JP2008275592A5 - - Google Patents
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- JP2008275592A5 JP2008275592A5 JP2008051309A JP2008051309A JP2008275592A5 JP 2008275592 A5 JP2008275592 A5 JP 2008275592A5 JP 2008051309 A JP2008051309 A JP 2008051309A JP 2008051309 A JP2008051309 A JP 2008051309A JP 2008275592 A5 JP2008275592 A5 JP 2008275592A5
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- JP
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- Prior art keywords
- alp
- substance
- binding
- substrate
- reagent kit
- Prior art date
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- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 53
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 53
- 239000000126 substance Substances 0.000 claims 48
- 239000003153 chemical reaction reagent Substances 0.000 claims 22
- 239000000758 substrate Substances 0.000 claims 19
- 230000002401 inhibitory effect Effects 0.000 claims 11
- 238000003018 immunoassay Methods 0.000 claims 9
- 239000007790 solid phase Substances 0.000 claims 8
- 239000003112 inhibitor Substances 0.000 claims 6
- 238000005259 measurement Methods 0.000 claims 5
- 238000002372 labelling Methods 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 3
- 239000007795 chemical reaction product Substances 0.000 claims 2
- 230000002860 competitive Effects 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 210000000936 Intestines Anatomy 0.000 claims 1
- HLFSDGLLUJUHTE-SNVBAGLBSA-N Levotetramisole Chemical group C1([C@H]2CN3CCSC3=N2)=CC=CC=C1 HLFSDGLLUJUHTE-SNVBAGLBSA-N 0.000 claims 1
- 230000002378 acidificating Effects 0.000 claims 1
- 239000012491 analyte Substances 0.000 claims 1
- 238000002967 competitive immunoassay Methods 0.000 claims 1
- 229960001614 levamisole Drugs 0.000 claims 1
- 230000001264 neutralization Effects 0.000 claims 1
Claims (16)
ALPに対する基質を含む基質液と、
内因性ALPを阻害するための阻害剤を含み、前記目的物質に結合したALP標識化物質のALPと基質とを反応させる際に添加される補助試薬液と、
を含むALP標識検出用試薬キット。 An alkaline phosphatase (ALP) labeling substance capable of binding to the target substance;
A substrate solution containing a substrate for ALP;
An auxiliary reagent solution that is added when the ALP of the ALP-labeled substance bound to the target substance reacts with the substrate, containing an inhibitor for inhibiting endogenous ALP;
A reagent kit for detecting ALP label.
前記目的物質は、測定対象物質に抗原抗体反応により結合可能な第1結合物質である請求項1に記載のALP標識検出用試薬キット。 Used for immunoassay by competitive method,
The reagent kit for detecting an ALP label according to claim 1, wherein the target substance is a first binding substance that can be bound to a measurement target substance by an antigen-antibody reaction.
前記基質液が、前記補助試薬液よりも強い緩衝能を有する請求項1〜7の何れか1項に記載のALP標識検出用試薬キット。 The substrate solution and the auxiliary reagent solution contain a buffer,
The reagent kit for detecting an ALP label according to any one of claims 1 to 7, wherein the substrate solution has a buffer capacity stronger than that of the auxiliary reagent solution.
