JP2009195151A - Apparatus for collecting tissue from rodent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To simply collect a tissue from a tail or ears of a rodent such as a mouse without mixing other individual tissues therein. <P>SOLUTION: An apparatus for collecting the tissue from the rodent is obtained by supporting a pair of cutting blades 11 and 11 on a support 8 in a separated state so that a tissue collection site of the rodent can be inserted. The apparatus is designed to make relative movement of the cutting blades 11 and 11 in the state thereof with an operator 6, cut the inserted tissue collection site, and house the cut tissue collection site in a housing body 9 attached to the support 8. The cutting blades 11 and 11, and the support 8 are removed from the operator 6, and a new support 8 in which new cutting bodies and a new housing body 9 are attached is mounted on the operator 6 to collect the tissue from the next individual. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention belongs to the technical field of a device for collecting tissue from rodent (rodent) animals such as mice and rats.

Today, along with the development of genome research, small rodents such as mice and rats that have been genetically manipulated to study gene functions have been developed. As these rodent animals, for example, if it is a mouse, a transgenic mouse that introduces a gene into a fertilized egg, a knockout mouse that introduces a gene into an embryonic stem cell, etc., and eliminates the expression of that gene by homologous recombination, and further There are knock-in mice incorporating these genes, and these mice are used to analyze the functions of genes targeted for manipulation at the individual level. In order to use these genetically engineered mice for research, the stability of the engineered genes is required, and it is necessary to create many strains of mice from the genetically engineered mice created first. This creation operation is a so-called breeding operation, and if it is a mouse having fertility, it is possible to create offspring according to the plan. These progeny should be inherited from genetically engineered parents according to Mendel's law, and the method of selecting individuals having these engineered genes greatly affects the reproduction efficiency and the production efficiency of various strains of mice.
This selection work is performed using a part of the tissue collected from a living mouse, and the manipulation gene in the genomic DNA can be made possible only by a highly sensitive gene amplification operation called polymerase chain reaction (PCR). Are known. This PCR-based gene amplification test method is a so-called parent-child test and is highly sensitive. Therefore, it is absolutely necessary to avoid contamination of tissues from other individuals. If even a small amount of tissue from another individual is mixed, an individual who has not inherited the manipulated gene may be misdiagnosed (incorrectly determined) as a positive individual due to its sensitivity.
If genetically manipulated mice misdiagnosed in this way are mixed, the subsequent breeding plan will become more complicated, and the current situation is to extract DNA using tissues collected from the tail and ear lobe of the mouse. The target gene is detected. In this case, for example, tissue is collected from a cut tail or earlobe of a mouse. Conventionally, a cutting tool such as a razor or scissors has been used to cut the tail or earlobe.

However, when cutting the tail or earlobe in this way, it is important to prevent contamination by other individual tissues, for example, when collecting tissue from the cut tail of a mouse. For razors and scissors, etc. to be used for cutting, it is necessary to use different tools for each individual, and many tools are required. However, when using a razor, it is difficult to cut the bone at the tail. In addition, advanced techniques are also required for excision of the earlobe. Attempts to use scissors have been made. However, discarding a single use such as scissors is costly and will be reused. In this case, the scissors used for cutting are used. Since the blood and tissue of the individual adheres, there is a problem that workability is poor because it needs to be reused after a rigorous cleaning process.
In actual situations, it is often required to cut the tails of several tens or hundreds of mice at once to collect tissues, and in such cases, workers are particularly hard-worked. There is also a problem that the present invention is not solved.

