JP2009097965A - Specimen transfer device to nuclear magnetic resonance device and nuclear magnetic resonance device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a specimen transfer device and an NMR device wherein a problem is solved that specimen molecules in a specimen solution diffuse up to an extrusion solvent to lower the concentration of the specimen molecules in a detection space, the problem being an inadequacy in a technique for transporting a specimen solution to a detection space of the NMR device by using an extrusion solvent, and also a problem is solved that it takes time to transfer a specimen solution, the problem being an inadequacy in a method for transferring the specimen solution by using gas. <P>SOLUTION: A movable wall is provided across a transfer duct for the specimen solution. The specimen solution is transferred to the detection space by means of the extrusion solvent with the movable wall between. Further, a stopper preventing the movable wall from moving is provided in the specimen transfer channel in order that the specimen solution is accurately transferred to the detection space. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a sample transfer apparatus for transferring a liquid sample to a detection space of a nuclear magnetic resonance apparatus, and also relates to a nuclear magnetic resonance apparatus provided with the sample transfer apparatus.

  The Nuclear Magnetic Resonance (hereinafter referred to as NMR) apparatus irradiates a sample molecule placed in a detection space in a magnet that creates a strong static magnetic field with a series of high-frequency alternating magnetic field patterns, thereby causing nuclear spins in the sample molecule to be irradiated. Is a device for measuring the response of the nuclear spin. By analyzing the nuclear spin response to various AC magnetic field patterns, the molecular structure, molecular dynamics, and intermolecular interactions can be analyzed. An analysis method using an NMR apparatus has features that are not found in other analysis methods in that it does not require destruction of sample molecules and does not require crystallization of sample molecules.

  The above features of the NMR analysis technique are particularly suitable for drug discovery research. In drug discovery research, it is preferable to measure the interaction between proteins and drugs that constitute the human body in an environment close to the human body. The NMR analysis method can be measured by preparing a sample solution in which a sample molecule of a protein is dissolved in a sample solvent close to the human body environment, for example, water having a adjusted salt concentration, and putting a drug into this sample solution. Moreover, since the protein used for NMR measurement is not destroyed, it can be recovered and reused. This point is advantageous in measurement using expensive and difficult to obtain proteins as sample molecules.

  As a method for increasing the efficiency of NMR measurement using a sample solution, a flow NMR (flow NMR) apparatus has been devised. For example, in Patent Document 1, a sample transfer channel connected from the outside of a magnet to a detection space in the magnet is formed, a sample solution is injected into the channel from the outside of the magnet, and an extrusion solvent is further injected into the sample solution. A flow NMR apparatus is disclosed that transports the gas to the detection space.

  However, the method of transferring the sample to the detection space using the extrusion solvent has a problem that the sample molecules in the sample solution diffuse to the extrusion solvent and the concentration of the sample molecules in the detection space decreases. When the sample molecule concentration in the detection space decreases, the response signal detected by the NMR apparatus becomes weak, and the error of the analysis result increases.

  Patent Document 2 discloses a technique for solving the problem of sample molecules diffusing into an extrusion solvent by using a gas for extrusion of a sample solution. According to Patent Document 2, two gas pressure sources are provided in front of and behind the detection space of the sample transfer channel, and the pressure difference between the two gas pressure sources is adjusted, so that the sample solution is moved to the detection space. Be transported.

JP-T-2004-534958 Japanese Patent Laid-Open No. 10-90383

  However, the method of using gas for transferring the sample solution has a problem in that it takes time to transfer. One of the reasons why it takes a long time to transfer the gas is that the compressibility of the gas is much higher than that of the liquid and it is difficult to accurately determine the position of the sample solution by the gas pressure.

  In the NMR apparatus, accurately positioning the sample solution in the detection space is important in terms of both the intensity of the response signal and the resolution of the signal. The intensity of the response signal is maximized when all sample molecules in the sample solution are present in the predetermined detection space of the detection antenna. If even a part of the sample solution deviates from the detection space, the response signal intensity decreases. The resolution of the response signal is best when the static magnetic field strength in the sample solution is uniform. Since the magnet apparatus of the NMR apparatus is manufactured so as to create the most uniform static magnetic field in the detection space, the best signal resolution can be obtained when the sample solution is accurately positioned in the detection space.

