JP2007068636A - Cell collection apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To collect cells kept in a good state for preparing a bone graft showing a medical effect sufficiently from a patient, by a minimally invasive method. <P>SOLUTION: A cell collection apparatus 1 is provided with: a nearly cylindrical cutter member 3 having a cutting edge 2 at its tip; a support member 4 which supports the cutter member 3 rotatably around the axis; and a suction device 8 for sucking cells taken into the inside of the cutter member 3 as a result of being cut off by the cutter member 3. The cutter member 3 is provided with a cooler 9 for cooling the cutter member 3. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a cell collection device used for collecting cells such as stem cells existing in bone, for example.

Conventionally, a method of collecting cells such as bone marrow from the iliac of a patient is known (see, for example, Patent Documents 1 and 2).
According to the cell collection methods disclosed in Patent Documents 1 and 2, a large amount of bone marrow or bone tissue can be collected in a short time.
JP 2005-344299 A Japanese National Patent Publication No. 11-514905

  However, in the collection direction of Patent Document 1, there is a problem that complications may occur due to the large invasion. In addition, the collection method of Patent Document 2 has a disadvantage that a fragile cell is broken because a large load is applied to the cell during collection.

  The present invention has been made in view of the above-described circumstances, and collects cells for producing a bone graft that sufficiently exhibits a medical effect from a patient with a minimally invasive method in a good state. An object of the present invention is to provide a cell collection device capable of performing the above.

In order to achieve the above object, the present invention provides the following means.
The present invention provides a substantially cylindrical cutter member having a cutting edge at the tip, a support member that supports the cutter member so as to be rotatable about its axis, and a result of being cut by the cutter member. And a suction device for sucking the taken-in cells, and a cell collection device provided with a cooling device for cooling the cutter member.

  According to the present invention, the cutting edge of the substantially cylindrical cutter member is brought into contact with the outer surface of the cortical bone of the long bone, for example, the support member is supported, and the cutter member is supported. By rotating with respect to the member, the cortical bone is cut and the tip of the cutter member is inserted into the bone space where the cancellous bone and bone marrow fluid in the cortical bone are arranged. By further advancing the cutting edge at the tip of the cutter member in the bone marrow cavity, cancellous bone and bone marrow fluid containing cells are taken up inside the cutter member. Then, by the operation of the suction device, the taken-in cells are sucked and collected.

  In this case, the bone marrow fluid containing the cells inside can be collected and the cells can be collected in a minimally invasive manner simply by piercing the cortical bone with the cutting edge provided at the tip of the substantially cylindrical cutter member. Therefore, the burden on the patient can be reduced.

  In addition, friction between the cutter member and cortical bone is generated by rotation of the cutter member, and frictional heat is generated. However, since the cutter member is equipped with a cooling device, the temperature of the cutter member increases due to the operation of the cooling device. Is suppressed. Therefore, the cells contained in the bone marrow fluid taken inside the cutter member are not damaged by heat and are recovered in a healthy state.

  Further, the present invention provides a substantially cylindrical cutter member having a cutting edge at the tip, a support member that rotatably supports the cutter member around its axis, and a result of being cut by the cutter member. And a holding means for holding the cells taken in the inside of the cutter member. The cell collecting device includes a cooling device for cooling the cutter member.

  According to the present invention, a cutting edge of a substantially cylindrical cutter member is brought into contact with a part from which cells are to be collected, for example, a cortical bone outer surface of a long bone, and the cutter member is rotated with respect to a support member. Thus, the cortical bone is cut, and the tip of the cutter member is inserted into the bone space where the cancellous bone and bone marrow fluid in the cortical bone are arranged. By further advancing the cutting edge at the tip of the cutter member in the bone marrow cavity, bone marrow fluid containing cells is taken inside the cutter member. And a cell is collect | recovered by hold | maintaining the taken-in cell in a cutter member by the action | operation of a holding means.

