JP2007529215A - 異なるチャンバーにおいて電界を発生する容器及び装置 - Google Patents
異なるチャンバーにおいて電界を発生する容器及び装置 Download PDFInfo
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Abstract
【選択図】図1
Description
2 チャンバー
3 壁領域
4 第1電極
5 第2電極
6 空隙
7 接触領域
8 接触領域
9 接触領域
10 列
11 列
12 下部
13 グランド
15 容器
16 締結部材
17 フレーム
20 容器
21 チャンバー
22 壁領域
23 第1電極
24 第2電極
25 空隙
26 表面
27 開口
28 側壁
29 接触領域
30 接触領域
31 接触素子
32 構造体
33 接触素子
34 接触素子
35 装置
36 ケース
37 平板
38 接触素子
39 プラットフォーム
40 ケースの開口
41 内部空間
42 穴
45 装置
46 パルス発生器
47 蓄積部
48 蓄積部
49 蓄積部
50 蓄積部
51 エネルギー蓄積部
52 電源
55 センサ抵抗
56 電極
57 スイッチング素子
58 電極
59 制御部
60 コンピュータ
61 調節部
62 装置
63 第1電極
64 第2電極
65 スイッチング素子
Claims (24)
- 第1(4、23)および第2(5、24)電極を含む少なくとも一対の電極をそれぞれ備える各チャンバー(2、21)を備え、前記一対の電極に電圧を印加することにより1つのチャンバー(2、21)内に電界を発生させる容器(1、15、20)であって、
異なるチャンバー(2、21)の少なくとも2つの第1電極(4、23)は導電結合しており、前記チャンバー(2、21)の少なくとも1つの第2電極(5、24)は単独に導電結合可能である容器。 - 前記電圧印加のために電気的に接触可能な特定の接触領域(7、8、9、29、30)が前記電極(4、5、23、24)に割り当てられている、請求項1に記載の容器。
- 異なるチャンバー(2、21)の前記少なくとも2つの結合した電極(4、23)は、共通接触領域(8、29)によって接触可能である、請求項2に記載の容器。
- チャンバー(2、21)の前記第2電極(5、24)はそれぞれそれ専用に割り当てられた接触領域(9、30)を有する、請求項2または3に記載の容器。
- 前記接触領域(7、8、9、29、30)は、前記電極(4、5、23、24)に直接取り付けられる、請求項2、3または4に記載の容器。
- 前記接触領域(7、8、9、29、30)は、前記容器(1、15、20)のグランド(13)上の前記電極(4、5、23、24)の底面側に配置される、請求項2〜5に記載の容器。
- 複数のチャンバー(2、21)、好ましくは6、8、12、16、24、32、48、64、96、128、192、384、1536、3456または6144個のチャンバーが設けられる、請求項1〜6のいずれかに記載の容器。
- 幾つかのチャンバー(2、21)が一列または少なくとも2つの平行な列に配置され、一列の前記異なるチャンバー(2、21)の同じ側に配置される電極(4、5、23、24)の少なくとも一部が導電結合している、請求項1〜7のいずれかに記載の容器。
- 隣接する列の異なるチャンバー(2、21)の少なくとも2つの向かい合って配置される電極(4、5、23、24)が導電結合している、請求項8に記載の容器。
- 少なくとも1つの電極(4、5、23、24)は、導電性材料をドープしたポリマーからなり、特にカーボンファイバー、グラファイト、カーボンブラックおよび/またはカーボンナノチューブを好ましくは40〜80重量%のドーピング濃度でドープしている、請求項1〜9のいずれかに記載の容器。
- 前記接触領域(7、8、9、29、30)は、前記電極(4、5、23、24)に取り付けられた接触材料を備え、前記電極(4、5、23、24)の材料よりも23℃での比抵抗または境界抵抗が低い、請求項2〜10のいずれかに記載の容器。
- 前記接触材料は金属、好ましくは銅であり、または本質的に導電性の複合材料であり、および/または前記接触材料は、23℃での比抵抗が1×10-5オーム・cmより小さく、好ましくは1×10-6〜2×10-6オーム・cmである、請求項11に記載の容器。
- 請求項1〜12のいずれかに記載の容器(1、15、20)の製造方法であって、前記チャンバー(2、21)を構築し、チャンバー(2、21)毎に少なくとも2つの予備領域を備える壁領域(3)は、最初は非導電性ポリマーの射出成形により作成され、その後前記予備領域に導電性材料が付与されるかまたは、まずチャンバー(2、21)毎の少なくとも2つの領域を導電性材料で製造または成形フレーム内に配置し、次に、前記チャンバー(2、21)を構築する壁領域(3)を非導電性ポリマーの射出成形により前記少なくとも2つの領域の周辺に作成する方法であって、導電性材料を含む、異なるチャンバー(2、21)の少なくとも2つの領域は、互いに導電結合しており、チャンバー(2、21)の少なくともその他の部分はそれぞれ前記電気接触に対して単独で接続する方法。
- 電気接触のために接触材料を前記導電性材料に取り付ける、または導電性材料、好ましくは金属または本質的に導電性の複合材料を圧力および/または熱の作用、好ましくはホットエンボスにより、および/または好ましくはより低い比抵抗を有する接着層の作用により、前記導電性材料に取り付ける、請求項13に記載の方法。
