JP2007292527A - Microchip and system for detecting chemical reaction - Google Patents

Microchip and system for detecting chemical reaction Download PDF

Info

Publication number
JP2007292527A
JP2007292527A JP2006118691A JP2006118691A JP2007292527A JP 2007292527 A JP2007292527 A JP 2007292527A JP 2006118691 A JP2006118691 A JP 2006118691A JP 2006118691 A JP2006118691 A JP 2006118691A JP 2007292527 A JP2007292527 A JP 2007292527A
Authority
JP
Japan
Prior art keywords
reaction
microchip
flow path
fluid
reservoir
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2006118691A
Other languages
Japanese (ja)
Inventor
Hisao Nakanishi
久雄 中西
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Bakelite Co Ltd
Original Assignee
Sumitomo Bakelite Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Bakelite Co Ltd filed Critical Sumitomo Bakelite Co Ltd
Priority to JP2006118691A priority Critical patent/JP2007292527A/en
Publication of JP2007292527A publication Critical patent/JP2007292527A/en
Pending legal-status Critical Current

Links

Landscapes

  • Micromachines (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microchip capable of reducing variation of reaction results, and to provide a chemical reaction detecting system. <P>SOLUTION: The microchip has a reaction part for the purpose of chemical reaction or biochemical reaction, a flow channel with a width of 1 μm to 1 cm, an inlet hole capable of introducing a fluid into the flow channel and a discharge hole, capable of discharging the fluid from the flow channel. Furthermore, a reservoir part for storing a reagent used in the reaction is provided in the same microchip and the inlet hole, the reservoir part and the reaction part are arranged in this order and are made electrically continuous to each other by the flow channel. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

本発明は、有機化合物、生体試料などの微量試料を混合、反応、合成、抽出、分離する事を目的としたマイクロチップであってそれら操作結果を検出する事が可能な化学反応検出システムに関する。   The present invention relates to a chemical reaction detection system that is a microchip intended to mix, react, synthesize, extract, and separate a trace amount sample such as an organic compound and a biological sample and detect the operation results.

有機化学、生化学分野において混合、反応、合成、抽出、分離、分析について高速化、微少試料、微小空間での操作が注目されており、その技術を確立するためにマイクロチップの研究が精力的に進められている。
一般的にマイクロチップ は、微細な流路をもったガラス基板と試料を導入及び排出する孔をもったガラス基板の２枚が接合されたものである。 In the fields of organic chemistry and biochemistry, mixing, reaction, synthesis, extraction, separation, and analysis have been attracting attention for high-speed operation, small samples, and operation in microspaces, and research on microchips has been vigorous to establish that technology. It is advanced to.
In general, a microchip is formed by bonding a glass substrate having a fine channel and a glass substrate having holes for introducing and discharging a sample.

マイクロチップ内で前述した混合、反応、合成、抽出、分離（以後、これら５工程を反応と略す）が行われた後、それらによって得られた生成物について該マイクロチップ内で分析・検出（以後、これら２工程を検出と略す）を行う。 前述の反応・検出を行う際、一般的に反応に必要な試薬を順に送液する。マイクロチップ外に試薬を貯蔵するリザーバーを設け試薬をポンプを用いて送液する。試薬を別の試薬へ変更する度にポンプの入れ替えや流路の洗浄工程が必要になる。また、操作の際に気泡や不純物が混入しやすく反応結果のばらつきの原因となる。
この課題の対策としてリザーバーを１枚のマイクロチップ内に封入する方法があるが、試薬を入れ替える際に流路の分岐の部分に先に送液した試薬が残留し反応結果のばらつきの原因となる。 After the above-described mixing, reaction, synthesis, extraction, and separation (hereinafter, these five steps are abbreviated as reactions) are performed in the microchip, the products obtained by these are analyzed and detected in the microchip (hereinafter referred to as “reactions”). These two steps are abbreviated as detection). When performing the above-described reaction / detection, reagents necessary for the reaction are generally sent in order. A reservoir for storing the reagent is provided outside the microchip, and the reagent is fed using a pump. Each time the reagent is changed to another reagent, a pump replacement and a flow path cleaning step are required. In addition, bubbles and impurities are likely to be mixed during the operation, causing variations in reaction results.
As a countermeasure to this problem, there is a method of enclosing a reservoir in one microchip. However, when the reagent is replaced, the reagent that has been sent first remains in the branch of the flow path, causing variations in reaction results. .

