JP2007202573A - Culture apparatus - Google Patents

Culture apparatus Download PDF

Info

Publication number
JP2007202573A
JP2007202573A JP2007134855A JP2007134855A JP2007202573A JP 2007202573 A JP2007202573 A JP 2007202573A JP 2007134855 A JP2007134855 A JP 2007134855A JP 2007134855 A JP2007134855 A JP 2007134855A JP 2007202573 A JP2007202573 A JP 2007202573A
Authority
JP
Japan
Prior art keywords
culture
culture apparatus
shelves
storage
container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2007134855A
Other languages
Japanese (ja)
Other versions
JP4236687B2 (en
Inventor
Yuichi Tamaoki
裕一 玉置
Noriyuki Arai
敬之 新井
Tadahisa Saga
忠久 佐賀
Hiroki Busujima
弘樹 毒島
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanyo Electric Co Ltd
Original Assignee
Sanyo Electric Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanyo Electric Co Ltd filed Critical Sanyo Electric Co Ltd
Priority to JP2007134855A priority Critical patent/JP4236687B2/en
Publication of JP2007202573A publication Critical patent/JP2007202573A/en
Application granted granted Critical
Publication of JP4236687B2 publication Critical patent/JP4236687B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

<P>PROBLEM TO BE SOLVED: To provide a culture apparatus which receives many culture containers without shaking. <P>SOLUTION: This culture apparatus is characterized by having circularly arranged culture container-receiving shelves (12a, 12b...) and a culture container-conveying means (15) on the insides of the circularly arranged culture containers. The culture apparatus preferably comprises the circularly arranged culture container-receiving shelves (12a, 12b...), a table (13) for supporting the shelves, an air-circulating port (39) disposed in the table (13) so that air on the circular insides are circulated from the lower side to the upper side, and a circulation route (36) for returning the air sucked from the upper side of the tank to the lower portion. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

本発明は、細菌等を保管する保管庫又は培養する培養装置に関するものである。商品名で言えば、自動培養システム、血液保存庫、凍結血漿保存庫、医薬品保存庫等に関する。   The present invention relates to a storage for storing bacteria or the like, or a culture apparatus for culturing. In terms of product names, it relates to an automatic culture system, a blood storage, a frozen plasma storage, a medicine storage, and the like.

細菌等を培養する培養装置においては、たくさんの細菌等を培養するものがある。最近では、このタイプのものが要求されている。   Some culture apparatuses for culturing bacteria and the like cultivate many bacteria and the like. Recently, this type has been required.

図３を参照しつつ、このような培養装置を説明する。   Such a culture apparatus will be described with reference to FIG.

１は、培養装置である。この培養措置１は、ロボットにより外部での培養容器の搬送を行っている。この培養装置１の庫内には、培養容器５の収納棚２ａ，２ｂ，２ｃが設置されている。そして、この収納棚２ａ，２ｂ，２ｃは、円弧状に配置され、且つ、回転テーブル３の上に設けられている。４は、搬送機構である。この搬送機構４は、培養容器５を任意の位置の収納棚に出し入れする。培養容器５は、前記棚２ａ，２ｂ，２ｃに複数収納される。   Reference numeral 1 denotes a culture apparatus. In this culture measure 1, a culture vessel is transported outside by a robot. In the storage of the culture apparatus 1, storage shelves 2a, 2b, 2c for the culture container 5 are installed. The storage shelves 2 a, 2 b, 2 c are arranged in an arc shape and are provided on the turntable 3. Reference numeral 4 denotes a transport mechanism. The transport mechanism 4 takes the culture container 5 into and out of a storage shelf at an arbitrary position. A plurality of culture vessels 5 are stored in the shelves 2a, 2b, 2c.

６は、培養装置の外部の搬送機構７に対して培養容器５の受け渡しを行う搬送口である。   Reference numeral 6 denotes a transfer port for delivering the culture container 5 to the transfer mechanism 7 outside the culture apparatus.

搬送機構４が、収納棚の任意の培養容器を取り出すには、まず、回転テーブル３を回転させて、収納棚を搬送機構４の前に回転移動させる必要がある。   In order for the transport mechanism 4 to take out an arbitrary culture vessel on the storage shelf, first, the rotary table 3 needs to be rotated to rotate the storage shelf in front of the transport mechanism 4.

このとき、棚２ａ，２ｂ，２ｃの全ての収納棚が回転する。そして、ひとつの培養容器を出し入れする。   At this time, all the storage shelves of the shelves 2a, 2b, and 2c rotate. Then, one culture container is taken in and out.

このように、出し入れの度に他の全ての培養容器も回転させられるため、結果的には静置状態はほとんど無く、細胞育成の生成ストレスとなる。   In this way, every other culture vessel is rotated every time it is put in and out, so that there is almost no stationary state, resulting in generation stress for cell growth.