  In the prior art of Patent Document 2, in order to accurately position the sample solution in the detection space, a sensor that detects the position of the sample solution along the flow path and a signal from the sensor are processed to adjust the gas pressure source. A signal processor is provided. However, because of the high compression ratio of the gas, it is necessary to repeatedly detect the signal and adjust the gas pressure source several times, and it takes time to position the sample solution in the detection space.

  Another reason that it takes time to transfer the sample solution when using gas is that the gas pressure cannot be set high.

  When the gas pressure is increased, the interface between the gas and the sample solution becomes unstable, and gas bubbles are generated in the sample solution. Foam reduces the sample volume in the detection space and weakens the signal intensity. Also, magnetization is generated along the bubble surface due to the difference in magnetic susceptibility between the gas and the sample solution, reducing the static magnetic field uniformity in the detection space and degrading the signal resolution. Furthermore, when the pressure of the gas is increased, the gas is dissolved in the sample solution, causing a problem that the energy dissipation of the sample molecules is accelerated. When the energy dissipation of the sample molecules increases, the signal decays faster, which causes an error in analysis.

  It takes a long time to transfer the sample solution, especially in the case of NMR measurement related to molecular dynamics and interaction between molecules.

  An example of molecular dynamics to be measured by NMR is protein folding. Protein folding is a process in which a protein consisting of a one-dimensional peptide chain is folded into a unique three-dimensional structure. The relationship between the order of peptides that constitute a protein and the three-dimensional structure of the protein It is important to investigate.

  Another example in which molecular dynamics is related to NMR measurement is measurement using a dynamic nuclear polarization (hereinafter DNP) phenomenon. DNP is a phenomenon in which when an electron spin whose magnetic susceptibility is several million times higher than that of a nuclear spin is magnetized, the magnetization of the electron spin is transferred to the nuclear spin by the interaction between the nuclear spin and the electron spin. There is a report that the nuclear spin magnetization temporarily reaches 10,000 times or more from the thermal equilibrium state by DNP, and the intensity of the NMR signal is improved by 10,000 times or more. However, the magnetization of the nuclear spin by DNP is temporary and returns to the value of the thermal equilibrium state with time. Therefore, in order to utilize the effect of improving the NMR signal intensity by DNP, it is important to shorten the time elapsed from the occurrence of the DNP phenomenon until the NMR measurement is performed.

  As described above, in the conventional method using an extrusion solvent for transferring a sample solution, there is a problem that sample molecules in the sample solution diffuse to the extrusion solvent. On the other hand, in the method using gas, it takes time to transfer the sample. There's a problem.

  The purpose of the present invention is to improve the technology for transferring the sample solution to the detection space of the NMR apparatus using the extrusion solvent, so that the sample molecules in the sample solution diffuse to the extrusion solvent and the concentration of the sample molecules in the detection space is reduced. An object of the present invention is to provide a sample transfer apparatus and an NMR apparatus that solve the problem of lowering and solve the problem that the transfer takes time, which is a problem of the method of transferring a sample solution using a gas.

  In the sample transfer device of the present invention, a movable wall is provided between the sample solution and the extrusion solvent, and the movable wall prevents the sample molecules in the sample solution from diffusing into the extrusion solvent.

  Specifically, it has a sample transfer channel that transfers the sample solution to the position of the detection antenna of the probe through the inner hole of the magnet device in the NMR apparatus, a movable wall in the sample transfer channel, A first stopper and a second stopper for setting the movement range are provided. An extrusion solvent for connecting a sample injection part in the vicinity of one end of the sample transfer channel, and for extruding the sample solution injected from the sample injection part into the sample transfer channel toward the detection antenna by an extrusion solvent A pump and an extrusion solvent container connected to the extrusion solvent pump are provided. An exhaust gas pump and an exhaust gas container are connected to the other end of the sample transfer channel. A sample transfer control unit that controls the sample injection unit, the extrusion solvent pump, and the exhaust gas pump is also provided. The first stopper is provided between the sample injection portion in the sample transfer channel and the extrusion solvent pump, and the second stopper is provided in the vicinity of the detection antenna.

  With the above apparatus configuration, the movable wall reciprocates between the first stopper and the second stopper due to the pressure difference between the extrusion solvent pump and the exhaust gas pump, and thereby the sample solution injected into the sample transfer channel. Is transferred to the position of the detection antenna by the sample extrusion solvent through the movable wall, and is returned from the position of the detection antenna to the sample injection portion.

