JP2008149287A - Material classification apparatus and method - Google Patents

Material classification apparatus and method Download PDF

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JP2008149287A
JP2008149287A JP2006341676A JP2006341676A JP2008149287A JP 2008149287 A JP2008149287 A JP 2008149287A JP 2006341676 A JP2006341676 A JP 2006341676A JP 2006341676 A JP2006341676 A JP 2006341676A JP 2008149287 A JP2008149287 A JP 2008149287A
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flow path
substance
size
plate
cover glass
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JP4876891B2 (en
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Norifumi Ikeda
憲文 池田
Koichi Morita
公一 森田
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NSK Ltd
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a material classification apparatus and method, which have a simple constitution, and enable a size classification with a high precision. <P>SOLUTION: A passage 2 becoming gradually shallower toward the downstream side while inclining the bottom is formed on a glass plate (passage substrate) 1. A cover glass 3 covers so as to occlude a part of the passage 2 in the passage direction. Scale marks m<SB>1</SB>-m<SB>5</SB>are attached to the cover glass 3 at a predetermined interval along the passage. When a sample solution is flowed from the upstream side end (passage starting edge 2b) of the passage 2, a collection is carried out from what has a big size in turn, and the collection of what has a smaller size is carried out at the downstream side. The size of the collected material s can be discriminated from the scale mark of the cover glass 3. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

本発明は、溶液中の微粒子や不純物の捕集又は検出に用いられ、特に細胞やバクテリア、生体高分子（例えば、タンパクやＤＮＡ）等の生体物質をサイズ分級するのに好適な物質分級装置及び物質分級方法に関する。   The present invention is used for collecting or detecting fine particles and impurities in a solution, and particularly suitable for classifying biological materials such as cells, bacteria, biopolymers (for example, proteins and DNA) and the like. It relates to a substance classification method.

溶液中の物質を分級するための技術として、例えば特許文献１〜３に示す技術が知られている。特許文献１では、マイクロ流路中に収容したサイズの異なる粒子に、交流電場を与えることにより粒子を選別している。また、特許文献２には、マイクロ流路に電極を配列し、粒子の電荷特性により粒子を選別する方法が記載されている。また、特許文献３には、サイズの異なる粒子を含む溶液の流路に、粒子のサイズに応じて異なる層流を形成することで、粒子を分級する方法が記載されている。
特開２００４−２４３２３８号公報 特開平５−１２６７９６号公報 特開２００５−２０５３８７号公報 As a technique for classifying substances in a solution, for example, techniques shown in Patent Documents 1 to 3 are known. In Patent Document 1, particles are selected by applying an alternating electric field to particles of different sizes accommodated in a microchannel. Patent Document 2 describes a method in which electrodes are arranged in microchannels and particles are selected based on the charge characteristics of the particles. Patent Document 3 describes a method of classifying particles by forming different laminar flows according to the size of particles in a flow path of a solution containing particles of different sizes.
JP 2004-243238 A JP-A-5-126696 JP 2005-205387 A

しかしながら、電界を利用する特許文献１及び２の方法では、流路に電界を形成するための電極を設ける必要がある他、分級が粒子の電荷特性に依存して行われるため、サイズによる分級を目的とする場合は精度の良い結果が得られなかった。また、分級できる物質が荷電粒子に限定されるという問題もあった。   However, in the methods of Patent Documents 1 and 2 that use an electric field, it is necessary to provide an electrode for forming an electric field in the flow path, and classification is performed depending on the charge characteristics of the particles. When intended, accurate results could not be obtained. There is also a problem that substances that can be classified are limited to charged particles.

また、特許文献３に開示されているような方法では、流路構造が複雑になるほか、送流を形成させるため流体の供給源（ポンプ）が２系統必要であり、かつ、流速や流量の厳密な制御を行う必要がある。
本発明は、上述のような問題点に鑑みてなされたものであり、簡便な構成でかつ精度の高いサイズ分級が可能な物質分級装置及び物質分級方法を提供することをその目的とする。 In addition, the method disclosed in Patent Document 3 complicates the flow path structure, requires two systems of fluid supply sources (pumps) to form a flow, and has a flow rate and flow rate. Strict control is required.
The present invention has been made in view of the above-described problems, and an object of the present invention is to provide a substance classification apparatus and a substance classification method capable of size classification with a simple configuration and high accuracy.

上記課題を解決するために、本発明の請求項１による物質分級装置は、表面に沿って延びる凹状の流路を形成した流路基板と、前記流路基板に重ねられ、前記流路の流路方向の一部を覆う板と、を備え、前記流路は下流側に向うに従って浅くなっており、前記板と前記流路の底面との間に前記流路中を流れる物質の捕集空間を形成する物質分級装置であって、流路方向に沿って付され、前記物質が捕集される流路方向の位置に対応して当該物質のサイズを特定可能な目盛を備えることを特徴とする。   In order to solve the above-described problem, a substance classification apparatus according to claim 1 of the present invention includes a flow path substrate in which a concave flow path extending along a surface is formed, and is superimposed on the flow path substrate. A plate that covers a part of the path direction, and the flow path becomes shallower toward the downstream side, and a collection space for the substance flowing in the flow path between the plate and the bottom surface of the flow path A material classifying device for forming a material, comprising a scale that is attached along a flow path direction and that can specify a size of the material corresponding to a position in the flow path direction where the substance is collected. To do.

