JP2010020075A - Microscope system and microscope control program - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microscope system that optimally and easily sets a condition for each of the control sections of a microscope according to the characteristic of a target sample, and also achieves a method of operating the microscope. <P>SOLUTION: A storage device of a controller 11 stores guide information and condition information in association with each other, the guide information being used to indicate the contents of the operation for setting to obtain an image of a sample 10 into the microscope and the condition information being set for the microscope. The controller 11 guides the operation by displaying an operation guide display screen onto a display section 12 according to operation order, the operation guide display screen being used for showing the contents of the guide information on the storage device. The controller also displays a screen which shows the contents of the condition information associated with the guide information about the operation guide display screen being displayed. In this case, the controller 11 obtains instructions for the condition information and updates the condition information relating to the instruction and stored in the storage device. The controller sets the microscope according to the condition information stored in the storage device. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a microscope technique, and more particularly to a technique for supporting various conditions setting operations performed on a microscope for observation of a target sample and image acquisition.

In order to observe a target sample and acquire an image using a microscope, an operation of setting various conditions (operation conditions) in each control unit of the microscope is performed.
As a technology for supporting this work, a technology is known in which various conditions to be set are stored in advance as an observation condition file alone or integrated into an image file. In this technique, when a microscope is used, setting condition data is read from the observation condition file or image file, and conditions are set in each control unit according to the data.

  Also, when performing image processing and measurement in a predetermined procedure, the parameters of the image processing are registered in advance as a measurement condition file (recipe file), and the apparatus automatically executes a series of procedures according to the registered contents. Technology is also known.

  On the other hand, there is already a technology that allows users who are unfamiliar with a microscope to easily acquire images by presenting the procedure for setting conditions for each control unit of the microscope in a so-called wizard format that answers questions interactively. Proposed.

Furthermore, for example, Patent Document 1 discloses a technique of displaying an image construction procedure of a three-dimensional image on a guiding unit. According to Patent Document 1, this technique allows a user to manually operate the apparatus according to the guidance displayed on the guidance unit, so that procedures necessary for image acquisition can be performed without excess or deficiency, and thus unfamiliar with the operation of the apparatus. Even a simple user can easily observe and acquire a three-dimensional image.
JP 2006-49155 A

  In the existing technology, when an image is acquired in the past under a certain condition (for example, when the sample to be observed is the same or when there is no change in the surface state or height of the sample) It is effective in the case of image acquisition and observation of an equivalent sample. This is because it is possible to perform image acquisition and observation by reflecting, in the control unit of the microscope, the conditions at the time of image acquisition and observation of a similar sample that have been previously stored. However, for example, when a completely different sample is observed or when a partially different sample is observed, it is not always appropriate to apply the already stored conditions.

  However, even if all of these conditions are reset, it is very difficult to obtain the same result as that set by a skilled person without sufficient understanding of the apparatus.

  Furthermore, in the inspection process, an administrator (an operator who is familiar with the device) creates a work procedure manual that describes the conditions and operation procedures for image acquisition and observation, and the general operator (unfamiliar with the device) Instructions are included). A general operator can obtain an optimum image corresponding to a sample even if he / she is not accustomed to the apparatus by acquiring an image according to the work procedure manual. However, a general operator has to set conditions by alternately comparing the setting screen of the apparatus and the work procedure manual.

  In this way, especially for users who are not familiar with microscope operation, the condition setting operation for each control unit of the microscope is not easy, and there is a problem that a lot of work time is required by repeating trial and error. In addition, there is a problem that the image quality and accuracy of the acquired sample image depend on the skill level of the user of the microscope.

  The present invention has been made in view of the above-described problems, and the problem to be solved is that even if a user is unfamiliar with a microscope, the condition setting operation for each control unit is optimally performed according to the characteristics of the target sample. It is an object of the present invention to provide a microscope system that can be easily performed and can also learn a method of operating a microscope.

  A microscope system according to one aspect of the present invention includes guidance information, which is information indicating the contents of an operation for performing settings when acquiring an image of a target sample in a microscope, and information on conditions set for the microscope. A storage unit that stores certain condition information in association with each other, and an operation guide display screen that represents the content of the guide information stored in the storage unit is displayed according to the order of the operation to guide the operation. Condition information display for displaying an operation guidance unit to be performed and a screen representing the content of the condition information stored in the storage unit in association with guidance information on the operation guidance display screen displayed by the operation guidance unit A condition information instruction acquisition unit that acquires an instruction of the condition information, updates the condition information stored in the storage unit with the condition information related to the instruction, controls the microscope, and stores the storage unit Remembered The settings in accordance with the matter information and a control unit which performs relative to the microscope.

In the microscope system described above, the storage unit can be configured to store the condition information at the time of acquisition of images acquired in the past by the microscope.
At this time, from the image data file for the image acquired in the past by the microscope, or from the report data file containing the observation report data for the image, the conditions at the time of acquiring the image A sample condition information acquisition unit for acquiring information may be further included, and the storage unit may be configured to store the condition information acquired by the sample condition information acquisition unit.

  Further, in the microscope system according to the present invention described above, the condition information instruction acquisition unit is configured to operate the operation guide based on the operation guide display screen displayed by the operation guide unit when the condition information instruction is acquired. The condition information associated with the guidance information related to the display screen and stored in the storage unit can be updated to the condition information related to the acquired instruction.

  A microscope control program according to another aspect of the present invention is a program for causing a computer to control a microscope, and details of an operation for performing settings when acquiring an image of a target sample on the microscope. Storage processing for associating guidance information, which is information indicating information, and condition information, which is information on conditions set for the microscope, in the storage unit, and contents of guidance information stored in the storage unit Is displayed on the display unit in accordance with the order of the operations to guide the operation, and is associated with the guidance information related to the operation guide display screen displayed by the operation guidance processing. A condition information display process for causing the display unit to display a screen representing the contents of the condition information stored in the storage unit, and obtaining an instruction for the condition information, Condition information instruction acquisition processing for updating the condition information stored in the storage unit with information, and setting the microscope according to the condition information stored in the storage unit by controlling the microscope Control processing is performed by the computer.

  According to the present invention, even a user who is unfamiliar with a microscope can perform condition setting work for each control unit optimally and easily according to the characteristics of the target sample, and also learn how to operate the microscope. The effect is to be able to provide a simple microscope system.

