JP2008161075A - Method for rearing immature of macrocheira kaempferi, and immature of macrocheira kaempferi reared by the method - Google Patents

Method for rearing immature of macrocheira kaempferi, and immature of macrocheira kaempferi reared by the method Download PDF

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JP2008161075A
JP2008161075A JP2006351298A JP2006351298A JP2008161075A JP 2008161075 A JP2008161075 A JP 2008161075A JP 2006351298 A JP2006351298 A JP 2006351298A JP 2006351298 A JP2006351298 A JP 2006351298A JP 2008161075 A JP2008161075 A JP 2008161075A
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juvenile
crab
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Kazutoshi Okamoto
一利 岡本
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Shizuoka Prefecture
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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    • Y02A40/81Aquaculture, e.g. of fish

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for rearing immatures of Macrocheira kaempferi, which improves a survival rate at an initial growth stage of Macrocheira kaempferi, and to obtain immatures of Macrocheira kaempferi reared by the method. <P>SOLUTION: The method for rearing immatures of Macrocheira kaempferi comprises rearing Macrocheira kaempferi, at an initial growth stage in rearing water in the presence of an aminoglycoside-based antibiotic and a chloramphenicol-based antibiotic. In the rearing method, the aminoglycoside-based antibiotic preferably exists in the range of 0.04-0.07 g, and the chloramphenicol-based antibiotic, preferably, exists in the range of 0.01-0.03 g based on 1 liter of rearing water, respectively. The immatures of Macrocheira kaempferi are reared by the rearing method. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

本発明は、タカアシガニの初期成長段階の生残率を向上させるタカアシガニ幼体の飼育方法及びその方法により飼育されたタカアシガニ幼体に関するものである。   The present invention relates to a method for breeding a hawk crab juvenile that improves the survival rate in the initial growth stage of the hawk crab, and a hawk crab juvenile bred by the method.

タカアシガニは、両脚を伸ばすと3mを越す世界最大のカニである。タカアシガニは、日本国内のみならず世界各国の水族館等で飼育・展示が行われ、世界から注目されている人気の高いカニである。タカアシガニの生息分布は日本の太平洋岸で、その生息数はさほど多くはない。タカアシガニの水産利用のみならず展示利用にあたって、あまり知られていないタカアシガニの生態の解明や、天然資源への配慮のためにも、タカアシガニの繁殖技術は不可欠である。   Takaashigani is the world's largest crab that exceeds 3m when both legs are extended. Takashi Crab is a popular crab that has been bred and exhibited not only in Japan but also in aquariums around the world. Takashi crabs live in the Pacific coast of Japan, and the number of them is not very high. In order to elucidate the ecology of hawk crabs, which are not well known, and to consider natural resources, not only the use of hawk crabs but also marine products, the breeding technology of hawk crabs is indispensable.

タカアシガニの成長過程として、卵(約0.7mm)からふ化したゾエア第1期幼生(約1.5mm)は、ゾエア第2期(約1.6mm)、メガロパ期(約1.8mm)を経て稚ガニ1齢期(約2mm)に脱皮成長し、その後も、稚ガニ2齢期(約3mm)、3齢期(約4mm)・・・と脱皮成長することが知られている。 As a growth process of hawk crabs, the first stage larvae (about 1.5 mm) hatched from eggs (about 0.7 mm) passed through the second stage (about 1.6 mm) and the megalopa stage (about 1.8 mm). It is known that molting occurs at the first infancy of the juvenile crab (about 2 mm), and after that, molting and growth of the juvenile crab at the second infancy (about 3 mm), the third infancy (about 4 mm), and so on.

タカアシガニの幼生飼育に関しては多くの事例があり、飼育水にペニシリンやストレプトマイシン等の抗生物質を使用することにより、稚ガニ1齢期までの生残率が高まることが知られている。抗生物質を使用することにより甲殻類の生残率が高まることは、例えばクルマエビにおいても知られている(特開昭57-75924号公報)。 There are many cases of raising larvae of hawk crabs, and it is known that the survival rate up to the first infancy of juvenile crabs increases by using antibiotics such as penicillin and streptomycin in the breeding water. The increase in the survival rate of crustaceans by using antibiotics is also known, for example, in prawns (Japanese Patent Laid-Open No. 57-75924).

しかし、ペニシリンやストレプトマイシンを使用した過去のタカアシガニの飼育事例においては、ある成長段階において極端に生残率が低く、大きな問題となっていた。 However, in the past hawk crab breeding cases using penicillin or streptomycin, the survival rate was extremely low at a certain growth stage, which was a big problem.

