CN108628351A - Microfluidic droplet generating means - Google Patents
Microfluidic droplet generating means Download PDFInfo
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- CN108628351A CN108628351A CN201710160825.3A CN201710160825A CN108628351A CN 108628351 A CN108628351 A CN 108628351A CN 201710160825 A CN201710160825 A CN 201710160825A CN 108628351 A CN108628351 A CN 108628351A
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D5/00—Control of dimensions of material
- G05D5/04—Control of dimensions of material of the size of items, e.g. of particles
- G05D5/06—Control of dimensions of material of the size of items, e.g. of particles characterised by the use of electric means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
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Abstract
The smaller Microfluidic droplet generating means of ratio very fast, at low cost and unqualified drop that the invention discloses a kind of governing speeds.A kind of Microfluidic droplet generating means include micro-fluidic chip, vibrating mechanism, light detecting mechanism, storing mechanism, human-computer interaction mechanism and control mechanism.The Microfluidic droplet generating means combination automatic feedback and voltage controlled oscillation regulation technology, it is capable of the size of the drop of automatic adjusument generation, the stable and higher drop of uniformity can be generated, and there is faster governing speed and drop formation speed, and adjustment process does not interfere with the generation of drop, it can serialization adjusting.Thus, drop is generated using above-mentioned Microfluidic droplet generating means, the quality for generating drop can be improved, and advantageously reduces the manufacturing cost of drop.
Description
Technical field
The present invention relates to micro fluidic chip technical fields, more particularly, to a kind of Microfluidic droplet generating means.
Background technology
Microfluidic droplet technology is a kind of completely new control very low volume fluids to grow up on the basis of micro-fluidic
Technology.The technology generate drop be nanoliter even picoliters volume micro- reaction member, oneself through applied to crystallization of protein, cell
The fields such as analysis, kinetics of fast enzyme reaction research, digital pcr and gene sequencing.Microfluidic droplet platform being capable of fast and stable
Generation drop of uniform size, compared with traditional Microdilution plate method, the screening flux of Microfluidic droplet technology can improve
1000 times, therefore, Microfluidic droplet technology has huge potentiality to become follow-on ultra-high throughput screening platform.
The generating mode of drop mainly has Y types channel method and flow focusing method etc. in micro-fluidic chip at present, makes continuous
Phase fluid makes the dispersed phase fluid forward position shrink and deforms and unstability from infall " extruding " dispersed phase fluid forward position, to
Form discrete droplets.Make as shown in Figure 1, under the action of ambient pressure in fluid channel shearing occurs for continuous phase and dispersed phase
With, two intersect exit generate drop.
Fluid channel is generally relatively narrow in micro-fluidic chip, and magnitude scale usually even lower in micron is limited to technique
With the influence of environment, it is easy to happen blocking or the larger situation of runner error, can lead to result generation occur with a collection of chip
It has a long way to go or even the consequences such as drop can not be generated.
In order to solve this problem traditional regulation method, can be detected in drop formation rear end plus CCD camera etc., root
Drop size is calculated according to testing result and readjusts continuous phase and dispersed phase end pressure source.But this kind of method presence is much asked
Topic.The adjustment of pressure source first easily lead to adjustment it is improper, (such as continuous phase viscosity is relatively low) can not generate liquid in some cases
Drop；Continuous phase and dispersed phase velocity ratio can be adjusted by adjusting pressure source simultaneously, easily cause the waste of continuous phase, this is in drop formation number
Amount is more, the cost of drop formation is improved when speed is high and continuous phase cost is higher, and common adjusting method is anti-when adjusting
Should be relatively slow, quickly drop size cannot be made to reach desired value.
Invention content
Based on this, it is necessary to provide the miniflow that a kind of governing speed is very fast, ratio at low cost and unqualified drop is smaller
Draining drips generating means.
