JP2009504190A5 - - Google Patents

Download PDF

Info

Publication number
JP2009504190A5
JP2009504190A5 JP2008527139A JP2008527139A JP2009504190A5 JP 2009504190 A5 JP2009504190 A5 JP 2009504190A5 JP 2008527139 A JP2008527139 A JP 2008527139A JP 2008527139 A JP2008527139 A JP 2008527139A JP 2009504190 A5 JP2009504190 A5 JP 2009504190A5
Authority
JP
Japan
Prior art keywords
strand
nucleotide
nucleic acid
double
acid molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2008527139A
Other languages
Japanese (ja)
Other versions
JP2009504190A (en
Filing date
Publication date
Priority claimed from US11/205,646 external-priority patent/US20080161256A1/en
Priority claimed from US11/234,730 external-priority patent/US20070270579A1/en
Priority claimed from US11/299,254 external-priority patent/US20060217331A1/en
Application filed filed Critical
Priority claimed from PCT/US2006/032168 external-priority patent/WO2007022369A2/en
Publication of JP2009504190A publication Critical patent/JP2009504190A/en
Publication of JP2009504190A5 publication Critical patent/JP2009504190A5/ja
Pending legal-status Critical Current

Links

Claims (5)

センス鎖とアンチセンス鎖を含む構造ＳＸＩＩを有する二本鎖核酸分子：
Figure 2009504190
 ［ここで、二本鎖核酸分子の上側の鎖はセンス鎖であり、下側の鎖はアンチセンス鎖であり、前記アンチセンス鎖は、標的ＲＮＡに相補的である配列を含み、各Ｎは独立にヌクレオチドであり、各Ｂは存在しても、しなくても良い末端キャップ部分であり、（Ｎ）は、無修飾でも、化学修飾されていても良い、非塩基対形成ヌクレオチド又はオーバーハングヌクレオチドであり、［Ｎ］はリボヌクレオチドであるヌクレオチド位置であり、Ｘ１及びＸ２は独立に約０〜約４の整数であり、Ｘ３は約９〜約３０の整数であり、Ｘ４は約１１〜約３０の整数であって、但しＸ４とＸ５の合計は約１７〜３６であり、Ｘ５は約１〜約６の整数であり、ならびに
（ａ）アンチセンス鎖中に存在する任意のピリミジンＮヌクレオチドは２’−デオキシ−２’−フルオロヌクレオチドであり、アンチセンス鎖中に存在する任意のプリンＮヌクレオチドは２’−Ｏ−メチルヌクレオチドであり、
（ｂ）センス鎖中に存在する任意のピリミジンＮヌクレオチドは２’−デオキシ−２’−フルオロヌクレオチドであり、センス鎖中に存在する任意のプリンＮヌクレオチドはデオキシリボヌクレオチドであり、および
（ｃ）任意の（Ｎ）ヌクレオチドは、２’−Ｏ−メチル、２’−デオキシ−２’−フルオロ又はデオキシリボヌクレオチドであっても良い］。 Double-stranded nucleic acid molecule having structure SXII comprising a sense strand and an antisense strand:
Figure 2009504190
 [Wherein the upper strand of the double-stranded nucleic acid molecule is the sense strand, the lower strand is the antisense strand, and the antisense strand comprises a sequence that is complementary to the target RNA, each N being Independently nucleotides, each B is an end cap moiety that may or may not be present, (N) is a non-base paired nucleotide or overhang, which may be unmodified or chemically modified [N] is a nucleotide position that is a ribonucleotide, X1 and X2 are independently an integer from about 0 to about 4, X3 is an integer from about 9 to about 30, and X4 is from about 11 An integer of about 30, wherein the sum of X4 and X5 is about 17-36, X5 is an integer of about 1 to about 6, and (a) any pyrimidine N nucleotide present in the antisense strand Is 2'-deoxy- '- fluoro nucleotides, any purine N nucleotides present in the antisense strand are 2'-O- methyl nucleotides,
(B) any pyrimidine N nucleotide present in the sense strand is a 2′-deoxy-2′-fluoro nucleotide, any purine N nucleotide present in the sense strand is a deoxyribonucleotide, and (c) any The (N) nucleotide may be 2′-O-methyl, 2′-deoxy-2′-fluoro or deoxyribonucleotide]. Ｘ５＝１、２又は３；各Ｘ１及びＸ２＝１又は２；Ｘ３＝１２、１３、１４、１５、１６、１７、１８、１９、２０、２１、２２、２３、２４、２５、２６、２７、２８、２９又は３０、並びにＸ４＝１５、１６、１７、１８、１９、２０、２１、２２、２３、２４、２５、２６、２７、２８、２９又は３０である、請求項１に記載の二本鎖核酸分子。   X5 = 1, 2 or 3; X1 and X2 = 1 or 2; X3 = 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27 28, 29 or 30 and X4 = 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30. Double-stranded nucleic acid molecule. Ｂが、センス鎖の３’及び５’末端に存在する、請求項１に記載の二本鎖核酸分子。   2. The double stranded nucleic acid molecule of claim 1, wherein B is present at the 3 'and 5' ends of the sense strand. ｓｉＮＡ分子のセンス鎖、アンチセンス鎖又はセンス鎖とアンチセンス鎖の両方の３’末端上の第１の末端（Ｎ）における１個以上のホスホロチオアートヌクレオチド間結合を含む、請求項１に記載の二本鎖核酸分子。   2. One or more phosphorothioate internucleotide linkages at the first end (N) on the 3 ′ end of the sense strand, antisense strand, or both sense and antisense strands of the siNA molecule. The double-stranded nucleic acid molecule as described. 薬剤として許容される担体又は希釈剤中に請求項に記載１の二本鎖核酸分子を含む、組成物。   A composition comprising the double-stranded nucleic acid molecule of claim 1 in a pharmaceutically acceptable carrier or diluent.
JP2008527139A 2005-08-17 2006-08-17 Chemically modified small interfering nucleic acid molecules that mediate RNA interference Pending JP2009504190A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11/205,646 US20080161256A1 (en) 2001-05-18 2005-08-17 RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US11/234,730 US20070270579A1 (en) 2001-05-18 2005-09-23 RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US73702405P 2005-11-15 2005-11-15
US11/299,254 US20060217331A1 (en) 2001-05-18 2005-12-08 Chemically modified double stranded nucleic acid molecules that mediate RNA interference
PCT/US2006/032168 WO2007022369A2 (en) 2005-08-17 2006-08-17 Chemically modified short interfering nucleic acid molecules that mediate rna interference

