JP2007097777A - Biological tissue filling body manufacturing device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To perform work from the sampling of medullary fluid or the like to the manufacture of a biological tissue filling body by a simple operation, to reduce burdens on a worker such as a doctor or a nurse and to reduce burdens on a patient as well by shortening work time. <P>SOLUTION: The biological tissue filling body manufacturing device 1 comprises carrying tubes 3a, 6 and 27 whose distal end opening is arranged inside the medullary cavity, a suction device 8 for sucking the inside of the carrying tubes 3a, 6 and 27 to a negative pressure, and a filling material housing part 7 arranged at the middle position of the carrying tubes 3a, 6 and 27 for housing the biological tissue filling material 26. The filling material housing part 7 is provided with a filter 29 for transmitting liquid and blocking the passing of cells on the end part on the side of the suction device 8. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a biological tissue complement manufacturing apparatus that manufactures a biological tissue complement by collecting cells such as stem cells present in a long bone.

Conventionally, a method for regenerating a living tissue based on bone marrow fluid or the like collected from a patient is known (see, for example, Patent Document 1). In addition, a method for producing a biological tissue complement for transplantation into a living body using mesenchymal stem cells collected from a patient is also disclosed (for example, see Patent Document 2).
JP 2002-80377 A JP 2003-320019 A

  However, these methods of Patent Documents 1 and 2 have a disadvantage in that another device is required for collecting bone marrow fluid or the like, and that a biological tissue complement must be manufactured by transferring a container or the like after the collection. is there. At the actual surgical site, collecting bone marrow fluid, transferring containers, and manufacturing biological tissue supplements are complicated and prone to mistakes. There is a problem that it takes a long time.

  The present invention has been made in view of the above-described circumstances, and can perform operations from collection of bone marrow fluid and the like to manufacture of a tissue replacement device with a simple operation, and is a burden on operators such as doctors and nurses. An object of the present invention is to provide a biological tissue complement manufacturing apparatus that can reduce the burden on the patient by reducing the working time.

In order to achieve the above object, the present invention provides the following means.
The present invention relates to a transport tube in which a distal end opening is disposed in a bone marrow cavity, a suction device that sucks the interior of the transport tube to a negative pressure, and a filling material container that is disposed at an intermediate position of the transport tube and houses a biological tissue filling material A living body tissue filling device manufacturing apparatus is provided that includes a filter at the end on the side of the suction device, wherein the filling material accommodating portion transmits a liquid and prevents passage of cells.

  According to the present invention, by disposing the distal end opening of the transport tube in the bone marrow yard and operating the suction device, the transport tube is sucked to a negative pressure, and the bone marrow fluid in the bone marrow cavity passes through the transport tube to the bone marrow cavity. It is taken out. Since the filling material storage part is arranged in the middle of the transport pipe, the bone marrow fluid taken out from the transport pipe is guided into the filling material storage part. Bone marrow fluid or the like guided to the filling material container is separated into liquid and cells by the filter, the liquid passes through the filter and is discharged, and the cells are captured by the filter and remain in the filling material container.

  Since the tissue filling material is accommodated in the filling material container, cells are allowed to adhere to the tissue filling material in the filling material container, thereby producing a tissue filling material. . That is, according to the present invention, it is possible to manufacture a biological tissue filling body in the filling material storage section simply by placing the distal end opening of the transport tube in the bone marrow cavity and operating the suction device.

In the said invention, the said conveyance pipe | tube is good also as providing the substantially cylindrical cutter member which has a cutting blade at the front-end | tip, and the support member which supports this cutter member rotatably about the axis line.
By doing in this way, it becomes possible to perforate cortical bone with a cutting blade by operation of a cutter member, and to arrange a tip opening of a conveyance tube in a bone marrow cavity. Therefore, cortical bone drilling work can also be performed using the biological tissue complement manufacturing apparatus of the present invention, and the work can be simplified.

Moreover, in the said invention, it is good also as the said cutter member being equipped with the cooling device which cools this cutter member.
When the cortical bone is drilled by the cutter member, the cutter member is heated by frictional heat, but since the cutter member is cooled by the operation of the cooling device, the cells taken out through the inside of the cutter member after drilling are maintained in a healthy state. Is done.

