JP2008012188A - Biosensor cartridge and manufacturing method of biosensor cartridge - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biosensor cartridge reducing a burden of a user by decreasing the sampling amount of specimen necessary for measurement and easily collecting and measuring the specimen in a puncture hole without performing a movement of bringing the specimen collecting port 16a close to the puncture hole. <P>SOLUTION: A puncture instrument 14 is attached to at least one of a spacer layer 13 and a substrate 12b so as to make a hollow reaction section 16 smaller. This constitution can decrease the sampling amount of the specimen to reduce the burden of the user. Then, at least a part of the puncture instrument 14 is embeddedly fixed to the substrate 12b or the spacer layer 13 to bring the puncture position close to the specimen collecting port 16a. This constitution can facilitate to collect and measure the specimen in the puncture hole without performing the movement of bringing the specimen collecting port 16a close to the puncture hole. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a biosensor cartridge for measuring and analyzing chemical substances using a reagent housed in a hollow reaction part of a biosensor cartridge, for example.

Conventionally, for example, a biosensor in which a biosensor chip and a lancet are integrated has been disclosed (see, for example, Patent Document 1).
FIG. 9A is a perspective view of the sensor described in Patent Document 1, and FIG. 9B is an exploded perspective view of the sensor. As shown in FIG. 9, the lancet-integrated sensor 100 includes a chip body 101, a lancet 103, and a protective cover 105. The chip body 101 has a cover 101a and a substrate 101b that can be opened and closed, and an internal space 102 is formed on the inner surface of the cover 101a. The internal space 102 has a shape that allows the lancet 103 to be movably accommodated, and the lancet 103 can be exchanged by opening the cover 101a.

  The needle 104 provided at the tip of the lancet 103 can be moved in and out from an opening 102 a formed at the front end of the internal space 102 of the chip body 101 as the lancet 103 moves. The lancet 103 can be fixed to the chip body 101 by pressing both sides of the chip body 101 with a finger and pressing the projection 103a of the lancet 103, and puncture is performed in this fixed state. The protective cover 105 has a tube part 105 a into which the needle 104 is inserted, and the tube part 105 a can be accommodated inside the chip body 101 as the needle 104 moves. Therefore, in a state before use, the protective cover 105 is put on the needle 104 to protect the needle 104 and prevent the user from being accidentally injured. Note that the substrate 101b is provided with a pair of electrode terminals 106 so that it can be electrically connected to a measuring apparatus (not shown).

Therefore, in use, the protective cover 105 is removed, the lancet 103 is pushed, the needle 104 is protruded from the chip body 101, and both sides of the chip body 101 are pressed with fingers to fix the needle 104. After puncturing the subject in this state, the needle 104 is housed inside the chip body 101, and the opening 102a provided at the front end of the chip body 101 is brought close to the puncture port to collect blood.
WO02-056769 (FIG. 1)

  However, in the lancet-integrated sensor described in Patent Document 1, in the biosensor chip in which the puncture device is attached to the outside of the chip body (outside surface of the substrate), the size of the internal space can be reduced. Since the position of the puncture port through which the sample flows out after being punctured by the puncture device and the position of the sample extraction port of the biosensor chip for collecting the sample are separated from each other, a sample collection error is likely to occur. In the lancet-integrated sensor 100, since the needle 104 is movably provided in the internal space 102 between the cover 101a and the substrate 101b, the width and height of the internal space 102 are made larger than the outer diameter of the needle 104. Since it cannot be made small and the internal space 102 for accommodating the sample becomes large, the amount of sample collected becomes large, and the blood collection burden on the user increases.

  The purpose of the present invention is to reduce the burden on the user by reducing the amount of sample collected for measurement and to easily collect the sample at the puncture port without requiring the operation of bringing the sample collection port close to the puncture port. An object of the present invention is to provide a biosensor cartridge that can be measured.

  In order to achieve the above-described object, a biosensor cartridge according to the present invention includes a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and a tip of the chip body. A biosensor cartridge having a puncture device fixed to a portion and having a protruding tip, wherein at least a part of the puncture device is fixed to at least one of the substrate and the spacer layer by being embedded.

