucked by the syringe 51 is injected into the reagent container 37 (step S6). Furthermore, the mixture 51 and the dilution water 49 are mixed by agitating the inside of the reagent container 37 by reciprocatingly driving the syringe 51 in the suction direction and the discharge direction (step S7). After mixing, by driving the syringe 51 in the suction direction, 200 μL (all) of the diluted mixed solution is sucked (step S8).
In addition, before injecting the mixed liquid into the reagent container 37 (between steps S5 and S6), the syringe 51 is further driven to the suction side, and the internal pressure on the liquid storage part side in the reagent container 37 is reduced more than that on the air vent part side. It is also possible to add the step of performing the process so that the dilution water 49 entering the air vent channel side is returned to the liquid container.

  The switching valve 63 is switched by the valve drive unit 3 so that the syringe flow path 51c is connected to one end 13a of the main flow path 13 and the bellows flow path 53a is connected to one ends 23a and 25a of the drain space air vent flow paths 23 and 25 ( Step S9) (see FIG. 8). The syringe 51 is driven in the discharge direction by the syringe drive unit 3, and the diluted mixed solution sucked in the previous step is injected into the main flow path 13 (step S10). The diluted mixed solution injected into the main channel 13 fills the metering channel 15 in order from the upstream side and reaches the liquid drain space 29.

  The switching valve 63 is switched by the valve drive unit 3, the syringe flow path 51c is connected to the air suction flow path 39a, and the bellows flow path 53a is connected to the air suction container air vent flow path 39b (Step S11). 9). The syringe drive unit 2 drives the syringe 51 to the suction side to suck the gas in the air suction container 39 (step S12).

  The switching valve 63 is switched by the valve drive unit 3 so that the syringe flow path 51c is connected to one end 13a of the main flow path 13 and the bellows flow path 53a is connected to one ends 23a and 25a of the drain space air vent flow paths 23 and 25 ( Step S13) (see FIG. 10). This state is the same as the connection state of FIG. The syringe 51 is driven in the discharge direction by the syringe drive unit 2, and the gas sucked in the previous step is sent to the main channel 13 to purge the diluted mixed solution in the main channel 13 (step S14) (see the white arrow in FIG. 10). ). In the purge pressure state at this time, the diluted mixed solution does not pass through the injection flow channel 17 and remains in the measurement flow channel 15 (see embossing). The purged diluted liquid mixture is accommodated in the liquid drain space 29.

  The switching valve 63 is switched by the valve drive unit 3, the syringe flow path 51c is connected to the air suction flow path 39a, and the bellows flow path 53a is connected to the air suction container air vent flow path 39b (Step S15). 11). This state is the same as the connection state of FIG. The syringe 51 is driven to the suction side by the syringe drive unit 2, and the gas in the air suction container 39 is sucked (step S16).

  The switching valve 63 is switched by the valve drive unit 3, the syringe flow path 51c is connected to one end 13a of the main flow path 13, and the bellows flow path 53a is connected to one end 25a of the drain space air vent flow path 25 (step S17) ( See FIG. This state differs from the connected state shown in FIGS. 8 and 10 in that one end 23a of the drain space air vent channel 23 is not connected to the bellows channel 53a. Since one end 23a of the drain space air vent channel 23 is not connected to the bellows channel 53a, the downstream end of the main channel 13 is closed. In this state, the syringe drive unit 2 drives the syringe 51 in the discharge direction. The inside of the main channel 13 is pressurized higher than the liquid introduction pressure and the purge introduction pressure, and the diluted mixed solution in the metering channel 15 passes through the injection channel 17 and is injected into the reaction vessel 10 (step S18).

  Thereafter, the switching valve 63 is switched by the valve driving unit 3 so as to be in the connection state of FIG. 2, thereby sealing the container, the flow path, and the drain space inside the reaction container plate (step S <b> 19). In this state, the reaction vessel 10 is heated to a predetermined temperature by the temperature adjusting unit 4 to melt the wax 12 (step S20). The diluted mixed solution injected into the reaction vessel 10 enters under the wax 12, and the diluted mixed solution and the reagent 11 are mixed and reacted.

  In addition, the wax 12 may be melted by heating the reaction vessel 10 by the temperature adjusting unit 4 before injecting the diluted mixed solution into the reaction vessel 10. In that case, the diluted mixed solution injected into the reaction vessel 10 immediately enters under the wax 12, and the diluted mixed solution and the reagent 11 are mixed and reacted. Even if the flow path connection state by the switching valve 63 is the state of FIG. 12, the sealed system is secured by the bellows 53. If the switching valve 63 is brought into the connection state shown in FIG. 2 after the diluted mixed solution is injected, the container, flow path and drain space inside the reaction container plate can be sealed. Here, the timing of switching the switching valve 63 to the connected state of FIG. 2 may be any timing from immediately after the injection of the diluted mixed solution to the end of the reaction of the diluted mixed solution and the reagent 11, or the diluted mixed solution and the reagent 11. It may be after the completion of the reaction.

