JP2007192654A - Measuring device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To suppress reduction of measurement accuracy due to flow of compound solution in a flow channel in a measuring device. <P>SOLUTION: A measuring section 101 is prepared which comprises a substrate block 50 having a measuring plane 51 to which a ligand Ta is fixed and a flow channel block 60 arranged on the measuring plane 51 for constituting the flow channel 61 for guiding analyte solution Ky onto the ligand Ta. When an injecting section 71 injects the analyte solution Ky into the flow channel 61 from a flow channel inlet 62 disposed on the upstream side of the flow channel 61, and a binding state of the ligand Ta and analyte K in the analyte solution Ky injected into the flow channel 61 is measured, part of the analyte solutions Ky injected into the flow channel 61 is sucked and taken out of the flow channel inlet 62 by an inlet sucking section 80. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a measuring apparatus, and more particularly to a measuring apparatus that measures the binding state between an analyte and a ligand in a flow path through which an analyte solution passes.

  2. Description of the Related Art Conventionally, a measuring apparatus using total reflection attenuation for measuring a binding state between a protein and a compound is known. The measuring device using surface plasmon resonance, which is an example of the measuring device using total reflection attenuation, includes a dielectric block having a measuring surface on which a protein serving as a ligand is fixed, and a protein block fixed on the measuring surface. There is known a compound solution in which a compound serving as an analyte is dissolved, that is, a measurement unit including a flow path block in which a flow path for passing an analyte solution is formed (see Patent Document 1). The measurement surface is a total reflection surface for measuring the total reflection attenuation angle, and a metal film is laminated on the dielectric block.

  In the measurement apparatus using the surface plasmon resonance, the compound solution is injected through the injection pipe from the flow channel inlet arranged on the upstream side of the flow channel formed in the measurement unit, and the compound solution and the measurement surface are fixed. The binding state between the protein and the compound is measured by contacting the protein and measuring the change in the total reflection attenuation angle on the measurement surface.

  More specifically, after starting the measurement of the total reflection attenuation angle, a compound solution of several times the capacity of the channel is injected from the channel inlet, and then the compound solution injection is stopped to enter the channel. After a predetermined residence time has elapsed with the compound solution being retained, the measurement of the total reflection attenuation angle is terminated. Based on the change in the total reflection attenuation angle from the start of measurement to the end of measurement, the binding state between the protein and the compound can be measured.

  A flow path outlet is provided on the downstream side of the flow path, and the portion of the compound solution injected from the flow path inlet that is discharged from the flow path outlet is sucked and taken out through the suction pipe.

  Furthermore, a measuring apparatus in which the volume of the flow path is 20 microliters or less and the maximum diameter of the flow path is 1 mm or less has been studied so as not to unnecessarily increase the injection amount of the compound solution.

  In addition, since the measurement of the change amount of the total reflection attenuation angle requires high accuracy, vibration to the measurement unit during measurement is suppressed. For example, when the injection of the compound solution is stopped and the compound solution is retained in the channel, if the compound solution in the channel flows due to vibration or the like, the contact state between the compound solution and the protein changes, so that The amount of change in the reflection attenuation angle varies. For this reason, the measuring apparatus as described above is generally provided with anti-vibration measures.

JP 2005-257522 A

  By the way, after the injection pipe arranged at the channel inlet and the suction pipe arranged at the channel outlet stop the injection of the compound solution from the channel inlet, both are separated from the channel block and are in a standby state. It becomes. Therefore, the compound solution in the channel does not receive any action from the outside of the channel block during the lapse of the predetermined residence time in which the compound solution is retained in the channel.

  However, during the predetermined residence time, the liquid level of the compound solution at the channel inlet gradually rises, and the compound solution may overflow from the channel inlet.

  If the liquid surface position fluctuates during the measurement as described above, the compound solution contained in the flow path flows and the contact state between the compound and the protein changes, so the total reflection attenuation angle fluctuates. There is a problem that the measurement accuracy of the binding state of the compound and the compound decreases. Further, this problem becomes more serious when the diameter of the channel is reduced.

