JP2009256225A - Cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic without having the smell or coloration unique to a fish, further having an excellent skin-beautifying effect by preventing the damage of skin and maintaining a young and healthy state of the skin, and also provide an extract to be contained in such the cosmetic. <P>SOLUTION: This cosmetic is characterized by containing an extract from the bladder of a sturgeon, and further the extract from the bladder of the sturgeon is extracted after hydrolyzing collagen contained in the bladder of the sturgeon or while hydrolyzing the same. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cosmetic containing an extract from a sturgeon float bag, and more particularly to a cosmetic having a beautifying effect.

  Collagens, which are the main constituent proteins of animals, have been widely used as adhesives (glue) for a long time, and have also been used in a wide range of fields such as photographic emulsions, papermaking, dyeing, foods, cosmetics, and pharmaceuticals.

  The tissue mainly used for obtaining collagen is calf skin, and collagen extracted from pigmentless fish and fish skin is also disclosed (Patent Document 1 and Patent Document 2). However, obtaining collagen from animals such as calves and fish is disadvantageous for industrial production in that respect because the processing burden and the proportion of extract for removing skin and subcutaneous tissue are relatively small.

  Patent Document 3 describes marine collagen extracted from each tissue of fish (cod, flounder, salmon, sardine, tuna, etc.) that are marine organisms, and examples of the tissue include a float. . Further, Patent Documents 4 to 6 describe collagen extracted from skin such as cocoons, gold eyelids, larvae, flounder and the like or floating bags.

  However, in general, collagen extracted from fish has problems such as a taste and smell peculiar to fish and easy coloring. Therefore, in the use of cosmetic raw materials such as cosmetic compositions, it is not used because the odor causes unpleasant feeling at the time of use. There was a limit, and the effects of collagen (moisturizing properties, skin modifying properties, etc.) could not be fully exhibited.

  Therefore, various collagen extraction methods have been disclosed as means for solving the fishy smell peculiar to fish-derived collagen, but all of these methods have problems such as low yields, It was not suitable for production of the used product (for example, Patent Document 7 to Patent Document 9).

  In addition, there are few types and quality of collagen itself, and it has never been sufficient for the purpose of inclusion in cosmetics having excellent skin-beautifying effects. On the other hand, the demand for cosmetics capable of maintaining a youthful skin condition is increasing, and the above-mentioned known technology is insufficient to answer the demand, and further development has been desired.

  On the other hand, sturgeon floats are only used as wine and beer fermentation stoppers, art repairs, and adhesives for Western musical instruments such as violins, and have never been used in the cosmetics field.

JP 2002-226356 A JP 2003-238597 A JP 2003-089616 A JP 2003-192565 A JP 2003-192666 A JP 2003-192567 A JP-A-5-093000 Japanese Patent Laid-Open No. 5-125100 JP-A-5-155900

  This invention is made | formed in view of the said background art, The subject is preventing skin damage and providing the cosmetics which have the outstanding skin effect, such as maintaining a youthful and healthy skin state. Furthermore, it is to provide a cosmetic that does not have a fish-specific odor or coloring.

  As a result of intensive studies to solve the above-mentioned problems, the present inventor has found that the above-mentioned problems can be achieved by a cosmetic containing an extract from a sturgeon float bag, and has completed the present invention. It was.

  That is, the present invention provides a cosmetic comprising an extract from a sturgeon float bag.

  The present invention also provides an extract from sturgeon float bags contained in the cosmetic.

  According to the present invention, it is possible to provide a cosmetic that prevents skin damage, realizes a youthful and healthy skin condition, and has an excellent skin beautifying effect. Specifically, “beautifying skin effect” refers to an increase in skin moisture or prevention of lowering skin moisture, prevention of increase in skin elasticity or decrease in skin elasticity, lightening of skin color or dullness of skin color, spots and wrinkles. It means prevention.

  Further, according to the present invention, it is possible to provide a cosmetic containing “an extract containing an active ingredient such as collagen” from a floating bag, which has no fish-specific odor, is not colored, and does not give discomfort during use.

  Hereinafter, the present invention will be described. However, the present invention is not limited to the following embodiments, and can be arbitrarily modified and implemented.

  The present invention is a cosmetic comprising an extract from a sturgeon float bag. In the present invention, “sturgeon” refers to fish belonging to the order Sturgeon, Sturgeonidae. There are about 30 species of natural or farmed sturgeon, all of which are currently identified, any of which can be used. Specific examples thereof, Acipenser baeri, Acipenser brevirostrum, Acipenser dabryanus, Acipenser fulvescens, Acipenser gueldenstaedti, Acipenser mediradoi, Acipenser naccarii, Acipenser nudiventris, Acipenser oxyrinchus, Acipenser persicus, Acipenser ruthenus, Acipenser schrencki, Acipenser sinensis, Acipenser stellatus, Acipenser sturio, Acipenser transmontanus Huso dauricus, Huso huso, Pseudoscaphirhynchus fedtschenkoi, Pseudoscaphirhynchus hermannii, Pseudoscaphirhynchus kaufmanni, Scaphirhynchus albus, Scaphirhynchus platorynchus, Scaphirhynchus suttkusi and the like.

