CN108707536A - Drop formation method and system - Google Patents
Drop formation method and system Download PDFInfo
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- CN108707536A CN108707536A CN201810316477.9A CN201810316477A CN108707536A CN 108707536 A CN108707536 A CN 108707536A CN 201810316477 A CN201810316477 A CN 201810316477A CN 108707536 A CN108707536 A CN 108707536A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The present invention relates to a kind of drop formation systems, including：Air source, hold-down devices, baroceptor, pressure-regulating device and controller；The air source is connect by the pressure-regulating device with the hold-down devices；The controller is connect with the hold-down devices, the pressure-regulating device and the baroceptor.The present invention provides a kind of drop formation method and system, and it is the drop formation system of having air source, the portability of this system is considerably increased in this way, using baroceptor gathered data to the gas pressure intensity data variation of fluid chamber air inlet, utilize pid control algorithm, real-time control adjusts the pressure of pressure-regulating device output gas, the air pump output gas pressure of having air source is adjusted in conjunction with feedback real gas pressure, and then realize the pressure of stable and accurate output gas, complete the control of gas pressure intensity needed for drop formation.
Description
Technical field
The present invention relates to micro-fluidic detection technical fields, more particularly to a kind of drop formation method in micro-fluidic empty detection
And system.
Background technology
Microfluidic droplet generation technique is a kind of completely new control microbody hydrops to grow up on the basis of micro-fluidic
The technology of body.Microfluidic droplet generating platform is required to the generation drop of uniform size of fast and stable, the number of drop formation
The screening effect that amount and relationship between quality are detected to entire digital pcr.
The generating mode of drop mainly has in micro-fluidic chip at present：Y types channel method and flow focusing method etc..Utilize stream
Road pressure makes continuous phase fluid from infall " extruding " dispersed phase fluid forward position, and the dispersed phase fluid forward position is made to shrink deformation
And unstability, to form discrete droplets.The source of pressure source mainly comes from the gas of syringe pump or air pump, gas cylinder etc
The pressure controling precision in source, the gas to output of these pressure sources is relatively low, is limited in scope, and feedback mechanism is single,
Lead to the pressure transient of output gas.
Invention content
Based on this, it is necessary to it is relatively low for the precision of pressure source, it is limited in scope, and feedback mechanism is single, causes
The instability problem of pressure provides a kind of drop formation method and system.
A kind of drop formation system, including：Air source, hold-down devices, baroceptor, pressure-regulating device and control
Device；The air source is connect by the pressure-regulating device with the hold-down devices；The controller and the hold-down devices, institute
State pressure-regulating device and baroceptor connection；
The air source is used to provide the gas needed for drop formation；
The hold-down devices offer fluid chamber, can generate drop under the effect of the pressure of gas；
The gas sensor is used to detect the pressure environment in fluid chamber, generates pressure information, and the pressure is believed
Breath feeds back to the controller；
The pressure-regulating device is used to adjust the pressure for the gas that air source is exported to the hold-down devices；
The controller analyzes the atmospheric pressure value in the fluid chamber according to the pressure information that the baroceptor is fed back, and
By the atmospheric pressure value compared with generating the preset pressure value needed for drop, acquisition needs the required adjusted value of current gas pressure, and
The pressure that the pressure-regulating device adjusts the gas that the air source is exported to the hold-down devices is controlled according to the adjusted value,
So as to be maintained at preset atmospheric pressure value in the fluid chamber.
The drop formation system further includes detection device and driving device in one of the embodiments,
The detection device feeds back the location information to the controller for detecting hold-down devices location information；
The driving device is for driving hold-down devices by the operation of setting track.
The detection device is located on the running orbit of the hold-down devices in one of the embodiments,.
The hold-down devices accommodate respectively at least provided with two fluid chambers, two fluid chambers in one of the embodiments,
Different liquid.
Each described fluid chamber has air inlet and liquid outlet, the air inlet logical in one of the embodiments,
Gas pipeline is crossed with pressure-regulating device to be connected.