  The decrease in the measurement accuracy of the binding state between the protein and the compound is not limited to the case where the total reflection attenuation angle is measured using a dielectric block, but the analyte and the substrate in the analyte solution injected into the channel. This is a problem that generally arises when measuring the binding state with a ligand immobilized on a block.

  The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a measuring apparatus capable of suppressing a decrease in measurement accuracy due to the flow of an analyte solution in a flow path.

  The measuring apparatus of the present invention includes a base block having a measuring surface on which a ligand is fixed, and a channel block disposed on the measuring surface to form a channel for guiding an analyte solution in which the analyte is dissolved on the ligand. And an injection means for injecting the analyte solution into the flow path from the flow path inlet disposed on the upstream side of the flow path formed in the measurement section, and was injected into the flow path In the measuring apparatus for measuring the binding state between the analyte in the analyte solution and the ligand, an inlet suction means for sucking and extracting a part of the analyte solution injected into the channel from the channel inlet is provided. It is characterized by this.

  The injection means can also serve as an inlet suction means.

  When the measuring device is provided with an outlet suction means for sucking and taking out a part of the analyte solution injected into the channel from the channel outlet arranged on the downstream side of the channel, this outlet The suction means can also serve as the inlet suction means.

  The ligand means a substance fixed to the base block and specifically binds to a specific substance.

  The analyte refers to a substance that binds to the ligand.

  The measurement surface may be a surface that totally reflects incident light for measurement, for example.

  The base block is transparent to incident light for measurement, and can be a dielectric block made of a glass member, a resin member, or the like.

  The measurement apparatus includes a dielectric block as a base block, and measures the total reflection attenuation angle (total reflection attenuation angle) generated in the light that is incident on the measurement surface of the dielectric block on which the ligand is fixed and is totally reflected. It can be set as the measuring apparatus using reflection attenuation. The total reflection attenuation may be caused by surface plasmon resonance.

  Since the measuring apparatus of the present invention is provided with the inlet suction means for sucking out and extracting a part of the analyte solution injected into the flow path from the flow path inlet, the liquid level position of the analyte solution in the flow path A decrease in measurement accuracy due to fluctuations can be suppressed.

  That is, the inlet suction means can suck a part of the analyte solution in the flow path from the flow path inlet and take out a part or all of the fluctuation of the liquid level position in advance. The effect of varying can be suppressed. As a result, it is possible to reduce the fluctuation of the liquid level position of the analyte solution in the flow path, for example, due to a change in the contact state between the ligand and the analyte due to the flow of the analyte solution during the predetermined residence time. A decrease in measurement accuracy can be suppressed. In addition, as said effect | action, the effect | action etc. which change a liquid level position by drawing in the analyte solution in the flow path near the flow path inlet by capillary action etc. can be mentioned, for example.

  Further, if the injection means also serves as an inlet suction means, a part of the analyte solution in the flow path can be sucked and taken out from the flow path inlet more easily, and the measurement accuracy can be further reduced. It can be easily suppressed.

  Further, the measuring device further comprises an outlet suction means for sucking and taking out a part of the analyte solution injected into the flow path from the flow path outlet disposed on the downstream side of the flow path. If it also serves as the suction means, it is possible to more easily suppress the decrease in the measurement accuracy for the same reason as described above.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a conceptual diagram showing a schematic configuration of a measuring apparatus according to an embodiment of the present invention, and FIG. 2 is a perspective view showing a measuring unit including a dielectric block and a flow path block. Note that the flow path block shown in FIG. 1 shows a cross section cut along a plane parallel to the XY plane in the drawing orthogonal to the measurement surface and passing through the flow path outlet.