- 請求項1〜12のいずれかに記載の容器(1、15、20)の少なくとも1つと電気的に接触するための装置(35)であって、前記容器(1、15、20)の1つのチャンバー(2、21)の少なくとも1つの第2電極(5、24)それぞれに対して1つの接触素子(31、34、38)がそれぞれ設けられ、前記電気的に結合した第1電極(4、23)に対して幾つかの接触素子(31、33)、好ましくは1〜6個の接触素子が設けられる装置。
- 好ましくは平板(37)である構造体(32)が設けられ、その上に前記接触素子(31、33、34、38)が配置される、請求項15に記載の装置。
- 前記構造体(32)は、特にモータによって垂直方向および/または水平方向に可動である、請求項16に記載の装置。
- 前記容器(1、15、20)を設置するためのプラットフォーム(39)が設けられ、特にモータによって水平方向および/または垂直方向に可動である、請求項15、16または17のいずれかに記載の装置。
- 前記プラットフォーム(39)は、少なくとも1つの開口を備え、または前記プラットフォーム(39)は穴開けされた平板(punched plate)であり、穴(42)の数は好ましくは接触素子(31、33、34、38)の数に対応する、請求項18に記載の装置。
- 内部空間(41)が設けられ、その中に前記接触素子(38)が配置され、および/または少なくとも1つの開口が設けられ、前記開口を介して前記接触素子(38)が挿入可能である、請求項15〜19のいずれかに記載の装置。
- ケースの開口(40)が設けられ、前記開口を介して容器(1、15、20)および/またはプラットフォーム(39)が前記内部空間(41)に挿入可能である、請求項15〜20のいずれかに記載の装置。
- 少なくとも1つの蓄積部(47、48、49、50)を備え、または少なくとも1つの蓄積部(47、48、49、50)に接続可能であって、前記蓄積部(47、48、49、50)は好ましくはコンデンサである、請求項15〜21のいずれかに記載の装置。
- 前記蓄積部(47、48、49、50)と前記容器(1、15、20)の前記電極(4、5、23、24)との間に少なくとも1つのスイッチング素子(57、65)が配置され、および/または各内部空間(2、21)および/または各結合した電極グループ(4、23)にスイッチング素子(57、65)が割り当てられ、前記スイッチング素子(57、65)は好ましくはリレーである、請求項15〜22のいずれかに記載の装置。
- 前記スイッチング素子(57、65)は、前記接触素子(31、33、34、38)に直接取り付けられ、好ましくは前記接触素子(31、33、34、38)がその上に配置される前記構造体(32)の下に取り付けられる、請求項23に記載の装置。
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EP04006058A EP1577378B1 (de) | 2004-03-15 | 2004-03-15 | Behältnis und Vorrichtung zur Erzeugung von elektrischen Feldern in einzelnen Reaktionsräumen |
EP04006058.4 | 2004-03-15 | ||
PCT/IB2005/000663 WO2005090547A1 (en) | 2004-03-15 | 2005-03-15 | Container and device for generating electric fields in different chambers |
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Cited By (6)
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---|---|---|---|---|
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JP2012515529A (ja) * | 2009-01-20 | 2012-07-12 | ロンザ ケルン ゲーエムベーハー | 反応空間の電気的処理のための方法及び装置 |
JP2012515531A (ja) * | 2009-01-20 | 2012-07-12 | ロンザ ケルン ゲーエムベーハー | 複数の容器の電気的処理のための方法及びデバイス |
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JP2017505656A (ja) * | 2014-01-17 | 2017-02-23 | ゼネラル・エレクトリック・カンパニイ | 容量結合を使用する電気パルス発生システム |
JP2022514519A (ja) * | 2018-12-13 | 2022-02-14 | フラウンホーファー-ゲゼルシャフト ツル フェルデルング デル アンゲヴァンテン フォルシュング エー ファウ | 炭素系材料製の試料レセプタクルを含む生体試料用の試料保持装置 |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6897069B1 (en) | 2004-06-08 | 2005-05-24 | Ambion, Inc. | System and method for electroporating a sample |
KR100853102B1 (ko) * | 2004-06-12 | 2008-08-21 | 주식회사 디지탈바이오테크놀러지 | 중공구조를 갖는 전기천공 장치 |