In the above invention, the filling material container is configured in a substantially cylindrical shape that is eccentric with respect to the transport tube, and the biological tissue filling material housed therein is disposed around the axis of the filling material container. It is good also as providing the filling material rotation mechanism to rotate.
By doing so, the operation of the filling material rotating mechanism can rotate the living tissue filling material in the filling material container, and the living tissue filling material to which bone marrow fluid conveyed through the conveying tube adheres can be rotated. The position can be changed. Accordingly, it becomes possible to uniformly adhere bone marrow fluid or the like to a relatively large biological tissue filling material, and it is possible to produce a relatively large biological tissue filling body in which cells are uniformly adhered.

Further, in the above invention, the filling material container is configured in a substantially cylindrical shape that is eccentric with respect to the transport tube, and rotatably accommodates a rotor that can be loaded with a plurality of living tissue filling materials therein. It is good also as providing the rotor rotation mechanism which rotates the said rotor around the axis line of this supplementary material accommodating part.
By doing this, a plurality of biological tissue filling materials are loaded into the rotor, and the rotor rotating mechanism is operated, so that the bone marrow fluid conveyed through the conveying tube is sequentially attached to the multiple biological tissue filling materials. Thus, a plurality of biological tissue filling materials can be manufactured by a simple operation.

In the above invention, a cap that is detachably attached to the distal end side of the filling material container is provided at the maximum transverse area of the filling material container, and the transport pipe is attached to the cap. Also good.
By doing in this way, after a biological tissue filling body is manufactured in a filling material accommodating part, a biological tissue filling body can be easily taken out from the filling material accommodating part by removing a cap.

  The present invention also includes a connection tube connected to a through-hole formed in the cortical bone so as to penetrate into the bone marrow cavity, and a spiral groove that is rotatably inserted into the connection tube and has a tip disposed in the bone marrow cavity. There is provided a biological tissue filling body manufacturing apparatus comprising a drill-shaped member having a drill, a drill rotation mechanism for rotating the drill-shaped member, and a filling material container that is connected to a rear end of the connection pipe and houses a living tissue filling material. To do.

  In order to manufacture a biological tissue complement according to the present invention, first, the drill-like member is brought into contact with the cortical bone surface and the drill rotating mechanism is operated, whereby the cortical bone is drilled by the drill-like member, and the tip of the drill-like member is Is placed in the bone marrow cavity. Alternatively, the cortical bone is previously drilled with a through hole, and the tip of the drill-like member is placed in the bone marrow cavity. In this state, since the tip of the spiral groove of the drill-like member is disposed in the bone marrow cavity, by operating the drill rotating mechanism and rotating the drill-like member, the bone marrow fluid in the bone marrow cavity and the bone marrow cavity are rotated along the spiral groove. Cancellous bone is removed from within the bone marrow cavity.

  Since the connecting tube is connected to the through-hole, the extracted bone marrow fluid or the like is transported through the connecting tube to reach the filling material container, and is attached to the biological tissue filling material housed in the filling material container. . Thereby, a biological tissue filling body is manufactured in the biological tissue accommodating part. Therefore, the operator simply presses the tip of the biological tissue filling body manufacturing apparatus according to the present invention against the cortical bone or connects it to the through-hole formed in the cortical bone, so that bone marrow fluid or the like is added to the biological tissue filling material. It is possible to manufacture a biological tissue filling body to which is attached.

In the above invention, the drill-like member disposed in the filling material accommodating portion may be provided with a stirring member for stirring the contents in the filling material accommodating portion by rotating the drill-like member. Good.
By doing so, while the drill-like member is rotated to take out bone marrow fluid and the like, the stirring member is rotated in the filling material container to stir the contents made up of the mixture of bone marrow fluid and the biological tissue filling material. It is possible to produce a biological tissue filling material in which these are uniformly mixed.

