JP2009136179A - Method and device for inoculating liquid spawn of mushroom - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for inoculating liquid spawn of mushroom including uniformly inoculating liquid spawn in a wide range of the surface of culture media in a culture container so as to shorten a culturing period and reduce unevenness in culture; and to provide a device for inoculating a liquid spawn of mushroom with which the liquid spawn is uniformly inoculated in a wide range of the surface of culture media in a culture container so as to shorten a culturing period and reduce unevenness in culture. <P>SOLUTION: The method for inoculating a liquid spawn of mushroom includes: feeding liquid mushroom spawn to a liquid spawn-distributing means via a liquid-spawn pressure-feeding means under a prescribed pressure; supplying the liquid spawn of mushroom to a plurality of nozzles S and D arranged on a liquid spawn jetting means under pressure while distributed in a prescribed amount via the liquid spawn-distributing means; and jetting the pressurized liquid spawn of mushroom supplied by the liquid spawn-distributing means through the jetting ports of the nozzles S and D: wherein the liquid spawn of mushroom supplied and pressurized by the liquid spawn-distributing means is jetted in a direction corresponding to the vicinity of the border between the opening part inner wall surface of the container and the surface part of the culture media charged in the culture container through the nozzle S, and in a direction corresponding to the inner part of a plurality of ventilation holes perpendicularly open toward the inner part of the culture media charged in the culture container through the nozzle D so that the liquid spawn is inoculated into the culture media charged in the culture container. The device for inoculating liquid spawn of mushroom is also provided. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

TECHNICAL FIELD The present invention relates to a method and apparatus for inoculating mushroom liquid inoculum, and in particular, liquid inoculum of mushroom inoculated by injecting a liquid mushroom liquid inoculum uniformly from an ejection nozzle over a wider range to a medium contained in a cultivation container. The present invention relates to an inoculation method and apparatus.

The artificial cultivation of mushrooms such as enoki mushrooms, eringi, etc. using the cultivation container, after sterilizing the medium contained in the cultivation container, inoculating the medium in the cultivation container with the mushroom inoculum and cultivating the mushroom cultivation process However, the cultivation process is completed through the growth process, and in the process of inoculating and inoculating mushroom inoculum, it has been conventionally required to shorten the culture period after inoculation as much as possible.

In the conventional inoculum inoculation device, the cause of such a long culture period after inoculation is that most of the inoculated inoculum stays on the surface of the medium, and the rest falls through the vent holes opened in the medium and the bottom of the cultivation container Therefore, inoculation position and inoculation amount of the inoculum in the cultivation container will vary, especially in the center part of the vent hole will be significantly delayed compared to the top and bottom of the culture container, For this reason, the period which the culture | cultivation of the whole cultivation container completes was long. In addition, the cultivation container is filled with a sterilized medium that is opened at the top, and the vicinity of the neck of the cultivation container is the medium surface. In general, a plurality of vent holes are formed in advance in the container in the vertical direction (vertical) for the hypha during the culture period to exchange gas with the outside air.

Therefore, the present inventor, in Patent Document 1, when inserting a container filled with a liquid inoculum of mushrooms into the ventilation hole formed in the culture medium in the cultivation container or when pulling up from the ventilation hole, A method and apparatus for inoculating the liquid inoculum by releasing the liquid inoculum from the container toward the surface of the medium in the middle and near the surface portion is proposed.

However, this inoculation method has a problem that the culture period is prolonged by delaying the culture of the portion of the medium filled in the cultivation container that is in contact with the inner wall surface of the cultivation container. The cause of this problem is that the inoculation method inoculates only the medium inside the vent hole and the surface of the medium, and does not inoculate the medium existing on the inner wall of the cultivation container in contact with the medium. Is started only from the inside of the surface portion of the culture medium and the vent hole, and the culture of the portion of the culture medium surface portion and the inner wall of the cultivation container in contact with the innermost wall of the cultivation container is delayed. In addition, such an inoculation method has a problem that the inoculation apparatus becomes complicated and the manufacturing cost of the apparatus increases. Therefore, the present inventor has further conducted experiments and studies to arrive at the present invention in order to shorten the culture period after inoculation as much as possible.
JP 2004-261134 A

The present invention has been made in view of the above circumstances, and by inoculating the liquid inoculum uniformly over a wider range of the surface of the medium in the cultivation container, the culture period can be shortened and the variation in culture can be reduced. It is an object of the present invention to provide a method and an inoculation apparatus for inoculating liquid mushrooms.

The subject of the present invention is that the liquid inoculum 1 of the mushroom is pumped at a predetermined pressure to the liquid inoculum distributing means 3 by the liquid inoculum pumping means 4, and the liquid inoculated mushroom is disposed in the liquid inoculum injection means 2. The liquid inoculum distributing means 3 distributes and supplies the plurality of S nozzles 11 and D nozzles 12 in a predetermined ratio, and the liquid inoculum of mushrooms supplied and pressurized by the liquid inoculum distributing means 3 From the nozzles of the S nozzle 11 and the D nozzle 12, the S nozzle 11 is the surface portion 41 of the culture medium 45 accommodated in the cultivation container 40, and is in the vicinity of the boundary portion 43 with the opening inner wall surface 42 of the container. Mushroom liquid inoculum is sprayed toward X, and D nozzle 12 is a mushroom liquid inoculum toward the internal direction Y of a plurality of vent holes 46 opened vertically to the inside of the culture medium 45 accommodated in the cultivation container 40. Spray Is solved by a liquid inoculum inoculation method mushrooms, characterized in that the liquid inoculum is inoculated into the medium contained in the culture vessel by.