特開２００１−４６２８号公報JP 2001-4628 A

本発明の目的は、操作の際に気泡や不純物の混入を防ぎ、反応結果のばらつきを軽減する事ができるマイクロチップ及び化学反応検出システムを提供することである。   An object of the present invention is to provide a microchip and a chemical reaction detection system capable of preventing bubbles and impurities from being mixed during operation and reducing variations in reaction results.

本発明は、以下の通りである。
（１）幅が１μｍ〜１ｃｍの流路、前記流路に流体を導入可能な導入孔、及び前記流路から流体を排出可能な排出孔とを有するマイクロチップであって、更に化学反応又は生化学反応に使用する試薬を蓄えるためのリザーバー部を同一チップ内に有し、かつ前記流路によって導通していることを特徴とするマイクロチップ。
（２）化学反応又は生化学反応を目的とした反応部、幅が１μｍ〜１ｃｍの流路、前記流路に流体を導入可能な導入孔、及び前記流路から流体を排出可能な排出孔を有するマイクロチップであって、更に前記反応に使用する試薬を蓄えるためのリザーバー部を同一チップ内に有し、導入孔、リザーバー部、反応部がこの順に配置され、かつ前記流路によって導通していることを特徴とするマイクロチップ。
（３）前記リザーバー部と前記反応部との間に更に流体の流量の調節又は流体の送液／停止を切り替えることができるバルブを有する（２）記載のマイクロチップ。
（４）前記流路が分岐部がないものであり、前記流路内にリザーバー部及び反応部が形成されている（２）又は（３）記載のマイクロチップ。
（５）（１）記載のマイクロチップと、化学反応又は生化学反応を目的とした反応部、幅が１μｍ〜１ｃｍの流路、前記流路に流体を導入可能な導入孔、及び前記流路から流体を排出可能な排出孔を有する第２のマイクロチップとを導通手段により連結して構成される化学反応検出システム。
（６）（１）〜（４）いずれか記載のマイクロチップに化学反応又は生化学反応に使用する試薬を導入し、使用するまでは凍結保存し、使用直前に解凍する事によって化学反応又は生化学反応を行う事を特徴とする化学反応検出方法。 The present invention is as follows.
(1) A microchip having a flow path having a width of 1 μm to 1 cm, an introduction hole through which fluid can be introduced into the flow path, and a discharge hole through which fluid can be discharged from the flow path. A microchip having a reservoir part for storing a reagent used for a chemical reaction in the same chip and being connected by the flow path.
(2) a reaction section for the purpose of chemical reaction or biochemical reaction, a flow path having a width of 1 μm to 1 cm, an introduction hole capable of introducing a fluid into the flow path, and a discharge hole capable of discharging the fluid from the flow path The microchip further includes a reservoir part for storing the reagent used for the reaction in the same chip, and the introduction hole, the reservoir part, and the reaction part are arranged in this order, and are conducted by the flow path. A microchip characterized by
(3) The microchip as set forth in (2), further comprising a valve capable of switching the flow rate of the fluid or switching the liquid feeding / stopping between the reservoir unit and the reaction unit.
(4) The microchip according to (2) or (3), wherein the flow path has no branch part, and a reservoir part and a reaction part are formed in the flow path.
(5) The microchip according to (1), a reaction part intended for chemical reaction or biochemical reaction, a channel having a width of 1 μm to 1 cm, an introduction hole through which fluid can be introduced into the channel, and the channel A chemical reaction detection system configured by connecting a second microchip having a discharge hole capable of discharging a fluid from a liquid by a conduction means.
(6) The reagent used for the chemical reaction or biochemical reaction is introduced into the microchip according to any one of (1) to (4), stored frozen until use, and thawed immediately before use. A chemical reaction detection method characterized by performing a chemical reaction.