A kind of Microfluidic droplet generating means, including micro-fluidic chip, vibrating mechanism, light detecting mechanism, storing mechanism, people
Machine interaction means and control mechanism；Wherein,
The micro-fluidic chip has continuous phase input duct, dispersed phase input duct and delivery channel, the continuous phase
Input duct and the delivery channel are connected to the dispersed phase input duct, and circulate in the dispersed phase input duct
Dispersed phase can be sheared by the continuous phase to circulate in the continuous phase input duct to be formed drop and is exported from the input duct；
The vibrating mechanism connect to drive the micro-fluidic chip vibration with the micro-fluidic chip；
The light detecting mechanism is used to detect the dimension information of drop in the delivery channel；
The storing mechanism is stored with or amplitude and/or frequency for storing the corresponding vibrating mechanism of various sizes of drop
The fluid information of rate and continuous phase and dispersed phase；
The human-computer interaction mechanism for show and input the dimension information of drop, the amplitude of vibrating mechanism and/or frequency,
And the fluid information of continuous phase and dispersed phase；
The control mechanism and the vibrating mechanism, the light-detecting structure, the storing mechanism and the human-computer interaction
Mechanism is electrically connected；The dimension information for the drop that the control mechanism is used to be inputted according to the human-computer interaction mechanism and company
The continuous amplitude and/or frequency for mutually searching the corresponding vibrating mechanism from the storing mechanism with the fluid information of dispersed phase, root
The dimension information that drop is detected according to the light detecting mechanism controls vibrating mechanism adjustment amplitude and/or frequency, described in control
The dimension information of amplitude and/or frequency and corresponding drop after storing mechanism deposit adjustment, or directly believe different sizes
The corresponding amplitude of drop and/or frequency and the fluid information of continuous phase and dispersed phase of breath are stored in the storing mechanism.
The continuous phase input duct, the delivery channel and the dispersed phase inlet flow in one of the embodiments,
Road intersects connection and forms Y types or cross.
The vibrating mechanism contacts or is embedded in the miniflow with the micro-fluidic chip in one of the embodiments,
It controls in chip.
The vibrating mechanism is located at the top of the dispersed phase input duct and close to described in one of the embodiments,
The communicating position of dispersed phase input duct and the continuous phase input duct, the vibrating mechanism and the dispersed phase input duct
The distance between be 1~3mm.
The vibrating mechanism is piezoelectric ceramic piece in one of the embodiments,.
The micro-fluidic chip corresponds to the delivery channel and is equipped with light detection window in one of the embodiments,；
The light detecting mechanism includes light source and photoelectric converter；The light source is for irradiating described light detection the window's position
The delivery channel at place；The photoelectric converter is used to detect the reflection in the delivery channel at described light detection the window's position
The picture signal of drop state is simultaneously converted to electric signal and is sent to the control mechanism；
The electric signal is converted to digital signal in the human-computer interaction mechanism and shown by the control mechanism.
The light source is one or more of LED light source, laser and mercury lamp in one of the embodiments,；It is described
Photoelectric converter is one or more of CCD, PD and PMT.
The storing mechanism is lut memory in one of the embodiments,.
The control mechanism is MCU controllers, FPGA processor and/or dsp processor in one of the embodiments,.
In one of the embodiments, the Microfluidic droplet generating means further include for press mechanism, it is described for press mechanism
For providing external pressure to the continuous phase input duct and the dispersed phase input duct.
Above-mentioned Microfluidic droplet generating means combination automatic feedback and voltage controlled oscillation regulation technology, being capable of automatic adjusument life
At drop size, can generate stablize and the higher drop of uniformity, and have faster governing speed and drop formation
Speed, and adjustment process does not interfere with the generation of drop, it can serialization adjusting.Thus, it is generated and is filled using above-mentioned Microfluidic droplet
Generation drop is set, the quality for generating drop can be improved, and advantageously reduce the manufacturing cost of drop.
Description of the drawings
Fig. 1 is the structural schematic diagram of traditional Microfluidic droplet generating means；
Fig. 2 is the structural schematic diagram of the Microfluidic droplet generating means of an embodiment of the present invention.
Specific implementation mode
To facilitate the understanding of the present invention, below with reference to relevant drawings to invention is more fully described.In attached drawing
Give the better embodiment of the present invention.But the present invention can realize in many different forms, however it is not limited to herein
Described embodiment.On the contrary, the purpose of providing these embodiments is that making understanding to the disclosure more
Add thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article and belong to the technical field of the present invention
The normally understood meaning of technical staff is identical.Used term is intended merely to description tool in the description of the invention herein
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more
Any and all combinations of relevant Listed Items.