  In this case, the cancellous bone and bone marrow fluid containing the internal cells can be collected and the cells can be collected with minimal invasiveness by simply drilling into the cortical bone with the cutting edge provided at the tip of the substantially cylindrical cutter member. . Therefore, the burden on the patient can be reduced.

  In addition, friction between the cutter member and cortical bone is generated by rotation of the cutter member, and frictional heat is generated. However, since the cutter member is equipped with a cooling device, the temperature of the cutter member increases due to the operation of the cooling device. Is suppressed. Therefore, the cells contained in the bone marrow fluid taken inside the cutter member are not damaged by heat and are recovered in a healthy state.

In the said invention, the said cooling device is good also as distribute | circulating a cooling fluid to the flow path formed inside the cutter member.
By doing in this way, the whole cutter member can be cooled simply and efficiently, and the soundness of a cell can be maintained.

Moreover, in the said invention, it is good also as providing the liquid supply part which supplies a liquid inside the said cutter member.
By doing this, the viscosity of the bone marrow fluid containing the cells taken into the inside of the cutter member is lowered, the generated friction is reduced, the ease of suction by the suction device is improved, and the cells are healthy and efficient Can be recovered. In addition, a further cooling effect can be achieved by adjusting the temperature of the liquid to be supplied.

Moreover, in the said invention, it is preferable that the flexible tube which has a cavity inside at the rear end of the said cutter member, and has flexibility is connected.
By doing so, it is possible to change the posture of the cutter member by bending the flexible tube, and to recover many cancellous bone and bone marrow fluid in the bone marrow cavity from a single hole made in the cortical bone. .

Moreover, in the said invention, it is good also as providing the rotating apparatus which rotates the said cutter member around the axis line.
By rotating the cutter member with the rotating device, the cortical bone can be more easily perforated and the cells in the bone marrow cavity can be collected.

Further, in the above invention, the flexible tube may be configured to transmit a rotational force, and may include a rotating device that applies a rotational force to the proximal end side of the flexible tube and rotates the cutter member around its axis. Good.
By doing so, the rotating device is arranged on the proximal end side of the flexible tube, the size and weight of the cutter member arranged on the distal end side of the flexible tube is reduced, and the flexible tube is bent to perform cell collection work. The handling property of the cutter member at the time of performing can be improved.

Moreover, in the said invention, the said holding means is good also as being comprised by the protrusion or groove | channel formed in the inner surface of a cutter member.
By doing this, the projections or grooves formed on the inner surface of the cutter member hook and lock the bone marrow fluid or cancellous bone taken into the inner surface of the cutter member, and hold the cells in the cutter member with a simple configuration can do.

Moreover, in the said invention, the said holding means is good also as being formed in the spiral shape which advances by rotation at the time of the cutting | disconnection by a cutter member.
In this way, when the cutter member is rotated, the cancellous bone and bone marrow fluid can be smoothly taken into the cutter member by taking the cancellous bone and bone marrow fluid along the spiral protrusion or groove. When the cutter member is taken out from the bone marrow cavity, the bone marrow fluid or cancellous bone can be hooked into the protrusion or groove and held so as not to be discharged from the cutter member simply by pulling the cutter member in the axial direction.

Moreover, in the said invention, the said holding means is good also as being comprised by the movable protrusion provided so that it could advance / retreat to radial inside from the inner surface of a cutter member.
With this configuration, when the cutter member is inserted into the bone marrow cavity, the movable protrusion is retracted radially outward so that the bone marrow fluid is smoothly taken into the cutter member, and the cutter member is removed from the bone marrow. When taking out from the cavity, the movable protrusion can be protruded inward in the radial direction, and bone marrow fluid or cancellous bone can be hooked and held and taken out from the cutter member.

