JP2009215549A - Allergen deactivating composition and method for deactivating allergen - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an allergen deactivating composition having an excellent deactivation effect to an allergen, and to provide a simple method for deactivating an allergen without requiring a treatment such as heating. <P>SOLUTION: The allergen deactivating composition includes: (A) a heavy metal compound selected from a group including a water-soluble compound containing a heavy metal and a water-dispersible compound containing a heavy metal; and (B) an inorganic peroxide which generates hydrogen peroxide by dissolving into water; wherein the diluted liquid prepared by diluting with distilled water to 10,000 times by mass standard has a pH value of 7.0-11.0 at 25°C. The method of deactivating allergen using the allergen deactivating composition is also provided. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention particularly relates to an allergen inactivating agent composition and an allergen inactivating method for inactivating allergens released from living organisms, allergens derived from dead bodies of living organisms, and the like.

The development of techniques for inactivating antigenic substances (allergens) that cause allergies has been carried out so far.
As components for inactivating allergens (allergen inactivating components), for example, tannic acid, colloidal silica, and the like are known.

Patent Document 1 proposes an allergen inactivating agent composition containing an effective amount of allergen neutralizing metal ions and a solvent.
As the metal ions, zinc ions and tin ions are listed as preferred. In the examples, compositions (solutions) containing zinc chloride and adjusted to an acidity having a pH of about 3.6 are disclosed. Yes.
An example of a composition (solution) containing tannic acid as an allergen inactivating component is also disclosed.

  Moreover, the heat processing method is also mentioned as a method of inactivating an allergen.

Japanese translation of PCT publication No. 2004-510717

However, when tannic acid is used as the allergen inactivating component as in the allergen inactivating agent composition described in Patent Document 1, the object to be treated is colored by applying a composition containing tannic acid. There is a problem that is easy to do. Moreover, when colloidal silica is used as an allergen deactivating component, there is a problem that a composition containing colloidal silica is easily solidified.
Furthermore, in the conventional allergen inactivating agent composition containing allergen inactivating components such as tannic acid and colloidal silica, the allergen inactivating effect is not yet sufficient, and the higher allergen inactivating effect is achieved. Development of technology to demonstrate is required.
Further, the above heat treatment method is complicated, and a simpler method is desired.

  The present invention has been made in view of the above circumstances, and provides an allergen inactivating agent composition excellent in the effect of allergen inactivation, and a simple allergen inactivating method without requiring heat treatment or the like. This is the issue.

As a result of intensive studies, the present inventor provides the following means in order to solve the above problems.
That is, the allergen inactivating agent composition of the present invention comprises a heavy metal compound (A) selected from the group consisting of a water-soluble compound containing a heavy metal and a water-dispersible compound containing a heavy metal, and an aqueous solution dissolved in water. The pH at 25 ° C. of a diluted solution containing an inorganic peroxide (B) that generates hydrogen oxide and diluted 10000 times on a mass basis with distilled water is 7.0 to 11.0. Features.

The allergen inactivating agent composition of the present invention preferably further contains an organic peracid precursor (C).
In the allergen inactivating agent composition of the present invention, the heavy metal compound (A) is at least one water-soluble salt selected from the group consisting of a water-soluble salt containing copper and a water-soluble salt containing iron. It is preferable that
In addition, the allergen inactivating agent composition of the present invention is suitable for inactivating allergens released from living organisms or allergens derived from dead bodies of living organisms.

  The allergen inactivation method of the present invention is a method of using the allergen inactivating agent composition of the present invention.

  ADVANTAGE OF THE INVENTION According to this invention, the allergen inactivation agent composition excellent in the effect of allergen inactivation, and the simple allergen inactivation method without requiring heat processing etc. can be provided.

≪Allergen deactivator composition≫
The allergen deactivator composition of the present invention comprises a heavy metal compound (A) selected from the group consisting of a water-soluble compound containing heavy metal and a water-dispersible compound containing heavy metal, and hydrogen peroxide dissolved in water. The pH at 25 ° C. of a diluted solution containing an inorganic peroxide (B) that generates water and diluted 10000 times on a mass basis with distilled water is 7.0 to 11.0.
The allergen inactivating agent composition of the present invention preferably further contains an organic peracid precursor (C).
The form of the allergen inactivating agent composition is not particularly limited, and may be, for example, a powdery or granular composition or a liquid composition. Especially, since the higher effect of allergen inactivation is easy to be acquired, it is preferable that it is a powdery or granular composition. Unlike the liquid composition, the powdered or granular composition does not generate hydrogen peroxide by dissolving the inorganic peroxide (B) in water. Accordingly, there is no possibility that the effect of allergen inactivation is reduced or the stability of the composition is deteriorated, which is a preferable form.