US20100019782A1 (en) * | 2005-06-29 | 2010-01-28 | Matsushita Electric Industrial Co., Ltd. | Cellular potential measurement container |
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WO2024059824A2 (en) | 2022-09-16 | 2024-03-21 | Arsenal Biosciences, Inc. | Immune cells with combination gene perturbations |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6130690A (ja) * | 1984-06-27 | 1986-02-12 | ヴエ−・ツエ−・ヘレウス・ゲゼルシヤフト・ミツト・ベシユレンクタ−・ハフツング | 複合電極、その製法及び使用方法 |
JPH02131569A (ja) * | 1988-11-11 | 1990-05-21 | Hitachi Ltd | マイクロチャンバープレート、これを利用した細胞検出方法、処理方法および装置ならびに細胞 |
US5183744A (en) * | 1988-10-26 | 1993-02-02 | Hitachi, Ltd. | Cell handling method for cell fusion processor |
WO2004010103A2 (en) * | 2002-07-20 | 2004-01-29 | Acea Biosciences, Inc. | Impedance based devices and methods for use in assays |
WO2004050866A1 (en) * | 2002-12-03 | 2004-06-17 | Genetronics, Inc. | Large-scale electroporation plates, systems, and methods of use |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4067951A (en) * | 1975-11-19 | 1978-01-10 | Bactomatic Inc. | Process for making impedance measuring module |
US4882281A (en) * | 1986-04-02 | 1989-11-21 | Jeffrey L. Hilliard | Probe for electrofusion, electroporation, or like procedure |
IL108775A (en) * | 1994-02-25 | 2003-09-17 | Univ Ramot | Method for efficient incorporation of molecules into cells |
US5563067A (en) * | 1994-06-13 | 1996-10-08 | Matsushita Electric Industrial Co., Ltd. | Cell potential measurement apparatus having a plurality of microelectrodes |
DE19917571B4 (de) | 1999-04-19 | 2014-03-20 | Robert Poppe | Elektrodenraster für Elektroporationsreaktionsansätze |
DE19928410C2 (de) * | 1999-06-22 | 2002-11-28 | Agilent Technologies Inc | Gerätegehäuse mit einer Einrichtung zum Betrieb eines Labor-Mikrochips |
US7312043B2 (en) * | 2000-07-10 | 2007-12-25 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
AU2001275881B2 (en) * | 2000-07-10 | 2006-04-06 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
DE10033578A1 (de) * | 2000-07-11 | 2002-01-24 | Emtec Magnetics Gmbh | Leitfähige Polymerzusammensetzung auf der Basis von Graphit |
US20030129716A1 (en) | 2001-12-06 | 2003-07-10 | Bio-Rad Laboratories, Inc. | Automatic electroporation optimization system |
AU2003206415A1 (en) * | 2002-01-07 | 2003-07-24 | Uab Research Foundation | Electroporation cuvette-pipette tips, multi-well cuvette arrays, and electrode template apparatus adapted for automation and uses thereof |
