JP2007289160A - Lactic fermentation drink of sugar cane and method for preparation of the same - Google Patents
Lactic fermentation drink of sugar cane and method for preparation of the same Download PDFInfo
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Abstract
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本発明は、サトウキビ搾汁液または黒糖と乳原料等を含有する培地に、乳酸菌を接種して乳酸発酵させることにより得られるサトウキビ乳酸発酵飲料およびその製造方法に関する。 TECHNICAL FIELD The present invention relates to a sugarcane lactic acid fermented beverage obtained by inoculating lactic acid bacteria into a medium containing sugarcane juice or brown sugar and milk raw materials and fermenting lactic acid, and a method for producing the same.
沖縄・南西諸島の基幹作物であるサトウキビは主に製糖原料として利用されているが、近年は収益性が停滞し、作付面積も年々減少している。それ故、サトウキビの多用途開発は、資源の有効利用および環境負荷軽減の観点からも危急の問題である。 Sugarcane, a key crop in Okinawa and the Nansei Islands, is mainly used as a raw material for sugar production, but in recent years profitability has stagnated and the acreage has decreased year by year. Therefore, multi-use development of sugarcane is an urgent problem from the viewpoint of effective use of resources and reduction of environmental load.
サトウキビにはポリフェノールが多く含まれており、その加工製品である黒糖においてはポリフェノールや抗酸化能についての研究が多くなされている。しかし、ポリフェノール自身が渋味や苦味の原因となることなどから、また、飲料への利用はほとんど研究されておらず、わずかに数件が知られているに過ぎない（特許文献１〜３）。 Sugarcane contains a lot of polyphenols, and many researches on polyphenols and antioxidant capacity have been conducted on brown sugar, which is a processed product. However, since polyphenol itself causes astringency and bitterness, the use in beverages is hardly studied, and only a few cases are known (Patent Documents 1 to 3). .
また、サトウキビには血圧降下作用が期待できるγ−アミノ酪酸（ＧＡＢＡ）が含まれていることも明らかになっているが、上記のような問題点からサトウキビの飲料への利用は進んでいないのが実情であった。
従って、本発明は、サトウキビを飲料として摂取でき、サトウキビ由来のポリフェノールや抗酸化能を活かし、かつ、血圧降下作用が期待できるＧＡＢＡを豊富に含んだ飲料を提供することを課題とするものである。 Accordingly, an object of the present invention is to provide a beverage that is rich in GABA that can ingest sugarcane as a beverage, that makes use of sugarcane-derived polyphenols and antioxidant activity, and that can be expected to have a blood pressure lowering effect. .
本発明者らは、上記飲料を得るために種々検討を行ったところ、サトウキビの搾汁液または黒糖と乳原料とを含有する培地で、乳酸菌を乳酸発酵させることにより得られる乳酸発酵飲料が、風味良好であることを見出した。また、乳酸発酵に用いる乳酸菌として特定のものを用いると、ＧＡＢＡ含量が特に高くなることを見出した。更に、これらの乳酸発酵において、サトウキビ由来のポリフェノールや抗酸化能が影響を受けにくいことを見出した。 The present inventors have made various studies to obtain the above beverage. As a result, a lactic acid fermented beverage obtained by subjecting lactic acid bacteria to lactic acid fermentation in a medium containing sugarcane juice or brown sugar and a milk raw material has a flavor. It was found to be good. Moreover, when a specific thing was used as lactic acid bacteria used for lactic acid fermentation, it discovered that GABA content became high especially. Furthermore, in these lactic acid fermentation, it discovered that the polyphenol derived from sugarcane and antioxidant ability were hard to be influenced.
すなわち本発明は、サトウキビ搾汁液または黒糖と乳原料とを含有する培地に、乳酸菌を接種し、次いで、乳酸発酵させることにより得られるサトウキビ乳酸発酵飲料である。 That is, the present invention is a sugarcane lactic acid fermented beverage obtained by inoculating a lactic acid bacterium into a medium containing sugarcane juice or brown sugar and a milk raw material, followed by lactic acid fermentation.
また、本発明は、サトウキビ搾汁液または黒糖と乳原料とを含有する培地に、乳酸菌を接種し、次いで、乳酸発酵させることを特徴とするサトウキビ乳酸発酵飲料の製造方法である。 Moreover, this invention is a manufacturing method of the sugarcane lactic acid fermented drink characterized by inoculating the culture medium containing sugarcane juice or brown sugar, and a milk raw material, and then fermenting lactic acid.
本発明のサトウキビ乳酸発酵飲料は、サトウキビを原料とした新しい飲料であり、ポリフェノール含有量、抗酸化能が高められたものである。これに加えてＧＡＢＡ含有量がサトウキビに元来含まれている量に乳酸菌が産生することによって増強されたものである。 The sugarcane lactic acid fermented beverage of the present invention is a new beverage made from sugarcane, and has enhanced polyphenol content and antioxidant capacity. In addition to this, the GABA content is enhanced by the production of lactic acid bacteria to the amount originally contained in sugarcane.