[(A) component]
In the present invention, the heavy metal compound (A) (hereinafter referred to as “component (A)”) is selected from the group consisting of water-soluble compounds containing heavy metals and water-dispersible compounds containing heavy metals.
Examples of the water-dispersible compound containing a heavy metal include those that disperse in a solvent as colloidal fine particles when dissolved in a solvent such as water.
This (A) component can inactivate allergens well by using in combination with the inorganic peroxide (B) described later. Also, the uniformity when dissolved in a solvent such as water is good.
Especially, as (A) component, since the solubility to solvents, such as water, is further favorable, it is preferable that it is a water-soluble compound containing a heavy metal.

As the component (A), a compound containing a heavy metal such as iron, lead, gold, platinum, silver, copper, chromium, cadmium, mercury, zinc, arsenic, manganese, cobalt, nickel, molybdenum, tungsten, tin or bismuth, or The compound which discharge | releases these metal ions is mentioned.
Among them, a compound containing a transition element selected from the group consisting of iron, silver, copper, manganese, cobalt, nickel and molybdenum, or a compound which releases these metal ions; selected from the group consisting of zinc, tin and bismuth A compound containing a typical element or a compound that releases these metal ions is preferable.
Among them, the compound containing the transition element or the compound releasing these metal ions is more preferable; the compound containing the transition element of the fourth period in the long-period periodic table, or the compound releasing these metal ions. More preferably; compounds containing iron or copper, or compounds releasing these metal ions are particularly preferred; compounds containing copper or compounds releasing copper ions are most preferred.
Specific examples of the component (A) include salts, oxides, hydroxides, sulfides, metal colloids, or organometallic compounds containing the above heavy metals. Especially, the salt containing the said heavy metal is preferable, and the water-soluble salt containing the said heavy metal is especially preferable.
Salts containing heavy metals include halides such as chlorides, acetates, nitrates, carbonates, sulfates, phosphates, perchlorates and other inorganic salts; benzoates, citrates, oleates And organic acid salts such as caprylate, caprate, laurate, myristate, palmitate, stearate, oxalate, and tannic acid. Of these, halides, nitrates, sulfates, acetates and citrates are preferable, chlorides, nitrates and sulfates are more preferable, and chlorides and sulfates are more preferable.
As the component (A), a hydrate of the above compound can be used in addition to the above compound.

(A) A component can be used individually by 1 type or in combination of 2 or more types as appropriate.
In the present invention, the component (A) is preferably at least one heavy metal compound selected from the group consisting of a water-soluble compound containing copper or iron and a water-dispersible compound containing copper or iron, such as water. At least one water-soluble salt selected from the group consisting of a water-soluble salt containing copper and a water-soluble salt containing iron because the solubility in a solvent is good and the allergen inactivation effect is excellent. The water-soluble salt containing copper is particularly preferable.

The content ratio of the component (A) in the allergen deactivator composition of the present invention (when the component (A) is used as an anhydride) is determined by dissolving and diluting the allergen deactivator composition in water or the like. When used, it is preferably 0.0006 to 7% by mass, more preferably 0.006 to 0.7% by mass, and the allergen deactivator composition is a powdery or granular composition When it is, it is especially preferable that it is 0.06-0.7 mass%.
When the content ratio of the component (A) is not less than the lower limit of the above range, the effect of allergen inactivation is further improved. (A) When the content rate of a component is below the upper limit of the said range, the mixing | blending balance with another component can be taken.

In addition, the content ratio of the component (A) in the allergen deactivator composition of the present invention (when the component (A) is used as a hydrate) is diluted by dissolving the allergen deactivator composition in water or the like. When used, it is preferably 0.001 to 10% by mass, more preferably 0.01 to 1% by mass, and the allergen deactivator composition is a powdery or granular composition When it is, it is especially preferable that it is 0.1-1 mass%.
When the content ratio of the component (A) is not less than the lower limit of the above range, the effect of allergen inactivation is further improved. (A) When the content rate of a component is below the upper limit of the said range, the mixing | blending balance with another component can be taken.

[Component (B)]
The inorganic peroxide (B) in the present invention (hereinafter referred to as “component (B)”) is dissolved in water to generate hydrogen peroxide.
Such component (B) can inactivate allergens well in combination with component (A).
As the component (B), any component that generates hydrogen peroxide in an aqueous solution may be used. For example, percarbonate, perboric acid, or alkali metal salts (such as sodium salt and potassium salt) or ammonium salts thereof may be used. Can be mentioned. Especially, since it is excellent in the effect of allergen inactivation, it is preferable that they are sodium percarbonate and sodium perborate, and it is more preferable that it is sodium percarbonate.