Moreover, in the said invention, the said holding means is good also as providing the cutting device which cut | disconnects the cancellous bone taken in inside the said cutter member in the front end side of a cutter member.
By doing in this way, after taking bone marrow fluid into the cutter member, by operating the cutting device and cutting the cancellous bone taken together with the bone marrow fluid at the tip side of the cutter member, it was taken into the cutter member When the cancellous bone is separated from the cancellous bone in the bone marrow cavity and the cutter member is taken out from the bone marrow cavity, the bone marrow fluid in the cutter member is prevented from being discharged to the outside by being dragged by the cancellous bone in the bone marrow cavity. The bone marrow fluid can be retained in the cutter member.

  According to the present invention, there is an effect that cells for producing a bone graft that sufficiently exerts a medical effect from a patient can be collected in a good state by a minimally invasive method.

A cell collection device 1 according to an embodiment of the present invention will be described below with reference to FIG.
As shown in FIG. 1, the cell collection device 1 according to the present embodiment is formed in a substantially cylindrical shape, has a cutter member 3 having a cutting edge 2 at the tip, and a support member that rotatably supports the cutter member 3. 4, a rotating device 5 that applies a rotational force to the cutter member 3, a flexible tube 6 having one end connected to the rear end of the cutter member 3, a collection container 7 disposed at the other end of the flexible tube 6, The recovery container 7, a flexible tube 6 connected to the recovery container 7, and a pump 8 that decompresses the inside of the cutter member 3 are provided. The cutter member 3 includes a cooling device 9, and the flexible tube 6 is connected to a liquid supply unit 11 that supplies the liquid 10 into the cutter member 3.

  The cutter member 3 includes a tubular portion 3a formed in a substantially cylindrical tubular shape, and a plurality of cutting blades 2 arranged at the distal end of the tubular portion 3a and arranged in the circumferential direction with the sharp pointed ends in the distal direction. ing. Thus, the cutter blade 3 is rotated around its central axis while the tip of the cutting blade 2 is in contact with an object to be drilled (not shown) (for example, the outer surface of the cortical bone of the long bone). The object to be drilled is shaved and the object to be drilled can be drilled.

  The support member 4 is a substantially cylindrical member that is disposed at a midway position in the longitudinal direction of the cutter member 3 so as to surround the cutter member 3, and supports the cutter member 3 rotatably by a bearing 12. is doing. The support member 4 can be operated by an operator by hand.

  The rotating device 5 includes a motor 13 fixed to the support member 4 and gears 14 and 15 fixed to the motor 13 and the cutter member 3 and meshing with each other. By holding the support member 4 and operating the motor 13, the gears 14 and 15 can be rotated to rotate the cutter member 3 around the central axis.

  The cooling device 9 is connected to a container 17 for storing a coolant 16, for example, physiological saline or other chemicals, and a pipe 18 that connects the container 17 and the support member 4 and supplies the coolant 16 to the support member 4. A spiral flow path 19 formed inside the cutter member 3 and a connection flow path 20 connecting the pipe 18 and the flow path 19.

  The connection channel 20 includes a ring-shaped space 22 defined in a sealed state by two seal members 21 that are spaced apart in the axial direction between the cutter member 3 and the support member 4, and the cutter member 3. And a circumferential groove 23 formed on the outer surface and communicating with the ring-shaped space 22. The flow path 19 is connected to the circumferential groove 23.

  Thereby, even in a state where the cutter member 3 is rotated with respect to the support member 4, the coolant 16 is supplied from the container 17 into the flow path 19 through the pipe 18, the ring-shaped space 22, and the circumferential groove 23. The cutter member 3 provided with the flow path 19 can be cooled as a whole. The cooling liquid 16 is supplied from the container 17 by, for example, a pump (not shown).

  In addition, the liquid supply unit 11 includes, for example, a container 24 that stores a liquid 10 such as physiological saline or other chemical solution, and a pipe 25 that connects the container 24 and the flexible tube 6. The liquid 10 stored in the container 24 is supplied into the flexible tube 6 by, for example, gravity or by a pump (not shown), and is supplied into the cutter member 3 connected to the flexible tube 6. It has become.