  The NMR apparatus of the present invention includes the following three aspects.

  In the first aspect, the magnet device for generating a static magnetic field is provided with two room temperature bores in the vertical direction and the horizontal direction. A probe is inserted into one of the two room temperature bores, and a detection antenna is disposed at the tip of the probe near the center of the magnet device. The probe is connected to the measuring device by a high-frequency signal cable. A sample transfer channel is disposed in the longitudinal room temperature bore so as to penetrate the bore.

  A sample injection unit is connected to the vicinity of the lower end of the sample transfer channel, and further an extrusion solvent pump that extrudes the sample solution injected from the sample injection unit into the sample transfer channel toward the detection antenna by an extrusion solvent Is connected. The extrusion solvent pump is connected to the extrusion solvent container. An exhaust gas pump and an exhaust gas container are connected to the other end of the sample transfer channel. In addition, a sample transfer control unit that controls the sample injection unit, the extrusion solvent pump, and the exhaust gas pump is provided.

  A first stopper is provided between the sample injection portion and the extrusion solvent pump in the sample transfer channel, and a second stopper is provided in the vicinity of the detection antenna. A movable wall is provided between the first stopper and the second stopper in the sample transfer channel.

  Thereby, due to the pressure difference between the extrusion solvent pump and the exhaust gas pump, the movable wall provided in the sample transfer flow path reciprocates between the first stopper and the second stopper, and the sample solution passes through the movable wall. It is transferred to the detection space by the extrusion solvent and is returned from the detection space to the sample injection section.

  In the second aspect, the magnet device is provided with one longitudinal room temperature bore. A probe equipped with a detection antenna is inserted into the room temperature bore, and a sample transfer channel is provided through the room temperature bore and the probe. Other configurations are the same as those of the first aspect described above.

  In a third aspect, the magnet device is provided with at least one room temperature bore including a longitudinal room temperature bore. A probe having a detection antenna is inserted into any one of the room temperature bores, and a sample transfer channel is disposed in the room temperature bore in the vertical direction. The sample transfer channel is inserted so as to penetrate the detection antenna, and is bent into a U shape after penetrating the sample transfer channel and passes through the outside of the detection antenna to return to the original position. And a sample injection | pouring part is connected to the one edge part side of a sample transfer flow path, and also an extrusion solvent pump and an extrusion solvent container are connected. An exhaust gas pump and an exhaust gas container are connected to the other end of the sample transfer channel. Other configurations are the same as those in the first and second aspects.

  In the sample transfer apparatus and the NMR apparatus of the first to third aspects described above, the sample injection section is preferably configured to inject the sample solution into the sample transfer flow path by opening and closing a valve.

  Since the sample transfer apparatus and the NMR apparatus of the present invention use an extrusion solvent instead of gas for the transfer of the sample solution, the sample transfer time is shortened, and further, there is a movable wall between the sample solution and the extrusion solvent. There is an effect that it is possible to reduce a decrease in the concentration of the sample molecules in the detection space due to the diffusion of the sample molecules in the solution to the extrusion solvent.

  Hereinafter, the sample transfer apparatus and the NMR apparatus of the present invention will be described with reference to the drawings.

  FIG. 1 is a configuration diagram showing a first mode of an NMR apparatus including a sample transfer apparatus. In the NMR apparatus of the present embodiment, a horizontal room temperature bore 2 and a vertical room temperature bore 2 a are provided in a magnet apparatus 1 that generates a static magnetic field, and a probe 3 is inserted into the room temperature bore 2. A detection antenna 4 is disposed at the tip of the probe 3 near the center of the magnet device 1. The measuring device 5 is connected to the probe 3 by a high frequency signal cable 6. When acquiring the NMR signal, the measuring device 5 transmits a high-frequency AC magnetic field pattern to the probe 3 via the high-frequency signal cable 6 and receives a nuclear spin response signal from the probe 3 via the high-frequency signal cable 6. Data processing is performed. The processed data is provided from the user computer 7 to the user.