このような構成の物質分級装置においては、溶液中の物質の径が、板と流路の底面との間の距離（以下、「ギャップ」と記す。）とほぼ同等の大きさであると捕集される。ギャップは、流路方向の位置に応じた大きさとなることから、物質が捕集された流路方向の位置によって捕集される物質のサイズを特定できる。物質のサイズに直接相関するのはギャップの大きさであるが、流路方向に沿って目盛を付す方が、流路基板を水平に配置する場合に見やすくなり、また、多くの場合、ギャップの大きさよりも捕集空間の流路方向の長さの方が大きくなるので、サイズを判別する精度が高くなる。   In the substance classifier having such a configuration, the diameter of the substance in the solution is approximately equal to the distance between the plate and the bottom surface of the channel (hereinafter referred to as “gap”). Be collected. Since the gap has a size corresponding to the position in the flow path direction, the size of the substance to be collected can be specified by the position in the flow path direction where the substance is collected. It is the size of the gap that directly correlates with the size of the substance, but the scale along the channel direction is easier to see when the channel substrate is placed horizontally, and in many cases the gap Since the length of the collection space in the flow path direction is larger than the size, the accuracy of determining the size is increased.

本発明の請求項２による物質分級装置は、請求項１において、前記流路は、底面が前記流路基板の基板面に対し傾斜面として形成されていることを特徴とする。
本発明の請求項３による物質分級装置は、請求項１又は２において、前記目盛は、前記流路の下流側端部を基準とした流路方向の任意の位置に付されていることを特徴とする。
本発明の請求項４による物質分級装置は、請求項１〜３のいずれかにおいて、前記目盛を前記板又は前記流路基板に付したことを特徴とする。 A substance classification apparatus according to claim 2 of the present invention is characterized in that, in claim 1, the flow path has a bottom surface formed as an inclined surface with respect to the substrate surface of the flow path substrate.
The substance classification apparatus according to claim 3 of the present invention is the substance classification apparatus according to claim 1 or 2, wherein the scale is attached at an arbitrary position in the flow path direction with reference to the downstream end of the flow path. And
A substance classification apparatus according to a fourth aspect of the present invention is characterized in that, in any one of the first to third aspects, the scale is attached to the plate or the flow path substrate.

本発明の請求項５による物質分級装置は、請求項１〜４のいずれかにおいて、前記板を流路方向に沿ってスライド可能にするスライド機構を備えることを特徴とする。
本発明の請求項６による物質分級装置は、請求項５において、前記スライド機構は、前記流路方向に沿って前記板を駆動する駆動装置を備えることを特徴とする。
本発明の請求項７による物質分級装置は、請求項５又は６において、前記スライド機構は、前記流路方向に沿って前記板を案内する案内部を備えることを特徴とする。 A substance classification device according to a fifth aspect of the present invention is characterized in that, in any one of the first to fourth aspects, a slide mechanism that enables the plate to slide along the flow path direction is provided.
The substance classification apparatus according to a sixth aspect of the present invention is the substance classification apparatus according to the fifth aspect, wherein the slide mechanism includes a drive device that drives the plate along the flow path direction.
A substance classification apparatus according to a seventh aspect of the present invention is characterized in that, in the fifth or sixth aspect, the slide mechanism includes a guide portion that guides the plate along the flow path direction.

本発明の請求項８による物質分級方法は、表面に沿って延びる凹状の流路を形成した流路基板と、前記流路基板に重ねられ、前記流路の流路方向の一部を覆う板と、を備え、前記流路は下流側に向うに従って浅くなっており、前記板と前記流路の底面との間に前記流路中を流れる物質の捕集空間を形成する物質分級装置を用いた物質分級方法であって、前記物質が捕集された流路方向の位置に基づいて、その捕集された物質のサイズを特定することを特徴とする。
本発明の請求項９による物質分級方法は、請求項８において、前記捕集空間に物質を捕集後、捕集された目的の物質を解放する位置まで前記板を上流側にスライドさせて、前記目的の物質を回収することを特徴とする。 A substance classification method according to an eighth aspect of the present invention includes a flow path substrate in which a concave flow path extending along a surface is formed, and a plate that is superimposed on the flow path substrate and covers a part of the flow path in the flow path direction. The flow path is shallower toward the downstream side, and a substance classifying device that forms a collection space for the substance flowing in the flow path between the plate and the bottom surface of the flow path is used. The method for classifying substances is characterized in that the size of the collected substance is specified based on the position in the flow path direction where the substance is collected.
The substance classification method according to claim 9 of the present invention is the substance classification method according to claim 8, wherein after collecting the substance in the collection space, the plate is slid upstream to a position where the collected target substance is released, The target substance is recovered.