Moreover, in the said invention, the cap attached so that attachment or detachment is possible in the largest cross section area of this filling material accommodating part is provided in the front end side of the said filling material accommodating part, and the said connection pipe is attached to the said cap. It is good as well.
By doing in this way, after a biological tissue filling body is manufactured in a filling material accommodating part, a biological tissue filling body can be easily taken out from the filling material accommodating part by removing a cap.

  According to the present invention, it is possible to perform operations from collection of bone marrow fluid and the like to production of a biological tissue complement by simple operations, reducing the burden on workers such as doctors and nurses, and shortening the work time. As a result, the burden on the patient can be reduced.

A biological tissue filling device manufacturing apparatus 1 according to an embodiment of the present invention will be described below with reference to FIGS. 1 and 2.
As shown in FIG. 1, the biological tissue filling body manufacturing apparatus 1 according to the present embodiment is formed in a substantially cylindrical shape, and has a cutter member 3 having a cutting edge 2 at the tip, and rotatably supports the cutter member 3. A support member 4 that rotates, a rotating device 5 that applies a rotational force to the cutter member 3, a flexible tube (conveying tube) 6 that is connected at one end to the rear end of the cutter member 3, and the other end of the flexible tube 6. And a pump (suction device) 8 for depressurizing the inside of the cutter member 3 and a flexible tube 6 connected thereto. The cutter member 3 is provided with a cooling device 9, and the flexible tube 6 is connected to a liquid supply unit 11 that supplies a liquid 10 therein.

  The cutter member 3 includes a tubular portion (conveying tube) 3a formed in a substantially cylindrical tubular shape, and a cutting blade arranged at the distal end of the tubular portion 3a and arranged in the circumferential direction with a sharp point toward the distal end. 2 are provided. Thus, the cutter blade 3 is rotated around its central axis while the tip of the cutting blade 2 is in contact with an object to be drilled (not shown) (for example, the outer surface of the cortical bone of the long bone). The object to be drilled is shaved and the object to be drilled can be drilled.

  The support member 4 is a substantially cylindrical member that is disposed at a midway position in the longitudinal direction of the cutter member 3 so as to surround the cutter member 3, and supports the cutter member 3 rotatably by a bearing 12. is doing. The support member 4 can be operated by an operator by hand.

  The rotating device 5 includes a motor 13 fixed to the support member 4 and gears 14 and 15 fixed to the motor 13 and the cutter member 3 and meshing with each other. By holding the support member 4 and operating the motor 13, the gears 14 and 15 can be rotated to rotate the cutter member 3 around the central axis.

  The cooling device 9 is connected to a container 17 for storing a coolant 16, for example, physiological saline or other chemicals, and a pipe 18 that connects the container 17 and the support member 4 and supplies the coolant 16 to the support member 4. A spiral flow path 19 formed inside the cutter member 3 and a connection flow path 20 connecting the pipe 18 and the flow path 19.

  The connection channel 20 includes a ring-shaped space 22 defined in a sealed state by two seal members 21 that are spaced apart in the axial direction between the cutter member 3 and the support member 4, and the cutter member 3. And a circumferential groove 23 formed on the outer surface and communicating with the ring-shaped space 22. The flow path 19 is connected to the circumferential groove 23.

  Thereby, even in a state where the cutter member 3 is rotated with respect to the support member 4, the coolant 16 is supplied from the container 17 into the flow path 19 through the pipe 18, the ring-shaped space 22, and the circumferential groove 23. The cutter member 3 provided with the flow path 19 can be cooled as a whole. The cooling liquid 16 is supplied from the container 17 by, for example, a pump (not shown).

  In addition, the liquid supply unit 11 includes, for example, a container 24 that stores a liquid 10 such as physiological saline or other chemical solution, and a pipe 25 that connects the container 24 and the flexible tube 6. The liquid 10 stored in the container 24 is supplied into the flexible tube 6 by, for example, gravity or by a pump (not shown), and is supplied into the cutter member 3 connected to the flexible tube 6. It has become.

  The filling material container 7 includes a substantially cylindrical container body 7a for housing the substantially cylindrical body tissue filling material 26, and a cap 7b that is detachably attached so as to close the opening of the container body 7a. ing. The cap 7 b is fixed to one end of the flexible tube 6, and the container body 7 a is fixed to one end of a connection pipe (conveying pipe) 27 connected to the pump 8.