The gas of at least exportable two kinds different pressure of the pressure-regulating device in one of the embodiments, two kinds
The pressure of the gas of different pressure corresponds respectively to the air pressure demand of two fluid chambers, and the gas of the difference pressure passes through
Corresponding gas pipeline is transmitted in the corresponding fluid chamber.
The baroceptor is set to the gas of connection pressure-regulating device and hold-down devices in one of the embodiments,
On body pipeline.
The pressure-regulating device includes pressure regulator valve and gas circuit valve switch, the pressure regulation in one of the embodiments,
Valve inlet is connected with the air source, and outlet is connect with the gas circuit valve switch by gas pipeline.
A kind of drop formation method,
Startup is controlled the driving device, the driving device driving hold-down devices are by the operation of setting track；
Hold-down devices location information is detected, feeds back the location information to controller；
The gas of pressure is stablized in the output of control pressure regulating device, stops driving device operation；
Gas pressure intensity in detection gas pipeline and feedback information are to controller；
The pressure information fed back according to the baroceptor analyzes the atmospheric pressure value in the fluid chamber, and by the air pressure
Value is compared with generating the preset pressure value needed for drop, and acquisition needs the required adjusted value of current gas pressure, and according to the tune
Whole value controls the pressure that the pressure-regulating device adjusts the gas that the air source is exported to the hold-down devices, so that the liquid
It is endoceliac to be maintained at preset atmospheric pressure value；
Gas pipeline output stablizes the liquid in hold-down devices described in the gas extrusion of pressure in fluid chamber from liquid outlet stream
Go out.
It is described in one of the embodiments, that the pressure-regulating device output is regulated and controled according to baroceptor feedback information
Stablize the gas of pressure, regulation and control actual pressure is consistent with setting pressure, further includes：
When the actual pressure is less than setting pressure, the controller increases the air source and the pressure-regulating device
The pressure of output gas；
When the actual pressure is more than setting pressure, the controller reduces the air source and the pressure-regulating device
The pressure of output gas；
When the actual pressure is equal to setting pressure, air source is adjusted with the pressure described in the controller stability contorting
The pressure of device output gas.
The present invention provides a kind of drop formation method and system, and is the drop formation system of having air source, in this way
The portability for considerably increasing this system becomes the gas pressure intensity data of fluid chamber air inlet using baroceptor gathered data
Change, using pid control algorithm, real-time control adjusts the pressure of pressure-regulating device output gas, in conjunction with feedback real gas pressure
It emphasizes the air pump output gas pressure of section having air source, and then realizes the pressure of stable and accurate output gas, complete drop life
At the control of required gas pressure intensity.
Description of the drawings
Fig. 1 is the drop formation system module figure of one embodiment of the invention；
Fig. 2 is the drop formation method and step figure of one embodiment of the invention.
Specific implementation mode
To facilitate the understanding of the present invention, drop formation method and system is carried out more fully below with reference to relevant drawings
Description.The preferred embodiment of drop formation method and system is given in attached drawing.But drop formation method and system can be with
Many different forms are realized, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is
Keep the disclosure to drop formation method and system more thorough and comprehensive.
With reference to figure 1, the drop formation system 10 in an embodiment, including hold-down devices 110, detection device 120, driving dress
Set 130, air source 140, controller 150, pressure-regulating device 160 and baroceptor 170.The drop formation system 10 by
Power supply 20 supplies attrition voltage.The driving device 130 drives hold-down devices 110 to move, and the detection of the detection device 120 pushes
The location information is simultaneously fed back to the controller 150 in the position of device 110, and the controller 150 controls air source 140 and pressure
The gas of pressure is stablized in the output of regulating device 160, passes through gas pipeline to the hold-down devices 110.
The baroceptor 170 is located at gas pipeline close to one end of fluid chamber air inlet, or is located at fluid chamber air inlet
In mouthful, for the gas pressure intensity in detection gas pipeline, the gas pressure intensity information of acquisition is fed back into the controller 150.
