JP2007297357A - Anti-microbial incense having wide anti-microbial spectrum - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an anti-microbial incense which has a wide anti-microbial spectrum, can daily be used to prevent opportunistic infections and the like, can especially exhibit wide microbial actions against bacteria and fungi causing food poisoning, purulent skin diseases, opportunistic infections, and the like, and can widely prevent infectious diseases caused by the bacteria and the fungi. <P>SOLUTION: This anti-microbial incense whose compounded anti-microbial ingredient is evaporated by combustion and diffused in an indoor space to exhibit an anti-microbial effect is characterized by compounding ATRACTYLODIS LANCEAE RHIZOMA as the anti-microbial ingredient. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an antibacterial incense that vaporizes an antibacterial component formulated by burning and exerts an antibacterial effect by the vaporized antibacterial component diffusing in an indoor space, and in particular, a bacterium that causes food poisoning, cutaneous suppuration disease, opportunistic infection, etc. The present invention relates to an antibacterial incense having a broad antibacterial spectrum that exerts a wide antibacterial action against fungi, and more specifically, an antibacterial incense, antibacterial incense and antibacterial incense burned directly by burning. It also includes antibacterial incense burned by direct fire of stick type, cone type, coil type and chip type.

  Traditionally, incense, represented by incense, has been used to create a strict atmosphere at religious events, to direct the scent to Buddha, or to enjoy the scent in the room. As incense for purposes other than these, mosquito-repellent incense using an insecticidal component of pesticide chrysanthemum and low smoke incense incense have been known for a long time (for example, see Patent Document 1).

  By the way, it is well known that there are a wide variety of bacteria and fungi around our lives, and even among those who do not cause infections in healthy people, they are resistant to children and the elderly. A large number of causative bacteria that cause opportunistic infections or the like caused by these bacteria in low-risk people have been confirmed. Especially in the medical field, nosocomial infections that infect patients with various basic diseases and patients who use immunosuppressants in organ transplantation, etc. are a serious problem related to human life. It is an urgent task to take measures to suppress certain causative bacteria.

  Against this background, in recent years, there have been many patent applications related to antibacterial incense, that is, incense and incense imparted with an antibacterial effect (see, for example, Patent Documents 2 to 3). For example, Patent Document 2 discloses an incense in which an antibacterial agent having a transpiration property such as hinokitiol is added. Moreover, in patent document 3, the incense which mixed and mixed the charcoal kiln wood vinegar liquid extract | collected from the smoke of charcoal in the raw material of an incense is disclosed.

  However, in any of Patent Documents 2 to 3, although the antibacterial effect of the incense stick or incense is enshrined, the specific antibacterial effect is not clearly specified for which bacteria, Furthermore, no experimental data supporting the effect is disclosed. For example, in Patent Document 2, the excellent antibacterial action of hinokitiol has been scientifically proved, and it is mixed with incense smoke and diffuses in the room. It is only to the extent that it can be understood even without it.

That is, in the background described above, there are attempts to make the room hygienic by adding antibacterial agents to incense sticks and incense, but the effect is vague and not always scientifically proven. It wasn't something.
Japanese Patent Application Laid-Open No. 07-309736 Japanese Patent Laid-Open No. 10-287539 Japanese Patent Laid-Open No. 11-012151

The problem to be solved by the present invention is to have a broad antibacterial action against antibacterial incense that can prevent opportunistic infections, etc. by daily use, especially bacteria and fungi that cause food poisoning, cutaneous suppuration diseases, opportunistic infections, etc. It is to provide an antibacterial odor with a wide antibacterial spectrum that can be exerted and widely prevent infectious diseases caused by these bacteria.
And living spaces and living spaces where children with low resistance and the elderly can live with peace of mind, patients with various basic diseases, patients using immunosuppressants in organ transplants, etc., and these It is to provide a medical environment where medical staff supporting patients and the like can concentrate on treatment with peace of mind.

  In order to solve the above problems, the inventor has independently developed a test method for confirming the antibacterial effect against causative bacteria that develop opportunistic infections, etc., and using the test method, various crude drugs and essential oils have been developed. Many steady experiments were conducted to confirm the antibacterial effect of the blended incense. And it was found that remarkable antibacterial activity against the causative bacteria was recognized especially in coconut (sojutsu) and clove (Choji), and in hiba oil, which is an essential oil, and each combination of strawberry, clove and hiba oil We prepared 30 kinds of incense with varying amounts and accumulated many experiments to quantify each antibacterial effect.