  The floating bag used in the present invention may be any of the sturgeon floating bags, but aquaculture technology has been established that it is a floating bag of Amur sturgeon, white sturgeon, siberian sturgeon, sturgeon or vestel sturgeon. It is preferable in terms of protection. Vestel sturgeon is a kind of sturgeon artificially created from a female Beluga sturgeon and a male sturgeon, and is made by crossing such an F1 Vaster sturgeon with an F1 Vaster sturgeon, a Beluga sturgeon or a sturgeon F2 Vaster Sturgeon is also called “Vaster Sturgeon”. In the present invention, any floating bag is preferable. The sturgeon is a Japanese name, also known as Aspensar South in scientific name and Surluyazi in dew.

  A “buoy bag” is a balloon-like organ in the body of a fish that is highly stretchable, and adjusts the buoyancy by storing or withdrawing air. In addition, the sturgeon float bag contains collagen substantially composed only of type I collagen, and its molecular weight is said to be about 300,000.

  The sturgeon float bag used in the present invention may be removed from the killed sturgeon for the purpose of the present invention, but it is used for applications other than the present invention, specifically for edible use including egg collection, glue use, etc. For this reason, the latter may be taken out from the sturgeon killed, and the latter is preferable in terms of protecting the natural environment.

  There is no particular limitation on the timing of obtaining the extract from the sturgeon float bag, but the sturgeon float bag to be used is fresh, so it is either immediately after being taken out from the sturgeon or taken out from the sturgeon. It is preferable that it is frozen immediately after that. When extracted from a floating bag that has not been immediately frozen, the extract has a raw odor, and the cosmetics containing it have a raw odor and may not be used as a cosmetic. Furthermore, the extract may change color over time or insoluble matter may precipitate over time. It is particularly preferable that the bag is frozen immediately after it is taken out, and further extracted from a bag that is always kept frozen until the extraction operation. “Immediately after freezing after removal from sturgeon” usually means within 2 hours, preferably within 1 hour, and more preferably within 30 minutes. The freezing temperature is not particularly limited, but is preferably -80 ° C to -20 ° C. Moreover, it is preferable to perform the following process immediately after thawing.

  The extract from the sturgeon buoy in the present invention is obtained by extracting the extract from the sturgeon buoy using a solvent as it is, or after hydrolyzing the collagen contained in the sturgeon buoy or after hydrolysis. An extract obtained by extraction using a solvent.

  Prior to extraction, it is preferable to remove extraneous matter, blood and other deposits from the sturgeon float. When removing by washing, the liquid used for washing preferably includes water, physiological saline, ethanol, or a mixture thereof. Further, the sturgeon float bag is preferably used after being cut, shredded, crushed or minced by an appropriate means in order to increase the extraction efficiency. At that time, a known crushing device, mincing device, and shredding device can be used. Either a dry method or a wet method may be used, but the wet method is preferable because it is more efficient and less deteriorated or damaged. It is also preferable to perform the above operation after drying. Preferable drying methods include freeze drying, natural drying, hot air drying and the like. Specifically, for example, a preferable drying temperature is −80 ° C. to 120 ° C., and particularly preferably −30 ° C. to 80 ° C.

  In addition, the extract from the sturgeon float bag according to the present invention is preferably an extract from ising glass from the viewpoints of ease of extraction, ease of storage in the previous stage of extraction, and the like. “Isinglass” is a fish bag that may be processed to reduce the molecular weight, and is mechanically processed into a solid state such as a translucent sheet. In the present invention, it is also preferable that sturgeon floats are once processed into Aisin glass and an extract therefrom is used.

  There is no particular limitation on the method for extracting the sturgeon bag with a solvent, and a generally known method may be used. For example, immersion extraction, Soxhlet extraction, etc. are mentioned. Among these, immersion extraction is preferable because of cost advantage. In immersion extraction, the presence or absence of stirring is not particularly limited, but it is also preferable not to perform steady stirring in consideration of productivity.

  There is no restriction | limiting in particular as a solvent used for the extraction from the sturgeon float bag, Water or an organic solvent can be used. Specifically, for example, water, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, hexanol, heptanol, 2-ethylhexanol, n-octanol, etc. Alcohols or water-containing lower alcohols; glycerin, propylene glycol, 1,3-butylene glycol, hexanediol, pentanediol, dipropylene brickol and other C2-C8 polyhydric alcohols or water-containing polyhydric alcohols; acetic acid C3-C8 lower esters such as methyl, ethyl acetate, propyl acetate, methyl propionate, ethyl propionate, methyl butyrate, and ethyl butyrate, carbon numbers such as benzene, toluene, n-pentane, n-hexane, and cyclohexane 5-10 hydrocarbons; aceto , Methyl ethyl ketone, methyl butyl ketone, 2 to 8 carbon atoms such as cyclohexanone; dimethyl ether, diethyl ether, ethers having 2 to 6 carbon atoms such as tetrahydroborate furan; acetonitrile; and the like as preferred.