本発明によれば、簡便な装置によって、精度の高いサイズ分級を行うことができる。   According to the present invention, highly accurate size classification can be performed with a simple apparatus.

次に、図面を参照して本発明の実施の形態である実施例１〜実施例３について説明する。
［第１実施例］
（構成）
図１（ａ）は第１実施例の物質捕集装置Ａの側面図、（ｂ）は平面図であり、図２は物質捕集装置Ａに流路２を形成する方法を示す図、図３は流路２の構造を説明するための図である。 Next, Examples 1 to 3 which are embodiments of the present invention will be described with reference to the drawings.
[First embodiment]
(Constitution)
FIG. 1A is a side view of the substance collection device A of the first embodiment, FIG. 1B is a plan view, and FIG. 2 is a diagram showing a method for forming the flow path 2 in the substance collection device A. 3 is a diagram for explaining the structure of the flow path 2.

図１に示す物質捕集装置Ａは、流路２を形成したガラス板（１０×２０ｍｍ、厚さ１ｍｍ）１に、カバーガラス３を重ねて形成した。このガラス板１が本発明の流路基板に相当し、カバーガラス３が本発明の板に相当する。
流路２は、図２に示すようにして作製した。まず、ガラス板１の長手方向片側端部の裏面側に厚さ５００μｍのＳｉウエハを敷き、ガラス板１を水平面に対して傾斜させた状態で固定した。このとき、ガラス板１は約１．４３°（＝Ｔａｎ（０．５／２０））の傾斜勾配で固定されていることになる。この後、ダイシングソーを用いて、Ｓｉウエハを敷いた側から３００μｍの切り込み量でガラスの長手方向へ幅１００μｍの直線の溝を形成した。これにより、ガラス板１表面に、底面が流路方向に沿って約１．４３°傾斜した直線溝状の流路２が約１２ｍｍの長さで形成される。 The substance collection apparatus A shown in FIG. 1 is formed by overlapping a cover glass 3 on a glass plate (10 × 20 mm, thickness 1 mm) 1 on which a flow path 2 is formed. The glass plate 1 corresponds to the flow path substrate of the present invention, and the cover glass 3 corresponds to the plate of the present invention.
The flow path 2 was produced as shown in FIG. First, a Si wafer having a thickness of 500 μm was laid on the back side of one end portion in the longitudinal direction of the glass plate 1, and the glass plate 1 was fixed while being inclined with respect to the horizontal plane. At this time, the glass plate 1 is fixed at an inclination of about 1.43 ° (= Tan (0.5 / 20)). Thereafter, using a dicing saw, a straight groove having a width of 100 μm was formed in the longitudinal direction of the glass with a cutting amount of 300 μm from the side on which the Si wafer was laid. As a result, a straight groove-like channel 2 having a bottom surface inclined by about 1.43 ° along the channel direction is formed on the surface of the glass plate 1 with a length of about 12 mm.

カバーガラス３には、市販のカバーガラス（８×１２ｍｍ、厚さ０．１２〜０．１７ｍｍ）を用い、流路２の流路終端２ａ（流路２の底が浅くなる方の端部）から上流側に０．２ｍｍの位置で固定した。流路２底面の傾斜が約１．４３°の場合、流路２の深さと流路終端２ａからの距離とは表１に示す関係にある。このため、流路２の底面とカバーガラス３の下流側端部間のギャップＧは、約５μｍ（＝０．２×（Ｔａｎ１．４３°））となる（図３参照）。また、このカバーガラス３には、流路方向に沿って所定間隔（例えば、１．５ｍｍ間隔）で目盛線ｍ１〜ｍ５を付した。 A commercially available cover glass (8 × 12 mm, thickness 0.12 to 0.17 mm) is used for the cover glass 3, and the channel end 2 a of the channel 2 (the end of the channel 2 whose bottom is shallower) And fixed upstream at a position of 0.2 mm. When the inclination of the bottom surface of the flow path 2 is about 1.43 °, the depth of the flow path 2 and the distance from the flow path end 2a are in the relationship shown in Table 1. For this reason, the gap G between the bottom face of the flow path 2 and the downstream end of the cover glass 3 is about 5 μm (= 0.2 × (Tan1.43 °)) (see FIG. 3). In addition, scale lines m 1 to m 5 were attached to the cover glass 3 at predetermined intervals (for example, 1.5 mm intervals) along the flow path direction.

Figure 2008149287
Figure 2008149287

流路始端２ｂ側へ、サンプル溶液を導入するためのチューブ５（外径約３００μｍ）を固定し、サンプル溶液が漏れない様、チューブ５の周辺をＰＤＭＳ（ｐｏｌｙｄｉｍｅｔｈｙｌｓｉｌｏｘａｎｅ）でシールした。また、ガラス板１上の流路終端２ａ側に、流路終端２ａをＰＤＭＳにより作製した側壁４ａで取り囲み、廃液を回収する回収用チャンバ４を形成した。   A tube 5 (outside diameter of about 300 μm) for introducing the sample solution was fixed to the flow path start end 2b side, and the periphery of the tube 5 was sealed with PDMS (polydimethylsiloxane) so that the sample solution did not leak. Further, on the side of the flow path end 2a on the glass plate 1, the flow path end 2a is surrounded by a side wall 4a made of PDMS, thereby forming a recovery chamber 4 for recovering waste liquid.