測定対象物質及び第1結合物質を含む複合体を担持するための固相と、
複合体中の測定対象物質と結合可能なアルカリホスファターゼ(ALP)標識化物質と、
ALPに対する基質を含む基質液と、
内因性ALPを阻害するための阻害剤を含み、複合体中の測定対象物質に結合したALP標識化物質のALPと基質とを反応させる際に添加される補助試薬液と、
を含む免疫測定用試薬キット。 A first binding substance that can be bound to an analyte by an antigen-antibody reaction;
A solid phase for supporting a complex containing a substance to be measured and a first binding substance;
An alkaline phosphatase (ALP) labeled substance capable of binding to the substance to be measured in the complex;
A substrate solution containing a substrate for ALP;
An auxiliary reagent solution that is added when reacting the ALP of the ALP-labeled substance bound to the measurement target substance in the complex with the substrate, which contains an inhibitor for inhibiting endogenous ALP;
A reagent kit for immunoassay comprising:
アルカリホスファターゼ(ALP)標識化物質と、
測定対象物質及びALP標識化物質に抗原抗体反応により結合可能な第1結合物質と、
第1結合物質を担持するための固相と、
ALPに対する基質を含む基質液と、
内因性ALPを阻害するための阻害剤を含み、第1結合物質に結合したALP標識化物質のALPと基質とを反応させる際に添加される補助試薬液と、
を含む免疫測定用試薬キット。 An immunoassay reagent kit used for immunoassay by a competitive method,
An alkaline phosphatase (ALP) labeling substance;
A first binding substance capable of binding to the substance to be measured and the ALP-labeled substance by an antigen-antibody reaction;
A solid phase for supporting the first binding substance;
A substrate solution containing a substrate for ALP;
An auxiliary reagent solution that is added when reacting the ALP of the ALP-labeled substance bound to the first binding substance and the substrate, containing an inhibitor for inhibiting endogenous ALP;
A reagent kit for immunoassay comprising:
前記複合体中の測定対象物質と結合したALP標識化物質のALPと、基質液に含まれるALPに対する基質とを、内因性ALPを阻害するための阻害剤を含む補助試薬液の存在下で反応させる工程、及び
ALPと基質の反応により産生された反応産物を検出する工程、
からなる免疫測定方法。 A substance to be measured in the sample, an alkaline phosphatase (ALP) labeled substance capable of binding to the substance to be measured, a first binding substance capable of binding to the substance to be measured by an antigen-antibody reaction and capable of binding to a solid phase Forming a complex on the solid phase,
The ALP of the ALP-labeled substance bound to the substance to be measured in the complex and the substrate for ALP contained in the substrate solution are reacted in the presence of an auxiliary reagent solution containing an inhibitor for inhibiting endogenous ALP. And detecting a reaction product produced by the reaction between ALP and the substrate,
An immunoassay method comprising:
検体と、アルカリホスファターゼ(ALP)標識化物質と、ALP標識化物質及び検体中の測定対象物質に抗原抗体反応により結合可能な第1結合物質と、第1結合物質を担持するための固相とを混合させる工程、
固相に担持された第1結合物質に結合したALP標識化物質のALPと、基質液に含まれるALPに対する基質とを、内因性ALPを阻害するための阻害剤を含む補助試薬液の存在下で反応させる工程、及び
ALPと基質の反応により産生された反応産物を検出する工程、
からなる免疫測定方法。 A competitive immunoassay method,
A specimen, an alkaline phosphatase (ALP) -labeled substance, a first binding substance capable of binding to the ALP-labeled substance and the measurement target substance in the specimen by an antigen-antibody reaction, and a solid phase for supporting the first binding substance Mixing,
In the presence of an auxiliary reagent solution containing an ALP labeled substance ALP bound to the first binding substance supported on the solid phase and a substrate for ALP contained in the substrate solution, which contains an inhibitor for inhibiting endogenous ALP. The step of reacting with ALP, and the step of detecting the reaction product produced by the reaction of ALP with the substrate,
An immunoassay method comprising:
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008051309A JP5137620B2 (en) | 2007-04-03 | 2008-02-29 | Reagent kit for detecting alkaline phosphatase label, reagent kit for immunoassay, and immunoassay method |
