JP2007304001A - Analyzer and analysis method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an analyzer and an analysis method requiring only a small labor or a little time necessary for analysis, capable of detecting a change of a sample in real time. <P>SOLUTION: This analyzer 1 is characterized by being equipped with an eluate route 5 along which eluate flows continuously, a holding means 13 for holding the sample in contact with the eluate in the middle of the eluate route 5, an addition means 19 for adding a reagent to the eluate on the furthermore upstream than the holding means 13 on the eluate route 5, and a detection means 15 for detecting a component included in the eluate on the furthermore downstream than the holding means 13 on the eluate route 5. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an analysis apparatus and an analysis method for analyzing hair, for example.

When developing a hair cosmetic or the like, it is necessary to evaluate the chemical reaction between the active ingredient contained in the hair cosmetic and the hair component, the physicochemical adsorption of the active ingredient on the hair surface, and the like.
As a conventional method for evaluating hair, there is a method in which hair itself is first dissolved or pulverized, a target component is taken out, and then the taken out component is analyzed. As another evaluation method, first, the hair is immersed in the treatment liquid to elute the target component, and then the treatment liquid is dried to obtain the target component, and the component is analyzed. There was a method of performing (refer patent document 1).
JP 2001-264323 A

  However, the conventional evaluation method has a problem that it requires a lot of labor and time because a sample must be prepared by the above-described procedure. In addition, when evaluating the reaction between the active ingredient of hair cosmetics and the hair, as described above, since the analysis can be performed only after preparing the sample over a long time, a long time has passed since the reaction occurred. There was a problem that the contents of the reaction could not be known without it.

  The present invention has been made in view of the above points, and an object of the present invention is to provide an analysis apparatus and an analysis method that can reduce labor and time required for analysis and can detect a change in a sample in real time.

(1) The invention of claim 1
An eluent path that is a path through which the eluent flows continuously; a holding means that holds the sample in contact with the eluent in the middle of the eluent path; and the holding means among the eluent paths An adding means for adding a reagent to the eluent upstream of the eluent, and a detecting means for detecting a component contained in the eluent downstream of the holding means in the eluent path. The gist of the analyzing apparatus is a feature.

  In the analyzer of the present invention, the reagent added by the adding means flows downstream in the eluent path together with the eluent, and reaches the sample held by the holding means. The component generated by the interaction between the reagent and the sample flows together with the eluent further downstream in the eluent path and reaches the detection means. And a detection means detects the said component.

  As described above, in the analyzer of the present invention, when the reagent is added by the reagent adding means, the substance generated by the interaction between the reagent and the sample is immediately detected by the detecting means. That is, the interaction between the reagent and the sample can be detected in real time.

  The analyzer of the present invention can easily analyze a sample simply by attaching the sample to the holding means. That is, unlike the conventional analysis method, it is not necessary to create a sample with much labor and time. Even when analyzing a plurality of samples in order, it is only necessary to replace the samples attached to the holding means.

The analyzer of the present invention is excellent in the reproducibility of detection data.
The analyzer of the present invention can be used for screening hair cosmetics (or active ingredients contained therein), for example.

  In other words, the hair as a sample is attached to the holding means, the hair cosmetic as a reagent is introduced while changing its kind and concentration, and the outflow component from the hair at that time is detected, so that the hair cosmetic The action can be grasped.

  Moreover, if the analyzer of this invention is used, the process with respect to hair can be evaluated. That is, hair treated under various conditions is prepared, each hair is attached as a sample, and a predetermined reagent is introduced. The treatment for hair can be evaluated by the type and amount of components that have flowed out of the hair.

Examples of the eluent include ultrapure water, pH buffer solution, organic solvent diluent, surfactant diluent and the like.
The sample includes a biological sample and an artifact. Examples of the biological sample include human hair, animal hair (eg, sheep, goat, etc.), cotton, hemp and other plant fibers, human and animal skin, nails and the like. Examples of human hair include untreated hair, hair color, hair subjected to chemical treatment such as bleaching, and hair subjected to physical treatment such as tension and compression. Examples of the artificial material include fiber, film or sheet made of synthetic resin or semi-synthetic resin, or artificial leather.

