JP2007248426A - Microchip, and inspection system using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microchip capable of reducing a measured noise to provide a highly precise inspection result, and an inspection system using the small-sized inexpensive microchip. <P>SOLUTION: This microchip having a base material formed with at least the first flow passage with the first fluid flowing therethrough, the second flow passage with the second fluid flowing therethrough, and a confluence flow passage joined with the first flow passage and the second flow passage, and a lid material for covering the flow passages in the base material, has wavelength selectivity of bringing a spectral transmittance of a detected part of the lid material irradiated with light for detecting a mixing or reaction state of a fluid flowing through the confluence flow passage, into a specified relation with respect to at least one spectrum, out of an emission spectrum of the irradiated light and an absorption spectrum characterizing the mixing or reaction state in the fluid. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a microchip and an inspection system using the microchip.

  In recent years, by making full use of micromachine technology and ultrafine processing technology, devices and means (for example, pumps, valves, flow paths, sensors, etc.) for performing chemical analysis, chemical synthesis, etc. are miniaturized and integrated on one chip. System has been developed. This is also referred to as μ-TAS (Micro total Analysis System), and a reagent solution and a sample solution (for example, urine, saliva, blood, DNA of a subject to be tested are extracted with DNA) on a member called a microchip. This is a method for investigating the characteristics of a specimen by merging solutions and the like and detecting the reaction.

  Microchip performs injection molding, photolithographic process (a method of creating a groove by etching a pattern image with chemicals) on a substrate made of a resin material or a glass material, or groove processing using laser light. Various flow patterns have been proposed which are provided with a fine flow path through which a reagent solution can flow and a liquid reservoir for storing the reagent.

  Furthermore, this μ-TAS is expected to be applied in the medical examination, diagnostic field, environmental measurement field, and agricultural production field. In reality, as is seen in genetic testing, when complicated processes, skilled techniques, and equipment operations are required, automation, acceleration, and simplification of the micro total analysis system enable cost and necessity. The analysis can be performed not only on the test amount and the time required, but also on any time and place, and the benefits are great.

  In various types of analysis and inspection, the accuracy and reliability of reaction detection in these microchips are regarded as important.

  In particular, as a means for inspecting reagent mixing and chemical reaction results, for example, there is a reaction detection device that uses an absorptiometer to measure a partial discoloration of a specimen due to reagent reaction with high accuracy. Proposed in Patent Document 1 is a reference where a specimen flow path and a reagent flow path merge, a reaction detection part is provided in the vicinity of the merge flow path so that the reaction between the specimen and the reagent can be detected, and the specimen can be detected. Providing a reaction detected portion has been proposed. In addition to the reaction test with the reagent, for example, the detection of noise components and the characteristics of the specimen are performed, and the detection result from the reaction detection part is detected by the reaction detection part based on the detection result from the reference detection part. The accuracy of the test can be improved by correcting the noise component or controlling the mixing with the reagent in accordance with the characteristics of the specimen.

  Patent Document 2 describes means for removing fluorescence noise light emitted from a microchip substrate when light is applied to a specimen to detect fluorescence. In other words, the plastic substrate for microchip, which is made of plastic that has been modified to prevent the penetration of excitation light received by the plastic used for the substrate and prevent the fluorescence emitted by the excitation light from reaching the detector Is described.

  Patent Document 3 discloses a homopolymer composed of 1,3-cyclohexadiene (CHD) or a CHD derivative, which exhibits excellent light transmittance with respect to a light source having a specific ultraviolet wavelength, and other copolymerizable with these. By creating a resin microchip using a processing method such as injection molding with a polymer obtained by hydrogenating a polymer obtained by copolymerization with a monomer, it has excellent light transmittance in the ultraviolet wavelength region of 230 to 400 nm. Providing a microchip for optical analysis.

