JP2007309834A - Agitation method of sample liquid - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an agitation method of sample liquid capable of agitating easily the sample liquid by an agitation mechanism having a simple constitution and a container. <P>SOLUTION: The sample liquid is agitated by performing a process for supplying the sample liquid into a sample liquid holding part through a support port of a measuring device by using the measuring device having a prescribed constitution and a measuring device, a process for applying a voltage to the interval between a pair of electrodes, a process for measuring an electric signal from the pair of electrodes, a process for detecting that the support port is taken out from the sample liquid based on a change of the electric signal, and a process for supplying gas into the sample liquid holding part automatically through the support port based on detection, in the sample liquid holding part. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a stirring method for stirring a sample solution and a reagent.

Conventionally, as a method of stirring a sample liquid using a gas, the sample liquid is injected into the container using a liquid injection mechanism such as a pipetter, and then the gas is injected into the container using a gas injection mechanism such as a pump. There has been a method of injecting and stirring the sample liquid injected into the container (for example, see Patent Documents 1 and 2).
JP 2002-286602 A JP 2003-34878 A

However, the conventional configuration as described above has separate mechanisms for supplying liquid and gas into the container, and the liquid injection and gas injection openings are separately provided in the container. Therefore, there is a problem that the configuration of the stirring mechanism and the container becomes large and complicated.
Therefore, the present invention solves the conventional problems as described above, and provides a sample liquid stirring method capable of easily stirring a sample liquid with a stirring mechanism and a container having a simple configuration. Objective.

In order to solve the above conventional problems, the present invention provides:
A substrate, a sample solution holding unit provided inside the substrate for holding a sample solution, a supply port communicating with the sample solution holding unit and supplying the sample solution and gas to the sample solution holding unit; An opening communicating with the sample solution holding unit, a reagent provided in the sample solution holding unit and specifically reacting with a test substance contained in the sample solution, and a pair of electrodes provided on the outer surface of the substrate A measuring device comprising:
A measuring device mounting portion for mounting the measuring device by being connected to the opening of the measuring device;
A suction means for sucking the gas in the sample liquid holding part through the opening of the measurement device attached to the measurement device attachment part;
A voltage application unit for applying a voltage between the pair of electrodes of the measurement device;
An electrical signal measuring unit for measuring electrical signals from the pair of electrodes;
Using a measuring device equipped with
(A) supplying the sample solution to the sample solution holding unit through the supply port;
(B) applying a voltage between the pair of electrodes;
(C) measuring an electrical signal from the pair of electrodes;
(D) detecting the supply port taken out from the sample solution based on the change in the electrical signal;
(E) In the sample liquid holding part, based on the detection in the step (D), the method of automatically supplying the gas to the sample liquid holding part through the supply port is provided. To do.

  ADVANTAGE OF THE INVENTION According to this invention, the sample solution stirring method which can stir a sample solution easily with the stirring mechanism and container of a simple structure can be provided.

The stirring method of the present invention comprises:
A substrate, a sample solution holding unit provided inside the substrate for holding a sample solution, a supply port communicating with the sample solution holding unit and supplying the sample solution and gas to the sample solution holding unit; An opening communicating with the sample solution holding unit, a reagent provided in the sample solution holding unit and specifically reacting with a test substance contained in the sample solution, and a pair of electrodes provided on the outer surface of the substrate A measuring device comprising:
A measuring device mounting portion for mounting the measuring device by being connected to the opening of the measuring device;
A suction means for sucking the gas in the sample liquid holding part through the opening of the measurement device attached to the measurement device attachment part;
A voltage application unit for applying a voltage between the pair of electrodes of the measurement device;
An electrical signal measuring unit for measuring electrical signals from the pair of electrodes;
Using a measuring device equipped with
(A) supplying the sample solution to the sample solution holding unit through the supply port;
(B) applying a voltage between the pair of electrodes;
(C) measuring an electrical signal from the pair of electrodes;
(D) detecting the supply port taken out from the sample solution based on the change in the electrical signal;
(E) automatically supplying the gas to the sample liquid holding part through the supply port based on the detection in the step (D) in the sample liquid holding part.
According to such a method, the sample solution and the gas can be supplied to the measurement device using one suction means, so that the measurement apparatus can be simplified and downsized. Further, by configuring the supply port for supplying the sample solution and the gas to the sample solution holding part of the measurement device with the same one, the measurement device can be simplified and downsized. Furthermore, since it is detected that the supply port has been taken out of the sample liquid and the gas is automatically supplied to the sample liquid holding unit through the supply port, the gas can be easily supplied to the sample solution holding unit. Further, since the sample liquid is not accidentally sucked, there is no problem in stirring.