  In the biosensor cartridge thus configured, at least a part of the puncture device is fixed by being embedded in at least one of the substrate or the spacer layer, so that the hollow reaction part can be made small. Thereby, the amount of samples collected can be reduced and the burden on the user can be reduced. At this time, since at least a part of the puncture device is fixed so as to be embedded in at least one of the substrate and the spacer layer, the puncture position can be brought close to the sample collection port. As a result, the sample of the puncture port can be easily collected and measured without requiring an operation for bringing the sample collection port close to the puncture port. It is desirable to embed more than half of the puncture device. Thereby, the puncture device can be prevented from falling off the substrate or the spacer layer. In the present invention, a needle, a lancet needle, a cannula and the like are collectively referred to as a puncture device.

  In the biosensor cartridge according to the present invention, at least a part of the puncture instrument is fixed by being embedded in one of the two substrates.

  In the biosensor cartridge configured as described above, the puncture device is attached to the substrate instead of the spacer layer, so that the thickness of the spacer layer can be reduced and the hollow reaction portion provided in the spacer layer can be reduced. be able to. Thereby, the amount of samples collected can be reduced and the burden on the user can be reduced. At this time, since at least a part of the puncture device is fixed so as to be embedded in the substrate, the puncture device can be brought closer to the spacer layer, and the puncture position can be made closer to the sample collection port. As a result, the sample of the puncture port can be easily collected and measured without requiring an operation for bringing the sample collection port close to the puncture port. It is desirable to fix more than half of the puncture device by embedment. Thereby, the puncture device can be prevented from falling off the substrate.

  In the biosensor cartridge according to the present invention, at least a part of the puncture device is fixed in a groove provided in the substrate.

  In the biosensor cartridge configured as described above, at least a part of the puncture device can be easily embedded in the substrate by attaching at least a part of the puncture device to the V groove provided on the substrate. The position can be brought close to the sampling port. Note that the depth of the groove is desirably a depth at which more than half of the puncture device is embedded. Examples of the method for fixing the puncture device in the groove include fixing with an adhesive or an adhesive tape, and heating and pushing the substrate.

  The biosensor cartridge manufacturing method according to the present invention includes a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and is fixed to the tip of the chip body. A method of manufacturing a biosensor cartridge having a puncture device with a protruding tip, wherein at least a part of the puncture device is fixed by being embedded when the substrate is molded from a molten resin.

  In the biosensor cartridge manufacturing method configured as described above, since at least a part of the puncture device is fixed by being embedded in the substrate when the substrate is molded from the molten resin, the puncture is performed easily and reliably. The instrument can be embedded and fixed in the substrate. Moreover, since a plurality of aligned puncture devices can be embedded at the same time on the aligned substrates, the manufacturing efficiency is good. Thereby, the thickness of the spacer layer can be reduced, and the hollow reaction part provided in the spacer layer can be reduced, so that the amount of sample collected can be reduced and the burden on the user can be reduced. In addition, since the puncture device can be brought close to the spacer layer and the puncture position can be brought close to the sample collection port, the sample of the puncture port can be easily collected without requiring the operation of bringing the sample collection port close to the puncture port. Will be able to. It is desirable to arrange so that more than half of the puncture device is embedded. Thereby, the puncture device can be prevented from falling off the substrate.

  The biosensor cartridge manufacturing method according to the present invention includes a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and is fixed to the tip of the chip body. A biosensor cartridge manufacturing method including a puncture device having a protruding tip, wherein at least a part of the puncture device is pushed into the heat-softened substrate and fixed by embedding.

  In the method of manufacturing the biosensor cartridge configured as described above, the substrate is heated and softened, and at least a part of the puncture device is pushed in and fixed by embedding. Therefore, at least the puncture device is later attached to the manufactured substrate. Part can be easily embedded and fixed. Thereby, the thickness of the spacer layer can be reduced, and the hollow reaction part provided in the spacer layer can be reduced, so that the amount of sample collected can be reduced and the burden on the user can be reduced. In addition, since the puncture device can be brought close to the spacer layer and the puncture position can be brought close to the sample collection port, the sample of the puncture port can be easily collected without requiring the operation of bringing the sample collection port close to the puncture port. Will be able to. As a method of heat softening the substrate and spacer layer, it is preferable to heat soften only the embedded portion rather than heat softening the entire substrate and spacer layer because a sample having low heat resistance can be used as a reaction sample contained in the hollow reaction part. . As a partial heating method, it is possible to partially heat and soften the embedded portion with a laser, or to narrow the range of heat influence by resistance welding.