GB2386949A (en) | 2002-03-26 | 2003-10-01 | Sensor Tech Ltd | A multiwell plate for electrochemical detection |
US7470533B2 (en) * | 2002-12-20 | 2008-12-30 | Acea Biosciences | Impedance based devices and methods for use in assays |
US7521224B2 (en) * | 2003-09-30 | 2009-04-21 | The United States Of America As Represented By The Secretary Of The Navy | Microelectronic cell electroporation array |
US6878538B1 (en) | 2003-10-28 | 2005-04-12 | Cyto Pulse Sciences, Inc. | Apparatus for receiving and aligning a multiple electrode pair array and a multiple treatment cell for use in electroporation |
US7371561B2 (en) | 2003-10-28 | 2008-05-13 | Cyto Pulse Sciences | In vitro, multiple electrode pair array and multiple treatment cell apparatus for use in electroporation |
-
2004
- 2004-03-15 ES ES04006058T patent/ES2331003T3/es not_active Expired - Lifetime
- 2004-03-15 DE DE502004009820T patent/DE502004009820D1/de not_active Expired - Lifetime
- 2004-03-15 DK DK04006058T patent/DK1577378T3/da active
- 2004-03-15 EP EP06012478A patent/EP1702677B1/de not_active Expired - Lifetime
- 2004-03-15 ES ES06012478T patent/ES2294757T3/es not_active Expired - Lifetime
- 2004-03-15 AT AT06012478T patent/ATE376454T1/de active
- 2004-03-15 EP EP04006058A patent/EP1577378B1/de not_active Expired - Lifetime
- 2004-03-15 PL PL04006058T patent/PL1577378T3/pl unknown
- 2004-03-15 AT AT04006058T patent/ATE437940T1/de active
-
2005
- 2005-03-15 CA CA2559580A patent/CA2559580C/en active Active
- 2005-03-15 AU AU2005224452A patent/AU2005224452B2/en active Active
- 2005-03-15 KR KR1020067021294A patent/KR101077008B1/ko active IP Right Grant
- 2005-03-15 JP JP2007503439A patent/JP4680254B2/ja active Active
- 2005-03-15 CN CN2005800079084A patent/CN1930280B/zh active Active
- 2005-03-15 US US11/079,192 patent/US8101401B2/en active Active
- 2005-03-15 CN CN201210074701.0A patent/CN102641705B/zh active Active
- 2005-03-15 WO PCT/IB2005/000663 patent/WO2005090547A1/en active Application Filing
-
2010
- 2010-08-03 AU AU2010207750A patent/AU2010207750A1/en not_active Abandoned
-
2011
- 2011-02-25 AU AU2011200804A patent/AU2011200804B2/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6130690A (ja) * | 1984-06-27 | 1986-02-12 | ヴエ−・ツエ−・ヘレウス・ゲゼルシヤフト・ミツト・ベシユレンクタ−・ハフツング | 複合電極、その製法及び使用方法 |
US5183744A (en) * | 1988-10-26 | 1993-02-02 | Hitachi, Ltd. | Cell handling method for cell fusion processor |