And the vent hole 46 formed in the inside of the culture medium 45 of the cultivation container 40 used in the present invention is appropriately opened at the central part and / or the outer peripheral part of the culture medium surface 41 of the cultivation container, and the D nozzle 12 is The liquid inoculum injecting means 2 is arranged so as to face the opening position of the vent hole opened in the central portion and / or the outer peripheral portion, and the S nozzle 11 is appropriately placed around the D nozzle 12 in a number of liquid inoculum injecting means 2. And inoculating the liquid inoculum into the medium accommodated in the cultivation container by injecting the liquid inoculum of mushrooms from the respective outlets of the D nozzle 12 and the S nozzle 11.

In addition, an appropriate number of longitudinal grooves 48 are formed on the inner wall surfaces 42 and 47 of the cultivating container 40 from the upper side of the container toward the bottom thereof at the part facing the outlet of the S nozzle 11, and the liquid inoculum is formed. It is characterized by inoculating a medium accommodated in a cultivation container. Furthermore, after the inoculation process of the liquid inoculum to the culture medium accommodated in the cultivation container 40, the aseptic air pressure delivery means 5 passes through the distribution means 3 and reaches all the nozzles arranged in the ejection means 2. An aeration process using aseptic air for discharging residual liquid inoculum is performed on the road and the spout.

Another object of the present invention is to provide a liquid inoculum pumping means 4 for pumping the liquid inoculum 1 of mushrooms to the liquid inoculum distribution means 3 at a predetermined pressure, and the liquid inoculum injection means 2 for feeding the pumped liquid inoculum. Liquid inoculum distribution means 3 that distributes and supplies a plurality of spray nozzles 11 and 12 in a predetermined ratio amount, and a surface portion 41 of a culture medium 45 accommodated in the cultivation container 40, and an inner wall surface 42 of the opening of the container In the vicinity of the boundary 43 between the S nozzle 11 for injecting pressurized liquid inoculum from the jet outlet and the internal direction Y of the plurality of vent holes 46 opened vertically inside the culture medium The mushroom liquid inoculum inoculation device includes the liquid inoculum injection means 2 provided with at least two types of nozzles of the D nozzle 12 that injects the pressurized liquid inoculum from the outlet.

And the ventilation hole formed in the said culture medium 45 of the cultivation container 40 used in this invention opens at least 1 by the center part of the culture medium surface 41 of a cultivation container, and at least 4 by the outer peripheral part of the culture medium surface. A hole, three vent holes opened in the outer periphery of the culture medium surface of the cultivation container, or one ventilation hole selected from one ventilation hole opened in the center of the culture medium surface of the cultivation container, The D nozzle 12 is disposed in the liquid inoculum injection means 2 so as to face the opening position of each vent hole opened in the central portion and / or the outer peripheral portion, and the S nozzle 11 is disposed around the D nozzle 12. It is a mushroom liquid inoculum inoculation device arranged in at least two liquid inoculum injection means 2.

Moreover, the shape of the surface part 41 of the culture medium 45 accommodated in the said cultivation container 40 used in this invention is a mortar shape with which the center part was dented. In addition, an appropriate number of longitudinal grooves 48 are formed on the inner wall surfaces 42 and 47 of the cultivation container 40 from the top to the bottom of the container, and the plurality of longitudinal grooves 48 are the outlets of the S nozzle 11. It is formed in the site | part which opposes.

In addition to the liquid inoculum injection means 2, the liquid inoculum distribution means 3, and the liquid inoculum pressure feeding means 4, the subject of the present invention is distributed to the injection means through the distribution means after the inoculation process of the liquid inoculum is completed. Advantageous by the mushroom liquid inoculum inoculation device provided with aseptic air pumping means 5 for pumping sterile air at a predetermined pressure for discharging residual liquid inoculum to the flow passages and jet outlets that reach all the nozzles installed Solved.

  Furthermore, the subject of the present invention is a plurality of holding means 6 for holding a plurality of the liquid inoculum spraying means 2, the liquid inoculum distributing means 3, the liquid inoculum pressure feeding means 4 and the sterile air pressure feeding means 5, and a plurality of media containing the medium. A mushroom liquid inoculum inoculation device which allows the inoculation of liquid inoculum to the cultivation container at the same time is further advantageously solved.

In the present invention, the liquid inoculum pumping means 4 pumps the mushroom liquid inoculum supplied from the liquid inoculum storage tank or the like to the liquid inoculum distributing means 3 at a predetermined pressure, and the pumped liquid inoculum is distributed in a predetermined ratio amount. Then, from each jet outlet of the plurality of jets S, D nozzles 11, 12, the vicinity of the boundary 43 between the culture medium surface 41 and the inner wall surface 42 of the container opening 43, and the ventilation hole 46 opened into the culture medium 45. The liquid inoculum is inoculated into the medium accommodated in the cultivation container by spraying in the internal direction Y.