Hereinafter, embodiments of the present invention will be described with reference to the drawings.
First, FIG. 1 will be described. FIG. 1 shows the configuration of a microscope system that implements the present invention. Although the configuration of this microscope system is that of a confocal scanning laser microscope system, the present invention is not limited to the implementation with this configuration, for example, by a disk scan using a white light source. The present invention can also be implemented in a confocal microscope or a microscope system having no confocal optical system.

The microscope system shown in FIG. 1 mainly includes a microscope main body 14, a controller 11, and a display unit 12.
The controller 11 is a standard computer and includes an MPU (arithmetic processing unit), a main memory, an interface device, and a storage device, which are not shown in FIG.

  Here, the MPU performs operation control of the entire microscope system of FIG. 1 by executing a control program stored in a storage device, for example. The main memory is a semiconductor memory used as a work memory by the MPU as needed. The interface device manages the exchange of various data with each component of the confocal scanning laser microscope system. The storage device is, for example, a hard disk device, and stores various control programs and data for realizing the control of the microscope system by the controller 11. Various control operations performed by the controller 11, which will be described later, are stored in advance in the storage device for a microscope system control program for causing the controller 11 to perform the control operation, and the MPU reads and executes the control program. This is realized.

  The controller 11 is connected to a pointing device such as a mouse device and an input device such as a keyboard device, but is not shown in FIG. A display unit 12 is connected to the controller 11. The display unit 12 is a display device, for example, and displays an operation screen, an observation image, and the like under the control of the MPU.

  In the microscope system shown in FIG. 1, a 1/4 λ plate 4, an objective lens 5, an electric XY stage 9, a Z revolver 13, a microscope main body 14, and a revolver 15 constitute a microscope that acquires an image of the sample 10. .

  In the configuration of FIG. 1, light (laser light) emitted from the laser light source 1 passes through a PBS (Polarization Beam Splitter) 2 and then is guided to the two-dimensional scanning unit 3. The two-dimensional scanning unit 3 scans the light beam two-dimensionally and guides it to the objective lens 5. Since the two-dimensional scanning unit 3 is arranged at a position conjugate with the pupil of the objective lens 5, the light beam incident on the pupil of the objective lens 5 becomes convergent light and scans on the focal plane of the sample 10. At this time, when the light beam passes through the ¼λ plate 4, the polarization characteristic is converted from linearly polarized light to circularly polarized light.

  This light is reflected from the surface of the sample 10. The reflected light at this time is again guided to the PBS 2 from the objective lens 5 through the ¼λ plate 4 and the two-dimensional scanning unit 3. When the light beam at this time passes through the ¼λ plate 4, the polarization characteristic is converted into linearly polarized light in a direction orthogonal to the light beam emitted from the laser light source 1, so that it is now reflected by the PBS 2. After that, the light is condensed at the position of the pinhole 7 by the imaging lens 6.

The microscope system can provide an optical zoom function by adjusting the scanning amplitude of the two-dimensional scanning unit 3.
The reflected light collected by the imaging lens 6 is cut by the pinhole 7 from the sample 10 other than the focal point of the sample 10, and only the reflected light from the focal point of the sample 10 passes through the pinhole 7. The light is received by the photodetector 8 and detected. The photodetector 8 outputs a signal having a magnitude corresponding to the brightness (luminance) of the light received at this time, and this output is guided to the controller 11.

  Note that the revolver 15 on which the objective lens 5 is mounted is movable in the optical axis direction (Z direction) by driving the Z revolver 13. Note that the optical scanning operation by the two-dimensional scanning unit 3 and the driving operation of the Z revolver 13 are controlled by the controller 11.

The revolver 15 can be equipped with a plurality of objective lenses 5, and the controller 11 can control selection of the objective lens 5 to be arranged on the optical axis.
The electric XY stage 9 can move the sample 10 placed in the XY direction (two directions on a plane perpendicular to the Z direction), and the controller 11 can control this movement operation.

  Here, the condensing position by the objective lens 5 is arranged so as to be optically conjugate with the position of the pinhole 7. Therefore, when the sample 10 is at a position where light is collected by the objective lens 5, the reflected light from the sample 10 can be condensed and pass through the pinhole 7. On the other hand, when the sample 10 is at a position deviated from the condensing position by the objective lens 5, the reflected light from the sample 10 does not condense at the position of the pinhole 7 and cannot pass through the pinhole 7.

  Therefore, the relationship between the relative position of the objective lens 5 and the sample 10 at this time and the output of the photodetector 8 is as follows. That is, when the sample 10 is at the condensing position of the objective lens 5, the output of the photodetector 8 is maximized. Then, as the relative position between the objective lens 5 and the sample 10 increases from this position, the output of the photodetector 8 rapidly decreases. Here, the two-dimensional scanning unit 3 is controlled to two-dimensionally scan the focal point on the surface of the sample 10, and the output of the photodetector 8 is imaged in synchronization with the optical scanning operation of the two-dimensional scanning unit 3. To do. Then, the image obtained at this time is imaged only at a specific height of the sample 10 and becomes an image (confocal image) as if the sample 10 was optically sliced.

  Further, it is assumed that the cross section of the sample 10 has a shape as shown in FIG. At this time, a plurality of confocal images (confocal slice images) are acquired while sequentially moving the objective lens 5 in the Z direction at a predetermined driving pitch in the range from Z1 to Z2 in FIG. The value that maximizes the output is extracted and set as the luminance value of the pixel. By doing so, an omnifocal image, which is an image focused on the entire surface of the sample 10, can be obtained.

  Next, a user interface provided by the microscope system of FIG. 1 will be described with reference to FIG. FIG. 3 shows a screen example of a user interface screen that the controller 11 displays on the display unit 12.

  First, when a microscope system control program installed in the controller 11 and stored in advance in a storage device provided in the controller 11 is activated, a screen shown in FIG. 3 is displayed on the display unit 12. This screen includes an operation guidance screen 301 and an observation image display unit 306.

In the screen example of FIG. 3, the operation guidance screen 301 includes an objective lens adjustment unit 302, an optical zoom control unit 303, an operation guidance unit 304, and a next process instruction unit 305.
The objective lens adjustment unit 302 is a screen used for an instruction to select the objective lens 5 arranged on the optical axis, and the optical zoom control unit 303 is used for an instruction to set the zoom magnification in the optical zoom function described above. It is a screen. These screens on the operation guide screen 301 are appropriately switched according to a change in a setting target for which a condition is set by the operation guide.

  The operation guiding unit 304 switches and displays setting objects for setting conditions in each procedure of operations performed on the microscope, setting methods, and setting contents according to the operation procedures, and guides the operation to the user. It is a screen to perform.