  The biosensor cartridge manufacturing method according to the present invention includes a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and is fixed to the tip of the chip body. A biosensor cartridge manufacturing method including a puncture device with a protruding tip, wherein a groove is formed in the substrate, and at least a part of the puncture device is fixed in the groove.

  In the biosensor cartridge manufacturing method configured as described above, since at least a part of the puncture device is fixed in the groove formed in the substrate, the puncture device can be easily embedded in the substrate. Thereby, the thickness of the spacer layer can be reduced, and the hollow reaction part provided in the spacer layer can be reduced, so that the amount of sample collected can be reduced and the burden on the user can be reduced. In addition, since the puncture device can be brought close to the spacer layer and the puncture position can be brought close to the sample collection port, the sample of the puncture port can be easily collected without requiring the operation of bringing the sample collection port close to the puncture port. Will be able to. Note that the depth of the groove is desirably a depth at which more than half of the puncture device is embedded. Moreover, as a method of fixing the puncture device in the groove, it can be fixed by heating and pressing an adhesive, an adhesive tape or a substrate.

  The biosensor cartridge manufacturing method according to the present invention includes a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and is fixed to the tip of the chip body. A biosensor cartridge manufacturing method including a puncture device having a protruding tip, wherein at least a part of the puncture device is fixed in a substrate by ultrasonic bonding.

  In the biosensor cartridge manufacturing method configured as described above, since at least a part of the puncture instrument is embedded in the substrate by ultrasonic bonding from the outside of the substrate, it is easy to reduce the influence of heating after manufacturing the substrate. A puncture device can be attached to the. Thereby, the thickness of the spacer layer can be reduced, and the hollow reaction part provided in the spacer layer can be reduced, so that the amount of sample collected can be reduced and the burden on the user can be reduced. In addition, since the puncture device can be brought close to the spacer layer and the puncture position can be brought close to the sample collection port, the sample of the puncture port can be easily collected without requiring the operation of bringing the sample collection port close to the puncture port. Will be able to. It is more effective to perform ultrasonic bonding while heating.

  A biosensor device according to the present invention is collected by connecting to the biosensor cartridge described above or a biosensor cartridge manufactured by the method of manufacturing a biosensor cartridge described above and a detection electrode of the biosensor cartridge. And a measuring instrument for obtaining information on the sample.

  In the biosensor device configured as described above, a sample is collected by the above-described biosensor cartridge, and information on the sample is transmitted to the measuring device via the detection electrode, so that the measurement can be easily performed in a short time. Therefore, the burden on the subject can be reduced.

  According to the present invention, since the puncture device is attached to the substrate or at least one of the substrate and the spacer layer, the hollow reaction part can be made small. Thereby, the amount of samples collected can be reduced and the burden on the user can be reduced. At this time, since at least a part of the puncture device is embedded in the substrate, the puncture device can be brought close to the sample collection port. Thereby, the effect that the sample of the puncture port can be easily collected and measured without requiring the operation of bringing the sample collection port close to the puncture port is obtained.

DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments according to the present invention will be described in detail with reference to the drawings.
1A is a plan view showing a first embodiment of the biosensor cartridge of the present invention, FIG. 1B is a side view seen from the direction B in FIG. 1A, and FIG. 1A is an end view as viewed from the direction C, FIG. 2A is a plan view showing a second embodiment of the biosensor cartridge of the present invention, and FIG. 2B is B in FIG. The side view seen from the direction, FIG. 2C is an end view seen from the C direction in FIG.

  As shown in FIGS. 1A to 1C, the biosensor cartridge 10 according to the first embodiment of the present invention includes two substrates 12a and 12b facing each other, and the two substrates 12a and 12b. It has a chip body 11 having a spacer layer 13 to be sandwiched, and a puncture device 14 that is fixed to the tip portion 11a of the chip body 11 and protruded from the tip 14a. At least a part of the puncture device 14 is fixed by being embedded in at least one of the two substrates 12a and 12b 12b (hereinafter, the case where the puncture device 14 is attached to the substrate 12b). ing.

