JP2007117830A - Ultarsonic micro plate agitation and defoaming apparatus - Google Patents

Ultarsonic micro plate agitation and defoaming apparatus Download PDF

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JP2007117830A
JP2007117830A JP2005311125A JP2005311125A JP2007117830A JP 2007117830 A JP2007117830 A JP 2007117830A JP 2005311125 A JP2005311125 A JP 2005311125A JP 2005311125 A JP2005311125 A JP 2005311125A JP 2007117830 A JP2007117830 A JP 2007117830A
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microplate
ultrasonic
horn
agitation
defoaming
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Toshiaki Miyamoto
年昭 宮本
Takeharu Nakahira
竹春 仲平
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Honda Electronics Co Ltd
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Honda Electronics Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam

Abstract

<P>PROBLEM TO BE SOLVED: To resolve the problem that an agitation and defoaming apparatus using a conventional motor can not cope with agitation and defoaming in a high-density microplate. <P>SOLUTION: The ultrasonic micro plate agitation and defoaming apparatus is structured of a bolt-tightening Langevin-type transducer 1 consisting of a piezoelectric crystal transducer 1c put between a metallic block 1a, 1b of both ends, wherein an oscillator 2 is connected to the piezoelectric crystal transducer 1c of the bolt-tightening Langevin-type transducer 1, a booster 3 is fixed to the ultrasonic-wave generating side of the bolt tightening Langevin-type transducer 1, a horn 4 is fixed to the end part of the booster 3, a microplate connecting part 5 is installed to the end part of the horn 4, and the microplate 6 is contacted to the microplate connecting part 5. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

本発明は、マイクロプレートに超音波を照射し、各Ｗｅｌｌの撹拌・脱泡を高速に行う超音波マイクロプレート撹拌・脱泡装置に関するものである。   The present invention relates to an ultrasonic microplate stirring / defoaming apparatus that irradiates a microplate with ultrasonic waves and performs stirring / defoaming of each well at high speed.

一般に、細胞培養液の撹拌や、この細胞培養液に試薬を混合したり、大量にスクリーニングする場合において、これらを高い信頼性で、精度よく実施するには、従来の少ないＷｅｌｌマイクロプレートより、高密度のマイクロプレートを用いて、高速で、高感度な測定を行わなければならない。   In general, when stirring a cell culture solution, mixing a reagent with this cell culture solution, or performing screening in large quantities, it is more reliable than the conventional well microplates to carry out these operations with high reliability and accuracy. High-speed, high-sensitivity measurements must be performed using a density microplate.

しかしながら、従来のモータを用いた撹拌・脱泡装置では、高密度なマイクロプレートでの撹拌、脱泡に対応することができないという問題があった。   However, the conventional stirring / defoaming apparatus using a motor has a problem that it cannot cope with stirring and defoaming with a high-density microplate.

解決しようとする問題点は、従来のモータを用いた撹拌・脱泡装置では、高密度なマイクロプレートでの撹拌、脱泡に対応することができないという問題があった。   The problem to be solved is that a conventional stirring / defoaming apparatus using a motor cannot cope with stirring and defoaming in a high-density microplate.

本発明では、超音波振動子は発信器から発信出力を印加することにより超音波振動し、ホーンは超音波振動子の端部に固着され、前記超音波振動子からの超音波を集中させ、マイクロプレート接触部はホーンの端部に装着され、マイクロプレートはマイクロプレート接触部に装着され、マイクロプレート接触部に装着されたマイクロプレートの撹拌、脱泡を行うものである。   In the present invention, the ultrasonic vibrator vibrates ultrasonically by applying a transmission output from the transmitter, the horn is fixed to the end of the ultrasonic vibrator, concentrates the ultrasonic waves from the ultrasonic vibrator, The microplate contact portion is attached to the end of the horn, the microplate is attached to the microplate contact portion, and the microplate attached to the microplate contact portion is agitated and degassed.