As shown in Fig. 2, the Microfluidic droplet generating means 100 of an embodiment include micro-fluidic chip 110, vibrating mechanism
120, light detecting mechanism 130, storing mechanism 140, human-computer interaction mechanism 150 and control mechanism 160.
Micro-fluidic chip 110 has continuous phase input duct 112, dispersed phase input duct 114 and delivery channel 116.Even
Continuous phase input duct 112 and delivery channel 116 are connected to dispersed phase input duct 114, and are flowed in dispersed phase input duct 114
Logical dispersed phase can be sheared by the continuous phase to circulate in continuous phase input duct 112 to be formed drop and is exported from input duct 116.
In the present embodiment, in micro-fluidic chip 110 continuous phase input duct 112 and dispersed phase input duct 114 and
Delivery channel 116 intersects connection and forms cross-shaped configuration, and there are two continuous phase input port (or liquid storage mouth), a dispersed phase are defeated for tool
Entrance (or liquid storage mouth) and a delivery outlet are respectively used to the drop output of continuous phase, the entrance of dispersed phase and generation.It is micro-
The material of fluidic chip 110 can be any light-transmitting materials such as COC, PMMA and PC.It is understood that in other embodiments,
The shape of micro-fluidic chip 110 is without being limited thereto, such as may be the fluid channel of Y type other shapes.
Further, in the present embodiment, which also has for press mechanism, for defeated to continuous phase
Air stream enter runner 112 and dispersed phase input duct 114 provide external pressure.Continuous phase and dispersed phase flow into accordingly under external pressure
Input duct, and dispersed phase is sheared in runner by continuous phase, and drop is formed.Can be syringe pump, constant pressure pump for press mechanism
And/or air compressor etc. is capable of providing the device of pressure.The pressure can be negative pressure, can also positive pressure.
The drop generated in micro-fluidic chip 110 can be Water-In-Oil pattern, can also be oil-in-water pattern or multilayer
Package pattern.One or more surfactants can be added in continuous phase and/or dispersed phase, for enhancing the stabilization of drop
Property.
Vibrating mechanism 120 connect that micro-fluidic chip 110 is driven to vibrate with micro-fluidic chip 110.In this embodiment party
In formula, vibrating mechanism 120 is preferably placed at the top of dispersed phase input duct 114 and close to dispersed phase input duct 114 and continuously
The communicating position of phase input duct 112.The distance between vibrating mechanism 120 and dispersed phase input duct 114 are 1~3mm.Vibration
Mechanism 120 can be contacted or be embedded in micro-fluidic chip 110 with micro-fluidic chip 110.Vibrating mechanism is piezoelectric ceramic piece
Or other change vibration frequency and/or the module of amplitude according to signal frequency and/or amplitude.
Light detecting mechanism 130 is used to detect the dimension information of drop in delivery channel 116.
In the present embodiment, micro-fluidic chip 110 corresponds to delivery channel 116 and is equipped with light detection window.Light detecting mechanism
130 include light source 132 and photoelectric converter 134.Light source 132 is for the delivery channel 116 at irradiation light detection window position.Light
The light that source 132 is sent out by optical fiber or can shine directly on delivery channel 116.Photoelectric converter 134 is examined for detection light
The picture signal of reflection drop state in the delivery channel 116 surveyed at the window's position is simultaneously converted to electric signal and is sent to control machine
Structure 160, control mechanism 160 convert electrical signals to digital signal and are shown in human-computer interaction mechanism 150.Photoelectric converter 134
Corresponding picture signal can be obtained by optical fiber or direct imaging.The picture signal can be one-dimensional signal, can also be two
Dimensional signal.
Further, in the present embodiment, light source 132 can be but not limited to one in LED light source, laser and mercury lamp
Kind is several.Photoelectric converter 134 can be but not limited to one or more of CCD, PD and PMT.In addition, present embodiment
Light detecting mechanism 130 can also include electronic shutter, optical filter etc..