  That is, the two substrates 12a and 12b have an overall rectangular shape, and can be made of, for example, polyethylene terephthalate (PET). On the surface of at least one substrate 12a of the two substrates 12a and 12b on the spacer layer 13 side, for example, detection electrodes 15a and 15b are provided by printing carbon on the substrate 12a. The parts (the lower end in FIG. 1A) are bent in an L shape in directions opposite to each other to maintain a predetermined interval. A rectangular hollow reaction portion 16 is formed by the two substrates 12 a and 12 b and the spacer layer 13 from the tip 11 a of the chip body 11 to a portion where the two detection electrodes 15 a and 15 b are opposed to each other. At the tip of the hollow reaction part 16, a sample collection port 16 a for introducing blood as a sample collected by puncturing the specimen with the puncture instrument 14 is provided in the hollow reaction part 16.

Accordingly, the hollow reaction part 16 is formed of the substrates 12a and 12b and the detection electrodes 15a and 15b on both upper and lower surfaces, and a rectangular space is formed with the spacer layer 13 cut into a predetermined shape as a side wall. For this reason, in the hollow reaction part 16, the detection electrodes 15a and 15b are exposed, for example, in the blood collected by immobilizing an enzyme and a mediator, for example, immediately above or in the vicinity of the detection electrodes 15a and 15b in the hollow reaction part 16. A reagent 17 that generates an electric current by reacting with glucose is provided. Therefore, the hollow reaction portion 16 is a portion where a sample such as blood collected from the sample collection port 16a undergoes a biochemical reaction with the reagent 17.
Further, as shown in FIGS. 1A and 1B, at the rear end portion 11b of the chip body 11, only the substrate 12a is extended, and the detection electrodes 15a and 15b are exposed.

In the biosensor cartridge 10 described above, the puncture device 14 is attached not to the spacer layer 13 but to the substrates 12a and 12b. Therefore, the thickness of the spacer layer 13 can be reduced, and the spacer layer 13 is provided. The hollow reaction part 16 can be made small. Thereby, the collection amount of the sample B can be decreased and a user's burden can be reduced. At this time, since at least a part of the puncture device 14 is embedded in the substrate 12b, the puncture device 14 can be brought close to the spacer layer 13 and the puncture position can be made close to the sample collection port 16a. As a result, the sample B at the puncture port can be easily collected and measured without requiring the operation of bringing the sample collection port 16a close to the puncture port. In addition, it is desirable to fix so that more than half of the cross section of the puncture device 14 is embedded. As a result, the puncture device 14 can be reliably prevented from falling off the substrate 12b, and the distance between the puncture position by the puncture device 14 and the sample collection port 16a can be shortened. Can be prevented.
In the biosensor cartridge according to the present invention, in addition to the above-described embodiment, at least a part of the puncture device may be fixed by being embedded in at least one of the two substrates and the spacer layer.

Next, a biosensor cartridge 10B according to a second embodiment of the present invention will be described based on FIG. In addition, the same code | symbol is attached | subjected to the site | part which is common in the biosensor cartridge 10 which concerns on 1st Embodiment mentioned above, and the overlapping description is abbreviate | omitted.
As shown in FIGS. 2A to 2C, in the biosensor cartridge 10B according to the second embodiment of the present invention, at least a part of the puncture instrument 14 is fixed to the groove 18 provided in the substrate 12b. ing. Various shapes of the groove 18 are conceivable. For example, any of a V shape, a partial arc shape, a rectangular shape, and the like are possible, but it is preferable to form in advance on the outer surface of the substrate 12b to which the puncture device 14 is attached. Further, as a method for fixing the puncture device 14, an adhesive or an adhesive tape can be used, but various manufacturing methods described later can also be applied.

  Thus, by fixing the puncture device 14 in the groove 18 formed on the outer surface of the substrate 12b, the puncture device 14 can be easily embedded in the substrate 12b at an accurate position, and the puncture position can be set. It can be brought close to the sampling port 16a. It is desirable that the depth of the groove 18 is a depth at which half or more of the cross section of the puncture device 14 is embedded. Thereby, it is possible to prevent the puncture device 14 from falling off the substrate 12b.