  Among these, water and / or a hydrophilic organic solvent is preferable in that it is easy to operate, has little toxicity, and has no problem even if the solvent is distilled off and replaced with a cosmetic solvent. . Here, the hydrophilic organic solvent means a solvent compatible with water at a certain ratio at 30 ° C., but a solvent compatible with water at an arbitrary ratio is more preferable. Among these, ethanol, propylene glycol, 1,3-butylene glycol, 1,2-hexanediol, pentanediol, glycerin and the like are particularly preferable, and water, ethanol or 1,3-butylene glycol is more preferable from the above viewpoint.

  One or more selected from the above solvents can be mixed and used as a solvent. However, when it is not preferable to contain an organic solvent depending on the application, in the case of hydrolyzing with an enzyme as described below, only water is used, or a low-boiling solvent that is easily distilled off after extraction, or those An arbitrary mixed solvent of water and water can also be used. In the present invention, it is particularly preferable to perform extraction with water, a hydrophilic organic solvent, or “a mixed solvent of water and a hydrophilic organic solvent”.

  Extraction with a solvent and then solvent replacement by a known method is preferable because the solvent most suitable for extraction and the solvent suitable for cosmetics are often different. The solvent used for the extraction is not particularly limited as long as it can be efficiently extracted from the bag, and examples thereof include the same solvents as described above, but the boiling point at 1 atm is 50 ° C. to 80 ° C. A low-boiling point solvent is preferable from the viewpoint of easy distillation. Examples of such a low boiling point solvent include methanol, ethanol, hexane, acetone and the like.

  The solvent to be added after the solvent used for extraction is completely or almost distilled off is not particularly limited as long as it is known as a solvent contained in cosmetics as it is, but water, ethanol, propylene glycol 1,3-butylene glycol, 1,2-hexanediol, pentanediol, glycerin and the like are preferable.

  It is also preferable that the collagen contained in the sturgeon float bag is hydrolyzed or extracted using a solvent while hydrolyzing. Collagen contained in sturgeon floats has a high molecular weight, so it can be dissolved in a solvent by hydrolysis or extraction while hydrolyzing, and the stability of the cosmetics to be blended, to the skin It is preferable to add a hydrolysis step for the permeation property and the like. The smaller the molecular weight, the better the skin penetration, and the better the skin effect.

  The method for hydrolysis is not particularly limited, and a conventional method can be used. Specifically, for example, a method of heat treatment under acidic or alkaline conditions, a method of treatment with an enzyme, a method of heat extraction by adding water, a method of pressure heat extraction by adding water, a method of combining them, etc. Can be mentioned. Preferably, it is a method of heat treatment under acidity or alkalinity, a method of treatment using an enzyme, and more preferably a method of treatment using an enzyme from the viewpoint of uniformity of product quality and ease of process control. is there.

  After hydrolyzing, it may be extracted from there with another solvent, or the hydrolyzed solution may be subjected to treatments such as concentration, neutralization, enzyme deactivation and the like as it is as the “extract” of the present invention. In the “extract from sturgeon buoyancy bag” of the present invention, the extracts obtained by these methods can be used singly or in combination of two or more.

  A method for hydrolyzing collagen contained in the sturgeon float bag according to the present invention will be described below. In the method of heat treatment under acidity or alkalinity, the acid used is not particularly limited as long as the pH can be adjusted to 5 or less, preferably 4 or less. Specifically, hydrochloric acid, sulfuric acid, nitric acid can be used. Inorganic acids such as acetic acid, propionic acid, citric acid, lactic acid, ethylenediaminetetraacetic acid and the like.

  As pH of the liquid at the time of hydrolyzing acidic, 0.5-5.5 are preferable, 1.0-4.5 are more preferable, 1.0-3.0 are especially preferable.

  On the other hand, the alkali is not particularly limited as long as the pH can be adjusted to 9 to 13, and specifically, hydroxides such as sodium hydroxide, potassium hydroxide, barium hydroxide, ammonium hydroxide; Carbonic acid (hydrogen) salts such as sodium carbonate, potassium carbonate, ammonium carbonate, sodium hydrogen carbonate, potassium hydrogen carbonate; ethylenediamine tetraacetic acid disodium, ethylenediamine tetraacetic acid tetrasodium, etc. In view of this, alkali metal hydroxides such as sodium hydroxide and potassium hydroxide; carbonate (hydrogen) salts such as sodium carbonate and sodium hydrogen carbonate are preferred.

  The pH of the alkaline solution for hydrolysis is preferably 8.0 to 14.0, more preferably 8.5 to 13.5, and particularly preferably 9.0 to 13.0.

