JP2008275592A5

JP2008275592A5 -

Info

Publication number: JP2008275592A5
Application number: JP2008051309A
Authority: JP
Filing date: 2008-02-29
Publication date: 2011-04-07
Anticipated expiration: 2028-02-29

Claims

An alkaline phosphatase (ALP) labeling substance capable of binding to the target substance;
A substrate solution containing a substrate for ALP;
An auxiliary reagent solution that is added when the ALP of the ALP-labeled substance bound to the target substance reacts with the substrate, containing an inhibitor for inhibiting endogenous ALP;
A reagent kit for detecting ALP label.

2. The reagent kit for detecting an ALP label according to claim 1, wherein the target substance is a measurement target substance in a complex including a measurement target substance and a first binding substance that can bind to the target substance by an antigen-antibody reaction.

Used for immunoassay by competitive method,
The reagent kit for detecting an ALP label according to claim 1, wherein the target substance is a first binding substance that can be bound to a measurement target substance by an antigen-antibody reaction.

The reagent kit for detecting an ALP label according to any one of claims 1 to 3, wherein the ALP labeling substance includes a second binding substance capable of binding to a target substance and ALP.

The reagent kit for detecting an ALP label according to any one of claims 1 to 4, wherein the mixture of the ALP labeling substance, the substrate solution and the auxiliary reagent solution is alkaline.

The reagent kit for detecting an ALP label according to any one of claims 1 to 5, wherein the substrate solution is alkaline.

The ALP label detection reagent kit according to any one of claims 1 to 6, wherein the auxiliary reagent solution is neutral or acidic.

The substrate solution and the auxiliary reagent solution contain a buffer,
The reagent kit for detecting an ALP label according to any one of claims 1 to 7, wherein the substrate solution has a buffer capacity stronger than that of the auxiliary reagent solution.

The reagent kit for detecting ALP label according to any one of claims 1 to 8, wherein the inhibitor is levamisole.

The ALP label detection reagent kit according to any one of claims 1 to 9, wherein the ALP is a bacterium-derived ALP or an intestine-derived ALP.

A first binding substance that can be bound to an analyte by an antigen-antibody reaction;
A solid phase for supporting a complex containing a substance to be measured and a first binding substance;
An alkaline phosphatase (ALP) labeled substance capable of binding to the substance to be measured in the complex;
A substrate solution containing a substrate for ALP;
An auxiliary reagent solution that is added when reacting the ALP of the ALP-labeled substance bound to the measurement target substance in the complex with the substrate, which contains an inhibitor for inhibiting endogenous ALP;
A reagent kit for immunoassay comprising:

An immunoassay reagent kit used for immunoassay by a competitive method,
An alkaline phosphatase (ALP) labeling substance;
A first binding substance capable of binding to the substance to be measured and the ALP-labeled substance by an antigen-antibody reaction;
A solid phase for supporting the first binding substance;
A substrate solution containing a substrate for ALP;
An auxiliary reagent solution that is added when reacting the ALP of the ALP-labeled substance bound to the first binding substance and the substrate, containing an inhibitor for inhibiting endogenous ALP;
A reagent kit for immunoassay comprising:

A substance to be measured in the sample, an alkaline phosphatase (ALP) labeled substance capable of binding to the substance to be measured, a first binding substance capable of binding to the substance to be measured by an antigen-antibody reaction and capable of binding to a solid phase Forming a complex on the solid phase,
The ALP of the ALP-labeled substance bound to the substance to be measured in the complex and the substrate for ALP contained in the substrate solution are reacted in the presence of an auxiliary reagent solution containing an inhibitor for inhibiting endogenous ALP. And detecting a reaction product produced by the reaction between ALP and the substrate,
An immunoassay method comprising:

A competitive immunoassay method,
A specimen, an alkaline phosphatase (ALP) -labeled substance, a first binding substance capable of binding to the ALP-labeled substance and the measurement target substance in the specimen by an antigen-antibody reaction, and a solid phase for supporting the first binding substance Mixing,
In the presence of an auxiliary reagent solution containing an ALP labeled substance ALP bound to the first binding substance supported on the solid phase and a substrate for ALP contained in the substrate solution, which contains an inhibitor for inhibiting endogenous ALP. The step of reacting with ALP, and the step of detecting the reaction product produced by the reaction of ALP with the substrate,
An immunoassay method comprising:

The immunoassay method according to claim 14, wherein the mixing step is performed by mixing the specimen, the first binding substance, and the solid phase, and then mixing the ALP-labeled substance in the mixture.

The immunoassay method according to claim 14, wherein the mixing step is performed by mixing a sample, a first binding substance, and an ALP-labeled substance, and then mixing a solid phase with the mixture.