  The next process instruction unit 305 includes a “next” button, which is an instruction button used for giving an instruction to move to the next operation procedure after the operation of the procedure displayed on the operation guide unit 304 is completed, A “return” button that is an instruction button for giving an instruction to return to the operation procedure is provided.

The observation image display unit 306 displays an image of the sample 10 that is the observation target sample.
Next, FIG. 4 will be described. FIG. 4 is a flowchart showing the processing contents of the first example of the condition information acquisition processing. This process is a process in which condition information at the time of acquiring a microscope image acquired in the past is acquired from an image data file of the microscope image as a model in operation guidance and stored in a storage device.

This process is immediately started by the controller 11 by starting the above-described microscope system control program.
First, in S101, processing for displaying the user interface screen shown in FIG. 3 on the display unit 12 is performed.

  In S102, processing for acquiring an instruction of an image data file of an image suitable for acquisition of condition information (for example, an image acquired in the past by an expert of the microscope system in FIG. 1 operating this system) is performed. In this process, first, detection processing of a predetermined operation guidance information creation instruction performed by the user is performed on the input device. When the instruction is detected, an image data file such as the screen example shown in FIG. Display processing of the selection instruction screen on the display unit 12 is performed. Then, detection processing of a predetermined image file selection operation performed by the user on the input device is performed, and when the instruction is detected, processing for deleting the display of the screen of FIG. Processing for referring to the image file according to the instruction is performed.

In S103, processing for extracting one piece of condition information stored in the header portion of the image data file being referred to by the processing in S102 is performed.
An example of the condition information included in the header portion of the image data file for the image acquired by the microscope system of FIG. 1 is shown in FIG.

  In the example shown in FIG. 6, the various conditions set in the microscope at the time of image acquisition, the contents of image processing applied to the image, the contents of image processing parameters, and the processing order of image processing are as follows. , Shown as condition information.

  In the present embodiment, it is assumed that the condition information includes two types of information: microscope control condition information and operation condition information. Here, the microscope control condition information refers to conditions set in the microscope at the time of image acquisition, such as the magnification of the objective lens 5, the magnification of the optical zoom, the image size, the brightness setting value, and the like. In addition, the operation condition information refers to information (for example, information such as an execution procedure and parameters set at the time of execution) regarding operation processing such as image processing and image measurement performed on the acquired image.

  Returning to the description of the processing in FIG. In S104, a process for storing the condition information acquired in the process of S103 in a storage device in association with a setting item in an operation guidance process described later is performed.

  In S105, processing is performed to determine whether or not all the condition information stored in the header portion of the image data file being referred to has been extracted. If it is determined that all the condition information has been extracted (when the determination result is Yes), the process proceeds to S106. On the other hand, when it is determined that unextracted condition information remains (when the determination result is No), the process returns to S103, and the processes after S103 are performed on the unextracted condition information.

In S106, a process of shifting the operation state of the controller 11 to the operation guidance start state is performed, and thereafter the process of FIG. 4 is terminated.
The above processing is the first example of the condition information acquisition processing. When this process ends, the controller 11 that is executing the above-described microscope system control program subsequently starts the operation guidance process. The operation guidance process is a process for displaying a screen representing the procedure of an operation performed on the microscope to acquire the image of the sample 10 on the operation guidance unit 304 and guiding the operation to the user. . In addition, processing for setting the various conditions according to the operation performed by the user according to the guidance, for example, switching the objective lens 5, adjusting the focusing, driving the electric XY stage 9, etc. is also performed on the microscope. Done.

Next, FIG. 7 will be described. FIG. 7 is a flowchart showing an outline of the processing content of the operation guidance processing.
First, in S151, an operation guidance process for setting an initial state that needs to be performed on the microscope when an image of the sample 10 is acquired is performed.

Next, in S152, an operation guidance process for moving the electric XY stage 9 is performed to roughly set the observation position in the sample 10.
Next, in S153, an operation guidance process is performed for observing the sample 10 at a low magnification, which is performed in order to determine the capturing position of the image of the sample 10 to be acquired. Note that when a plurality of images of the sample 10 acquired while changing the capturing position are to be combined, an operation guidance process for setting a combining range in the image combining is also performed here.

  Next, in S154, an operation guidance process for setting basic control condition information in capturing the image of the sample 10 is performed. In the present embodiment, here, it is assumed that processing related to capturing a two-dimensional image of the sample 10 is performed. Further, here, an operation guidance process for observing the sample 10 at an observation magnification actually used for capturing the image of the sample 10 and determining the image capturing position is also performed.

  Next, in S155, an operation guidance process for setting applied control condition information in capturing the image of the sample 10 is performed. In the present embodiment, it is assumed here that the processing related to capturing the three-dimensional image of the sample 10 is performed.

  Next, in S156, the capturing of the image of the sample 10 based on the condition setting instructed by the user is started in accordance with the operation guidance performed as described above, and thereafter the processing of FIG. 7 ends.

The operation guidance process shown in FIG. 7 is performed according to the procedure as described above, and an optimum image of the sample 10 can be captured.
Next, the operation guidance process outlined in FIG. 7 will be described in more detail with reference to FIG.

  FIG. 8 is a table showing specific setting items set in each process shown in FIG. 7 and an example of an initial state for each setting item. That is, this table corresponds to information (guidance information) indicating the contents of an operation for performing settings when acquiring an image of the sample 10 to the microscope and information on conditions (condition information) set for the microscope. It is attached.

  Information represented in this table is stored in advance in a storage device. However, among the setting items in each column of the table, there may be condition information that is stored in the storage device in association with the setting item by the process of S104 of the condition information acquisition process illustrated in FIG. In such a case, as a general rule, the condition information is used in preference to that shown in the table of FIG. 8 as an initial state for the associated setting item.

  In each process described below, when an operation on the “next” button in the next process instruction unit 305 is detected by the controller 11, the condition information whose setting content is instructed in the operation guide screen 301 at that time is It is acquired by the controller 11 as for the setting item that has been guided in the process. Then, the controller 11 stores the condition information again in the storage device, updates the condition information, controls the microscope, performs various settings according to the acquired condition information, and then performs the following processing. Migrate to

  First, with respect to the operation guidance processing for setting the initial state in S151 in FIG. 7, the “guidance” in the table of FIG. It explains along.