The stirring method of the present invention comprises:
Before the step (A),
(F) applying a voltage between the pair of electrodes;
(G) measuring an electrical signal from the pair of electrodes;
(H) detecting that the supply port is immersed in the sample liquid based on the change in the electrical signal;
Is preferably further included.
Furthermore, in the measurement device used in the stirring method of the present invention, the tip portions of the pair of electrodes and the supply port may be provided at a height that substantially matches.
In the measurement device, the supply port may be provided between a tip portion of the pair of electrodes and the opening. According to the latter, when the measuring device is taken out from the sample solution, it can be detected more reliably that the supply port has come out of the surface of the sample solution.

  Here, it is preferable that the reagent is provided in a dry state in the sample solution holding unit, and is disposed so as to dissolve in the sample solution when the sample is supplied into the sample solution holding unit. For example, a porous carrier made of glass fiber, filter paper, or the like is impregnated with a reagent solution and then dried to support the reagent, and the porous carrier is provided in the sample liquid holding portion. Further, the reagent may be arranged by directly applying the reagent solution to the wall surface constituting the sample solution holding unit and then drying the solution.

  Examples of the reagent include enzymes, antibodies, hormone receptors, chemiluminescent reagents, DNA, and the like. In particular, since antibodies can be produced by known methods, they are advantageous in that a reagent can be easily produced. For example, an antibody against the antigen can be obtained by immunizing mice, rabbits and the like using proteins such as albumin and hormones such as hCG and LH as antigens. Examples of the antibody include an antibody against a protein contained in urine such as albumin and an antibody against a hormone contained in urine such as hCG and LH.

Examples of the sample liquid in the present invention include body samples such as urine, serum, plasma, and blood, and liquid samples such as a culture supernatant. Of these, urine is preferred as a sample. When the sample is urine, daily health management at home can be performed non-invasively.
Examples of the test substance include albumin, hCG, LH, CRP, IgG, and the like.

  In the urine qualitative test performed at the first stage of health care, 12 items of pH, specific gravity, protein, sugar, occult blood, ketone body, bilirubin, urobilinogen, nitrite, leukocyte, ascorbic acid, amylase, and salt are tested. Is called. In addition, for the purpose of analyzing renal function, trace albumin is used, and markers such as pregnancy tests include hormones such as hCG and LH. For proteins, hormones such as microalbumin, hCG, and LH, optical measurement based on antigen-antibody reaction is suitable. Here, examples of the optical measurement based on the antigen-antibody reaction include an immunotrophic method, an immunoturbidimetric method, and a latex immunoaggregation method.

  Moreover, it is preferable that the measuring device of the present invention is composed of an optically transparent material or a material that substantially does not have visible light absorption except for a pair of electrodes. For example, quartz, glass, polystyrene, polymethyl methacrylate, or the like can be given. When the device is made disposable, polystyrene is preferable from the viewpoint of cost.

As a material for the electrode provided in the measurement device, a material containing at least one of gold, platinum, palladium, an alloy or a mixture thereof, and carbon is preferable. These materials are chemically and electrochemically stable, and a stable measurement can be realized.
Moreover, it is preferable that the gas supplied to the sample solution holding part in order to stir the sample solution is air. In this case, a separate tank for holding gas is not required, and simplification, downsizing, and low cost of the measuring apparatus can be realized.
Hereinafter, embodiments of the present invention will be described in more detail with reference to the drawings. In this embodiment, a case where the sample is urine and the test substance is human albumin will be described.