本発明に従うと、前述の反応・検出を行う際、試薬を別の試薬へ変更する度にポンプの入れ替えや流路の洗浄工程が不要になる為、操作の際に気泡や不純物の混入を防ぐことができ反応結果のばらつきを軽減する事ができる。
また、導入孔、流路、リザーバー部、流路、反応部が順に並んでおり流路の分岐がない為、分岐点における試薬の残留を防ぐ事ができ、反応結果のばらつきを軽減する事ができる。 According to the present invention, when performing the above-described reaction / detection, it is not necessary to replace the pump or clean the flow path every time the reagent is changed to another reagent, thereby preventing air bubbles and impurities from being mixed during operation. Variation of reaction results can be reduced.
In addition, since the introduction hole, flow path, reservoir section, flow path, and reaction section are arranged in order and there is no branch of the flow path, it is possible to prevent the reagent from remaining at the branch point and reduce the variation in the reaction results. it can.

本発明のマイクロチップの一実施態様を図１に示す。図１は、導入孔２、リザーバー部３、５、７、バルブ４１、４２、４３、反応部８が順に形成され、微細な流路により分岐部がなく導通されているマイクロチップである。   One embodiment of the microchip of the present invention is shown in FIG. FIG. 1 shows a microchip in which an introduction hole 2, reservoir portions 3, 5, 7, valves 41, 42, 43, and a reaction portion 8 are formed in this order and are connected by a fine flow path without a branching portion.

本発明のマイクロチップに使用する素材はガラス又はプラスチックが好ましい。   The material used for the microchip of the present invention is preferably glass or plastic.

本発明のリザーバー部は、化学反応又は生化学反応に使用する試薬を蓄えるためのチップ内に形成される部位である。リザーバー部の形状は流路同様に溝形状でも構わないし円柱状、半球上でも差し支えない。抗原抗体反応に使用する場合の試薬は、標識抗体溶液、抗原溶液、及び洗浄剤等である。   The reservoir portion of the present invention is a site formed in a chip for storing a reagent used for a chemical reaction or a biochemical reaction. The shape of the reservoir portion may be a groove shape as in the flow path, or may be a cylindrical shape or a hemisphere. Reagents for use in the antigen-antibody reaction include a labeled antibody solution, an antigen solution, and a cleaning agent.

リザーバー部と反応部が同一チップ内に形成されている場合、リザーバー部と反応部との間に更に流体の流量の調節又は流体の送液／停止を切り替えることができるバルブを有することが好ましい。   When the reservoir portion and the reaction portion are formed in the same chip, it is preferable to further include a valve capable of switching the flow rate of the fluid or switching the fluid supply / stop between the reservoir portion and the reaction portion.

バルブは小型化を考慮するとダイヤフラム式バルブ、電磁バルブ、又は手動式回転バルブが好ましい。特に簡便なものとして、シリコンゴム等の素材で形成された３方弁を有する３方弁バルブが挙げられる。   In view of miniaturization, the valve is preferably a diaphragm valve, an electromagnetic valve, or a manual rotary valve. A particularly simple one is a three-way valve valve having a three-way valve formed of a material such as silicon rubber.

反応部の一例としては、図２に示すように、流路の底面１１から流路の上面１０に向かって突起部からなるダム部９を形成し、抗体が固定化されたマイクロビーズ８を流路内に固定化したものが挙げられる。   As an example of the reaction part, as shown in FIG. 2, a dam part 9 made of a protrusion is formed from the bottom face 11 of the flow path toward the upper face 10 of the flow path, and the microbeads 8 on which the antibody is immobilized are allowed to flow. The thing fixed in the road is mentioned.