過去の飼育事例における各発育段階の平均生残率は以下のとおりである。
ふ化したゾエア第1期からゾエア第2期までは63.8%、ゾエア第2期からメガロパ期は52.5%、メガロパ期から稚ガニ1齢期は6.8%、稚ガニ1齢期から2齢期は15.6%、2齢期から3齢期は52.8%、3齢期から4齢期は66%、4齢期から5齢期は80.3%であった。
The average survival rate at each developmental stage in past breeding cases is as follows.
From the first stage of hatched Zoea to the second stage of Zoea, 63.8%, from the second stage of Zoea to 52.5% of the megalopa period, 6.8% from the first stage of the megalopa period to the juvenile crab, and the first juvenile period From 2 to 3 years, 15.6%, from 2 to 3 years were 52.8%, from 3 to 4 years were 66%, from 4 to 5 years were 80.3%.

メガロパ期から稚ガニ1齢期と、稚ガニ1齢期から2齢期までの生残率が極端に低く、メガロパ期から稚ガニ2齢期までの生残率が1%、ゾエア第1期から稚ガニ2齢期まで通した生残率が0.4%であった。
特開昭57-75924号公報
Survival rate from megalopa period to juvenile crab 1st stage and juvenile crab 1st stage to 2nd stage is extremely low, survival rate from megalopa period to juvenile crab 2nd stage is 1%, zoea first stage Survival rate passed through from juvenile crab to the second infancy was 0.4%.
JP 57-75924 A

タカアシガニを飼育する上で、その初期成長段階、特にメガロパ期幼生から稚ガニ2齢期までの低い生残率を向上させることが大きな課題である。   In rearing hawk crabs, it is a major challenge to improve the initial survival stage, particularly the low survival rate from the megalopa stage larvae to the juvenile crab 2 stage.

本発明は、上記課題を解決するために、タカアシガニの初期成長段階の生残率を向上させる飼育方法を提供することを目的とする。 In order to solve the above-mentioned problems, an object of the present invention is to provide a breeding method that improves the survival rate of the initial growth stage of the king crab.

上記課題を解決するために、本発明は、下記の如きタカアシガニ幼体の飼育方法及びその方法により飼育されたタカアシガニ幼体を提供する。 In order to solve the above-mentioned problems, the present invention provides a method for rearing a Takashi Crab juvenile as described below and a Takashi Crab juvenile reared by the method.

(1)アミノグリコシド系抗生物質とクロラムフェニコール系抗生物質とが存在する飼育水中で、初期成長段階のタカアシガニを飼育することを特徴とするタカアシガニ幼体の飼育方法(請求項1)。 (1) A method for rearing a hawk crabs juvenile characterized by cultivating a crabs in an initial growth stage in a breeding water in which an aminoglycoside antibiotic and a chloramphenicol antibiotic are present (claim 1).

(2)前記飼育方法において、飼育水1リットルあたり、前記アミノグリコシド系抗生物質が0.04〜0.07gの範囲内で、前記クロラムフェニコール系抗生物質が0.01〜0.03gの範囲内で、それぞれ存在することが望ましい(請求項2)。 (2) In the breeding method, the aminoglycoside antibiotic is in the range of 0.04 to 0.07 g and the chloramphenicol antibiotic is in the range of 0.01 to 0.03 g per liter of the breeding water. It is desirable that each of them exists within the range (claim 2).

(3)前記アミノグリコシド系抗生物質はストレプトマイシンである(請求項3)。 (3) The aminoglycoside antibiotic is streptomycin (claim 3).

(4)前記初期成長段階は、下記(a)〜(f)のいずれかである(請求項4)。
(a)ゾエア期
(b)メガロパ期
(c)稚ガニ期
(d)ゾエア期とメガロパ期
(e)メガロパ期と稚ガニ期
(f)ゾエア期とメガロパ期と稚ガニ期
(4) The initial growth stage is any one of the following (a) to (f).
(A) Zoea period (b) Megalopa period (c) Juvenile crab period (d) Zoea period and Megalopa period (e) Megalopa period and juvenile crab period (f) Zoea period, Megalopa period and juvenile crab period

(5)前記飼育水は海洋深層水である(請求項5)。 (5) The breeding water is deep ocean water (Claim 5).