According to the present invention having such a configuration, the liquid inoculum adheres to the inner peripheral portion and the bottom portion of the vent hole 46 by the injection of the D nozzle 12, thereby inoculating the medium on the inner peripheral portion and the bottom portion of the vent hole. In addition to (inoculation of air holes), the liquid inoculum injected toward the boundary vicinity 43 by the injection of the S nozzle 11 flows into the gap existing in the vicinity of the boundary, and the liquid inoculum is in the medium container. It inoculates to the culture medium 45 which exists in the wall surfaces 42 and 47 vicinity (side wall inoculation). Further, the liquid inoculum that has not completely entered the gap in the boundary vicinity portion 43 spreads on the medium surface portion 41 and is inoculated with the medium surface portion (surface inoculation).

In addition, since the culture medium accommodated in a cultivation container is comprised with the coarse particle, the clearance gap which can pass a liquid originally exists in the culture medium which exists in the part which contacts the inner wall of a cultivation container. The weight of the medium filled in a standard size cultivation container generally used is 610 to 620 g. Even in this case, the gap exists, but in the present invention, the weight of the medium to be filled is around 580 g. The effect of the side wall inoculation can be enhanced by using the (lightly packed) cultivation container.

Thus, in the present invention, the inoculation process of the liquid inoculum is also performed on the side wall inoculation region c. Therefore, conventionally, inoculation treatment of the liquid inoculum is performed only on a part of the surface inoculation area a and the ventilation hole inoculation area b, and the mycelial growth started from these areas is the side wall inoculation area c in the present invention. Therefore, it is started from the three areas of the inoculation areas a, b, and c, and the effect of greatly shortening the culture period is obtained.

  In the present invention, since the D nozzle 12 is disposed in the liquid inoculum injection means 2 so as to face the opening position of each vent hole 46, the inoculation process of the liquid inoculum in the vent hole inoculation area b is ensured. Since the shape of the medium surface portion 41 is formed in a mortar having a recessed central portion, the liquid inoculum that has not flowed into the gap existing in the vicinity of the boundary portion 43 diffuses uniformly and over a wide range on the medium surface portion 41. As a result, the entire surface of the medium surface portion 41 is uniformly inoculated, and variations in culture are reduced.

  Furthermore, in the present invention, an appropriate number of longitudinal grooves 48 are formed on the inner wall surface of the cultivation container 40 from the top toward the bottom, so that the longitudinal grooves 48 are between the medium 45 and the inner wall surface of the cultivation container 40. A gap is formed and functions as a flow path for the liquid inoculum, and the liquid inoculum sprayed from the S nozzle 11 flows into the vertical groove 48 to reach the lower part of the container. As a result, the liquid inoculum is inoculated into a wider part of the medium container interface 47, leading to a further shortening of the culture period.

  Further, in the present invention, after the inoculation treatment of the liquid inoculum on the culture medium, the flow passages and the jet outlets which reach all the nozzles arranged in the jetting means 2 through the distributing means 3 by the sterile air pressure feeding means 5 On the other hand, since it is configured to carry out aeration treatment with sterile air for discharging the residual liquid inoculum, the liquid inoculum is not dripped from each nozzle until the next inoculation process, and the inoculation device It is kept clean and can prevent the propagation of germs from the liquid dripping portion, and can prevent the flow passage and the spout from getting stuck.

Further, in the present invention, since the liquid inoculum spraying means 2, the liquid inoculum distributing means 3, the liquid inoculum pressure feeding means 4 and the holding means 6 for holding a plurality of aseptic air pressure feeding means 5 are provided, a plurality of media containing the culture medium are provided. The inoculation process of the liquid inoculum can be simultaneously performed on the cultivation container. In the present invention, the number of vent holes, the number of S nozzles, the number of D nozzles, and the arrangement position can be optimally changed according to the type of mushrooms to be cultivated, the size and shape of the cultivation container.

Hereinafter, embodiments of the liquid inoculation method and the inoculation apparatus for mushrooms according to the present invention will be disclosed with reference to the accompanying drawings. FIG. 1 is a flowchart showing a processing procedure of a liquid inoculum method for mushrooms according to the present invention, and FIG. 2 is a schematic cross-sectional view showing a configuration example of a liquid inoculum apparatus for mushrooms according to the present invention. With reference to FIG. 2, the structure of the cultivation container used for the liquid inoculum inoculation apparatus for implementing the liquid inoculum method of the mushroom which concerns on this invention previously, and this inoculation apparatus is demonstrated.

As shown in FIG. 2, the liquid inoculum inoculation device according to the present invention includes a liquid inoculum pumping means 4 for pumping the liquid inoculum 1 of mushrooms to the liquid inoculum distribution means 3 at a predetermined pressure, and a liquid inoculum of the pumped mushrooms in liquid form. Liquid inoculum having a plurality of S nozzles 11 and D nozzles 12 for inoculating the inoculum of liquid inoculum by injecting the liquid inoculum into the culture medium stored in the cultivation container. The inoculum injection means 2 is configured. Moreover, the inoculation apparatus according to the present invention reaches all the nozzles arranged in the ejection unit 2 through the distribution unit 3 after the inoculation process of the liquid inoculum on the medium accommodated in the cultivation container 40. A sterile air pumping means 5 for performing aeration treatment with sterile air for discharging residual liquid inoculum for the flow passage and the spout is provided, and at the same time for a plurality of cultivation containers containing the culture medium. In order to enable inoculation of the liquid inoculum, the liquid inoculum injection means 2, the liquid inoculum distribution means 3, the liquid inoculum pressure feeding means 4, and the holding means 6 for holding a plurality of aseptic air pressure feeding means 5 can be provided ( (See FIG. 9).