The present invention was created with the object of solving these problems in view of the above circumstances, and the invention of claim 1 provides a pair of cut bodies as a tissue collection site for rodents. A support that is spaced apart so that it can be inserted, an operating body that performs a relative movement operation of the cut body so as to cut the inserted tissue collection site, and a support that is attached to the support for receiving the cut tissue collection site. A tissue collection device from rodents, characterized in that the cutting blade is detachably attached to the operating body.
In a second aspect of the present invention, the pair of cutting bodies are cutting blades arranged to face each other, and the operation body is a pair of operation parts pivotally supported at the intermediate part so as to be swingable. Each has a finger insertion portion formed on the proximal end side, and a locking portion for engaging the pair of cutting blades on the distal end side, and the cutting blade is placed on the opposite side based on the proximity operation of the finger insertion portion. The apparatus for collecting tissue from rodents according to claim 1, wherein the apparatus is cut by moving to a rod.
The rodent animal according to claim 1 or 2, wherein the cutting blade is provided with a burn means, and blood is stopped by burning the cutting site remaining in the animal. From the tissue collection device.
According to a fourth aspect of the present invention, the support body includes an upper support body that supports the upper blade body in a state in which the upper blade body is prevented from falling off, and a lower support body that removably supports the lower blade body, and these upper and lower side support bodies. The body is such that the upper blade body and the lower blade body face each other with the tissue collection site to be cut up and down, and the operating body pushes the upper blade body toward the lower blade body to collect the tissue. The tissue from a rodent animal according to claim 1, wherein the upper blade body is left on the lower blade body side by releasing the pushing movement of the operating body. It is a collection device.
According to a fifth aspect of the present invention, the tissue collection site is an ear, and the support is provided with tag attachment means for attaching the tag with the ear site remaining on the animal side sandwiched up and down. A tissue collection device from the described rodent.

According to the invention of claim 1, the target tissue can be easily cut by operating the operating body, and after cutting, the support body and the operating body are cut only by exchanging the cutting body and the containing body. There is no adhesion of the blood or tissue of the individual, and it can be reused as it is, which is economically advantageous.
By setting it as invention of Claim 2, the structure | tissue can be cut | disconnected by scissors sense and it becomes the thing excellent in operativity.
By setting it as invention of Claim 3, the blood-stopping of the cutting | disconnection site | part which remains in an animal can be performed, and it can contribute to animal protection.
By setting it as invention of Claim 4, the structure | tissue cutting | disconnection can be performed with a push operation feeling, and it becomes the thing excellent in operativity.
By setting it as invention of Claim 5, tag attachment can be performed simultaneously with tissue cutting | disconnection, and identification of the animal and tissue cut | disconnected tissue which cut | disconnected tissue is ensured.

  Next, embodiments of the present invention will be described with reference to the drawings. In the first embodiment shown in FIGS. 1 to 4, 1 is a tissue collection device from the tail of a rodent, and the collection device 1 of the present embodiment includes an operation tool 2, a cutting device The operation tool 2 employs a scissors type formed of an insulating material in the present embodiment, and intersects with the pivot 5 as a rocking fulcrum. It is comprised using a pair of operation body 6 pivotally supported by the shape. A finger insertion portion 6 a for inserting a finger is formed in a ring shape at the base end portion of each operation body 6. In addition, a locking portion is formed at the distal end of each operation tool 6 so as to protrude in the same vertical direction (upward in the present embodiment). The locking portion is a protruding proximal end in the present embodiment. Is a small-diameter engaging portion 6b, and its tip is a large-diameter engaging portion 6c. A plate-shaped ammunition machine 7 is interposed between the base end portions of the pair of operation bodies 6, but one end 7a of the ammunition machine 7 is fixed to one operation body 6 (such as welding or screw tightening). Appropriate fixing means can be adopted). On the other hand, the other end portion 7b of the ammunition machine 7 is configured to face the inner surface of the other operating body 6 and be detachable, but the present invention even if it is fixed like the one end portion 7a. Can be implemented. In the natural state in which the operation unit 6 has the other end 7b of the ammunition just in contact with the inner surface of the operation body 6 and no load is applied to the operation unit 7, the finger insertion portions 6a are separated from each other. When the finger insertion portion 6a is operated close to each other, the operating body 6 swings and deforms in the direction of narrowing the facing interval while being pressed by the bullet machine 7, but conversely, when the finger insertion portion 6a is operated to be separated. In the state in which the other end portion 7b of the ammunition is separated from the other operating body 6, the shape of the swing is changed in a direction in which the facing interval is widened.

  The cutting part 3 includes a support body (holder) 8 formed of an insulating material. The support body 8 has a cylindrical shape, and a lower end thereof accommodates a cut tail. A possible container 9 is detachably attached. In the present embodiment, the container 9 is provided with a label 9a (for example, a bar code or something that can be written as needed) for identifying the collected individual. Of course, it is also possible to print it in advance. Incidentally, as the container 9, for example, a test tube or a bottomed tube (bag) is represented, and when the tube is flexible, a tube with a chuck 9b that can be opened and closed at the entrance (opening) is adopted. In this way, after the container 9 is removed from the support 8, the entrance of the container 9 can be closed, which is convenient.