（作用及び効果）
次に、上記構成の物質捕集装置Ａの使用方法や作用効果について説明する。
物質捕集装置Ａの使用時には、流路始端２ｂからポンプによってサンプル溶液を流路２に送り出す。流路２の途中、カバーガラス３の固定部分では、サンプル溶液中の物質のうち、カバーガラス３と流路２の底面間のギャップＧと略同径の物質が挟まれ、捕集される。ギャップＧの大きさは、流路２の深さに相当し、流路２底面の傾斜により下流側に向かうに従って小さくなっているため、サイズの小さな物質ほど下流側で捕集されることとなり、表１に示すように流路方向の位置と捕集物質のサイズが相関する。したがって、図１（ａ）及び（ｂ）に示すように種々のサイズの物質を流した場合には、捕集物質ｓがそのサイズに応じて流路方向に分布し、その物質のサイズは、流路方向に沿って所定間隔に付されたカバーガラス３の目盛線によって知ることができる。例えば、表１に示す関係にある場合、流路終端２ａから３．２ｍｍの位置に付された目盛線ｍ２付近にある物質は、外径が８０μｍ前後であることが確認できる。 (Function and effect)
Next, the usage method and effect of the substance collection apparatus A having the above configuration will be described.
When the substance collection device A is used, the sample solution is sent out from the flow path start end 2b to the flow path 2 by a pump. In the middle of the flow path 2, among the substances in the sample solution, a substance having substantially the same diameter as the gap G between the cover glass 3 and the bottom surface of the flow path 2 is sandwiched and collected. The size of the gap G corresponds to the depth of the flow path 2 and becomes smaller toward the downstream side due to the inclination of the bottom surface of the flow path 2, so that a substance having a smaller size is collected on the downstream side, As shown in Table 1, the position in the flow path direction correlates with the size of the collected substance. Accordingly, as shown in FIGS. 1A and 1B, when substances of various sizes are flowed, the trapping substance s is distributed in the flow direction according to the size, and the size of the substance is It can be known from the scale lines of the cover glass 3 attached at predetermined intervals along the flow path direction. For example, in the case of the relationship shown in Table 1, it can be confirmed that the substance in the vicinity of the graduation line m 2 provided at a position of 3.2 mm from the channel end 2a has an outer diameter of about 80 μm.

サンプル溶液を流し終わった後は、ガラス板１やカバーガラス３が透明部材であるので、ガラス板１にカバーガラス３を被せたまま目的のサイズの物質を顕微鏡で観察することもできるし、捕集物質ｓの中から目的のサイズの物質ｓを取り出してもよい。目的の物質ｓを取り出す場合には、ポンプからの送液を停止し、カバーガラス３を取り外す。このとき、ガラス板１とカバーガラス３の間に残った溶液を下流側からスポイトなどで吸い取り、除去した後に、カバーガラス３を取り外すと、取り外し時に下流側の捕集物質ｓが上流側に流れ出したりするのを防止でき、捕集時の状態を保存したまま、目的の物質を取り出すことができる。また、ガラス板１を裏返すなどして残液を除去すると、図１（ｃ）に示すように捕集時の状態を維持して、目盛線ｍが付されたカバーガラス３の側に捕集物質ｓを取り出すことができるので、サイズを正確に特定した状態で取り出すことができる。   After flowing the sample solution, the glass plate 1 and the cover glass 3 are transparent members, so that the target size substance can be observed with a microscope while the glass plate 1 is covered with the cover glass 3. You may take out the substance s of the target size from the collection material s. When taking out the target substance s, the liquid feeding from a pump is stopped and the cover glass 3 is removed. At this time, if the solution remaining between the glass plate 1 and the cover glass 3 is sucked and removed from the downstream side with a dropper or the like and then removed, when the cover glass 3 is removed, the downstream collection substance s flows out to the upstream side at the time of removal. The target substance can be taken out while preserving the state at the time of collection. Moreover, when the residual liquid is removed by turning the glass plate 1 upside down, the state at the time of collection is maintained as shown in FIG. 1 (c), and the collection is performed on the cover glass 3 side with the graduation line m attached. Since the substance s can be taken out, it can be taken out in a state where the size is specified accurately.