  The cap 7b has a female screw 28b fastened to a male screw 28a provided on the outer peripheral surface of the opening of the container body 7a. The cap 7b is configured to open and close the container body 7a at the maximum cross-sectional area portion of the container body 7a. By removing the cap 7b from the container body 7a, the contents in the container body 7a can be easily taken out. Can be done.

A filter 29 that allows liquid and gas to pass therethrough and prevents passage of cells and the like is provided at the connection port to the connection pipe 27 of the container body 7a.
In the present embodiment, with the opening of the container body 7a closed by the cap 7b, the opening of the flexible tube 6 connected to the cap 7b and the connection pipe 27 of the container body 7a provided with the filter 29 are connected. All the connection ports are arranged at the center position of the longitudinal axis of the container body 7a. Further, the biological tissue filling material 26 accommodated in the filling material container 7 is also formed in a cylindrical shape having a through hole 26a in the center.

  Thereby, when the bone marrow fluid etc. conveyed through the flexible tube 6 enters the filling material container 7 from the opening of the cap 7b, the bone marrow fluid mainly flows in the through hole 26a of the living tissue filling material 26 and flows into the connection port. The filter 29 separates the liquid into cells and the like. Accordingly, the through-holes 26a of the biological tissue filling material 26 are filled with cells and the like contained in the bone marrow fluid, whereby a biological tissue filling material is manufactured.

  In this embodiment, the biological tissue filling material 26 is a cylindrical block made of, for example, a β-tricalcium phosphate porous body. The material of the biological tissue filling material 26 is not limited to this, and any material having biocompatibility can be adopted.

The remaining liquid and gas from which cells and the like are separated from the bone marrow fluid and the like are sucked by the pump 8 and discharged to the outside through the connection pipe 27.
In the figure, reference numeral 30 denotes a seal member that seals between the cutter member 3 and the flexible tube 6.

The operation of the biological tissue filling body manufacturing apparatus 1 according to this embodiment configured as described above will be described below.
In order to manufacture a biological tissue filling body using the biological tissue filling body manufacturing apparatus 1 according to this embodiment, the surface of the long bone is exposed by incising the epidermis, and the support member 4 is operated to operate the cutter member 3. The cutting edge 2 at the tip is brought into contact with the long bone surface. At this time, since the proximal end side of the cutter member 3 is connected to the flexible tube 6, the posture of the cutter member 3 can be freely adjusted by bending the flexible tube 6, and the hole to be drilled in the long bone can be obtained. The cutter member 3 can be aligned with the angle and direction. In this state, the cooling device 9 and the rotating device 5 are operated, and the cutter member 3 is rotated around its central axis in a state where the coolant 16 is circulated in the spiral flow path 19 of the cutter member 3.

  By applying a pressing force toward the distal end of the cutter member 3 to the support member 4, the cutting blade 2 at the distal end of the cutter member 3 is pressed against the long bone, and the cortical bone of the long tubular bone is cut by the rotation of the cutting blade 2. Can be perforated. In this case, when the cutting blade 2 is rotated with respect to the cortical bone, frictional heat is generated due to friction between the cortical bone and the cutting blade 2, but the coolant 16 is supplied to the flow path 19 inside the cutter member 3. Therefore, it is avoided that the cutter member 3 is in a high temperature state.

  When the cutting blade 2 penetrates the cortical bone and is inserted into the bone marrow cavity, the bone marrow fluid or the like stored in the bone marrow cavity flows into the cutter member 3. In this case, since the cutter member 3 is cooled so as not to reach a high temperature state, the bone marrow fluid flowing into the cutter member 3 is prevented from being heated, and the soundness of the mesenchymal stem cells in the bone marrow fluid is prevented. Can be prevented from being damaged by heat. The cancellous bone is also present in the bone marrow cavity, but the cancellous bone is also cut when the cutting blade 2 at the tip of the cutter member 3 comes into contact, and the cutter member 3 can easily enter the bone marrow cavity. Therefore, bone marrow fluid and cancellous bone can be taken into the cutter member 3.