  The NMR apparatus of the present invention has the following configuration in addition to the configuration of the above general NMR apparatus. First, the sample transfer channel 10 is disposed through the vertical room temperature bore 2a. A sample injection unit 11 and an extrusion solvent pump 12 are connected to one end (hereinafter referred to as a first end) of the sample transfer channel 10. Further, an exhaust gas pump 13 is connected to the other end (hereinafter referred to as a second end) of the sample transfer channel 10. An extrusion solvent container 14 and an exhaust gas container 15 are connected to the extrusion solvent pump 12 and the exhaust gas pump 13, respectively. The sample injection unit 11, the extrusion solvent pump 12 and the exhaust gas pump 13 are controlled by the sample transfer control unit 16. The sample transfer control unit 16 may also serve as the measuring device 5 or the user computer 7.

  FIG. 2 is a diagram showing the sample transfer channel 10 and the sample injection unit 11 in more detail in the configuration of FIG.

  A first stopper 21 is provided between the sample injection unit 11 and the push solvent pump 12 at the first end of the sample transfer channel 10, and a second stopper 22 is provided in the sample transfer channel 10 in the vicinity of the detection antenna 4. A movable wall 20 is provided between the first end of the sample transfer channel 10 and the extrusion solvent pump 12.

  The movable wall 20 has a function of isolating the sample solution and the extrusion solvent. Due to a pressure difference between both sides of the movable wall 20, the movable wall 20 is located near the detection space from the first stopper 21 provided at the first end of the sample transfer channel 10. It reciprocates to the provided second stopper 22.

  In this embodiment, the sample injection unit 11 is connected to the first end of the sample transfer channel 10 via one or a plurality of valves 23. The sample injection unit 11 includes one or more sample containers 24, a sample preparation container 25, a sample stirrer 26, a syringe pump 27, a discharge valve 28, one or more sample discharge containers 29, a flow It consists of a path switch 30 and a filter 31.

  The sample container 24 is a syringe, and injects an internal sample into the sample preparation container 25 under the control of the user or the sample transfer control unit 16. When using a plurality of sample containers 24, each sample container includes, for example, a high-concentration sample solution containing protein molecules, an aqueous solvent with adjusted salt concentration, a buffer solution for adjusting acidity, and the protein molecules. Stores the chemical that is going to be examined for interaction. In order to further prevent the backflow of the sample solution, a valve or a check valve may be provided between the sample container 24 and the sample preparation container 25. The sample stirrer 26 is, for example, an ultrasonic sample stirrer, and stirs so that the concentration of the sample solution in the sample preparation container 25 becomes uniform. The syringe pump 27 has a function of controlling the pressure of the sample preparation container 25 to send the sample solution to the sample transfer channel 10 and a function of recovering the sample solution from the sample transfer channel 10.

  FIG. 3 is a diagram for explaining the operation performed by the sample transfer apparatus during the NMR experiment.

  FIG. 3A shows the operation during sample preparation performed before starting the NMR experiment. The sample transfer channel 10 is filled with exhaust gas injected from the exhaust gas pump 13. The movable wall 20 is placed at the position of the first stopper 21 by controlling the pressure of the extrusion solvent pump 12 and the exhaust gas pump 13. The valve 23 between the sample transfer channel 10 and the sample injection unit 11 is closed. The sample stored in the sample container 24 is injected into the sample preparation container 25 and becomes a sample solution having a uniform concentration by the sample agitator 26. The syringe pump 27 is stopped.

  FIG. 3B shows the operation of the sample transfer device when the preparation is completed and the sample solution is injected into the sample transfer channel 10. The sample agitator 26 stops operating, and the sample container 24 fixes the position of the syringe so that the sample solution does not flow backward from the sample preparation container 25. If there is a valve between the sample container 24 and the sample preparation container 25, the valve is closed. The sample solution is injected into the sample transfer channel 10 by opening the valve 23 between the sample transfer channel 10 and the sample injection unit 11 and controlling the syringe pump 27 to a positive pressure.

  FIG. 3C shows the operation of the sample transfer device when transferring the injected sample solution to the detection space. The valve 23 is closed, the pressures of the extrusion solvent pump 12 and the exhaust gas pump 13 are controlled, and the movable wall 20 is moved from the first stopper 21 to the second stopper 22. The position of the second stopper 22 is set so that the magnetization generated at the interface between the sample solution and the exhaust gas, the interface between the sample solution and the movable wall 20 and the movable wall 20 does not substantially affect the static magnetic field uniformity in the detection space. For example, if the change in the static magnetic field strength is 1 ppb or less at the center of the detection space, it can be determined that there is substantially no influence on the static magnetic field uniformity in the detection space.