従って、本発明のサトウキビ乳酸発酵飲料は乳酸発酵飲料による健康増進効果に併せてＧＡＢＡによる血圧降下作用が見込まれることから、健康志向の飲料としての提供が可能であり、サトウキビの用途拡大に資するものである。 Therefore, since the sugarcane lactic acid fermented beverage of the present invention is expected to have a blood pressure lowering effect by GABA in addition to the health promoting effect of the lactic acid fermented beverage, it can be provided as a health-oriented beverage and contributes to the expansion of sugarcane applications. It is.
本発明のサトウキビ乳酸発酵飲料（以下、「本発明飲料」という）は、サトウキビ搾汁液または黒糖と乳原料とを含有する培地に添加する以外は、従来から知られている培地や培養条件で、乳酸菌を乳酸発酵させることにより得られる。 The sugarcane lactic acid fermented beverage of the present invention (hereinafter referred to as “the beverage of the present invention”) is a conventionally known medium or culture condition, except that it is added to a medium containing sugarcane juice or brown sugar and milk raw material. It can be obtained by lactic acid fermentation of lactic acid bacteria.
培地に添加されるサトウキビ搾汁液または黒糖は、サトウキビ（学名：Saccharum officinarum）を原料とするものである。このうちサトウキビ搾汁液はサトウキビの全茎または内実部を搾汁することにより得られるものである。搾汁の手段としては特に制限されないが、例えば、ミル、スクリュープレス、ケーンセパレーション等が挙げられる。前記搾汁の手段により得られる搾汁液は、加熱殺菌処理や遠心分離を行って不溶性成分を除去したものであってもよく、更にこれを常法により濃縮したものであってもよい。また、黒糖は、前記サトウキビ搾汁液をそのまま煮詰めて固体化させたものである。なお、本発明飲料の製造においてはＧＡＢＡ等の含有量の点および作業工程の簡略化の点からサトウキビ搾汁液を培地に添加することが好ましい。 Sugarcane juice or brown sugar added to the medium is made from sugarcane (scientific name: Saccharum officinarum). Among them, the sugar cane juice is obtained by squeezing the whole stem or inner solid part of sugar cane. Although it does not restrict | limit especially as a means of squeezing, For example, a mill, a screw press, a cane separation, etc. are mentioned. The squeezed liquid obtained by the squeezing means may be one obtained by removing heat-insoluble components by heat sterilization treatment or centrifugation, and may be one obtained by concentrating the squeezed liquid by a conventional method. Brown sugar is obtained by boiling the sugar cane juice as it is and solidifying it. In addition, in manufacture of this invention drink, it is preferable to add a sugarcane juice to a culture medium from the point of content, such as GABA, and the point of simplification of an operation | work process.
また、培地にサトウキビ搾汁液または黒糖と共に添加される乳原料としては、牛乳、山羊乳、羊乳等の全乳、脱脂乳または脱脂粉乳等が挙げられる。 Examples of the milk raw material added to the medium together with sugarcane juice or brown sugar include whole milk such as cow's milk, goat's milk, and sheep milk, skim milk, and skim milk powder.
上記サトウキビ搾汁液と乳原料とを含有する培地としては、例えば、水５０重量部に、サトウキビ搾汁液（Ｂｒｉｘ２２程度のもの）１０〜５０重量部、好ましくは３０重量部程度および乳原料を５〜１０重量部、好ましくは１０重量部程度溶解して加熱殺菌したものが挙げられる。 Examples of the medium containing the sugar cane juice and milk raw material include 10 to 50 parts by weight, preferably about 30 parts by weight and 5 to 5 parts by weight of sugar cane juice (of Brix 22) in 50 parts by weight of water. 10 parts by weight, preferably about 10 parts by weight dissolved and heat sterilized.
また、上記黒糖と乳原料とを含有する培地としては、例えば、水５０重量部に、黒糖（Ｂｒｉｘ８０〜８８程度のもの）１.５〜９重量部、好ましくは５重量部程度および乳原料を５〜１０重量部、好ましくは１０重量部程度溶解して加熱殺菌したものが挙げられる。 Moreover, as a culture medium containing the said brown sugar and milk raw material, 1.5 to 9 weight part of brown sugar (thing of about Brix80-88), Preferably about 5 weight part and milk raw material are added to 50 weight part of water, for example. 5-10 parts by weight, preferably about 10 parts by weight dissolved and heat sterilized.
更に、この培地には上記サトウキビ搾汁液または黒糖と乳原料以外に、砂糖、ビタミンＣ等の公知の成分を添加することができる。 Furthermore, in addition to the sugarcane juice or brown sugar and the milk material, known ingredients such as sugar and vitamin C can be added to this medium.