  In FIG. 8, first, in a column 701, a screen for guiding the switching of the objective lens 5 (revolver 15) to the initial state is displayed on the operation guiding unit 304, and the objective lens adjusting unit 302 is further displayed on the operation guiding screen 301. This indicates that the processing to be displayed on is performed. Note that the selection of the objective lens 5 by this guidance is not final in the acquisition of the image of the sample 10, and therefore the condition information stored in the storage device is not used as the initial setting value. On the contrary, this selection emphasizes that the depth of focus is deep and easy to adjust the focus, and that the observation field is wide and easy to adjust the position. Therefore, unless otherwise specified, the lowest of the ones attached to the revolver 15 is not specified. It is desirable that the magnification objective lens 5 is set on the optical axis. Therefore, in the example of the operation guiding unit 304 shown in FIG. 3, as the initial state, the objective lens 5 of 5 × (5 ×) is selected according to the information of the initial state shown in the table of FIG. A sentence that induces is displayed. Of course, a configuration in which an instruction to select another objective lens 5 can be received by an operation on the objective lens adjustment unit 302 at this time may be employed.

  Next, in a column 702, a screen for guiding the Z revolver 13 to be moved in the optical axis direction to set the position to the initial state is displayed on the operation guiding unit 304, and further used for adjusting the position of the Z revolver 13. This indicates that processing for displaying the screen on the operation guide screen 301 is performed. In the example of FIG. 8, it is instructed as an initial state that the position of the Z revolver 13 after the movement is the uppermost movable position. Here, when the condition information about this position is extracted from the image data file and stored in the storage device, the condition information is read from the storage device and a screen showing the position is displayed on the operation guide screen 301. At the same time, a process of storing the position indicated by the condition information in the storage device as an initial state is performed.

  Next, in a column 703, a screen for guiding the enlargement magnification in the optical zoom function described above by the two-dimensional scanning unit 3 to the initial state is displayed on the operation guiding unit 304, and the optical zoom control unit 303 is further guided by the operation guidance. This indicates that processing to be displayed on the screen 301 is performed. Note that the optical zoom magnification setting by this guidance is not final in the acquisition of the image of the sample 10, and therefore the condition information stored in the storage device is not used as the initial setting value. On the contrary, in this selection, since the observation visual field is wide and position adjustment is easy, it is desirable to set the minimum magnification unless otherwise specified. Of course, a configuration may be adopted in which an instruction to set another magnification can be received by an operation on the optical zoom control unit 303 at this time.

  Next, in the column 704, acquisition of a microscope observation image is started and displayed on the observation image display unit 306 under the setting corresponding to the guidance by the processing represented in each column 701 to 703, and image observation is performed. This indicates that processing for causing the operation guiding unit 304 to display a screen for instructing to start the operation is performed.

Next, a column 705 indicates that processing for displaying a screen for guiding the sample 10 to be placed on the electric XY stage 9 on the operation guiding unit 304 is performed.
Next, a column 706 represents that processing for displaying a screen for guiding the rough adjustment of the focus on the operation guiding unit 304 while performing observation of the observation image displayed on the observation image display unit 306 is performed. Yes. In this screen, as a focus adjustment method, a method for adjusting the position of the Z revolver 13 and a focusing handle (not shown) mounted on the microscope main body 14 for moving the objective lens 5 up and down in the optical axis direction are provided. A message indicating that there is a method to be performed by operating may be displayed on the operation guiding unit 304.

The process so far is the operation guidance process for setting the initial state.
Next, with respect to the operation guidance process for the movement operation of the electric XY stage 9 in S152 in FIG. 7, the “guidance” in the table of FIG. A description will be given in the order of general processing.

  First, a column 711 indicates that processing for reading XY coordinates indicating the observation position of the sample 10 indicated in the coordinate file is performed with reference to a coordinate file prepared in advance. Here, when the condition information about the XY coordinates is extracted from the image data file and stored in the storage device, the condition information is read and a screen showing the position indicated by the condition information is displayed. A process of displaying the operation guidance screen 301 and storing this position in the storage device as an acquisition result of the XY coordinate setting instruction is performed.

  Next, in a column 712, an operation guidance display is displayed for guiding the alignment of the XY stage coordinate system to make the relationship between the installation state of the sample 10 and the current state of the system of FIG. 1 appropriate. This indicates that processing to be displayed on the unit 304 is performed. By this process, the alignment work procedure is also displayed on the display screen of the operation guidance display unit 304.

  Next, the column 713 displays a screen for guiding the selection of one of the XY coordinates read by the processing shown in the column 711 on the operation guiding unit 304, and for this selection A process for displaying a position selection GUI control screen (not shown) to be used on the operation guide screen 301 is performed. At this time, when a position is selected using the position selection GUI control screen, control is performed to move the electric XY stage 9 so that an observation image at that position is displayed on the observation image display unit 306. .

  Next, the column 714 guides the user to adjust the XY position of the electric XY stage 9 so that the portion desired to be observed in the sample 10 is the center of the observation image displayed on the observation image display unit 306. The process of displaying the screen to be displayed on the operation guiding unit 304 is performed. This adjustment instruction is performed using the position selection GUI control screen. When the instruction is given, control to move the electric XY stage 9 is performed in accordance with the instruction.

The process so far is the operation guidance process for moving the electric XY stage 9.
Next, regarding the operation guidance processing for observation at low magnification, which is S153 in FIG. 7, “guidance” in the table of FIG. 8 refers to each column in the column of “low magnification observation” in order from the top, A description will be given in the specific processing order.

  First, in the column 721, a screen for guiding the objective lens 5 (revolver 15) to be switched to a magnification higher than the initial state is displayed on the operation guiding unit 304, and the objective lens adjusting unit 302 is further operated. This shows that the processing to be displayed on the guidance screen 301 is performed. Note that the selection of the objective lens 5 by this guidance is not final in the acquisition of the image of the sample 10, and therefore the condition information stored in the storage device is not used as the initial setting value. That is, even in this selection, it is important that the depth of focus is deep and easy to adjust the focus and that the observation field is wide and easy to adjust the position, so unless otherwise specified, the conditions stored in the storage device It is desirable to select a magnification lower than the magnification indicated in the information. Of course, a configuration in which an instruction to select another objective lens 5 can be received by an operation on the objective lens adjustment unit 302 at this time may be employed.