And the cultivation container 40 used for the inoculation apparatus which concerns on this invention is filled with the culture medium 45 by which upper direction was opened and sterilized inside as shown in FIG. 2, FIG. The vicinity of the neck (opening) is the medium surface 41. In addition, a plurality of vent holes 46 for exchanging the mycelium during the culture period with the outside air are vertically opened from the medium surface 41 toward the bottom in the medium accommodated in the container. In the figure, 42 is the inner wall surface of the opening of the cultivation container, 43 is the vicinity of the boundary between the medium surface portion 41 and the opening inner wall surface 42, 45 is the medium, 46 is a vent hole, and 47 is the cultivation container. The inner wall surface of the torso is shown. 48 has shown the vertical groove formed in the inner wall face of the cultivation container mentioned later (refer FIGS. 5-8). Further, X indicates the liquid inoculum injection direction of the S nozzle 11, and Y indicates the liquid inoculum injection direction of the D nozzle 12. Moreover, the part a which attached | subjected the vertical line shows the inoculation area | region of the culture medium surface part 41, the part b attached | subjected the horizontal line shows the inoculation area | region of the vent hole 46, and the part c attached | subjected with the dot inoculate the inner wall surface part of a cultivation container Indicates the area. Moreover, the culture-medium surface part 41 forms the mortar shape where the center part was dented here.

Next, based on the configuration of the liquid inoculum inoculation device according to the present invention and the cultivation container used in the inoculation device, the processing procedure of the mushroom liquid inoculum inoculation method according to the present invention will be described with reference to FIG. . In the present invention, first, the liquid inoculum of the mushroom supplied from the liquid inoculum storage tank or the like is pumped by the liquid inoculum pressurizing means 4 to the liquid inoculum distributing means 3 at a predetermined pressure. The liquid inoculum fed by pressure is distributed and supplied to the plurality of S nozzles 11 and D nozzles 12 arranged in the liquid inoculum injection means 2 by the liquid inoculum distribution means 3 (step S1). The liquid inoculum supplied and pressurized from the liquid inoculum distributing means 3 is directed to the vicinity of the boundary from the outlet of the S nozzle 11 toward the boundary near portion 43 of the culture medium surface 41 and the inner wall surface 42 of the opening of the container. Fan-shaped so as to expand (step S2), from the outlet of the D nozzle 12 toward the inner direction Y of the vent hole 46 opened in the culture medium 45, fan-shaped so as to contact the inner peripheral portion of the vent hole, It is injected in a conical shape (step S3).

In the present invention, a culture container having a weight of about 580 g of the medium to be filled (lightly packed) is used, so the boundary vicinity 43 and the medium, which are the parts where the medium on the surface and the inner wall surface of the container are in contact with each other, There is a gap through which the liquid inoculum can pass in the medium container boundary surfaces 42 and 47 with which the inner wall surface of the cultivation container contacts. Therefore, the liquid inoculum sprayed from the S nozzle 11 toward the boundary vicinity portion 43 direction X in step S <b> 2 flows into the gap of the medium existing in the boundary line vicinity portion 43. The liquid inoculum that has flowed into the gap in the vicinity of the boundary 43 sews down the gap existing in the medium container boundary surfaces 42 and 47 and flows down along the inner wall surface of the cultivation container 40 (step S4). The liquid inoculum is inoculated (inoculated to the side wall inoculation area c) by the liquid inoculum flowing down along the inner wall surface of the cultivation container 40 with the medium in the vicinity of the medium container boundary surface. (Step S7). In addition, even if it uses the cultivation container which made the weight of the culture medium to fill about 610-620g (medium packing), since the culture medium is comprised with the coarse particle, the clearance gap which can pass a fluid exists. In the present invention, in order to more efficiently inoculate the culture medium at the boundary surface of the culture medium container, a number of vertical grooves 48 described later are appropriately formed on the inner wall surface of the cultivation container 40 from the top to the bottom.

Of the liquid inoculum sprayed from the S nozzle 11 toward the boundary vicinity portion 43 direction X in step S2, the liquid inoculum that has not completely entered the gap in the boundary vicinity portion 43 diffuses on the medium surface portion 41 (step S5). . As the liquid inoculum diffuses into the medium surface part 41, the medium on the medium surface part 41 is inoculated (inoculated against the surface inoculation area a) (step S8).

In the present invention, the shape of the medium surface portion 41 is formed in a mortar shape having a recessed central portion. By forming the culture medium surface portion 41 in a mortar shape with a recessed central portion, among the liquid inoculum sprayed from the S nozzle 11, the liquid inoculum that has not flowed into the gap existing in the borderline vicinity portion 43 is transferred to the culture medium surface portion 41. It spreads more uniformly and over a wide area up to the center. Thereby, the whole surface of the culture medium surface part 41 is inoculated uniformly, and the dispersion | variation in culture | cultivation is reduced.