  Examples of the reagent include those that cause a chemical reaction with the sample and those that adsorb to the sample. Specifically, a surfactant, a pH adjuster, an oxidizing agent (for example, hydrogen peroxide), A reducing agent, an oil agent, a humectant, etc. are mentioned. Examples of the reagent when the sample is human hair include those that cause a chemical reaction with hair components (melanin, cuticle, cortex, various proteins, etc.) and those that adsorb on the hair surface. Can be mentioned.

  The detection means can be widely used as long as it can detect the reagent or a component generated by a chemical reaction between the reagent and the sample. For example, a mass spectrometer (MS), an electron spin resonance analyzer ( ESR), ultraviolet spectrometer (UV), nuclear magnetic resonance analyzer (NMR), fluorescent X-ray analyzer, light scattering analyzer and the like.

Examples of the component contained in the eluent include a substance generated by a chemical reaction between the reagent and the sample, a substance that has flowed out of the sample due to the action of the reagent, and a reagent that has not been adsorbed on the sample.
(2) The invention of claim 2
An eluent path that is a path through which the eluent flows continuously; a holding unit that holds the sample in contact with the eluent in the middle of the eluent path; A stimulus applying means for applying one or more stimuli selected from the group of sound waves and electricity, and a detecting means for detecting a component contained in the eluent downstream of the holding means in the eluent path. The gist of the analyzer is characterized by comprising.

  In the analyzer of the present invention, the sample that has been stimulated by the stimulus applying means generates some component due to the action caused by the stimulus. The component flows with the eluent downstream in the eluent path and reaches the detection means. The detecting means detects the component.

  As described above, in the analyzer of the present invention, when a stimulus is applied by the stimulus applying means, a substance generated by the stimulus is immediately detected by the detecting means. That is, the interaction between the stimulus and the sample can be detected in real time.

  The analyzer of the present invention can easily analyze a sample simply by attaching the sample to the holding means. That is, unlike the conventional analysis method, it is not necessary to create a sample with much labor and time. Even when analyzing a plurality of samples in order, it is only necessary to replace the samples attached to the holding means.

The analyzer of the present invention is excellent in the reproducibility of detection data.
Examples of the electromagnetic waves include ultraviolet rays, visible rays, and infrared rays. Examples of means for irradiating electromagnetic waves include a light irradiation device (for example, UVF-203S manufactured by Mitsunaga Electric Co., Ltd.).

The temperature of the sample when the heat is applied is preferably in the range of 30 to 120 ° C. Examples of the means for applying heat include a dryer and an infrared heater.
If the analyzer of this invention is used, the process with respect to hair can be evaluated. That is, hair treated under various conditions is prepared, each hair is attached as a sample, and a predetermined stimulus is applied. The treatment for hair can be evaluated by the type and amount of components that have flowed out of the hair.
(3) The invention of claim 3
The gist of the analyzer according to claim 2, further comprising addition means for adding a reagent to the eluent upstream of the holding means in the eluent path.

  Since the analyzer of the present invention also includes means for adding a reagent in addition to the stimulus applying means, for example, the reagent and the stimulus are added to the sample simultaneously or one after the other, and then the sample flows out from the sample. The component to be detected can be detected.

  Therefore, according to the present invention, for example, the synergistic effect of the reagent and the stimulus on the sample can be evaluated. In addition, in each of the cases where the reagent is introduced and not introduced, a component that flows out from the sample when a stimulus is applied to the sample is detected, and from this detection result, the influence of the reagent on the reactivity to the sample stimulus is detected. Can be evaluated.

Specifically, hair cosmetics (reagents) that prevent photooxidation (reaction) caused by ultraviolet irradiation (stimulation) are introduced into hair (sample), and then when the hair is irradiated with ultraviolet rays, The effect of preventing photooxidation of the hair cosmetic can be evaluated depending on whether or not the detection means detects a substance generated by photooxidation. Similarly, the effect of hair cosmetics (reagents) for preventing discoloration (reaction) caused by visible light irradiation (stimulation) of hair color hair (sample) can also be evaluated.
(4) The invention of claim 4
The gist of the analyzer according to any one of claims 1 to 3, wherein the sample is human or animal or plant fiber.