  Next, a column 722 indicates that a process for adjusting the brightness of the observation image being displayed on the observation image display unit 306 to be appropriate is performed. As shown in the column 722, this adjustment is “automatic adjustment” in the initial state. In this case, the controller 11 is based on the amount of light detected by the photodetector 8. The detection sensitivity of the photodetector 8 is adjusted. Here, if the brightness of the observation image is set to be “manually adjusted”, as illustrated in FIG. 9, first, the brightness adjustment of the observation image and the instruction of “automatic adjustment” are performed. Processing for causing the operation guiding unit 304 to display a screen for guiding one of the above is performed. Further, a process of displaying the brightness adjustment GUI control screen 307 used for the adjustment or instruction on the operation guide screen 301 is performed. At this time, when the brightness after adjustment is instructed using the brightness adjustment GUI control screen 307, the photodetector 8 is displayed so that the observation image is displayed on the observation image display unit 306 at that brightness. The controller 11 performs processing for adjusting the detection sensitivity.

  Next, a column 723 indicates that processing for causing the operation guiding unit 304 to display a screen for guiding the focus adjustment while viewing the observation image displayed on the observation image display unit 306 is performed. As shown in the column 723, this adjustment is “AF” (autofocus) in the initial state. In this case, the controller 11 determines, for example, the contrast based on the contrast of the observation image. A control process for adjusting the position of the Z revolver 13 is performed so that is maximized. Here, when the user operates the input device and gives a predetermined instruction, it is possible to switch the setting so that the focus is adjusted by the “Z revolver”. In this case, the Z revolver moving GUI Processing for displaying a control screen (not shown) on the operation guide screen 301 is performed. At this time, when the Z revolver 13 is instructed to move using the Z revolver movement GUI control screen, the controller 11 performs a process for controlling the movement of the Z revolver 13 in accordance with the instruction.

The processing described so far is the operation guidance processing for observation at a low magnification.
Next, with respect to the operation guidance processing for basic control condition information setting, which is S154 in FIG. 7, “guidance” in the table of FIG. However, a description will be given along a specific processing order.

  First, in the column 731, when the image of the sample 10 is captured, a screen for instructing whether to acquire an image by the capturing method is displayed on the operation guiding unit 304, and the screen used for this selection is operated. Processing to be displayed on the guidance screen 301 is performed. In the example of FIG. 8, as this selection instruction, “snap” (snapshot shooting) for recording image data of the observation image displayed on the observation image display unit 306, and image data of the omnifocal image of the sample 10 are recorded “ Either “extend” (extended image capturing) or “film thickness” (film thickness image capturing) for recording a three-dimensional image suitable for measurement of the film thickness of the sample 10 that is a thin film is possible. Here, the example of FIG. 8 shows that “snap” is selected as the initial state of this selection. In addition, other types of images, for example, an image for combining a plurality of images of the sample 10 described above can be selected.

  Next, in the column 732, a screen for guiding the objective lens 5 (revolver 15) to switch to the magnification for image capture is displayed on the operation guiding unit 304, and the objective lens adjusting unit 302 is further displayed on the operation guiding screen. It represents that the processing to be displayed on 301 is performed. Here, when the condition information about the magnification of the objective lens 5 is extracted from the image data file and stored in the storage device, the condition information is first read from the storage device and indicated by the condition information. The objective lens 5 is switched to the magnification with the selected magnification, and a process for displaying a screen showing the switching result on the operation guide screen 301 is performed. Further, a process for newly storing the result of switching the objective lens 5 for capturing an image in the storage device is performed.

  Next, a column 733 represents that a process for adjusting the brightness of the observation image being displayed on the observation image display unit 306 to be appropriate is performed. As shown in the column 733, this adjustment is “automatic adjustment” in the initial state. In this case, the controller 11 is based on the amount of light detected by the photodetector 8. The detection sensitivity of the photodetector 8 is adjusted. Here, if the brightness of the observation image is set to be “manually adjusted”, as illustrated in FIG. 9, first, the brightness adjustment of the observation image and the instruction of “automatic adjustment” are performed. Processing for causing the operation guiding unit 304 to display a screen for guiding one of the above is performed. Further, a process of displaying the brightness adjustment GUI control screen 307 used for the adjustment or instruction on the operation guide screen 301 is performed. At this time, when the brightness after adjustment is instructed using the brightness adjustment GUI control screen 307, the photodetector 8 is displayed so that the observation image is displayed on the observation image display unit 306 at that brightness. The controller 11 performs processing for adjusting the detection sensitivity.

  Next, in the column 734, a process for causing the operation guiding unit 304 to display a screen for guiding the adjustment of the focus for actual image capturing while viewing the observation image displayed on the observation image display unit 306 is performed. Represents. As shown in the column 734, this adjustment is “AF” (autofocus) in the initial state. In this case, the controller 11 determines the contrast based on the contrast of the observation image, for example. A control process for adjusting the position of the Z revolver 13 is performed so that is maximized. Here, when the user operates the input device and gives a predetermined instruction, it is possible to switch the setting so that the focus is adjusted by the “Z revolver”. In this case, the Z revolver moving GUI Processing for displaying a control screen (not shown) on the operation guide screen 301 is performed. At this time, when the Z revolver 13 is instructed to move using the Z revolver movement GUI control screen, the controller 11 performs a process for controlling the movement of the Z revolver 13 in accordance with the instruction.

  Next, the column 735 causes the operation guide display unit 304 to display a screen that guides the adjustment of the enlargement magnification in the optical zoom function described above by the two-dimensional scanning unit 3 to that of actual image capture. It represents that the process which displays the control part 303 on the operation guidance screen 301 is performed. Here, when the condition information about the magnification of the optical zoom is extracted from the image data file and stored in the storage device, the condition information is first read from the storage device, and the magnification of the optical zoom is The process is set to that indicated by the condition information, and a process for displaying a screen showing the magnification on the operation guide screen 301 is performed. Further, a process for storing the magnification in the storage device as an optical zoom adjustment result is performed.

  It should be noted that the processing shown in each of the above columns 732 to 735 is repeatedly performed when the controller 11 detects an operation on the “return” button in the next process instruction unit 305 performed by the user as necessary. Sometimes it is.

  Next, in a column 736, the position where the observation image is captured in the sample 10 by adjusting the XY position of the electric XY stage 9 is the center of the observation image displayed on the observation image display unit 306. This shows that processing for causing the operation guiding unit 304 to display a screen for instructing to do so is performed. This adjustment instruction is performed using the position selection GUI control screen. When the instruction is given, control to move the electric XY stage 9 is performed in accordance with the instruction.

  Next, the column 737 displays a screen for guiding an instruction to set an image acquisition condition on the operation guide unit 304 when the image of the sample 10 is captured, and the screen used for this instruction is displayed on the operation guide screen 301. It represents that the process to display is performed. As an instruction for the acquisition condition, for example, “LSM” that is an instruction to acquire a laser scanning confocal image, “CCD” that is an instruction to capture a visible light observation image with a CCD imaging device, or “DIC” which is an instruction to acquire a differential interference observation image.