The controller 150 is used to obtain 170 feedback information of the baroceptor, analyzes the feedback information, according to
The gas of the corresponding pressure of the output of analysis result control pressure regulating device 160, believes according to the position that detection device 120 is fed back
Breath starts air source 140 and pressure-regulating device 160.
Fluid chamber is offered in the hold-down devices 110, the fluid chamber offers air inlet and liquid outlet, the air inlet
Mouth is connected by gas pipeline with pressure-regulating device 160.The fluid chamber at least there are two, one of them is for accommodating water
Phase, it is another for accommodating oil phase.The gas of air pressure is stablized in the output of the pressure-regulating device 160, and the gas passes through fluid chamber
Air inlet is input in the fluid chamber, squeezes water phase or oil phase flows out to form Water-In-Oil from liquid outlet.
The air source 140, to pressure-regulating device 160, stabilization is converted into through pressure-regulating device 160 for output gas
The gas of pressure.
Specifically, the air source 140 may be selected to be the device of syringe pump, adjustable air pump, gas cylinder or other exportable gases.
The air source 140 is adjustable air pump in one of the embodiments,.
The pressure-regulating device 160 is used to export the gas of stable pressure, according to 150 control instruction tune of the controller
Control the pressure of output gas.
The pressure-regulating device 160 includes pressure regulator valve 162 and gas circuit valve switch 161 in one of the embodiments,.
162 entrance of the pressure regulator valve is connected with the air source 140, and outlet is connected with the gas circuit valve switch 161 by gas pipeline
It connects.The pressure regulator valve 162 can adjust the pressure of output gas, stablize the gas of pressure with output.The gas circuit valve switch
161 open and make to stablize the gas transport of pressure to hold-down devices 110.The pressure regulator valve 162 at least exports two kinds of different pressure
Gas is to hold-down devices 110, and to the fluid chamber for accommodating water phase, the gas of another pressure extremely accommodates the gas of one of which pressure
The fluid chamber of oil phase.
The driving device 130 is connected with the controller 150 and the hold-down devices 110, can drive pushing
Device 110 is run by setting track, and the control of acceptable control device 150.
The detection device 120 is used to detect the position of hold-down devices 110, when the hold-down devices 110 are in predetermined movement
When being moved to detection device 120 on track, the detection device 120 detects 110 location information of hold-down devices, feeds back lower press fitting
110 location informations are set to the controller 150.
Specifically, the detection device 120 may be selected to be photoelectric sensor, pressure sensor or other can acquire lower press fitting
The sensor of seated position information.
In the present embodiment, the detection device 120 is photoelectric sensor.The photoelectric sensor is mounted on the lower press fitting
The end of 110 down strokes is set, specifically, the photoelectric sensor is U-shaped Optocoupler detection device, when hold-down devices 110 move
When at photoelectric sensor, the location information is fed back to controller 150, the controller 150 obtains the letter of photoelectric sensor
Number and stop pushing according to signal control hold-down devices 110, indicate that the liquid outlet of hold-down devices 120 be to seal compact shape
State prevents air from entering.
The controller 150 obtains 170 feedback information of the baroceptor, and analyzing the feedback information control can regulating the qi flowing in the channels
The pressure of pump and 162 output gas of pressure regulator valve starts air source 140 and pressure regulation according to the location information of photo-sensor feedback
Valve 162, after opening gas circuit valve switch 161, the pressure regulator valve 162 provides the corresponding gas for stablizing pressure and exports to lower press fitting
Set 110.
The controller is opposite with the gas pressure intensity set in the controller according to the baroceptor feedback information
Than realizing voltage value output regulation and control using pid algorithm, the tune of output gas pressure being carried out to the air source and pressure-regulating device
It is whole, and then realize the compensation tuning of actual pressure so that actual pressure is close with setting pressure, awards drop formation and provides stabilization
Constant pressure guarantee.