  The test bacteria to confirm the antibacterial activity of incense include a total of nine types of bacteria and fungi that are the causative agents causing food poisoning, cutaneous suppuration diseases, opportunistic infections, and more specifically: (1) Acinetobacter calcoaceticus ATCC19606, (2) Enterobacter aerogenes ATCC23355, (3) Escherichia coli ATCC25922, (4) Enterococcus faecalis ATCC33186, (5) Enterococcus faecalis ATCC19433, (6) Staphylococcus aureus ATCC29213, (7) Staphylococcus aureusATCC25923, C1 albicans ATCC10261 was adopted. The above bacteria (1) to (3) are Gram-positive bacteria represented by E. coli, (4) to (7) are bacteria classified as Gram-negative bacteria represented by Staphylococcus aureus, (8) to (9) are two types of Candida fungi that are fungi.

  The reason why these bacteria were selected as test bacteria is that these bacteria, etc. are resident in our daily lives and are familiar to our lives. This is because it has great technical significance. Moreover, it is because the technical significance which confirms the antibacterial activity of an incense is large. As a result of the above numerous experiments, the technical knowledge obtained by the present inventor is as follows.

(A) Soju is different from clove and hiba oil and is not excellent in fragrance, and is generally a crude drug that is unlikely to be blended in incense as a fragrance. However, the incense blended with cocoon as an antibacterial component is prominent against the bacteria of (2) to (3) and (6) to (9) among the above-mentioned test bacteria as compared to the incense that does not contain any crude drugs or essential oils. Exhibit antibacterial activity.

(B) In addition, the incense blended with persimmon exhibits a particularly remarkable antibacterial activity even when compared with incenses that do not contain any crude drugs or essential oils against the above-mentioned bacteria (1), (4), (5). No, but exhibits a certain level of antibacterial activity and generally exhibits a broad antibacterial spectrum of (1) to (9).

(C) Clove is a herbal medicine that is excellent in fragrance and has been conventionally blended into incense as a fragrance, and is a herbal medicine that has been used as an antiseptic sterilizing effect in addition to aroma (for example, As described above, the effect is vague, and the antibacterial effect against specific bacteria as shown in the above (1) to (9) is a herbal medicine that has not been scientifically proven. . Therefore, when the antibacterial effect is tested according to the above test method, the incense blended with clove as an antibacterial component is compared with the incense that does not contain any herbal medicine or essential oil, among the test bacteria (2) to (3), and ( 6) It exhibits remarkable antibacterial activity against the bacteria of (9).

(D) In addition, the incense blended with clove shows a particularly remarkable antibacterial activity compared to the incense that does not contain any crude drugs or essential oils against the above-mentioned bacteria (1), (4), (5). No, but exhibits a certain level of antibacterial activity and generally exhibits a broad antibacterial spectrum of (1) to (9).

(E) Hiba oil is an essential oil obtained by steam-distilling Hiba strains, roots or branches and leaves of the cypress family Asunaro, and hinokitiol, one of the essential oil components, exhibits antibacterial properties (for example, It is an essential oil that has been used as a perfume for soaps, toothpastes, and toiletry products. However, as described above, its effect is vague, and it is specific as shown in the above (1) to (9) Antibacterial effects against common fungi are essential oils that have not been scientifically proven. Therefore, when the antibacterial effect is tested according to the above test method, the incense containing Hiba oil as an antibacterial component is compared to the incense that does not contain any herbal medicine or essential oil, among the above test bacteria (2) to (3), and ( 6) It exhibits remarkable antibacterial activity against the bacteria of (9).

(F) Moreover, the fragrance | flavor which mix | blended hiba oil shows especially remarkable antimicrobial activity compared with the fragrance | flavor which does not contain a crude drug or an essential oil with respect to the microbe of said (1), (4), (5). No, but exhibits a certain level of antibacterial activity and generally exhibits a broad antibacterial spectrum of (1) to (9).

(G) An incense containing cocoon, clove and hiba oil alone as an antibacterial component has remarkable antibacterial activity against the bacteria of (2) to (3) and (6) to (9) among the above test bacteria In addition, as described above, the broad antibacterial spectrum of (1) to (9) is as described above, but the incense containing two kinds of persimmon and hiba oil is the above-mentioned (5), (7) and (8 2) Synergistic activity by mixing two kinds of bacteria.

(H) Furthermore, for the incense blended with three kinds of persimmon, clove and hiba oil, it shows synergistic activity by mixing three kinds against the bacteria of (5), (7), (8) and (9). thing.

(I) However, if it exceeds an appropriate blending amount, it becomes impossible to enjoy the fragrance, which is the original function of the incense. Specifically, if the mass of the cocoon exceeds 35% by mass, the unique scent of the cocoon becomes too strong to be suitable for indoor use. For clove, if it exceeds 35% by mass, the scent becomes too strong to be suitable for indoor use. Furthermore, if Hiba oil exceeds 20% by mass, the scent becomes too strong and it becomes difficult to mold.