On the other hand, the liquid inoculum 1 ejected from the D nozzle 12 toward the inner direction Y of the vent hole 46 opened in the medium in Step S3 adheres to the inner peripheral portion and the bottom portion of the vent hole 46 (Step S6). The attached liquid inoculum is inoculated (inoculated into the vent hole inoculation area b) into the medium at the inner periphery and bottom of the vent hole 46 (step S9). When the injection of a predetermined amount of liquid inoculum by the S nozzle 11 and D nozzle 12 is completed, the supply of the liquid inoculum from the liquid inoculum pumping means 4 is stopped, and the liquid inoculum inoculation process is completed (step S10).

Side wall inoculation area c and culture medium surface, which is an inoculation area in the vicinity of medium container inner wall surface 47, by S nozzle 11 that injects liquid inoculum toward direction X in the vicinity of boundary 43 between medium surface part 41 and inner wall surface 42 of the opening of the container. A liquid inoculum is inoculated with respect to the culture medium of the surface inoculation area | region a which is the inoculation area | region of the part 41. FIG. Further, the D nozzle 12 that injects the liquid inoculum toward the inner direction Y of the vent hole 46 opened inside the medium causes the medium in the vent hole inoculation region b that is the inoculation region on the inner peripheral surface portion and the bottom portion of the vent hole 46. On the other hand, liquid inoculum is inoculated. As a result, the growth of mycelia that has been started from only a part of the inoculation area a and the inoculation area b is started from the three inoculation areas a, b, and c, and the culture period is greatly shortened. Effect is obtained.

After the inoculation process of the liquid inoculum is completed, aseptic air is supplied from the air pumping means 5 to the liquid inoculum distributing means 3, and aseptic air pressurized from the liquid inoculum distributing means 3 is supplied to the liquid inoculum injection means 2 Is done. The liquid inoculum remaining in the liquid inoculum spraying means 2 provided with the liquid inoculum distributing means 3 and the S nozzle and the D nozzle is discharged from the S nozzle and the D nozzle by aseptic air fed from the air pressure feeding means 5 (step). S11). By this aeration process (liquid inoculum discharge process) with aseptic air, the liquid inoculum remaining in the flow passages and jet outlets that reach all the nozzles arranged in the injection unit 2 through the distribution unit 3 is surely discharged. .

This aeration process is to cover the cultivation container after inoculation with the liquid inoculum, but it prevents the bacteria from being generated in the container lid, container storage case, etc. due to liquid dripping from the nozzle. This is done to ensure a clean and clean environment and to prevent the flow passages and spouts from getting stuck. If it is determined that the aeration process is not necessary depending on the structure of the flow path and the jet outlet, this process may be omitted. After this aeration process is completed, the process proceeds to the next inoculation process.

FIG. 3 is a view showing a state in which the liquid inoculum is ejected from the S nozzle 11 (1, 2) and the D nozzle 12 (3, 4, 5, 6). The S nozzle 11 and the D nozzle 12 are arranged in the liquid inoculum injection means 2 and are arranged above the opening of the cultivation container at the time of inoculation. The D nozzle 12 that injects the liquid inoculum toward the inner direction Y of the vent hole 46 is disposed in the liquid inoculum injection means 2 so as to face the opening position of each vent hole in order to surely inject the air into the vent hole. , S nozzles 11 are appropriately arranged around the D nozzle 12. The S nozzle 11 aims at injecting the liquid inoculum by aiming at the boundary vicinity 43 direction X and inoculating the medium in the side wall inoculation area c and the surface inoculation area a. It is convenient to use the nozzle (1) for injection so as to have a wide angle in the injection direction. The S nozzle 11 is not particularly limited as long as the straight nozzle (2) is used to inject the nozzle into a linear shape, a cylindrical shape, a quadrangular prism shape, or the like.

The D nozzle 12 aims to inoculate the culture medium in the ventilation hole inoculation area b by injecting the liquid inoculum aiming at the internal direction Y of each ventilation hole 46 opened inside the culture medium. Using a fan-shaped nozzle (3), a full cone nozzle (4), and an empty cone nozzle (5) in the downward direction, a wide angle is formed in the injection direction so that liquid inoculum adheres to the inner peripheral wall of the vent hole. It is convenient to inject like this. The D nozzle 12 may be sprayed in a linear shape, a cylindrical shape, a quadrangular prism shape or the like toward the inner peripheral wall of the ventilation hole by using a straight nozzle (6), and is not particularly limited.

FIG. 4 is a plan view of the cultivation container containing the culture medium as viewed from the container opening side. The cultivation container 40 used here is a container generally used for cultivation of enoki mushrooms, and is a cultivation container having an opening having a diameter of about 65 mm. As shown in the figure, one vent hole 46 is opened in the central part of the medium surface part 41 exposed at the opening part of the cultivation container 40 and four in the outer peripheral part approaching the inner wall surface 42 of the container opening part. . By disposing the vent hole 46 close to the inner wall surface 42 of the opening, the vent hole inoculation area b and the side wall inoculation area c are separated, so that the culture medium accommodated in the cultivation container is more uniformly inoculated. As a result, the variation in culture is reduced, and as a result, the effect of shortening the culture period is further improved.