本発明の超音波マイクロプレート撹拌・脱泡装置では、細胞培養液の撹拌、各種生化学アセッテイ用液の撹拌、ビーズ・溶剤、試薬の混合などのように、高密度マイクロプレートの撹拌・脱泡による科学物のハイスループット、スクリーニング（ＨＷＳ）が高速で、高い信頼性を持って行うことができるという利点がある。   In the ultrasonic microplate agitation / deaeration apparatus of the present invention, agitation / deaeration of high-density microplates such as agitation of cell culture solution, agitation of various biochemical asset liquids, mixing of beads / solvents, reagents, etc. There is an advantage that high throughput and screening (HWS) of scientific materials can be performed at high speed and with high reliability.

本発明では、マイクロプレートの撹拌・脱泡を超音波振動子の超音波発生面に装着したホーンに、マイクロプレートを装着することにより、有効に、高速に行うことができた。   In the present invention, agitation and defoaming of the microplate can be effectively performed at high speed by attaching the microplate to the horn attached to the ultrasonic wave generation surface of the ultrasonic vibrator.

図１は本発明の実施例の超音波マイクロプレート撹拌・脱泡装置の側面図で、圧電体振動子１ｃを両端の金属ブロック１ａ、１ｂで挟持し、ボルト１ｄを金属ブロック１ｂの中心孔に挿入して端部からでめ付けたボルト締めランジュバン型振動子１が構成され、このボルト締めランジュバン型振動子１の圧電体振動子１ｃに発信器２が接続され、又、ボルト締めランジュバン型振動子１の超音波発生面にブースター３が固着され、ブースター３の端部にホーン４が固着され、ホーン４の端部にマイクロプレート接触部５が装着され、このマイクロプレート接触部５にマイクロプレート６が接触されている。   FIG. 1 is a side view of an ultrasonic microplate agitation / defoaming apparatus according to an embodiment of the present invention. A piezoelectric vibrator 1c is sandwiched between metal blocks 1a and 1b at both ends, and a bolt 1d is placed in a central hole of the metal block 1b. A bolt-clamped Langevin type vibrator 1 that is inserted and fastened from the end is configured, a transmitter 2 is connected to the piezoelectric vibrator 1c of the bolt-clamped Langevin type vibrator 1, and a bolt-clamped Langevin type vibration is also provided. A booster 3 is fixed to the ultrasonic wave generation surface of the child 1, a horn 4 is fixed to the end of the booster 3, and a microplate contact portion 5 is attached to the end of the horn 4, and the microplate contact portion 5 is attached to the microplate. 6 is in contact.

このように構成された本実施例の超音波マイクロプレート撹拌・脱泡装置では、発信器２からボルト締めランジュバン型振動子１の圧電体振動子１ｃに発振出力が印加されると、ブースター３を超音波振動し、さらに、ホーン４によって超音波振動を先端に集中させることにより、ホーン４の端部に装着したマイクロプレート接触部５を介してマイクロプレート６が超音波振動され、細胞培養液の撹拌、各種生化学アセッテイ用液の撹拌、ビーズ・溶剤、試薬の混合などのように、高密度マイクロプレートの撹拌・脱泡による科学物のハイスループット、スクリーニング（ＨＷＳ）が高速で、高い信頼性を持って行うことができる。   In the ultrasonic microplate agitation / defoaming device of the present embodiment configured as described above, when an oscillation output is applied from the transmitter 2 to the piezoelectric vibrator 1c of the bolted Langevin vibrator 1, the booster 3 is turned on. The microplate 6 is ultrasonically vibrated via the microplate contact portion 5 attached to the end of the horn 4 by ultrasonically vibrating and further concentrating the ultrasonic vibration on the tip by the horn 4. High-throughput, high-throughput screening (HWS) of scientific materials using high-density microplate agitation and defoaming, such as agitation, agitation of various biochemical assets, mixing of beads / solvents, reagents, etc., high reliability Can be done.