JPH02131569A (ja) * | 1988-11-11 | 1990-05-21 | Hitachi Ltd | マイクロチャンバープレート、これを利用した細胞検出方法、処理方法および装置ならびに細胞 |
WO2004010103A2 (en) * | 2002-07-20 | 2004-01-29 | Acea Biosciences, Inc. | Impedance based devices and methods for use in assays |
WO2004050866A1 (en) * | 2002-12-03 | 2004-06-17 | Genetronics, Inc. | Large-scale electroporation plates, systems, and methods of use |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012515530A (ja) * | 2009-01-20 | 2012-07-12 | ロンザ ケルン ゲーエムベーハー | 複数の反応空間および電極を備える容器 |
JP2012515529A (ja) * | 2009-01-20 | 2012-07-12 | ロンザ ケルン ゲーエムベーハー | 反応空間の電気的処理のための方法及び装置 |
JP2012515531A (ja) * | 2009-01-20 | 2012-07-12 | ロンザ ケルン ゲーエムベーハー | 複数の容器の電気的処理のための方法及びデバイス |
JP2015027303A (ja) * | 2009-01-20 | 2015-02-12 | ロンザ ケルン ゲーエムベーハー | 複数の容器の電気的処理のための方法及びデバイス並びに接触装置 |
WO2014065330A1 (ja) * | 2012-10-25 | 2014-05-01 | 浜松ホトニクス株式会社 | 細胞観察装置、電気刺激装置、及び細胞観察方法 |
JP2015163082A (ja) * | 2012-10-25 | 2015-09-10 | 浜松ホトニクス株式会社 | 細胞観察装置及び細胞観察方法 |
US9778189B2 (en) | 2012-10-25 | 2017-10-03 | Hamamatsu Photonics K.K. | Cell observation device, electrical stimulation device, and cell observation method |
JP2017505656A (ja) * | 2014-01-17 | 2017-02-23 | ゼネラル・エレクトリック・カンパニイ | 容量結合を使用する電気パルス発生システム |
JP2022514519A (ja) * | 2018-12-13 | 2022-02-14 | フラウンホーファー-ゲゼルシャフト ツル フェルデルング デル アンゲヴァンテン フォルシュング エー ファウ | 炭素系材料製の試料レセプタクルを含む生体試料用の試料保持装置 |
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PL1577378T3 (pl) | 2010-02-26 |
CA2559580C (en) | 2012-03-13 |
US20060087522A1 (en) | 2006-04-27 |
CN1930280B (zh) | 2012-05-16 |
KR101077008B1 (ko) | 2011-10-26 |
ATE376454T1 (de) | 2007-11-15 |
AU2011200804B2 (en) | 2011-09-22 |
EP1702677B1 (de) | 2007-10-24 |
CN102641705A (zh) | 2012-08-22 |
AU2011200804A1 (en) | 2011-03-17 |
EP1577378B1 (de) | 2009-07-29 |
DE502004009820D1 (de) | 2009-09-10 |
ES2294757T3 (es) | 2008-04-01 |
WO2005090547A1 (en) | 2005-09-29 |
ATE437940T1 (de) | 2009-08-15 |
CN1930280A (zh) | 2007-03-14 |
AU2010207750A1 (en) | 2010-09-09 |
AU2005224452B2 (en) | 2011-08-04 |
US8101401B2 (en) | 2012-01-24 |
CN102641705B (zh) | 2014-11-26 |
JP4680254B2 (ja) | 2011-05-11 |
DK1577378T3 (da) | 2009-12-07 |
ES2331003T3 (es) | 2009-12-18 |
EP1577378A1 (de) | 2005-09-21 |
KR20070015171A (ko) | 2007-02-01 |
EP1702677A1 (de) | 2006-09-20 |
AU2005224452A1 (en) | 2005-09-29 |
CA2559580A1 (en) | 2005-09-29 |
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