  Using the above acid or alkali, preferably at 0 ° C. to 120 ° C., more preferably 60 ° C. to 90 ° C., particularly preferably 70 ° C. to 80 ° C., preferably 0.5 Normal operations such as stirring are performed for a time to 24 hours, more preferably 1 to 6 hours.

  In the method for treating a sturgeon float in the present invention with an enzyme, the enzyme used is not particularly limited as long as it is a proteolytic enzyme. The “proteolytic enzyme” in the present invention is a general term for enzymes that catalyze the hydrolysis of peptide bonds, and is called peptidase or protease. Peptidases are classified into narrowly defined peptidases and proteinases. Peptidases (proteases) are classified into exopeptidases and endopeptidases according to the mode of action, and specifically include aminopeptidases, dipeptidases, dipeptidyl peptidases, tripeptidyl peptidases, carboxypeptidases. Such as exopeptidase, serine endopeptidase (serine protease, trypsin, chymotrypsin, etc.), cysteine endopeptidase (cysteine protease, thiol protease, papain, chymopapain, etc.), aspartate endopeptidase (acidic protease, pepsin, etc.), Examples include endopeptidases such as metalloendopeptidases (metal protease, carboxypeptidase, etc.). Among these, actinase, pepsin, trypsin, papain, neurase, collagenase, gelatinase, bromelain, and pancreatin are preferable, and trypsin is more preferable because an active ingredient can be satisfactorily extracted. These enzymes can be used alone or in combination of two or more.

  The method for treating the sturgeon float bag of the present invention with an enzyme may be a commonly used method and is not particularly limited. The conditions for reacting the enzyme with the sturgeon bag may be appropriately adjusted according to the enzyme used. For example, the sturgeon bag is immersed in water and usually 10 minutes to 24 hours, preferably 1 hour to 6 hours. What is necessary is just to make it react for time, More preferably 3 hours-5 hours. If the reaction time is too long, discoloration or a strange odor may occur, or the cosmetic containing the extract may lack stability. On the other hand, if it is too short, the enzyme may not work sufficiently.

  A preferred temperature is 20 ° C to 80 ° C, particularly preferably 25 ° C to 60 ° C. Moreover, the organic or inorganic acid or salt used normally can be used for pH adjustment. The amount of the enzyme used is usually 0.0001 parts by mass to 1 part by mass, preferably 0.001 parts by mass to 0.1 parts by mass with respect to 100 parts by mass of the sturgeon float bag. If the amount of the enzyme used is too small, the target extract may not be obtained efficiently, and if it is too much, discoloration or off-flavor may occur. The treatment using the enzyme is not limited to once, and may be performed a plurality of times.

  After the treatment with the enzyme, it is preferable to deactivate the enzyme by heat treatment or the like. The heating temperature is preferably 75 ° C to 100 ° C, particularly preferably 80 ° C to 90 ° C. The heating time is preferably 5 minutes to 2 hours, particularly preferably 10 minutes to 1 hour. If the deactivation conditions are too strong, discoloration and a strange odor may occur, and when used as a cosmetic, the color tone and scent may be abnormal. On the other hand, if the inactivation condition is too weak, the proteolytic ability may remain, and skin troubles such as fogging may occur on the skin when used as a cosmetic.

  Before the hydrolysis treatment, deashing treatment may be performed for the purpose of removing unnecessary components such as inorganic substances in advance. The decalcification treatment may be performed by using a usual method such as stirring for 24 to 48 hours using the above-described acid, and the amount of these used may be appropriately set and is not particularly limited. Moreover, you may perform several times as needed, and also may wash with water after demineralization. Moreover, you may perform a degreasing process before processing using an enzyme. The degreasing treatment can be performed using, for example, an organic solvent treatment solution such as ethanol or acetone, an alkali treatment solution such as sodium hydroxide or potassium hydroxide, or a surfactant treatment solution such as a nonionic surfactant. These usage amounts may be appropriately set and are not particularly limited. The method of degreasing treatment is not particularly limited, and for example, it may be performed by a usual method such as stirring sturgeon floats in the treatment solution for 3 to 48 hours. Moreover, you may perform several times as needed, and also may wash with water after a deashing process.

  What is necessary is just to collect | recover the extract from the sturgeon float bag by the collection technique after a general hydrolysis after said hydrolysis process. For example, separation and recovery may be performed by filter filtration, centrifugation, or the like. Thereafter, if necessary, purification may be performed using, for example, ion exchange resin, activated carbon, dialysis, ultrafiltration, etc., which is dissolved and recovered again in purified water, or the purification may be repeated a plurality of times. Good. Moreover, you may perform a deodorizing process. As a specific treatment method, there is a deodorization treatment by combined use of water washing, urease, and heating.

  The molecular weight of the extract obtained from the sturgeon float bag is preferably 100 to 300,000, more preferably 500 to 50,000, and particularly preferably 1,000 to 10,000. If the weight average molecular weight is too large, it may be difficult to dissolve in a solvent such as water, or the cosmetic containing the extract may lack stability, while if it is too small, In some cases, the effect of the cosmetic of the present invention cannot be obtained.