  As shown in FIGS. 1 and 2, a measurement apparatus 100 using total reflection attenuation, which is an example of the measurement apparatus of the present invention, is an example of a substrate block having a measurement surface 51 on which a protein Ta as a ligand is fixed. A flow path block arranged on the measurement surface 51 in order to constitute a dielectric block 50 and an analyte solution in which the compound K as an analyte is dissolved, that is, a flow path 61 for guiding the compound solution Ky onto the protein Ta. The measurement unit 101 including 60 and the injection unit 71 for injecting the compound solution Ky into the channel 61 from the channel inlet 62 disposed on the upstream side of the channel 61 were injected into the channel 61. The binding state between the compound K in the compound solution Ky and the protein Ta fixed to the measurement surface 51 is measured.

  The measurement surface 51 of the dielectric block 50 is a surface that totally reflects the incident light beam Le when measuring the total reflection attenuation angle. The protein Ta is fixed on the measurement surface 51 of the dielectric block 50 by laminating the gold film 52 and the protein Ta in this order on the measurement surface 51 of the dielectric block 50.

  The capacity of the channel 61 is 10 microliters, and the maximum area of the cross section of the channel 61 perpendicular to the direction in which the channel 61 extends is 0.5 mm.

  The dielectric block 50 can be formed of a silicon member or a polyethylene member. The flow path block 60 can be formed of a resin member, a glass member, or the like.

  Further, the measuring apparatus 100 includes an outlet suction unit 75 that sucks and takes out a part of the compound solution Ky injected into the channel 61 from a channel outlet 63 arranged on the downstream side of the channel 61, and a channel. And an inlet suction section 80 for sucking and taking out a part of the compound solution Ky injected into 61 from the flow path inlet 62.

  Further, the total reflection attenuation angle measurement unit 102 causes the light beam Le to enter the measurement surface 51 through the dielectric block 50, measures the light beam Le totally reflected by the measurement surface 51, and generates the surface on the measurement surface 51. A total reflection attenuation angle corresponding to the plasmon resonance is obtained, and a measurement value indicating a binding state between the compound K and the protein Ta is obtained based on the total reflection attenuation angle.

  More specifically, the total reflection attenuation angle measurement unit 102 includes a laser light source 14 that generates a light beam Le, a collimator lens 15a that converts the light beam Le emitted from the laser light source 14 into parallel light, and the parallel light. A condensing lens 15 b is provided to make the beam Le converge from the side surface 50 L of the dielectric block 50 and to enter the measurement surface 51 of the dielectric block 50.

  Further, the total reflection attenuation angle measurement unit 102 includes a collimating lens 16 that collimates the light beam Le reflected from the measurement surface 51 and emitted from the side surface 50R of the dielectric block 50 in a diverging state. A light receiving unit 17 in which a large number of light receiving elements for detecting the light beam Le are arranged in one direction, and a signal indicating the total reflection attenuation angle detected by the light receiving unit 17 is processed to determine the amount of binding between the protein Ta and the compound K. A signal processing unit 18 to be obtained, and a display unit 19 for displaying a result processed by the signal processing unit 18. One direction in which the light receiving elements are arranged is perpendicular to the propagation direction of the light beam Le totally reflected by the measurement surface 51 and parallel to the XY plane in the drawing.

  The light beam Le incident on the measurement surface 51 includes components that are incident on the measurement surface 51 at various incident angles θ, and the incident angle θ is an angle that is greater than or equal to the total reflection angle. Therefore, the light beam Le totally reflected by the measurement surface 51 includes a component of the dark line Drk indicating total reflection attenuation.

  The control unit 103 controls the operations of the injection unit 71, the outlet suction unit 75, the inlet suction unit 80, the total reflection attenuation angle measurement unit 102, and the like, and the timing of these operations.