培養物の出し入れは、非常に頻繁に行うので、搬送動作の更なる高速化が求められている。   Since the culture is taken in and out very frequently, further speeding up of the transfer operation is required.

しかし、このような理由から回転テーブル３の回転速度をむやみに速くするわけにはいかない。
特開平０７−２４２３０６号公報 However, for this reason, the rotation speed of the turntable 3 cannot be increased unnecessarily.
Japanese Patent Application Laid-Open No. 07-242306

従来の搬送機構付きの培養装置は、回転式の培養容器収納棚を利用している。   A conventional culture apparatus with a transport mechanism uses a rotary culture container storage shelf.

ところで、培養物の多くは培養容器の壁面に付着することにより正常に増殖する。従来では、所定の培養容器を搬送するために、棚全体を回転させ搬送手段の前まで移動させる必要があるため、培養容器が頻繁に振動し、細胞が正常に容器に付着することができないことがある。   By the way, many cultures grow normally by adhering to the wall surface of the culture vessel. Conventionally, in order to transport a predetermined culture container, it is necessary to rotate the entire shelf and move it to the front of the transport means, so that the culture container vibrates frequently and the cells cannot normally adhere to the container. There is.

本発明は、配置が円弧又は環状に配置される培養容器の収納棚(12a,12b・・)と、前記円弧又は環状配置の内側に培養容器の搬送手段(15)を備えたことを特徴とする。   The present invention is characterized in that the culture vessel storage shelves (12a, 12b,...) Arranged in an arc or ring arrangement, and culture vessel transport means (15) inside the arc or ring arrangement. To do.

また、本発明は、配置が円弧状、または、環状に配置される培養容器の収納棚(12a,12b・・)と、それを支持するテーブル(13)と、前記の円弧状、環状の内側の空気が下から上へ循環するように前記テーブル(13)に設けられた空気の流通口(39)と、庫内上方から吸い込んだ空気を庫内下方に戻すための循環路(36)とを備えることを特徴とする。   Further, the present invention provides a culture vessel storage shelf (12a, 12b,...) Arranged in an arc shape or in an annular shape, a table (13) for supporting the shelf, and the arc shape and annular inner side. An air circulation port (39) provided in the table (13) so that the air of the air circulates from the bottom to the top, and a circulation path (36) for returning the air sucked from the upper side of the chamber to the lower side of the chamber It is characterized by providing.

また、本発明は、前記円弧状又は環状配置の延長である位置に前記培養容器の搬送口を配置したことを特徴とする。   In addition, the present invention is characterized in that a transport port for the culture vessel is arranged at a position that is an extension of the arcuate or annular arrangement.

また、本発明は、庫内を密閉するための扉を備え、それよりも小さい搬送用扉を前記扉と同じ側に有することを特徴とする。   Moreover, this invention is provided with the door for sealing the inside of a store | warehouse | chamber, and has the door for conveyance smaller than it on the same side as the said door, It is characterized by the above-mentioned.

以上説明したように、本発明によれば、培養物の搬送のために、培養物を保持する棚を移動することがないので振動による細胞へのストレスを最小限にできる。   As described above, according to the present invention, since the culture shelf is not moved for transporting the culture, the stress on the cells due to vibration can be minimized.

本発明の第1の実施形態を図１、図２を参照しつつ説明する。   A first embodiment of the present invention will be described with reference to FIGS.

図１は培養装置の一部を透過した図である。   FIG. 1 is a view through a part of the culture apparatus.

培養装置１１の内部には、収納棚１２ａ，１２ｂ，１２ｃ・・・が、円弧状に配置される。この収納棚１２ａ，１２ｂ，１２ｃ・・・は、テーブル１３の上に設置されている。   Inside the culture apparatus 11, storage shelves 12a, 12b, 12c... Are arranged in an arc shape. The storage shelves 12 a, 12 b, 12 c... Are installed on the table 13.

テーブル１３は、培養中及び培養物の移動中に回転することはない。   The table 13 does not rotate during the culture and the movement of the culture.

従って、棚１２ａ，１２ｂ，１２ｃ・・・も移動せず、振動も発生しない。   Therefore, the shelves 12a, 12b, 12c... Do not move and no vibration is generated.

支持部１４は、昇降軸１７を支持する。この昇降軸１７を中心に可動するのが搬送機構１５である。   The support part 14 supports the lifting shaft 17. The transport mechanism 15 is movable around the lifting shaft 17.

培養容器１８は、搬送機構１５で所定の収納棚の所定の位置に収納及び搬出される。   The culture container 18 is stored and unloaded at a predetermined position on a predetermined storage shelf by the transport mechanism 15.

図示省略したが、扉１６には外部の搬送機構と培養容器の受け渡しをする搬送口がある。   Although not shown in the drawings, the door 16 has an external transfer mechanism and a transfer port for transferring the culture vessel.