図２は本発明の他の実施例の超音波マイクロプレート撹拌・脱泡装置の側面図で、マイクロプレート６は支持部材７で支持され、この支持部材７の両端にそれぞれホーン４が直列に固着され、ホーン４にそれぞれブースター３が固着され、ブースター３にボルト締めランジュバン型振動子１が固着されている。   FIG. 2 is a side view of an ultrasonic microplate agitation / defoaming apparatus according to another embodiment of the present invention. The microplate 6 is supported by a support member 7, and horns 4 are fixed in series to both ends of the support member 7, respectively. The booster 3 is fixed to the horn 4, and the bolted Langevin type vibrator 1 is fixed to the booster 3.

このように構成された本実施例の超音波マイクロプレート撹拌・脱泡装置では、発信器２からそれぞれボルト締めランジュバン型振動子１の圧電体振動子１ｃに発振出力が印加されると、それぞれのブースター３を超音波振動し、さらに、それぞれのホーン４によって超音波振動を先端に集中させることにより、ホーン４の端部に装着した支持部材７を介してマイクロプレート６が超音波振動され、細胞培養液の撹拌、各種生化学アセッテイ用液の撹拌、ビーズ・溶剤、試薬の混合などのように、高密度マイクロプレートの撹拌・脱泡による科学物のハイスループット、スクリーニング（ＨＷＳ）が高速で、高い信頼性を持って行うことができる。   In the ultrasonic microplate agitation / defoaming device of this embodiment configured as described above, when an oscillation output is applied from the transmitter 2 to the piezoelectric body vibrator 1c of the bolted Langevin vibrator 1, respectively, The booster 3 is ultrasonically vibrated, and the ultrasonic vibration is concentrated on the tip by each horn 4, so that the microplate 6 is ultrasonically vibrated via the support member 7 attached to the end of the horn 4, and the cell High-throughput and high-throughput screening (HWS) of scientific materials by stirring and degassing of high-density microplates, such as stirring of culture fluid, stirring of various biochemical assets, mixing of beads / solvents, reagents, etc. It can be done with high reliability.

図３は本発明のさらに他の実施例の超音波マイクロプレート撹拌・脱泡装置の側面図で、マイクロプレート６は受台材８で支持され、この受台８の両端はそれぞれホーン４に固着された受け部４ａで支持され、ホーン４は縦に並列に固着され、ホーン４にそれぞれブースター３が固着され、ブースター３にボルト締めランジュバン型振動子１が固着されている。   FIG. 3 is a side view of an ultrasonic microplate agitation / defoaming apparatus according to still another embodiment of the present invention. The microplate 6 is supported by a cradle material 8, and both ends of the cradle 8 are fixed to the horn 4. The horns 4 are fixed vertically and in parallel, the boosters 3 are fixed to the horns 4, and the bolted Langevin vibrator 1 is fixed to the boosters 3.

このように構成された本実施例の超音波マイクロプレート撹拌・脱泡装置では、発信器２からそれぞれボルト締めランジュバン型振動子１の圧電体振動子１ｃに発振出力が印加されると、それぞれのブースター３を超音波振動し、又、それぞれのホーン４によって超音波振動を受け部４ａを介して受台８に集中させ、さらに、受台８を介してマイクロプレート６が超音波振動され、細胞培養液の撹拌、各種生化学アセッテイ用液の撹拌、ビーズ・溶剤、試薬の混合などのように、高密度マイクロプレートの撹拌・脱泡による科学物のハイスループット、スクリーニング（ＨＷＳ）が高速で、高い信頼性を持って行うことができる。   In the ultrasonic microplate agitation / defoaming device of this embodiment configured as described above, when an oscillation output is applied from the transmitter 2 to the piezoelectric body vibrator 1c of the bolted Langevin vibrator 1, respectively, The booster 3 is ultrasonically vibrated, and the ultrasonic vibration is received by the respective horns 4 and concentrated on the receiving base 8 via the portion 4a. Further, the microplate 6 is ultrasonically vibrated via the receiving base 8, and the cells High-throughput and high-throughput screening (HWS) of scientific materials by stirring and degassing of high-density microplates, such as stirring of culture fluid, stirring of various biochemical assets, mixing of beads / solvents, reagents, etc. It can be done with high reliability.