  Hereinafter, the operation when acquiring the measurement value indicating the binding state between the compound K and the protein Ta by the measurement apparatus 100 will be described. 3 (a) to 3 (e) are conceptual diagrams showing a state in which a binding state between a compound and a protein is measured by the above-described measuring apparatus. FIG. 4 corresponds to time on the horizontal axis t and total reflection attenuation angle on the vertical axis E It is a figure which shows the value of angle data which changes with progress of time on the coordinate which shows the value of angle data to do. FIGS. 5A and 5B are diagrams showing the behavior of the compound solution in the channel when a part of the compound solution Ky in the channel is not sucked out from the channel inlet.

  3A to 3E show the above-described measurement states (steps) in this order in time series. FIG. 5 (a) is a diagram showing how the liquid level of the compound solution in the flow path rises when the compound solution in the flow path is not taken out from the flow path inlet, and FIG. 5b shows the compound solution in the flow path. It is a figure which shows a mode that the compound solution in a flow path overflows from a flow path when it does not take out from a flow path inlet.

  As shown in FIG. 3A, in the initial state, a buffer solution By which is not bound to the protein Ta fixed on the measurement surface 51, eg, a phosphate buffer solution (PBS), is filled in the channel 61. Yes.

  In the initial state, the injection pipe 72 included in the injection section 71, the outlet suction pipe 76 included in the outlet suction section 75, and the inlet suction pipe 81 included in the inlet suction section 80 are respectively connected to the flow path inlet 62 and the flow path outlet 63. Waiting at a position away from That is, the respective parts are kept on standby so as not to affect the measurement of the total reflection attenuation angle with respect to the buffer solution By.

  In this state, the total reflection attenuation angle measurement unit 102 starts measuring angle data indicating the total reflection attenuation angle.

  As shown in FIG. 4, the value of the angle data Da (to-t1) indicating the total reflection attenuation angle from the time to when the state is maintained to the time t1 after the elapse of a predetermined time indicates a constant value α1. The value α1 of the angle data Da acquired at this time is a measurement reference point.

  At the time t1, as shown in FIG. 3B, the distal end portion 72A of the injection pipe 72 is positioned at the injection position of the flow path inlet 62, and the front end section 76B of the outlet suction pipe 76 is sucked by the flow path outlet 63. To position. Then, the injection part 71 discharges the compound solution Ky from the distal end part 72 </ b> A of the injection tube 72, and injects the compound solution Ky into the flow path 61 through the flow path inlet 62. At the same time, the outlet suction part 75 sucks and takes out the buffer solution By and the compound solution Ky discharged from the flow path outlet 63 from the distal end part 76B of the outlet suction pipe 76.

  In addition, the flow path block 60 is provided with a tapered portion 64 formed so that an opening is widened from the flow path inlet 62 toward the outside, and the side surface of the injection pipe 72 is in close contact with the tapered portion 64. Then, the compound solution Ky is discharged. Thereby, leakage of the compound solution Ky through the flow path inlet 62 to the outside is suppressed.

  The flow path block 60 is provided with a drainage reservoir 65 for storing the buffer solution By and the compound solution Ky discharged from the flow path outlet 63. The drainage reservoir 65 is a concave part formed by removing a part of the upper side of the flow path block 60, and the flow path outlet 63 is located at the center of the bottom of the concave part. The outlet suction part 75 sucks and extracts the buffer solution By and the compound solution Ky accumulated in the drainage reservoir 65 from the tip part 76B.

  The injection part 71 stops the injection after injecting the compound solution Ky of 9 times the capacity of the channel 61 into the channel 61 by time t2. As the injection of the compound solution Ky into the channel 61 is stopped, the outlet suction unit 75 stops the suction of the buffer solution By and the compound solution Ky discharged from the channel outlet 63. Thereby, all the buffer solution By accommodated in the flow path 61 is discharged | emitted.

  As shown in FIG. 4, the value of the angle data Da (t1-t2) indicating the total reflection attenuation angle increases rapidly between time t1 and time t2. This abrupt change is mainly caused by the fact that the buffer solution By contained in the flow path 61 is replaced with the compound solution Ky having a higher refractive index than the buffer solution By. The change amount of the total reflection attenuation angle due to the binding between the compound K in the compound solution Ky injected into 61 and the protein Ta on the measurement surface 51 is also included.