When the actual pressure is less than setting pressure, the controller increases the air source and the pressure-regulating device
The pressure of output gas；
When the actual pressure is more than setting pressure, the controller reduces the air source and the pressure-regulating device
The pressure of output gas；
When the actual pressure is equal to setting pressure, air source is adjusted with the pressure described in the controller stability contorting
The pressure of device output gas.
Following table is the relevant test data of drop formation system：
Fig. 2 is drop formation method and step figure, and this approach includes the following steps：
Step 201, startup is controlled the driving device, driving hold-down devices are by the operation of setting track.
Step 202, hold-down devices location information, feedback position information to controller are detected.
Step 203, the gas of pressure is stablized in the output of control pressure regulating device, stops driving device operation.
Step 204, the gas pressure intensity feedback information in detection gas pipeline is to controller.
Step 205, the pressure information fed back according to the baroceptor analyzes the atmospheric pressure value in the fluid chamber, and will
The atmospheric pressure value obtains compared with generating the preset pressure value needed for drop and needs the required adjusted value of current gas pressure, and according to
The pressure that the pressure-regulating device adjusts the gas that the air source is exported to the hold-down devices is controlled according to the adjusted value, with
Make to be maintained at preset atmospheric pressure value in the fluid chamber.
Step 206, the liquid in the gas extrusion hold-down devices for stablizing pressure in fluid chamber is exported to flow out from liquid outlet.
Controller controls the driving device startup in one of the embodiments, and driving hold-down devices are by the operation of setting track.
Photoelectric sensor detects hold-down devices location status, feedback hold-down devices hold-down devices location information to controller.Controller connects
It accepts pressure device hold-down devices location information control gas circuit valve switch to open, pressure regulator valve output is made to stablize the gas of pressure, and
Stop driving device operation.Gas pressure intensity feedback information in baroceptor detection gas pipeline extremely controls.Controller according to
Baroceptor feedback information regulates and controls the gas that pressure is stablized in pressure reducing valve output, and regulation and control actual pressure is consistent with setting pressure, adopts
Real-time monitoring is carried out with pid algorithm.The liquid of water phase is accommodated in the gas extrusion hold-down devices of the stable pressure of gas pipeline output
Chamber is flowed out with the liquid in the fluid chamber for accommodating oil phase from liquid outlet.
The present invention provides a kind of drop formation method and system, and is the drop formation system of having air source, in this way
The portability for considerably increasing this system becomes the gas pressure intensity data of fluid chamber air inlet using baroceptor gathered data
Change, using pid control algorithm, real-time control adjusts the pressure of pressure-regulating device output gas, in conjunction with feedback real gas pressure
It emphasizes the air pump output gas pressure of section having air source, and then realizes the pressure of stable and accurate output gas, complete drop life
At the control of required gas pressure intensity.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of drop formation system, which is characterized in that including：Air source, hold-down devices, baroceptor, pressure-regulating device
And controller；The air source is connect by the pressure-regulating device with the hold-down devices；The controller with it is described under
Pressure device, the pressure-regulating device and baroceptor connection；
The air source is used to provide the gas needed for drop formation；
The hold-down devices offer fluid chamber, can generate drop under the effect of the pressure of gas；
The gas sensor is used to detect the pressure environment in fluid chamber, generates pressure information, and the pressure information is anti-
It is fed to the controller；
The pressure-regulating device is used to adjust the pressure for the gas that air source is exported to the hold-down devices；
The controller analyzes the atmospheric pressure value in the fluid chamber according to the pressure information that the baroceptor is fed back, and by institute
Atmospheric pressure value is stated compared with generating the preset pressure value needed for drop, acquisition needs the required adjusted value of current gas pressure, and foundation
The adjusted value controls the pressure that the pressure-regulating device adjusts the gas that the air source is exported to the hold-down devices, so that
It is maintained at preset atmospheric pressure value in the fluid chamber.
