JP2007295833A - Life style disease-preventive food containing frozen dry powder of dioscorea batatas - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide frozen dry powder of Dioscorea batatas comprising food raw material having a function preventing or ameliorating life style disease caused by inadequate dietary habit or lack of exercise. <P>SOLUTION: The life style disease-preventive or -ameliorating food contains uncooked Dioscorea batatas powder. Earnest effort to solve the life style disease results in finding that the uncooked Dioscorea batatas powder has the function preventing or ameliorating the life style disease so as to accomplish the invention. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to the use of non-heated long powder as a functional food.

  There are various illnesses and symptoms in lifestyle-related diseases, and what kind of illness develops and how fast it progresses after onset varies depending on individual constitution and lifestyle. The terrible point of lifestyle-related diseases is that many of them have almost no subjective symptoms over a long period of time, and the symptoms progress unknowingly. For example, “hyperlipidemia” “hypertension” “diabetes” Can be mentioned. If these diseases are left as they are, for example, a blood vessel malfunction such as “arteriosclerosis” is caused, and the risk of eventually leading to serious life-related diseases such as heart disease and stroke increases. In addition, these three diseases may occur alone, but each may cause and result in complications, and the treatment increases the degree of difficulty, so-called “quality of life (QOL)”. ) ”Will be drastically reduced.

  Accordingly, the development of food components having physiological functions that improve risk factors for the development of lifestyle-related diseases such as hyperglycemia, hyperinsulinemia, and hyperlipidemia is awaited widely.

  The physiological function of the powder was not known even though it was not heated.

  Provided is a food material having an action to prevent or ameliorate lifestyle-related diseases caused by inappropriate eating habits or lack of exercise.

As a result of diligent efforts to solve the above-mentioned problems, the present inventors have found that non-heated and long powder has an action of preventing or improving lifestyle-related diseases, and completed the present invention.
That is, the present invention relates to a food for preventing lifestyle-related diseases or a food for improving lifestyle-related diseases, which includes a non-heated long powder.

  That is, the non-heated long powder of the present invention can be obtained by lyophilizing a long unheated powder. The composition of the non-heated long powder of the present invention is, for example, 1.2 g water, 10.1 g protein, 0.6 g fat, 83.4 g carbohydrate (5.1 g total dietary fiber, 1.9 g water-soluble dietary fiber per 100 g). Insoluble dietary fiber 3.2 g), ash content 4.7 g, energy 379 kcal.

  Any non-heated long powder as a raw material for producing powder of the present invention can be used as long as it is conventionally known.

  The functional food of the present invention only needs to contain the non-heated long powder according to the present invention. Therefore, the food which consists only of the said non-heated long powder may be sufficient, and the food which contains components other than the said non-heated long powder may be sufficient. The components other than the non-heated long powder of the present invention are not particularly limited as long as they do not inhibit the lipid metabolism regulating action. Specific examples include plant and animal proteins, carbohydrates, dietary fiber, lipids, various vitamins, minerals, and the like. The shape of the food is not particularly limited, and may be various shapes such as a solid, liquid, powder, and paste.

The functional food material of the present invention is a food having an effect of improving lipid metabolism because it contains the above non-heated long powder. Therefore, the food has an action of preventing and ameliorating lifestyle-related diseases caused by inappropriate eating habits or lack of exercise. Examples of lifestyle-related diseases resulting from the inappropriate diet or lack of exercise include obesity, hyperlipidemia, hypertension, and diabetes, and the food of the present invention is obesity, hyperlipidemia, hypertension or It has an action to prevent and ameliorate at least one of diabetes. Further, this lifestyle-related disease prevention / improvement effect is specifically the action of at least one of an increase in blood HDL cholesterol concentration, a decrease in blood LDL cholesterol concentration, and a decrease in blood triglyceride concentration, ie, lipid metabolism regulation. This is an effect obtained by the action.

  Specific examples of the food according to the present invention include tablets, granules, powders, drinks and the like containing the food material according to the present invention as so-called nutritional supplements (supplements). In addition, the seasoning containing the said foodstuff material, confectionery, bread | pan, side dish, drinking water etc. can be mentioned.

  Needless to say, the food materials, foods, and pharmaceuticals according to the present invention are intended for humans, but are not limited to humans and can be used for a wide range of animals. In particular, pets such as dogs and cats who are suffering from inappropriate eating habits and lack of exercise are suitable as targets.

  The present invention also relates to a pharmaceutical comprising the peptide mixture according to the present invention and having an action of preventing and ameliorating at least one of obesity, hyperlipidemia, hypertension and diabetes. The component contained other than the peptide mixture is not particularly limited. The pharmaceutical of the present invention is preferably in a dosage form that can be administered orally. Specific examples include tablets, granules, powders, drinks, and syrups.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, this invention is not limited by these Examples.

Example 1 (Production of non-heated long powder)
The long piece was washed, peeled, further cut to a width of 3 cm, ground using a grinder, frozen at −40 ° C., and lyophilized. After lyophilization, the sample was prepared by pulverizing with a pulverizer.

Example 2 (Influence on rats)
Animals used: Six Sprague-Dawley (SD) male rats aged 4 weeks were used per group.
Acclimatization and administration: After 10 days of preliminary breeding, daily long potato powder was mixed with food and administered orally for 21 days.
Feed composition: Rat feed composition table Control feed 93 was used as a feed for the control group.