なお、ギャップＧよりも外径が小さな物質は、流路終端２ａまで流されて、回収用チャンバ４内に回収される。
以上のように、カバーガラス３の流路方向に沿って流路方向の位置に応じた目盛線を付すことで、捕集物質のサイズを特定できるので、種々の物質の混合溶液をサイズ分級したり、目的の物質のサイズを特定したり、目的サイズの物質を分離したりすることができる。物質のサイズに直接相関するのはギャップの大きさであるが、このように、流路方向に沿って目盛を付す方が、流路基板を水平に配置するので見やすく、また、ギャップの大きさよりも捕集空間の流路方向の長さの方が大きいので、サイズを判別する精度が高くなる。 Note that a substance having an outer diameter smaller than the gap G flows to the flow path end 2 a and is collected in the collection chamber 4.
As described above, since the size of the collected substance can be specified by attaching a scale line corresponding to the position in the flow path direction along the flow path direction of the cover glass 3, the mixed solution of various substances is classified. Or the size of the target substance can be specified, or the target size substance can be separated. It is the size of the gap that directly correlates with the size of the material, but it is easier to see the scale along the flow path direction because the flow path substrate is placed horizontally, as compared to the size of the gap. In addition, since the length of the collection space in the flow path direction is larger, the accuracy of determining the size becomes higher.

なお、第１実施例では、流路方向に沿って所定間隔で目盛線ｍ１〜ｍ５を付したが、これに限らず、例えば、目的の物質のサイズに応じた流路方向位置にのみ目盛線を付してもよい。また、第１実施例では、目盛線を付しているだけだが、目盛線と併せて、当該目盛線の位置により特定される物質の外径サイズの数値を付したりしてもよい。 In the first embodiment, the scale lines m 1 to m 5 are attached at predetermined intervals along the flow path direction. However, the present invention is not limited to this, and for example, only the position in the flow path direction according to the size of the target substance. A scale line may be attached. In the first embodiment, only the scale line is attached, but a numerical value of the outer diameter size of the substance specified by the position of the scale line may be attached together with the scale line.

［第２実施例］
次に、第２実施例について説明する。
図４に、第２実施例の物質捕集装置Ａを示す（図４（ａ）は側面図、（ｂ）は（ａ）の物質捕集装置Ａのカバーガラス３を上流側へスライドさせた場合の平面図である）。第２実施例の物質捕集装置Ａは、第１実施例とほぼ同様の構成であるが、本発明の駆動装置である圧電素子をカバーガラス３に接続し、カバーガラス３を流路方向に沿ってスライド可能にした点が異なっている。以下、この異なる点を中心に説明し、その他の部分は説明を省略する。 [Second Embodiment]
Next, a second embodiment will be described.
FIG. 4 shows a substance collection apparatus A of the second embodiment (FIG. 4A is a side view, and FIG. 4B is a slide of the cover glass 3 of the substance collection apparatus A of FIG. 4A to the upstream side. Is a plan view of the case). The substance collection device A of the second embodiment has substantially the same configuration as that of the first embodiment, but the piezoelectric element which is the driving device of the present invention is connected to the cover glass 3 and the cover glass 3 is directed in the flow path direction. It is different in that it can slide along. Hereinafter, the description will be focused on the different points, and description of the other parts will be omitted.

図４の物質捕集装置Ａでは、ガラス板１の板厚方向に突出するリブ６が、当該ガラス板１の表面側の周囲に沿って形成されている。なお、リブ６は、ガラス板１の短手方向の一端側であって、流路２の上流側では未形成となっている。また、反対の端部側に形成され部分は、流路終端２ａから流出する溶液の回収用チャンバ４を形成する。カバーガラス３は、上記のように圧電素子に接続された状態でガラス板１の表面に重ねられており、リブ６に案内され、流路方向に沿ってスライド可能になっている。リブ６は、本発明の案内部に相当し、例えば、樹脂材料、金属材料やガラスで形成できる。リブ６と圧電素子によって本発明のスライド機構が構成されている。なお、流路２の構造や形成方法、ガラス板１及びカバーガラス３の材質や大きさ、カバーガラス３に所定間隔の目盛線が付されている点等は、第１実施例と同じである。   In the substance collection device A of FIG. 4, ribs 6 protruding in the thickness direction of the glass plate 1 are formed along the periphery on the surface side of the glass plate 1. The rib 6 is not formed on one end side in the short direction of the glass plate 1 and on the upstream side of the flow path 2. Further, the portion formed on the opposite end side forms a recovery chamber 4 for the solution flowing out from the channel end 2a. The cover glass 3 is superimposed on the surface of the glass plate 1 in a state of being connected to the piezoelectric element as described above, guided by the rib 6, and slidable along the flow path direction. The rib 6 corresponds to the guide portion of the present invention, and can be formed of, for example, a resin material, a metal material, or glass. The slide mechanism of the present invention is constituted by the rib 6 and the piezoelectric element. The structure and forming method of the flow path 2, the material and size of the glass plate 1 and the cover glass 3, and the fact that scale lines with a predetermined interval are attached to the cover glass 3 are the same as in the first embodiment. .