  Based on the above findings, the present inventor will exert a broad antibacterial action against bacteria and fungi causing food poisoning, cutaneous suppuration disease, opportunistic infections, etc., and widely prevent infections caused by these bacteria I came up with an antibacterial incense with a broad antibacterial spectrum. The gist is as follows.

(1) An antibacterial spectrum characterized by the addition of sputum as an antibacterial ingredient in an antibacterial incense that exhibits an antibacterial effect by vaporizing the antibacterial ingredient blended by burning and diffusing the vaporized antibacterial ingredient in the indoor space Wide antibacterial incense.

(2) The antibacterial fragrance having a broad antibacterial spectrum as described in (1) above, further comprising Hiba oil as an antibacterial component.

(3) The antibacterial fragrance having a broad antibacterial spectrum according to (2), wherein clove is further blended as an antibacterial component.

(1) The antibacterial scent according to the present invention exhibits a broad antibacterial action against bacteria and fungi that are permanently present in general life and cause food poisoning, cutaneous suppuration diseases, opportunistic infections, etc. By using the antibacterial scent according to the above, various infectious diseases caused by these bacteria can be widely prevented. For example, when the antibacterial incense according to the present invention is used in the home, it can be used not only in the Buddha, but also in any living space / living space such as the living room, bedroom, kitchen, washroom, entrance, etc. Propagation of the causative bacteria can be easily suppressed, and even children and elderly people with low resistance can spend peace of mind without worrying about opportunistic infections.

(2) In addition, the antibacterial action of the antibacterial scent according to the present invention is exhibited when the antibacterial component blended in the scent is vaporized and diffuses in the indoor space. Or by burning for a time according to the space volume, not only in the home, which is a representative of the living space and living space described above, but also in a wide indoor space such as a school, office, various public facilities, etc. Can also exhibit the above-mentioned effects. In particular, when the antibacterial incense according to the present invention is used in an indoor space where a large number of people gather or an indoor space where food is handled and hygiene management is required, it can be expected that not only opportunistic infections but also food poisoning can be prevented. It is not difficult to imagine that is extremely enormous.

(3) Furthermore, in the hospital where patients with various basic diseases and patients using immunosuppressants for organ transplantation are hospitalized, the problem of nosocomial infection is becoming serious. Antibacterial incense exerts a broad antibacterial action against bacteria and fungi that cause opportunistic infections, etc., so it is very effective in preventing nosocomial infections and other infections. It can be expected that he can concentrate on treatment with peace of mind.

(4) That is, the antibacterial incense according to the present invention vaporizes the antibacterial component blended in the incense, and exerts a wide antibacterial action by diffusing the indoor space, food poisoning, skin suppuration disease, It is no exaggeration to say that it can be used in any space where there are bacteria and fungi that cause opportunistic infections, and its industrial applicability is extremely large. Further, the technical significance of scientifically demonstrating the antibacterial effect of a specific antibacterial fragrance containing a predetermined antibacterial component against a specific bacterium is extremely large.

Hereinafter, the best mode for carrying out the present invention will be described.
As described above, the antibacterial scent according to the present invention uniquely develops a test method for confirming the antibacterial effect against the causative bacteria causing the opportunistic infection etc., and uses the established test method. As a result of a lot of steady work to confirm the antibacterial effect of incense blended with various herbal medicines and essential oils, among herbal medicines, especially for soju and clove, and Hiba oil, which is an essential oil Is a completed invention based on the finding that remarkable antibacterial activity against the causative bacteria is recognized. Therefore, first, a test method for confirming the antibacterial effect against causative bacteria for causative bacteria that develop opportunistic infections, etc., and then experimental data derived by the test methods will be described.

(1) Acinetobacter calcoaceticus ATCC 19606:
Acinetobacter is a gram-positive bacterium that is widely distributed in natural soil, water, vegetables, and the like and can be a causative bacterium such as sepsis and pneumonia in immunocompromised patients and IV catheter patients.

(2) Enterobacter aerogenes ATCC 23355:
Enterobacter is a bacterium that is permanently present in the intestinal tract of humans and animals, and is also distributed in sewage, rivers, soil, food, etc., and causes septicemia, shock, pneumonia, suppuration associated with surgical wounds It is a fungus.

(3) Escherichia coli ATCC 25922:
Escherichia coli is a gram-positive bacterium that is a tumor bacterium that constitutes the intestinal flora and is generally said to be a causative bacterium of non-pathogenic opportunistic infections.