In Patent Document 4, a pair of quartz glass substrates and an alkali ion-containing glass layer are sandwiched between these quartz glass substrates to function as an adhesive member between the quartz glass substrates, and light in a practical ultraviolet to visible wavelength region is used. It is described to provide a microchannel device that improves the permeability of the liquid.
JP 2003-4752 A JP 2003-130874 A JP 2000-39420 A JP 2001-349826 A

However, in order to perform a high-precision inspection, the influence of noise at the time of light detection cannot be ignored. However, in the above-mentioned patent document, a proposal to make the base material a highly transmissive member and noise light emitted as fluorescence are removed. However, various measures must be taken when measuring a minute density difference or the like as a test result.

  For example, it is necessary to remove the weak background light that becomes noise, and it is necessary to increase the wavelength matching between the light source and the measurement target (for example, a color reaction result) in order to increase the detection signal. Therefore, in the past, measurements were often made with a great deal of ingenuity such as strong light shielding, a chopper between the light source and light reception, and advanced signal processing to extract detection signals buried in noise. Costs are often constrained.

  In addition, when there are multiple types of diagnostic chips used in the diagnostic system and the types and reactions of the reagents differ, it is necessary to select a wavelength that is sensitive to light detection (low noise and high sensitivity), but each has a suitable spectral sensitivity. However, a large number of optical filters and the like are prepared on the apparatus side so as to match the above, and a switching mechanism is provided. However, there are disadvantages such as an increase in apparatus size and an increase in manufacturing cost.

  Furthermore, for example, an optical filter such as an interference filter generally tends to cause characteristic changes due to moisture absorption (changes in spectral characteristics), and in order to maintain the characteristics for a long period of time, it is necessary to take sufficient measures against moisture in the apparatus. . Also, if dust, dirt, or cloudiness adheres to the filter, an error occurs, so attention should be paid to maintenance.

  An object of the present invention is to provide a microchip capable of reducing measurement noise and obtaining a highly accurate inspection result, and an inspection system using a small and low-cost microchip.

The above problem can be solved by the following configuration.
1. A first flow path through which the first fluid flows, a second flow path through which the second fluid flows, and a merging flow path where the first flow path and the second flow path merge are formed at least. A base material,
A lid covering the channel of the substrate;
In a microchip having
Spectral transmittance of the detected portion of the lid member irradiated with light to detect the mixing or reaction state of the fluid flowing through the confluence channel is the emission spectrum of the irradiation light and the mixing state or reaction state of the fluid. A microchip having wavelength selectivity that has a specific relationship to at least one of the absorption spectra to be characterized.
2. The half-value width (λ1 ± Δλ1) of the spectral transmittance is (λ2 ± Δλ2) ≦ (λ1 ± Δλ1) ≦ 3 × (λ2 ± Δλ2) with respect to the half-value width (λ2 ± Δλ2) of the emission spectrum.
2. The microchip according to 1, wherein
Note that the equation represented by (λ ± Δλ) indicates a wavelength range surrounded by the half-value width of the peak wavelength λ.
3. The half-value width (λ1 ± Δλ1) of the spectral transmittance is (λ3 ± Δλ3) ≦ (λ1 ± Δλ1) ≦ 3 × (λ3 ± Δλ3) with respect to the half-value width (λ3 ± Δλ3) of the absorption spectrum.
2. The microchip according to 1, wherein
Note that the equation represented by (λ ± Δλ) indicates a wavelength range surrounded by the half-value width of the peak wavelength λ.
4). 4. The microchip according to 2 or 3, wherein the half width (λ1 ± Δλ1) of the spectral transmittance exists in a visible light region.
5). 5. The microchip according to any one of 2 to 4, wherein an absolute value of the spectral transmittance within a range of the half-value width (λ1 ± Δλ1) of the spectral transmittance is 50% or more.
6). The spectral transmittance of a region outside the half width (λ2 ± Δλ2) of the emission spectrum or four times the half width (λ3 ± Δλ3) of the absorption spectrum is the maximum spectral transmission of the inner region. The microchip according to any one of 2 to 5, wherein the microchip is 1/10 or less of the rate.
7). The detected portion of the lid member is constituted by the material having the wavelength selectivity, the coating of the material having the wavelength selectivity, or the inclusion of the material having the wavelength selectivity. A microchip according to claim 1.
8). The microchip according to any one of 1 to 7, wherein a surface opposite to the surface irradiated with the irradiation light has high reflectivity.
A microchip according to 9.1 to 8,
A microchip housing portion capable of housing the microchip;
A light source for irradiating light to the detected part of the microchip housed in the microchip housing part;
A light receiving unit that receives light from the detected portion of the microchip housed in the microchip housing unit;
An inspection system using a microchip, comprising:

According to the present invention, since the wavelength selectivity function is provided on the microchip side instead of the inspection apparatus side, a small and low-cost apparatus can be realized, and measurement noise can be reduced and high-precision inspection results can be obtained. Can do.