図２は上面からの図である。   FIG. 2 is a top view.

培養容器１８を保持する収納棚１２ａ，１２ｂ，１２ｃ・・・は、全体として円弧３１、３２を構成するように配置されている。   The storage shelves 12a, 12b, 12c,... Holding the culture vessel 18 are arranged so as to constitute arcs 31, 32 as a whole.

搬出機構１５は、昇降軸１７を中心に回転可能で、培養容器１８を搬出するために伸縮可能なアームとなっており、そのアームを伸ばして収納棚中の培養容器をつかむと、アームを縮めた状態３３となり、昇降軸１７を中心に回転し、さらに搬送口の高さまで昇降し状態３４となる。   The unloading mechanism 15 is rotatable about an elevating shaft 17 and is an extendable arm for unloading the culture vessel 18. When the arm is extended and the culture vessel in the storage shelf is grasped, the arm is contracted. Rotate about the elevating shaft 17 and then move up and down to the height of the transfer port to enter the state 34.

ここでアームを伸ばし、培養容器を外部の搬送機器と受け渡すことが可能となる。   Here, the arm can be extended, and the culture container can be transferred to an external transfer device.

この実施形態によれば、搬送機構が搬送中に、他の培養容器の上を通らないので、万一の落下等による被害が少ない。また、動作が少ないので、搬送の高速化が行える。   According to this embodiment, since the transport mechanism does not pass over other culture vessels during transport, there is little damage due to a drop or the like. Moreover, since there are few operations, the conveyance speed can be increased.

また、円弧３１内，円弧３２外の中で動作するので、余分な動作空間を必要としない。そのため、小型の培養装置に多くの培養物を収納することができる。   Further, since it operates in the arc 31 and outside the arc 32, no extra operation space is required. Therefore, many cultures can be stored in a small culture device.

また、搬送口は、円弧３１，３２の円弧の延長線上に配置されるので、搬送口に培養容器を移動させるための特別な手段が必要ない。   In addition, since the transfer port is disposed on an extension line of the arcs 31 and 32, no special means for moving the culture vessel to the transfer port is necessary.

前面が開いた円弧状に複数の収納棚が配置されているので、培養容器や搬送機構の状況が見やすく、またメンテナンスも容易である。   Since a plurality of storage shelves are arranged in a circular arc shape with an open front surface, it is easy to see the state of the culture vessel and the transport mechanism, and maintenance is also easy.

庫内３８の内、培養容器のある培養空間を貫通穴３９を通って下から上へ上昇し、循環路３６に回収され下へ降りる。これにより庫内の温度やガス濃度を均一に維持することが可能である。   Of the chamber 38, the culture space in which the culture vessel is located rises from the bottom through the through hole 39, is collected in the circulation path 36, and descends downward. This makes it possible to maintain the temperature and gas concentration in the cabinet uniformly.

以上説明したように、本発明は、培養物の搬送のために、培養容器を保持する収納棚を移動することがないので振動による細胞へのストレスを最小限にできる。培養物を円弧状に配置し搬送機構は培養物が円弧状に並べられている。そのため、搬送時間の短縮及び振動の低減が達成できる。また、回転テーブルがないので搬送機構の高速化が容易である。   As described above, the present invention does not move the storage shelf that holds the culture vessel for transporting the culture, so that the stress on the cells due to vibration can be minimized. The culture is arranged in an arc shape, and the culture is arranged in an arc shape in the transport mechanism. Therefore, shortening of conveyance time and reduction of vibration can be achieved. Further, since there is no rotary table, it is easy to increase the speed of the transport mechanism.

更に、搬送機構は、前記円弧の内側にあるため、搬送機構の設置、及び動作のため必要とする余分な空間を少なくすることができるので、小型の培養器でも多くの培養物が収納できる。   Furthermore, since the transport mechanism is inside the arc, the extra space required for the installation and operation of the transport mechanism can be reduced, so that a large number of cultures can be stored even in a small incubator.

収納物が密に収納されると庫内の温度分布が悪くなりがちだが、本発明では収納棚で環状に囲まれた内側にも空気の流路を構成するので、温度分布を悪化させない。   If the stored items are stored densely, the temperature distribution in the storage tends to be deteriorated. However, in the present invention, the air flow path is also formed inside the storage shelf, and thus the temperature distribution is not deteriorated.

本発明の第1実施形態である培養装置の一部を透過した斜視図である。FIG. 2 is a perspective view through a part of the culture apparatus according to the first embodiment of the present invention. この第1実施形態の上面からの断面図である。FIG. 2 is a cross-sectional view from the top surface of the first embodiment. 従来の培養装置を示す斜視図である。It is a perspective view which shows the conventional culture apparatus.