On the other hand, the support 8 is formed with a cylindrical portion 8a, and a support groove 8b is formed in a stepped shape on the upper end surface portion, but the support groove 8b is smaller than the inner dimension of the support cylindrical portion 8a. The auxiliary plate 10 formed with a through hole 10a having a size that can penetrate the cut portion of the mouse tail is detachably attached in a state where it is lightly press-fitted.
Further, a pair of left and right guide grooves (which may be guide holes) 8c extending from the outer peripheral surface to the inner peripheral surface of the cylindrical portion 8a are formed at the upper end portion of the support body 8. A cutting blade 11 is incorporated in 8c so as to be movable in the left-right direction. Incidentally, the guide groove 8c is set to have a groove width wider than the diameter of the through hole 10a, and a groove groove 8d having a wider groove depth intermediate portion is formed, and the cutting blade 11 is lightly supported in the groove 8d. When the cutting blade 11 is inserted and incorporated in a shape, the cutting blade 11 can be moved in the left-right direction in a state where inadvertent movement is restricted, and when the cutting blade 11 is inserted and removed, the cutting blade 11 is inserted into the through hole 10a of the cutting blade 11. It is considered that the corresponding part (part corresponding to the tail cutting of the cutting blade 11) does not contact the groove bottom surface and the side surface of the guide groove 8c, that is, the tail cutting part of the cutting blade 11 does not contact the support 8.
Further, a positioning mark 11a is provided on the surface (upper surface) of the cutting blade 11, and the mark 11a is aligned with the upper and outer end positions 8e of the guide groove 8c as in the present embodiment, for example. The cutting blade 11 is accurately positioned at the pre-cutting position with respect to the support 8.

Moreover, although the locking hole is formed in the outer site | part which protrudes outward from the outer side end of the support body 8 of the cutting blade 11, the locking hole of this Embodiment is the outer wide hole part 11b. The inner narrow hole portion 11c is formed to be continuous in the left-right direction. As described above, with respect to the operating body 6, the finger insertion portion 6a is expanded to align the large diameter locking portion 6c with the wide hole portion 11b, and the large diameter locking portion 6c passes through the wide hole portion 11b. Thus, when the small diameter locking portion 6b is positioned at the position of the wide hole portion 11b, and the finger insertion portion 6 is operated close to the position where the other end portion 7b of the ammunition just contacts the other operation body 6, The locking portion 6b is moved to the inner end position of the narrow hole portion 11c, so that the cutting blade 11 is set so as to be prevented from coming off from the operating body 6. In this locked state, after inserting the tail X of the mouse into the auxiliary plate through hole 10a, when the finger insertion portion 6a is operated in proximity, the cutting blades 11 move inward (relative movement) to each other, and the blade portion is moved. The tail X can be cut by crossing (or hitting) each other.
Incidentally, in this case, the cutting position is set so as to be just positioned by operating until the tip of the operating body 6 comes into contact with the outer surface of the support 8.

  Furthermore, in the present embodiment, a hemostasis means 12 is provided that can hemostaze the cut portion of the tail X. The hemostasis means 12 used here is adapted to stop hemostasis by burning a cut surface remaining on the animal side of the tail X by instantaneously supplying a high-frequency current in synchronism with the cutting, and generating a high-frequency current. It comprises a device 13, a lead wire 14 connected to the high-frequency current generator 13, and an engaging portion (for example, an alligator jack) 15 provided at the tip of the lead wire 14. 11 is engaged with the outer end, and a high-frequency current flows by contact between the cutting blades 11 accompanied by the cutting operation, whereby hemostasis can be performed. The hemostatic means is not limited to that burned by a high-frequency current, and any means may be used as long as it can be burned using high-temperature generating means such as an electric heater.

  In the embodiment of the present invention configured as described above, the container 9, the auxiliary plate 10, and the cutting blade 11 are set on the support 8, and the operating body 6 is locked to the set cutting blade 11. Further, the engaging portion 15 is engaged. In this state, after inserting the rat's tail X into the auxiliary plate through hole 10a, when the finger is inserted into the finger insertion portion 6a and the operation body 6 is closed, the cutting blade 11 intersects and the tail X is cut. The cut tail X portion falls into the container 9. At this time, high frequency current flows through the cut portion of the tail X remaining on the rat side, and hemostasis due to burns is performed.