  Since the sample solution and the extrusion solvent are separated by the movable wall 20, it is possible to prevent the sample molecules in the sample solution from diffusing into the extrusion solvent and reducing the concentration of the sample molecules in the detection space. Further, by stopping the movable wall 20 at the position of the second stopper 22, the sample solution can be accurately and quickly positioned in the detection space. Further, by using an extrusion solvent for transferring the sample solution and isolating the extrusion solvent and the sample solution with the movable wall 20, generation of bubbles generated by extrusion using gas and dissolution of the gas into the sample solution are prevented. it can.

  FIG. 3D shows an operation when the sample solution is collected from the detection space to the sample preparation container 25. First, the pressure of the extrusion solvent pump 12 and the exhaust gas pump 13 is controlled to move the movable wall 20 from the second stopper 22 to the first stopper 21. Next, the valve 23 is opened, the syringe pump 27 is controlled to a negative pressure, and the sample solution is collected in the sample preparation container 25.

  FIG. 3E shows an operation when the sample solution in the sample preparation container 25 is discharged. First, the valve 23 is closed and the discharge valve 28 is opened. The syringe pump 27 is controlled to a positive pressure, and the sample solution is discharged to the sample discharge container 29. The sample solution can be discharged by selecting one of the plurality of sample discharge containers 29 by the flow path and flow path switch 30. Using a plurality of sample discharge containers 29 is advantageous for analyzing and reusing discharged samples.

  Between the discharge valve 28 and the flow path switch 30, a filter 31 having selectivity with respect to the molecular size is provided. By providing the filter 31 at this position, for example, protein molecules having a large size can be left in the sample preparation container 25 and only the low-molecular chemical solution and the sample solvent can be discharged to the sample discharge container 29. If the protein molecules are left and only the low-molecular chemical solution and the sample solvent are discharged, the concentration of the protein molecules in the sample solution can be easily adjusted, and the expensive protein molecules can be easily reused. The filter 31 preferably has a replaceable structure in order to appropriately select molecules to be transmitted.

  Although not shown in the drawing, a filter may be provided between the flow path switch 30 and the sample discharge container 29. According to this configuration, a different filter can be installed for each flow path to the plurality of sample discharge containers 29. If a filter with different molecules to be permeated is provided for each flow path, the permeated molecules can be easily switched by changing the setting of the flow path switch 30.

  The sample transfer device needs to be cleaned. For this purpose, a cleaning solvent is injected into the sample container 24. When injecting the cleaning solvent into the sample container 24, only the cleaning solvent is injected into the sample preparation container 25, and after operating the sample stirrer for cleaning, injection into the sample transfer channel 10 shown in FIG. Transfer of the sample to the detection space shown in FIG. 3C, recovery from the detection space shown in FIG. 3D to the sample preparation container 25, and discharge to the sample discharge container 29 shown in FIG. Operate the device.

  The sample transfer apparatus of the present embodiment uses an extrusion solvent, so that the sample transfer time can be shortened, and the movable wall has an effect of reducing the mixing of the sample solution and the extrusion solvent and the diffusion of molecules. It is suitable for use in transferring a sample solution with high purity at high speed to an accurate position in the detection space.

  FIG. 4 is a configuration diagram showing a second mode of the NMR apparatus including the sample transfer apparatus. The magnet device 201 that creates a static magnetic field has a vertical room temperature bore 202, and a probe 203 is inserted into the room temperature bore 202. A cavity is provided in the center of the probe 203, and the sample transfer channel 10 is disposed through the cavity. Other than the above, the NMR apparatus of the first aspect is common.

  According to the above configuration, the sample transfer device of the present invention can be applied even to a magnet device having one room temperature bore.

  FIG. 5 is a diagram showing the structure of the sample transfer channel 310 of the sample transfer apparatus in the NMR apparatus of the third aspect. In the third aspect, the room temperature bore may be only one in the vertical direction, or may be two in the vertical direction and the horizontal direction. The sample transfer channel 310 is inserted into the vertical room temperature bore 302 through the detection antenna 4, bent into a U shape, passes through the outside of the detection antenna 4, and passes through the sample transfer channel 310. Comes out on the same side as the first end. Accordingly, the first end and the second end of the sample transfer channel 310 are disposed on the same side of the room temperature bore 302.