2. drop formation system according to claim 1, which is characterized in that the drop formation system further includes detection dress
It sets and driving device,
The detection device feeds back the location information to the controller for detecting hold-down devices location information；
The driving device is for driving hold-down devices by the operation of setting track.
3. drop formation system according to claim 2, which is characterized in that the detection device is located at the hold-down devices
Running orbit on.
4. drop formation system according to claim 1, which is characterized in that the hold-down devices are at least provided with two liquid
Chamber, two fluid chambers accommodate different liquid respectively.
5. drop formation system according to claim 4, which is characterized in that each described fluid chamber has air inlet
And liquid outlet, the air inlet are connected by gas pipeline with pressure-regulating device.
6. drop formation system according to claim 4, which is characterized in that the pressure-regulating device at least exportable two
The pressure of the gas of the different pressure of kind, the gas of two kinds of different pressure corresponds respectively to the air pressure demand of two fluid chambers,
The gas of the difference pressure is transmitted to by corresponding gas pipeline in the corresponding fluid chamber.
7. drop formation system according to claim 1, which is characterized in that the baroceptor is set to connection pressure
On the gas pipeline of regulating device and hold-down devices.
8. drop formation system according to claim 1, which is characterized in that the pressure-regulating device include pressure regulator valve and
Gas circuit valve switch, the pressure regulation valve inlet are connected with the air source, and outlet passes through flue with the gas circuit valve switch
Road connects.
9. a kind of drop formation method, use such as claim 1-8 any one of them drop formation systems, feature exists
In,
Startup is controlled the driving device, the driving device driving hold-down devices are by the operation of setting track；
Hold-down devices location information is detected, feeds back the location information to controller；
The gas of pressure is stablized in the output of control pressure regulating device, stops driving device operation；
Gas pressure intensity in detection gas pipeline and feedback information are to controller；
The pressure information fed back according to the baroceptor analyzes the atmospheric pressure value in the fluid chamber, and by the atmospheric pressure value with
It generates the preset pressure value needed for drop to compare, acquisition needs the required adjusted value of current gas pressure, and according to the adjusted value
The pressure that the pressure-regulating device adjusts the gas that the air source is exported to the hold-down devices is controlled, so that the fluid chamber
Interior is maintained at preset atmospheric pressure value；
Gas pipeline output stablizes the liquid in hold-down devices described in the gas extrusion of pressure in fluid chamber from liquid outlet outflow.
10. drop formation method according to claim 1, which is characterized in that described according to baroceptor feedback information
Regulate and control the gas that pressure is stablized in the pressure-regulating device output, regulation and control actual pressure is consistent with setting pressure, further includes：
When the actual pressure is less than setting pressure, the controller increases the air source and is exported with the pressure-regulating device
The pressure of gas；
When the actual pressure is more than setting pressure, the controller reduces the air source and is exported with the pressure-regulating device
The pressure of gas；
When the actual pressure is equal to setting pressure, air source and the pressure-regulating device described in the controller stability contorting
The pressure of output gas.
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| CN201810316477.9A CN108707536A (en) | 2018-04-10 | 2018-04-10 | Drop formation method and system |
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| CN201810316477.9A CN108707536A (en) | 2018-04-10 | 2018-04-10 | Drop formation method and system |
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| CN113145187A (en) * | 2021-03-06 | 2021-07-23 | 复旦大学 | Intelligent liquid drop generating system based on ROS |
| CN115254217A (en) * | 2022-07-27 | 2022-11-01 | 领航基因科技（杭州）有限公司 | Droplet preparation device and method |
| CN115254217B (en) * | 2022-07-27 | 2023-12-01 | 领航基因科技（杭州）有限公司 | Droplet preparation device and method |
| WO2024021748A1 (en) * | 2022-07-27 | 2024-02-01 | 领航基因科技（杭州）有限公司 | Droplet preparation device and method |
| US11964271B2 (en) | 2022-07-27 | 2024-04-23 | Pilot Gene Technology (hangzhou) Co., Ltd. | Drop preparation device and drop preparation method |
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