次に、上記構成の物質捕集装置Ａの使用方法や作用効果について説明する。
物質捕集装置Ａの使用時には、カバーガラス３を駆動して、目的物質のサイズに応じ当該物質を捕集できる位置にカバーガラス３の位置を設定する。つまり、第２実施例では、カバーガラス３の位置を変更することで、１つの物質捕集装置Ａで種々のサイズの物質の捕集に対応できる。カバーガラス３の位置を設定したら、サンプル溶液をポンプで送り出す。これにより、第１実施例と同様に、ギャップとほぼ同径の物質ｓが、カバーガラス３と流路２の底面との間に捕集される（図４（ａ）参照）。捕集物質ｓのサイズは、カバーガラス３に付された目盛線ｍ１〜ｍ５によって確認できる。なお、第２実施例では、カバーガラス３の位置を調整することで、流路終端２ａからの位置が変わるので、カバーガラス３の調整後に流路終端２ａからの位置を確認しておく必要がある。 Next, the usage method and effect of the substance collection apparatus A having the above configuration will be described.
When using the substance collection device A, the cover glass 3 is driven, and the position of the cover glass 3 is set at a position where the substance can be collected according to the size of the target substance. In other words, in the second embodiment, by changing the position of the cover glass 3, the collection of substances of various sizes can be handled by the single substance collection device A. After setting the position of the cover glass 3, the sample solution is pumped out. As a result, as in the first embodiment, the substance s having the same diameter as the gap is collected between the cover glass 3 and the bottom surface of the flow path 2 (see FIG. 4A). The size of the trapping substance s can be confirmed by the scale lines m 1 to m 5 attached to the cover glass 3. In the second embodiment, the position from the channel end 2a is changed by adjusting the position of the cover glass 3. Therefore, after the cover glass 3 is adjusted, it is necessary to confirm the position from the channel end 2a. is there.

サンプル溶液を流し終わった後は、ガラス板１やカバーガラス３が透明部材であるので、ガラス板１にカバーガラス３を被せたまま目的のサイズの物質を顕微鏡で観察することもできるし、捕集物質ｓの中から目的のサイズの物質ｓを取り出してもよい。
目的の物質ｓを取り出す場合には、ポンプからの送液を停止し、圧電素子で駆動し、目的の捕集物質ｓを解放する位置までカバーガラス３をスライドさせる。これを図４（ｂ）により具体的に説明する。図４（ｂ）は、あらかじめサイズが特定された目的物質をサンプル溶液から捕集する例である。この場合、サンプル溶液を流す前に、目的物質ｓ１（図中の黒塗りの丸）がカバーガラス３の下流側端部付近で捕集されるようにカバーガラス３の位置を設定する。サンプル溶液を流し終わった後、捕集された目的物質ｓ１を解放する位置までカバーガラス３をスライドさせ、そのままの状態でポンプから再び送液すると、解放した部分の目的物質ｓ１のみが回収用チャンバ４に送り出される。これにより、回収用チャンバ４から目的物質ｓ１を回収できる。なお、上流側からポンプで送液する代わりに、スポイトやポンプでなどで下流側から溶液を吸引し、目的物質ｓ１を回収してもよい。 After flowing the sample solution, the glass plate 1 and the cover glass 3 are transparent members, so that the target size substance can be observed with a microscope while the glass plate 1 is covered with the cover glass 3. You may take out the substance s of the target size from the collection material s.
In order to take out the target substance s, liquid feeding from the pump is stopped, and the cover glass 3 is slid to a position where the target collection substance s is released by driving with a piezoelectric element. This will be specifically described with reference to FIG. FIG. 4B shows an example in which a target substance whose size is specified in advance is collected from a sample solution. In this case, before flowing the sample solution, the position of the cover glass 3 is set so that the target substance s1 (black circle in the figure) is collected near the downstream end of the cover glass 3. After flowing the sample solution, the cover glass 3 is slid to the position where the collected target substance s1 is released, and then fed again from the pump as it is, so that only the released target substance s1 is in the collection chamber. 4 is sent out. Thereby, the target substance s1 can be recovered from the recovery chamber 4. Instead of pumping liquid from the upstream side, the target substance s1 may be recovered by sucking the solution from the downstream side with a dropper or a pump.

以上のように、カバーガラス３を流路方向に沿ってスライド可能にすることで、種々のサイズの物質を捕集でき、さらに、捕集した物質の中から目的のサイズの物質を容易に回収できる。
なお、第２実施例では、捕集物質ｓ１のみを回収する場合について説明したが、上記操作を繰り返せば、サイズの小さなものから大きなものへ捕集物質ｓ２〜ｓ４を順次回収することができる。
また、カバーガラスのスライド方法としては、圧電素子に限らず、目的物質のサイズ（スライド距離）に応じた方法（アクチュエータ）が利用できる。 As described above, by making the cover glass 3 slidable along the flow path direction, it is possible to collect substances of various sizes, and easily collect substances of the desired size from the collected substances. it can.
In the second embodiment, the case where only the trapping substance s1 is recovered has been described. However, if the above operation is repeated, the trapping substances s2 to s4 can be sequentially recovered from the small size to the large one.
Further, the method of sliding the cover glass is not limited to the piezoelectric element, and a method (actuator) corresponding to the size (slide distance) of the target substance can be used.