なお、上記実施例において、ボルト締めランジュバン型振動子１にブースター３を介してホーン４を固着したが、ブースター３を省いても同様に超音波振動子１の超音波振動をホーンに伝達することができ、又、図２によっておいて、一方のホーン４をスライドするように支持部材７を構成してもよいし、さらに、図３において、ホーン４をスライドするように受台８を構成してもよい。   In the above embodiment, the horn 4 is fixed to the bolt-clamped Langevin type vibrator 1 via the booster 3. However, even if the booster 3 is omitted, the ultrasonic vibration of the ultrasonic vibrator 1 is transmitted to the horn. In FIG. 2, the support member 7 may be configured to slide one of the horns 4, and the cradle 8 is configured to slide the horn 4 in FIG. May be.

図１は本発明の実施例の超音波マイクロプレート撹拌・脱泡装置の側面図である。FIG. 1 is a side view of an ultrasonic microplate stirring / defoaming apparatus according to an embodiment of the present invention. 図２は本発明の他の実施例の超音波マイクロプレート撹拌・脱泡装置の側面図である。FIG. 2 is a side view of an ultrasonic microplate stirring / defoaming apparatus according to another embodiment of the present invention. 図２は本発明のさらに他の実施例の超音波マイクロプレート撹拌・脱泡装置の側面図である。FIG. 2 is a side view of an ultrasonic microplate stirring / defoaming apparatus according to still another embodiment of the present invention.

符号の説明Explanation of symbols

１ ボルト締めランジュバン型振動子
２ 発信器
３ ブースター
４ ホーン
５ マイクロプレート接触部
６ マイクロプレート
７ 支持部材
８ 受台 DESCRIPTION OF SYMBOLS 1 Bolt tightening Langevin type vibrator 2 Transmitter 3 Booster 4 Horn 5 Microplate contact part 6 Microplate 7 Support member 8 Base

Claims (7)