Storing mechanism 140 be stored with or the amplitude for storing the corresponding vibrating mechanism of various sizes of drop 120 and/or
The fluid information of frequency and continuous phase and dispersed phase.
In the present embodiment, storing mechanism 140 is preferably lut memory.Lut memory can be non-volatile readable
Memory write or other readable and writable memories retention data can not lost in the power-off state.It, can be with by lut memory
Required storage information is quickly and accurately found out, and easily the information stored will can also be needed to be stored in storing mechanism
140。
Human-computer interaction mechanism 150 is used to show and input the amplitude and/or frequency of the dimension information of drop, vibrating mechanism 120
The fluid information of rate and continuous phase and dispersed phase.Human-computer interaction mechanism 150 can be PC machine, touch screen input show equipment,
The one or more such as keyboard have the combination of information input and output function tool.
Control mechanism 160 divides with vibrating mechanism 120, light-detecting structure 130, storing mechanism 140 and human-computer interaction mechanism 150
It is not electrically connected.The dimension information and continuous phase of the drop that control mechanism 160 is used to input according to human-computer interaction mechanism 150 and divide
The fluid information of dephasing searches the amplitude and/or frequency of corresponding vibrating mechanism 120 from storing mechanism 140, according to light detection machine
The dimension information control vibrating mechanism 120 that structure 130 detects drop adjusts amplitude and/or frequency, and the control deposit of storing mechanism 140 is adjusted
The dimension information of amplitude and/or frequency and corresponding drop after whole, or it is directly that the drop of different dimension informations is corresponding
Amplitude and/or the fluid information of frequency and continuous phase and dispersed phase are stored in storing mechanism 140.
Control mechanism 160 is MCU controllers, FPGA processor and/or dsp processor, for from light detecting mechanism 130
The signal of the reflection drop state of entrance is handled to obtain the processing of drop size information and other information and/or order.
Specifically, believing by the way that the droplet size of 150 input requirements of human-computer interaction mechanism, continuous phase and dispersed phase etc. are related
Breath, control mechanism 160 are searched the relationship of corresponding droplet size and input information in storing mechanism 140 according to the information, are obtained
Electrical signal amplitude and/or frequency to driving vibrating mechanism 120 and the amplitude and frequency that adjust the electric signal, are exported by monitoring
The droplet size generated in runner 116, feeds back to control mechanism 160, and control mechanism 160 controls human-computer interaction mechanism 150 and shows
The information of current drop formation, and record current driving vibrating mechanism 120 electrical signal amplitude and/or frequency and continuous phase and/
Or the relationship of the parameters and droplet size such as dispersion phase constituent, it is compared with information is stored in storing mechanism 140, if searched not
It to corresponding informance or detects that droplet size information and the droplet size information found are inconsistent, then updates storage mechanism 140
In relevant information, while control mechanism 160 is compared according to the droplet size and desired size that detect, according to comparing knot
Fruit changes the amplitude and/or frequency of driving 120 electric signal of vibrating mechanism, to achieve the purpose that change droplet size.
By continuous phase and/or dispersed phase at gradation parameter, vibrating mechanism in device initialization or when subsequent experimental
Storing mechanism 140 is written with corresponding droplet size information in 120 vibration frequency and/or range parameter.In device initialization,
Change continuous phase and/or dispersed phase at gradation parameter, adjust the vibration frequency and/or amplitude of vibrating mechanism 120, record corresponds to
The size of drop is generated, the correspondence of droplet size and relevant parameter is recorded, storing mechanism 140 is written, or keep storage
Mechanism 140 is sky, and storing mechanism 140 is recorded in above- mentioned information when in follow-up test.
The process that the Microfluidic droplet generating means 100 of the present invention are generated with drop below in conjunction with specific embodiment is made into one
The explanation of step.
Embodiment
Piezoelectric ceramic piece is embedded in micro-fluidic chip when making micro-fluidic chip, positioned at entering for dispersed phase input duct
Mouth arrives between the intersection of continuous phase input duct, and close to intersection.Piezoelectric ceramic piece in the chip position in dispersed phase
The top of input duct is kept with dispersed phase input duct at a distance from 1~3mm.Piezoelectric ceramic piece passes through electrode and main control module
It is connected.