FIG. 5 is a plan view for explaining the arrangement relationship between the S nozzle 11 and the D nozzle 12 arranged in the liquid inoculum injection means 2 in correspondence with the opening position of the vent hole 46 shown in FIG. As shown in FIG. 5, the D nozzle 12 indicated by a circle with a vertical line added above one vent hole opened in the center of the medium surface portion 41 and above the four vent holes opened in the outer peripheral portion. Further, they are arranged so as to face the respective vent holes. Four S nozzles 11 indicated by circles with dots added are arranged around the D nozzle. The outlet of the S nozzle 11 is disposed so as to face the boundary vicinity portion 43, and injects the liquid inoculum toward the direction X indicated by the arrow. In addition, the vertical groove 48 mentioned later is formed in the boundary vicinity part 43 of the inner wall surface 42 of a cultivation container.

FIG. 6 illustrates the positional relationship between the S nozzle 11 and the D nozzle 12 disposed in the liquid inoculum injection means 2 corresponding to the opening position of the vent hole according to another embodiment of the embodiment shown in FIG. FIG. The container used here is a thin cultivated container 50 having a container body diameter of about 78 mm, and the number of vent holes is smaller than that of generally used containers. As shown in the drawing, three vent holes 46 are opened along the inner wall surface 42 of the medium surface portion 41 exposed at the opening of the small-diameter cultivation container 50. The D nozzle 12 indicated by a circle with a vertical line is arranged above the three vent holes opened along the inner wall surface 42 so as to face the vent holes. Three S nozzles 11 indicated by circles with dots added are arranged inside the D nozzle.

Thus, in this invention, according to the size of the cultivation container to be used, a shape, the opening position of a ventilation hole, and the number of ventilation holes, it can change optimally the arrangement position and quantity of S nozzle and D nozzle suitably. Is possible. As in the above-described embodiment, the jet nozzle of the S nozzle 11 is disposed so as to face the boundary vicinity portion 43 and injects the liquid inoculum toward the direction X indicated by the arrow. Further, a longitudinal groove 48 described later is formed in the boundary vicinity 43 of the inner wall surface 42 of the cultivation container.

FIG. 7 shows the arrangement of the S nozzle 11 and the D nozzle 12 arranged in the liquid inoculum injection means 2 corresponding to the opening position of the vent hole according to still another embodiment of the embodiment shown in FIGS. It is a top view for demonstrating a relationship. The cultivation container 60 used here is a container generally used for cultivation of eringi. As shown in the figure, one vent hole 46 is opened at the center of the culture medium surface 41 exposed at the opening of the cultivation container 60 for eringi. A D-nozzle 12 indicated by a circle with a vertical line is arranged above one vent hole opened in the center portion and opposed to the vent hole. Four S nozzles 11 indicated by circles with dots added are arranged on the outer periphery of the D nozzle.

As in the above-described embodiment, the jet nozzle of the S nozzle 11 is disposed so as to face the boundary vicinity portion 43 and injects the liquid inoculum toward the direction X indicated by the arrow. Further, a longitudinal groove 48 described later is formed in the boundary vicinity 43 of the inner wall surface 42 of the cultivation container. As described above, in the present invention, depending on the size and shape of the cultivation container adapted to the type of straw to be cultivated, the opening position of the ventilation holes, and the number of ventilation holes, the arrangement position and quantity of the S nozzle and D nozzle are appropriately set. It is possible to change optimally.

FIG. 8 is a view for explaining the longitudinal grooves 48 formed in the inner wall surfaces 42 and 47 of the cultivation container used in the liquid inoculation device for mushrooms according to the present invention. As shown in the figure, a longitudinal groove 48 is formed from a position higher than the medium surface portion 41 to an arbitrary position at the bottom of the cultivation container 40. The longitudinal groove 48 only needs to function as a passage for allowing the liquid inoculum sprayed from the S nozzle 11 toward the boundary vicinity 43 direction X to pass to the bottom of the container. Here, the longitudinal groove is opposed to the ejection outlet of each S nozzle 11. However, the arrangement position, number, depth, shape, and the like of the longitudinal grooves are not particularly limited, and for example, several fine grooves can be formed.

Further, as shown in FIGS. 5, 6, and 7, the vertical groove 48 is formed in a direction X in which the S nozzle 11 ejects the liquid inoculum, that is, in a portion facing the jet nozzle of the S nozzle 11 on the inner wall surface 42. convenient. By forming the vertical groove 48 at this position, most of the liquid inoculum ejected from the S nozzle 11 flows into the flow path formed by the vertical groove and reaches further below the container. When the liquid inoculum 1 reaches below, the liquid inoculum is inoculated into a wider part of the medium container boundary surface 47, and the culture period is further shortened. In addition, the formation position of this vertical groove 48 is not limited only to the site | part which opposes the spout of S nozzle 11, Arbitrary numbers may be formed in the vicinity and the appropriate position of a cultivation container inner wall surface.

FIG. 9 is a view showing a state in which a plurality of cultivation containers are simultaneously inoculated with liquid inoculum by the holding means of the mushroom liquid inoculum inoculation device according to the present invention. As shown in the figure, in the present invention, the liquid inoculum injection means 2, the liquid inoculum distribution means 3, the liquid inoculum pressure feeding means 4, and the sterile air pressure feeding means 5 (all means are also referred to as “liquid inoculum inoculation device”). Is provided with a holding means 6 for holding a plurality. The holding means 6 holds a plurality of liquid inoculum inoculation devices at predetermined positions. Here, the inoculation of liquid inoculum is enabled simultaneously with respect to the four cultivation containers 40 in which the culture medium is accommodated. When the inoculation and aeration process for the four cultivation containers are completed, the inoculation and aeration process for the four cultivation containers arranged in the next row are carried out. Can be shortened. In addition, although the holding means which hold | maintains four liquid inoculum inoculation apparatuses is shown here, the liquid inoculum inoculation apparatus to hold | maintain is not restricted to this, The liquid inoculum inoculation apparatus of 8, 16, 16, etc. is used. It can be configured to hold.