  In the case where the present invention is implemented as described above, the rat tail X can be easily cut. However, after this cutting operation, the operation body 6 may be removed from the cutting blade 11, and thereby a pair of protrusions may be removed. The combined cutting blade 11 closes the insertion portion of the tail X, functions as a lid, and can be stored as it is.

  Further, if necessary, the container 9 can be detached from the support 8 and the entrance can be closed with the chuck 9a. Further, the cutting blade 11 is removed from the support body 8 while removing the operating body 6. When the cutting blade 11 is extracted from the support body 8, the guide groove 8 c is a recess provided at an intermediate position in the vertical direction of the guide groove 8 c. Since the cutting blade 11 is inserted and supported by the groove 8d and wider than the auxiliary plate through hole 10a into which the tail X is inserted, the tail of the cutting blade 11 is formed on the groove bottom surface and the groove side surface of the guide groove 8c. As a result, not only the operating body 6 but also the support body 8 in which the auxiliary plate 10 has been replaced can be used again to cut the tail X of another mouse.

  5 to 11 show a second embodiment of the present invention. This is a tissue collection device by cutting the earlobe Y of a rodent, and the collection device 20 of the present embodiment is composed of an operation tool 21 and a cutting part 22. The tool 21 includes an upper support portion 23 and a lower support portion 24. Support rods 23a and 24a project from these support portions 23 and 24, and the support rods 23a and 24a are respectively connected to rotating bodies 23b and 24b that are provided so as to be relatively rotatable in the vertical direction about the support shaft 25. ing. A return ammunition (not shown) is interposed between the rotating bodies 23b and 24b, and urges the upper and lower support portions 23 and 24 to be positioned on the side away from each other.

The upper support portion 23 is provided with a through hole 23c penetrating in the vertical direction, and the operating rod 25 penetrates the through hole 23c so as to be movable in the vertical direction. An operating body 25 a is integrally provided at the upper end penetrating, and a bullet machine 25 b is interposed between the operating body 25 a and the upper surface of the upper support portion 23.
On the other hand, a groove 23d having an opening on the lower side and having a top surface is formed at the lower end of the upper support 23, and the lower surface of the upper support 23 is made of a rubber elastic material. A support plate 26 having a support hole 26a for fitting and supporting the supported portion 27a of the blade body 27 is detachably fixed.

  Reference numeral 27 denotes an upper blade body. The upper blade body 27 has a cylindrical shape, and a supported portion 27a having a small diameter is formed on the upper surface so as to protrude. The upper blade body 27 is supported by the upper support portion 23 by forcibly fitting the supported portion 27a into the support hole 26a. Furthermore, 28 is a tag, and the tag 28 can describe necessary information such as work date and time, a specific marker of the mouse, etc. (may be printed), and the tag 28 is supported. A through hole 28a through which the portion 27a passes is formed. The tag 28 is set on the upper blade body 27 in a state where the supported portion 27a passes through the through hole 28a, and the upper blade body 27 is supported on the upper support body 26 by supporting the upper blade body 27 on the upper support body 26 as described above. The blade 27 is supported in a sandwiched manner. In this supported state, the tag 28 is set to a size that protrudes outward from the groove 23d.

  On the other hand, the lower support 24 has a cylindrical shape, and has a first support groove 24a for positioning and supporting the lower blade 29 on its upper surface, and a smaller diameter than the first support groove 24a. Thus, a second support groove 24b is formed for positioning and supporting the flange 31 fitted and fixed to the opening of the storage body 30.

  The lower blade body 29 is formed with a cutting hole 29a for the upper blade body 27 to fit and cut (shear) the earlobe Y of the mouse before cutting set on the upper surface of the lower blade body 29. However, in this case, a positioning groove 29b is formed on the upper surface of the lower blade 29. The positioning groove 29b penetrates the earlobe Y1 remaining on the mouse after cutting and the tag 28 in a retaining manner. A retaining portion 32a (represented by an arrowhead shape) is provided at a predetermined interval. After the earlobe Y2 is cut off, when the upper support portion 23 and the lower support portion 24 are brought closer together, the retaining portion 32a penetrates the remaining earlobe Y1 and the tag 28 and enters the groove portion 23d. The stopper 32 for preventing the 28 earlobe Y1 from coming off can be positioned and set. In the present embodiment, the stopper 32 has a ring shape, but it is of course possible to have a gap.