  (B) A component can be used individually by 1 type or in combination of 2 or more types as appropriate.

The content ratio of the component (B) in the allergen inactivating agent composition of the present invention is 1 to 90% by mass when the allergen inactivating agent composition is dissolved in water or the like and diluted. Preferably, when the allergen deactivator composition is a powdery or granular composition, it is more preferably 5 to 90% by mass, and particularly preferably 20 to 50% by mass.
(B) When the content rate of a component is more than the lower limit of the said range, the effect of allergen inactivation improves more. (B) When the content rate of a component is below the upper limit of the said range, the mixing | blending balance with another component can be taken.

[Component (C)]
In the present invention, in addition to the component (A) and the component (B), an organic peracid precursor (C) (hereinafter referred to as “component (C)”) is further contained to inactivate allergens. The effect of further improves.
The component (C) is not particularly limited as long as it generates an organic peracid by perhydrolysis. For example, it can be suitably used from those conventionally proposed as a bleach activator.
Here, “organic peracid” means a compound in which an —OH (hydroxyl group) -containing acidic group (carboxy group, sulfo group, etc.) is substituted with —OOH, for example, Examples thereof include percarboxylic acid having —C (═O) OOH (percarboxy group), peroxosulfonic acid having —S (═O) ₂ OOH, and the like.

  Specific examples of the component (C) include alkyl acyloxybenzene sulfonate (OBS) and alkyl acyloxybenzoic acid (OBC).

Preferred examples of the alkyl acyloxybenzene sulfonate (OBS) and the alkyl acyloxybenzoic acid (OBC) include compounds represented by the following general formula (I) or the following general formula (II).
_{RCOO-C 6 H 4 -COOM ···} (I)
_{RCOO-C 6 H 4 -SO 3} M ··· (II)
[In the formulas (I) and (II), each R independently represents a linear or branched alkyl group or alkenyl group having 2 to 14 carbon atoms, and each M independently represents a hydrogen atom or a salt. Represents the forming cation. ]

In the formulas (I) and (II), R represents a linear or branched alkyl group or alkenyl group having 2 to 14 carbon atoms. In R, as for carbon number, 8-12 are preferable and 10-12 are more preferable.
In the formulas (I) and (II), M represents a hydrogen atom or a salt-forming cation. Examples of the salt-forming cation include alkali metal ions such as sodium and potassium; ammonium ions, secondary amine ions such as diethanolamine, and tertiary amine ions such as triethanolamine.
The compound represented by the general formula (I) or the general formula (II) is an optionally substituted perbenzoic acid or an aliphatic peroxocarboxylic acid (preferably 1 to 14 carboxylic acids) under the conditions of perhydrolysis. A carbon atom, more preferably 2 to 12 carbon atoms.).

In addition to the above OBS and OBC, the component (C) includes, for example, polyacylated alkylenediamine such as tetraacetylethylenediamine (TAED) or a salt thereof; 1,5-diacetyl-2,4-dioxohexahydro Acylated triazine derivatives such as 1,3,5-triazine (DADHT); acylated glycoluril such as 1,3,4,6-tetraacetylglycoluril (TAGU); N such as N-nonanoylsuccinimide (NOSI) -Acylimide; acylated hydrocarboxylic acid such as triethyl-O-acetyl citrate (TEOC); carboxylic acid anhydride such as phthalic anhydride, isatoic anhydride, succinic anhydride; N-methyldiacetamide, glycolide, etc. Carboxylic acid amides; triacetin, ethylene glycol diacetate, Propenyl acetate, 2,5-diacetoxy-2,5-dihydrofuran or enol ester (known from DE 196 16 673 and DE 196 16 767), acetylated sorbitol, mannitol or these Acylated polyhydric alcohols such as mixtures (SORMAN) (described in EP 0 525 239); pentaacetyl glucose (PAG), pentaacetyl fructose, tetraacetyl xylose, octaacetyl lactose, or acetylated, optionally Acylated sugar derivatives such as N-alkylated glucamine or gluconolactone; triazole or triazole derivatives; granular caprolactam or caprolactam derivatives [preferably -Benzoylcaprolactam or N-acetylcaprolactam (these are WO 94/27970, WO 94/28102, WO 94/28103, WO 95/00626, WO No. 95/14759 and WO 95/17498, etc.)).
Also substituted hydrophilic acyl acetals (known from German Offenlegungsschrift DE-A-19616769), acyllactams (German Offenlegungsschrift DE-A-19616770 and international). Also described in the publication No. 95/14075 pamphlet).
It is also possible to use a combination of usual organic peracid precursors known from DE-A-4443177.
Furthermore, nitrile derivatives such as cyanopyridine, N-alkylammonium acetonitrile, and nitrile quat; cyanamide derivatives and N-methylmorpholinium acetonitrile (MMA) can also be used.
Also, benzoyloxybenzene sulfonate such as Benzoic acid 4-sulfo (1-4-sulfophenyl) ester can be used.