  In this state, the pump 8 is operated, and the connection pipe 27, the filling material container 7, the flexible tube 6 and the cutter member 3 are sucked into the negative pressure, whereby the bone marrow fluid and cancellous bone taken into the cutter member 3 are sucked. Can be introduced into the filling material container 7 through the flexible tube 6.

  In this case, prior to the suction by the pump 8, the liquid supply unit 11 is operated to supply the liquid 10 from the rear end of the cutter member 3, thereby thinning the bone marrow fluid taken into the cutter member 3 to reduce the viscosity. Can be lowered. Therefore, if the bone marrow fluid in the cutter member 3 is sucked by the operation of the pump 8 thereafter, there is an advantage that the fluidity of the bone marrow fluid can be increased and the introduction into the filling material container 7 can be facilitated. is there.

  Further, when it is desired to sufficiently suck the bone marrow fluid in the bone marrow cavity, the support member 4 is operated to change the posture of the cutter member 3 disposed in the bone marrow cavity, so that a single hole opened in the cortical bone is obtained. A large amount of bone marrow fluid can be aspirated through one hole. Therefore, a large amount of bone marrow fluid can be aspirated with minimal invasiveness, and mesenchymal stem cells contained in the bone marrow fluid can be aspirated in a healthy state.

  The bone marrow fluid or the like sucked in this way is transported into the filling material container 7 through the cutter member 3 and the flexible tube 6 as a low-viscosity fluid together with the liquid 10 supplied from the liquid supply unit 11. . Then, when entering the filling material container 7, it flows in the through hole 26 a of the biological tissue filling material 26 accommodated in the filling material container 7 and is filtered by the filter 29 arranged at the connection port to the connection pipe 27. Bone marrow cells and the like are captured, and other liquids and gases are discharged to the outside of the container body 7a.

As a result, the cells and the like captured by the filter 29 remain in the central through hole 26a of the biological tissue filling material 26, and thereby the living body in a state where the sucked cells A and the like are filled in the through hole 26a. The tissue filling body 31 can be manufactured.
And after the biological tissue filling body 31 is manufactured, as shown in FIG. 2, the fastening of the cap 7b to the container body 7a is loosened, and the cap 7b is removed to be manufactured in the filling material container 7. The biological tissue filling body 31 can be easily taken out.

  Thus, according to the biological tissue filling body manufacturing apparatus 1 according to the present embodiment, the biological tissue filling body 31 can be produced in the filling material container 7 simply by rotating the cutter member 3 and sucking it by the pump 8. it can. Therefore, the operator can perform cortical bone perforation work, bone marrow fluid suction work, suctioned cell extraction work, cell attachment work to the biological tissue filling material 26, etc. at once, and easily The filling body 31 can be manufactured. Further, at this time, since it is not necessary to replace the container with a plurality of containers, it is possible to more surely prevent the waste of cells and the occurrence of contamination.

  In the present embodiment, the cutter member 3 and the filling material container 7 are connected by the flexible tube 6, but instead, they are connected by a fixed pipe 32 as shown in FIG. May be. In this case, by configuring the fixed pipe 32 to be short, pipe friction may be reduced and the liquid supply unit 11 may be eliminated.

Moreover, you may decide to comprise the connection piping 27 of the filling material container 7 and the pump 8 with a flexible tube.
Further, as shown in FIG. 4, a recovery container 33 is disposed between the filling material container 7 and the pump 8, and the filling material container 7 and the recovery container are formed by the first connection pipe 27 a and the second connection pipe 27 b. 33, The collection container and the pump 8 may be connected.

By doing so, the liquid after the cells A are separated by the filter 29 of the filling material container 7 can be taken out without passing through the pump 8. Therefore, it is not necessary to clean the pump after use, and it can be reused.
In this case, by configuring the collection container 33 with the container body 33a and the lid body 33b, the liquid collected in the container body 33a can be easily taken out simply by removing the lid body 33b from the container body 33a. it can.