The collection container 7 includes a container body 7b that can be opened and closed in a sealed state by a lid 7a. A pipe 26 connected to the flexible tube 6 and the pump 8 is attached to the lid 7a. By removing the lid 7a from the container body 7b, the cells and the like collected in the container body 7a can be easily taken out.
In the figure, reference numeral 27 denotes a seal member that seals between the cutter member 3 and the flexible tube 6, and reference numeral 28 denotes a filter.

The operation of the cell collection device 1 according to the present embodiment configured as described above will be described below.
In order to collect cells, for example, mesenchymal stem cells in the long bone, using the cell collection device 1 according to this embodiment, the surface of the long bone is exposed by incising the epidermis, and the support member 4 is operated. Then, the cutting blade 2 at the tip of the cutter member 3 is brought into contact with the long bone surface. At this time, since the proximal end side of the cutter member 3 is connected to the flexible tube 6, the posture of the cutter member 3 can be freely adjusted by bending the flexible tube 6, and the hole to be drilled in the long bone can be obtained. The cutter member 3 can be aligned with the angle and direction. In this state, the cooling device 9 and the rotating device 5 are operated, and the cutter member 3 is rotated around its central axis in a state where the coolant 16 is circulated in the spiral flow path 19 of the cutter member 3.

  By applying a pressing force toward the distal end of the cutter member 3 to the support member 4, the cutting blade 2 at the distal end of the cutter member 3 is pressed against the long bone, and the cortical bone of the long tubular bone is cut by the rotation of the cutting blade 2. Can be perforated. In this case, when the cutting blade 2 is rotated with respect to the cortical bone, frictional heat is generated due to friction between the cortical bone and the cutting blade 2, but the coolant 16 is supplied to the flow path 19 inside the cutter member 3. Therefore, it is avoided that the cutter member 3 is in a high temperature state.

  When the cutting blade 2 penetrates the cortical bone and is inserted into the bone marrow cavity, the bone marrow fluid contained in the bone marrow cavity flows into the cutter member 3. In this case, since the cutter member 3 is cooled so as not to reach a high temperature state, the bone marrow fluid flowing into the cutter member 3 is prevented from being heated, and the soundness of the mesenchymal stem cells in the bone marrow fluid is prevented. Can be prevented from being damaged by heat. The cancellous bone is also present in the bone marrow cavity, but the cancellous bone is also cut when the cutting blade 2 at the tip of the cutter member 3 comes into contact, and the cutter member 3 can easily enter the bone marrow cavity. Therefore, bone marrow fluid and cancellous bone can be taken into the cutter member 3.

  In this state, the pump 8 is operated, and the pipe 26, the collection container 7, the flexible tube 6 and the cutter member 3 are sucked to a negative pressure, so that the bone marrow fluid and cancellous bone taken into the cutter member 3 are flexible. It can be recovered in the recovery container 7 via the tube 6.

  In this case, prior to the suction by the pump 8, the liquid supply unit 11 is operated to supply the liquid 10 from the rear end of the cutter member 3, thereby thinning the bone marrow fluid taken into the cutter member 3 to reduce the viscosity. Can be lowered. Therefore, if the bone marrow fluid in the cutter member 3 is sucked by the operation of the pump 8 after that, there is an advantage that the fluidity of the bone marrow fluid can be increased and the recovery to the recovery container 7 can be facilitated. .

  Further, when it is desired to sufficiently suck the bone marrow fluid in the bone marrow cavity, the support member 4 is operated to change the posture of the cutter member 3 disposed in the bone marrow cavity, so that a single hole opened in the cortical bone is obtained. Many bone marrow fluids can be aspirated and collected through one hole. Therefore, a large amount of bone marrow fluid can be collected with minimal invasiveness, and mesenchymal stem cells contained in the bone marrow fluid can be collected in a healthy state.