(1) Measuring Device First, the structure of the measuring device according to the present embodiment will be described with reference to FIGS. FIG. 1 is a perspective view showing the structure of a measuring device according to the present embodiment, and FIG. 2 is a cross-sectional view of the measuring device.
The measurement device 1 according to the present embodiment includes a first measurement device member 108 and a second measurement device member 109. By combining the first measurement device member 108 and the second measurement device member 109, A space that functions as the sample liquid holding unit 103 is formed. The sample liquid holding unit 103 has a quadrangular prism shape with a bottom surface of about 5 mm square and a height of 44 mm, for example. The measuring device 1 is also provided with an opening 101 for attaching the measuring device 1 to the measuring device attaching / detaching portion 211 of the measuring apparatus 2 and a supply port 102 for sucking a sample and air into the sample liquid holding portion 103. ing. The opening 101 and the supply port 102 communicate with the sample liquid holding unit 103, respectively. The measurement device body is provided with an optical window 104 for performing optical measurement. The supply port 102 corresponds to the supply port in the present invention.

  In addition, among the five surfaces constituting the outer surface of the measuring device 1, a pair of conductive portions 105 c and 105 d are provided on the surface where the supply port 102 is installed. These conductive portions 105 c and 105 d extend from the opening 101 to the bottom surface of the sample liquid holding unit 103. The conductive portions 105c and 105d include electrodes 105e and 105f, respectively. Furthermore, the conductive portions 105c and 105d include joint lead portions 105a and 105b, respectively. The size (length) of the electrodes 105e and 105f and the joining lead portions 105a and 105b are defined by a cover 106 made of an insulating resin. For example, the lengths of the electrode and the joining lead part are 10 mm and 10 mm, respectively. Since the tip portions of the pair of electrodes 105e and 105f extend to the bottom surface of the sample solution holding unit 103, the supply port 102 is located between the tip portion of the pair of electrodes 105e and 105f and the opening 101.

A method for manufacturing the measurement device of this embodiment will be described below with reference to FIGS. FIG. 3 is an exploded perspective view of the measuring device according to the present embodiment.
The first measurement device member 108 and the second measurement device member 109 are made of, for example, transparent polystyrene. This transparent polystyrene member can be obtained, for example, by molding using a mold. A known resin molding technique may be used for molding. The second measuring device member 109 is open at the upper and right ends and has a recess. Here, one of the two opposing surfaces of the second measurement device member 109 and the bottom surface in FIG. 3 (the surface facing the first measurement device member 108) function as the optical window portion 104.

  Next, the reagent holding portion 107 is formed on the bottom surface of the concave portion of the second measurement device member 109. For example, an aqueous solution of an antibody against human albumin, which is a reagent for optical measurement, is applied by dropping a predetermined amount onto the bottom surface of the concave portion of the second measurement device member 109 using a microsyringe or the like, and this is applied at room temperature to 30 The reagent can be supported in a dry state by allowing it to stand in an environment of about ° C. and evaporate the water.

The concentration of the antibody aqueous solution, the dropping amount, and the area of the dropping portion are, for example, a concentration of 8 mg / ml, the dropping amount of 25 μL, and the dropping portion area of 1 cm ² . An appropriate value can be selected as the concentration and amount of the aqueous solution containing the reagent to be applied in accordance with the required characteristics of the measurement device and the spatial limitation of the formation position on the second measurement device member 109. The position and area of the reagent holding part in the second measurement device member 109 are appropriately selected in consideration of the solubility of the reagent in the sample, the position of the optical window part 104, and the like.

  An antibody against human albumin can be obtained by a conventionally known method. For example, a rabbit antiserum immunized with human albumin is purified by protein A column chromatography, and then dialyzed using a dialysis tube to obtain an anti-human albumin antibody.

  As with the second measurement device member 109, the first measurement device member 108 can be obtained by molding using a mold. Alternatively, it can be produced by cutting a plate-like resin into a desired shape.

  Further, a pair of conductive portions 105c and 105d, electrodes 105e and 105f, and bonding lead portions 105a and 105b are disposed on the surface of the first measurement device member 108. For example, by placing an acrylic resin mask having a gap in the same shape as the pair of conductive portions 105c and 105d on the first measuring device member 108, sputtering the gold through the mask, and then removing the mask A pair of conductive portions 105c and 105d can be formed. Instead of sputtering, vapor deposition can be formed in the same procedure.