According to the present invention, human or animal and plant fibers can be analyzed.
Examples of the human or animal and plant fibers include human and animal hair, plant fibers, and the like.
(5) The invention of claim 5
The analysis according to claim 4, wherein the holding means immerses the human or animal or plant fiber in the eluent in a state in which a longitudinal direction thereof is substantially parallel to a flow direction of the eluent. The gist of the apparatus.

According to the present invention, the contact area between human or animal and plant fibers and the eluent can be increased. In addition, the eluent can be easily flowed.
(6) The invention of claim 6
A sample is brought into contact with the eluent flowing continuously, the reagent is added to the eluent upstream from the sample, and the components contained in the eluent downstream from the sample. The gist of this is an analysis method characterized by detecting.

(7) The invention of claim 7
A sample is brought into contact with a continuously flowing eluent, and one or more stimuli selected from the group consisting of electromagnetic waves, heat, ultrasonic waves, and electricity are applied to the sample, The gist is an analysis method characterized by detecting a component contained in the eluent downstream.
(8) The invention of claim 8
The gist of the analysis method according to claim 7, wherein a reagent is added to the eluent upstream of the sample while applying the stimulus.
(9) The invention of claim 9
The gist of the analysis method according to any one of claims 6 to 8, wherein the sample is human or animal or plant fiber.
(10) The invention of claim 10
The gist of the analysis method according to claim 9, wherein the human or animal or plant fiber is immersed in the eluent in a state in which a longitudinal direction thereof is substantially parallel to a flow direction of the eluent. .

The present invention will be described based on examples.

a) Overall Configuration of Analyzer 1 The overall configuration of the analyzer 1 will be described with reference to FIG. The analyzer 1 includes an eluent tank 3 that stores the eluent, a pipe 5 that is a path through which the eluent flows continuously, a pump 7 that sends the eluent from the eluent tank 3 to the pipe 5, and a middle of the pipe 5. 6-way switching valves 9 and 11 provided in the middle of the pipe 5 and downstream of the 6-way switching valves 9 and 11 with respect to the flow of the eluent, and the most downstream of the pipe 5 ESR15 provided in the.

  The eluent is ultrapure water that has been completely degassed. In the six-way switching valve 9, the upstream pipe 5 is connected to A2, the downstream pipe 5 is connected to A1, and A3 is connected to the drain. Therefore, when A1-A2 is connected in the 6-way switching valve 9, the eluent sent from the upstream flows further downstream in the pipe 5. When A2-A3 is connected, the eluent sent from the upstream flows to the drain and does not flow downstream in the pipe 5.

  In the six-way switching valve 11, the upstream pipe 5 is connected to B2, the downstream pipe 5 is connected to B3, and B1 and B4 are connected to both ends of the sample loop 17, respectively. . Accordingly, when B2-B3 is connected in the 6-way switching valve 11, the eluent sent from the upstream flows as it is further downstream in the pipe 5. When B1-B2 is connected and B3-B4 is connected, the eluent sent from the upstream flows through the sample loop 17 and then further downstream in the pipe 5. Sample loop 17 is a PEEK tube with a volume of 500 μL. The 6-way switching valve 11 is provided with an inlet 19 for introducing a reagent into the sample loop 17.

The ESR 15 is a flow injection ESR, and is TE-300 (model number) manufactured by JEOL. The ESR 15 includes an ESR flat cell 21. The ESR flat cell 21 is a flat cell LC11 (model number) manufactured by JEOL.
b) Structure of hair column Next, the structure of the hair column 13 and its periphery will be described with reference to FIG. The hair column 13 is composed of a glass tube 23 open on both sides, an inner joint 25, an outer joint 27, and an o-ring 29 made of silicon attached to both ends thereof.

  The dimensions of the glass tube 23 are an outer diameter of 4 mm, an inner diameter of 2 mm, and a length of 11 cm, and the material is quartz glass that easily transmits ultraviolet rays. In addition, the material of the glass tube 23 can be standard glass which is easy to transmit these electromagnetic waves, when irradiating electromagnetic waves other than ultraviolet rays to an internal sample.