  In the present embodiment, the support member 4 held by the operator is provided separately from the flexible tube 6, but may be provided integrally. Further, although the connection flow path 20 of the cooling device 9 is provided in the support member 4, it may be provided separately from the support member 4. Moreover, although the cooling liquid 16 supplied to the spiral flow path 19 of the cutter member 3 by the cooling device 9 is configured by physiological saline or a chemical solution, a cooling gas may be used as the cooling fluid instead.

Next, a cell collection device 30 according to a second embodiment of the present invention will be described with reference to FIG.
In the description of the present embodiment, the same reference numerals are given to portions having the same configuration as the cell collection device 1 according to the first embodiment described above, and the description thereof is omitted.

  As shown in FIG. 2, the cell collection device 30 according to the present embodiment includes a flexible shaft 31 on the proximal end side of the cutter member 3, a point including a fixed pipe 32 instead of the flexible tube 6, and a rotating device. It differs from the cell collection device 1 according to the first embodiment in that 33 is attached to the fixed pipe 32 instead of the support member 4.

The flexible shaft 31 is fixed to the proximal end side of the cutter member 3, can be curved relatively freely, and can transmit a rotational force around its central axis.
The rotating device 33 includes a motor 34 fixed to a fixed pipe 32 and gears 35 and 36 which are fixed to the motor 34 and the flexible shaft 31 and mesh with each other.

  According to the cell collection device 30 according to the present embodiment configured as described above, the operator holds the support member 4 and operates it, thereby bending the flexible shaft 31 and handling the cutter member 3 to obtain a desired posture. And the cutter device 3 is rotated while being cooled by operating the cooling device 9 and the rotating device 33. In this case, the rotational force of the motor 34 is transmitted to the flexible shaft 31 via the gears 35 and 36, and is transmitted in the longitudinal direction by the flexible shaft 31 to rotate the cutter member 3.

  Thus, according to the cell collection device 30 according to the present embodiment, the rotating device 33 that rotates the cutter member 3 is attached to the fixed pipe 32 instead of the support member 4 handled by the operator. There is an advantage that the periphery of the member 3 and the support member 4 can be reduced in size and weight, and the handleability can be improved.

Next, a cell collection device 40 according to a third embodiment of the present invention will be described with reference to FIGS.
In the description of the present embodiment, the same reference numerals are given to portions having the same configuration as the cell collection device 1 according to the first embodiment described above, and the description thereof is omitted.

The cell collection device 40 according to the present embodiment is of the type in which the cell collection devices 1 and 30 according to the first and second embodiments are sucked by a pump, whereas the cells are collected inside the cutter member 41. It is a method of holding and taking out.
In the present embodiment, the cutter member 41 includes a spiral protrusion (holding means) 42 on its inner surface. The protrusion 42 extends so that the tip inward in the radial direction is inclined from the tip of the cutter member 41 toward the rear end side, and the bone marrow fluid A taken from the tip of the cutter member 41 is easily taken in, The bone marrow fluid A that is about to flow out to the distal end side is held so as not to be caught by the protrusion 42 and flow out.

  In the figure, reference numeral 43 denotes a handle member fixed to the support member 4. A through hole 44 is formed in the center of the handle member 43, and the through hole 44 communicates with the inside of the cutter member 41.

The operation of the cell collection device 40 according to this embodiment configured as described above will be described.
For example, in order to collect cancellous bone and bone marrow fluid A in the long bone using the cell collection device 40 according to this embodiment, the operator operates the handle member 43 to cut the tip of the cutter member 41. The cutter member 41 is rotated around the central axis by pressing the blade 2 against the cortical bone surface of the long bone and operating the rotating device 5. At this time, operating the cooling device 9 is the same as described above. Thereby, a through-hole can be formed in the cortical bone without heating the cutter member 41 by frictional heat.