(4) Enterococcus faecalis ATCC 33186
(5) Enterococcus faecalis ATCC 19433:
Enterococcus is a gram-negative bacterium that is involved in various infections such as urinary tract infections, sepsis, and infections caused by trauma.

(6) Staphylococcus aureus ATCC 29213
(7) Staphylococcus aureus ATCC 25923:
Staphylococcus aureus is a gram-negative bacterium that has problems with wound infection and MRSA during surgery.

(8) Candida albicans ATCC10231
(9) Candida albicans ATCC10261:
Candida is a fungus that is present in the oral cavity, intestinal tract and the like in 20 to 40% of healthy people, and can overgrow Candida and cause enteritis.

  Next, although the test method for confirming the antibacterial effect with respect to the said causative microbe is demonstrated, the test method with respect to the bacteria of said (1)-(7) and the test method with respect to the fungus of (8)-(9) differ. Therefore, the test method for bacteria will be explained first.

The antibacterial test for each of the above bacteria (1) to (7) was performed as follows. First, each bacterium was cultured in a normal agar medium at 37 ° C. for 24 hours, and 1 platinum loop of each cultivated bacteria was cultured in 2 ml of meat extract broth for 24 hours.
Next, 5 ml of sterilized physiological saline was collected in a sterilized test tube A, and 5 μl of each bacterium cultured in the bouillon was added thereto and shaken. Then, 5 ml of sterilized physiological saline was collected in another sterilized test tube B, and 5 μl of sterilized test tube A was added thereto and shaken. Further, 5 ml of sterilized physiological saline was collected in another sterilized test tube C, and 5 μl of sterilized test tube B was added thereto and shaken.

  Next, 1 ml of the sterilized test tube C was dropped onto 2 sheets of Sani-Takun (registered trademark), a microorganism detection medium sheet for general viable bacteria manufactured by Chisso Corporation, and left in a cleave bench for 5 minutes. One sheet was fixed to the inner side surface of a 14.4 liter plastic container as a test sheet, and the container was sealed with a test incense, and the remaining sheets were used as controls. After 1 hour, the test sheet fixed on the inner side surface of the container was taken out of the container and cultured for 36 hours in a 37 ° C. incubator together with the control.

Finally, the number of colonies on each sheet was counted, and the inhibition rate was obtained by the following formula.
Inhibition rate (%) = (1−number of colonies in test sheet / number of colonies in control) × 100

The antibacterial test for each fungus of the above (8) to (9) was performed as follows. First, each fungus was cultured in a YPD agar medium at 37 ° C. for 24 hours, and one platinum loop of each cultured fungus was cultured in 2 ml of YPD for 24 hours.
Next, 5 ml of sterilized physiological saline was collected in a sterilized test tube D, and 5 μl of each bacterium cultured in YPD was added thereto and shaken. Then, 5 ml of sterilized physiological saline was collected in another sterilized test tube E, and 5 μl of the sterilized test tube D was added thereto and shaken.

  Subsequently, 1 ml of the sterilized test tube E was dropped on two sheets of fungus microorganism detection medium sheet Sani Tai-kun (registered trademark) manufactured by Chisso Corporation, and left in a cleave bench for 5 minutes. One sheet was fixed to the inner side surface of a 14.4 liter plastic container as a test sheet, and the container was sealed with a test incense, and the remaining sheets were used as controls. After 1 hour, the test sheet fixed on the inner side surface of the container was taken out of the container and cultured for 36 hours in a 37 ° C. incubator together with the control. Finally, the number of colonies on each sheet was counted, and the inhibition rate was obtained by the previous formula.

  In addition, as test incense burned in a plastic container having a volume of 14.4 liters, incense sticks having lengths of 3, 6, 9, and 15 cm were used for both the antibacterial test for bacteria and the antibacterial test for fungi.

Next, experimental data derived by the above test method will be described.
FIG. 1 is a graph showing the antibacterial effect of the antibacterial incense according to the present invention in which koji is blended as an antibacterial component, and remarkable antibacterial activity was recognized as compared with the incense containing no herbal medicine or essential oil (comparative example). It is the graph which showed the suppression rate with respect to the microbe of said (2)-(3) and (6)-(9). The suppression rate of incense containing 5% by weight of candy is indicated by white triangles (Δ), and the suppression rate of incense containing 10% by weight of strawberry is indicated by white circles (（).
The incense that does not contain any herbal medicine or essential oil, which is a comparative example, is a mixture of 40% by mass of coconut shell charcoal powder, 25% by mass of binder containing tab bark powder, and 35% by mass of alumina oxide powder. Alternatively, it is an incense stick that is formed by kneading hot water into a predetermined shape.
Incense containing 5% by weight of cocoon is based on 40% by mass of coconut shell charcoal powder, 25% by mass of binder containing tab bark powder, 30% by mass of alumina oxide powder, and 5% by mass of cocoon powder. An incense stick formed into a predetermined shape after adding an appropriate amount of water or hot water and kneading well.
Further, the incense containing 10% by mass of cocoon is based on 40% by mass of coconut shell charcoal powder, 25% by mass of binder containing tab bark powder, 25% by mass of alumina oxide powder, and 10% by mass of cocoon powder. An incense stick formed into a predetermined shape after adding an appropriate amount of water or hot water and kneading well.