  The dimensions of the pair of conductive portions 105c and 105d are not particularly limited, but may be, for example, about 1 mm in width, about 45 mm in length, and about 5 μm in thickness, respectively. In order to define the size (length) of the electrodes 105e and 105f and the joint connection lead portions 105a and 105b, a cover 106 made of an insulating resin is stuck so that both ends of the conductive portions 105c and 105d are exposed. As the cover 106, for example, a film made of polyethylene terephthalate (PET) having a width of about 7 mm, a length of about 25 mm, and a thickness of about 0.1 mm and coated with an acrylic adhesive can be used. The cover 106 is arranged so that the lengths of the electrodes 105e and 105f are each 10 mm, for example, and the lengths of the joining lead portions 105a and 105b are each 10 mm, for example.

The material, area, thickness, shape, position, and the like of the conductive portion, the electrode, and the joint connection lead portion can be appropriately adjusted in view of required device characteristics.
The first measurement device member 108 and the second measurement device member 109 obtained as described above are joined together with the positional relationship indicated by the broken line shown in FIG. 3 to assemble the measurement device body. After applying an adhesive such as an epoxy resin to the joint portion of each member, the members are assembled and allowed to stand and dried by drying the joint portion. Alternatively, after joining the members, the joining portion may be welded by heat or ultrasonic waves using a commercially available welding machine.
As described above, the measuring device 1 of the present embodiment having the structure as shown in FIGS. 1 and 2 can be obtained.

(2) Measuring apparatus Next, the structure of the measuring apparatus 2 which can use the measuring device of this Embodiment suitably is demonstrated using FIGS. FIG. 4 is a perspective view showing the measuring device 2 in the present embodiment, FIG. 5 is a block diagram showing the configuration of the measuring device 2, and FIG. 6 is a schematic cross-sectional view for explaining the structure of the measuring device 2. FIG.

  As shown in FIG. 4, the measurement apparatus 2 includes a measurement device attachment unit 201 for detachably attaching the measurement device 1, a sample supply button 202 for starting supply of a sample to the measurement device 1, and a measurement device after measurement. 1 is provided with a sample discharge button 203 for discharging the sample in 1 to a paper cup, for example, and a liquid crystal display as a display unit 207 for displaying the measurement result.

  Further, as shown in FIG. 6, a measuring device attaching / detaching portion 211 is provided inside the measuring device attaching portion 201, and when the measuring device 1 is attached, the measuring device attaching / detaching portion is opened in the opening 101. Part 211 is inserted. At this time, a sealing ring made of a resin having elasticity such as fluorine resin such as Teflon (registered trademark) or isoprene rubber is provided around the measurement device attaching / detaching portion 211 so that air leakage does not occur in the joint portion. It is preferable to improve the adhesion between the device attaching / detaching portion 211 and the opening 101.

  As shown in FIG. 6, the measuring device 2 includes a motor 208 for moving a plunger 209 provided inside the cylinder 210 via a plunger joint 212. The motor 208, the plunger 209, and the cylinder 210 correspond to the suction mechanism 403 shown in FIG. 5 for sucking the sample liquid and air to the sample liquid holding unit 103 of the measurement device 1. An O-ring 213 is provided inside the cylinder 210 and airtightness is maintained between the cylinder 210 and the plunger 209.

  In addition, as shown in FIG. 5, a CPU 401 that is an arithmetic unit for detecting or quantifying a test substance contained in a sample based on the emitted light received by the light receiver 206 is provided inside the measurement device 2. In addition, a voltage is applied to the memory 402, which is a storage unit in which a calibration curve representing the relationship between the concentration of human albumin that is a test substance and the intensity of emitted light received by the light receiver 206 is stored, and the electrodes 105e, 105f of the measurement device 1. A voltage applying unit 408 for applying a voltage and an electric signal measuring unit 409 for measuring an electric signal obtained from the electrodes 105e and 105f are provided.

  As the light source 205 in this embodiment, a semiconductor laser that emits light having a wavelength of 650 nm is used. Instead of this, a light emitting diode (LED) or the like may be used. In this embodiment, the measurement by the immuno-ratio brazing method is applied, and the irradiation and light reception wavelength of 650 nm is selected. However, an appropriate value can be selected as the wavelength according to the measurement method and the measurement target. A photodiode is used as the light receiver 206 in this embodiment. Alternatively, a photo multimeter or the like may be used instead.