(C) A component can be used individually by 1 type or in combination of 2 or more types as appropriate.
In the present invention, the component (C) generates an organic peracid having an alkyl group having 2 to 12 carbon atoms or an alkenyl group having 2 to 12 carbon atoms because the effect of allergen inactivation is particularly improved. Is preferred. The alkyl group or alkenyl group preferably has 8 to 12 carbon atoms, and more preferably 10 to 12 carbon atoms. Among them, OBS or OBC in which R in the general formula (I) or the general formula (II) has 8 to 12 carbon atoms is more preferable, and R in the general formula (I) or the general formula (II) is more preferable. OBS or OBC having 10 to 12 carbon atoms is particularly preferable.

The content ratio of the component (C) in the allergen inactivating agent composition of the present invention is 0.05 to 10% by mass when the allergen inactivating agent composition is dissolved in water or the like and diluted. It is preferable. In particular, when the allergen deactivator composition is a powdery or granular composition, it is preferably 0.05 to 10% by mass, more preferably 0.1 to 5% by mass, It is especially preferable that it is 0.1-3 mass%.
When the content ratio of the component (C) is not less than the lower limit of the above range, the effect of allergen inactivation is further improved. (C) When the content rate of a component is below the upper limit of the said range, the mixing | blending balance with another component can be taken.

[Other ingredients]
In the allergen inactivating agent composition of the present invention, other components other than the components (A), (B), and (C) may be used in combination as necessary.
Examples of other components include alkali agents such as sodium carbonate, potassium carbonate, and sodium silicate; organic acids such as citric acid and malic acid; inorganic acids such as phosphoric acid, surfactants, and chelating agents.
Among these, as other components, an alkali agent, an organic acid, and an inorganic acid are preferably used for the purpose of easily adjusting the pH of the diluted treatment liquid described later within the range of 7.0 to 11.0. However, an alkali agent is more preferable, and sodium carbonate is particularly preferable.

  The preparation method of the allergen inactivation agent composition of this invention is not specifically limited, It can prepare by mixing each component according to a conventional method.

(Diluted solution)
In the allergen inactivating agent composition of the present invention, the pH at 25 ° C. of the diluted treatment solution diluted 10,000 times by mass with distilled water is 7.0 to 11.0, and pH 9.0 to 11.0. It is preferable that the pH is 10.0 to 11.0. When the pH is 7.0 or higher, the effect of allergen inactivation is excellent. When the pH is 11.0 or less, a weakly alkaline region can be satisfied (in addition, if the pH exceeds 11.0, it becomes alkaline in the household goods quality labeling method. Moreover, the object to be treated is altered. Etc.).
In the present invention, the pH of the dilution treatment liquid is adjusted to JIS K3362-1998 by adjusting the dilution treatment liquid to 25 ° C. and using a glass electrode type pH meter (product name: F-52, manufactured by Horiba, Ltd.). According to the standard, the pH value indicated after one minute has passed after the glass electrode is immersed in the diluted solution.

A method for preparing such a diluted treatment liquid is not particularly limited, and a method of dissolving an allergen deactivator composition in distilled water and diluting to a predetermined dilution ratio, or an allergen deactivator composition The method etc. which melt | dissolve in distilled water and dilute several times and dilute to predetermined dilution rate etc. are mentioned.
Specifically, as a method for preparing the diluted processing solution, for example, a powdered or granular allergen deactivator composition is dissolved in distilled water, and the diluted processing solution is prepared by performing multiple dilutions. It is preferable to do.
Especially, (A) component and (B) component (preferably (C) component) in the said composition are contained more uniformly with respect to a powdery or granular allergen deactivator composition. It is more preferable to prepare a diluted solution by dissolving the fraction-treated product as described above in distilled water and diluting it at a predetermined magnification.