Assuming that the LUT records that storing mechanism has been determined by experiment have data as shown in Table 1 below, it is now desired to generate 55
The droplet of μ m diameter is matched to continuous phase component, viscosity, after disperseing the information such as phase constituent, viscosity, obtains by tabling look-up in LUT
To the corresponding vibration frequency information of 50~60 μm of liquid-drop diameter is stored in table, it is calculated under the scale, drop changes 10 μm,
Frequency probably needs to change 10kHz or so, and corresponding ratio zooms in and out, and it is attached in 25kHz to obtain vibration frequency needed for 55 μm of drops
Closely.It is 25kHz that control mechanism, which adjusts vibration frequency, and generates drop, feeds back to obtain that generate drop straight by optical de-tection means
Diameter.According to the different situations of diameter, control mechanism does following processing automatically：
1. if it find that generate liquid-drop diameter and desired value error acceptable error range (such as 0.5 μm) then not
Do any subsequent processing.
2. if it find that generate drop and error expected not within the scope of tolerable, but between 50~60 μm, then root
1kHz diameters are increased according to vibration frequency under the scale being calculated and reduce 1 μm, and correspondence increases or reduces vibration frequency, until raw
At liquid-drop diameter and desired value error in acceptable error range.
3. if it find that generating liquid-drop diameter not within 50~60 μm of sections, then it represents that occur from after system electrification work
Unusual condition, such as the runner of micro-fluidic chip occur blocking or because of technique bad reason, the chip and initialization
Chip used gap is too big when LUT tables, then it represents that data are inaccurate in the LUT tables, remove data in LUT tables, got over according to frequency
Big then smaller drop relationship selects two vibration frequencies (can rule of thumb select) to generate drop, respectively obtains corresponding drop
Size is recorded into LUT tables.Next according to circumstances 1 and 2 schemes readjust vibration frequency, until it is straight to generate drop
Diameter is with desired value error in acceptable error range.
Table 1LUT stores example
Above-mentioned Microfluidic droplet generating means 100 combine automatic feedback and voltage controlled oscillation regulation technology, can adaptively adjust
The size for saving the drop generated can generate the stable and higher drop of uniformity, and have faster governing speed and drop
Formation speed, and adjustment process does not interfere with the generation of drop, it can serialization adjusting.Thus, it is given birth to using above-mentioned Microfluidic droplet
Drop is generated at device, the quality for generating drop can be improved, and advantageously reduce the manufacturing cost of drop.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of Microfluidic droplet generating means, which is characterized in that including micro-fluidic chip, vibrating mechanism, light detecting mechanism, deposit
Store up mechanism, human-computer interaction mechanism and control mechanism；Wherein,
The micro-fluidic chip has continuous phase input duct, dispersed phase input duct and delivery channel, the continuous phase input
The dispersion that runner and the delivery channel are connected to the dispersed phase input duct, and circulate in the dispersed phase input duct
It can mutually be sheared to form drop and export from the input duct by the continuous phase to circulate in the continuous phase input duct；
The vibrating mechanism connect to drive the micro-fluidic chip vibration with the micro-fluidic chip；
The light detecting mechanism is used to detect the dimension information of drop in the delivery channel；
The storing mechanism is stored with or the amplitude for storing the corresponding vibrating mechanism of various sizes of drop and/or frequency,
And the fluid information of continuous phase and dispersed phase；
The human-computer interaction mechanism for show and input the dimension information of drop, the amplitude of vibrating mechanism and/or frequency and
The fluid information of continuous phase and dispersed phase；
The control mechanism and the vibrating mechanism, the light-detecting structure, the storing mechanism and the human-computer interaction mechanism
It is electrically connected；The dimension information and continuous phase for the drop that the control mechanism is used to be inputted according to the human-computer interaction mechanism
The amplitude and/or frequency for searching the corresponding vibrating mechanism from the storing mechanism with the fluid information of dispersed phase, according to institute
The dimension information for stating light detecting mechanism detection drop controls the vibrating mechanism adjustment amplitude and/or frequency, controls the storage
The dimension information of amplitude and/or frequency and corresponding drop after mechanism deposit adjustment, or directly by different dimension informations
The corresponding amplitude of drop and/or frequency and the fluid information of continuous phase and dispersed phase are stored in the storing mechanism.