Next, a method for manufacturing the biosensor cartridge according to the present invention will be described.
3A and 3B show a method for manufacturing a first biosensor cartridge according to the third embodiment of the present invention. In this biosensor cartridge manufacturing method, a chip body 11 having two substrates 12a and 12b facing each other and a spacer layer 13 sandwiched between the two substrates 12a and 12b, and the chip body 11 A method of manufacturing a biosensor cartridge 10 having a puncture device 14 fixed to a distal end portion 11a and protruding from a distal end 14a, wherein at least a part of the puncture device 14 is embedded by molding a substrate 12b with a molten resin. It is to be fixed.

  That is, when the resin substrate 12b is molded from resin, the puncture device 14 is attached to a predetermined position of the mold 19 for molding the substrate 12b, and the molten resin is poured into the mold, so that at least the puncture device 14 is provided. The substrate 12b partially embedded and fixed is integrally molded.

  As shown in FIG. 3A, a plurality of molds 19 are provided in parallel to mold a plurality of substrates 12b, and a plurality of puncture devices 14 are arranged at predetermined positions of the mold 19. The state is shown. As shown in FIG. 3B, the puncture device 14 is arranged so that at least a part thereof is buried in the substrate 12b. At this time, it is desirable to arrange the puncture device 14 so that half or more of the cross section of the puncture device 14 is fixed by embedding with resin.

  As described above, in the first method for producing a biosensor cartridge, when the substrate 12b is molded from a molten resin, at least a part of the puncture instrument 14 is embedded and fixed in the substrate 12b. Therefore, the puncture device 14 can be easily and securely embedded and fixed in the substrate 12b, and more than half of the cross section of the puncture device 14 can be easily fixed by being embedded. In addition, since a plurality of puncture devices 14 aligned on the aligned substrates 12b can be embedded and fixed at a time, manufacturing efficiency is good.

Next, the manufacturing method of the 2nd biosensor cartridge which is 4th Embodiment concerning this invention is demonstrated.
In this biosensor cartridge manufacturing method, a chip body 11 having two substrates 12a and 12b facing each other and a spacer layer 13 sandwiched between the two substrates 12a and 12b, and the chip body 11 A method of manufacturing a biosensor cartridge 10 having a puncture device 14 fixed to a distal end portion 11a and protruding from a distal end 14a, wherein at least a part of the puncture device 14 is pushed and embedded in a heat-softened substrate 12b. To do.

That is, the manufactured resin substrate 12b is heated and softened, and at least a part of the puncture device 14 is pushed into the softened portion and embedded and fixed.
As a method of heating and melting the substrate 12b, resistance welding can be used as shown in FIG. That is, the positive electrode 21a and the negative electrode 21b of the resistance welding terminal 21 energize the metal puncture device 14 as an example to cause the puncture device 14 to generate heat, thereby heating and softening the resin substrate 12b. At the same time, a pressing force is applied to the puncture device 14 to push it into the resin 12b. Alternatively, as shown in FIG. 5, a high resistance metal foil 22 is interposed between the resistance welding terminal 21 and the puncture device 14 to heat the puncture device 14 more efficiently to generate heat, and the substrate is heated by the heat. 12b can also be softened.

  As described above, in this second biosensor cartridge manufacturing method, the substrate 12b is heated and softened, and at least a part of the puncture instrument 14 is pushed in and embedded, so that the manufactured substrate 12b is later The puncture device 14 can be easily embedded. Further, by heating the puncture device 14 using resistance welding to melt the substrate 12b, it can be heated in a short time in a pinpoint manner, and the adverse effect on other parts due to heating can be prevented. Can do.

Next, the manufacturing method of the 3rd biosensor cartridge which is 5th Embodiment concerning this invention is demonstrated.
In this biosensor cartridge manufacturing method, a chip body 11 having two substrates 12a and 12b facing each other and a spacer layer 13 sandwiched between the two substrates 12a and 12b, and the chip body 11 A biosensor cartridge 10 having a puncture device 14 fixed to a distal end portion 11a and protruding from a distal end 14a, wherein a groove 18 is formed in a substrate 12b, and at least a part of the puncture device 14 is formed in the groove 18. It is to be fixed.