発信器から発信出力を印加することにより超音波振動する超音波振動子と、該超音波振動子の端部に固着され、前記超音波振動子からの超音波を集中させるホーンと、該ホーンの端部に装着されたマイクロプレート接触部と、該マイクロプレート接触部に装着されたマイクロプレートとからなり、前記マイクロプレート接触部に装着された前記マイクロプレートの撹拌、脱泡を行うことを特徴とする超音波マイクロプレートの撹拌・脱泡装置。   An ultrasonic transducer that vibrates ultrasonically by applying a transmission output from a transmitter; a horn that is fixed to an end of the ultrasonic transducer and that concentrates the ultrasonic waves from the ultrasonic transducer; and It comprises a microplate contact portion attached to an end portion and a microplate attached to the microplate contact portion, wherein the microplate attached to the microplate contact portion is stirred and degassed. Ultrasonic microplate stirring and defoaming device. 前記超音波振動子にブースターを介してホーンを装着することを特徴とする請求項１記載の超音波マイクロプレートの撹拌・脱泡装置。   The ultrasonic microplate stirring / defoaming apparatus according to claim 1, wherein a horn is attached to the ultrasonic vibrator via a booster. 発信器から発信出力が印加された超音波振動する２つの超音波振動子と、該２つの超音波振動子のそれぞれの端部に装着され、前記超音波振動子からの超音波をそれぞれ集中させるホーンと、該ホーンの間に装着されたマイクロプレートとからなることを特徴とする超音波マイクロプレートの撹拌・脱泡装置。 Two ultrasonic transducers that vibrate ultrasonically to which a transmission output is applied from a transmitter, and attached to the respective ends of the two ultrasonic transducers, concentrate the ultrasonic waves from the ultrasonic transducers, respectively. An ultrasonic microplate stirring and defoaming device comprising a horn and a microplate mounted between the horns. 前記それぞれの超音波振動子にブースターを介してそれぞれホーンを装着することを特徴とする請求項３記載の超音波マイクロプレートの撹拌・脱泡装置。   4. The ultrasonic microplate agitation / defoaming device according to claim 3, wherein a horn is attached to each of the ultrasonic transducers via a booster. 前記ホーンを対向して装着し、前記ホーンの間に前記マイクロプレートを装着することを特徴する請求項３記載の超音波マイクロプレートの撹拌・脱泡装置。   4. The ultrasonic microplate agitation / defoaming apparatus according to claim 3, wherein the horns are mounted facing each other, and the microplate is mounted between the horns. 前記ホーンが並列になるように前記マイクロプレート装着することを特徴とする請求項３記載の超音波マイクロプレートの撹拌・脱泡装置。   4. The ultrasonic microplate stirring / defoaming apparatus according to claim 3, wherein the microplate is mounted so that the horns are arranged in parallel. 前記それぞれの超音波振動子にブースターを介してそれぞれホーンを装着することを特徴とする請求項３記載の超音波マイクロプレートの撹拌・脱泡装置。   4. The ultrasonic microplate agitation / defoaming device according to claim 3, wherein a horn is attached to each of the ultrasonic transducers via a booster.
JP2005311125A 2005-10-26 2005-10-26 Ultarsonic micro plate agitation and defoaming apparatus Pending JP2007117830A (en)

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CN104142261A (en) * 2014-07-14 2014-11-12 中国矿业大学 Method and device for rapidly manufacturing pulverized coal polished section by ultrasonic defoaming
JP2015043750A (en) * 2013-08-29 2015-03-12 大日本印刷株式会社 Culture medium-filling method
GB2524759A (en) * 2014-04-01 2015-10-07 Stratec Biomedical Ag Shaker
CN108621298A (en) * 2018-05-15 2018-10-09 常州信息职业技术学院 A kind of novel electron control formula concrete central mix plant
KR20190035575A (en) * 2015-03-26 2019-04-03 메디퓨처스 주식회사 Apparatus for separating live fat stem cell
WO2021262572A1 (en) * 2020-06-25 2021-12-30 Corning Incorporated Ultrasonic devices and methods of use

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015043750A (en) * 2013-08-29 2015-03-12 大日本印刷株式会社 Culture medium-filling method
GB2524759A (en) * 2014-04-01 2015-10-07 Stratec Biomedical Ag Shaker
EP2926893A1 (en) * 2014-04-01 2015-10-07 Stratec Biomedical AG Shaker
US9956590B2 (en) 2014-04-01 2018-05-01 Stratec Biomedical Ag Shaker
CN104142261A (en) * 2014-07-14 2014-11-12 中国矿业大学 Method and device for rapidly manufacturing pulverized coal polished section by ultrasonic defoaming
KR20190035575A (en) * 2015-03-26 2019-04-03 메디퓨처스 주식회사 Apparatus for separating live fat stem cell
KR102136206B1 (en) * 2015-03-26 2020-07-22 메디퓨처스 주식회사 Apparatus for isolating adipose-derived stem cells based on ultrasonic cavitation
CN108621298A (en) * 2018-05-15 2018-10-09 常州信息职业技术学院 A kind of novel electron control formula concrete central mix plant
CN108621298B (en) * 2018-05-15 2020-10-02 常州信息职业技术学院 Novel electronic control type concrete stirring device
WO2021262572A1 (en) * 2020-06-25 2021-12-30 Corning Incorporated Ultrasonic devices and methods of use

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