JP2008058127A - Autoanalyzer - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To solve the problem that there is a case that reproducibility lowers or the effect of a previous analytical result is exerted on a next analytical result in a case that a very small amount of a liquid sample is repeatedly analyzed, especially when a substance strongly adsorbed on the inner wall of a flow channel is contained in a specimen in the measurement of a chemical component contained in a liquid sample such as blood, urine, etc. and this phenomenon is actualized especially when the sucked sample is a very small amount of 500 ml or below or in a case that the inner diameter of the flow channel is 1 mm or below. <P>SOLUTION: When a nozzle is inserted in a liquid holding container, a series of analyzing cycles can be performed while fixing the positional relation from the nozzle to a detection part. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an automatic analyzer for analyzing components contained in a sample derived from a living body such as blood and urine, and more particularly to an automatic analyzer suitable for analyzing a trace amount sample.

  In an automatic analyzer that analyzes components contained in a sample derived from a living body such as blood or urine, as described in Patent Document 1, for example, a supply unit that supplies a plurality of types of test objects, and the supply In general, the apparatus includes a nozzle for sucking a predetermined amount of the inspection object held in the container and a drive mechanism for moving the nozzle. In a series of cycles of the analysis operation, first, the nozzle is moved using a drive mechanism to insert the nozzle into the container of the supply unit, and the liquid is sucked into the nozzle. In addition, since the nozzle is commonly used for a plurality of types of inspection objects, the nozzle is moved to the cleaning liquid supply source after every measurement or after several measurements so that the inspection objects do not mix. In general, the cleaning liquid is sucked through the nozzle and the flow path to clean the nozzle, the flow path, and the like.

  In order to ensure the degree of freedom of nozzle movement, a flexible tube, for example, a fluororesin or silicon rubber tube is used for the flow path.

Japanese Patent Laid-Open No. 10-30651

  When analyzing an inspection object using such a system, there is not much problem if the amount of the inspection object sucked into the nozzle is relatively large, but the inspection object becomes smaller as the amount of the inspection object decreases. It has been found that the suction accuracy of the is reduced. Further, it has been found that there are cases where the reproducibility is lowered even when a substance to be strongly adsorbed on the inner wall of the flow path is included in the inspection object. In particular, such a phenomenon becomes apparent when the sample to be sucked is a very small amount of 500 microliters or less, or when the inner diameter of the flow path is 1 mm or less.

  As a result of examining this cause, since the conventional automatic analyzer uses a flexible flow path, when the nozzle is moved, the shape of the flow path changes. It was estimated that the reproducibility was lowered due to changes in the flow velocity distribution and the adsorption / desorption state of the solution components on the inner surface of the flow path.

  An object of the present invention is to provide an automatic analyzer that can avoid a reduction in reproducibility of a suction amount of a trace amount liquid sample due to a change in flow path shape caused by movement of a nozzle.

  In order to solve the above-described problems, in the present invention, at least when the nozzle is inserted into the liquid holding container, a series of analysis cycles are executed while the positional relationship from the nozzle to the detection unit is fixed. We propose an automatic analyzer that can be used.

  As a first method, when the liquid surface and the nozzle are brought into contact with each other in order to suck the liquid, the nozzle and the detection unit are moved by moving the container without moving any flow path from the nozzle to the detection unit. The system was constructed by performing a series of analysis cycles while keeping the positional relationship between and fixed.

  As a second method, in order to suck the liquid, when the liquid surface and the nozzle are brought into contact with each other, the flow path from the nozzle to the detection unit is fixedly held, and the nozzle and the detection are constantly moved. A system was constructed by performing a series of analysis cycles while keeping the positional relationship with the unit constantly fixed.

  According to the present invention, it is possible to analyze a trace amount liquid sample with good reproducibility, and a more reliable analysis result can be obtained.

  Embodiments of the present invention will be described below with reference to the drawings.

  FIG. 2 schematically illustrates a system configuration according to the first embodiment. At least two containers, that is, a sample solution or a mixed solution 112 containing a sample and a cleaning liquid 114 stored in another container 113 are fixed to a movable sample stage 121. The sample stage 121 may be a rack type or a turntable type. The containers 111 and 113 can be moved up and down by a vertical movement mechanism 131. The vertical movement mechanism 301 includes at least two members, that is, a member 132 for fixing the container 111 or 113 and a drive source 133 for vertical movement. Above the member 132, there is a nozzle 141 for sucking liquid. The nozzle 141 is connected by a detection unit 151 and a flow path 161 having a fixed shape. The detection unit 151 is connected to a valve 171 and a flow path 162, and the flow path 162 is connected to one of the pump 181 and the waste liquid container 191 by switching the valve.

  A series of operation examples in the present embodiment will be described below. First, the sample solution or the mixed solution 112 containing the sample stored in the container 111 is moved directly below the nozzle 141 using the movable sample stage 121. Subsequently, after the container 111 is vertically pushed up by the vertical movement mechanism 131, the liquid is sucked using the nozzle 141, the valve 171, and the pump 181, and the target component is analyzed by the detection unit 151. Thereafter, the flow path 162 is connected to the waste liquid container 191 by switching the valve 171, and the analyzed liquid is discharged to the waste liquid container 191 using the pump 171.