上記培地に接種される乳酸菌としては、特に限定されないが、例えば、ラクトバチルス属、ラクトコッカス属、ストレプトコッカス属、エンテロコッカス属等の乳酸菌を使用することができる。これら乳酸菌の中でも、培地中のＧＡＢＡを分解することなく、グルタミン酸を資化してＧＡＢＡを産生する乳酸菌（以下、これを「ＧＡＢＡ産生乳酸菌」という）が好ましい。このような乳酸菌としては、例えば、ラクトコッカス・ラクチス・サブスピーシーズ・ラクチス（Lactococcus lactis subsp. lactis）、ラクトコッカス・ラクティス・バイオバラエティ・ディアセチラクティス（Lactococcus lactis biovar. diacetylactis）、ラクトコッカス・ラクティス（Lactococcus Lactis）、ラクトバチルス・ブレビス（Lactobacillus brevis）、ラクトバチルス・ヒルガルディー（Lactobacillus hilgardii）、ラクトバチルス・ケフィリ（Lactobacillus kefiri）、ラクトバチルス・プランタラム（Lactobacillus plantarum）、ストレプトコッカス・サーモフィルス（Streptococcus thermophilus）、エンテロコッカス・カセリフラバス（Enterococcus casseliflavus）等が好ましい。これらＧＡＢＡ産生乳酸菌の中でも、特に、ラクトコッカス・ラクチス・サブスピーシーズ・ラクチスまたはラクトコッカス・ラクティス・バイオバラエティ・ディアセチラクティスは最終的に得られる飲料の風味が良好であるので好ましい。 Although it does not specifically limit as lactic acid bacteria inoculated on the said culture medium, For example, lactic acid bacteria, such as Lactobacillus genus, Lactococcus genus, Streptococcus genus, Enterococcus genus, can be used. Among these lactic acid bacteria, lactic acid bacteria that assimilate glutamic acid and produce GABA without degrading GABA in the medium (hereinafter referred to as “GABA-producing lactic acid bacteria”) are preferable. Examples of such lactic acid bacteria include Lactococcus lactis subsp. Lactis, Lactococcus lactis biovariate diacetylactis, Lactococcus lactis biovar. Diacetylactis (Lactococcus lactis biovar. Diacetylactis) Lactococcus Lactis, Lactobacillus brevis, Lactobacillus hilgardii, Lactobacillus kefiri, Lactobacillus plantarum, Streptococcus thermo cus octo Enterococcus casseliflavus and the like are preferable. Among these GABA-producing lactic acid bacteria, Lactococcus lactis subspecies lactis or Lactococcus lactis biovariety diacetactis is preferable because the beverage finally obtained has a good flavor.
また、本発明においては、上記ＧＡＢＡ産生乳酸菌と共に原料中のグルタミン酸を遊離させる乳酸菌（以下、これを「グルタミン酸遊離乳酸菌」という）を培地に接種するとＧＡＢＡの産生量が相乗的に増加するので好ましい。このようなグルタミン酸遊離乳酸菌としては、例えば、ラクトバチルス・アシドフィルス（Lactobacillus acidophilus）、ラクトバチルス・デルブルエッキー・サブスピーシーズ・ブルガリカス（Lactobacillus delbruekii subsp. bulgaricus）、ラクトバチルス・デルブルッキー・サブスピーシーズ・ラクチス（Lactobacillus delbruekii subsp. lactis）、ラクトバチルス・ヘルベチカス（Lactobacillus helveticus）、ラクトバチルス・ゼアエ（Lactobacillus zeae）等が挙げられる。これらグルタミン酸遊離乳酸菌の中でもラクトバチルス・ゼアエは最終的に得られる飲料の風味が良好であるので好ましい。上記ＧＡＢＡ産生乳酸菌とグルタミン酸遊離乳酸菌を共に培地に接種する際の比率は容量比で、４：１〜１：４、好ましくは１：１程度である。 In the present invention, it is preferable to inoculate the medium with lactic acid bacteria that liberate glutamic acid in the raw material together with the GABA-producing lactic acid bacteria (hereinafter referred to as “glutamic acid-free lactic acid bacteria”) because the amount of GABA production increases synergistically. Examples of such glutamate-free lactic acid bacteria include Lactobacillus acidophilus, Lactobacillus delbruekii subspices bulgaricus, Lactobacillus delbruekii subsp. Bulgaricus, Lactobacillus delbruekii subspices lactis. (Lactobacillus delbruekii subsp. Lactis), Lactobacillus helveticus, Lactobacillus zeae and the like. Among these glutamic acid-free lactic acid bacteria, Lactobacillus zeae is preferable because the beverage finally obtained has a good flavor. The ratio of inoculating the GABA-producing lactic acid bacterium and glutamic acid-free lactic acid bacterium together in the medium is a volume ratio of 4: 1 to 1: 4, preferably about 1: 1.