  Moreover, in the filling body manufacturing apparatus 1 according to the present embodiment, the filling material container 7 is exemplified by the flexible tube 6 and the connecting pipe 27 connected to the center of the longitudinal axis. Although the cylindrical biological tissue filling material 26 having the through hole 26a is accommodated, instead of this, as shown in FIG. 5, the axes of the flexible tube 6 and the connection pipe 27 have an eccentric axis. The filling material container 34 may be adopted.

  The filling material container 34 includes a container main body 34a and a cap 34b, and the container main body 34a and the cap 34b include rotation operation members (filling material rotating mechanisms) 35 and 36. The rotation operation members 35 and 36 are rotatably attached to the center positions of the container body 34a and the cap 34b, and face the center axis of the filling material container 34 with the cap 34b attached to the container body 34a. It is arranged.

  Each rotation operation member 35, 36 includes cylindrical knobs 35a, 36a disposed outside the container body 34a or the cap 34b, and filling material engaging portions 35b, 36b disposed inside the container body 34a or the cap 34b. And. The body tissue filling material 26 'is accommodated in the container body 34a, and the cap 34b is attached to the container body 34a, whereby the body tissue filling material is axially disposed between the filling material engaging portions 35b and 36b of the rotation operation members 35 and 36. Sandwiched between. In this state, by operating the knobs 35a and 36a of the rotation operation members 35 and 36 disposed outside the container main body 34a and the cap 34b, the biological tissue filling material 26 'is replaced with the filling material in the filling material container 34. The container 34 can be rotated around the central axis.

  In this case, as the biological tissue filling material, as shown in FIG. 5, a position eccentric from the center by the same amount as the eccentric amount of the central axis of the filling material container 34 and the central axis of the flexible tube 6 and the connecting pipe 27. A plurality of through holes 26a (for example, six places in FIG. 5B) provided at intervals in the circumferential direction are used. In the figure, reference numeral 37 denotes an engaging recess for engaging the filling material engaging portions 35b and 36b.

As a result, the rotation operation members 35 and 36 are operated to rotate the biological tissue filling material 26 ′ in the filling material container 34, and the bone marrow fluid is passed in a state where the through hole 26 a is aligned with the flexible tube 6 and the connection pipe 27. By sucking, cells can be filled into the through hole 26a. After filling the cells in one through hole 26a, the biological tissue filling material 26 'is rotated again to fill the cells in the other through holes 26a. By repeating this operation, cells can be filled in all the through holes 26a.
By doing so, it is possible to manufacture a biological tissue filling body filled with a larger amount of cells.

  Further, in the present embodiment, as shown in FIG. 6, instead of the biological tissue filling material 26 ′, the filling material container 34 is engaged with rotation operation members (rotor rotation mechanisms) 35, 36 and rotated. The plurality of cylindrical body tissue filling materials 26 may be loaded into a plurality of (four in FIG. 6B) filling material containing portions 38a provided in the rotor 38. Good. In this way, by rotating the rotor 38 and changing the biological tissue filling material 26 filled with cells, a plurality of biological tissue filling bodies can be easily manufactured.

  Moreover, in this embodiment, although the support member 4 which an operator hold | grips was provided separately from the flexible tube 6, you may provide integrally. Further, although the connection flow path 20 of the cooling device 9 is provided in the support member 4, it may be provided separately from the support member 4. Moreover, although the cooling liquid 16 supplied to the spiral flow path 19 of the cutter member 3 by the cooling device 9 is configured by physiological saline or a chemical solution, a cooling gas may be used as the cooling fluid instead.

In the present embodiment, a multi-lumen tube having a plurality of flow holes is used as the flexible tube 6, and the biological tissue filling material 26 having through holes 26a at positions corresponding to the flow holes of the multi-lumen tube is employed. May be.
Further, instead of the rotation operation units 35 and 36, a magnet (not shown) embedded in the rotor 38 may be rotated by applying a magnetic force from the outside of the filling material container 34.

Next, the biological tissue filling body manufacturing apparatus 40 according to the second embodiment of the present invention will be described with reference to FIGS. 7 and 8.
In description of this embodiment, the same code | symbol is attached | subjected to the location which shares a structure with the biological tissue filling body manufacturing apparatus 1 which concerns on 1st Embodiment mentioned above, and description is abbreviate | omitted.