The above processing is the operation guidance processing for basic control condition information setting.
Next, with respect to the operation guidance process for setting the applied control condition information, which is the process of S155 in FIG. 7, “guidance” in the table of FIG. A description will be given along a specific processing order with reference to the drawings.

  First, the column 741 is a screen for guiding the setting of the accuracy in the height direction in capturing the three-dimensional image of the sample 10, that is, the acquisition interval of the confocal images of the plurality of samples 10 to be captured for generating the three-dimensional image. Is displayed on the operation guide unit 304, and the screen used for this instruction is displayed on the operation guide screen 301. Here, as an instruction for setting the height accuracy, a selection is made from “high accuracy” in which the confocal image acquisition interval is narrow, “medium accuracy” in which the acquisition interval is medium, and “low accuracy” in which the acquisition interval is wide. Shall. In the example of FIG. 8, “high accuracy” is selected as the initial state. Here, the condition information regarding the height accuracy may be extracted from the image data file and stored in the storage device. In this case, the condition information is read from the storage device, the confocal image acquisition interval is set to the one indicated by the condition information, and a screen indicating the acquisition interval is displayed on the operation guide screen 301. At the same time, the storage interval is newly stored in the storage device as the acquisition result of the accuracy setting in the height direction.

  Next, a column 742 shows a screen for guiding setting of a Z-direction (height direction) capture range in capturing a three-dimensional image of the sample 10, that is, a plurality of samples 10 captured for generating a three-dimensional image. The screen for guiding the setting of the Z-direction capture range of the confocal image is displayed on the operation guiding unit 304, and the screen used for this instruction is displayed on the operation guiding screen 301. Here, as the setting instruction for the capturing range, “automatic setting” for automatically determining the range when the image capturing operation is executed is set as an initial state. Here, when the user operates the input device and gives a predetermined instruction, it is possible to switch to “manual setting” in which the user specifies the upper and lower positions in the Z direction indicating the range. When the switching to “manual setting” is detected, the controller 11 causes the operation guiding unit 304 to display a screen for guiding the indication of the upper and lower limit positions in the Z direction indicating the range, and this guidance. The instruction of the position input in response to is acquired as the setting of the capturing range.

  Next, in the column 742, whether or not to automatically adjust the detection sensitivity of the photodetector 8 (adjustment by the controller 11) to make the brightness of the image appropriate for capturing the three-dimensional image of the sample 10 is performed. A screen for guiding the setting instruction is displayed on the operation guidance display unit 304, and processing for displaying a screen used for this instruction on the operation guidance screen 301 is performed. Here, as an initial state, the automatic adjustment of sensitivity is set to “not”. Here, when the user operates the input device and gives a predetermined instruction, the setting can be switched to “perform” automatic sensitivity adjustment. Here, when the condition information for the automatic adjustment of sensitivity is extracted from the image data file and stored in the storage device, the condition information is read from the storage device and the setting for the automatic adjustment is performed. Then, the process is performed as shown in the condition information, and a screen showing the setting is displayed on the operation guide screen 301.

The above processing is operation guidance processing for setting applied control condition information.
After the above processes are performed, as shown in the column 751 in the table of FIG. 8, the image of the sample 10 is started to be captured as the process of S156 of FIG. The guidance process ends.

  In the operation guidance process described above, the corresponding guidance process is performed for all items in the column shown in the table of FIG. Instead, for each item, it can be configured to select whether or not to execute the corresponding operation guidance process. It is also possible to configure such that the set conditions can be edited, such as addition or deletion of these items, change of the contents of the items, change of the order between the items. For this purpose, for example, the controller 11 reads out the table data from the storage device and performs a corresponding guidance process when executing the process of FIG. Here, the contents of this table data are edited using general-purpose spreadsheet software or the like. Further, with such a configuration, it is possible to perform different operation guidance for each user by creating unique table data for each user.

  Further, in the condition information acquisition process of FIG. 4, the condition information at the time of acquiring a microscope image acquired in the past is acquired from the image data file. Here, with respect to the operation condition information, the operation condition information may be further extracted from the image processing history information recorded in the image data file and stored in the storage device.

  Further, the image data file selection instruction screen illustrated in FIG. 5 may perform so-called thumbnail display as in the second example illustrated in FIG. When such thumbnail display is performed, the user can select an image suitable for acquisition of condition information while confirming a microscope image acquired in the past. Here, the condition information included in the image data file corresponding to the thumbnail display may be extracted, and the extracted condition information may be displayed as character information together with the thumbnail display. In this way, the user can check the condition information also with the character information, which is useful for preventing erroneous setting of the condition information.

Next, FIG. 11 will be described. FIG. 11 is a flowchart showing the processing contents of the second example of the condition information acquisition processing.
In the first example of the condition information acquisition process shown in FIG. 4, the condition information at the time of acquiring the microscope image acquired in the past is acquired from the image data file of the microscope image as a model in operation guidance, and the storage device Is stored. On the other hand, in this second example, when acquiring a microscope image from a report data file representing an observation report consisting of a microscope image acquired in the past and various image measurement processing results for the microscope image. The condition information (control condition information and operation condition information) is acquired as a model for operation guidance and stored in the storage device.

  An example of an observation report display screen that the controller 11 displays on the display unit 12 based on the report data file is shown in FIG. This display screen example includes an image display unit 901, an image information display unit 902, a profile line display unit 903, and a measurement result information display unit 904.

The image display unit 901 is a display unit for an image represented by an image data file included in (or associated with) a report data file.
The image information display unit 902 includes control condition information at the time of acquisition of the image displayed on the image display unit 901, the type of image measurement performed on the image (distance measurement between two points, volume measurement, etc.) and measurement conditions ( This is a display unit for operation condition information about the image, such as coordinate information and threshold information necessary for measurement. In addition, the operation condition information may include, for example, the type of image processing (for example, median filter or contrast enhancement processing) performed on the image.

  The profile line display unit 903 is a display unit for measurement information (for example, height information) on the profile line set for the image displayed on the image display unit 901.

The measurement result information display unit 904 is a display unit for displaying a result (measurement value or the like) of an image measurement process performed on the image displayed on the image display unit 901.
As described above, the report data file includes the image data file, the control condition information, the operation condition information, and the processing result information. Here, it is assumed that the control condition information is included in the header portion of the image data file as shown in FIG.

The process of FIG. 11 will be specifically described. This process is immediately started by the controller 11 by starting the above-described microscope system control program.
First, in S201, processing for displaying the user interface screen shown in FIG. 3 on the display unit 12 is performed.