A cultivation container in which five vent holes are formed in the medium at the position shown in FIG. 5, and an inoculation apparatus in which four S nozzles and five D nozzles are arranged above the vent holes as shown in FIG. Inoculation treatment of enoki mushroom inoculum was carried out. The cultivation container has a capacity of 850 cc and an opening diameter of 65 mm. The cultivation container is filled with a medium containing 580 g of corn cob as a main component (lightly packed), and totals 2 cc each toward the inside of the five vent holes. 10 cc liquid inoculum of enoki mushroom was sprayed from D nozzle, and a total of 8 cc liquid inoculum of enoki mushroom was sprayed from S nozzle to the boundary between the cultivation container and the medium surface. As a result, in the inoculation treatment by the conventional method of inoculating the inside of the vent hole and the surface of the medium (the inoculation method and apparatus disclosed in Japanese Patent Application Laid-Open No. 2004-261134), the culture period that took 19 to 21 days was Shortened to 16-18 days.

One from the top to the bottom of the container in the portion of the cultivation container used in Example 1 that faces the S nozzle spout of the inner wall of the container (the X direction in which the S nozzle injects liquid inoculum). The container in which the vertical grooves were formed was filled with the culture medium, and the inoculation process of enoki mushrooms was carried out in the same manner as in Example 1 using the inoculation apparatus used in Example 1. As a result, the culture period was significantly shortened from the culture period in Example 1 to 14 to 15 days.

The liquid inoculum inoculation method and apparatus of the mushroom according to the present invention are applied to the culture medium in the inoculation area (side wall inoculation area c) near the inner wall surface of the medium container and the inoculation area (surface inoculation area a) of the medium surface by injection of the S nozzle. Inoculate the liquid inoculum and inoculate the medium of the inoculation area (ventilation area b) on the inner peripheral surface and bottom of the vent hole by spraying the D nozzle. Therefore, since the inoculum is uniformly inoculated in a wide range of the medium contained in the cultivation container, the culture period can be greatly shortened, and the variation in culture can be further reduced.

In the present invention, after the inoculation process of the liquid inoculum is completed, an aeration process (residual liquid inoculum discharge process) for removing the liquid inoculum remaining in the flow path of the liquid inoculum and the nozzle outlet is performed. Therefore, the liquid inoculum does not drip from each nozzle until the next inoculation process, the inoculation device is kept clean, and the propagation of miscellaneous bacteria from the liquid dripping part can be prevented and the risk of contamination during inoculation is reduced. can do. Further, in the present invention, the number of nozzles and the arrangement position can be optimally changed according to the type of mushrooms to be cultivated and the size and shape of the cultivation container to be used, so that it is possible to inoculate various mushroom inoculums Become.

It is a flowchart which shows the process sequence of the liquid inoculation method of the mushroom which concerns on this invention. It is a schematic sectional drawing which shows the structural example of the liquid inoculation apparatus of the mushroom which concerns on this invention. It is a perspective view for demonstrating the injection state of the liquid inoculum of the nozzle of the liquid inoculum apparatus of the mushroom which concerns on this invention. It is a top view for demonstrating the opening position of the ventilation hole opened in the culture medium of the cultivation container used for the liquid inoculation apparatus of the mushroom which concerns on this invention. It is a top view for demonstrating the opening position of the ventilation hole opened in the culture medium of the cultivation container used for the liquid inoculation apparatus of the mushroom which concerns on this invention, the arrangement | positioning relationship of a nozzle, and the formation position of a vertical groove. It is a top view for demonstrating the opening relationship of the ventilation hole which concerns on the other Example of the Example shown in FIG. 5, the arrangement | positioning relationship of a nozzle, and the position of a vertical groove. It is a top view for demonstrating the opening position of the vent hole which concerns on further Example of the Example shown in FIG. 5, the arrangement | positioning relationship of a nozzle, and the formation position of a vertical groove. It is a figure for demonstrating the vertical groove | channel formed in the cultivation container inner wall surface of the liquid inoculation apparatus of the mushroom which concerns on this invention. It is a figure for demonstrating the state which inoculates a liquid inoculum with respect to several cultivation containers by the holding means of the liquid inoculum inoculation apparatus of the mushroom which concerns on this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Liquid inoculum 2 Liquid inoculum injection means 3 Liquid inoculum distribution means 4 Liquid inoculum pressurization means 5 Aseptic air pumping means 6 Holding means 11 S nozzle (nozzle which injects liquid inoculum toward direction X)
12 D nozzle (nozzle that injects liquid inoculum toward direction Y)
40 Cultivation container 41 Medium surface part 42 Cultivation container opening inner wall surface 43 Boundary vicinity part 45 Medium 46 Aeration hole 47 Cylinder container inner wall surface (medium container boundary surface)
48 Longitudinal groove 50 Small-diameter cultivation container 60 Eringi cultivation container X, Y Spray direction a liquid inoculum a Surface inoculation area b Ventilation hole inoculation area c Side wall inoculation area