The suction mechanism 403 in the present embodiment is configured to operate the plunger 209 in the cylinder 210 by a motor 208 that is a linear step motor.
The step motor is a motor that rotates a specific rotation angle per inputted pulse signal, and the rotation angle can be determined by the number of pulses, so that an encoder for positioning is not required. That is, the operating distance of the plunger (piston) can be controlled by the number of input pulses. The rotational movement of the motor is converted into a linear movement using a gear mechanism and a linear mechanism combining a male screw and a female screw, thereby operating the plunger. The linear step motor incorporates a linear mechanism that combines male and female screws in the motor, and the plunger joint, which is a rod-shaped movable part, is configured to linearly move depending on the number of input pulses. Has been. For this reason, it suffices to connect the plunger directly to the plunger joint, and the configuration becomes simple.

(3) Measuring Method Next, a procedure for measuring a test substance in a sample using the measuring device 1 and the measuring apparatus 2 of the present embodiment will be described with reference to FIGS. 7 to 14 are schematic diagrams illustrating a procedure for measuring a test substance in a sample using the measurement device 1 and the measurement apparatus 2 of the present embodiment. An example using urine as a sample will be described below. In the present embodiment, the measuring device 1 and the measuring apparatus 2 are used so that the length directions of the measuring device 1 and the measuring apparatus 2 are substantially parallel to the vertical direction indicated by the arrow X in FIG. .

  FIG. 7 shows a state before the measuring device 1 is attached to the measuring apparatus 2. First, the user attaches the measuring device 1 to the measuring apparatus 2 in the direction of the arrow in the figure. At this time, the measuring device 1 is attached to the measuring device 2 through the measuring device mounting portion 201 so that the measuring device attaching / detaching portion 211 of the measuring device 2 and the opening 101 of the measuring device 1 are joined (FIG. 8). As a result, the joining lead portions 105a and 105b and the two terminals provided inside the measuring device mounting portion 201 are in contact with each other so as to be electrically connected to the pair of electrodes 105e and 105f of the measuring device 1.

  At this time, a measurement device insertion detection switch (not shown) composed of a micro switch provided in the measurement apparatus 2 is activated, and the CPU 401 functioning as a control unit detects the insertion of the measurement device 1 and the voltage application unit 408. Thus, a voltage (for example, a voltage at which the electrode 105e becomes +0.5 V compared to the electrode 105f) is applied between the pair of electrodes 105e and 105f of the measuring device 1.

  Next, the user immerses at least the supply port 102 of the measurement device 1 in the urine 301 discharged to the paper cup 3 (FIG. 9). When urine comes into contact with the pair of electrodes 105e and 105f, a current flows between the electrodes, so that the electrical signal measurement unit 409 detects a change in electrical signal resulting from the change, for example, a decrease in resistance value.

  Next, in this state, when the user presses the sample supply button 202, the CPU 401 operates the suction mechanism 403. Specifically, the motor 208 in the measuring apparatus 2 is driven, and the plunger 209 inside the cylinder 210 is pulled up via the plunger joint 212, whereby the sample liquid holding unit 103 is supplied from the supply port 102 of the measuring device 1. A predetermined amount (for example, 100 μL) of urine 301 is supplied into the inside (FIG. 10). By holding the plunger 209 in that position, urine is held in the sample liquid holding unit 103 and does not leak out from the supply port 102 or sucked into the cylinder 210. When the motor 208 stops and the suction operation by the suction mechanism 403 ends, the CPU 401 causes the display unit 207 to display a message notifying that the supply of air into the sample liquid holding unit 103 has been completed.

  The urine supplied into the sample liquid holding unit 103 starts to dissolve the anti-human albumin antibody, which is a dry reagent carried on the reagent holding unit 107, and at the same time, human albumin and anti-human albumin that are antigens in the urine The immune reaction with the antibody begins to progress.