［第３実施例］
次に、第３実施例について説明する。
図５に、第３実施例の物質捕集装置Ａを示す（図５（ａ）は側面図、（ｂ）は（ａ）の物質捕集装置Ａのカバーガラス３を上流側へスライドさせた場合の平面図、（ｃ）はカバーガラス３を取り外した状態の平面図である。）。第３実施例の物質捕集装置Ａは、目盛線ｍ１〜ｍ５をカバーガラス３でなく、ガラス板１の方に付した点が第２実施例と異なっている。この目盛線ｍ１〜ｍ５は、印刷やエッチングなどの方法でガラス板１の裏面側に、流路終端２ａから所定距離に付されている。この目盛線ｍ１〜ｍ５は、ガラス板１及びカバーガラス３が透明であるので、表側からでも確認できる。なお、その他の部分は第２実施例と同様であるので説明を省略する。 [Third embodiment]
Next, a third embodiment will be described.
FIG. 5 shows a substance collection apparatus A of the third embodiment (FIG. 5A is a side view, and FIG. 5B is a slide of the cover glass 3 of the substance collection apparatus A of FIG. 5A to the upstream side. (C) is a plan view with the cover glass 3 removed). The material collection device A of the third embodiment is different from the second embodiment in that the scale lines m 1 to m 5 are attached not to the cover glass 3 but to the glass plate 1. The scale lines m 1 to m 5 are attached to the rear surface side of the glass plate 1 by a method such as printing or etching at a predetermined distance from the channel end 2a. The scale lines m 1 to m 5 can be confirmed even from the front side because the glass plate 1 and the cover glass 3 are transparent. Since other parts are the same as those of the second embodiment, description thereof is omitted.

以上のように、目盛線ｍ１〜ｍ５をガラス板１側に付すと、サンプル溶液を流し終わった後、カバーガラス３をスライドさせても目盛線ｍ１〜ｍ５が移動しないので、目的のサイズを正確に把握して、捕集物質ｓ１を回収できる（図５（ｂ）参照）。
なお、流路２に残った溶液を下流側からスポイトなどで吸い取り、除去すれば、図５（ｃ）に示すようにカバーガラス３を完全に取り外してもサイズ分級した状態を維持できるので、この状態から目的物質を回収してもよい。
また、図６に示すように、目盛線が付された台座７をガラス板１の下に敷いて、サイズの判定を行ったりしてもよい。 As described above, when the scale lines m 1 to m 5 are attached to the glass plate 1 side, the scale lines m 1 to m 5 do not move even if the cover glass 3 is slid after the sample solution has finished flowing. The collected material s1 can be recovered by accurately grasping the size (see FIG. 5B).
If the solution remaining in the flow path 2 is sucked and removed from the downstream side with a dropper or the like, the size-classified state can be maintained even if the cover glass 3 is completely removed as shown in FIG. The target substance may be recovered from the state.
Further, as shown in FIG. 6, the size may be determined by laying a pedestal 7 with a graduation line under the glass plate 1.

（ａ）は本実施形態の物質捕集装置の側面図、（ｂ）は平面図である。(A) is a side view of the substance collection apparatus of this embodiment, (b) is a top view. 物質捕集装置に流路を形成する方法を示す図である。It is a figure which shows the method of forming a flow path in a substance collection apparatus. 流路２の構造を説明するための図である。FIG. 3 is a diagram for explaining the structure of a flow path 2. （ａ）は第２実施例の物質捕集装置の側面図、（ｂ）は（ａ）の物質捕集装置のカバーガラスを上流側へスライドさせた場合の平面図である。(A) is a side view of the substance collection apparatus of 2nd Example, (b) is a top view at the time of sliding the cover glass of the substance collection apparatus of (a) to an upstream side. （ａ）は第３実施例の物質捕集装置の側面図、（ｂ）は（ａ）の物質捕集装置のカバーガラス３を上流側へスライドさせた場合の平面図、（ｃ）は（ａ）の物質捕集装置のカバーガラスを取り外した状態の平面図である。(A) is a side view of the material collection device of the third embodiment, (b) is a plan view when the cover glass 3 of the material collection device of (a) is slid upstream, and (c) is ( It is a top view of the state which removed the cover glass of the substance collection apparatus of a). 目盛線付きの台座の使用状態を説明するための図である。It is a figure for demonstrating the use condition of the base with a scale line.