  The inner joint 25 is a substantially cylindrical member made of Teflon (registered trademark), and has an external thread portion 31 on the outer peripheral surface thereof. The outer joint 27 is a substantially cylindrical Teflon (registered trademark) member, one of which is closed by a bottom surface 33, and has a female screw part 35 on the inner surface side. The outer joint 27 is connected to the pipe 5 through a screw hole 37 provided in the bottom surface 33.

When the o-ring 29 is attached between the outer joint 27 and the inner joint 25 and the male thread portion 31 of the inner joint 25 is tightened into the female thread portion 35 of the outer joint 27, the o-ring 29 is removed from both sides. By being pressed, it protrudes inward and adheres closely to the outer peripheral surface of the glass tube 23. As a result, the path from the upstream pipe 5 through the hair column 13 to the downstream pipe 5 can be sealed against the outside, and the inner joint 25 and the outer joint 27 are connected to the glass tube 23. It can be fixed against.
c) Analysis Method First, as shown in FIG. 2, the hair column 13 was filled with hair 39 as an analysis sample. At this time, the length of the hair 39 was aligned so as to be the same as the length of the glass tube 23 in the longitudinal direction. Then, the plurality of hairs 39 extended linearly were filled into the glass tube 39 so that the longitudinal direction thereof coincided with the longitudinal direction of the glass tube 39. The amount of hair 39 to be filled was 125.5 mg.

  When filling the hair 39 into the hair column 13, the hair 39 is filled into the glass tube 23 with the outer joint 27 and the inner joint 25 removed, and then again the outer joint 27 and the inner joint 25. And attached.

Next, while the 6-way switching valve 9 is in a state where A1-A2 is connected, and the 6-way switching valve 11 is in a state where B2-B3 is connected, the eluent is allowed to flow through the pipe 5. At this time, the eluent sequentially flows through a path of the pump 7, the 6-way switching valve 9, the 6-way switching valve 11, the hair column 13, and the ESR 15. At this time, the flow rate of the eluent was 1 ml / min. The measurement conditions for ESR15 were set as follows.
(Measurement conditions for ESR15)
Resonance frequency: 9.455 GHz
Resonance frequency output: 3 mW
Observed magnetic field region: Arbitrary magnetic field modulation amplitude intensity: 0.2 mT
Observation time constant: 0.03 sec
Next, reagent injection was performed on the analyzer 1. The reagent is a mixture of 50 mM 5,5 dimethyl-1-pyrroline-N-oxide (DMPO) aqueous solution and 35% hydrogen peroxide at a ratio of 9: 1. The final concentration is 45 mM DMPO and hydrogen peroxide. 3.5%. Moreover, the injection amount per one time of the reagent is 500 μL.

The reagent injection method was as follows. The reagent is introduced into the sample loop 17 from the introduction port 19 using the syringe 41. At this time, since the state of the six-way switching valve 11 is a state where B2-B3 is connected, the sample loop 17 is disconnected from the path of the eluent. Next, the state of the 6-way switching valve 11 is switched to a state where B1-B2 is connected and B3-B4 is connected. Then, since the eluent passes through the sample loop 17, the reagent introduced into the sample loop 17 flows downstream together with the eluent. The reagent that has flowed downstream reaches the hair column 13. In the hair column 13, the reaction between the reagent and the hair 39 occurs, and the reaction product reaches the ESR 15 together with the eluent. The above sample introduction was performed three times in total every predetermined time.
d) Analysis Results Next, the detection data of ESR15 at the time of reagent introduction will be described. FIG. 3 is a measurement chart of ESR15 when black hair is used as the hair 39, and three portions surrounded by a circle are portions corresponding to the first to third sample injections, respectively. FIG. 4 is an enlarged view of a portion corresponding to the second sample introduction in FIG. In addition, the signal with a large repetition in FIG.3 and FIG.4 is a signal of manganese of a reference material. As apparent from FIGS. 3 and 4, detection peaks appear with good reproducibility corresponding to sample injection.

  The detection peaks appearing in the charts of FIG. 3 and FIG. 4 show that hydrogen peroxide contained in the reagent and melanin in the hair undergo an electronic reaction to generate OH radicals, which are then converted into DMPO in the reagents. Is detected by trapping and gaining life.