  As described above, cocoon is not an excellent fragrance unlike clove or hiba oil, and is generally a herbal medicine that is unlikely to be blended with incense as a fragrance. It can be confirmed that remarkable antibacterial activity is exhibited against the bacteria of (2) to (3) and (6) to (9).

  In particular, the antibacterial scent according to the present invention containing cocoon is a gram-positive bacterium as shown in FIG. 1 (b), while antibiotics and herbal medicines showing antibacterial activity effective against gram-positive bacteria and fungi are rare. E. E. coli ATCC 25922, a fungus shown in FIG. albicans ATCC 10231, which is also a fungus, as shown in FIG. It can be confirmed that it exhibits remarkable antibacterial activity against albicans ATCC10261.

  In addition, although shown in Table 1 about the antibacterial effect with respect to the said (1), (4), (5) microbe, the antibacterial fragrance | flavor which concerns on this invention which mix | blended the soot from the experimental data shown in Table 1 is It can be confirmed that the antibacterial activity of the above-mentioned (1), (4) and (5) is also exhibited, and generally exhibits a broad antibacterial spectrum of (1) to (9).

  In other words, the antibacterial scent according to the present invention containing sputum exhibits a broad antibacterial action against bacteria and fungi that are persistent in general life and cause food poisoning, cutaneous suppuration disease, opportunistic infections, etc. By using the antibacterial scent according to the present invention in which cocoon is added, various infectious diseases caused by these bacteria can be widely prevented.

  FIG. 2 is a graph showing the antibacterial effect of an incense containing 5% by weight of clove as an antibacterial component, and a remarkable antibacterial activity was recognized compared to an incense that does not contain any crude drugs or essential oils (comparative example). It is the graph which showed the suppression rate with respect to the microbe of said (2)-(3) and (6)-(9). In addition, the suppression rate of the incense which mix | blended 5% of clove is shown with the white square ((square)). Incense containing 5% of clove is an appropriate amount of water or 40% by mass of coconut shell charcoal powder, 25% by mass of binder containing tab bark powder, 30% by mass of alumina oxide powder, and 5% by mass of clove powder. An incense stick formed into a predetermined shape after adding hot water and kneading well.

  As described above, clove is a herbal medicine that is excellent in aromaticity and has been conventionally used as a fragrance in a scent, and is a herbal medicine that has been used as an antiseptic sterilizing effect in addition to a scent. Although the antibacterial effect on the bacterium has not been scientifically proven, when the antibacterial effect is tested according to the above test method, it is prominent against the bacteria of the above (2) to (3) and (6) to (9) It can be confirmed that it exhibits antibacterial activity.

  In particular, incense that contains antibacterial activity effective against Gram-positive bacteria is rare, and the scent blended with clove is an E. coli that is a Gram-positive bacteria shown in FIG. It can be confirmed that it exhibits remarkable antibacterial activity against E. coli ATCC 25922.

  In addition, although shown in Table 1 about the antibacterial effect with respect to the said (1), (4), (5) microbe, the incense which mix | blended the clove from the experimental data shown in Table 1 is said (1) , (4) and (5) can also be confirmed to exhibit a predetermined antibacterial activity and generally exhibit a broad antibacterial spectrum of (1) to (9).

  FIG. 3 is a graph showing the antibacterial effect of an incense containing 5% by weight of hiba oil as an antibacterial component, and remarkable antibacterial activity was recognized as compared with an incense that does not contain any crude drugs or essential oils (comparative example). It is the graph which showed the suppression rate with respect to the microbe of said (2)-(3) and (6)-(9). The incense suppression rate containing 5% hiba oil is shown by white rhombus (◇). Incense containing 5% of Hiba oil is composed of 40% by mass of coconut shell charcoal powder, 25% by mass of binder containing tab bark powder and 35% by mass of alumina oxide powder. An incense stick formed into a predetermined shape after adding hot water and kneading well.