In the present embodiment, as an example, a case where a sample and a reagent are reacted on a microchip, such as a DNA chip that detects a reaction of a specific DNA, is not limited to this, but at least two types of fluids are microchip. It can be applied when mixing above.
<Device configuration>
FIG. 1 is an external view of an inspection apparatus 80 using the microchip according to the present embodiment. The inspection device 80 is a device that automatically reacts a specimen and a reagent previously injected into the microchip 1 and automatically outputs a reaction result.

  The casing 82 of the inspection device 80 is provided with an insertion port 83 for inserting the microchip 1 into the device, a display unit 84, a memory card slot 85, a print output port 86, an operation panel 87, and an external input / output terminal 88. It has been.

  The person in charge of inspection inserts the microchip 1 in the direction of the arrow in FIG. 1 and operates the operation panel 87 to start the inspection. Inside the inspection device 80, the reaction in the microchip 1 is automatically inspected, and when the inspection is completed, the result is displayed on the display unit 84. The inspection result can be output from the print output port 86 or stored in a memory card inserted into the memory card slot 85 by operating the operation panel 87. Further, data can be stored in the personal computer or the like from the external input / output terminal 88 using, for example, a LAN cable. After completion of the inspection, the inspection person takes out the microchip 1 from the insertion port 83.

  FIG. 2 is a configuration diagram of an inspection apparatus 80 using the microchip according to the present embodiment. FIG. 2 shows a state where the microchip is inserted from the insertion port 83 shown in FIG. 1 and the setting is completed.

  The inspection apparatus 80 includes a driving liquid tank 10 that stores a driving liquid 11 for feeding a sample and a reagent previously injected into the microchip 1, a pump 5 for supplying the driving liquid 11 to the microchip 1, and a pump 5. And a microchip 1 are connected so that the driving liquid 11 does not leak, a temperature control unit 3 that controls the temperature of a necessary part of the microchip 1, and a chip that adheres to the packing 6 so that the microchip 1 is not displaced. The pressing plate 2, the pressing plate drive unit 32 for raising and lowering the chip pressing plate 2, the regulating member 31 for accurately positioning the microchip 1 with respect to the pump 5, the reaction state between the sample and the reagent in the microchip 1, etc. A light detection unit 4 for detection is provided.

  The chip pressing plate 2 is retracted upward from the position shown in FIG. 2 in the initial state. Thereby, the microchip 1 can be inserted / removed in the direction of the arrow X, and the person inspecting inserts the microchip 1 from the insertion port 83 (see FIG. 1) until it comes into contact with the regulating member 31. Thereafter, the chip pressing plate 2 is lowered by the pressing plate driving unit 32 and comes into contact with the microchip 1, and the lower surface of the microchip 1 is in close contact with the temperature adjustment unit 3 and the packing 6.

  The temperature control unit 3 controls the temperature of the necessary part of the microchip 1 and cools the part containing the reagent so that the reagent is not denatured or heats the part where the sample and the reagent react. To promote the reaction.

  The light detection unit 4 includes a light emitting unit 4a and a light receiving unit 4b. The light detecting unit 4 irradiates the microchip 1 with light from the light emitting unit 4a, and detects light transmitted through the microchip 1 with the light receiving unit 4b. The light receiving portion 4b is integrally provided inside the chip pressing plate 2. A plurality of light emitting sections 4a and light receiving sections 4b are provided so as to face each of the detected sections 111a to 111d of the microchip 1 described later.