That is, as shown in FIG. 6, the groove 18 is formed in the resin substrate 12 b in advance, or the groove 18 is formed after manufacturing, and the puncture device 14 is positioned and fixed in the groove 18.
As a method of fixing the puncture device 14 to the groove 18, an adhesive or an adhesive tape can be used. Alternatively, by using the above-described resistance welding, it is possible to simultaneously heat and melt and push at least a part of the puncture device 14 into the groove 18.

  As described above, in this third biosensor cartridge manufacturing method, since at least a part of the puncture device 14 is fixed to the groove 18 formed in the substrate 12b, the puncture device 14 can be easily attached to the substrate 12b. It can be embedded and fixed inside. Further, the depth at which the puncture device 14 is embedded can be easily adjusted by the depth of the groove 18, and more than half of the cross section of the puncture device 14 can be embedded and fixed. Furthermore, when heating and melting are performed at the same time, it is possible to prevent the resin from rising around by embedding at least a part of the puncture device 14 and to clean the finish.

Next, the manufacturing method of the 4th biosensor cartridge which is 6th Embodiment concerning this invention is demonstrated.
In this biosensor cartridge manufacturing method, a chip body 11 having two substrates 12a and 12b facing each other and a spacer layer 13 sandwiched between the two substrates 12a and 12b, and the chip body 11 A method of manufacturing a biosensor cartridge 10 having a puncture device 14 which is fixed to a tip portion 11a and protrudes from a tip 14a, wherein at least a part of the puncture device 14 is fixed in a substrate 12b by ultrasonic bonding. is there.

  That is, as shown in FIG. 7, the puncture device 14 is placed on the resin substrate 12b, the puncture device 14 is pressurized by the pressurizing means 23, and the pressurizing means 23 is vibrated in parallel by the horn 24 (FIG. 7), at least a part of the puncture device 14 is fixed in the substrate 12b. At this time, heating and melting can be performed simultaneously.

  As described above, in the fourth biosensor cartridge manufacturing method, at least a part of the puncture instrument 14 is embedded in the substrate 12b by ultrasonic bonding from the outside of the substrate 12b. The puncture device 14 can be easily embedded and fixed while reducing the influence of heating.

Next, a biosensor device according to a seventh embodiment of the invention will be described. FIG. 8 shows a configuration of a biosensor device 30 using the above-described biosensor cartridges 10 and 10B.
As shown in FIG. 8, the biosensor device 30 includes the biosensor cartridges 10 and 10B described above or the biosensor cartridges 10 and 10B manufactured by the above-described biosensor cartridge manufacturing method, And a measuring instrument 31 that obtains information on blood collected by connecting to 10B detection electrodes 15a and 15b. Note that the configuration of the biosensor cartridges 10 and 10B is as described above, and the same reference numerals are given to portions common to the biosensor cartridges 10 and 10B described above, and the description thereof is omitted here.

  The measuring device 31 includes a power supply 32, a control device 33, a terminal insertion unit 34, and a display unit 35, which are connected to each other. The rear end portion 11b of the chip body 11 of the biosensor cartridge 10 is inserted and fixed to the terminal insertion portion 34, and the detection electrodes 18a and 18b exposed at the rear end portion 11b of the chip body 11 are electrically connected. To be connected to. The biosensor device 30 is small in size, for example, a handy type that a subject can hold with one hand. Although not shown, the biosensor device 30 is provided with a puncture mechanism (lancet) that can move the chip body by a spring means or the like, and is attached to the tip of a cap 36 provided at the tip of the sensor device 30. Puncturing can be performed by pressing the finger of the person to be inspected and moving the chip body 11. After the puncture is completed, blood can be collected by a biosensor to measure a blood glucose level or the like.

  As described above, in the biosensor device 30 described above, blood is collected by the biosensor cartridges 10 and 10B described above, and blood information is transmitted to the measuring device 31 via the detection electrodes 15a and 15b. Therefore, the burden on the subject can be reduced.

The biosensor cartridge of the present invention is not limited to the above-described embodiments, and appropriate modifications and improvements can be made.
For example, in each of the above-described embodiments, the detection electrodes 15a and 15b are provided on one substrate 12a and the puncture device 14 is provided on the other substrate 12b. However, the puncture device 14 is used as the detection electrode. It is also possible to provide the same substrate 12a as 15a and 15b.