CN 200810090640 CN101281200B (en) | 2007-04-03 | 2008-04-02 | Immunoassay method, reagent kit for detecting alkaline phosphatase, and reagent kit for immunoassay |
AT08006730T ATE458999T1 (en) | 2007-04-03 | 2008-04-02 | IMMUNASSAY METHOD, REAGENT KIT FOR DETECTING AN ALKALINE PHOSPHATASE AND REAGENT KIT FOR THE IMMUNASSAY METHOD |
DE200860000683 DE602008000683D1 (en) | 2007-04-03 | 2008-04-02 | Immunoassay method, reagent kit for detection of an alkaline phosphatase and reagent kit for the immunoassay method |
EP20080006730 EP1978363B1 (en) | 2007-04-03 | 2008-04-02 | Immunoassay method, reagent kit for detecting alkaline phosphatase, and reagent kit for immunoassay |
US12/062,170 US9046516B2 (en) | 2007-04-03 | 2008-04-03 | Immunoassay method, reagent kit for detecting alkaline phosphatase, and reagent kit for immunoassay |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007096980 | 2007-04-03 | ||
JP2007096980 | 2007-04-03 | ||
JP2008051309A JP5137620B2 (en) | 2007-04-03 | 2008-02-29 | Reagent kit for detecting alkaline phosphatase label, reagent kit for immunoassay, and immunoassay method |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2008275592A JP2008275592A (en) | 2008-11-13 |
JP2008275592A5 true JP2008275592A5 (en) | 2011-04-07 |
JP5137620B2 JP5137620B2 (en) | 2013-02-06 |
Family
ID=40013764
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2008051309A Active JP5137620B2 (en) | 2007-04-03 | 2008-02-29 | Reagent kit for detecting alkaline phosphatase label, reagent kit for immunoassay, and immunoassay method |
Country Status (4)
Country | Link |
---|---|
JP (1) | JP5137620B2 (en) |
CN (1) | CN101281200B (en) |
AT (1) | ATE458999T1 (en) |
DE (1) | DE602008000683D1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011102065A1 (en) * | 2010-02-17 | 2011-08-25 | 株式会社 村田製作所 | Method of detection using mass detection sensor |
CN102297962B (en) * | 2011-05-23 | 2014-01-08 | 董理 | Kit for detecting alkaline phosphatase |
CN102706869B (en) * | 2012-06-05 | 2014-09-17 | 宁波美康生物科技股份有限公司 | System for amplifying alkaline phosphatase label signal |
EP3279661B1 (en) * | 2015-03-31 | 2020-09-09 | Sysmex Corporation | Immunoassay device |
US11047854B2 (en) | 2017-02-06 | 2021-06-29 | Abbott Japan Llc | Methods for reducing noise in signal-generating digital assays |
WO2019111355A1 (en) * | 2017-12-06 | 2019-06-13 | オリンパス株式会社 | Learning model creation method, image processing method, learning model creation device, image processing device, learning model creation program, and image processing program |
CN113267390B (en) * | 2021-05-11 | 2023-05-05 | 北京丹大生物技术有限公司 | Detection marker eluent for filter paper dried blood slices and application thereof |
CN117554629B (en) * | 2024-01-11 | 2024-05-10 | 中国医学科学院北京协和医院 | Marker for differential diagnosis of neurosyphilis and application thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5135847A (en) * | 1988-11-18 | 1992-08-04 | Becton, Dickinson And Company | Substrate composition for alkaline phosphatase and method for assay using same |
JP3350730B2 (en) * | 1993-02-17 | 2002-11-25 | デイド、ベーリング、インコーポレイテッド | Methods and compositions for reducing the effects of endogenous alkaline phosphatase |
JP2001183374A (en) * | 1999-12-24 | 2001-07-06 | Tosoh Corp | Conjugate preservation liquid |
-
2008
- 2008-02-29 JP JP2008051309A patent/JP5137620B2/en active Active
- 2008-04-02 AT AT08006730T patent/ATE458999T1/en not_active IP Right Cessation
- 2008-04-02 CN CN 200810090640 patent/CN101281200B/en active Active
- 2008-04-02 DE DE200860000683 patent/DE602008000683D1/en active Active
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