上記したＧＡＢＡ産生乳酸菌およびグルタミン酸遊離乳酸菌の組み合わせの一例としては、ストレプトコッカス・サーモフィルス、ラクトバチルス・デルブルッキー・サブスピーシーズ・ブルガリクスおよびラクトバチルス・デルブルッキー・サブスピーシーズ・ラクチスの組み合わせが挙げられる。この組み合わせのものはＣＨＲ−ＨＡＮＳＥＮ社からＦＤ−ＤＶＳ ＹＣ−１８０−Ｙｏ−Ｆｌｅｘ（登録商標）として市販されているので、これを利用しても良い。 As an example of the combination of the above-mentioned GABA-producing lactic acid bacteria and glutamic acid-free lactic acid bacteria, there is a combination of Streptococcus thermophilus, Lactobacillus delbrucky subspecies bulgaricus and Lactobacillus delbrucky subspecies lactis. Since this combination is commercially available from CHR-HANSEN as FD-DVS YC-180-Yo-Flex (registered trademark), it may be used.
なお、上記したＧＡＢＡ産生乳酸菌およびグルタミン酸遊離乳酸菌のいずれも生物遺伝資源センター、日本乳業技術協会等より入手可能なものである。 Note that both the GABA-producing lactic acid bacterium and the glutamic acid-free lactic acid bacterium described above are available from the Biological Genetic Resource Center, the Japan Dairy Industry Association, etc.
本発明飲料の製造は、上記乳酸菌を常法によりスターターとし、これをサトウキビ搾汁液または黒糖と乳原料とを含有する培地に対して１０〜４０重量部、好ましくは１０重量部程度接種し、乳酸発酵させることにより行われる。乳酸発酵の条件としては、２５℃〜３５℃程度で４８〜６０時間、好ましくは６０時間程度で、ｐＨ３.８〜５.０程度、好ましくはｐＨ４.４程度まで発酵させる条件が挙げられる。また、乳酸菌としてＧＡＢＡ産生乳酸菌とグルタミン酸遊離乳酸菌とを併用する場合の乳酸発酵の条件としては、２５℃〜３５℃程度で３６〜６０時間、好ましくは４８時間程度で、ｐＨ３.８〜５.０程度、好ましくはｐＨ４.４程度まで発酵させる条件が挙げられる。 The beverage of the present invention is produced by using the above lactic acid bacteria as a starter by a conventional method, inoculating 10 to 40 parts by weight, preferably about 10 parts by weight, with respect to a medium containing sugarcane juice or brown sugar and milk ingredients. This is done by fermenting. The conditions for lactic acid fermentation include conditions of fermentation at about 25 ° C. to 35 ° C. for 48 to 60 hours, preferably about 60 hours, and about pH 3.8 to 5.0, preferably about pH 4.4. Moreover, as conditions for lactic acid fermentation when GABA-producing lactic acid bacteria and glutamic acid-free lactic acid bacteria are used in combination as lactic acid bacteria, the conditions are about 25 to 35 ° C. for 36 to 60 hours, preferably about 48 hours, and pH 3.8 to 5.0. The conditions for fermentation to a degree, preferably about pH 4.4.
本発明飲料の製造方法の好ましい、一態様を挙げれば次の通りである。まず、サトウキビをミル搾汁し、搾汁液を得、その搾汁液を加熱殺菌し、不溶性成分をろ別してサトウキビ搾汁液を調製する。次に、１０重量部の脱脂粉乳と５０重量部の水とを混合後、加熱殺菌し、これとサトウキビ搾汁液とを混合して培地を調製する。更に、この培地に乳酸菌スターターを１０重量部接種した後、３０℃で６０時間、ｐＨが４.４となるまで乳酸発酵させる。 A preferred embodiment of the method for producing the beverage of the present invention is as follows. First, sugar cane is squeezed into a mill to obtain a squeezed liquid, the squeezed liquid is sterilized by heating, and insoluble components are filtered off to prepare a sugar cane squeezed liquid. Next, 10 parts by weight of skim milk powder and 50 parts by weight of water are mixed and then sterilized by heating, and this and sugarcane juice are mixed to prepare a medium. Furthermore, after inoculating 10 parts by weight of lactic acid bacteria starter into this medium, lactic acid fermentation is carried out at 30 ° C. for 60 hours until pH becomes 4.4.
本発明飲料には、本発明の効果を損なわない程度に、香料、砂糖、果汁、ビタミンＣ等を配合しても良い。 You may mix | blend a fragrance | flavor, sugar, fruit juice, vitamin C, etc. with this invention drink to such an extent that the effect of this invention is not impaired.