From this state, by further rotating the cutter member 41 and inserting the cutter member 41 into the bone marrow cavity, the bone marrow fluid A in the bone marrow cavity is taken into the inside of the cutter member 41.
In this case, the rotation direction of the cutter member 41 is determined by the twisting direction of the spiral protrusion 42 formed on the inner surface. For example, in the example shown in FIG. 3, since the spiral protrusion 42 is formed like a right-hand thread, the cutter member 41 is rotated clockwise in the traveling direction.

  As a result, the bone marrow fluid A in the bone marrow cavity is taken into the cutter member 41 along the spiral projection 42 inside by rotation of the cutter member 41. In this state, by extracting the cutter member 41 from the bone marrow cavity, the bone marrow fluid A taken into the cutter member 41 can be taken out from the body. Since the bone marrow fluid A has sufficient viscosity, as shown in FIG. 4, the bone marrow fluid A is caught by the tip of the protrusion 42 extending from the tip to the back side and is held in the cutter member 41. The Thereby, bone marrow fluid A containing mesenchymal stem cells can be collected in a healthy state. Here, the bone marrow fluid A also includes cancellous bone, as in the first embodiment.

  In this case, since the bone marrow fluid A held in the cutter member 41 is difficult to take out from the cutter member 41 as it is, as shown in FIG. The pressing member 45 may be inserted and pushed out from the rear end side. At that time, if the cutter member 41 is rotated counterclockwise, the bone marrow fluid A is smoothly pushed out along the spiral protrusion 42. The bone marrow fluid A in the cutter member 41 may be poured out and collected by pouring physiological saline through the through hole 44 of the handle member 43.

  In the present embodiment, as the holding means, the spiral protrusion 42 is provided on the inner surface of the cutter member 41. However, instead of this, a spiral groove (not shown) may be used. Further, the projection is not limited to a spiral shape, and may be any other form of protrusion or groove.

Alternatively, as shown in FIG. 6, a movable protrusion 42 ′ may be provided in the cutter member 41 ′.
In the example shown in FIG. 6, the movable protrusion 42 ′ is formed in a leaf spring member 47 accommodated in a groove 46 provided along the axial direction inside the cutter member 41 ′. The leaf spring member 47 is configured to move the distal end side in the radial direction by operating the operating means 48 on the proximal end side of the cutter member 41 ′.

  That is, one end of the leaf spring member 47 is fixed on the proximal end side of the cutter member 41 ′. Reference numeral 47a denotes a mounting member such as a bolt. In addition, a plurality of movable protrusions 42 ′ extending in the radial direction are arranged on the front end side of the leaf spring member 47 at intervals in the longitudinal direction. The operation means 48 is, for example, an operation sleeve 49 that is fitted to the outer surface of the cutter member 41 ′ so as to be movable along the longitudinal direction, and a pressure that is pressed radially inward by a tapered inner surface 49 a of the operation sleeve 49. Rod 50.

  The pressing rod 50 is movably inserted into a through hole 51 that penetrates the cutter member 41 ′ in the radial direction. A plurality of through holes 51 are provided at intervals in the circumferential direction, and are sealed by a seal member 52 such as an O-ring disposed around the pressing rod 50.

  The pressing rod 50 has a length dimension capable of protruding inward and outward in the radial direction of the through hole 51. When the pressing rod 50 is pressed inward in the radial direction by the tapered inner surface 49a of the operation sleeve 49, the pressing rod 50 protrudes toward the inner surface side of the cutter member 41 ′. As a result of pressing the leaf spring member 47 in the radial direction, the free end of the leaf spring member 47 can be displaced inward in the radial direction. Further, when the operating sleeve 49 is moved in the reverse direction to release the pressing force against the pressing rod 50, the pressing rod 50 protrudes radially outward by the elastic force of the leaf spring member 47, and the leaf spring member 47 becomes the groove 46. It is designed to be restored to the position accommodated inside.