  In the allergen deactivator composition of the present invention, as a method for controlling the pH of the diluted treatment liquid at 25 ° C. to 7.0 to 11.0, for example, the change of the hydrogen ion concentration in the aqueous solution of pH 7 to 13 is exemplified. A method of using a pH buffering agent (for example, sodium carbonate, sodium silicate, potassium carbonate, sodium borate; an organic acid such as citric acid, malic acid, etc.) having a reducing action alone or in combination of two or more. In the case of a body-like or granular composition, a method of adjusting the blending amount of the alkaline agent can be mentioned.

In the allergen inactivating agent composition of the present invention, the allergen to be inactivated is not particularly limited, and the allergen released from the organism, the allergen derived from the dead body of the organism, and the organism itself to humans. And allergens that enter and release.
Among them, the allergen inactivating agent composition of the present invention is particularly suitable for inactivating allergens released from living organisms or allergens derived from dead bodies of living organisms.
Specific examples of allergens released from living organisms include allergens attached to pet hair, pollen, mite feces, and mold spores.
Specific examples of allergens derived from living carcasses include tick carcasses and cockroach carcasses.

  The method for using the allergen inactivating agent composition of the present invention is not particularly limited. For example, the method described in <Allergen inactivating method> described below is simple and does not require heat treatment, This is a particularly suitable method.

≪Allergen inactivation method≫
The allergen inactivation method of the present invention is a method of using the allergen inactivating agent composition of the present invention.
Specifically, for example, a method in which the allergen inactivating agent composition of the present invention or a diluted solution thereof is directly applied to a target allergen present in a spray container or the like and applied by spraying or the like. Is mentioned.
In addition, by spraying the allergen deactivator composition of the present invention or its diluted solution from a spray container or the like toward a living space such as a living space or a vehicle space such as a car, the inside of the living space And a method for inactivating allergens floating in the water.
What is necessary is just to prepare the dilution process liquid used when performing the allergen inactivation method similarly to the "preparation method of (dilution process liquid)" mentioned above. In that case, any of ion-exchange water, distilled water, industrial water, tap water, etc. can be used for the water used for dilution of the allergen deactivator composition.

The method of applying the allergen deactivator composition or the diluted treatment liquid to the inactive target allergen is not particularly limited. The method of spraying from a spray container, Preferable examples include a method of applying using various known containers, such as a method of applying the container by bringing the application part of the container into direct contact with the existing one.
In addition, allergens to be inactivated include, for example, daily life products, that is, textile products such as sofas, carpets, curtains, cushions, bedding; tatami mats, floors, walls, windows, furniture, toys Products in the living space such as stuffed animals; products in the vehicle space such as car seats.

As described above, according to the present invention, it is possible to provide an allergen inactivating agent composition excellent in the effect of allergen inactivation, and a simple allergen inactivating method without requiring heat treatment or the like. .
In the allergen inactivating agent composition of the present invention, the reason why the effect of being excellent in allergen inactivating effect is obtained is not clear, but is presumed as follows.
In a conventional allergen inactivating agent composition, a component that denatures a metal compound or allergen is blended, and the component that denatures a metal ion or allergen itself binds to a protein molecule in the allergen to act as an allergen. It is thought to be inactivated.
On the other hand, the allergen deactivator composition of the present invention comprises a heavy metal compound (A) selected from the group consisting of a water-soluble compound containing heavy metal and a water-dispersible compound containing heavy metal, and a solution dissolved in water. The pH at 25 ° C. of the diluted processing solution containing inorganic peroxide (B) that generates hydrogen oxide and diluted 10,000 times by mass with distilled water is 7.0 to 11.0.
In the allergen deactivator composition of the present invention, hydroxy radicals are generated by the interaction between hydrogen peroxide generated when component (B) is dissolved in water and heavy metal ions released from component (A). . The generation of the hydroxy radical proceeds stably in a pH range of neutral to alkaline. And since the said hydroxy radical acts on an allergen and inactivates an allergen, it is estimated that it is excellent in the effect of allergen inactivation compared with the past.

  The effect of the estimation is better obtained when the compound containing the heavy metal (transition element, typical element) exemplified in the description of the component (A) or the compound releasing the metal ion is used. Inherently obtained particularly well when using a compound containing a transition element (preferably a transition element belonging to the fourth period and group 7-11 having similar properties to each other) or a metal ion releasing compound thereof It is thought to be a thing.