  In the above embodiment, when the liquid filled in the metering channel 15 is injected into the reaction vessel 10 through the injection channel 17, the inside of the main channel 13 after air purge is pressurized to supply the liquid to the reaction vessel 10. However, the reaction treatment method of the present invention is not limited to this. For example, by changing the flow channel configuration so that the inside of the reaction vessel air vent channel 21 can be set to a negative pressure using the syringe 51, and by setting the inside of the reaction vessel air vent channel 21 and thus the inside of the reaction vessel 10 to a negative pressure. The liquid filled in the metering channel 15 may be injected into the reaction vessel 10 through the injection channel 17. Alternatively, a separate syringe may be prepared so that the liquid is injected into the reaction vessel 10 with a positive pressure in the main channel 13 and a negative pressure in the reaction vessel 10.

It is a block diagram which shows roughly the reaction processing apparatus of one Example. It is a figure which shows one Example of reaction container plate, (A) is a schematic top view, (B) is a cross section in the AA position of (A), a bellows, drain space, a measurement flow path, an injection flow path FIG. 3 is a schematic cross-sectional view with a cross section of a sample container air vent channel added. It is the figure which expanded and showed the sample container accommodating part and sample container of the Example, (A) is a top view of a sample container accommodating part, (B) is sectional drawing in the BB position of (A), (C) is a plan view of the sample container, (D) is a cross-sectional view at the C-C position in (C), (E) is a cross-sectional view in which the sample container is arranged in the sample container accommodating portion at the first holding position, (F) ) Is a cross-sectional view in which the sample container is disposed in the sample container housing portion at the second holding position. It is sectional drawing which showed the reaction processing apparatus concretely. It is a flowchart which shows roughly an example of the operation | movement at the time of the reaction process of a reaction processing apparatus. It is a top view which shows one process for demonstrating an example of the operation | movement at the time of the reaction process of a reaction processing apparatus. It is a top view for demonstrating the operation | movement following FIG. It is a top view for demonstrating the operation | movement following FIG. It is a top view for demonstrating the operation | movement following FIG. FIG. 10 is a plan view for explaining the operation following FIG. 9. It is a top view for demonstrating the operation | movement following FIG. It is a top view for demonstrating the operation | movement following FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Plate holding part 2 Syringe drive part 3 Valve drive part 4 Temperature control part 5 Control part 6 Program holding part 7 Reaction container plate 10 Reaction container 13 Main flow path 15 Metering flow path 17 Injection flow path 19, 21 Reaction container air vent flow path 35 Sample container 35a Sample container flow path 35b Sample container air vent flow path 35c Locking claw 35d, 35e Protruding portion 35f Packing 35j, 35l Through hole 35m, 35n Locking groove 37 Reagent container 37a Reagent container flow path 37b Reagent container air Vent channel 39 Air suction container 39a Air suction container channel 39b Air suction container air vent channel 41 Septum 51 Syringe 51a Cylinder 51b Plunger 51d Cover body 53 Bellows 53a Bellows channel 53b Syringe air vent channel 63 Switching valve

Claims (1)

A seal provided with a reaction vessel, a reaction vessel flow path connected to the reaction vessel, a liquid containing portion containing a liquid to be handled in a reaction process, and an air vent provided as a space at a position where the liquid is not touched A stop container, a sealed container channel connected to the liquid container of the sealed container, an air vent channel connected to the air vent of the sealed container, and the reaction container channel or A reaction processing apparatus for processing a reaction container plate provided with a syringe connected to the sealing container flow path,
A plate holder for holding the reaction vessel plate;
A valve drive unit for driving the switching valve of the reaction vessel plate;
A syringe drive unit for driving the syringe;
A reaction processing unit for performing reaction processing of the sample in the reaction vessel;
A control unit for controlling the valve driving unit, the syringe driving unit, and the reaction processing unit,
In the state where the sealing container channel is connected to the liquid container of the sealing container of the reaction container plate and the air vent channel is connected to the air vent part of the sealing container, the control unit Before performing the step of using the liquid in the container, the syringe is connected to the sealing container flow path, and the syringe is driven to the suction side, and the liquid container is located in the sealing container more than the air vent part side. The reaction processing apparatus which controls to perform the process of decompressing the side.

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