  As shown in FIG. 3C, when the injection of the compound solution Ky into the flow path 61 is stopped at the time t2, the injection pipe 72 and the outlet suction pipe 76 are immediately connected to the flow path inlet 62 and the flow path. It is moved to a position away from the road exit 63. 3D, the distal end portion 81C of the inlet suction pipe 81 is positioned at the flow path inlet 62, and the inlet suction section 80 transfers a part of the compound solution Ky in the flow path 61 to the flow path inlet. Aspirate from 62 and remove.

  Thereafter, as shown in FIG. 3E, the inlet suction pipe 81 is separated from the flow path inlet 62 and returned to its original position. Then, the compound solution Ky until the time t3 when the combined state of the compound K and the protein Ta in the channel 61 is saturated is retained in the channel 61. The inlet suction pipe 81 and the outlet suction pipe 76 are arranged so as not to affect the measurement of the total reflection attenuation angle for the compound solution Ky during the predetermined residence time in which the compound solution Ky is retained in the flow path 61. , And the injection pipe 72 are kept waiting at positions separated from the flow path inlet 62 and the flow path outlet 63, respectively.

  As shown in FIG. 4, the value of the angle data Da (t2-t3) indicating the total reflection attenuation angle gradually increases during the elapse of the predetermined residence time from time t2 to time t3. This change is mainly caused by the binding between the protein Ta fixed to the measurement surface 51 and the compound solution Ky. The value of the angle data Da (t3) indicating the total reflection attenuation angle at time t3 when the binding state between the compound K and the protein Ta is saturated is βm.

  Here, a part of the compound solution Ky in the channel 61 is sucked and taken out, so that the compound in the channel 61 in the vicinity of the channel inlet 62 while the compound solution Ky is retained in the channel 61 is obtained. An increase in the liquid level position of the solution Ky can be suppressed.

  That is, when a part of the compound solution Ky in the flow path 61 is not sucked and taken out from the flow path inlet 62 by the inlet suction part 80 at the time t2, the flow as shown in FIG. The liquid level position of the compound solution Ky in the flow path 61 or the taper portion 64 in the vicinity of the path entrance 62 rises. Furthermore, as shown in FIG. 5B, the compound solution Ky may overflow from the tapered portion 64. As described above, when the compound solution Ky flows in the flow path 61, the contact state between the compound K in the compound solution K and the protein Ta on the measurement surface 51 changes, and the value of the angle data indicating the total reflection attenuation angle is obtained. Since it fluctuates, the measurement accuracy of the binding amount between the compound K and the protein Ta decreases.

  At the time t3, the buffer solution is injected into the flow path 61. Thereby, when the compound solution Ky and the protein Ta are bonded in the flow path 61, the compound solution Ky is detached from the protein Ta. Further, by time t4, the state in the flow channel 61 and the state of each part are returned to the initial state, that is, the state shown in FIG.

  As shown in FIG. 4, the value of the angle data Da (t3-t4) indicating the total reflection attenuation angle decreases rapidly from time t3 to time t4. This change is mainly caused by the fact that the compound solution Ky staying in the flow path 61 is replaced with the buffer solution By having a lower refractive index than the compound solution Ky. It also includes the change in the total reflection attenuation angle due to the separation of the.

  In order to determine the binding amount between the protein Ta and the compound solution Ky, a reference dielectric block 50A in which only the gold film 52A is arranged on the measurement surface 51A, that is, the dielectric block 50A to which the protein Ta is not fixed is obtained. Prepare the same measurement as above.

  In the measurement using the dielectric block 50A, since the protein Ta is not present, even if the compound solution Ky is injected into the flow path 61, there is nothing that binds to the compound K contained in the compound solution Ky. In addition, there is no combination with the gold film 52A on the measurement surface 51A of the dielectric block 50A.