In addition, the allergen inactivating agent composition of the present invention provides a higher effect of allergen inactivation when it is in the form of powder or granules. This is because, in the powdery or granular form, the inorganic peroxide (B) is dissolved in water in the composition before the allergen inactivation treatment, unlike the liquid form. This is probably because hydrogen peroxide is not generated and hydrogen peroxide can be used sufficiently and effectively when allergen inactivation treatment is performed.
Furthermore, in the allergen deactivator composition of the present invention, since the inorganic peroxide (B) that dissolves in water and generates hydrogen peroxide is used, hydrogen peroxide that is a liquid raw material is used from the beginning. Compared to the case, the decomposition of hydrogen peroxide can be suppressed to a lower level. In addition, when hydrogen peroxide as a liquid raw material is used, the form of the allergen inactivating agent composition is almost restricted to a liquid state, and it is difficult to maintain the effect of allergen inactivation or to ensure liquid stability. On the other hand, when the inorganic peroxide (B) is used, the allergen deactivator composition can be in various forms, and the maintenance or stability of the allergen deactivation effect with time is all good. .

According to the allergen inactivating agent composition of the present invention, the object to be treated is not colored, and the composition is not solidified, and an excellent allergen inactivating effect is obtained.
In addition, some of the clothes have difficulty in removing the attached pet hair, and it has been difficult to inactivate the allergen attached to the hair, but according to the present invention, such allergen can be easily removed. Can be inactivated.
The technology according to the present invention can be applied to various products exhibiting an allergen inactivating action (for example, deodorants, antibacterial / antifungal agents, cleaning liquids, insecticides, mite repellents, acaricides, dehumidifiers, air sprays) , Fragrances, quasi drugs, pharmaceuticals, etc.).

  Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples. Note that “%” means “mass%” unless otherwise specified.

<Preparation of allergen deactivator composition>
(Examples 1-7, Comparative Examples 1-4)
According to the composition shown in Table 1, each component was mixed according to a conventional method, and a powdery composition (Examples 1 to 7, Comparative Examples 1 to 3) and a liquid composition (Comparative Example 4) Were prepared respectively.
In addition, the powdery composition (powder composition) was prepared by balancing with sodium carbonate so that the total of the components described in the table was 100% by mass. Moreover, the liquid composition (liquid composition) was prepared by balancing with distilled water so that the sum total of the component as described in a table | surface might be 100 mass%.
In addition, the unit of the compounding amount in Table 1 is mass%.
The compounding amount of each component shown in Table 1 represents the compounding amount of the raw material itself (the amount of appearance) shown below.

[Description of components shown in the table].
・ Heavy metal compound (A)
Copper sulfate pentahydrate: Special grade reagent, manufactured by Kanto Chemical.
Iron (III) chloride hexahydrate: reagent grade, manufactured by Kanto Chemical.

・ Inorganic peroxide (B)
Sodium percarbonate: trade name “SPC-G”, manufactured by Mitsubishi Gas Chemical.
Sodium perborate: trade name “15% Perbon”, manufactured by Mitsubishi Gas Chemical.

・ Organic peracid precursor (C)
OBS12: Sodium dodecanoyloxybenzenesulfonate (in-house synthesized product).
OBC10: Decanoyloxybenzoic acid (in-house synthesized product).

(Method for producing organic peracid precursor)
Production example of OBS12:
Sodium 4-hydroxybenzenesulfonate (reagent, manufactured by Kanto Chemical), N, N-dimethylformamide (reagent, manufactured by Kanto Chemical), decanoic acid chloride (reagent, manufactured by Tokyo Chemical Industry), and acetone (reagent, Kanto Chemical Co., Ltd.) and the following method.
3,000 g (15.3 mol) of sodium 4-hydroxybenzenesulfonate dehydrated in advance was dispersed in 9000 g of N, N-dimethylformamide, and 2918 g (15.3 mol) of decanoic acid chloride was added at 50 ° C. while stirring with a stirrer. It was added dropwise over 30 minutes. Reaction was performed after completion | finish of dripping for 3 hours, and N, N- dimethylformamide was distilled off at 100 degreeC under pressure reduction (0.5-1 mmHg). After washing with acetone, it was purified by recrystallization in a water / acetone (= 1/1 molar ratio) solvent.

Production example of OBC10:
Synthesis was performed according to the method described in JP-A-10-231274.
That is, 4-hydroxybenzoic acid was suspended in capric acid [4-hydroxybenzoic acid / capric acid (= 1/1 molar ratio)] and heated to 110 ° C. Subsequently, capric acid chloride [4-hydroxybenzoic acid / capric acid chloride (= 1/1 molar ratio)] was added dropwise. The mixture was heated and stirred at the same temperature for 4 hours. After cooling, the deposited precipitate was collected by filtration and washed with hexane. Further, this reaction product was dried to obtain crude 4-decanoyloxybenzoic acid.
100 g of the obtained crude 4-decanoyloxybenzoic acid was placed in a 3 liter four-necked flask, 1500 g of ethanol was added, and the mixture was dissolved by heating at 70 ° C. After dissolution, 300 g of water was added and allowed to cool to precipitate crystals. The crystals were collected by filtration and dried to obtain purified 4-decanoyloxybenzoic acid.