(6)前記(1)から(5)のいずれかの飼育方法により飼育されたタカアシガニ幼体(請求項6)。 (6) A hawk crab juvenile bred by the breeding method according to any one of (1) to (5) (Claim 6).

本発明において、「海洋深層水」とは、海洋の水深100m以深から汲み上げた海水をいう。なお、「表層海水」とは、海洋の水深100m未満から汲み上げた海水をいう。   In the present invention, “deep ocean water” refers to seawater pumped from a depth of 100 m or more in the ocean. “Surface seawater” refers to seawater drawn from the depth of the ocean below 100 m.

本発明は、下記の如き優れた効果を発揮する。   The present invention exhibits the following excellent effects.

(イ)本発明により、タカアシガニの初期成長段階の生残率を向上させることが可能となり、本発明の飼育方法をタカアシガニの繁殖技術として利用することができる。 (I) According to the present invention, it is possible to improve the survival rate in the initial growth stage of hawk crab, and the breeding method of the present invention can be used as a technique for breeding hawk crabs.

(ロ)本発明は、タカアシガニ幼体を効率よく飼育することを可能ならしめ、その生態解明、展示、増養殖、種の保存に貢献する実用性に優れた効果を有するものである。 (B) The present invention makes it possible to efficiently rear hawk crabs, and has an excellent utility in contributing to elucidation of ecology, display, aquaculture, and species preservation.

(ハ)更に、本発明は、飼育されたタカアシガニを国内外の研究機関や水族館等へ提供することも可能ならしめるものであり、その実用的効果は大きい。 (C) Furthermore, the present invention makes it possible to provide domestic hawk crabs to domestic and foreign research institutions, aquariums, etc., and its practical effect is great.

本発明によるタカアシガニ幼体の飼育方法においては、アミノグリコシド系抗生物質とクロラムフェニコール系抗生物質とが存在する飼育水中で、初期成長段階のタカアシガニを飼育する。   In the method for rearing a hawk crab juvenile according to the present invention, a hawk crab in an initial growth stage is bred in a breeding water in which an aminoglycoside antibiotic and a chloramphenicol antibiotic are present.

タカアシガニ(Macrocheira Kaempferi)は、世界最大のカニであって、世界から注目されている人気の高いカニである。 Takashi Crab (Macrocheira Kaempferi) is the world's largest crab and is a popular crab that is attracting attention from the world.

幼体とは、ふ化後から稚ガニまでの初期成長段階のことである。 The juvenile is the initial stage of growth from hatching to juvenile crabs.

アミノグリコシド系抗生物質とは、カナマイシン、ストレプトマイシン、ネオマイシン、ゲンタマイシン、フラジオマイシン、トブラマイシン、アミカシン、アルベカシン、アストロマイシン、イセパマイシン、ベカナマイシン、ジベカシン、ミクロノマイシン、ネチルマイシン、バロモマイシン、リボスタマイシン、シソマイシン等のことである。 Aminoglycoside antibiotics include kanamycin, streptomycin, neomycin, gentamicin, fradiomycin, tobramycin, amikacin, arbekacin, astromycin, isepamicin, bekanamycin, dibekacin, micronomycin, netilmycin, valomomycin, ribostamycin, sisomycin, etc. It is.

クロラムフェニコール系抗生物質とは、クロラムフェニコール等のことである。 The chloramphenicol antibiotic is chloramphenicol or the like.

飼育水1リットルあたり、アミノグリコシド系抗生物質が0.04〜0.07gの範囲内で、クロラムフェニコール系抗生物質が0.01〜0.03gの範囲内で、それぞれ存在することが好ましい。けだし、抗生物質の割合が当該範囲を下回るときには当該抗生物質はその効能を発揮せず、抗生物質の割合が当該範囲を上回るときには当該抗生物質はタカアシガニ幼体の生残に悪影響をおよぼすからである。 Preferably, the aminoglycoside antibiotic is present in the range of 0.04 to 0.07 g and the chloramphenicol antibiotic is present in the range of 0.01 to 0.03 g per liter of the breeding water. However, when the proportion of the antibiotic is below the range, the antibiotic does not exert its effect, and when the proportion of the antibiotic exceeds the range, the antibiotic has an adverse effect on the survival of the king crab juvenile.

アミノグリコシド系抗生物質はストレプトマイシンであることが好ましい。けだし、ストレプトマイシンを使用することにより、タカアシガニ幼体の生残が良好になるからである。 The aminoglycoside antibiotic is preferably streptomycin. However, the use of streptomycin improves the survival of the king crab larvae.