斯くして得られる本発明飲料は、１００ｍｌあたりＧＡＢＡを２ｍｇ以上、好ましくは６ｍｇ以上含有するものである。また、この飲料は１００ｍｌあたりＤＰＰＨラジカルラジカル消去活性が２０μＭ以上、好ましくは５０μＭ以上である。更に、この飲料は１００ｍｌあたりポリフェノールを１０ｍｇ以上、好ましくは１５ｍｇ以上含有するものである。 The beverage of the present invention thus obtained contains 2 mg or more, preferably 6 mg or more of GABA per 100 ml. Further, this beverage has a DPPH radical radical scavenging activity of 20 μM or more, preferably 50 μM or more per 100 ml. Further, this beverage contains 10 mg or more, preferably 15 mg or more of polyphenol per 100 ml.
本発明飲料は、サトウキビ由来のポリフェノールや抗酸化能を活かしたものであり、かつ、血圧降下作用が期待できるＧＡＢＡを豊富に含んでいるので健康志向の飲料として優れたものとなる。 The beverage of the present invention makes use of sugarcane-derived polyphenols and antioxidant ability, and since it contains abundant GABA that can be expected to lower blood pressure, it is excellent as a health-oriented beverage.
以下、実施例を挙げ、本発明を更に詳しく説明するが、本発明はこれら実施例に何ら制約されるものではない。 EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in more detail, this invention is not restrict | limited at all by these Examples.
実 施 例 １
サトウキビ乳酸発酵飲料の製造（１）：
まず、サトウキビ（農林８号)をミル搾汁し、加熱殺菌処理を行った後に遠心分離により不溶性成分を除去して、１００ｍｌあたりＧＡＢＡ含有量６.８ｍｇ、グルタミン酸含有量１.１ｍｇ、Ｂｒｉｘ２２のサトウキビ搾汁液を得た。次に、市販の脱脂粉乳（全酪連製）２０ｇを水８０ｇに溶解して加熱滅菌した後に、別に加熱殺菌をしたサトウキビ搾汁液１００ｍｌを加え、あらかじめ培養した乳酸菌スターター２０ｍｌを添加し、３０℃で６０時間培養して、ヨーグルト様のサトウキビ乳酸発酵飲料を得た。乳酸菌のスターターとしては、以下の表１に記載の１１種類を使用した。また、サトウキビ搾汁液を加えない培地を用いた以外は、同様の条件で調製した乳酸発酵飲料を対照とした。
Example 1
Manufacture of sugarcane lactic acid fermented beverage (1):
First, sugarcane (Agricultural Forestry No. 8) is squeezed into a mill and heat-sterilized, and then insoluble components are removed by centrifugation. A GABA content of 6.8 mg per 100 ml, a glutamic acid content of 1.1 mg, and a Brix22 sugarcane A juice was obtained. Next, after dissolving 20 g of commercially available skim milk powder (manufactured by All Dairyen) in 80 g of water and heat sterilizing, 100 ml of sugarcane juice obtained by heat sterilization is added, 20 ml of lactic acid bacteria starter cultured in advance is added, and 60 ° C. at 60 ° C. By culturing for a time, a yogurt-like sugarcane lactic acid fermented beverage was obtained. Eleven kinds of lactic acid bacteria listed in Table 1 below were used. Moreover, the lactic acid fermented drink prepared on the same conditions was used as the control except having used the culture medium which does not add sugarcane juice.
各乳酸発酵飲料のＧＡＢＡ含有量を島津アミノ酸分析システムＮａ型によるＨＰＬＣ法により測定した。また、各乳酸発酵飲料のポリフェノール含有量をポリフェノール含有量測定法（津志田藤二郎著、「食品機能分析法」、３１８、光琳、２０００）により測定し、没食子酸相当量で表した。更に、各乳酸発酵飲料の抗酸化能をＤＰＰＨ（１,１−Ｄｉｐｈｅｎｙｌ−２−ｐｉｃｒｙｌｈｙｄｒａｚｏｌ）を用いたラジカル捕捉法（須田郁夫著、「食品の機能性マニュアル集」、１６、農林水産技術会議・食品総合研究所編、１９９９）で測定し、抗酸化物質であるトロロックス（Ｔｒｏｌｏｘ）相当量で表した。これらの結果を表１に示した。 The GABA content of each lactic acid fermented beverage was measured by the HPLC method using Shimadzu amino acid analysis system Na type. Moreover, the polyphenol content of each lactic acid fermented beverage was measured by a polyphenol content measurement method (Toshida Tojiro, “Food Function Analysis Method”, 318, Korin, 2000), and expressed in terms of gallic acid equivalent. Furthermore, the antioxidant capacity of each lactic acid fermented beverage was measured by radical scavenging method using DPPH (1,1-Diphenyl-2-picrylhydrazol) (Suda Ikuo, “Food Functionality Manual Collection”, 16, Agriculture, Forestry and Fisheries Technology Conference, Measured by the National Food Research Institute, 1999), and expressed in an amount equivalent to the antioxidant Trolox. These results are shown in Table 1.