  Extracts from sturgeon floats have little fish-specific odors compared to extracts from seawater fish such as kochi, cod, flounder, salmon, butterfly, sardine, tuna, and sea bream. The content can be increased and can be used widely and suitably for various cosmetics.

  The obtained extract from the sturgeon float bag may be adjusted to pH 4 to 8 and used as it is or after further concentration. If necessary, it can be pulverized according to conventional methods such as spray drying and freeze drying. May be.

  The method for lyophilization is not particularly limited, and the volume is not changed by generally known methods and the conditions thereof, that is, by freezing at low temperature and then drying under high vacuum. What is necessary is just to employ | adopt the method and its conditions which freeze-separate and evaporate (sublimate) solvent components, such as a water | moisture content, and obtain what has a rapid condensate while having a pore. Further, a desired pretreatment may be appropriately performed before actual freeze-drying. Specifically, for example, an extract from a sturgeon float or a solution thereof is dispensed into a glass bottle or the like and then freeze-dried, or a solution of the extract from a sturgeon float is placed in liquid nitrogen. Examples include a method of freeze-drying a water droplet shape (particle shape, bead shape) by addition (dropping). Various processing conditions such as temperature, pressure, and time relating to lyophilization can be performed under the same conditions as those of a commonly known lyophilization method.

  When the extract from the sturgeon float obtained by the above method is pulverized, the particle size is preferably 5 mm or less, more preferably 45 μm to 2 mm, and still more preferably 75 μm to 500 μm. If the particle size is too large, the effect of improving re-solubility may be difficult to recognize, and if it is too small, handling may be difficult.

  The blending amount of the extract from the sturgeon float bag is usually 0.0001% by mass to 10% by mass, preferably 0.001% by mass to 1% by mass in terms of solid content (in terms of dry matter) with respect to the entire cosmetic. It is. Most preferably, it is 0.01 mass%-0.1 mass%. When the content is too small, the effect of the present invention is not observed, and when it is too large, an increase in the effect commensurate with the blending amount may not be expected.

  According to dosage form, in the case of basic cosmetics, it is usually 0.0005% by mass to 1% by mass, preferably 0.005% by mass to 0.1% by mass, and particularly preferably 0.05% by mass to 0.1%. % By mass. In the case of makeup cosmetics, it is usually 0.0001% by mass to 1% by mass, preferably 0.0005% by mass to 0.05% by mass, particularly preferably 0.001% by mass to 0.01% by mass. is there. In the case of hair care cosmetics, it is usually 0.0005% by mass to 1% by mass, preferably 0.005% by mass to 0.1% by mass, particularly preferably 0.05% by mass to 0.1% by mass. .

  It is also preferable for the cosmetic of the present invention to contain an extract from a floating bag of fish other than sturgeon from the viewpoint of cost reduction and securing / saving of sturgeon resources. Examples of fish other than sturgeon include saltwater fish such as kochi, cod, flounder, salmon, kingfisher, sardine, tuna, and sea bream; and freshwater fish such as carp and crucian carp.

  The content of the extract from the float of fish other than sturgeon is not particularly limited, but is preferably 70% by mass or less, more preferably 40% by mass or less, in terms of solid content, with respect to the entire extract from the float. 20 mass% or less is especially preferable, and it is most preferable not to contain the extract from the floating bag of fishes other than sturgeon. When the content of the extract from the floating bag of fish other than sturgeon is too large, the above-described effects of the present invention may not be obtained.

  The method for obtaining the extract from the floating bag of fish other than sturgeon can be carried out according to the method for obtaining the extract from the above-described bag of sturgeon. In addition, an extract from Aisin Glass obtained by processing a floating bag of fish other than sturgeon can also be suitably used in the present invention. In other words, the cosmetic is preferably the above-mentioned “extract from the sturgeon float bag” and / or “extract from the float bag of fish other than the sturgeon” is an extract from Aisin glass formed by processing the float bag.

  The cosmetics of the present invention include basic cosmetics such as face wash, lotion, cosmetic liquid, pack, milky lotion, cream, mask sheet, etc .; makeup cosmetics such as base cream, foundation, lipstick, eye shadow, cheek red ; Toiletry cosmetics such as shampoos, rinses, treatments, hair packs, soaps, body soaps, bathing agents; hair care cosmetics such as hair tonics, hair liquids, hair creams, etc.

  Examples of the dosage form of the cosmetic of the present invention include gels, packs, liposomes, liquids, clays, solid powders, aerosols, and haptics. Moreover, as a usage method, various methods, such as an aerosol type | formula, a pump type | formula, an extrusion type | formula, the system injected by these, and a bath agent, are mentioned.