符号の説明Explanation of symbols

(12a,12b・・)収納棚
(15)搬送手段
(13)テーブル
(39)流通口
(36)循環路 (12a, 12b ...) Storage shelf
(15) Conveying means
(13) Table
(39) Distribution outlet
(36) Circuit

Claims (3)

配置が円弧又は環状に配置される培養容器の収納棚(12a,12b・・)と、
前記円弧又は環状配置の内側に培養容器の搬送手段(15)を備えたことを特徴とする培養装置。 Storage racks (12a, 12b,...) For culture vessels arranged in an arc or ring, and
A culture apparatus comprising a culture container transport means (15) inside the circular arc or annular arrangement. 前記円弧状又は環状配置の延長である位置に前記培養容器の搬送口を配置したことを特徴とする請求項１に記載の培養装置。 The culture apparatus according to claim 1, wherein a transport port of the culture vessel is arranged at a position that is an extension of the arcuate or annular arrangement. 庫内を密閉するための扉を備え、それよりも小さい搬送用扉を前記扉と同じ側に有することを特徴とする請求項１または請求項２に記載の培養装置。 The culture apparatus according to claim 1 or 2, further comprising a door for sealing the inside of the refrigerator, and having a smaller transfer door on the same side as the door.
JP2007134855A 2007-05-21 2007-05-21 Incubator Expired - Fee Related JP4236687B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007134855A JP4236687B2 (en) 2007-05-21 2007-05-21 Incubator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2007134855A JP4236687B2 (en) 2007-05-21 2007-05-21 Incubator

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2002047235A Division JP4004310B2 (en) 2002-02-22 2002-02-22 Incubator

Publications (2)

Publication Number Publication Date
JP2007202573A true JP2007202573A (en) 2007-08-16
JP4236687B2 JP4236687B2 (en) 2009-03-11

Family

ID=38482589

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007134855A Expired - Fee Related JP4236687B2 (en) 2007-05-21 2007-05-21 Incubator

Country Status (1)

Country Link
JP (1) JP4236687B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010063460A (en) * 2009-10-16 2010-03-25 Korea Inst Of Geoscience & Mineral Resources (Kigam) Apparatus for storing sample
WO2015046237A1 (en) * 2013-09-26 2015-04-02 東京エレクトロン株式会社 Cell culturing apparatus and cell culturing system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010063460A (en) * 2009-10-16 2010-03-25 Korea Inst Of Geoscience & Mineral Resources (Kigam) Apparatus for storing sample
JP4560130B2 (en) * 2009-10-16 2010-10-13 コリア インスティチュート オブ ジオサイエンス アンド ミネラル リソースィーズ（キガム） Sample storage device
WO2015046237A1 (en) * 2013-09-26 2015-04-02 東京エレクトロン株式会社 Cell culturing apparatus and cell culturing system

Also Published As

Publication number Publication date
JP4236687B2 (en) 2009-03-11

Similar Documents

Publication Publication Date Title
JP4004310B2 (en) Incubator
US6129428A (en) Storage device for objects, storage station and air-conditioned cabinet
JP5450326B2 (en) Cryogenic storage system
JPWO2016148254A1 (en) Cryopreservation device
JP6133070B2 (en) Cryogenic storage system
SG163496A1 (en) Device for supporting a plurality of flexible containers for liquid
JP2008030914A (en) Clean stocker and article storage method
JP6147221B2 (en) Low temperature storage and low temperature storage system
US20090053020A1 (en) Substrate processing apparatus
BR102013029819A2 (en) ROBOT SYSTEM, ROBOT HAND, ROBOT, AND A ROBOT CONTROL METHOD
US20240083615A1 (en) System for transporting loose sterile closure elements
EP3006867B1 (en) Low-temperature storage device with rotating lock chamber
JP2011051770A (en) Cassette carrying device
JP4236687B2 (en) Incubator
JP2018000163A (en) Incubator apparatus for isolators
JP4348520B2 (en) Transport system
JP2008184314A (en) Picking system
WO2020024841A1 (en) Ware management device
JP2016117570A (en) Storage system
JP2017065912A (en) Article storage system
JP2017063712A (en) Plant culture apparatus
US20120107082A1 (en) Climate Cabinet Having Multiple Intake and Output Stations
JP4289093B2 (en) Tray transport system
CN111573091B (en) Article storage facility
JP6928300B1 (en) Cold storage system

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20070620

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20070724

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070925

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20081118

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20081216

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20111226

Year of fee payment: 3

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121226

Year of fee payment: 4

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121226

Year of fee payment: 4

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313111

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121226

Year of fee payment: 4

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121226

Year of fee payment: 4

FPAY Renewal fee payment (prs date is renewal date of database)

Free format text: PAYMENT UNTIL: 20131226

Year of fee payment: 5

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

LAPS Cancellation because of no payment of annual fees