Publications (2)

Publication Number Publication Date
JP2009504190A JP2009504190A (en) 2009-02-05
JP2009504190A5 true JP2009504190A5 (en) 2011-02-24

Family

ID=40328218

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008527139A Pending JP2009504190A (en) 2005-08-17 2006-08-17 Chemically modified small interfering nucleic acid molecules that mediate RNA interference

Country Status (6)

Country Link
JP (1) JP2009504190A (en)
BR (1) BRPI0614407A2 (en)
IL (1) IL189540A0 (en)
MX (1) MX2008002369A (en)
NO (1) NO20081376L (en)
NZ (1) NZ565712A (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009086174A1 (en) 2007-12-21 2009-07-09 Srs Labs, Inc. System for adjusting perceived loudness of audio signals
JP5730508B2 (en) * 2009-07-10 2015-06-10 アンジェスＭｇ株式会社 Treatment or prevention agent for inflammatory bowel disease
US8538042B2 (en) 2009-08-11 2013-09-17 Dts Llc System for increasing perceived loudness of speakers
US9312829B2 (en) 2012-04-12 2016-04-12 Dts Llc System for adjusting loudness of audio signals in real time

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050233996A1 (en) * 2002-02-20 2005-10-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hairless (HR) gene expression using short interfering nucleic acid (siNA)
AU2003207708A1 (en) * 2002-02-20 2003-09-09 Sirna Therapeutics, Inc. Rna interference mediated inhibition of map kinase genes

Similar Documents

Publication Publication Date Title
JP2008283975A5 (en)
JP2005517432A5 (en)
JP2006502694A5 (en)
JP2005517427A5 (en)
JP2005517452A5 (en)
JP2005524393A5 (en)
JP6352269B2 (en) Improved oligomers with off-target profiles
JP2005517430A5 (en)
JP2005517438A5 (en)
JP2013535212A5 (en)
JP2005517433A5 (en)
JP2005518803A5 (en)
JP2010525813A5 (en)
JP2009060893A5 (en)
JP2005517436A5 (en)
JP2005500025A5 (en)
JP2009514877A5 (en)
JP2009524419A5 (en)
JP2017093448A5 (en)
JP2012502657A5 (en)
JP2019503394A5 (en)
JP2018512041A5 (en)
JP2014527401A5 (en)
JP2018512110A5 (en)
CA2457528A1 (en) Rna interference mediated inhibition of hepatitis c virus (hcv) gene expression using short interfering nucleic acid (sina)