  According to the above configuration, the length of the sample transfer channel 310 is shorter than that in the first and second embodiments, so that the operability can be improved and the possibility of breakage is reduced.

It is an apparatus block diagram which shows the 1st Example of the NMR apparatus containing a sample transfer apparatus. In the first embodiment, it is a configuration diagram showing in detail a sample transfer flow path and a sample injection portion of the sample transfer device. In a 1st Example, it demonstrates the operation | movement which a sample transfer apparatus performs at the time of NMR experiment, and is a figure which shows the state at the time of sample preparation. It is a figure which shows a state when mixing is completed and a sample solution is transferred to a sample transfer channel. It is a figure which shows a state when transferring the injected sample solution to detection space. It is a figure which shows a state when collect | recovering a sample solution from a detection space to a sample preparation container. It is a figure which shows a state when discharging | emitting the sample solution in a sample preparation container. It is an apparatus block diagram which shows the 2nd Example of the NMR apparatus containing a sample transfer apparatus. It is a schematic block diagram which shows another example of a sample transfer apparatus.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Magnet apparatus, 2, 2a ... Room temperature bore, 3 ... Probe, 4 ... Detection antenna, 5 ... Measuring device, 6 ... High frequency signal cable, 7 ... User computer, 10 ... Sample transfer flow path, 11 ... Sample injection part, DESCRIPTION OF SYMBOLS 12 ... Extrusion solvent pump, 13 ... Exhaust gas pump, 14 ... Extrusion solvent container, 15 ... Exhaust gas container, 16 ... Sample transfer control part, 20 ... Movable wall, 21 ... 1st stopper, 22 ... 2nd stopper, 23 ... Valve , 24 ... Sample container, 201 ... Magnet device, 202 ... Room temperature bore, 203 ... Probe, 302 ... Room temperature bore, 310 ... Sample transfer channel.

Claims (8)