As mentioned above, Hiba oil is an essential oil that has been used as a fragrance for soaps, toothpastes, and toiletries because its hinokitiol component has antibacterial properties, but its antibacterial effects against specific fungi are scientifically proven. However, when the antibacterial effect is tested according to the above test method, it is confirmed that the antibacterial activity is remarkable against the bacteria of the above (2) to (3) and (6) to (9). Can do.
In particular, S. cerevisiae, which is a gram-negative bacterium shown in FIG. It can be confirmed that it exhibits remarkable antibacterial activity against Aureus ATCC 25923.

  In addition, although it shows in Table 1 about the antibacterial effect with respect to the microbe of said (1), (4), (5) and is not illustrated, the fragrance | flavor which mix | blended hiba oil from the experimental data shown in Table 1 is said (1). , (4) and (5) can also be confirmed to exhibit a predetermined antibacterial activity and generally exhibit a broad antibacterial spectrum of (1) to (9).

  FIG. 4 is a graph showing the antibacterial effect of the antibacterial scent according to the present invention in which koji and hiba oil are blended as an antibacterial component (mixture of two types), and the above (5), (7 It is the graph which showed the suppression rate with respect to the microbe of () and (8). The black circle (●) indicates the inhibition rate of the antibacterial incense according to the present invention in which 10% cocoon and 5% hiba oil are mixed by mass%, and the white circle (◯) indicates the inhibition rate of the incense containing 10% alone. The rhombus (◇) shows the inhibition rate of incense containing 5% of Hiba oil alone for comparison. The incense blended with straw and hiba oil is hiba oil based on 40% by weight of coconut shell charcoal powder, 25% by weight of binder containing tab bark powder, 25% by weight of alumina oxide powder, and 10% by weight of straw powder. An incense stick formed into a predetermined shape after adding 5% by mass and an appropriate amount of water or hot water and kneading well.

  FIG. 4A shows the bacterium of the above (5), that is, E. coli. It is the graph which showed the suppression rate with respect to faecalis ATCC19433, and incense length 9cm, the fragrance | flavor which mix | blended strawberry alone showed the suppression rate of 5.0%, and the incense which mix | blended hiba oil showed 4.4% suppression rate. However, it can be confirmed that the antibacterial fragrance obtained by mixing two kinds of the present invention according to the present invention exhibits a suppression rate of 15.1% and exhibits synergistic activity by mixing the two kinds.

  FIG. 4 (b) shows the bacteria of (7) above, that is, S. cerevisiae. It is the graph which showed the inhibition rate with respect to aureus ATCC25923, and incense length 3cm, the incense which mix | blended strawberry alone shows 0.6% inhibition rate, and the incense which mix | blended hiba oil shows 0.4% inhibition rate However, it can be confirmed that the antibacterial fragrance obtained by mixing two types of the present invention according to the present invention exhibits a suppression rate of 40.9% and exhibits synergistic activity by mixing the two types.

  Furthermore, FIG. 4 (c) shows the bacterium of the above (8), that is, C.I. It is the graph which showed the suppression rate with respect to albicans ATCC10231, and incense stick length 3cm, the incense which mix | blended strawberry alone shows a suppression rate of 47.1%, and the incense which mix | blended hiba oil shows the suppression rate of 0%. It can be confirmed that the antibacterial fragrance in which the two types of the present invention are mixed has an inhibition rate of 75.7% and shows synergistic activity by mixing the two types.

  In addition, although it shows in Table 1 about the antibacterial effect with respect to microbes other than the above, although not shown in figure, from the experimental data shown in Table 1, the antibacterial fragrance | flavor which concerns on this invention which mix | blended (mixed 2 types) with a camellia and a hiba oil is ( It can be confirmed that a broad antibacterial spectrum of 1) to (9) is exhibited.

  That is, the antibacterial incense according to the present invention containing cocoon and hiba oil (mixed in two kinds) has a broad antibacterial action against bacteria and fungi that are permanently present in general life and cause food poisoning, cutaneous suppuration diseases, opportunistic infections, etc. As well as exhibiting synergistic activity by mixing two kinds, it is possible to widely prevent various infectious diseases caused by these bacteria by daily use of the incense.

  FIG. 5 is a graph showing the antibacterial effect of the antibacterial incense according to the present invention in which candy, clove and hiba oil are blended as antibacterial components (mixed in three types), and the synergistic activity by mixing the three types is recognized (5), It is the graph which showed the suppression rate with respect to the microbe of (7), (8) and (9). The black triangle (▲) indicates the inhibition rate of the antibacterial incense according to the present invention in which 5% each of cocoon, clove and hiba oil are mixed in mass%, and the white triangle (△) indicates the inhibition rate of the incense containing 5% alone. The white square (□) shows the inhibition rate of incense containing 5% of clove alone, and the white rhombus (◇) shows the inhibition rate of incense containing 5% of hiba oil for comparison. The incense containing straw, clove and hiba oil is 40% by weight of coconut shell charcoal powder, 25% by weight of binder containing tab bark powder, 25% by weight of alumina oxide powder, 5% by weight of straw powder, 5% by weight of clove powder. An incense stick formed into a predetermined shape after adding 5% by mass of hiba oil and an appropriate amount of water or hot water to the powder raw material 100 and kneading well.