  The pump 5 includes a pump chamber 52, a piezoelectric element 51 that changes the volume of the pump chamber 52, a first throttle channel 53 that is located on the microchip 1 side of the pump chamber 52, and a first that is located on the drive fluid tank 10 side of the pump chamber. It is composed of two throttle channels 54 and the like. The first throttle channel 53 and the second throttle channel 54 are narrow and narrow channels, and the first throttle channel 53 is longer than the second throttle channel 54.

  In the case where the driving liquid 11 is fed in the forward direction (direction toward the microchip 1), first, the piezoelectric element 51 is driven so as to rapidly reduce the volume of the pump chamber 52. Then, a turbulent flow is generated in the second throttle channel 54 that is a short throttle channel, and the channel resistance in the second throttle channel 54 is relatively larger than that of the first throttle channel 53 that is a throttle channel. growing. As a result, the driving liquid 11 in the pump chamber 52 is predominantly pushed toward the first throttle channel 53 and fed. Next, the piezoelectric element 51 is driven so that the volume of the pump chamber 52 is gradually increased. Then, the driving liquid 11 flows from the first throttle channel 53 and the second throttle channel 54 as the volume in the pump chamber 52 increases. At this time, since the length of the second throttle channel 54 is shorter than that of the first throttle channel 53, the channel resistance of the second throttle channel 54 is smaller than that of the first throttle channel 53. Thus, the driving liquid 11 flows predominantly from the second throttle channel 54 into the pump chamber 52. When the piezoelectric element 51 repeats the above operation, the driving liquid 11 is fed in the forward direction.

On the other hand, when the driving liquid 11 is fed in the reverse direction (direction toward the driving liquid tank 10), first, the piezoelectric element 51 is driven so that the volume of the pump chamber 52 is gradually reduced. Then, since the length of the second throttle channel 54 is shorter than that of the first throttle channel 53, the channel resistance of the second throttle channel 54 is smaller than that of the first throttle channel 53. . As a result, the driving liquid 11 in the pump chamber 52 is predominantly pushed out toward the second throttle channel 54 and fed. Next, the piezoelectric element 51 is driven so that the volume of the pump chamber 52 is rapidly increased. Then, the driving liquid 11 flows from the first throttle channel 53 and the second throttle channel 54 as the volume in the pump chamber 52 increases. At this time, turbulent flow is generated in the second throttle channel 54, which is a short throttle channel, and the channel resistance in the second throttle channel 54 is relatively larger than that of the first throttle channel 53, which is a throttle channel. Become bigger. As a result, the driving liquid 11 flows into the pump chamber 52 predominantly from the first throttle channel 53. When the piezoelectric element 51 repeats the above operation, the driving liquid 11 is fed in the reverse direction.
<Configuration of microchip>
FIG. 3 is a configuration diagram of the microchip 1 according to the present embodiment. An example configuration is shown, and the present invention is not limited to this.

  In FIG. 3A, an arrow indicates an insertion direction in which the microchip 1 is inserted into the inspection apparatus 80, and FIG. 3A illustrates a surface serving as a lower surface of the microchip 1 when inserted. FIG. 3B is a side view of the microchip 1.

  As shown in FIG. 3B, the microchip 1 includes a groove forming substrate 108 and a covering substrate 109 that covers the groove forming substrate 108.

  The microchip 1 according to the present embodiment is provided with a fine channel and a channel element for mixing and reacting a specimen and a reagent on the microchip 1. An example of processing performed in the microchip 1 by these fine flow paths and flow path elements will be described with reference to FIG. FIG. 3C schematically shows the flow path element and its joined state with the coated substrate 109 removed in FIG.

  The fine flow path includes a sample storage unit 121 that stores a sample liquid, a reagent storage unit 120 that stores a reagent, a positive control storage unit 122 that stores a positive control reagent, and a negative control storage that stores a negative control reagent. A portion 123 and the like are provided. Reagents, positive controls, and negative controls are stored in advance in the storage units. The positive control reacts with the reagent and shows positive, and the negative control reacts with the reagent and shows negative, and is used to confirm whether or not an accurate test has been performed.