  In S202, processing for acquiring an instruction of a report data file in which an observation report suitable for acquisition of condition information is represented is performed. In this process, first, detection processing of a predetermined operation guidance information creation instruction performed by the user is performed on the input device. When the instruction is detected, the image data file selection instruction illustrated in FIG. 5 or FIG. Display processing on the display unit 12 of the report data file selection instruction screen, which is the same mode as the screen, is performed. Then, detection processing of a predetermined report data file selection operation performed by the user on the input device is performed, and processing for deleting the display of the image data file selection instruction screen on the display unit 12 when the instruction is detected And a process of referring to the report data file according to the instruction.

  In S203, processing for extracting one piece of control condition information stored therein from the header portion of the image data file included (or associated) in the report data file being referenced by the processing in S202 Is done.

  In S204, the control condition information acquired by the process in S203 is stored in the storage device in association with the setting item in the operation guidance process (FIGS. 7 and 8) described above.

  In S205, a process of determining whether or not all the control condition information stored in the header portion of the image data file included in (or associated with) the report data file being referred to has been extracted is performed. Is called. If it is determined that all the control condition information has been extracted (when the determination result is Yes), the process proceeds to S206. On the other hand, when it is determined that unextracted control condition information remains (when the determination result is No), the process returns to S203, and the processes after S203 are performed on the unextracted control condition information.

In S206, a process of extracting one piece of operation condition information included and stored in the report data file being referred to is performed.
In S207, the operation condition information acquired by the process of S203 is stored in the storage device in association with the setting item in the operation guidance process (FIGS. 7 and 8) described above.

  In S208, processing is performed to determine whether or not all the operation condition information included and stored in the report data file being referred to has been extracted. If it is determined that all the operation condition information has been extracted (when the determination result is Yes), the process proceeds to S206. On the other hand, when it is determined that unextracted operation condition information remains (when the determination result is No), the process returns to S206, and the processes after S206 are performed on the unextracted operation condition information.

In S209, a process of shifting the operation state of the controller 11 to the operation guidance start state is performed, and thereafter the process of FIG. 11 is terminated.
The above processing is the second example of the condition information acquisition processing. When this process ends, the controller 11 that is executing the above-described microscope system control program subsequently starts the above-described operation guidance process. Then, the controller first performs the processing shown in FIGS. 7 and 8 to obtain guidance regarding setting of control condition information and an instruction regarding the setting. Then, following this processing, guidance relating to the setting of operation condition information and instructions relating to the setting are obtained by the manipulation guidance processing using the report data file shown in FIG. 13 in the same table as that of FIG.

  FIG. 13 will be described. FIG. 13 shows specific setting items added and set in the operation guidance process for the report data file, and an example of an initial state for each setting item.

  First, the operation guidance processing for image processing will be described in a specific processing order with reference to each column in the column “Image Processing” of “Guidance” in the table of FIG. Note that the example of FIG. 13 shows that image processing is executed twice and then image measurement is executed twice.

  In FIG. 13, first, as illustrated in FIG. 14, the column 801 displays a screen for guiding the setting of the first image processing type and the processing parameters on the operation guiding unit 304, and this setting. This shows that the processing for displaying the image processing setting screen 308 used for the operation on the operation guide screen 301 is performed. In this example, execution of “3 × 3” median filter processing is extracted from the report data file as operation condition information and stored in the storage device as the first image processing. That is, in this case, the operation condition information is read from the storage device, the image processing type is set in the “median filter” process indicated by the operation condition information, and the operation condition information is indicated by the operation condition information. “3 × 3” is selected as the processing parameter in this image processing. Of course, it is also possible to accept instructions for other types of image processing and processing parameters by operating the image processing setting screen 308 at this time. Here, when an operation on the “next” button in the next process instruction unit 305 is detected, the controller 11 performs the instructed image processing on the image of the sample 10 according to the instructed parameters.

  The column 802 displays a screen for guiding the setting of the second image processing type and the processing parameters on the operation guiding unit 304, and displays an image processing setting screen used for this setting. This indicates that processing to be displayed on the screen 301 is performed. In this example, as the second image processing, execution of “level 3” contrast enhancement processing is extracted from the report data file as operation condition information and stored in the storage device. That is, in this case, the operation condition information is read from the storage device, and the type of image processing is set in the “contrast enhancement” process indicated by the operation condition information, and is indicated by the operation condition information. “Level 3” (a value indicating the degree of contrast enhancement) is selected as a processing parameter in this image processing. Of course, it is also possible to accept instructions for other types of image processing and processing parameters by operating the setting screen at this time. Here, when an operation on the “next” button in the next process instruction unit 305 is detected, the controller 11 performs the instructed image processing on the image of the sample 10 according to the instructed parameters.

  In the column 811, as illustrated in FIG. 15, a screen for guiding the setting of the first image measurement type and the measurement condition parameters is displayed on the operation guide unit 304, and an image used for this setting is displayed. This shows that processing for displaying the measurement setting screen 309 on the operation guidance screen 301 is performed. In this example, as the first image measurement, the distance measurement of “Y: 512, X: 256-768” (on the sample 10 corresponding to the coordinates of (256,512) and (768,512) in the image) The execution of the measurement of the distance between positions is extracted from the report data file as operation condition information and stored in the storage device. That is, in this case, the operation condition information is read from the storage device, and the type of image measurement is set in “distance measurement” indicated by the operation condition information, and is indicated by the operation condition information. “Y: 512, X: 256-768” is selected as the measurement parameter in this image measurement. Of course, by operating the image measurement setting screen 309 at this time, other types of image measurement and measurement parameter instructions can be received. Here, when an operation on the “next” button in the next process instruction unit 305 is detected, the controller 11 performs the instructed image measurement on the image of the sample 10 according to the instructed parameters.

  In a column 812, a screen for guiding the setting of the second image measurement type and measurement condition parameters is displayed on the operation guide unit 304, and a setting screen used for this setting is displayed on the operation guide screen 301. It represents that the process to display is performed. In this example, as the second image measurement, the distance measurement of “Y: 256, X: 256-768” (on the sample 10 corresponding to the coordinates of (256,256) and (768,256) in the image) The execution of the measurement of the distance between positions is extracted from the report data file as operation condition information and stored in the storage device. That is, in this case, the operation condition information is read from the storage device, and the type of image measurement is set in “distance measurement” indicated by the operation condition information, and is indicated by the operation condition information. “Y: 256, X: 256-768” is selected as the measurement parameter in this image measurement. Of course, by operating the image measurement setting screen 309 at this time, other types of image measurement and measurement parameter instructions can be received. Here, when an operation on the “next” button in the next process instruction unit 305 is detected, the controller 11 performs the instructed image measurement on the image of the sample 10 according to the instructed parameters.