  Then, as shown in FIG. 4, the total reflection attenuation at time t3 is the same as that when using the dielectric block 50 with the protein Ta fixed, as measured using the dielectric block 50A without the protein Ta fixed. A reference value βr of angle data Db (t3) indicating a corner is obtained. Then, a reference obtained by measurement using the dielectric block 50A to which the protein Ta is not fixed, from an actual measurement value βm which is a value obtained by measurement using the dielectric block 50 to which the protein Ta is fixed. The angle difference data δ (t3) obtained by subtracting the value βr is a value corresponding to the binding amount between the protein Ta and the compound K.

  The angle data Da (t3) and the reference angle data Db (t3) detected by the light receiving unit 17 included in the total reflection attenuation angle measuring unit 102 are input to the signal processing unit 18, and the signal processing unit 18 Angular difference data δ (t3) is obtained. Further, the signal processing unit 18 converts the value δ into a binding amount between the protein Ta and the compound K, and obtains a binding amount between the protein Ta and the compound K in the flow path 61 of the dielectric block 50. The coupling amount obtained as described above is displayed on the display unit 19.

  The actual measurement value and the reference value can also be acquired as follows. FIG. 6 is a diagram illustrating a measurement unit in which an actual measurement region and a reference region are provided in one flow path.

  As shown in FIG. 6, a measurement unit 101A that enables simultaneous measurement of an actual measurement value and a reference value includes a dielectric block 50A and a flow path block 60A. An actual measurement area Jm for measurement, in which a gold film 52A and a protein Ta are stacked and fixed in this order on the measurement surface 51A in one flow path 61A provided on the measurement surface 51A of the dielectric block 50A. Two regions, a reference region Jr for reference, in which the gold film 52A is fixed and the protein Ta is not fixed, are provided on the measurement surface 51A, and the total reflection attenuation angle is measured in the same manner as described above for these two types of regions. It may be performed simultaneously.

  Next, a modified example of the drainage reservoir provided in the flow path block will be described. 7 and 8 are diagrams showing a modification of the drainage reservoir provided in the flow path block.

  As shown in FIG. 7, the drainage reservoir 65M provided in the channel block 60M stores the compound solution Ky discharged from the channel outlet 63M. The drainage reservoir 65M is formed of a concave portion formed by removing a part on the upper surface 69M side of the flow path block 60M, and the flow path outlet 63M is provided so as to protrude from the bottom of the concave shape portion. Yes.

  Of the side surfaces constituting the drainage reservoir 65M, the side surface on the flow channel inlet 62M side is located in the vicinity of the protruding flow channel outlet 63M. The channel inlet 62M is connected to the channel outlet 63M through the channel 61M. Further, the flow path outlet 63M protruding from the bottom of the concave portion is provided at a position lower than the upper surface 69M of the flow path block 60M.

  As shown in FIG. 8, the drainage reservoir 65N provided in the flow path block 60N stores the compound solution Ky discharged from the flow path outlet 63N. The drainage reservoir 65N is composed of a concave portion formed by removing a part on the upper surface 69N side of the flow path block 60N, and the flow path outlet 63N is provided so as to protrude from the bottom of the concave shape portion. Yes. The concave portion has a U-shape when viewed from above (in the direction of arrow Y in the figure), and both upper ends of the U-shape are located on the channel inlet 62N side, and the lower end of the U-shape Is located on the channel outlet 63N side. The flow path inlet 62N is connected to the flow path outlet 63N through the flow path 61N. Further, the flow path outlet 63N protruding from the bottom of the concave portion is provided at a position lower than the upper surface 69N of the flow path block 60N.