  FIG. 5 is a chart of the ESR 15 when white hair is used as the hair 39. FIG. 6 is an enlarged view of the portion corresponding to the second sample introduction in FIG. As is clear from FIGS. 5 and 6, no peak corresponding to the sample injection appears. This is because OH radicals were hardly generated because the amount of melanin in gray hair was small.

That is, OH radicals generated by the reaction between hydrogen peroxide in the reagent and melanin in the hair could be detected by the above analysis method. If this analysis method is used, the amount of OH radicals corresponding to the hydrogen peroxide concentration can be quantified in real time.
e) Comparative Example First, the hair was pulverized, and then the powdered hair was mixed with 35% hydrogen peroxide and 50 mM DMPO to prepare a powder mixed sample. Here, the amounts of hair powder, 35% hydrogen peroxide solution, and 50 mM DMPO were 30 mg, 100 μL, and 900 μL, respectively. This powder mixed sample was directly introduced into ESR 15 three times at predetermined time intervals. A chart of the ESR 15 at that time is shown in FIG. The three peaks in FIG. 7 are detection peaks corresponding to the first to third sample introductions, respectively. As shown in FIG. 7, the OH radical detection peak appeared, but the peak size was not reproducible.
f) Effects of the analyzer 1 and the analysis method
(i) The analyzer 1 can simply analyze the hair 39 by simply putting the hair 39 into the hair column 13. Even when analyzing a plurality of samples (hairs 39), it is only necessary to replace the hair in the hair column 13.
(ii) When the reagent is introduced into the analyzer 1, the substance generated by the reaction between the reagent and the hair 39 is immediately detected by the ESR 15. That is, the interaction between the reagent and the hair can be detected in real time.
(iii) The analyzer 1 is excellent in the reproducibility of detection data.
(iv) If the analyzer 1 is used, it is possible to easily screen hair cosmetics (or active ingredients contained therein). That is, the action of the hair cosmetic can be grasped by introducing the hair cosmetic as a reagent while changing its type and concentration and detecting the outflow component from the hair at that time.

  Examples of the hair cosmetics include those containing amino acids as active ingredients. By introducing an amino acid as a reagent while changing its type and concentration, and detecting the amount of amino acid passing through the hair column 13 at that time, it is possible to compare whether the amino acid is easily adsorbed to the hair. That is, the smaller the amount of amino acid detected, the easier it is to adsorb to the hair.

Moreover, there are various shampoos as the hair cosmetics. The hair column 13 is filled with color-treated hair, various shampoos are introduced as reagents, and the dye flowing out of the hair column 13 is detected at that time, so that each shampoo flows out which dye and how much. It is possible to determine whether it is easy to do.
(v) If the analyzer 1 is used, the treatment for hair can be evaluated. That is, hair treated under various conditions is prepared, each hair is filled in the hair column 13, and a predetermined reagent is introduced. The treatment for hair can be evaluated by the type and amount of components that have flowed out of the hair.

  For example, in general, various contents flow out from the hair after the bleaching treatment, but it is possible to evaluate how much the outflow of the contents can be suppressed by the treatment before or after the bleaching. Specifically, hair A that has undergone pretreatment A, hair B that has undergone pretreatment B, hair C that has undergone pretreatment C, etc. are prepared, and each hair is subjected to bleach treatment under the same conditions. . Then, each hair is filled in the hair column 13 and a predetermined reagent is introduced. The effect of the treatment before bleaching (less content outflow) can be evaluated by the type and amount of the content flowing out from the hair.

  Further, hair subjected to bleaching treatment under the same conditions is classified as hair D, hair E, hair F, etc., hair D is subjected to post-treatment D, hair E is subjected to post-treatment E, hair F Is subjected to post-processing F, and so on. Then, each hair is filled in the hair column 13 and a predetermined reagent is introduced. The effect of the treatment after bleaching (less content outflow) can be evaluated by the type and amount of the content outflowing from the hair.

a) Configuration of Analyzer 1 The configuration of the analyzer 1 in the second embodiment is basically the same as that of the first embodiment. However, as shown in FIG. 8, instead of the ESR 15, a UV detector 43, An RI detector 45 and an MS detector 47 were connected in series.

b) Analysis method First, the hair column 13 was filled with untreated hair. The filling method is the same as in Example 1. As in Example 1, a high concentration aqueous solution of sodium lauryl sulfate (SLS) was introduced as a sample while the eluent was flowing through the pipe 5. Here, the concentration of the SLS high-concentration aqueous solution is 25%, and the introduction amount is 500 μL.