-Other ingredients Sodium carbonate: reagent grade, manufactured by Kanto Chemical.
Distilled water

<Preparation of diluted solution>
Using the powder compositions (Examples 1 to 7, Comparative Examples 1 to 3) and the liquid composition (Comparative Example 4) obtained above, dilution treatment solutions were prepared as follows. The following dilution treatment was performed on a mass basis.
When a powder composition is used, 1000 g of the powder composition obtained above is reduced four times, and 50 g of the reduced powder composition is dissolved in distilled water and first diluted 100 times. (A) 5000 g was prepared, and 1 g of the aqueous solution (a) was further diluted 100 times with distilled water (diluted 10000 times in total) to prepare 100 g of the aqueous solution (b) to obtain a diluted treatment solution. .
When the liquid composition was used, 100 g of a diluted treatment solution was prepared by diluting 0.01 g of the liquid composition 10,000 times with distilled water.

<Measurement of pH of diluted solution>
The measurement of the pH of the dilution treatment liquid was carried out by adjusting the dilution treatment liquid to 25 ° C. and using a glass electrode type pH meter (product name: F-52, manufactured by Horiba, Ltd.) in accordance with JIS K3362-1998. The measurement was performed by immersing the glass electrode in a dilution treatment solution and reading the pH value indicated after 1 minute. The results are also shown in the table.

<Evaluation of allergen inactivation effect>
As the sample solution in the evaluation, the dilution treatment liquid obtained above (the composition of each example diluted 10,000 times with distilled water) was used.
To each sample solution, 1 ppm of mite antigen (trade name: Der f2, manufactured by Seikagaku Corporation) or 1000 ppm of house dust was added and allowed to stand at 25 ° C. for 2 hours.
In addition, what collected (sample) the house dust from the floor of the household which has raised the cat in the household using the vacuum cleaner (product name: Sanyo SC-KH4-SB) was used. For house dust, a dispersion was prepared by suspending the sample in distilled water, and an addition amount having a desired concentration was collected and added to the sample solution.
After standing, a sample solution to which 1 ppm of mite antigen was added or a sample solution to which 1000 ppm of house dust was added was added with a 10000 ppm aqueous solution of sodium thiosulfate in the same amount (0.5 g) as these sample solutions. Diluted 50-fold by volume with 0.2 M phosphate buffer (pH 7.4) containing 10% bovine serum albumin (BSA) (this 50-fold diluted solution is referred to as “ELISA sample”) for antigen-antibody reaction. It used for the measurement (ELISA).
In the present specification, “ppm” refers to the content ratio of mite antigen or house dust per liter of sample solution and the content ratio of sodium thiosulfate per liter of aqueous solution (both based on mass: mg). The ratio of the component contained in (mass basis: mg).

(Measurement of antigen-antibody reaction)
The measurement of ELISA was performed using a general sandwich method (Japanese translations of PCT publication No. 2004-510717 gazette). The outline is as follows (1) to (11).
When using a sample solution to which 1 ppm of mite antigen is added:
(1) A 96-well (Nunk, Maxisorp 441404) was diluted 400 times with 0.05M carbonate buffer (1.59 g sodium carbonate and 2.93 g sodium bicarbonate dissolved in 1 L distilled water). 100 μL of Der f2 antibody (manufactured by Seikagaku Corporation, 15E11) solution was added and left at 4 ° C. for 16 hours. Thereafter, the solution in each well was discarded.
(2) Next, each well was washed by adding 350 μL of 0.2 M phosphate buffer (pH 7.4) containing 500 ppm of Tween 20 (trade name, manufactured by Merck). Thereafter, the solution in each well was discarded. The same operation was repeated twice.
(3) Next, 150 μL of a phosphate buffer solution (pH 7.4) containing 1% by mass of BSA was added to each well and left at room temperature for 1 hour. Thereafter, the solution in each well was discarded.
(4) Next, each well was washed by adding 350 μL of 0.2 M phosphate buffer (pH 7.4) containing 500 ppm of Tween 20 (trade name, manufactured by Merck). Thereafter, the solution in each well was discarded. The same operation was repeated twice.
(5) Next, 100 μL of the above-mentioned ELISA sample was added to each well and allowed to stand at room temperature for 1 hour. Thereafter, the solution in each well was discarded.
(6) Next, 350 μL of 0.2 M phosphate buffer (pH 7.4) containing 500 ppm of Tween 20 (trade name, manufactured by Merck) was added to each well and washed. Thereafter, the solution in each well was discarded. The same operation was repeated twice.
(7) Next, a Der f2-labeled antibody solution (Seikagaku Corporation, 13A4PO) diluted 200-fold with a phosphate buffer solution (pH 7.4) containing 1% by mass of BSA was added to each well. Left for hours. Thereafter, the solution in each well was discarded.
(8) Next, each well was washed by adding 350 μL of 0.2 M phosphate buffer (pH 7.4) containing 500 ppm of Tween 20 (trade name, manufactured by Merck). Thereafter, the solution in each well was discarded. The same operation was repeated twice.
(9) Subsequently, o-phenylene was added to 12 mL of a substrate buffer solution (10.3 g of citric acid / monohydrate and 35.8 g of disodium monohydrogen phosphate / 12 dodecahydrate dissolved in 1 L of distilled water). A solution was prepared by dissolving 1 tablet of diamine (manufactured by Cosmo Bio, color developing component) and further adding 12 μL of 30% by mass hydrogen peroxide.
(10) To each well, 100 μL of the solution prepared in (9) was added and left for 15 minutes at room temperature, protected from light.
(11) Thereafter, 150 μL of 1 M sulfuric acid was added to each well, and the absorbance at 492 nm was measured using an absorbance meter (product name: Power Scan HT).

