JP2009162720A - Automatic analysis apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an automatic analysis apparatus that yields high-precision measurement reproducibility without increasing costs. <P>SOLUTION: The automatic analysis apparatus measures the amount of light transmitted through the reaction container 105 by disposing a reaction container 105 and a reaction container detection mechanism on the outer circumference of the reaction disk 104: disposing a detector 204 which detects passage of the reaction container 105 on a path; and a light source 113 and a spectroscopic detector 114 at positions to sandwich the reaction container 105. When rotating the reaction disk, the automatic analysis apparatus calculates the speed of rotation of the reaction disk based on the detection of the passage of the reaction container 105 by the detector 204 and corrects the timings of start and end of photometry with use of the calculated speed. When rotating the reaction disk, the automatic analysis apparatus calculates the speed of rotation of the reaction disk based on an output signal from the spectroscopic detector 114 and corrects the timings of start and end of photometry by use of the calculated speed. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an automatic analyzer, and in particular, a plurality of reaction vessels are arranged on the circumference of the outer periphery of a reaction disk, and a light source and a spectroscopic detector are arranged across the reaction vessel to measure the amount of light transmitted through the reaction vessel. The present invention relates to a measurement method of a rotating reactor.

  Conventionally, a rotary reactor is known in which a plurality of reaction vessels are arranged on the circumference of the outer periphery of a reaction disk, and a light source and a spectroscopic detector are arranged with the reaction vessel interposed therebetween to measure the amount of light transmitted through the reaction vessel. . In measurement using such a reactor, the cell position is detected from the detection plate corresponding to each cell, and the photometric start position is determined. There is also a measurement method in which a cell position is detected from an encoder signal and photometric data to determine a photometric start position.

  However, there are errors in the size of the detection plate, the size between the detection plate and the cell, and the cell attachment position. For this reason, when each error is taken into consideration, it is necessary to shorten the time from the start to the end of photometry. In the current method, in order to cancel out these errors as much as possible, the time from the detection plate position to the photometric start position can be adjusted. However, when the total number of reaction vessels increases, the dimensional errors in all the reaction vessels cannot be offset. In order to solve this problem, even when a system that individually adjusts the time for each cell is installed, variations due to uneven speed of the reaction disk remain, and the photometric time must be shortened accordingly. . In addition, there is a method of directly detecting the position information from the encoder and calculating the photometric start position, but an encoder having the necessary resolution is very expensive and not practical in terms of cost.

  An object of the present invention is to provide an automatic analyzer that can achieve high-precision measurement reproducibility without increasing costs.

  In the present invention, the reaction vessel and the reaction vessel detection mechanism are arranged on the circumference of the outer periphery of the reaction disk, and at least one detector for detecting the reaction vessel position is arranged on the track, and the light source and the spectral detection are sandwiched between the reaction vessels. In the measurement method that measures the amount of light in the reaction vessel by arranging the vessel, the reaction disc rotation speed is sequentially calculated from the reaction vessel position interval time obtained from the detector during reaction disc rotation, and the photometry start position from the calculated speed And determining the photometric end time.

  That is, in the present invention, a reaction vessel and a reaction vessel detection mechanism are arranged on the circumference of the outer periphery of the reaction disk, at least one detector for detecting the passage of the reaction vessel is arranged on the track, and the reaction vessel is sandwiched between them. In an automatic analyzer that measures the amount of light transmitted through the reaction vessel by arranging a light source and a spectroscopic detector at a position, the reaction disc rotation speed is based on detection of passage of the reaction vessel by the detector when the reaction disc rotates. Is an automatic analyzer that corrects the timing of the start and end of photometry using the calculated speed.

  A reaction vessel and a reaction vessel detection mechanism are arranged on the circumference of the outer periphery of the reaction disk, and at least one detector for detecting the passage of the reaction vessel is arranged on the orbit, and a light source and spectral detection are arranged at a position sandwiching the reaction vessel. In the automatic analyzer that measures the amount of light that passes through the reaction vessel by arranging a vessel, when the reaction disk rotates, the reaction disk rotation speed is calculated based on the output signal of the spectroscopic detector, and the calculated speed is used. This is an automatic analyzer that corrects the timing of the start and end of photometry.