At this time, the UV detection device 43, the RI detection device 45, and the MS detection device 47 detected the lipids and proteins that were generated by the reaction between the reagent and the hair and flowed out of the hair.
Next, the hair column 13 was replaced with hair color hair, and the same analysis was performed. At this time, the UV detection device 43, the RI detection device 45, and the MS detection device 47 detected the melanin pigment, the dye, lipid, and protein contained in the hair color.

  Next, the hair filled in the hair column 13 was replaced with bleached hair, and the same analysis was performed. At this time, the UV detection device 43, the RI detection device 45, and the MS detection device 47 detected melanin pigments, lipids, and proteins.

a) Configuration of Analyzer 1 The configuration was the same as in Example 2.
b) Analysis method First, the hair column 13 was filled with untreated hair. The filling method is the same as in Example 1. In the same manner as in Example 1, the hair column 13 was irradiated with ultraviolet rays while the eluent was flowing through the pipe 5. In addition, since the material of the glass tube 23 which comprises the hair column 13 is quartz glass as mentioned above, an ultraviolet-ray permeate | transmits the glass tube 23 and reaches | attains hair.

  At this time, the UV detection device 43, the RI detection device 45, and the MS detection device 47 detected lipid, protein, and melanin pigment that flowed out of the hair. The lipids, proteins, and melanin pigments flow out due to the fact that the keratin covalent bond in the hair is cut by ultraviolet rays.

  Next, the same analysis was performed for the case where the hair to be filled in the hair column 13 was a hair-colored hair or a bleached hair. In these cases, lipids, proteins, and melanin pigments that flowed out of the hair were detected.

c) Effects of Analyzing Device 1 and Analyzing Method If the analyzing device 1 is used, it is possible to easily evaluate the effect of preventing hair damage caused by ultraviolet rays, which the hair cosmetic (or active ingredient contained therein) has. In other words, hair prepared with various types of hair cosmetics is prepared as a sample, and the effect of hair cosmetics depends on the type and amount of detection components (those generated by the action of ultraviolet rays) when each sample is used. Can be evaluated.

a) Configuration of Analyzer 1 The configuration was the same as in Example 2.
b) Analysis method First, the hair column 13 was filled with untreated hair. The filling method is the same as in Example 1. In the same manner as in Example 1, the hair column 13 was heated to 60 to 80 ° C. using a drier while the eluent was flowing through the pipe 5.

  At this time, the UV detection device 43, the RI detection device 45, and the MS detection device 47 detected lipids and proteins that flowed out of the hair. The lipid is melted and eluted by heat, and the protein flows out by protein denaturation.

  Next, the same analysis was performed for the case where the hair to be filled in the hair column 13 was a hair-colored hair or a bleached hair. In these cases, lipids and proteins that flowed out of the hair were detected. Furthermore, when the hair was hair color hair, the dye was also detected.

c) Effects of Analyzing Device 1 and Analyzing Method If the analyzing device 1 is used, it is possible to easily evaluate the effect of preventing hair damage caused by heat that the hair cosmetic (or active ingredient contained therein) has. In other words, hair prepared by applying various types of hair cosmetics is prepared as a sample, and the effect of hair cosmetics depends on the type and amount of detection components (those generated by the action of heat) when using each sample. Can be evaluated.

Needless to say, the present invention is not limited to the above-described embodiments, and can be implemented in various modes without departing from the scope of the present invention.
For example, instead of filling hair into the hair column 13 as it is, separating the hair into components such as cuticle, cortex, melanin, etc., and filling the hair column 13 with each component alone or by mixing several components. Can do.

  Moreover, the state of the hair when filling the hair column 13 may be cut short or powdered. When powdered, it is preferable to fill the hair column 13 with an inert component such as sea sand together with the hair to make the particle diameter coarse. By doing so, the eluent can easily pass through the hair column 13, and the differential pressure in the hair column 13 can be reduced.