  In the above embodiment, after injecting the compound solution Ky into the flow path 61, the tip end portion 81C of the inlet suction pipe 81 is positioned at the flow path inlet 62 so that a part of the compound solution Ky in the flow path 61 flows. Although an example in which the suction is taken out from the passage inlet 62 has been shown, the present invention is not limited to such a case, and any type of the compound solution Ky in the flow passage 61 may be sucked and taken out from the passage inlet 62 by any method. Good. For example, the distal end portion 72A of the injection tube 72 may be positioned at the flow path inlet 62, and a part of the compound solution Ky in the flow path 61 may be sucked and taken out from the flow path inlet 62 by the injection section 71. In this case, the injection part 71 also serves as an inlet suction means. Further, the distal end portion 76B of the outlet suction pipe 76 is positioned at the flow path inlet 62, and a part of the compound solution Ky in the flow path 61 is sucked and taken out from the flow path inlet 62 by the outlet suction section 75. Good. In this case, the outlet suction part 75 also serves as an inlet suction means.

  When the inlet suction means does not serve as the injection means, the inlet suction means may be a needle-like injection tube having a hollow tip instead of a pipette.

  Furthermore, even when neither the outlet suction means nor the inlet suction means serves as the injection means, the inlet suction means may be a needle-like injection tube having a hollow tip instead of a pipette. .

  In the above-described embodiment, the measurement apparatus using total reflection attenuation based on the principle of surface plasmon resonance has been described. However, the above-described method is also applied to a measurement apparatus using total reflection attenuation based on other principles. be able to. For example, the present invention can be applied to a leakage mode measuring device.

  Furthermore, the present invention is not limited to a case where the present invention is applied to a measuring device using a dielectric block and total reflection attenuation. That is, the present invention measures the binding state between the analyte and the ligand without using total reflection attenuation when the binding state between the analyte and the ligand is measured using a non-dielectric block. Can also be applied in case

The conceptual diagram which shows schematic structure of the measuring apparatus by embodiment of this invention The perspective view which shows the measurement part which consists of a dielectric material block and a flow-path block Conceptual diagram showing the state of the first step of measuring the binding state between a compound and protein using a measuring device Conceptual diagram showing the state of the first step of measuring the binding state between a compound and protein using a measuring device Schematic diagram showing the state of the third step of measuring the binding state between a compound and protein using a measuring device Schematic diagram showing the state of the fourth step of measuring the binding state of the compound and protein with the measuring device Schematic diagram showing the state of the fifth step of measuring the binding state of the compound and protein with the measuring device The figure which shows the value of angle data which changes with the passage of time The figure which shows a mode that the liquid level of the compound solution in a flow path rises when the compound solution in a flow path is not taken out from the flow path inlet. The figure which shows a mode that the compound solution in a flow path overflows from a flow path when the compound solution in a flow path is not taken out from the flow path inlet It is a perspective view which shows the measurement part which provided the measurement area | region and the reference area | region in one flow path. The figure which shows the modification of the drainage reservoir provided in the flow-path block The figure which shows the modification of the drainage reservoir provided in the flow-path block

Explanation of symbols

50 Dielectric block 51 Measurement surface 60 Flow path block 61 Flow path 62 Flow path inlet 71 Injection section 80 Inlet suction section 101 Measurement section Ta ligand K analyte Ky analyte solution

Claims (3)

A measurement unit comprising a base block having a measurement surface on which a ligand is fixed, and a flow channel block disposed on the measurement surface to form a flow channel for guiding an analyte solution in which the analyte is dissolved on the ligand;
Injecting means for injecting the analyte solution into the channel from the channel inlet disposed on the upstream side of the channel formed in the measurement unit,
In the measurement apparatus for measuring the binding state between the analyte and the ligand in the analyte solution injected into the flow path,
A measuring apparatus comprising an inlet suction means for sucking and taking out a part of the analyte solution injected into the flow path from the flow path inlet.   The measuring apparatus according to claim 1, wherein the injection means also serves as the inlet suction means.   An outlet suction means for sucking and taking out a part of the analyte solution injected into the flow path from a flow path outlet disposed on the downstream side of the flow path, and the outlet suction means includes the inlet suction means; The measuring apparatus according to claim 1, wherein the measuring apparatus is also used.

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