  FIG. 5A shows the bacterium of the above (5), that is, E. coli. It is the graph which showed the suppression rate with respect to faecalis ATCC19433, and incense stick length 9cm, the incense which mix | blended candy alone has the suppression rate of 4.7%, the incense which mix | blended clove only has the suppression rate of 4.6%, And the incense containing Hiba oil alone shows a suppression rate of 4.4%, and the antibacterial incense mixed with 3 types according to the present invention shows a suppression rate of 18.8% and shows synergistic activity by mixing 3 types. Can be confirmed.

  FIG. 5 (b) shows the bacteria of (7) above, that is, S. cerevisiae. It is the graph which showed the suppression rate with respect to aureus ATCC25923, and incense stick length 3cm, the incense which mix | blended candy alone has the suppression rate of 0.2%, the incense which mix | blended clove only has the suppression rate of 0.6%, And the incense in which Hiba oil is blended alone shows a suppression rate of 0.4%, the antibacterial incense mixed with 3 types according to the present invention shows a suppression rate of 12.0% and shows synergistic activity by mixing 3 types. Can be confirmed.

  Furthermore, FIG. 5 (c) shows the bacterium of the above (8), that is, C.I. It is the graph which showed the suppression rate with respect to albicans ATCC10231, and incense stick length 3cm, the incense which mix | blended strawberry alone has the suppression rate of 41.4%, the incense which mix | blended clove only has the suppression rate of 2.8%, And the incense with Hiba oil alone shows 0% inhibition rate, the antibacterial incense mixed with 3 kinds according to the present invention shows 84.3% inhibition rate and shows synergistic activity by mixing 3 kinds can do.

  Furthermore, FIG. 5 (d) shows the bacteria of (9) above, that is, C.I. It is the graph which showed the inhibition rate with respect to albicans ATCC10261, and incense stick length 3cm, the incense which mix | blended strawberry alone has the inhibition rate of 17.6%, the incense which mix | blended clove alone has the inhibition rate of 0%, and hiba oil It is confirmed that the incense in which the scent is mixed alone shows 0% inhibition rate, the antibacterial incense mixed with 3 kinds according to the present invention shows 44.5% inhibition rate and shows synergistic activity by mixing 3 kinds. Can do.

  In addition, although it shows in Table 1 about the antibacterial effect with respect to microbes other than the above, it does not show in figure, from the experimental data shown in Table 1, the antibacterial fragrance | flavor which concerns on this invention which mix | blended (mixed 3 types) of strawberry, clove, and hiba oil , (1) to (9) can be confirmed to show a broad antibacterial spectrum.

  That is, the antibacterial incense according to the present invention containing strawberry, clove and hiba oil (mixed in three kinds) is wide against bacteria and fungi that are resident in general life and cause food poisoning, cutaneous suppuration disease, opportunistic infection, etc. Since it exhibits an antibacterial action and exhibits synergistic activity by mixing three kinds, it is possible to widely prevent various infectious diseases caused by these bacteria by using the perfumes on a daily basis.

Next, cocoon blended as an antibacterial component is not excellent in fragrance unlike clove and hiba oil as described above, and is generally a crude drug that is unlikely to be blended in fragrance as a fragrance.
It is desirable to use the dried rhizomes of Asterylodes lancea DC. Or Atractylodes chinensis Koidz. Also, dried rhizomes of A. lancea DC. Var. Chinensis Kitamura can be used.
As a compounding quantity of soot, it is desirable to mix | blend 1-35% by mass%, More preferably, it is more desirable to mix | blend 5-20% by mass%. If it is less than 1%, a sufficient antibacterial effect cannot be obtained, and if it exceeds 35%, the unique scent of candy becomes so strong that it is not suitable for indoor use. If the mass% is 5 to 20%, as shown in FIG. 1, it is possible to stably exert a broad antibacterial action against bacteria and fungi that cause opportunistic infections, etc. . In addition, the said compounding quantity is common to all the antibacterial fragrance | flavor which concerns on this invention, and also when mix | blending strawberry alone and when mixing with clove and hiba oil.