2. Microfluidic droplet generating means as described in claim 1, which is characterized in that the continuous phase input duct, described
Delivery channel intersects with the dispersed phase input duct to be connected to form Y types or cross.
3. Microfluidic droplet generating means as described in claim 1, which is characterized in that the vibrating mechanism with it is described micro-fluidic
Chip is contacted or is embedded in the micro-fluidic chip.
4. Microfluidic droplet generating means as claimed in claim 3, which is characterized in that the vibrating mechanism is located at the dispersion
The communicating position of the top of phase input duct and close the dispersed phase input duct and the continuous phase input duct, it is described to shake
The distance between motivation structure and the dispersed phase input duct are 1~3mm.
5. Microfluidic droplet generating means as described in claim 1, which is characterized in that the vibrating mechanism is piezoelectric ceramics
Piece.
6. Microfluidic droplet generating means as described in claim 1, which is characterized in that the micro-fluidic chip corresponds to described defeated
Go out runner and is equipped with light detection window；
The light detecting mechanism includes light source and photoelectric converter；The light source is for irradiating at described light detection the window's position
The delivery channel；The photoelectric converter is used to detect the reflection drop in the delivery channel at described light detection the window's position
The picture signal of state is simultaneously converted to electric signal and is sent to the control mechanism；
The electric signal is converted to digital signal in the human-computer interaction mechanism and shown by the control mechanism.
7. Microfluidic droplet generating means as claimed in claim 6, which is characterized in that the light source is LED light source, laser
One or more of with mercury lamp；The photoelectric converter is one or more of CCD, PD and PMT.
8. Microfluidic droplet generating means as described in claim 1, which is characterized in that the storing mechanism is lut memory.
9. Microfluidic droplet generating means as described in claim 1, which is characterized in that the control mechanism be MCU controllers,
FPGA processor and/or dsp processor.
10. such as Microfluidic droplet generating means according to any one of claims 1 to 9, which is characterized in that further include for press
Structure, it is described to be used to provide external pressure to the continuous phase input duct and the dispersed phase input duct for press mechanism.
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| CN109701430A (en) * | 2019-01-21 | 2019-05-03 | 中国计量大学 | A method of vibration pipeline control T-type micro-fluidic chip generates microbubble |
| CN110052298A (en) * | 2019-05-09 | 2019-07-26 | 中国计量大学 | A method of vibration pipeline control micro-fluidic chip generates microlayer model |
| CN111729700A (en) * | 2020-07-09 | 2020-10-02 | 墨卓生物科技(上海)有限公司 | Liquid drop detection method adopting dPCR integrated chip |
| CN111729699A (en) * | 2020-07-09 | 2020-10-02 | 墨卓生物科技(上海)有限公司 | Liquid drop generating mechanism for dPCR integrated micro-fluidic chip |
| CN111760600A (en) * | 2020-06-23 | 2020-10-13 | 深圳技术大学 | Microfluidic chip, preparation method thereof and cell sorting method |
| CN113604344A (en) * | 2021-08-11 | 2021-11-05 | 北京慧智医疗器械有限公司 | High-flux integrated micro-droplet digital PCR implementation system |
| CN114308150A (en) * | 2021-12-07 | 2022-04-12 | 中国地质大学（武汉） | Feedback control type double-pulse driving liquid drop generating system and liquid drop generating method |
| CN114602368A (en) * | 2020-12-03 | 2022-06-10 | 上海远赞智造医药科技有限公司 | Droplet generating device and method |
| CN114981006B (en) * | 2020-02-03 | 2024-03-29 | 创技公司株式会社 | Microparticle production system including carrier liquid and control method thereof |
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| CN111729699A (en) * | 2020-07-09 | 2020-10-02 | 墨卓生物科技(上海)有限公司 | Liquid drop generating mechanism for dPCR integrated micro-fluidic chip |
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| CN113604344B (en) * | 2021-08-11 | 2024-03-22 | 北京慧智医疗器械有限公司 | High-flux integrated micro-droplet digital PCR (polymerase chain reaction) realization system |
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