本発明のマイクロチップの他の実施態様としては、図１に示すマイクロチップにおいて、導入孔２、リザーバー部３、５、７、バルブ４１、４２、４３が順に形成され、微細な流路により分岐部がなく導通されているマイクロチップで、反応部がない形態である。
この第１のマイクロチップと、反応部を有する第２のマイクロチップとを導通手段により連結して化学反応検出システムが得られる。 As another embodiment of the microchip of the present invention, in the microchip shown in FIG. 1, an introduction hole 2, reservoir portions 3, 5, and 7, valves 41, 42, and 43 are formed in this order, and branched by a fine flow path. This is a microchip that is conductive without a part and has no reaction part.
A chemical reaction detection system is obtained by connecting the first microchip and the second microchip having the reaction part by a conduction means.

本発明のマイクロチップの導入孔からポンプを用いて送液する際に使用するチューブ、あるいはマイクロチップを連結するのに使用するチューブやジョイントの素材は、反応物の付着が少ないテフロン（登録商標）、ポリプロピレン、ＰＥＥＫ（＝ポリエーテルエーテルケトン）などが好ましい。   Teflon (registered trademark) is a tube used when liquid is fed from the introduction hole of the microchip of the present invention by using a pump, or a tube or joint material used to connect the microchip is less attached to the reactant. , Polypropylene, PEEK (= polyetheretherketone) and the like are preferable.

本発明において、化学反応を検出する検出部を、反応部を有するマイクロチップと同じチップ上に設けても良い。又は反応部を有するマイクロチップと、検出部を有する別の検出用マイクロチップとを連結する方法もある。検出機としては熱レンズ検出器（以下、ＴＬＭと略す。）のような流体を正確に測定できるようなものが好ましい。   In the present invention, the detection unit for detecting a chemical reaction may be provided on the same chip as the microchip having the reaction unit. Alternatively, there is a method of connecting a microchip having a reaction part and another detection microchip having a detection part. A detector that can accurately measure a fluid such as a thermal lens detector (hereinafter abbreviated as TLM) is preferable.

本発明の化学検出方法について、抗原抗体反応の場合の例を以下に示す。図１に示すマイクロチップのリザーバー部に標識抗体溶液、抗原溶液、及び洗浄剤充填し、各バルブを解放する事により反応を開始させる。又は、図１に示すマイクロチップのリザーバー部に標識抗体溶液、及び洗浄剤充填し、この状態でマイクロチップを凍結保存する。こうすることによって、リザーバー内の試薬の蒸発や流路内への気泡の混入を防止することができる。使用時に、マイクロチップを解凍し、抗原リザーバー部に抗原溶液を充填し各バルブを解放する事により反応を開始させる。   Regarding the chemical detection method of the present invention, an example in the case of an antigen-antibody reaction is shown below. The reservoir of the microchip shown in FIG. 1 is filled with a labeled antibody solution, an antigen solution, and a cleaning agent, and the reaction is started by opening each valve. Alternatively, the reservoir portion of the microchip shown in FIG. 1 is filled with a labeled antibody solution and a cleaning agent, and the microchip is stored frozen in this state. By doing so, it is possible to prevent evaporation of the reagent in the reservoir and mixing of bubbles into the flow path. At the time of use, the reaction is started by thawing the microchip, filling the antigen reservoir with the antigen solution, and releasing each valve.