  FIG. 3 (c) is shown schematically for the sake of simplicity. Actually, a plurality of reagents, diluting solutions, etc. are accommodated in the chip, and the reagent is mixed in the chip. Also good.

  The sample injection unit 113 is an injection unit for injecting the sample into the microchip 1, and the driving liquid injection units 110 a to 110 d are injection units for injecting the driving liquid 11 into the microchip 1.

  First, prior to performing a test using the microchip 1, the person in charge of the test injects a sample from the sample injection unit 113 using a syringe or the like. As shown in FIG. 3C, the sample injected from the sample injection unit 113 is stored in the sample storage unit 121 through the communicating fine channel.

  Next, the microchip 1 into which the sample is injected is inserted into the insertion port 83 of the inspection apparatus 80 shown in FIG. 1 by the inspection person, and set as shown in FIG.

  Next, the pump 5 shown in FIG. 2 is driven in the forward direction according to a predetermined procedure instructed from a control unit (not shown), and the driving liquid 11 is injected from the driving liquid injection units 110a to 110d. The driving liquid 11 injected from the driving liquid injection unit 110 a pushes out the sample stored in the sample storage unit 121 through the communicating fine flow path, and sends the sample to the reaction unit 124. The driving liquid 11 injected from the driving liquid injection unit 110 b pushes out the positive control reagent stored in the positive control storage unit 122 through the communicating fine flow path, and sends the positive control to the reaction unit 125. The driving liquid 11 injected from the driving liquid injection unit 110 c pushes out the negative control reagent stored in the negative control storage unit 123 through the communicating fine channel, and sends negative control to the reaction unit 126. The driving liquid 11 injected from the driving liquid injection section 110d pushes out the reagent stored in the reagent storage section 120 through the communicating fine flow path, and sends the reagent into the reaction sections 124 to 126 described above.

  In this way, the sample and the reagent merge in the reaction unit 124, the positive control and the reagent merge in the reaction unit 125, and the negative control and the reagent merge in the reaction unit 126.

Thereafter, a part of the mixed liquid of the specimen and the reagent merged in the reaction unit 124 is sent to the detected unit 111a. A part of the mixed solution of the specimen and the reagent merged in the reaction unit 124 and a part of the mixed solution of the positive control and the reagent merged in the reaction unit 125 are sent to the detection unit 111b. A part of the mixed liquid of the positive control and the reagent merged in the reaction unit 125 is sent to the detection unit 111c. The liquid mixture of the negative control and the reagent merged in the reaction unit 126 is sent to the detected unit 111d.
<Detected part>
The spectral transmittance of the detected part 111 in the microchip 1 of the present invention will be described. In the detected part 111 of the microchip 1, the specimen and the reagent stored in the microchip 1 react to cause coloration, fluorescence by excitation light, turbidity, and the like. In this embodiment, the reaction result of the reagent is detected optically. The groove forming substrate 108 and the covering substrate 109 of the detected part 111 of the microchip 1 for measuring the reaction result of the reagent are made of a light-transmitting material, and the reaction result of the reagent and the sample is detected by the microchip 1. The light transmitted through or reflected by the unit 111 can be analyzed by photometric or colorimetric measurement.

  Here, FIG. 4A shows a transmission type example in which the light from the light emitting section 4a passes through the detected section 111 and is detected by measuring the amount of light at the light receiving section 4b. The coated substrate 109 is made of a light-transmitting resin material such as polypropylene, and the surface of the coated substrate 109 has a spectral transmittance, a reagent reaction when the emission spectrum of the light emitting part 4a and a target object are present. A material having wavelength selectivity having a specific relationship with at least one of the absorption spectra is applied. The groove forming substrate 108 is made of a resin material having optical transparency such as polypropylene. The light emitted from the light emitting unit 4a of the light detecting unit 4 passes through the reaction solution present in the detected unit 111 of the microchip and is received by the light receiving unit 4b. At this time, when the target sample target exists, the probe targeted by, for example, gold colloid is adsorbed and colored. This color reaction is measured and inspected by a transmitted light amount difference (that is, an optical density difference) before and after the reaction.