  A reaction vessel and a reaction vessel detection mechanism are arranged on the circumference of the outer periphery of the reaction disk, and at least one detector for detecting the passage of the reaction vessel is arranged on the orbit, and a light source and spectral detection are arranged at a position sandwiching the reaction vessel. In the automatic analyzer for measuring the amount of light transmitted through the reaction vessel by arranging a vessel, the output signal of the spectroscopic detector is detected between the reaction vessel and the adjacent reaction vessel when the reaction disk rotates. The automatic analysis device calculates the reaction disk rotation speed based on the detection result and corrects the timing of the photometry start and the photometry end using the calculated speed.

  According to the present invention, it is possible to correct the variation in the photometry start time and the photometry end time due to the speed unevenness, so that the photometry possible time can be lengthened and the data reproducibility can be improved. In addition, since this system can be realized with the current mechanism without adding parts such as an encoder and a smoothing control motor controller, the cost does not increase.

The best mode for carrying out the present invention will be described.
FIG. 1 is a schematic diagram of the overall configuration of the automatic analyzer. First, I will explain the automatic analyzer and outline the flow of photometry. In FIG. 1, when an analysis measurement item of a sample is selected from the operation unit 101 and is executed by pressing a start button, the sample is transmitted from the interface 102 to the analysis unit 103. In the analyzer, the reaction disk 104 is rotated in accordance with the analysis instruction, and the sample dispensing 106, the reagent dispensing 109, and the stirring 112 are sequentially performed in the reaction container 105. Then, using the light source 113 and the multi-wavelength photometer 114, the absorbance of the reaction vessel 105 passing through is measured, and the concentration calculation is performed. The reaction container 105 after the measurement is cleaned by the cleaning mechanism 115. The analysis unit 103 transmits the concentration calculation result of the analysis item of the sample to the operation unit 101 from the interface 102, so that the user can know the concentration of the requested analysis item of the sample. Reference numeral 107 denotes a specimen container support disk, 108 denotes a specimen container set position, 110 denotes a reagent container support disk, and 111 denotes a reagent container line and position.

  FIG. 2 is a schematic diagram of a reaction disk, a detector, and a reaction vessel (cell). FIG. 3 is an explanatory diagram showing the absorbance when passing through the reaction container and the clock at the detection timing of the detection plate. FIG. 4 is an explanatory diagram of a case where a shift due to speed unevenness occurs in the metering timing to the optimum meterable region in FIG.

  In the current automatic analyzer, the detection plate 202 and the reaction vessel 203 correspond to the reaction disk 201 in a one-to-one relationship, and the same number is set. When the detection plate 202 is detected by the detector 204 while the reaction disk 201 is rotating, a detection signal of the detector is output as shown in FIG. The time at which the set timing (T1) is digested from the output fall is set as the photometry start timing. This T1 may exist only for one value for all reaction vessels, or may exist after being adjusted for each cell. Basically, this adjustment value is not changed unless parts are replaced. On the other hand, when the motor is driven at a constant speed, there is a considerable amount of speed unevenness. The driving of the reaction disk is no exception, and as a result, the photometry start timing varies as shown in FIG. The detector pulse width depends on the peripheral speed and decreases when it is fast. Since the photometry time is normally fixed, the timing of the photometry start and the photometry end also varies. In consideration of this variation, in order not to measure the light other than the flat part in the cell, the photometric time must be reduced, and the integrated average effect of the photometric data may be reduced accordingly, which may be disadvantageous in terms of reproducibility. There is.

  Here, if the speed unevenness is calculated and at least one of the photometry start timing and the photometry end timing is corrected based on the result, it is not necessary to consider the variation, so that the photometry possible time is increased and the reproducibility can be further improved.