《実施例》
図１のように導入孔２、標識抗体溶液リザーバー３、バルブ４１、洗浄剤リザーバー５、バルブ４２、抗原溶液リザーバー７、バルブ４３、反応部８がこの順に並んだ微細な流路を有し且つ、流路内にダム９を有するマイクロチップ１を用意した。マイクロチップの素材は、環状オレフィン樹脂であり、寸法は７０ｍｍ×３０ｍｍ×０．７５ｍｍである。流路の幅は０．５ｍｍ、深さは０．５ｍｍである。バルブはシリコンゴム製の３方弁を用いた。
次いで、抗CEA（＝CARCINOEMBRYONIC ANTIGEN） 抗体をポリスチレン製のマイクロビーズに固相化し、１％ウシ血清アルブミンを含むリン酸緩衝液で抗ＣＥＡ抗体により被覆されていないマイクロビーズ表面を被覆した。該マイクロビーズをマイクロチップに導入し流路内の反応部８に固定した。所定のリザーバーにCEA抗原溶液、洗浄剤(リン酸緩衝液)、ペルオキシダーゼ標識抗体溶液を封入した。その後、導入孔２より洗浄剤を２μL/分の速度で導入して、マイクロビーズに固相化された抗CEA抗体を、ＣＥＡ抗原、ペルオキシダーゼ標識抗ＣＥＡ抗体と順に反応させた。
最後に3,3',5,5'-TetramethylbenzidineとＨ2Ｏ2の混合溶液を基質として導入し酵素反応を実施した。該酵素反応により発色した基質を検出用マイクロチップ上でＴＬＭ(励起波長=６５０ｎｍ)を用いて発色量を計測した。各抗原濃度につきｎ＝５で測定しそれらの平均値及びばらつきを示す指標であるＣＶ値(=標準偏差/平均値×１００)を計算した。 "Example"
As shown in FIG. 1, the introduction hole 2, the labeled antibody solution reservoir 3, the valve 41, the cleaning agent reservoir 5, the valve 42, the antigen solution reservoir 7, the valve 43, and the reaction part 8 have fine channels arranged in this order, and A microchip 1 having a dam 9 in the flow path was prepared. The material of the microchip is a cyclic olefin resin, and the dimensions are 70 mm × 30 mm × 0.75 mm. The width of the channel is 0.5 mm and the depth is 0.5 mm. A three-way valve made of silicon rubber was used as the valve.
Next, an anti-CEA (= CARCINOEMBRYONIC ANTIGEN) antibody was immobilized on polystyrene microbeads, and a microbead surface not coated with an anti-CEA antibody was coated with a phosphate buffer containing 1% bovine serum albumin. The microbead was introduced into the microchip and fixed to the reaction section 8 in the flow path. A CEA antigen solution, a detergent (phosphate buffer), and a peroxidase-labeled antibody solution were sealed in a predetermined reservoir. Thereafter, a detergent was introduced from the introduction hole 2 at a rate of 2 μL / min, and the anti-CEA antibody immobilized on the microbeads was reacted with CEA antigen and peroxidase-labeled anti-CEA antibody in this order.
Finally, a mixed solution of 3,3 ′, 5,5′-Tetramethylbenzidine and H 2 O 2 was introduced as a substrate to carry out an enzyme reaction. The color development amount of the substrate colored by the enzyme reaction was measured on a detection microchip using TLM (excitation wavelength = 650 nm). The CV value (= standard deviation / average value × 100), which is an index showing the average value and variation of each antigen concentration measured at n = 5, was calculated.