  As described above, since the biosensor cartridge according to the present invention attaches the puncture device to the substrate instead of the spacer layer, the thickness of the spacer layer can be reduced, and the hollow reaction part provided in the spacer layer is provided. Can be small. Thereby, the amount of samples collected can be reduced and the burden on the user can be reduced. At this time, since at least a part of the puncture device is fixed so as to be embedded in the substrate, the puncture device can be brought closer to the spacer layer, and the puncture position can be made closer to the sample collection port. This has the effect that the sample of the puncture port can be easily collected and measured without requiring the operation of bringing the sample collection port close to the puncture port, and the reagent contained in the hollow reaction part of the cartridge is used. It is useful as a biosensor cartridge for measuring and analyzing chemical substances.

(A) is a top view which shows 1st Embodiment which concerns on the biosensor cartridge of this invention. (B) is the side view seen from the B direction in FIG. 1 (A). (C) is the end view seen from the C direction in FIG. 1 (A). (A) is a top view which shows 2nd Embodiment which concerns on the biosensor cartridge of this invention. (B) is the side view seen from the B direction in FIG. 2 (A). (C) is the end view seen from the C direction in FIG. 2 (A). (A) is a top view which shows the method of providing the puncture instrument integrally at the time of shaping | molding of a board | substrate. (B) is a side view. It is explanatory drawing which shows an example of the method of embedding and fixing the puncture instrument by heating-softening a board | substrate by resistance welding. It is explanatory drawing which shows another example of the method of heat-softening a board | substrate by resistance welding and embedding and fixing the puncture instrument. It is explanatory drawing which shows the method to embed and fix the puncture device in the groove | channel provided in the board | substrate. It is explanatory drawing which shows the method to embed and fix the puncture instrument in a board | substrate by ultrasonic bonding. It is a block diagram of a biosensor device according to the present invention. (A) is a perspective view of a conventional biosensor. (B) is an exploded perspective view of a biosensor.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10, 10B Biosensor cartridge 11 Chip body 11a Tip part 12a, 12b Substrate 13 Spacer layer 14a Tip 14 Puncture instrument 18 Groove 30 Biosensor device 31 Measuring instrument

Claims (8)

A biosensor cartridge comprising a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and a puncture device fixed to the tip of the chip body and protruding from the tip Because
A biosensor cartridge, wherein at least a part of the puncture device is fixed by being embedded in at least one of the substrate and the spacer layer.   2. The biosensor cartridge according to claim 1, wherein at least a part of the puncture device is fixed to one of the two substrates by being embedded.   The biosensor cartridge according to claim 1 or 2, wherein the puncture device is fixed in a groove provided in the substrate. A biosensor cartridge comprising a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and a puncture device fixed to the tip of the chip body and protruding from the tip A manufacturing method of
A method for producing a biosensor cartridge, comprising: embedding and fixing at least a part of the puncture device when the substrate is molded from a molten resin. A biosensor cartridge comprising a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and a puncture device fixed to the tip of the chip body and protruding from the tip A manufacturing method of
A biosensor cartridge manufacturing method, wherein at least a part of the puncture device is pushed into the heat-softened substrate and fixed by embedding. A biosensor cartridge comprising a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and a puncture device fixed to the tip of the chip body and protruding from the tip A manufacturing method of
A method for producing a biosensor cartridge, wherein a groove is formed in the substrate, and at least a part of the puncture device is fixed in the groove. A biosensor cartridge comprising a chip body having two substrates facing each other and a spacer layer sandwiched between the two substrates, and a puncture device fixed to the tip of the chip body and protruding from the tip A manufacturing method of
A biosensor cartridge manufacturing method, wherein at least a part of the puncture device is fixed in a substrate by ultrasonic bonding.   The biosensor cartridge according to any one of claims 1 to 3, or the biosensor cartridge manufactured by the method for manufacturing a biosensor cartridge according to any one of claims 4 to 7, and the biosensor A biosensor apparatus comprising: a measuring device that obtains information on a sample collected by connecting to a detection electrode of a cartridge.

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