  In Examples 3 and 4, instead of, or together with ultraviolet irradiation or heating, the hair in the hair column 13 is irradiated with ultrasonic waves or an electric current is applied to detect outflow components from the hair. May be.

  The sample filled in the hair column 13 may be animal hair (eg, sheep, goat, etc.) instead of human hair. Moreover, vegetable fiber, such as cotton and hemp, may be sufficient. Furthermore, it may be human or animal skin, nails or the like. The sample filled in the hair column 13 may be an artifact. Examples of the artificial material include fiber, film or sheet made of synthetic resin or semi-synthetic resin, or artificial leather.

  In Examples 3 to 4, a reagent may be introduced together with ultraviolet irradiation and heating. In this case, the synergistic effect of the reagent and ultraviolet irradiation (or heating) on the hair can be evaluated. Alternatively, in each case where the reagent is introduced and not introduced, a component that flows out from the hair when stimulation is applied to the hair is detected, and from this detection result, the influence of the reagent on the reactivity to the hair stimulation is detected. Can be evaluated.

  Specifically, hair cosmetics that prevent photooxidation due to ultraviolet irradiation have been introduced as a reagent, and then, how much a substance generated by photooxidation is detected when the hair is irradiated with ultraviolet rays, The photooxidation preventing effect of the hair cosmetic can be evaluated. Similarly, the effect of hair cosmetics that prevent discoloration of hair-colored hair due to visible light irradiation can also be evaluated.

2 is an explanatory diagram illustrating a configuration of an analysis apparatus 1. FIG. 3 is an explanatory diagram illustrating a configuration of a hair column 13. FIG. It is a measurement chart of ESR15. It is a measurement chart of ESR15. It is a measurement chart of ESR15. It is a measurement chart of ESR15. It is a measurement chart of ESR15. 2 is an explanatory diagram illustrating a configuration of an analysis apparatus 1. FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Analytical apparatus 3 ... Eluent tank 5 ... Piping 7 ... Pump 9, 11 ... 6 way switching valve 13 ... Hair column 15 ... ESR
17 ... Sample loop 19 ... Inlet 23 ... Glass tube 25 ... Inner joint 27 ... Outer joint 29 ... O-ring

Claims (10)

An eluent path that is a path through which the eluent flows continuously;
A holding means for holding the sample in contact with the eluent in the middle of the eluent path;
An adding means for adding a reagent to the eluent upstream of the holding means in the eluent path;
Detecting means for detecting a component contained in the eluent downstream of the holding means in the eluent path;
An analysis apparatus comprising: An eluent path that is a path through which the eluent flows continuously;
A holding means for holding the sample in contact with the eluent in the middle of the eluent path;
A stimulus applying means for applying one or more stimuli selected from the group consisting of electromagnetic waves, heat, ultrasonic waves and electricity to the sample;
Detecting means for detecting a component contained in the eluent downstream of the holding means in the eluent path;
An analysis apparatus comprising:   The analyzer according to claim 2, further comprising an adding unit that adds a reagent to the eluent upstream of the holding unit in the eluent path.   The analyzer according to claim 1, wherein the sample is human or animal or plant fiber.   The analysis according to claim 4, wherein the holding means immerses the human or animal or plant fiber in the eluent in a state in which a longitudinal direction thereof is substantially parallel to a flow direction of the eluent. apparatus. The sample is brought into contact with the continuously flowing eluent in the middle of the flow,
Add a reagent to the eluent upstream of the sample,
An analysis method, wherein a component contained in the eluent is detected downstream of the sample. The sample is brought into contact with the continuously flowing eluent in the middle of the flow,
Applying one or more stimuli selected from the group of electromagnetic waves, heat, ultrasound, electricity to the sample;
An analysis method, wherein a component contained in the eluent is detected downstream of the sample.   The analysis method according to claim 7, wherein a reagent is added to the eluent upstream of the sample while applying the stimulus.   The analysis method according to claim 6, wherein the sample is human or animal or plant fiber.   The analysis method according to claim 9, wherein the human or animal or plant fiber is immersed in the eluent in a state in which a longitudinal direction thereof is substantially parallel to a flow direction of the eluent.