  FIG. 4B shows an example of a reflection type in which the light received from the light source is reflected by the detected portion 111 and is detected by measuring the amount of light at the light receiving portion. In this case, when the reflected light is received, it passes through the coated substrate 109 twice, and the amount of reflected light is reduced. Therefore, as shown in FIG. By processing the reflectance, for example, by performing aluminum vapor deposition or white coating, the amount of reflected light can be increased and the S / N can be improved.

Next, an example of an emission spectrum when the light emitting unit 4a is a green LED is shown in FIG. 5A, and the spectral transmittance of the coated substrate 109 is shown in FIG. 5B. If the half width of the spectral transmittance is defined as (λ1 ± Δλ1) and the half width of the emission spectrum is defined as (λ2 ± Δλ2),
(Λ1 ± Δλ1) ≧ (λ2 ± Δλ2)
It becomes. The half-value width (λ2 ± Δλ2) of the emission spectrum is included in the range of the half-value width (λ1 ± Δλ1) of the spectral transmittance.

  At this time, the absolute value of the spectral transmittance is set to 50% or more within the range of the half-value width of the spectral transmittance, thereby suppressing signal reduction due to light absorption of the chip material itself and improving the S / N ratio. Note that (λ1 ± Δλ1) and (λ2 ± Δλ2) indicate regions having a full width at half maximum with respect to the peak, and are not mathematically exact expressions.

Further, FIG. 6A shows an absorption spectrum of a reagent reaction using gold colloid as a reagent, and FIG. 6B shows a half-value width of spectral transmittance. If the half width of the absorption spectrum is (λ3 ± Δλ3),
(Λ1 ± Δλ1) ≧ (λ3 ± Δλ3)
It becomes. The half-value width (λ3 ± Δλ3) of the absorption spectrum is included in the range of the half-value width (λ1 ± Δλ1) of the spectral transmittance.

However, it is not necessary to make the half-value width of the spectral transmittance wider than necessary. Specifically, in the case of an emission spectrum,
(Λ2 ± Δλ2) ≦ (λ1 ± Δλ1) ≦ 3 (λ2 ± Δλ2)
For the absorption spectrum,
(Λ3 ± Δλ3) ≦ (λ1 ± Δλ1) ≦ 3 (λ3 ± Δλ3)
Thus, it is desirable that the half-value width (λ1 ± Δλ1) of the spectral transmittance is three times or less than the half-value width (λ2 ± Δλ2) of the emission spectrum and the half-value width (λ3 ± Δλ3) of the absorption spectrum.

  Further, as shown in FIG. 7, in order to remove the noise of the light component that becomes a disturbance until it can be almost ignored, the spectral transmittance is at most four times the half-value width of the emission spectrum (FIG. 7A), or In the region outside the maximum half-value width of the absorption spectrum by more than 4 times (FIG. 7B), the spectrum is cut off to be 1/10 or less of the maximum spectral transmittance of the region inside. I am letting.

  By making all these wavelength ranges measurable in the visible light range, the reaction result can be reconfirmed by visual color discrimination or the like in addition to automatic detection by the apparatus.

  In addition, the microchip has a structure of two or more pieces of the groove forming substrate 108 and the covering substrate 109, and a material having wavelength selectivity is applied to or included in the covering substrate 109 at the time of manufacturing the microchip to change the optical characteristics. Therefore, it is possible to compensate for a subtle characteristic difference, and it is possible to provide a highly accurate microchip.

  Furthermore, a groove | channel structure board | substrate may form a groove | channel on front and back both surfaces, and may provide a covering substrate in front and back. At this time, the Al deposition shown in FIG. 4C can also be provided on the back-coated substrate.

  Furthermore, it is clear that wavelength selectivity can be provided by coating or the like on the back surface of the groove-constituting substrate in FIGS. 4 (a) and 4 (b), and the light source can be irradiated from the back surface side of the figure. It is more preferable to provide wavelength selectivity to the coated substrate that is easy to keep flat.

  Further, the coated substrate 109 is processed with a material having a wavelength selectivity, processed, and a material or surface treatment with a gas barrier performance that shields moisture, oxygen, etc., so that the reagent in the chip is stored during microchip storage. Evaporation can be prevented, and deterioration and alteration of moisture, oxygen, and the like from the outside of the chip on the reagent in the chip can be prevented.