The operation guidance process for the report data file is performed as described above.
In the process for the report data file described above, both the control condition information and the operation condition information are acquired from the report data file as the condition information at the time of acquiring the microscope image, and stored in the storage device. Operation guidance was performed for the operation for setting. Instead of this, only the operation condition information may be acquired from the report data file and stored in the storage device, and the operation guidance for the operation for this setting may be performed.

  In the example of FIG. 13, all the condition operation information extracted from the report file is used. Instead of this, it is possible to adopt a configuration in which whether or not to use the condition operation information extracted from the report file can be selected for each item. In addition, instead of performing the corresponding guidance process for all the items shown in the table of FIG. 13, it is possible to select whether or not to execute the corresponding operation guidance process for each item. You can also. Furthermore, it is also possible to configure such that the set conditions can be edited, such as addition or deletion of these items, change of the contents of the items, change of the order between the items. For this purpose, for example, table data representing the table of FIG. 13 is stored in the storage device, and the controller 11 reads out this table data and executes the corresponding guidance when the operation guidance processing is executed. Do processing. Here, the contents of this table data are edited using general-purpose spreadsheet software or the like. Further, with such a configuration, it is possible to perform different operation guidance for each user by creating unique table data for each user.

  As described above, according to the microscope system of FIG. 1, even a user unfamiliar with the microscope can easily adjust (condition setting) the optimum microscope control unit according to the characteristics of the sample 10. In addition, since a screen showing the procedure of the operation performed on the microscope in order to acquire the image of the sample 10 is displayed according to the order of the operation and the operation is guided, the microscope is operated according to the guidance. The user can learn how to operate the microscope by repeatedly performing the operation of acquiring the image of the sample 10.

  In addition, from the image data file about the image acquired in the past by the microscope, or from the report data file containing the observation report data about the image, to acquire the condition information at the time of acquisition of the image, Since the setting according to the condition information is performed on the microscope, the image of the sample 10 can be acquired under the same conditions as when acquiring an image acquired in the past.

  As mentioned above, although embodiment of this invention was described, this invention is not limited to each embodiment mentioned above, A various improvement and change are possible within the range which does not deviate from the summary of this invention.

It is a figure which shows the structure of the microscope system which implements this invention. It is a figure which shows an example of the cross-sectional shape of a sample. It is a figure which shows the example of a screen of a user interface screen (the 1). It is the figure which showed the processing content of the condition information acquisition process with the flowchart. It is a figure which shows the 1st example of a screen of an image data file selection instruction | indication screen. It is a figure which shows the example of condition information. It is the figure which showed the outline | summary of the processing content of the operation guidance process with the flowchart. It is the figure which displayed the example of the initial state with respect to the specific setting item set in each process shown in FIG. 7, and each setting item. It is a figure which shows the example (2) of a user interface screen. It is a figure which shows the 2nd example of an image data file selection instruction | indication screen. It is the figure which showed the processing content of the 2nd example of condition information acquisition processing with the flowchart. It is a figure which shows the example of a display screen of an observation report. It is the figure which displayed the example of the initial state with respect to the specific setting item added and set in the operation guidance process for a report data file, and each setting item. It is a figure which shows the example of a screen of a user interface screen (the 3). It is a figure which shows the example (4) of a user interface screen.

Explanation of symbols

1 Laser light source 2 PBS
3 Two-dimensional scanning unit 4 1 / 4λ plate 5 Objective lens 6 Imaging lens 7 Pinhole 8 Photo detector 9 Electric XY stage 10 Sample 11 Controller 12 Display unit 13 Z revolver 14 Microscope main body 15 Revolver 301 Operation guide screen 302 Objective lens Adjustment unit 303 Optical zoom control unit 304 Operation guidance unit 305 Next process instruction unit 306 Observation image display unit 307 Brightness adjustment GUI control screen 308 Image processing setting screen 309 Image measurement setting screen 901 Image display unit 902 Image information display unit 903 Profile Line display section 904 Measurement result information display section

Claims (5)

Guidance information, which is information indicating the contents of an operation for performing settings when acquiring an image of a target sample to the microscope, and condition information, which is information on conditions set for the microscope, are stored in association with each other. A storage unit;
An operation guide unit that guides the operation by displaying an operation guide display screen representing the content of the guide information stored in the storage unit according to the order of the operation;
A condition information display unit that displays a screen representing the content of the condition information stored in the storage unit in association with the guidance information related to the operation guidance display screen displayed by the operation guidance unit;
A condition information instruction acquisition unit that acquires an instruction of the condition information and updates the condition information stored in the storage unit with the condition information related to the instruction;
A control unit for controlling the microscope and performing setting for the microscope according to the condition information stored in the storage unit;
A microscope system comprising:   The microscope system according to claim 1, wherein the storage unit stores the condition information when an image acquired in the past by the microscope is acquired. A method of acquiring the condition information at the time of acquisition of the image from an image data file of an image acquired in the past by the microscope or from a report data file including observation report data of the image It further has this condition information acquisition unit,
The storage unit stores the condition information acquired by the example condition information acquisition unit.
The microscope system according to claim 2.   The condition information instruction acquisition unit is associated with the guide information related to the operation guide display screen based on the operation guide display screen displayed by the operation guide unit when the instruction of the condition information is acquired and stored in the memory. The microscope system according to claim 1, wherein the condition information stored in the unit is updated to condition information related to the acquired instruction. A program for causing a computer to control a microscope,
In the storage unit, guidance information, which is information indicating the content of an operation for performing settings when acquiring an image of a target sample to the microscope, and condition information, which is information on conditions set for the microscope, are associated with each other. A storage process to be stored;
An operation guidance process for guiding the operation by displaying an operation guidance display screen representing the content of the guidance information stored in the storage unit on the display unit according to the order of the operation;
A condition information display process for causing the display unit to display a screen representing the content of the condition information stored in the storage unit in association with the guidance information related to the operation guidance display screen displayed by the operation guidance process;
A condition information instruction acquisition process for acquiring an instruction of the condition information and updating the condition information stored in the storage unit with the condition information related to the instruction;
A control process for controlling the microscope and performing setting for the microscope according to the condition information stored in the storage unit;
A microscope control program for causing the computer to perform the operation.