JP2008519585A5 - - Google Patents

Claims (21)

(A) a polypeptide comprising an amino acid sequence having at least 60% identity with amino acids 1-42 of SEQ ID NO: 2;
Nucleotides 145 to 270 of (b) (i) SEQ ID NO: 1, and the complementary strand of the cDNA sequence contained in nucleotides from 1 to 270 (ii) SEQ ID NO: 1, or (iii) wherein (i) or (ii), 2 A polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions using SSC, washing at 60 ° C ; and (c) a conservative substitution of one or more amino acids of amino acids 1-42 of SEQ ID NO: 2 A polypeptide having antibacterial activity selected from the group consisting of variants comprising deletions and / or insertions. Polypeptide according to claim 1 in which the amino acid 1 to 42 of SEQ ID NO: 2 comprising an amino acid sequence having at least 80% identity. The polypeptide of claim 2 , comprising an amino acid sequence having at least 90% identity with amino acids 1-42 of SEQ ID NO: 2.   The polypeptide of claim 3, comprising an amino acid sequence having at least 95% identity with amino acids 1-42 of SEQ ID NO: 2. The polypeptide according to any one of claims 1 to 4 , comprising amino acids 1 to 42 of SEQ ID NO: 2. The polypeptide according to claim 5, which consists of amino acids 1 to 42 of SEQ ID NO: 2. (I) nucleotides 145 to 270 of SEQ ID NO: 1, (ii) cDNA sequence contained in nucleotides 1 to 270 of SEQ ID NO: 1, or (iii) the complementary strand of (i) or (ii) above , 2 × SSC, The polypeptide of claim 1, encoded by a polynucleotide that hybridizes under at least high stringency conditions using a wash at 65 ° C.   The polypeptide according to claim 1, wherein the polypeptide is a variant comprising a conservative substitution, deletion and / or insertion of one or more amino acids of amino acids 1 to 42 of SEQ ID NO: 2. An isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of any one of claims 1-8. 10. The isolated of claim 9 , having at least one mutation in the mature polypeptide coding sequence of SEQ ID NO: 1, wherein the variant nucleotide sequence encodes a polypeptide consisting of amino acids 1-42 of SEQ ID NO: 2. Polypeptide. 11. A nucleic acid construct comprising the polynucleotide of claim 9 or 10 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. A recombinant expression vector comprising the nucleic acid construct according to claim 11 . A recombinant host cell comprising the nucleic acid construct of claim 11 . A method for producing a polypeptide according to any one of claims 1 to 8 , wherein (a) a cell capable of producing the polypeptide in its wild type under conditions that aid in the production of the polypeptide. Or culturing the host cell of claim 13 ; and (b) recovering the polypeptide. Nucleotides 145 to 270 of (a) (i) SEQ ID NO: 1, and the complementary strand of the cDNA sequence contained in nucleotides from 1 to 270 (ii) SEQ ID NO: 1, or (iii) wherein (i) or (ii), 2 XSSC, hybridize with the DNA population under at least high stringency conditions using washing at 60 ° C ; and (b) isolate the hybridizing polynucleotide encoding a polypeptide having antibacterial activity An isolated polynucleotide obtained by: (A) culturing a transgenic plant or plant cell comprising a polynucleotide encoding a polypeptide having antibacterial activity of the present invention under conditions that aid in the production of said polypeptide; and (b) The method for producing a polypeptide according to any one of claims 1 to 8 , comprising recovering the polypeptide. A transgenic plant, plant part, or plant cell that has been transformed with a polynucleotide encoding the polypeptide of any one of claims 1-8 . A method for killing or inhibiting the growth of a microbial cell comprising contacting the microbial cell with an antimicrobial polypeptide according to any one of claims 1-8 . The antibacterial polypeptide according to any one of claims 1 to 8 , for use as a medicine, or a veterinary or human antibacterial therapeutic or prophylactic agent. Use of an antimicrobial polypeptide according to any one of claims 1 to 8 in the preparation of a veterinary or human therapeutic agent for the treatment or prevention use of microbial infections. Use of at least one antimicrobial polypeptide according to any one of claims 1 to 8 in animal feed.