Claims (11)

The liquid inoculum of the mushroom is pumped at a predetermined pressure to the liquid inoculum distributing means by the liquid inoculum pumping means
Supplying the liquid inoculum of the pumped mushroom to a plurality of S nozzles and D nozzles arranged in the liquid inoculum injection means in a predetermined ratio amount by the liquid inoculum distribution means;
The mushroom liquid inoculum supplied and pressurized by the liquid inoculum distributing means is discharged from each of the nozzles of the plurality of S nozzles and D nozzles.
S nozzle is a surface portion of the medium accommodated in the cultivation container, and sprays the liquid inoculum of mushrooms toward the vicinity of the boundary with the inner wall surface of the opening of the container,
D nozzle sprays the liquid inoculum of mushrooms toward the inner direction of a plurality of vent holes opened vertically inside the culture medium accommodated in the cultivation container,
A method for inoculating liquid inoculum of mushrooms, comprising inoculating a liquid inoculum into a medium accommodated in a cultivation container.   A number of the vent holes are appropriately opened at the center and / or the outer periphery of the culture medium surface of the cultivation container, and the D nozzle is opposed to the opening position of the vent hole opened at the center and / or the outer periphery. By disposing in the liquid inoculum injection means, arranging the S nozzle as appropriate in the vicinity of the D nozzle in several liquid inoculum injection means, and injecting the liquid inoculum of mushrooms from the respective outlets of the D nozzle and S nozzle The method for inoculating a liquid inoculum of mushrooms according to claim 1, wherein the inoculum of the liquid inoculum is carried out on a medium accommodated in a cultivation container.   A medium in which the liquid inoculum is accommodated in the cultivation container by forming an appropriate number of longitudinal grooves from the upper part of the container toward the bottom on the inner wall surface of the cultivation container and facing the spout of the S nozzle. The method for inoculating a liquid mushroom inoculum according to claim 1 or 2, wherein   After the inoculation treatment of the liquid inoculum on the medium accommodated in the cultivation container, by the aseptic air pumping means, the flow path leading to all the nozzles arranged in the jetting means through the distributing means, the jet outlet 4. A method for inoculating a liquid inoculum of a mushroom according to any one of claims 1 to 3, wherein an aeration process using aseptic air for discharging the residual liquid inoculum is performed. Liquid inoculum pumping means for pumping liquid inoculum of mushrooms to the liquid inoculum distribution means at a predetermined pressure;
Liquid inoculum distribution means for distributing and supplying the pumped liquid inoculum to a plurality of injection nozzles S, D arranged in the liquid inoculum injection means in a predetermined ratio amount;
An S nozzle for injecting a pressurized liquid inoculum from a jet outlet toward the vicinity of the boundary between the surface of the medium contained in the cultivation container and the opening inner wall surface of the container, and perpendicular to the inside of the medium Liquid inoculum injecting means comprising at least two types of nozzles, D nozzles, for injecting liquid inoculum pressurized toward the inside of the plurality of vent holes opened in
An inoculation device for liquid inoculum of mushrooms, comprising:   At least one vent hole formed in the medium inside the culture medium surface and at least four vent holes opened in the outer periphery of the culture medium surface, and three ventilation holes formed in the culture container outer periphery of the culture container. Any one of the vent holes selected from the vent hole to be opened or one vent hole to be opened at the center part of the culture medium surface of the cultivation container, and the D nozzle is provided at the center part and / or the outer peripheral part. The liquid inoculum spraying means is disposed so as to face the opening position of each opened vent, and at least two liquid inoculum spraying means are disposed in the periphery of the D nozzle. The apparatus for inoculating liquid inoculum of mushrooms according to claim 5.   The liquid seed inoculation device for mushrooms according to any one of claims 5 and 6, wherein the surface portion of the culture medium accommodated in the cultivation container forms a mortar shape with a recessed central portion.   The mushroom liquid inoculum inoculation device according to any one of claims 5 to 7, wherein an appropriate number of longitudinal grooves are formed on the inner wall surface of the cultivation container from the top to the bottom of the container.   9. The mushroom liquid inoculum inoculation device according to claim 8, wherein the plurality of longitudinal grooves are formed in a portion facing the jet nozzle of the S nozzle.   In addition to the liquid inoculum injection means, the liquid inoculum distribution means, and the liquid inoculum pressure feeding means according to claim 5, after the inoculation process of the liquid inoculum is completed, all of the liquid inoculum disposed in the injection means through the distribution means The sterilized air pumping means for pumping sterilized air at a predetermined pressure for discharging the residual liquid inoculum to the flow passage leading to the nozzle and the jet outlet is provided. The liquid inoculum inoculation device of the mushroom as described.   A liquid inoculum spraying means, a liquid inoculum distribution means, a liquid inoculum pressure feeding means, and a plurality of aseptic air pressure feeding means as claimed in claim 5, comprising a holding means for holding a plurality of culture containers containing a culture medium. The liquid inoculum inoculation device for mushrooms according to any one of claims 5 to 10, wherein the inoculation of liquid inoculum is enabled at the same time.

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