  As described above, according to the present embodiment, since the function of wavelength selectivity is provided on the microchip side instead of the inspection apparatus side, a small and low-cost apparatus can be realized, and measurement noise is reduced and accuracy is improved. High test results can be obtained.

  Furthermore, by making the half-width of the spectral transmittance of the microchip wider than the half-width of the light source spectrum and the light absorption spectrum, the loss of detection light due to the microchip base material and lid material can be reduced. Thus, the signal can be used effectively and the S / N ratio is improved.

It is an external view of the reaction detection apparatus 80 in embodiment of this invention. It is a block diagram of the test | inspection apparatus using the microchip which concerns on this embodiment. It is a block diagram of the microchip which concerns on this embodiment. 4A shows an example of a transmission type, and FIG. 4B shows an example of a reflection type. It is a figure which shows the half value width of an emission spectrum and spectral transmittance. It is a figure which shows the relationship between the absorption spectrum of a reagent reaction, and the half value width of spectral transmittance. Outside the half-width region of the spectral transmittance, the spectrum is cut off to 1/10.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Microchip 4 Light detection part 80 Test | inspection apparatus 108 Groove formation board 109 Coated board | substrate 111 Detected part 120 Reagent storage part 121 Specimen storage part 122 Positive control storage part 123 Negative control storage part 124, 125, 126 Reaction part

Claims (9)

A first flow path through which the first fluid flows, a second flow path through which the second fluid flows, and a merging flow path where the first flow path and the second flow path merge are formed at least. A base material,
A lid covering the channel of the substrate;
In a microchip having
Spectral transmittance of the detected portion of the lid member irradiated with light to detect the mixing or reaction state of the fluid flowing through the confluence channel is the emission spectrum of the irradiation light and the mixing state or reaction state of the fluid. A microchip having wavelength selectivity that has a specific relationship to at least one of the absorption spectra to be characterized. The half-value width (λ1 ± Δλ1) of the spectral transmittance is (λ2 ± Δλ2) ≦ (λ1 ± Δλ1) ≦ 3 × (λ2 ± Δλ2) with respect to the half-value width (λ2 ± Δλ2) of the emission spectrum.
The microchip according to claim 1, wherein:
Note that the equation represented by (λ ± Δλ) indicates a wavelength range surrounded by the half-value width of the peak wavelength λ. The half width of the spectral transmittance (λ1 ± Δλ1) is (λ3 ± Δλ3) ≦ (λ1 ± Δλ1) ≦ 3 × (λ3 ± Δλ3) with respect to the half width of the absorption spectrum (λ3 ± Δλ3).
The microchip according to claim 1, wherein:
Note that the equation represented by (λ ± Δλ) indicates a wavelength range surrounded by the half-value width of the peak wavelength λ. 4. The microchip according to claim 2, wherein the half-value width (λ1 ± Δλ1) of the spectral transmittance exists in a visible light region. 5. The microchip according to claim 2, wherein an absolute value of the spectral transmittance within a range of a half-value width (λ1 ± Δλ1) of the spectral transmittance is 50% or more. The spectral transmittance of a region outside the half width (λ2 ± Δλ2) of the emission spectrum or four times the half width (λ3 ± Δλ3) of the absorption spectrum is the maximum spectral transmission of the inner region. The microchip according to claim 2, wherein the microchip is 1/10 or less of the rate. The detected portion of the lid member is configured by the material having the wavelength selectivity, the application of the material having the wavelength selectivity, or the inclusion of the material having the wavelength selectivity. 7. The microchip according to 6. The microchip according to claim 1, wherein a surface opposite to the surface irradiated with the irradiation light has high reflectivity. A microchip according to claim 1 to 8,
A microchip housing portion capable of housing the microchip;
A light source for irradiating light to the detected part of the microchip housed in the microchip housing part;
A light receiving unit that receives light from the detected portion of the microchip housed in the microchip housing unit;
An inspection system using a microchip, comprising:

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