《比較例》
図３のように導入孔２、反応部８、排出孔６が順に並んだ微細な流路を有し且つ、流路内にダム９を有するマイクロチップ１を用意した。マイクロチップの素材は、環状オレフィン樹脂であり、寸法は７０ｍｍ×３０ｍｍ×０．７５ｍｍである。流路の幅は０．５ｍｍ、深さは０．５ｍｍである。
次いで、抗CEA（＝CARCINOEMBRYONIC ANTIGEN） 抗体をポリスチレン製のマイクロビーズに固相化し、１％ウシ血清アルブミンを含むリン酸緩衝液で抗ＣＥＡ抗体により被覆されていないマイクロビーズ表面を被覆した。該マイクロビーズをマイクロチップに導入し流路内の反応部８に固定した。
CEA抗原、洗浄剤(リン酸緩衝液)、ペルオキシダーゼ標識抗体の各試薬を別々のマイクロシリンジに入れそれぞれ順に導入孔２よりマイクロチップへ導入し、実施例と同様の反応を行った。
最後に3,3',5,5'-TetramethylbenzidineとＨ2Ｏ2の混合溶液を基質として導入し酵素反応を実施した。該酵素反応により発色した基質を検出用マイクロチップ上でＴＬＭ(励起波長=６５０ｎｍ)を用いて発色量を計測した。各抗原濃度につきｎ＝５で測定しそれらの平均値及びばらつきを示す指標であるＣＶ値(=標準偏差/平均値×１００)を計算した。 《Comparative example》
As shown in FIG. 3, a microchip 1 having a fine flow path in which an introduction hole 2, a reaction portion 8, and a discharge hole 6 are arranged in this order and having a dam 9 in the flow path was prepared. The material of the microchip is a cyclic olefin resin, and the dimensions are 70 mm × 30 mm × 0.75 mm. The width of the channel is 0.5 mm and the depth is 0.5 mm.
Next, an anti-CEA (= CARCINOEMBRYONIC ANTIGEN) antibody was immobilized on polystyrene microbeads, and a microbead surface not coated with an anti-CEA antibody was coated with a phosphate buffer containing 1% bovine serum albumin. The microbead was introduced into the microchip and fixed to the reaction section 8 in the flow path.
Each reagent of CEA antigen, detergent (phosphate buffer) and peroxidase-labeled antibody was put in a separate microsyringe and introduced into the microchip through the introduction hole 2 in order, and the same reaction as in the example was performed.
Finally, a mixed solution of 3,3 ′, 5,5′-Tetramethylbenzidine and H 2 O 2 was introduced as a substrate to carry out an enzyme reaction. The color development amount of the substrate colored by the enzyme reaction was measured on a detection microchip using TLM (excitation wavelength = 650 nm). The CV value (= standard deviation / average value × 100), which is an index showing the average value and variation of each antigen concentration measured at n = 5, was calculated.

実施例及び比較例の抗原濃度に応じた発色量（＝ＴＬＭシグナル）を計測した結果を表１に示した。実施例、比較例ともにほぼ同等なTLMシグナル（平均値）を示したがＣＶ値は実施例の方が１／３に低減した。   Table 1 shows the results of measuring the color development amount (= TLM signal) according to the antigen concentrations of the examples and comparative examples. The TLM signal (average value) was almost the same in both Examples and Comparative Examples, but the CV value was reduced to 1/3 in the Examples.

実施例で使用したマイクロチップMicrochip used in the examples 流路内の反応部のダム部（断面図）Dam part of the reaction part in the flow path (cross section) 比較例で使用したマイクロチップMicrochip used in the comparative example

符号の説明Explanation of symbols

１ マイクロチップ
２ 導入孔
３．標識抗体溶液のリザーバー
４１、４２、４３ 流路切換バルブ
５ 洗浄剤リザーバー
６ 排出孔
７ 抗原溶液リザーバー
８ 反応部（抗体が固定化されたマイクロビーズ）
９ ダム 1 Microchip 2 Introduction hole 3. Reservoir 41, 42, 43 of labeled antibody solution Channel switching valve 5 Cleaning agent reservoir 6 Discharge hole 7 Antigen solution reservoir 8 Reaction part (microbead on which antibody is immobilized)
9 Dam

Claims (6)