  In the administration group, non-heated long potato powder was replaced with 15% and 30%, respectively, instead of pregelatinized corn starch. Further, a group was also provided in which heated long potato powder (boiled with hot water for 1 minute) was replaced with 15% and 30%, respectively. The grouping is so that there is no significant difference in body weight and plasma cholesterol concentration, control food group, 15% non-heated long potato food group, 30% non-heated long potato food group, 15% heated long potato food group, 30% heated length Six groups of potato food groups were provided. Rats were individually housed under the following conditions: room temperature 23 ± 1 ° C., humidity 60 ± 5%, and light / dark cycle 12 hours.

  Measurement items: Blood was collected from the tail vein every week, and plasma total cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, neutral fat, free fatty acid, and phospholipid concentrations were measured. Plasma total cholesterol, HDL-cholesterol, LDL-cholesterol and VLDL-cholesterol are enzymatic methods (cholesterol oxidase / DAOD method), and neutral fats are enzymatic methods (clinimate TG-2 reagent kit, Daiichi Kagaku). Was measured by the ACS (acyl-CoA synthetase) / ACOD (acyl CoA oxidase) method, and the phospholipid was measured by the Fiske-Sarovar method using a Climate IP reagent kit (Daiichi Kagaku) after lipid extraction. Further, feces were collected for 3 days on the last day of administration and their weights were measured. After dissection, the liver, accessory testicular fat, testicles, and cecum were removed and their weights were measured.

Short chain fatty acid analysis: The cecal contents were extracted from the excised cecum into a conical tube using tweezers, and the weight of the cecal contents was measured. Thereafter, the pH was measured while stirring the contents of the cecum. Next, the contents of the cecum were made up to 5 ml with sterile ultrapure water and mixed, and then 1.5 ml of the mixed solution was transferred to a 1.5 ml centrifuge tube and centrifuged at 10,000 rpm for 10 minutes. 1 ml of this supernatant was transferred to another centrifuge tube, mixed with 50 μl of cold 70% HClO ₄ and then centrifuged at 4 ° C., 10,000 rpm for 10 minutes to precipitate the protein. Furthermore, 500 μl of this supernatant was transferred to another tube, mixed with 100 μl of 4N NaOH, and centrifuged at 4 ° C. and 10,000 rpm for 10 minutes. 100 μl of the supernatant was transferred to another tube, and 100 μl of 3N H ₃ PO ₄ was added and mixed. 2 μl of this was subjected to gas chromatography to quantify short chain fatty acids.

Results Table 1 shows plasma lipid concentrations of abdominal aortic blood at the time of dissection. Unlike cholesterol variation in tail vein plasma, no significant difference was observed in total cholesterol concentration. However, the plasma cholesterol concentration in the 30RY group tended to decrease. The VLDL-cholesterol concentration was significantly reduced in the 30RY group compared to the control food group. The arteriosclerosis index (AI) was significantly lower in the 30RY group than in the control diet group. The triglyceride concentration was significantly decreased in the 15RY group, the 30RY group, and the 30BY group as compared to the control food group, and a decrease was observed between the RY groups in a dose-dependent manner. The phospholipid concentration was significantly reduced only in the 30RY group compared to the control diet group.

  A decrease in plasma lipid concentration in the 30RY group was observed at any lipid concentration, suggesting the possibility that long-lived may regulate lipid metabolism by several mechanisms.

  Table 2 shows the amount and concentration of short chain fatty acids and succinic acid in the cecal contents. Since the cecum of rats fed with raw long potato (RY) was enlarged, the amount of short-chain fatty acids in the cecal contents was significantly increased by RY intake. The increase was volume dependent. Although the amount of succinic acid varied, it significantly increased in the 30RY group compared to the control food group. Although some of the short-chain fatty acids in the boiled buttery (BY) -treated rats were also partially increased, they were significantly lower than those in the RY-treated rats.

  On the other hand, the concentrations of acetic acid and propionic acid, which are short-chain fatty acids, were hardly different among the treatment groups except for 30RY group propionic acid. The butyric acid concentration was significantly increased 2- to 2.5-fold in rats given a long time regardless of the presence or absence of heat treatment. For this reason, the ratio of short-chain fatty acids in the cecum contents was mainly acetic acid in the control diet group, but the ratio of acetic acid to (propionic acid + butyric acid) became 5: 5 by ingestion for a long time. confirmed. This change is presumed to be due to the increase in butyric acid fermentation. It is known that butyric acid is indispensable for the growth of colonic epithelial cells and has a colon cancer inhibitory effect.

That is, the following effects were recognized by these experiments.
Plasma cholesterol levels were significantly reduced in the 30% non-heated long diet group.
The cecal contents, cecal wall weight, and short chain fatty acid concentration increased significantly with ingestion of non-heated long substances (FIG. 2). On the other hand, the pH of the cecum content decreased depending on the intake amount of the non-heated long substance (FIG. 3), and fermentation was promoted by the long intake.
Fecal bile acid excretion was significantly increased in both groups ingested heated long potato compared to the control diet group, and no change was observed in both groups ingested unheated long potato. For this reason, the decrease in plasma cholesterol concentration observed in the 30% unheated long potato group is not due to increased bile acid excretion, but is due to fluctuations in cholesterol uptake into the liver or liver cholesterol synthesis. It is inferred that

  The food of the present invention is useful for preventing or ameliorating lifestyle-related diseases caused by inappropriate eating habits or lack of exercise.

Plasma cholesterol concentration fluctuation Cecal contents and cecal wall weight Cecal content pH, fecal total bile acid content

Claims (1)

  A food for preventing lifestyle-related diseases or a food for improving lifestyle-related diseases, comprising non-heated long powder.

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