  As shown in FIG. 7, the biological tissue filling body manufacturing apparatus 40 according to the present embodiment includes a substantially cylindrical filling material container portion (filling material accommodation portion) 42 that contains the biological tissue filling material 41, and the filling material. A cylindrical connecting pipe portion 43 having a small diameter extending coaxially with one end of the container portion 42, a motor (drill rotating mechanism) 44 fitted so as to close the opening at the other end of the filling material container portion 42, And a drill-like member 45 provided integrally with the rotating shaft of the motor 44.

  The drill-like member 45 passes through the inside of the filling material container part 42 and the connecting pipe part 43 and is arranged with its tip 45 a protruding from the opening of the connecting pipe part 43. A spiral groove 45 b is provided in a portion of the drill-like member 45 that is disposed in the connecting pipe portion 43 from the tip 45 a. Further, a plurality of stirring rods (stirring members) 45 c protruding in the radial direction are disposed at a portion of the drill-like member 45 disposed in the filling material container portion 42.

  In the figure, reference numeral 46 denotes a sealing member such as an O-ring for sealing a fitting portion between the filling material container portion 42 and the motor 44. Reference numeral 47 denotes a filter disposed in a through hole 48 formed through the filling material container portion 42. The filter 47 allows only passage of gas and prevents passage of liquid or the like.

The case where a biological tissue filling body is manufactured using the biological tissue filling body manufacturing apparatus 40 according to the present embodiment configured as described above will be described.
In order to manufacture a biological tissue filling body using the biological tissue filling body manufacturing apparatus 40 according to the present embodiment, first, the biological tissue filling material 41 is accommodated in the filling material container portion 42 of the living body yellow filling body manufacturing apparatus 40. To do. The biological tissue filling material 41 is preferably in the form of powder or granules. As in the first embodiment, the material is preferably β-TCP or any other biocompatible material.

  Next, the epidermis is incised to expose the surface of the long bone, and the tip 45a of the drill-like member 45 is brought into contact with the surface of the long bone. In this state, by operating the motor 44, the drill-like member 45 is rotated to perforate the long bone.

When the through-hole is formed in the long bone, the tip of the drill-like member 45 is disposed in the bone marrow cavity in the long bone, and the tip of the connecting tube portion 43 contacts the surface of the long bone, A flow path from the bone marrow cavity to the filling material container part 42 through the through hole and the connecting pipe part 43 is formed in a sealed state.
In this state, the drill-like member 45 is further rotated. Thereby, the cancellous bone and bone marrow fluid in the bone marrow cavity are pumped up through the spiral groove 45 b provided in the drill-like member 45 and sucked into the filling material container part 42. Since the through hole 48 is provided in the filling material container part 42, the gas such as air filled in the filling material container part 42 is discharged to the outside from the through hole 48 as the bone marrow fluid or the like is pumped up. The

  Since the agitating rod 45c is arranged on the drill-like member 45 in the filling material container portion 42, the aspirated bone marrow fluid and the like are mixed with the living tissue filling material 41 arranged in the filling material container portion 42 and the agitating rod 45c. And is uniformly mixed. By sucking an appropriate amount of bone marrow fluid or the like into the filling material container portion 42, a biological tissue filling body 49 in which the bone marrow fluid or the like and the biological tissue filling material 41 are uniformly mixed is manufactured.

  After the biological tissue filling body 49 is manufactured, the manufactured biological tissue is removed as shown in FIG. 8 by removing the motor 44 and the drill-like member 45 fixed to the rotating shaft from the filling material container portion 42. The filling body 49 can be easily extracted from the filling material container portion 42 together with the drill-like member 45.