  Next, the user pulls up the opening 102 from the urine 301 (FIG. 11). When the pair of electrodes 105e and 105f is separated from the urine, the resistance between the pair of electrodes 105e and 105f increases, so that no current flows. Therefore, since the electric signal measuring unit 409 detects again the change of the electric signal, for example, the change of the resistance value, the CPU 403 operates the suction mechanism 403 again with the detection, and the sample liquid holding unit 103 to which urine is supplied is applied. Supply air. Since the supply port 102 is provided between the tip of the pair of electrodes 105e and 105f and the opening 101, the supply port 102 can be reliably detected by detecting a change in the electrical signal between the pair of electrodes 105e and 105f. It is possible to detect that the urine has been lifted from the urine.

  Specifically, the motor 208 in the measuring apparatus 2 is driven, and the plunger 209 inside the cylinder 210 is further pulled up at a constant speed (for example, 1140 μL / s) via the plunger joint 212, whereby the measuring device 1 A predetermined amount (eg, 900 μL) of air is supplied from the supply port 102 into the sample solution holding unit 103 (FIG. 12). When air is supplied from the supply port 102, bubbles 302 are generated in the urine held in the sample liquid holding unit 103, whereby the urine and the reagent are agitated. When the motor 208 is stopped and the suction operation by the suction mechanism 403 is finished, the CPU 401 displays a message notifying that the supply of air into the sample liquid holding unit 103 is completed on the display unit 207, and at the time measuring unit 404. Start timing by a certain timer.

  In this way, by detecting that the supply port 102 has been pulled up from the urine 301, the urine 301 is mistakenly sucked into the sample solution holding unit 103 when air for stirring is supplied to the sample solution holding unit 103. Since it can prevent, the malfunction of stirring can be prevented. Further, since the air is automatically supplied to the sample liquid holding unit 103 by detecting that the supply port 102 is pulled up from the urine 301, the work of the user can be reduced.

Next, when the CPU 401 determines that a predetermined time (for example, 2 minutes) has elapsed from the completion of the supply of air into the sample holding unit 103 based on a signal from the time measuring unit 404, the CPU 401 causes the light source 205 to perform light irradiation. (FIG. 13).
Laser light emitted from the light source 205 is irradiated to the urine in the sample liquid holding unit 103 through the optical window 104, and the light scattered in the urine and emitted from the optical window 104 is received by the light receiver 206. The CPU 401 reads a calibration curve representing the relationship between the emitted light intensity and the human albumin concentration stored in the memory 402, and the CPU 401 converts the emitted light intensity received by the light receiver 206 into a human albumin concentration by referring to the calibration curve. To do. The obtained human albumin concentration is displayed on the display unit 207. By displaying human albumin on the display unit 207, the user knows that the measurement of human albumin concentration has been completed. Preferably, the obtained human albumin concentration is stored in the memory 402 together with the time measured by the time measuring unit 404.

  Next, the user moves the measuring device 2 or the paper cup 3 on which the measuring device 1 is mounted so that the supply port 102 of the measuring device 1 is positioned above the paper cup 3. In this state, when the user presses the sample discharge button 203, the motor 208 is driven, and the plunger 209 inside the cylinder 210 is pushed down via the plunger joint 212, so that the urine in the sample holding unit 105 is transferred to the paper cup. 3 is discharged (FIG. 14). The user drains and discards the urine 301 discharged into the paper cup 3 and then discards the paper cup 3. Further, the user extracts and discards the measuring device 1 in the direction indicated by the arrow.

  Furthermore, the obtained human albumin concentration can be recorded on a storage medium such as an SD card by the recording unit 405. By storing in a removable storage medium, the measurement result can be easily taken out from the measurement apparatus 2, so that the storage medium can be brought to an analysis specialist or mailed to request an analysis.

Furthermore, the obtained human albumin concentration can be transmitted outside the measuring apparatus 2 by the transmission unit 406. As a result, the measurement result can be transmitted to the analysis-related department or analysis-related contractor in the hospital and analyzed by the analysis-related department or analysis-related contractor, so that the time from measurement to analysis is shortened. be able to.
Furthermore, a receiving unit 407 is provided for receiving results analyzed in the analysis related department or analysis related business. Thereby, the analysis result can be quickly fed back to the user.

  In the above embodiment, as the suction mechanism 403, the device configuration in which the plunger 209 in the cylinder 210 is operated by the motor 208 which is a linear step motor is used, but the present invention is not limited to this. A step motor other than the linear type, a DC motor, or the like may be used.