  In the second embodiment of the present invention configured as described above, the upper blade 27 is set on the upper support 23 together with the tag 28, while the housing 30 is fixed to the lower blade 28. After setting the collar 31 and the lower blade body 29 and further setting the stopper 32 on the lower blade body 29, the earlobe Y is set between the upper and lower blade bodies 27 and 29 (see FIG. 6). At this time, the earlobe Y is set so as to protrude from the retaining portion 32a. In this state, when the upper support 23 is relatively pushed down toward the lower support 24 and the upper blade 27 is inserted into the cutting hole 29a, a part of the earlobe Y2 is cut and dropped into the container 30 (FIG. 7). reference). When the upper support 23 is further pushed down from this state, the earlobe Y1 and the tag 28 where the retaining portions 32a remain are penetrated in a retaining shape (see FIG. 8). Thereafter, when the operating body 25a is pushed against the ammunition 25b, the upper blade supported part 27a is pushed and comes out of the supporting part 26 (see FIG. 9). The lower blade 29 moved downward at this time is lightly supported by the cutting hole 29a and covers the cutting hole 29a. Thereafter, the push operation of the operating body 25a and the upper support body 23 is released to return to the base position (see FIG. 10), and then the earlobe Y1 with the tag 28 is removed from the lower blade body 29 and the lower support body If the collar part 31 and the lower blade body 29 are removed as a set from 24, the storage body 30 containing the cut earlobe Y2 covered by the upper blade body 27 can be taken out.

  As described above, in the second embodiment in which the present invention is implemented, the earlobe can be cut with one touch, so that workability is improved, and the upper and lower blade bodies 27 and 29 are removed and the storage body is removed. Since it moves to the 30 side, the upper and lower supports 23 and 24 are not contaminated by the mouse and can be used repeatedly as they are.

  In addition, with this device, the tag 28 can be attached to the earlobe Y1 remaining in the mouse together with the cutting of the earlobe Y2, so that the individual identification with the earlobe Y2 that has been cut off can be easily made. Can be avoided.

It is a top view of the tissue collection device from the rodent animal of the first embodiment. It is a top view of the principal part of the tissue collection device. FIG. 3 is a cross-sectional view taken along the line AA of FIG. 2 in the tissue collection device. FIG. 3 is a cross-sectional view taken along the line BB of FIG. 2 in the tissue collection device. It is a top view of the tissue collection device from the rodent animal of the second embodiment. It is a 1st cutting process figure. It is a 2nd cutting process figure. It is a 3rd cutting process figure. It is a 4th cutting process figure. It is a 5th cutting process figure. It is a 6th cutting process figure.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Tissue extractor 3 Cutting part 8 Support body 9 Container 11 Cutting blade 23 Upper support part 24 Lower support part 27 Upper blade body 28 Tag 29 Lower blade body

Claims (5)

  A support that supports a pair of cut bodies in a spaced manner so that a tissue collection site of a rodent can be inserted; an operation body that performs a relative movement operation of the cut body so as to cut the inserted tissue collection site; An apparatus for collecting tissue from rodents, comprising: a receiving body attached to a support for receiving the cut tissue collection site, wherein the cutting blade is detachably attached to the operating body.   The pair of cutting bodies are cutting blades arranged to face each other, and the operation body is a pair of operation parts pivotally supported at the intermediate part so that the operation part has a finger on the base end side. An insertion portion is formed, and a locking portion that is locked to the pair of cutting blades is formed on the distal end side, and the cutting blade is moved to the opposite side and cut based on the proximity operation of the finger insertion portion The tissue collection device from a rodent animal according to claim 1, wherein the device is a tissue collection device.   The tissue collecting device from a rodent animal according to claim 1 or 2, wherein the cutting blade is provided with hemostatic means so as to stop blood from a cutting site remaining in the animal.   The support body includes an upper support body that supports the upper blade body in a state in which the upper blade body is prevented from falling off, and a lower support body that detachably supports the lower blade body, and these upper and lower side support bodies are tissues to be cut. The upper blade body and the lower blade body face each other with the collection site sandwiched vertically, and the operating body pushes the upper blade body toward the lower blade body to cut the tissue collection site. The apparatus for collecting tissue from rodents according to claim 1, wherein the upper blade remains on the lower blade by releasing the pushing movement of the operating body.   The rodent animal according to claim 4, wherein the tissue collection site is an ear, and the support is provided with tag attachment means for attaching the tag with the ear site remaining on the animal side sandwiched up and down. Tissue collection device.

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