対象となるタカアシガニの成長段階は、初期成長段階の一部もしくはすべての成長段階、すなわち、下記(a)〜(f)のいずれかである。
(a)ゾエア期
(b)メガロパ期
(c)稚ガニ期
(d)ゾエア期とメガロパ期
(e)メガロパ期と稚ガニ期
(f)ゾエア期とメガロパ期と稚ガニ期
The growth stage of the target crabs is one or all of the initial growth stages, that is, any of the following (a) to (f).
(A) Zoea period (b) Megalopa period (c) Juvenile crab period (d) Zoea period and Megalopa period (e) Megalopa period and juvenile crab period (f) Zoea period, Megalopa period and juvenile crab period

飼育水は、好ましくは、海洋深層水である。けだし、飼育水として海洋深層水を使用することにより、タカアシガニ幼体の生存が良好になるからである。 The breeding water is preferably deep ocean water. However, the use of deep ocean water as breeding water improves the survival of juvenile hawk crabs.

本発明の飼育方法により飼育されたタカアシガニ幼体は、生態解明、展示、増養殖、種の保存等として利用可能である。 The king crab juvenile bred by the breeding method of the present invention can be used for ecological elucidation, display, aquaculture, species preservation, and the like.

以下、本発明の実施例について説明するが、本発明はこれらの各実施に限定されるものではない。 Examples of the present invention will be described below, but the present invention is not limited to these examples.

メガロパ期から稚ガニ2齢期までの飼育 Breeding from Megalopa period to juvenile crab 2

<材料と方法>
抱卵親からふ化したゾエア第1期幼生を飼育することにより得たメガロパ期幼生を実験に供試した。
<Materials and methods>
Megalopa stage larvae obtained by rearing zoea stage 1 larvae hatched from the laying parent were used for experiments.

下記の合計8実験区を設定した。
・飼育水1リットルあたりペニシリンGカリウム(C1617KNS)を0.1g添加したP区。
・飼育水1リットルあたりストレプトマイシン硫酸塩(C213912・1.5HSO4)を0.05g添加したS区。
・飼育水1リットルあたりクロラムフェニコール(C1112Cl)を0.025g添加したC区。
・上記3種の抗生物質のうち2種を添加したPS区と、PC区と、SC区。
・上記3種の抗生物質をすべて添加したPSC区。
・上記3種の抗生物質のいずれをも添加していないN区。
The following 8 experimental zones were set in total.
-P section to which 0.1 g of penicillin G potassium (C 16 H 17 KN 2 O 4 S) was added per liter of breeding water.
· S Ward added 0.05g breeding water per liter streptomycin sulfate (C 21 H 39 N 7 O 12 · 1.5H 2 SO 4).
· Breeding water per liter of chloramphenicol (C 11 H 12 Cl 2 N 2 O 5) C Ward added 0.025g of.
-PS, PC, and SC with the addition of two of the above three antibiotics.
-PSC section to which all three antibiotics are added.
-N section to which none of the above three antibiotics is added.

1実験区につき2リットルフィンガーボール1容器に17〜23個体ずつメガロパ期幼生を収容した。 17-23 individuals of megalopa stage larvae were housed in one 2 liter fingerball container per experimental group.

飼育水は、ウォーターバスにより水温を平均15.3℃に調節し、緩やかに通気した。 The breeding water was aerated gently by adjusting the water temperature to an average of 15.3 ° C. with a water bath.

飼育水の交換は、1日おきに幼生を事前に用意した飼育容器に移し替えることにより行った。 The breeding water was exchanged by transferring larvae to breeding containers prepared in advance every other day.

餌料としてアルテミアノープリウスを5個体/mlになるように投与し、更に冷凍サクラエビの細片を併用した。 Artemia naprius was administered as a feed at 5 individuals / ml, and frozen cherry shrimp strips were used in combination.

メガロパ期から稚ガニ2齢期まで飼育した。 It was raised from the megalopa period to the juvenile crab 2nd year.