It is a longitudinal cross-sectional view which shows typically the biological tissue complement manufacturing apparatus which concerns on the 1st Embodiment of this invention. It is a partial longitudinal cross-sectional view which shows the state at the time of taking out a biological tissue filling body from the filling material container of the biological tissue filling body manufacturing apparatus of FIG. It is a longitudinal cross-sectional view which shows typically the 1st modification of the biological tissue complement manufacturing apparatus of FIG. It is a longitudinal cross-sectional view which shows typically the 2nd modification of the biological tissue complement manufacturing apparatus of FIG. It is a 3rd modification of the biological tissue filling body manufacturing apparatus of FIG. 1, Comprising: (a) The longitudinal cross-sectional view of a filling material container, (b) The front view of a biological tissue filling material. It is a 4th modification of the biological tissue filling body manufacturing apparatus of FIG. 1, Comprising: (a) The longitudinal cross-sectional view of a filling material container, (b) The front view of a rotor and a biological tissue filling material. It is a longitudinal cross-sectional view which shows the biological tissue filling body manufacturing apparatus which concerns on the 2nd Embodiment of this invention. It is a longitudinal cross-sectional view which shows the state at the time of taking out a biological tissue filling body from the biological tissue filling body manufacturing apparatus of FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1,40 Biological tissue filling body manufacturing apparatus 2 Cutting blade 3 Cutter member 3a Cylindrical part (conveying pipe)
4 Support member 6 Flexible tube (conveyance tube)
7,34 Filling material container (filling material container)
7b, 34b Cap 8 Pump (suction device)
9 Cooling device 27, 27a, 27b Connection piping (conveyance tube)
29 Filter 32 Fixed piping (conveyance tube)
35, 36 Rotation operation member (filling material rotation mechanism, rotor rotation mechanism)
38 Rotor 42 Filling material container (filling material container)
43 Connection pipe (connection pipe)
44 Motor (drill rotating mechanism)
45 Drill-like member 45a Tip 45b Spiral groove 45c Stirring rod (stirring member)

Claims (9)

A delivery tube with a distal opening located in the bone marrow cavity;
A suction device for sucking the inside of the transport pipe to a negative pressure;
It is arranged at a midpoint position of the transport tube, and comprises a filling material container that houses a biological tissue filling material,
An apparatus for manufacturing a body tissue filling material, wherein the filling material container includes a filter at a suction device side end through which a liquid permeates and blocks passage of cells.   The biological tissue filling body manufacturing apparatus according to claim 1, wherein the transport tube includes a substantially cylindrical cutter member having a cutting edge at a distal end and a support member that rotatably supports the cutter member about its axis.   The biological tissue filling body manufacturing apparatus according to claim 2, wherein the cutter member is provided with a cooling device that cools the cutter member.   The filling material accommodating portion is configured in a substantially cylindrical shape that is eccentric with respect to the transport pipe, and a filling material rotating mechanism that rotates the biological tissue filling material accommodated therein around the axis of the filling material containing portion. The biological tissue complement manufacturing apparatus according to any one of claims 1 to 3, further comprising:   The filling material accommodating portion is configured in a substantially cylindrical shape that is eccentric with respect to the transfer tube, and rotatably accommodates a rotor in which a plurality of biological tissue filling materials can be loaded, and the axis of the filling material accommodating portion The biological tissue filling body manufacturing apparatus according to any one of claims 1 to 3, further comprising a rotor rotation mechanism that rotates the rotor around. A cap that is detachably attached to the distal end side of the filling material storage portion at the maximum transverse area of the filling material storage portion is provided,
The biological tissue filling body manufacturing apparatus according to any one of claims 1 to 5, wherein the transport tube is attached to the cap. A connecting tube connected to a through hole formed in the bone marrow cavity through the cortical bone;
A drill-like member having a helical groove that is rotatably inserted into the connecting tube and has a distal end disposed in the bone marrow cavity;
A drill rotation mechanism for rotating the drill-like member;
An apparatus for manufacturing a body tissue filling body, which is connected to the rear end of the connection pipe and includes a body material receiving portion for housing body tissue filling material.   The living tissue according to claim 5, wherein the drill-like member disposed in the filling material accommodation portion is provided with a stirring member that agitates the contents in the filling material accommodation portion by rotating the drill-like member. Complement body manufacturing equipment. A cap that is detachably attached to the distal end side of the filling material storage portion at the maximum transverse area of the filling material storage portion is provided,
The biological tissue filling body manufacturing apparatus according to claim 7 or 8, wherein the connection pipe is attached to the cap.

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