    As a blending amount of clove (CARYOPHYLLI FLOS) blended as an antibacterial component, it is desirable to blend 1 to 35% by mass%, and it is more desirable to blend 5 to 20% by mass%. If it is less than 1%, sufficient antibacterial effect cannot be obtained, and if it exceeds 35%, the scent becomes too strong to be suitable for indoor use. If the mass% is 5 to 20%, as shown in FIG. 2, a wide antibacterial action against bacteria and fungi causing opportunistic infections can be stably exhibited, and the rich aroma of clove can be enjoyed.

  Hiba oil is an essential oil obtained by steam distillation of roots or branches and leaves of the plant of the cypress Asunaro genus Asubaro (Thujopsis dolabrata Sieb. Et Zucc.). It is desirable to blend 0.5 to 20% by mass%, and it is more desirable to blend 3 to 10% by mass%. If it is less than 0.5%, a sufficient antibacterial effect cannot be obtained and the refreshing scent of hiba cannot be enjoyed. If it exceeds 20%, the scent becomes too strong and it becomes difficult to mold. If the mass% is 3 to 10%, as shown in FIG. 3, a wide antibacterial action against bacteria and fungi causing opportunistic infections can be stably exhibited, and the fresh aroma of hiba can be enjoyed.

  The antibacterial action of the antibacterial scent according to the present invention is exhibited when the antibacterial component blended in the scent is vaporized by burning and diffuses in the indoor space. Therefore, as long as it is an antibacterial incense burned by direct fire, it is not limited to the form of an incense stick having a length of 3 to 15 cm used in the antibacterial test. For example, when used in a wide indoor space or a space where there is a large amount of air flow, it is effective to use a coil-type antibacterial scent since the combustion time is long. In addition, when it is desired to exhibit an antibacterial effect in a short time, it is effective to use a corn type antibacterial incense that vaporizes the antibacterial component in a short time. In addition, stick-type or chip-type antibacterial incense is easy to handle and can be used on a daily basis, so it is effective when used at home. Furthermore, when it is made in the form of incense and burnt incense, it can create a strict atmosphere at religious events that are the traditional way of using incense, and can direct the scent to the Buddha, and at the same time has antibacterial action It can be demonstrated.

  The antibacterial incense that is one form of the antibacterial incense according to the present invention can be manufactured by the following method. First, powdery raw materials are mixed in a predetermined composition, and water or hot water in an amount necessary for molding is mixed therein and kneaded. When adding an oily raw material, that is, hiba oil, it is added here. Next, a linear shaped product can be obtained by forcibly passing the kneaded material kneaded into a clay shape through a nozzle hole having a predetermined thickness. And an antibacterial incense can be obtained by drying the said molding. Further, by making the shape of the nozzle hole round or square, a stick-shaped scent of that shape can be obtained.

  In addition, for antibacterial incense that is one form of the antibacterial incense according to the present invention, powdery raw materials are mixed in a predetermined composition, and when adding oily raw materials, the antibacterial incense is obtained by coating the powdered raw materials. Can do.

  Furthermore, the antibacterial incense which is one form of the antibacterial incense according to the present invention can obtain the antibacterial incense by cutting a predetermined raw material into chips of about 0.5 to 2 mm and mixing them. Moreover, when adding an oil-form raw material, it can add by sprinkling well at the time of mix | blending. Alternatively, powdery raw materials are mixed in a predetermined composition, and an amount of water or hot water necessary for molding is added thereto and kneaded. If you want to put oily raw material, put it here. And the antibacterial incense can be obtained also by shape | molding the raw material kneaded in clay shape in a sheet form, and crushing the sheet | seat in the chip shape of about 0.5-2 mm.

It is a graph which shows the antibacterial effect of the antibacterial incense which concerns on this invention which mix | blended the candy as an antibacterial component. It is a graph which shows the antibacterial effect of the antibacterial incense which mix | blended clove as an antibacterial component. It is a graph which shows the antibacterial effect of the antibacterial incense which mix | blended hiba oil as an antibacterial component. It is a graph which shows the antibacterial effect of the antibacterial incense which concerns on this invention which mix | blended camellia and hiba oil as an antibacterial component. It is a graph which shows the antibacterial effect of the antibacterial incense based on this invention which mix | blended camellia, clove, and hiba oil as an antibacterial component.

Claims (3)

An antibacterial ingredient with a broad antibacterial spectrum characterized by the addition of sputum as an antibacterial ingredient in an antibacterial incense that vaporizes the antibacterial ingredient blended by combustion and exerts an antibacterial effect by the vaporized antibacterial ingredient diffusing through the indoor space Incense.
The antibacterial fragrance having a broad antibacterial spectrum according to claim 1, further comprising Hiba oil as an antibacterial component.
Furthermore, clove is mix | blended as an antibacterial component, Antibacterial incense with a wide antibacterial spectrum of Claim 2 characterized by the above-mentioned.

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