また、各乳酸発酵飲料の風味を２０〜５０才台の男女９人により下記評価基準の５段階で評価した。その結果も併せて表１に示した。 Moreover, the flavor of each lactic acid fermented drink was evaluated in five stages of the following evaluation criteria by nine men and women in the 20 to 50-year-old range. The results are also shown in Table 1.
＜風味評価基準＞
（評価） （内容）
５ ： 酸味と甘味のバランスが良く、美味しく感じ、後味も良い
４ ： 酸味と甘味のバランスが良く、美味しく感じる
３ ： 酸味と甘味のバランスが良い
２ ： 酸味と甘味のバランスが悪い
１ ： 酸味と甘味のバランスが悪く、美味しく感じない
<Flavor evaluation criteria>
(Evaluation) (Content)
5: Good balance between acidity and sweetness, good taste, good aftertaste 4: Good balance between acidity and sweetness, good taste 3: Good balance between acidity and sweetness 2: Bad balance between acidity and sweetness 1: Acidity and sweetness The balance of sweetness is bad and does not feel delicious
その結果、スターターにラクトコッカス・ラクチス・サブスピーシーズ・ラクチスおよびラクトコッカス・ラクティス・バイオバラエティ・ディアセチラクティスを用いると飲料中にＧＡＢＡが多く蓄積されることが分かった。また、サトウキビ乳酸発酵飲料においては、スターターにラクトコッカス・ラクチス・サブスピーシーズ・ラクチスを用いた場合が、ＧＡＢＡ含有量が一番多かった。また、いずれの乳酸菌をスターターに用いた場合でも、サトウキビ搾汁液の添加によってポリフェノール含有量、抗酸化能共に上昇する事がわかった。更に、ラクトコッカス・ラクチス・サブスピーシーズ・ラクチスおよびラクトコッカス・ラクティス・バイオバラエティ・ディアセチラクティスを用いると、サトウキビの風味が残存し、サトウキビ由来の甘味と乳酸由来の酸味に優れたものとなることが分かった。 As a result, it was found that when Lactococcus lactis subspecies lactis and Lactococcus lactis biovariety diacety lactis were used as a starter, a large amount of GABA was accumulated in the beverage. In addition, in sugarcane lactic acid fermented beverages, the GABA content was highest when Lactococcus / Lactis / Subspecies / Lactis was used as a starter. Moreover, even if any lactic acid bacteria were used for a starter, it turned out that both polyphenol content and antioxidant ability rise by addition of sugarcane juice. Furthermore, when Lactococcus lactis sub-species lactis and Lactococcus lactis biovariety diacetactis are used, the sugarcane flavor remains and the sugarcane-derived sweetness and lactic acid-derived acidity are excellent. I understood.
実 施 例 ２
サトウキビ乳酸発酵飲料の製造（２）：
実施例１の乳酸菌スターターにラクトバチルス・ゼアエ（Lactobacillus zeae）を各種混合比率で混合して実施例１と同様に乳酸発酵を行い、サトウキビ乳酸発酵飲料を得た。サトウキビ乳酸発酵飲料中のＧＡＢＡ含有量は１００ｍｌあたり最大１８ｍｇ以上に増加し、同様にポリフェノール含有量もサトウキビ搾汁液を添加していない乳酸発酵飲料よりも増加した。また、抗酸化能もサトウキビ搾汁液を添加していない乳酸発酵飲料よりも上昇していた。ＧＡＢＡ含有量及び官能評価より、培養時間は４８時間が良好であった。また、両菌株の混合比は容量比で１：４から４：１が好ましく、特に１：１が好ましかった。更に、上記混合比において飲料中のＧＡＢＡ含有量に顕著な差異は生じなかった。
Example 2
Manufacture of sugarcane lactic acid fermented beverage (2):
Lactobacillus zeae was mixed with the lactic acid bacteria starter of Example 1 at various mixing ratios and lactic acid fermentation was performed in the same manner as in Example 1 to obtain a sugarcane lactic acid fermented beverage. The GABA content in the sugar cane lactic acid fermented beverage increased to a maximum of 18 mg or more per 100 ml, and the polyphenol content also increased as compared to the lactic acid fermented beverage to which no sugar cane juice was added. In addition, the antioxidant capacity was also higher than that of lactic acid fermented beverages without added sugarcane juice. From the GABA content and sensory evaluation, the culture time was good for 48 hours. The mixing ratio of both strains is preferably from 1: 4 to 4: 1 by volume, and particularly preferably 1: 1. Furthermore, there was no significant difference in GABA content in the beverage at the above mixing ratio.