Next, this embodiment will be described.
Usually, when the work for adjusting the photometric start timing is performed, the reaction disk is rotated at the same speed as at the time of analysis. In the case of a system in which the photometry start timing can be determined for each reaction vessel, the specific time width Tx (N) from the detection plate detection output falling signal is stored for the number N of reaction vessels. At this time, the detection time width of each detection plate is stored as the reference time width T _D1 (N) by the number of reaction vessels. During the analysis operation, the detection time width of each detection plate slightly deviates from the reference time width T _D1 (N) due to speed unevenness. The detection time width T _D2 (N) of each detection plate at that time is calculated and compared with the reference time width T _D1 (N) stored at the time of adjustment. For example, when the analysis operation is rotating faster, the photometric start timing is delayed if Tx (N) is fixed. In order to prevent this, the photometry start timing can be prevented from shifting by correcting Tx (N) by (T _D1 (N) −T _D2 (N)). As this correction means, for example, as shown in FIG. 5, a method of giving a specific time width Tx (N) by a clock pulse and increasing or decreasing the number of pulses according to the speed unevenness can be considered.

  This method can be applied as follows. As shown in FIG. 4, if the rotation speed is increased due to speed unevenness, even if the metering start timing is corrected by the method of the present embodiment, the metering end timing is set to the meterable region when the metering time is fixed. Since it is outside, it causes data fraud. Therefore, if the photometry time is also defined by the clock pulse and is controlled to increase or decrease by speed unevenness correction, it is possible to prevent data fraud due to speed unevenness more than expected.

  In this embodiment, the correction value is determined using the detection plate, but the speed can also be calculated from the photometric data of the reaction vessel. In the photometric data of the reaction vessel itself, the waveform changes due to the concentration of the reaction solution or the deterioration of the reaction vessel and is difficult to use as reference data. Therefore, a signal is used to detect between the reaction vessel and the adjacent reaction vessel. Speed unevenness correction may be performed. Therefore, it is considered that the method of using the photometric data for the correction value without using the detection plate has the same effect.

The schematic diagram of an automatic analyzer. Schematic diagram of reaction disk, detector, and reaction vessel. Explanatory drawing when peripheral speed is normal. Explanatory drawing when peripheral speed is quick. Explanatory drawing of the correction image when peripheral speed is quick.

Explanation of symbols

101 operation unit, 102 interface, 103 analysis unit, 104 reaction disk, 105 reaction vessel, 106 sample dispensing, 109 reagent dispensing, 112 stirring, 113 light source, 114 multi-wavelength photometer, 115 cleaning mechanism.

Claims (3)

A reaction vessel and a reaction vessel detection mechanism are arranged on the circumference of the outer periphery of the reaction disk, and at least one detector for detecting the passage of the reaction vessel is arranged on the orbit, and a light source and spectral detection are arranged at a position sandwiching the reaction vessel. In an automatic analyzer for measuring the amount of light transmitted through the reaction vessel by arranging a vessel,
An automatic analysis characterized in that, when the reaction disk rotates, the reaction disk rotation speed is calculated on the basis of detection of the passage of the reaction container by the detector, and the timing of photometric start and photometry end is corrected using the calculated speed. apparatus. A reaction vessel and a reaction vessel detection mechanism are arranged on the circumference of the outer periphery of the reaction disk, and at least one detector for detecting the passage of the reaction vessel is arranged on the orbit, and a light source and spectral detection are arranged at a position sandwiching the reaction vessel. In an automatic analyzer for measuring the amount of light transmitted through the reaction vessel by arranging a vessel,
An automatic analyzer that calculates the reaction disk rotation speed based on an output signal of the spectroscopic detector when the reaction disk rotates, and corrects the timing of the start and end of photometry using the calculated speed. A reaction vessel and a reaction vessel detection mechanism are arranged on the circumference of the outer periphery of the reaction disk, and at least one detector for detecting the passage of the reaction vessel is arranged on the orbit, and a light source and spectral detection are arranged at a position sandwiching the reaction vessel. In an automatic analyzer for measuring the amount of light transmitted through the reaction vessel by arranging a vessel,
When the reaction disk rotates, the output signal of the spectroscopic detector is used to detect between the reaction container and the adjacent reaction container, the reaction disk rotation speed is calculated based on the detection result, and the calculated speed is used. An automatic analyzer characterized by correcting the timing of metering start and metering end.

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