幅が１μｍ〜１ｃｍの流路、前記流路に流体を導入可能な導入孔、及び前記流路から流体を排出可能な排出孔とを有するマイクロチップであって、更に化学反応又は生化学反応に使用する試薬を蓄えるためのリザーバー部を同一チップ内に有し、かつ前記流路によって導通していることを特徴とするマイクロチップ。 A microchip having a flow path having a width of 1 μm to 1 cm, an introduction hole capable of introducing a fluid into the flow path, and a discharge hole capable of discharging the fluid from the flow path, and further for chemical reaction or biochemical reaction A microchip having a reservoir portion for storing a reagent to be used in the same chip and being connected by the flow path. 化学反応又は生化学反応を目的とした反応部、幅が１μｍ〜１ｃｍの流路、前記流路に流体を導入可能な導入孔、及び前記流路から流体を排出可能な排出孔を有するマイクロチップであって、更に前記反応に使用する試薬を蓄えるためのリザーバー部を同一チップ内に有し、導入孔、リザーバー部、反応部がこの順に配置され、かつ前記流路によって導通していることを特徴とするマイクロチップ。 A microchip having a reaction part intended for a chemical reaction or biochemical reaction, a channel having a width of 1 μm to 1 cm, an introduction hole through which a fluid can be introduced into the channel, and a discharge hole through which the fluid can be discharged from the channel And having a reservoir part for storing the reagent used for the reaction in the same chip, the introduction hole, the reservoir part, and the reaction part being arranged in this order, and being connected by the flow path. A featured microchip. 前記リザーバー部と前記反応部との間に更に流体の流量の調節又は流体の送液／停止を切り替えることができるバルブを有する請求項２記載のマイクロチップ。 3. The microchip according to claim 2, further comprising a valve capable of switching a flow rate of a fluid or switching a fluid supply / stop between the reservoir unit and the reaction unit. 前記流路が分岐部がないものであり、前記流路内にリザーバー部及び反応部が形成されている請求項２又は３記載のマイクロチップ。 4. The microchip according to claim 2, wherein the flow path has no branch part, and a reservoir part and a reaction part are formed in the flow path. 請求項１記載のマイクロチップと、化学反応又は生化学反応を目的とした反応部、幅が１μｍ〜１ｃｍの流路、前記流路に流体を導入可能な導入孔、及び前記流路から流体を排出可能な排出孔を有する第２のマイクロチップとを導通手段により連結して構成される化学反応検出システム。 The microchip according to claim 1, a reaction part intended for a chemical reaction or a biochemical reaction, a channel having a width of 1 μm to 1 cm, an introduction hole capable of introducing a fluid into the channel, and a fluid from the channel A chemical reaction detection system configured by connecting a second microchip having a discharge hole that can be discharged by a conduction means. 請求項１〜４いずれか記載のマイクロチップに化学反応又は生化学反応に使用する試薬を導入し、使用するまでは凍結保存し、使用直前に解凍する事によって化学反応又は生化学反応を行う事を特徴とする化学反応検出方法。 A reagent used for a chemical reaction or biochemical reaction is introduced into the microchip according to any one of claims 1 to 4, and the chemical reaction or biochemical reaction is performed by refrigeration until use and thawing immediately before use. A chemical reaction detection method characterized by the above.
JP2006118691A 2006-04-24 2006-04-24 Microchip and system for detecting chemical reaction Pending JP2007292527A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2006118691A JP2007292527A (en) 2006-04-24 2006-04-24 Microchip and system for detecting chemical reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2006118691A JP2007292527A (en) 2006-04-24 2006-04-24 Microchip and system for detecting chemical reaction

Publications (1)

Publication Number Publication Date
JP2007292527A true JP2007292527A (en) 2007-11-08

Family

ID=38763280

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006118691A Pending JP2007292527A (en) 2006-04-24 2006-04-24 Microchip and system for detecting chemical reaction

Country Status (1)

Country Link
JP (1) JP2007292527A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010082279A1 (en) 2009-01-15 2010-07-22 パナソニック株式会社 Flow channel structure and method for manufacturing same
JP2013106595A (en) * 2011-11-24 2013-06-06 Institute Of Microchemical Technology Micro device and bioassay system
JP2015142893A (en) * 2014-01-31 2015-08-06 株式会社日立製作所 System and method for providing drugs