実 施 例 ３
サトウキビ乳酸発酵飲料の製造（３）：
乳酸菌スターターとしてストレプトコッカス・サーモフィルス（Streptococcus thermophilus）、ラクトバチルス・デルブロッキー・サブスピーシーズ・ラクチス（Lactobacillus delbrueckii subsp. lactis）およびラクトバチルス・デルブロッキー・サブスピーシーズ・ブルガリクス（Lactobacillus delbrueckii subsp. bulgaricus）を含む市販の乳酸菌スターター（ＦＤ−ＤＶＳ ＹＣ−１８０−Ｙｏ−Ｆｌｅｘ（登録商標）：ＣＨＲ−ＨＡＮＳＥＮ社）を使用し、これを脱脂粉乳１０ｇ、水７０ｇおよびサトウキビ搾汁液（１００ｍｌあたりＧＡＢＡ含有量５.８ｍｇ、グルタミン酸含有量１.２ｍｇ、Ｂｒｉｘ２１）３０ｍｌからなる培地に８.５ｍｇ接種して３０℃で６０時間の乳酸発酵を行い、サトウキビ乳酸発酵飲料を得た。このサトウキビ乳酸発酵飲料中のＧＡＢＡ含有量は１００ｍｌあたり６.９ｍｇであった。また、このサトウキビ乳酸発酵飲料のｐＨは４.４、風味はやや酸味が強いものであった。更に、このサトウキビ乳酸発酵飲料のポリフェノール含有量はサトウキビ搾汁液を添加していない乳酸発酵飲料よりも増加し、抗酸化能もサトウキビ搾汁液を添加していない乳酸発酵飲料よりも上昇していた。
Example 3
Manufacture of sugarcane lactic acid fermented beverage (3):
Lactobacillus delbrueckii subsp. Bul, including Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Lactis and Lactobacillus delbrueckii subsp. Gar as a lactic acid bacteria starter A commercially available lactic acid bacteria starter (FD-DVS YC-180-Yo-Flex (registered trademark): CHR-HANSEN) was used, and this was mixed with skim milk powder 10 g, water 70 g and sugarcane juice (GABA content 5.8 mg per 100 ml). 8.5 mg in a medium consisting of 30 ml of glutamic acid content 1.2 mg, Brix 21) was inoculated and subjected to lactic acid fermentation for 60 hours at 30 ° C. to obtain a sugarcane lactic acid fermented beverage. The GABA content in this sugarcane lactic acid fermented beverage was 6.9 mg per 100 ml. The sugar cane lactic acid fermented beverage had a pH of 4.4 and a slightly strong sour taste. Furthermore, the polyphenol content of the sugar cane lactic acid fermented beverage was higher than that of the lactic acid fermented beverage to which no sugar cane juice was added, and the antioxidant capacity was also higher than that of the lactic acid fermented beverage to which no sugar cane juice was added.
実 施 例 ４
サトウキビ乳酸発酵飲料の製造（４）：
実施例３と同様の乳酸菌スターターを、山羊乳７０ｍｌおよび実施例３と同様のサトウキビ搾汁液３０ｍｌからなる培地に８.５ｍｇ接種して３０℃で６０時間の乳酸発酵を行い、サトウキビ乳酸発酵飲料を得た。このサトウキビ乳酸発酵飲料中の、ＧＡＢＡ含有量は１００ｍｌあたり６.２ｍｇであった。また、このサトウキビ乳酸発酵飲料のｐＨは４.１、風味はやや酸味が強いが良好な香りを有するものであった。更に、このサトウキビ乳酸発酵飲料のポリフェノール含有量はサトウキビ搾汁液を添加していない乳酸発酵飲料よりも増加し、抗酸化能もサトウキビ搾汁液を添加していない乳酸発酵飲料よりも上昇していた。
Example 4
Production of sugarcane lactic acid fermented beverage (4):
The same lactic acid bacteria starter as in Example 3 was inoculated into 8.5 mg of a medium consisting of 70 ml of goat milk and 30 ml of sugarcane juice as in Example 3, and lactic acid fermentation was performed at 30 ° C. for 60 hours. Obtained. In this sugar cane lactic acid fermented beverage, the GABA content was 6.2 mg per 100 ml. Further, the sugar cane lactic acid fermented beverage had a pH of 4.1 and a slightly strong sour taste but a good scent. Furthermore, the polyphenol content of the sugar cane lactic acid fermented beverage was higher than that of the lactic acid fermented beverage to which no sugar cane juice was added, and the antioxidant capacity was also higher than that of the lactic acid fermented beverage to which no sugar cane juice was added.