When using a sample solution containing 1000 ppm house dust:
In the above measurement method when a sample solution to which 1 ppm of mite antigen is added is used, instead of o-phenylenediamine as a coloring component in (9), ABTS (2,2′-azino-bis (3-ethylbenzthiazole-6) The absorbance at 409 nm was measured by the same measurement method except that -sulphonic acid)) was used.
In addition, about the cat allergen (Fel d1) in house dust, it quantifies beforehand using the kit (brand name: EL-FD1) by an indoor company, Based on the result of this quantification, allergen inactivation The effect was evaluated.

The inactivation rate (%) of each allergen was determined from the following formula. The results are also shown in the table.
Inactivation rate of allergen (%) = (OD value of control−OD value of sample for ELISA) / (OD value of control) × 100
Here, the “OD value of the ELISA sample” refers to a value obtained by subtracting the blank OD value from the absorbance value measured in the antigen-antibody reaction measurement (11).
The “control OD value” means that the blank OD value is subtracted from the absorbance value measured in (11) when distilled water is used instead of the ELISA sample in the antigen-antibody reaction measurement (5). Value.
The “blank OD value” refers to the absorbance value of an empty well not subjected to a reaction.
If the inactivation rate (%) of the allergen obtained from the above formula was 50% or more, it was determined that the allergen inactivation effect was good.
In the table, “-” indicates that evaluation is not performed.

  As is clear from the above results, the allergen inactivating agent composition and the allergen inactivating method of the present invention have a high effect of inactivating mite allergen (Der f2) and cat allergen (Fel d1). I was able to confirm.

In particular, Examples 1, 2, 6, and 7 in which the (A) component and the (B) component in the present invention were used in combination from Examples 1, 2, 6, and 7 and Comparative Examples 1 and 4 It turns out that the effect of allergen inactivation is remarkably high compared with the comparative examples 1 and 4 which lack either (A) component or (B) component.
Further, from the comparison between Example 2 and Example 3 and the comparison between Example 1 and Example 4, when the organic peracid precursor (C) is further contained, the effect of allergen inactivation becomes higher. Was confirmed.

  Therefore, according to the present invention, it is possible to provide an allergen inactivating agent composition excellent in the effect of allergen inactivation and a simple allergen inactivating method without requiring heat treatment or the like.

Claims (5)

A heavy metal compound (A) selected from the group consisting of water-soluble compounds containing heavy metals and water-dispersible compounds containing heavy metals;
An inorganic peroxide (B) that dissolves in water and generates hydrogen peroxide,
And the pH at 25 degreeC of the dilution process liquid diluted 10000 times on the mass basis with distilled water is 7.0-11.0, The allergen inactivating agent composition characterized by the above-mentioned.   The allergen inactivating agent composition according to claim 1, further comprising an organic peracid precursor (C).   The allergen inactivation according to claim 1 or 2, wherein the heavy metal compound (A) is at least one water-soluble salt selected from the group consisting of a water-soluble salt containing copper and a water-soluble salt containing iron. Agent composition.   The allergen inactivating agent composition according to any one of claims 1 to 3, which inactivates an allergen released from an organism or an allergen derived from a dead body of an organism.   The allergen inactivation method which uses the allergen inactivation agent composition in any one of Claims 1-4.