A sample transfer device for transferring a sample solution to a position where a detection antenna of a probe arranged in an inner hole of a magnet device in a nuclear magnetic resonance apparatus is provided,
Having a sample transfer channel for transferring the sample solution to the position where the detection antenna of the probe is provided through the inner hole of the magnet device
A sample injection unit on one end side of the sample transfer channel, and an extrusion solvent pump for extruding the sample solution injected from the sample injection unit into the sample transfer channel toward the detection antenna by an extrusion solvent; and Having an extrusion solvent container connected to the extrusion solvent pump;
An exhaust gas pump and an exhaust gas container on the other end side of the sample transfer channel;
A sample transfer control unit for controlling the sample injection unit, the extrusion solvent pump, and the exhaust gas pump;
A movable wall and a first stopper and a second stopper for setting a moving range of the movable wall in the sample transfer channel;
Having the first stopper between the sample injection part and the extrusion solvent pump;
Having the second stopper in the vicinity of the detection antenna;
Due to the pressure difference between the extrusion solvent pump and the exhaust gas pump, the movable wall reciprocates between the first stopper and the second stopper, so that the sample solution is moved by the sample extrusion solvent through the movable wall. A sample transfer device to a nuclear magnetic resonance apparatus characterized by being transferred.   The sample transfer device to the nuclear magnetic resonance apparatus according to claim 1, wherein the sample injection unit is configured to inject a sample solution into the sample transfer channel by opening and closing a valve. An apparatus for performing nuclear magnetic resonance measurement on a sample solution,
A magnet device for creating a static magnetic field provided with two room temperature bores in the longitudinal direction and the transverse direction;
A probe inserted into one of the two room temperature bores;
A detection antenna disposed at the tip of the probe near the center of the magnet device;
A measuring device connected to the probe by a high-frequency signal cable;
A sample transfer channel disposed through a room temperature bore in the longitudinal direction of the magnet device;
A sample injection part connected to the lower end side of the sample transfer channel;
An extrusion solvent pump for extruding the sample solution injected from the sample injection portion into the sample transfer channel toward the detection antenna by an extrusion solvent, and an extrusion solvent container connected to the extrusion solvent pump;
An exhaust gas pump and an exhaust gas container connected to the other end side of the sample transfer channel;
A sample transfer control unit for controlling the sample injection unit, the extrusion solvent pump, and the exhaust gas pump;
A first stopper provided between the sample injection part and the extrusion solvent pump in the sample transfer channel and a second stopper provided in the vicinity of the detection antenna;
A movable wall provided in the sample transfer channel and moving between the first stopper and the second stopper;
Due to the pressure difference between the extrusion solvent pump and the exhaust gas pump, the movable wall reciprocates between the first stopper and the second stopper, whereby the sample solution passes through the movable wall by the sample extrusion solvent. A nuclear magnetic resonance apparatus characterized in that it is transferred to the position of the detection antenna and returned to the sample injection section.   The nuclear magnetic resonance apparatus according to claim 3, wherein the sample injection unit is configured to inject a sample solution into the sample transfer channel by opening and closing a valve. An apparatus for performing nuclear magnetic resonance measurement on a sample solution,
A magnet device that creates a static magnetic field with a vertical room temperature bore;
A probe inserted into the room temperature bore;
A detection antenna disposed at the tip of the probe near the center of the magnet device;
A measuring device connected to the probe by a high-frequency signal cable;
A sample transfer channel disposed through the room temperature bore and the probe;
A sample injection part connected to the vicinity of the lower end of the sample transfer channel;
An extrusion solvent pump for extruding the sample solution injected from the sample injection portion into the sample transfer channel toward the detection antenna by an extrusion solvent, and an extrusion solvent container connected to the extrusion solvent pump;
An exhaust gas pump and an exhaust gas container connected to the other end of the sample transfer channel;
A sample transfer control unit for controlling the sample injection unit, the extrusion solvent pump, and the exhaust gas pump;
A first stopper provided between the sample injection portion and the extrusion solvent pump in the sample transfer channel and a second stopper provided in the vicinity of the detection antenna;
A movable wall provided in the sample transfer channel and moving between the first stopper and the second stopper;
Due to the pressure difference between the extrusion solvent pump and the exhaust gas pump, the movable wall reciprocates between the first stopper and the second stopper, whereby the sample solution passes through the movable wall by the sample extrusion solvent. A nuclear magnetic resonance apparatus characterized in that it is transferred to the position of the detection antenna and returned to the sample injection section.   The nuclear magnetic resonance apparatus according to claim 5, wherein the sample injection unit is configured to inject a sample solution into the sample transfer channel by opening and closing a valve. An apparatus for performing nuclear magnetic resonance measurement on a sample solution,
A magnet apparatus for creating a static magnetic field, provided with at least one room temperature bore including a longitudinal room temperature bore;
A probe inserted into any one of the room temperature bores;
A detection antenna disposed at the tip of the probe near the center of the magnet device;
A measuring device connected to the probe by a high-frequency signal cable;
The magnet device is inserted so as to penetrate the detection antenna through the vertical room temperature bore of the magnet device, and is arranged so as to be bent into a U shape after passing through the detection antenna and return to the original position through the outside of the detection antenna. Sample transfer flow path,
A sample injection part connected in the vicinity of one end of the sample transfer channel;
An extrusion solvent pump for extruding the sample solution injected from the sample injection portion into the sample transfer channel toward the detection antenna by an extrusion solvent, and an extrusion solvent container connected to the extrusion solvent pump;
An exhaust gas pump and an exhaust gas container connected to the other end of the sample transfer channel;
A sample transfer control unit for controlling the sample injection unit, the extrusion solvent pump, and the exhaust gas pump;
A first stopper provided between the sample injection portion of the sample transfer channel and the extrusion solvent pump and a second stopper provided in the vicinity of the detection antenna;
A movable wall provided in the sample transfer channel and moving between the first stopper and the second stopper;
Due to the pressure difference between the extrusion solvent pump and the exhaust gas pump, the movable wall reciprocates between the first stopper and the second stopper, whereby the sample solution passes through the movable wall by the sample extrusion solvent. A nuclear magnetic resonance apparatus characterized in that it is transferred to the position of the detection antenna and returned to the sample injection section.   The nuclear magnetic resonance apparatus according to claim 7, wherein the sample injection unit is configured to inject a sample solution into the sample transfer channel by opening and closing a valve.

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