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002236131A (en) * 2000-12-08 2002-08-23 Minolta Co Ltd Microchip
JP2004138583A (en) * 2002-10-21 2004-05-13 Sumitomo Bakelite Co Ltd Micro-chip for measuring function of cell
JP2005065607A (en) * 2003-08-26 2005-03-17 Hitachi Ltd Gene treating chip and gene treating apparatus
JP2005083510A (en) * 2003-09-09 2005-03-31 Toshiba Corp Valve device, chemical analysis device and chemical analysis system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002236131A (en) * 2000-12-08 2002-08-23 Minolta Co Ltd Microchip
JP2004138583A (en) * 2002-10-21 2004-05-13 Sumitomo Bakelite Co Ltd Micro-chip for measuring function of cell
JP2005065607A (en) * 2003-08-26 2005-03-17 Hitachi Ltd Gene treating chip and gene treating apparatus
JP2005083510A (en) * 2003-09-09 2005-03-31 Toshiba Corp Valve device, chemical analysis device and chemical analysis system

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010082279A1 (en) 2009-01-15 2010-07-22 パナソニック株式会社 Flow channel structure and method for manufacturing same
JPWO2010082279A1 (en) * 2009-01-15 2012-06-28 パナソニック株式会社 Channel structure and manufacturing method thereof
US8840850B2 (en) 2009-01-15 2014-09-23 Panasonic Corporation Flow channel structure and method of manufacturing same
JP5678272B2 (en) * 2009-01-15 2015-02-25 パナソニックＩｐマネジメント株式会社 Channel structure and manufacturing method thereof
JP2013106595A (en) * 2011-11-24 2013-06-06 Institute Of Microchemical Technology Micro device and bioassay system
JP2015142893A (en) * 2014-01-31 2015-08-06 株式会社日立製作所 System and method for providing drugs
US10005060B2 (en) 2014-01-31 2018-06-26 Hitachi, Ltd. Drug provision system and drug provision method

Similar Documents

Publication Publication Date Title
JP4754394B2 (en) Microchip
US20200094252A1 (en) Microfluidic chip for separating and detecting whole blood sample and detection method thereof
CN105026932B (en) Micro-fluidic distributing equipment
JP2007017354A (en) Chemical reaction detecting system
US7820109B2 (en) Testing chip and micro analysis system
US20120052557A1 (en) Microchip
WO2001013127A1 (en) Analyzing cartridge and liquid feed control device
US20090232708A1 (en) Microchip
CN101754812A (en) Fluid connector and microfluid system
JP5902426B2 (en) Liquid feeding device and liquid feeding method
JP2007051881A (en) Reaction cartridge, reaction apparatus, and moving method for solution of the reaction cartridge
JP5137012B2 (en) Microchip
JP2007155491A (en) Micro reactor system and liquid feed method
JP2007010341A (en) Immunity analysis method
RU2725264C2 (en) Fluid analysis system
WO2017213123A1 (en) Fluid device
CN106660040A (en) Rotatable cartridge for processing and analyzing a biological sample
JP2009139369A (en) Microchip
WO2009113010A1 (en) A sensor device and a method of detecting compounds, particles or complexes
JP2007292527A (en) Microchip and system for detecting chemical reaction
JP4732913B2 (en) Microchip and method of using microchip
JP5177533B2 (en) Microchip
US20090291025A1 (en) Microchip And Method Of Using The Same
EP1264637B1 (en) Laboratory microchip device and method for chemical analysis
US9493812B2 (en) Method for detecting a target analyte that exhibits protease enzyme activity

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20081110

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20101202

A131 Notification of reasons for refusal

Effective date: 20101214

Free format text: JAPANESE INTERMEDIATE CODE: A131

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20110412