<結果>
S区とSC区ともに実験開始46〜56日後に稚ガニ2齢期に成長した。それらの生残率は、S区で4.5%、SC区で34.8%であった。これら以外の区は稚ガニ2齢期まで成長しなかった。SC区において、メガロパ期から稚ガニ2齢期までの生残率が極端に高かった(χ検定, P<0.01)。従来の飼育方法ではその生残率は1%であるので、大幅な生残率の向上が認められた。
<Result>
Both the S and SC wards grew to juvenile crab 2 years after the start of the experiment. Their survival rate was 4.5% in the S district and 34.8% in the SC district. Other wards did not grow until the juvenile crab 2nd year. In the SC ward, the survival rate from the megalopa stage to the juvenile crab 2nd stage was extremely high (χ 2 test, P <0.01). In the conventional breeding method, the survival rate was 1%, so a significant improvement in the survival rate was recognized.

ゾエア第1期から稚ガニ2齢期までの飼育 Breeding from the first stage of zoea to the second stage of juvenile crab

<材料と方法>
抱卵親からふ化した240個体のゾエア第1期幼生を実験に供試した。
<Materials and methods>
240 zoea first stage larvae hatched from laying parents were used for the experiment.

下記の4実験区を設定した。
・SC区の飼育水として水深687mから取水した海洋深層水を用いてなるSC区の海洋深層水区。
・SC区の飼育水として水深24mから取水した表層水を用いてなるSC区の表層水区。
・N区の飼育水として水深687mから取水した海洋深層水を用いてなるN区の海洋深層水区。
・N区の飼育水として水深24mから取水した表層水を用いてなるN区の表層水区。
The following 4 experimental zones were set up.
-Deep sea water section of SC area using deep sea water taken from a depth of 687m as breeding water for SC area.
・ Surface water area of SC area using surface water taken from 24m depth as breeding water of SC area.
・ N-district deep-sea water zone using deep-sea water taken from 687m in depth as breeding water of N-ku.
-Surface water area of N ward using surface water taken from a depth of 24m as breeding water of N ward.

なお、SC区は、上記実施例1におけるSC区と同様に、飼育水1リットルあたりストレプトマイシン硫酸塩を0.05gとクロラムフェニコールを0.025gとを添加したものであり、N区は、上記実施例1におけるN区と同様に、抗生物質を全く添加していないものである。 In addition, like the SC group in Example 1 above, the SC group was prepared by adding 0.05 g of streptomycin sulfate and 0.025 g of chloramphenicol per liter of breeding water. Similar to the N section in Example 1 above, no antibiotics were added.

1実験区3個の1リットル容ビーカーを使用し、1ビーカーに20個体ずつ幼生を収容し、各実験区とも各々60個体飼育した。 Three 1-liter beakers were used in one experimental group, 20 larvae were housed in one beaker, and 60 individuals were raised in each experimental group.

飼育水は、ウォーターバスにより水温をゾエア期間では平均16.9℃、メガロパ期間では平均14.5℃に調節し、緩やかに通気した。 The breeding water was gently aerated by adjusting the water temperature to an average of 16.9 ° C. during the zoea period and an average of 14.5 ° C. during the megalopa period using a water bath.

飼育水の交換は、1日おきに幼生を事前に用意した飼育容器に移し替えることにより行った。 The breeding water was exchanged by transferring larvae to breeding containers prepared in advance every other day.

餌料としてアルテミアノープリウスを5個体/mlになるように投与し、メガロパ期以降にはさらに冷凍サクラエビの細片を併用した。 Artemia naprius was administered as a feed at 5 individuals / ml, and frozen cherry shrimp strips were further used after the megalopa period.

ゾエア第1期から稚ガニ2齢期まで飼育した。 The animals were raised from the first stage of Zoair to the second stage of juvenile crabs.

<結果>
SC区の表層水区、海洋深層水区ともに実験開始74〜94日後に稚ガニ2齢期に成長した。それらの生残率は、表層水区で15%、海洋深層水区で20%であった。N区では、表層水区の生残率は0%で、海洋深層水区のみ81日後に稚ガニ2齢期に成長し、その生残率は1.7%であった。SC区において、ゾエア第1期から稚ガニ2齢期までの生残率が極端に高かった(χ検定, P<0.01)。従来の飼育方法ではその生残率は0.4%であるから、大幅な生残率の向上が認められた。更に、表層水区に比較して海洋深層水区において生存率が高かった。
<Result>
Both the surface water zone and the deep ocean water zone in the SC zone grew in the second infancy of juvenile crab 74 to 94 days after the start of the experiment. Their survival rate was 15% in the surface water zone and 20% in the deep ocean water zone. In N Ward, the survival rate of the surface water zone was 0%, and only the deep sea water zone grew after the first 81 days of juvenile crab, and the survival rate was 1.7%. In the SC ward, the survival rate from the first stage of zoea to the second stage of juvenile crab was extremely high (χ 2 test, P <0.01). In the conventional breeding method, the survival rate was 0.4%, so a significant improvement in the survival rate was recognized. Furthermore, the survival rate was higher in the deep sea water area than in the surface water area.