  The cosmetic of the present invention can contain “other ingredients” in accordance with the dosage form in addition to the extract from the sturgeon float. Specific examples of the “other components” include water, alcohol, surfactant (cation, anion, nonion, amphoteric surfactant, etc.), humectant (glycerin, 1,3-butylene glycol, propylene glycol, Dipropylene glycol, amino acids, urea, pyrrolidone carboxylates, nucleic acids, monosaccharides, oligosaccharides and their derivatives, etc., thickeners (polysaccharides, polyacrylates, carboxyvinyl polymers, polyvinylpyrrolidone, polyvinyl alcohol, Chitin, chitosan, alginic acid, carrageenan, xanthan gum, methylcellulose and their derivatives), wax, petrolatum, hydrocarbon saturated fatty acid, unsaturated fatty acid, silicone oil and their derivatives, tri (caprylic and capric acid) glyceryl, trioctane Triglycerides such as glyceryl acid Rides, ester oils such as isopropyl stearate, natural fats and oils (Olive oil, camellia oil, avocado oil, almond oil, cocoa butter, evening primrose oil, grape seed oil, macadamia nut oil, eucalyptus oil, rosehip oil, squalane , Orange luffy oil, lanolin, ceramide, etc.), preservatives (oxybenzoic acid derivatives, dehydroacetates, photosensitizers, sorbic acid, phenoxyethanol, etc. and their derivatives), bactericides (sulfur, triclocarbanilide, salicylic acid, zinc pyrithione) , Hinokitiol, etc. and their derivatives), UV absorbers (paraaminobenzoic acid, methoxycinnamic acid, etc. and their derivatives), anti-inflammatory agents (allantoin, crityllithic acid, etc. and their derivatives, etc.), antioxidants ( Tocopherol, BHA, BHT etc. and their Conductors, etc.), chelating agents (ededic acid, hydroxyethanediphosphonic acid, etc. and derivatives thereof), animal and plant extracts (Ashitaba, aloe, Ages, Ogon, grasshopper, seaweed, Karin, Chamomile, licorice, kiwi, cucumber, mulberry, Birch, Toki, Garlic, Button, Hops, Maronier, Lavender, Rosemary, Eucalyptus, Milk, Various Peptides, Placenta, Royal Jelly, etc. and their containing components, purified products or fermented products, etc., pH adjusters (inorganic acids, inorganic acids Salt, organic acid, organic acid salt, etc. and their derivatives), vitamins (vitamin A, vitamin B, vitamin C, vitamin D, etc. and their derivatives), titanium oxide, talc, mica, silica , Zinc oxide, iron oxide, silicon, and powders processed from these to achieve the object of the present invention In a range of 0.001 to 99.9% by mass. In addition, the component which comprises the cosmetics of this invention is by no means limited to the above-mentioned, and if it is a component used for cosmetics, it can select freely.

  In the haptic agent, in addition to the above components, a base (such as kaolin and bentonite) and a gelling agent (such as polyacrylate and polyvinyl alcohol) can be blended. Moreover, in a bath agent, a sulfate, bicarbonate, borate, a pigment | dye, and a moisturizing agent are mix | blended suitably, and it can adjust to a powder type and a liquid agent type.

  Hereinafter, the present invention will be described more specifically with reference to Examples and Comparative Examples, but the present invention is not limited to these unless it exceeds the gist. Hereinafter, unless otherwise specified, “%” means “mass%”.

Preparation Example 1
Using 1 kg of a vestel sturgeon float bag, it was immersed in a mixed solvent of water and 1,3-butylene glycol (mass ratio 7: 3) at 20 ° C. for 1 week, followed by solvent extraction to obtain an extract solution. After the residue was filtered off, the solution in which the extract was dissolved was purified by filtration to obtain 90 kg of the extract (A) from the sturgeon bag. The following evaluation was performed using this.

Preparation Example 2
1 kg of a bastel sturgeon float bag was immersed in 33 kg of water, 0.05% by mass of trypsin was added to the float bag, and reacted at 37 ° C. for 4 hours. Subsequently, heat treatment at 85 ° C. for 30 minutes was performed to deactivate the trypsin. The solution was cooled and purified by filtration to obtain 30 kg of an extract (B) from a sturgeon float bag. The following evaluation was performed using this.

<Evaluation 1: Moisturizing effect of the extract>
1 mL each of the extract (B) extracted from the sturgeon float bag prepared in Preparation Example 2 was applied to the inside of the forearms of five subjects twice a day in the morning and in the evening for 30 days. After the 30-day rubbing period, the stratum corneum moisture content of the rubbing part of each subject was measured. Here, the amount of horny water is a value when measured according to a conventional method using a Corneometer (trade name) manufactured by Integral Co.

  As can be seen from the results in Table 1, when the extract (B) prepared in Preparation Example 2 was used, the amount of stratum corneum increased as compared with the case where the solvent was simply used.

<Evaluation 2: Collagen synthesis effect>
As a basic medium, Eagle MEM medium containing 1% fetal calf serum was used, and human dermis-derived fibroblasts were used as cells. After culturing the cells in a basic medium for 24 hours, a medium containing 1 to 5% of the extract (B) from the sturgeon float bag prepared in Preparation Example 2 was added and cultured for 2 days. Culture was performed using only the basic medium as a blank. At this time, collagen was quantified using a collagen stain kit manufactured by Cosmo Bio.