符号の説明Explanation of symbols

１ ガラス板
２ 流路
２ａ 流路終端
２ｂ 流路始端
３ カバーガラス
４ 回収用チャンバ
４ａ 側壁
５ チューブ
６ リブ
Ａ 物質捕集装置
Ｇ ギャップ
ｍ 目盛線 DESCRIPTION OF SYMBOLS 1 Glass plate 2 Channel 2a Channel end 2b Channel start 3 Cover glass 4 Collection chamber 4a Side wall 5 Tube 6 Rib A Material collection device G Gap m Scale line

Claims (9)

表面に沿って延びる凹状の流路を形成した流路基板と、
前記流路基板に重ねられ、前記流路の流路方向の一部を覆う板と、を備え、
前記流路は下流側に向うに従って浅くなっており、前記板と前記流路の底面との間に前記流路中を流れる物質の捕集空間を形成する物質分級装置であって、
流路方向に沿って付され、前記物質が捕集される流路方向の位置に対応して当該物質のサイズを特定可能な目盛を備えることを特徴とする物質分級装置。 A flow path substrate formed with a concave flow path extending along the surface;
A plate overlaid on the flow path substrate and covering a part of the flow path direction of the flow path,
The flow path is shallower toward the downstream side, and is a substance classification device that forms a collection space for a substance flowing in the flow path between the plate and the bottom surface of the flow path,
A substance classification apparatus comprising a scale attached along a flow path direction and capable of specifying a size of the substance corresponding to a position in the flow path direction where the substance is collected. 前記流路は、底面が前記流路基板の基板面に対し傾斜面として形成されていることを特徴とする請求項１に記載の物質分級装置。   The substance classification device according to claim 1, wherein a bottom surface of the channel is formed as an inclined surface with respect to a substrate surface of the channel substrate. 前記目盛は、前記流路の下流側端部を基準とした流路方向の任意の位置に付されていることを特徴とする請求項１又は２に記載の物質分級装置。   3. The substance classification device according to claim 1, wherein the scale is attached to an arbitrary position in a flow path direction with reference to a downstream end portion of the flow path. 前記目盛を、前記板又は前記流路基板に付したことを特徴とする請求項１〜３のいずれかに記載の物質分級装置。   The substance classification device according to claim 1, wherein the scale is attached to the plate or the flow path substrate. 前記板を流路方向に沿ってスライド可能にするスライド機構を備えることを特徴とする請求項１〜４のいずれかに記載の物質分級装置。   The substance classification apparatus according to any one of claims 1 to 4, further comprising a slide mechanism that allows the plate to slide along a flow path direction. 前記スライド機構は、前記流路方向に沿って前記板を駆動する駆動装置を備えることを特徴とする請求項５に記載の物質分級装置。   The substance classification device according to claim 5, wherein the slide mechanism includes a driving device that drives the plate along the flow path direction. 前記スライド機構は、前記流路方向に沿って前記板を案内する案内部を備えることを特徴とする請求項５又は６に記載の物質分級装置。   The substance classification device according to claim 5, wherein the slide mechanism includes a guide unit that guides the plate along the flow path direction. 表面に沿って延びる凹状の流路を形成した流路基板と、
前記流路基板に重ねられ、前記流路の流路方向の一部を覆う板と、を備え、
前記流路は下流側に向うに従って浅くなっており、前記板と前記流路の底面との間に前記流路中を流れる物質の捕集空間を形成する物質分級装置を用いた物質分級方法であって、
前記物質が捕集された流路方向の位置に基づいて、その捕集された物質のサイズを特定することを特徴とする物質分級方法。 A flow path substrate formed with a concave flow path extending along the surface;
A plate overlaid on the flow path substrate and covering a part of the flow path direction of the flow path,
The flow path is shallower toward the downstream side, and is a material classification method using a material classification device that forms a collection space for a substance flowing in the flow path between the plate and the bottom surface of the flow path. There,
A material classification method, wherein the size of the collected substance is specified based on a position in a flow path direction where the substance is collected. 前記捕集空間に物質を捕集後、捕集された目的の物質を解放する位置まで前記板を上流側にスライドさせて、前記目的の物質を回収することを特徴とする請求項８に記載の物質分級方法。   The material according to claim 8, wherein after collecting the substance in the collection space, the plate is slid upstream to a position where the collected target substance is released to collect the target substance. Substance classification method.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11165062A (en) * 1997-12-02 1999-06-22 Natl Food Res Inst Laminated microchannel array device, filtration and classification method using the device and production of emulsion
JP2004181272A (en) * 2002-11-29 2004-07-02 Nomizu Kosan:Kk Metal filter
JP2004283828A (en) * 2003-03-21 2004-10-14 Steag Microparts Gmbh Microstructured separation device and microfluidic method for separating liquid component from liquid containing particle
JP2005211759A (en) * 2004-01-28 2005-08-11 Hitachi High-Technologies Corp Classification filter and blood cell separation/recovery device using the same
JP2008151658A (en) * 2006-12-18 2008-07-03 Nsk Ltd Material collection device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11165062A (en) * 1997-12-02 1999-06-22 Natl Food Res Inst Laminated microchannel array device, filtration and classification method using the device and production of emulsion
JP2004181272A (en) * 2002-11-29 2004-07-02 Nomizu Kosan:Kk Metal filter
JP2004283828A (en) * 2003-03-21 2004-10-14 Steag Microparts Gmbh Microstructured separation device and microfluidic method for separating liquid component from liquid containing particle
JP2005211759A (en) * 2004-01-28 2005-08-11 Hitachi High-Technologies Corp Classification filter and blood cell separation/recovery device using the same
JP2008151658A (en) * 2006-12-18 2008-07-03 Nsk Ltd Material collection device

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