  In the above embodiment, urine is supplied by the user pressing the sample supply button 202. However, the present invention is not limited to this, and the CPU 401 changes the electrical signal between the pair of electrodes 105e and 105f. When detecting that at least the supply port 102 of the measuring device 1 is immersed in urine, the CPU 401 may automatically operate the suction mechanism 403 to supply urine.

Moreover, in the said embodiment, although urine 301 was extract | collected in the paper cup 3, not only this but transportable containers, such as a plastic cup, a urine container provided in the toilet bowl, etc. may be used. .
In the above embodiment, a message notifying that the supply of urine and air into the sample solution holding unit 103 is completed is displayed on the display unit 207. Instead, a message such as a buzzer is used for notification. May be.

  According to the stirring method of the present invention, since a small and simple stirring mechanism can be used, it is useful in the field of analysis and inspection.

It is a perspective view which shows the structure of the measuring device which concerns on one embodiment of this invention. It is a schematic sectional drawing of the measuring device. It is a disassembled perspective view of the same measuring device. It is a perspective view which shows the structure of the measuring apparatus which concerns on one embodiment of this invention. It is a block diagram which shows the structure of the measuring device. It is sectional drawing which shows the structure of the measuring device. It is the schematic which shows 1 process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment. It is the schematic which shows the other process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment. It is the schematic which shows the other process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment. It is the schematic which shows the other process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment. It is the schematic which shows the other process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment. It is the schematic which shows the other process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment. It is the schematic which shows the other process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment. It is the schematic which shows the other process in the procedure which measures the test substance in a sample using the measuring device and measuring apparatus of the embodiment.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Measuring device 2 Measuring apparatus 3 Paper cup 101 Opening part 102 Supply port 103 Sample liquid holding | maintenance part 104 Optical window part 105a, 105b Joining lead part 105c, 105d Conductive part 105e, 105f Electrode 106 Cover 107 Reagent holding part 108 1st measuring device Member 109 Second measurement device member 201 Measurement device attachment portion 202 Sample supply button 203 Sample discharge button 205 Light source 206 Light receiver 207 Display portion 208 Motor 209 Plunger 210 Cylinder 211 Measurement device attaching / detaching portion 212 Plunger joint 213 O-ring 301 Urine 302 Bubble 401 CPU
402 Memory 403 Suction mechanism 404 Timekeeping unit 405 Recording unit 406 Transmitting unit 407 Receiving unit 408 Voltage applying unit 409 Electric signal measuring unit

Claims (3)

A substrate, a sample solution holding unit provided inside the substrate for holding a sample solution, a supply port communicating with the sample solution holding unit and supplying the sample solution and gas to the sample solution holding unit; An opening communicating with the sample solution holding unit, a reagent provided in the sample solution holding unit and specifically reacting with a test substance contained in the sample solution, and a pair of electrodes provided on the outer surface of the substrate A measuring device comprising:
A measuring device mounting portion for mounting the measuring device by being connected to the opening of the measuring device;
A suction means for sucking the gas in the sample liquid holding part through the opening of the measurement device attached to the measurement device attachment part;
A voltage application unit for applying a voltage between the pair of electrodes of the measurement device;
An electrical signal measuring unit for measuring electrical signals from the pair of electrodes;
Using a measuring device equipped with
(A) supplying the sample solution to the sample solution holding unit through the supply port;
(B) applying a voltage between the pair of electrodes;
(C) measuring an electrical signal from the pair of electrodes;
(D) detecting the supply port taken out of the sample liquid based on the change in the electrical signal;
(E) A method of stirring the sample liquid, including automatically supplying the gas to the sample liquid holding part through the supply port based on the detection in the step (D) in the sample liquid holding part. Before the step (A),
(F) applying a voltage between the pair of electrodes;
(G) measuring an electrical signal from the pair of electrodes;
(H) detecting that the supply port is immersed in the sample liquid based on the change in the electrical signal;
The sample liquid stirring method according to claim 1, further comprising: In the measuring device,
The sample solution stirring method according to claim 1, wherein the supply port is provided between a tip portion of the pair of electrodes and the opening.

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