実 施 例 ５
サトウキビ乳酸発酵飲料の製造（５）：
実施例３と同様の乳酸菌スターターを、脱脂粉乳を３ｇ、山羊乳７０ｍｌおよび実施例３と同様のサトウキビ搾汁液３０ｍｌからなる培地に８.５ｍｇ接種して３０℃で６０時間の乳酸発酵を行い、サトウキビ乳酸発酵飲料を得た。このサトウキビ乳酸発酵飲料中の、ＧＡＢＡ含有量は８.２ｍｇであった。また、このサトウキビ乳酸発酵飲料のｐＨは４.２、風味はやや酸味が強いが良好な香りを有するものであった。更に、このサトウキビ乳酸発酵飲料のポリフェノール含有量はサトウキビ搾汁液を添加していない乳酸発酵飲料よりも増加し、抗酸化能もサトウキビ搾汁液を添加していない乳酸発酵飲料よりも上昇していた。
Example 5
Manufacture of sugarcane lactic acid fermented beverage (5):
Lactic acid bacteria starter similar to Example 3, 8.5g inoculated into 3g of skim milk powder, 70ml of goat milk and 30ml of sugarcane juice similar to Example 3, and lactic acid fermentation at 30 ° C for 60 hours, A sugarcane lactic acid fermented beverage was obtained. The GABA content in this sugarcane lactic acid fermented beverage was 8.2 mg. Further, the sugar cane lactic acid fermented beverage had a pH of 4.2 and a slightly strong sour taste but a good scent. Furthermore, the polyphenol content of the sugar cane lactic acid fermented beverage was higher than that of the lactic acid fermented beverage to which no sugar cane juice was added, and the antioxidant capacity was also higher than that of the lactic acid fermented beverage to which no sugar cane juice was added.
実 施 例 ６
サトウキビ乳酸発酵飲料の製造（６）：
実施例３と同様の乳酸菌スターターを、山羊乳７０ｍｌおよび実施例３と同様のサトウキビ搾汁液３０ｍｌからなる培地に８.５ｍｇ接種して３５〜４３℃で２４時間の乳酸発酵を行い、サトウキビ乳酸発酵飲料を得た。このサトウキビ乳酸発酵飲料中のＧＡＢＡ含有量は１２ｍｇであった。また、このサトウキビ乳酸発酵飲料のｐＨは３.６、風味はやや酸味が強いものの甘味とバランスし、良好な香りを有するものであった。更に、このサトウキビ乳酸発酵飲料のポリフェノール含有量はサトウキビ搾汁液を添加していない乳酸発酵飲料よりも増加し、抗酸化能もサトウキビ搾汁液を添加していない乳酸発酵飲料よりも上昇していた。
Example 6
Production of sugarcane lactic acid fermented beverage (6):
8.5 mg of a lactic acid bacteria starter similar to that in Example 3 was inoculated into a medium comprising 70 ml of goat milk and 30 ml of sugarcane juice similar to that in Example 3, and lactic acid fermentation was carried out at 35 to 43 ° C. for 24 hours. I got a drink. The GABA content in this sugarcane lactic acid fermented beverage was 12 mg. The sugar cane lactic acid fermented beverage had a pH of 3.6, a slightly strong sour taste, and a good aroma. Furthermore, the polyphenol content of the sugar cane lactic acid fermented beverage was higher than that of the lactic acid fermented beverage to which no sugar cane juice was added, and the antioxidant capacity was also higher than that of the lactic acid fermented beverage to which no sugar cane juice was added.
本発明のサトウキビ乳酸発酵飲料はポリフェノール、γ−アミノ酪酸等の成分および優れた抗酸化能を有するので、これらの効果を期待した健康飲料等に好適に利用することができる。
以 上
Since the sugarcane lactic acid fermented beverage of the present invention has components such as polyphenol and γ-aminobutyric acid and excellent antioxidant ability, it can be suitably used for health drinks and the like that expect these effects.
more than
Claims (6)
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| JP2017127243A (en) * | 2016-01-20 | 2017-07-27 | 大阪瓦斯株式会社 | Fermentation beverage and method for producing the same |
| JP2017148391A (en) * | 2016-02-26 | 2017-08-31 | 榮治 上原 | Vapor bath method, vapor bath facility, and vapor bath device |
| CN111690573A (en) * | 2020-07-22 | 2020-09-22 | 湛江银恒生物科技有限公司 | Lactobacillus hilgardii for animal intestinal probiotics, ferment prepared by fermentation of lactobacillus hilgardii and application of ferment |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011193858A (en) * | 2010-03-24 | 2011-10-06 | National Agriculture & Food Research Organization | γ-AMINOBUTYRIC ACID-CONTAINING FEED, METHOD FOR PRODUCING THE SAME, AND METHOD FOR RAISING LIVESTOCK USING THE FEED |
| JP2017127243A (en) * | 2016-01-20 | 2017-07-27 | 大阪瓦斯株式会社 | Fermentation beverage and method for producing the same |
| JP2017148391A (en) * | 2016-02-26 | 2017-08-31 | 榮治 上原 | Vapor bath method, vapor bath facility, and vapor bath device |
| CN111690573A (en) * | 2020-07-22 | 2020-09-22 | 湛江银恒生物科技有限公司 | Lactobacillus hilgardii for animal intestinal probiotics, ferment prepared by fermentation of lactobacillus hilgardii and application of ferment |
| CN111690573B (en) * | 2020-07-22 | 2023-05-09 | 湛江银恒生物科技有限公司 | Lactobacillus johnsonii for animal intestinal probiotics and ferment prepared by fermenting same and application thereof |
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