  The results are shown in FIG. Those containing Extract B had an increased amount of protein, especially an increased amount of collagen. About this effect, content dependency of the extract B content 0%, 1%, 2%, 5% was confirmed.

Example 1
(Lotion)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Extract (A) 1.00
Concentrated glycerin 2.00
1,3-butylene glycol 10.00
Xanthan gum 0.05
Sodium hyaluronate 0.01
Preservative Appropriate amount of purified water Amount that makes the total amount 100.00

Example 2
(Milky lotion)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Extract (A) 3.00
Glyceryl trioctanoate 3.00
Jojoba oil 1.00
Olive oil 1.00
Methylpolysiloxane 0.10
Natural vitamin E 0.10
POE (20) sorbitan monooleate 1.50
Sorbitan monooleate 1.50
L-Arginine 2.00
L-serine 0.50
Sodium pyrrolidonecarboxylate 0.10
Polyvinylpyrrolidone 0.05
Preservative Appropriate amount of purified water Amount that makes the total amount 100.00

Example 3
(cream)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Extract (B) 0.50
Behenyl alcohol 2.00
Glyceryl trioctanoate 3.00
Jojoba oil 1.00
Olive oil 1.00
Methylpolysiloxane 0.10
Natural vitamin E 0.10
POE (20) sorbitan monostearate 2.50
POE (80) hydrogenated castor oil 0.50
L-Arginine 2.00
L-serine 0.50
Sodium polypyrrolidonecarboxylate 0.10
Carboxyvinyl polymer 0.05
Potassium hydroxide 0.01
Preservative Appropriate amount of purified water Amount that makes the total amount 100.00

Example 4
(Cosmetic liquid)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Extract (A) 5.00
Carboxyvinyl polymer (2% aqueous solution) 10.00
Carboxyvinylcellulose 3.00
Glycerin 10.00
Plant extract 0.50
Triethanolamine 0.15
Methylparaben 0.10
Quinn seed solution (1% aqueous solution) 10.00
Sodium hyaluronate (1% aqueous solution) 4.00
L-Arginine 2.00
Purified water Amount that makes the total amount 100.00

Example 5
(pack)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Extract (B) 1.50
Hydroxyethoxycellulose 3.00
Carboxyvinyl polymer (2% aqueous solution) 10.00
Polyoxyethylene oleyl ether (15EO) 1.00
L-Arginine 2.00
Ethanol 5.00
Preservative Appropriate amount of purified water Amount that makes the total amount 100.00

Example 6
(Bath)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Extract (B) 0.10
Anhydrous sodium sulfate 45.00
Sodium bicarbonate 50.00
Sodium borate 3.00
L-Arginine 2.00

Comparative Example 1
(Comparative lotion)
In Example 1, a comparative lotion was prepared in the same manner as in Example 1 except that purified water was used instead of the extract (A).

Comparative Example 2
(Comparative emulsion)
In Example 2, a comparative emulsion was prepared in the same manner as in Example 2 except that purified water was used instead of the extract (A).

Comparative Example 3
(Comparison cream)
In Example 3, a comparative cream was prepared in the same manner as in Example 3, except that purified water was used instead of the extract (B).

Comparative Example 4
(Comparative serum)
In Example 4, a comparative cosmetic liquid was prepared in the same manner as in Example 4 except that purified water was used instead of the extract (A).

Comparative Example 5
(Comparison pack)
In Example 5, a comparative pack was prepared in the same manner as in Example 5 except that purified water was used instead of the extract (B).

Comparative Example 6
(Comparison bath)
A comparative bath preparation was prepared in the same manner as in Example 6 except that sodium bicarbonate was used in place of the extract (B) of Example 6.

<Evaluation 3: Moisturizing effect as cosmetics>
The skin lotion obtained in Example 1 and the skin lotion obtained in Comparative Example 1 were applied to the inner left and right forearms of 20 female subjects, 1 mL each, for 30 days every day in the morning and evening. Measured and compared. The results are shown in Table 2. Here, the stratum corneum moisture content is a value when measured according to a conventional method using a Corneometer (trade name) manufactured by Integral Co.

  As can be seen from the results in Table 2, when “the lotion containing the extract (A)” prepared in Example 1 was used, the “comparative lotion not containing the extract (A)” prepared in Comparative Example 1 The amount of keratin moisture increased compared with the case of using.

<Evaluation 4: Skin condition improvement effect>
For 10 female subjects aged 40-50 years who complained of skin deterioration, the cosmetics of Examples 1 to 5 and Comparative cosmetics of Comparative Examples 1 to 5 were applied twice in the morning and evening on the entire face. For the bath agent of Example 6 and the comparative bath agent of Comparative Example 6, 50 grams of the bath agent was dissolved in 200 L of hot water and used once a day, based on the following criteria. With regard to (a) moist feeling of skin, (b) skin gloss, and (c) skin firmness before and after use, a sensory evaluation was performed one month after the start of use. None of the patients complained of skin abnormalities throughout the study period.