Claims (6)

アミノグリコシド系抗生物質とクロラムフェニコール系抗生物質とが存在する飼育水中で、初期成長段階のタカアシガニを飼育することを特徴とするタカアシガニ幼体の飼育方法。   A method for rearing a hawk crab juvenile, comprising breeding a hawk crab at an initial growth stage in a breeding water in which an aminoglycoside antibiotic and a chloramphenicol antibiotic are present. 飼育水1リットルあたり、前記アミノグリコシド系抗生物質が0.04〜0.07gの範囲内で、前記クロラムフェニコール系抗生物質が0.01〜0.03gの範囲内で、それぞれ存在することを特徴とする請求項1に記載のタカアシガニ幼体の飼育方法。 The aminoglycoside antibiotic is present in the range of 0.04 to 0.07 g and the chloramphenicol antibiotic is present in the range of 0.01 to 0.03 g per liter of breeding water. The method for breeding a hawk crabs juvenile according to claim 1, characterized in that: 前記アミノグリコシド系抗生物質はストレプトマイシンであることを特徴とする請求項1又は2に記載のタカアシガニ幼体の飼育方法。 The method for breeding a giant crabs juvenile according to claim 1 or 2, wherein the aminoglycoside antibiotic is streptomycin. 前記初期成長段階は、
ゾエア期、
メガロパ期、
稚ガニ期、
ゾエア期とメガロパ期、
メガロパ期と稚ガニ期、又は
ゾエア期とメガロパ期と稚ガニ期
であることを特徴とする請求項1〜3のいずれかに記載のタカアシガニ幼体の飼育方法。
The initial growth stage includes
Zoea period,
Megalopa period,
Juvenile crab,
Zoea and Megalopa,
The method for rearing a hawk crabs larvae according to any one of claims 1 to 3, which is in a megalopa period and a juvenile crab period, or a zoea period, a megalopa period, and a juvenile crab period.
前記飼育水は海洋深層水であることを特徴とする請求項1〜4のいずれかに記載のタカアシガニ幼体の飼育方法。   The method for breeding a giant crabs juvenile according to any one of claims 1 to 4, wherein the breeding water is deep ocean water. 請求項1〜5のいずれかの飼育方法により飼育されたタカアシガニ幼体。   The hawk crab juvenile reared by the breeding method according to claim 1.
JP2006351298A 2006-12-27 2006-12-27 Method for rearing immature of macrocheira kaempferi, and immature of macrocheira kaempferi reared by the method Pending JP2008161075A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011096484A1 (en) * 2010-02-03 2011-08-11 財団法人微生物化学研究会 Read through inducer, and therapeutic agent for nonsense-mutation-type genetic diseases
CN103719003A (en) * 2013-12-25 2014-04-16 靳职雄 River crab breeding method
CN104145859A (en) * 2014-07-28 2014-11-19 苏州市新泾村农业基地专业合作社 Hairy crab breeding method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003199456A (en) * 2002-09-12 2003-07-15 Akito Yamamoto Apparatus for culturing fish and method therefor
JP2005143329A (en) * 2003-11-12 2005-06-09 Japan Science & Technology Agency Method for asepticizing zoo- and phytoplankton and method for culturing rotifer using the same method for asepticization

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003199456A (en) * 2002-09-12 2003-07-15 Akito Yamamoto Apparatus for culturing fish and method therefor
JP2005143329A (en) * 2003-11-12 2005-06-09 Japan Science & Technology Agency Method for asepticizing zoo- and phytoplankton and method for culturing rotifer using the same method for asepticization

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011096484A1 (en) * 2010-02-03 2011-08-11 財団法人微生物化学研究会 Read through inducer, and therapeutic agent for nonsense-mutation-type genetic diseases
US9358246B2 (en) 2010-02-03 2016-06-07 Microbial Chemistry Research Foundation Readthrough inducing agent and drug for treating genetic disease caused by nonsense mutation
CN103719003A (en) * 2013-12-25 2014-04-16 靳职雄 River crab breeding method
CN104145859A (en) * 2014-07-28 2014-11-19 苏州市新泾村农业基地专业合作社 Hairy crab breeding method

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