  Next, the cleaning liquid 114 stored in another container 113 is moved directly below the nozzle 141 using the movable sample table 121 using the movable sample table 121. Subsequently, after the container 113 is pushed up vertically by the vertical movement mechanism 131, liquid is sucked using the nozzle 141, the valve 171, and the pump 181 to perform a cleaning operation. Thereafter, the flow path 162 is connected to the waste liquid container 191 by switching the valve 171, and the cleaning liquid 114 is discharged to the waste liquid container 191 using the pump 181.

  According to this embodiment, it is possible to perform a series of operations from suction to discharge while the positional relationship from the nozzle to the detection unit is always fixed. Can be avoided.

  Here, in the above-mentioned Japanese Patent Application Laid-Open No. 7-43368, it is pointed out that when the nozzle is moved after the reagent is aspirated, the reagent may be scattered from the vibration of the nozzle and impair accurate analysis. According to the embodiment, since the suction operation can be performed without moving the nozzle, the reagent scattering can be avoided without using the vibration suppressing member.

  FIG. 3 schematically illustrates a system configuration according to the second embodiment. At least two containers, a sample solution or a mixed solution 212 containing a sample and a cleaning liquid 214 stored in another container 213, stored in the container 211, are fixed to the sample table 221. The sample stage 221 may be a movable type such as a rack or a turntable, or may be a fixed type.

  The nozzle 231 is connected to the movable arm 251 provided with the detection unit 241 by a flow path 261 having a fixed shape. The movable arm 251 is connected to the arm drive mechanism 252 by a support column 253, and the movable arm 251 can be moved in the in-plane direction and the vertical direction, and can be rotated about the support column 253 as a central axis. The arm drive mechanism 252 includes at least two members: a drive source 254 in the vertical direction and a drive source 256 having a driving force for linear movement or rotational movement in the in-plane direction, or both. The detection unit 241 is connected to a valve 271 and a flow path 262, and the flow path 262 is connected to one of the pump 281 and the waste liquid container 291 by switching the valve.

  A series of operation examples in the present embodiment will be described below. First, the movable arm 251 is moved directly above the container 211 using the arm drive mechanism 252. Subsequently, after the movable arm 251 is moved directly below by the arm drive mechanism 252, the liquid is sucked using the nozzle 231, the valve 271, and the pump 281 and the target component is analyzed by the detection unit 241. . Thereafter, the flow path 262 is connected to the waste liquid container 291 by switching the valve 271, and the analyzed liquid is discharged to the waste liquid container 291 using the pump 281.

  Next, the movable arm 251 is moved directly above the container 213 using the arm driving mechanism 252. Subsequently, after the movable arm 251 is moved directly below by the arm driving mechanism 252, the cleaning liquid 214 is sucked using the nozzle 231, the valve 271, and the pump 281 and then the cleaning operation is performed. Thereafter, the flow path 262 is connected to the waste liquid container 291 by switching the valve 271, and the cleaning liquid 214 is discharged to the waste liquid container 291 using the pump 281.

  Even in this embodiment, it is possible to perform a series of operations from suction to discharge while the positional relationship from the nozzle to the detection unit is always fixed, so the influence of the change in the shape of the flow path on the analysis result is avoided. it can.

1 is a diagram for explaining an outline of a system configuration in Embodiment 1. FIG. FIG. 6 is a diagram for explaining an outline of a system configuration in Embodiment 2.

Explanation of symbols

111, 113, 211, 213 ... container, 112, 212 ... sample solution or mixed liquid containing sample, 114, 214 ... cleaning solution, 121, 221 ... movable sample stage, 131, 252 ... vertical movement mechanism, 132 ... container Fixed member, 133,254 ... Vertical movement drive source, 141, 231 ... Nozzle, 151, 241 ... Detector, 161, 261 ... Fixed flow path, 162, 262 ... Flow path, 171, 271 ... Valve , 181, 281... Pump, 191, 291, waste liquid container, 253, column, 255, arm fixing member, 256, driving source for linear movement or rotational movement in the plane.

Claims (2)

A nozzle for sucking liquid;
A flow path connected to the nozzle;
A suction drive source that is connected to the flow path and applies a driving force by which the nozzle sucks liquid;
A liquid holding container that holds the liquid and into which the nozzle is inserted when the nozzle sucks the liquid;
In an automatic analyzer equipped with
At least when the nozzle is inserted into the liquid holding container, a moving mechanism is provided for moving the liquid holding container so that the nozzle is inserted into the liquid holding container. A featured automatic analyzer. A nozzle for sucking liquid;
A flow path connected to the nozzle;
A suction drive source that is connected to the flow path and applies a driving force by which the nozzle sucks liquid;
A liquid holding container that holds the liquid and into which the nozzle is inserted when the nozzle sucks the liquid;
In an automatic analyzer equipped with
At least when the nozzle is inserted into the liquid holding container, the automatic analyzer is provided with a mechanism that holds the flow path from the nozzle to the suction drive source in a fixed manner so as to be constant. .

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