[Skin condition improvement criteria]
A Extremely improved B Significantly improved C Slightly improved D No change E Feeled worse

With respect to “(a) moist feeling of skin”, Table 3 shows the number of persons determined as determinations A to E.

With respect to “(b) skin gloss”, Table 4 shows the number of persons who are judged as A to E.

With respect to “(c) skin firmness”, Table 5 shows the number of persons who are judged as A to E.

  From the results of Tables 3 to 5, the skin condition improving action is compared with the cosmetics of the examples in all of (a) moist feeling of skin, (b) gloss of skin, and (c) firmness of skin. It was much better than the cosmetics of the example.

Example 7
(Hair cream)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Liquid paraffin 15.00
Vaseline 15.00
Beeslow 2.00
Preservative appropriate amount extract (A) 5.00
Carboxyvinyl polymer 0.10
Xanthan gum 0.10
Glycerin 5.00
Polyoxyethylene hydrogenated castor oil 3.00
Caustic soda 0.05
Purified water Amount that makes the total amount 100.00

Comparative Example 7
(Comparison hair cream)
A comparative hair cream was prepared in the same manner as in Example 7 except that purified water was used instead of the extract (A).

Example 8
(rinse)
The following blending components were blended and prepared according to a conventional method.
[Ingredients] [mass%]
Extract (A) 5.00
Silicone oil 3.00
Liquid paraffin 1.00
Cetyl alcohol 1.50
Stearyl alcohol 1.00
Stearyltrimethylammonium chloride 0.70
Glycerin 3.00
Preservative Appropriate amount of purified water Amount that makes the total amount 100.00

Comparative Example 8
(Comparison rinse)
A comparative rinse was prepared in the same manner as in Example 7 except that purified water was used instead of the extract (A).

<Evaluation 5: Hair condition improvement effect>
Randomly selected 20 female subjects aged between 20 and 40 years old, the cosmetics of Examples 7 and 8 and the comparative cosmetics of Comparative Examples 7 and 8 were used daily on the hair. Based on these criteria, a sensory evaluation was performed one month after the start of use of the hair condition before and after use for (a) combing and (b) hair gloss. None of the patients complained of abnormalities throughout the study period.

[Hair condition improvement criteria]
A Extremely improved B Significantly improved C Slightly improved D No change E Feeled worse

With respect to “(a) comb street”, the number of persons determined as A to E is shown in the table.

With respect to “(b) Hair gloss”, the number of each of the determinations A to E is shown in the table.

  In Production Example 1 and Production Example 2, when the extract is obtained after being processed into Aisin glass once, in the evaluations 1 to 5, the cosmetics of the examples are remarkably superior. Similar results were obtained.

  The cosmetic of the present invention prevents skin damage, realizes a youthful and healthy skin condition, and has a skin-beautifying effect, so it is the basis for soap, face wash, lotion, beauty liquid, milky lotion, cream, pack, etc. It is widely used for cosmetics, shampoos, rinses, hair cosmetics such as hair conditioners, makeup cosmetics such as lipsticks, foundation creams and foundations, toiletries such as soaps, body soaps and bath additives.

It is a figure which shows the collagen synthesis effect by the extract from the sturgeon float bag using the human dermis origin fibroblast (evaluation 2).

Claims (10)

  A cosmetic comprising an extract from a sturgeon float.   The cosmetic according to claim 1, wherein the extract from the sturgeon float is extracted with water.   The cosmetic according to claim 1, wherein the extract from the sturgeon bag is extracted with a hydrophilic organic solvent or a mixed solvent of water and a hydrophilic organic solvent.   The cosmetic according to claim 3, wherein the hydrophilic organic solvent is 1,3-butylene glycol, 1,2-hexanediol or pentanediol.   5. The extract according to any one of claims 1 to 4, wherein the extract from the sturgeon float bag is extracted after hydrolysis or hydrolysis of collagen contained in the sturgeon float bag. Cosmetics.   The cosmetic according to claim 5, wherein the hydrolysis is performed by a proteolytic enzyme.   Furthermore, the cosmetics in any one of the Claims 1 thru | or 6 containing the extract from the floating bag of fishes other than sturgeon.   The extract according to any one of claims 1 to 7, wherein the extract from the sturgeon float bag and / or the extract from the fish float bag other than the sturgeon is an extract from Aisin glass obtained by processing a float bag. Cosmetics according to item.   The cosmetic according to any one of claims 1 to 8, wherein the sturgeon is an Amur sturgeon, a white sturgeon, a Siberian sturgeon, a sturgeon or a vestel sturgeon.   An extract from a sturgeon float bag for cosmetics, which is contained in the cosmetic according to any one of claims 1 to 9.

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