  In this way, when the bone marrow fluid A is taken into the cutter member 41 ′, the movable protrusion 42 ′ is accommodated in the groove 46 as shown in FIG. Inflow can be enabled. Further, when the bone marrow fluid A taken in the cutter member 41 ′ is held, as shown in FIG. 5B, the operation sleeve 49 is slid to project the pressing rod 50 inward in the radial direction, and the leaf spring member 47. By elastically deforming and projecting the movable protrusion 42 ′ radially inward from the groove 46, the bone marrow fluid A is caught on the movable protrusion 42 ′ and held so as not to be discharged from the tip of the cutter member 41 ′. it can.

  In addition, the cell collection device 40 according to the present embodiment can be used not only for collecting bone marrow fluid A from long bones but also for uses as shown in FIGS. That is, when there is a site C that needs to be repaired at a midway position in the longitudinal direction of the long bone B, first, as shown in FIG. 7, healthy mesenchymal stem cells in the long bone B The bone marrow fluid is retained by inserting the cutter member 41 into the site D where the sac is present. Next, the cutter member 41 is advanced as it is in the bone marrow cavity E, so that the distal end holding the bone marrow fluid A reaches the site C. By releasing the retained bone marrow fluid A at this position, it is possible to perform a minimally invasive treatment without performing two operations.

According to the cell collection device 40 according to the present embodiment, since the cells are held in a healthy state, the mesenchymal stem cells released to the repair site C grow healthy and repair the repair site C quickly. It becomes possible to do. In addition, as shown by an arrow F, a granular calcium phosphate porous material serving as a scaffold may be introduced or a growth factor may be introduced through a through hole 44 provided in the handle member 43.
A scale (not shown) for monitoring the insertion depth of the cutter member 41 outside the body may be attached in parallel to the cutter member 41 itself or the cutter member 41.

Next, a cell collection device 60 according to a fourth embodiment of the present invention will be described with reference to FIGS.
The cell collection device 60 according to the present embodiment includes a cutting means 61 for cutting the cancellous bone taken into the cutter member 41 ″ at the tip of the cutter member 41 ″.

  In the bone marrow cavity, cancellous bone is contained in addition to the bone marrow fluid. If this cancellous bone is not cut, the bone marrow fluid once taken into the cutter member 41 ″ will be transferred to the cancellous bone remaining in the bone marrow cavity. It is conceivable that the blade is dragged and discharged from the cutter member 41 ″.

  Therefore, for example, in the example shown in FIG. 9, on the inner surface of the cutter member 41 ″, a strip plate member 62 disposed along the longitudinal direction, an operation piece 63 operated on the proximal end side of the cutter member 41 ″, A connecting shaft 65 that is disposed in a long hole 64 that penetrates the cutter member 41 ″ in the radial direction and connects the operation piece 63 and the strip plate member 62, and a guide surface 66 that is provided on the inner surface of the cutter member 41 ″. Thus, the cutting means 61 is configured.

  The front end portion 62a of the band plate member 62 is formed to have a small width so as to be easily bent by elastic deformation, and the peripheral edge of the front end portion 62a is formed sharply so that the cancellous bone can be cut. As shown in FIG. 10, the band plate member 62 has a width dimension sufficiently larger than the width dimension of the long hole 64, and closes the long hole 64 in a sealed state. Further, since the strip plate member 62 is in close contact with the inner surface of the cutter member 41 ″ with a certain width, the strip plate member 62 is slightly curved in the width direction, and the linearity along the longitudinal direction is maintained. It has become so.

  Further, the connecting shaft 65 has a dimension capable of moving in the longitudinal direction of the cutter member 41 ″ in the long hole 64. Further, the operation piece 63 is the amount of movement of the connecting shaft 65 in the long hole 64. The cutter member 41 ″ is provided so as to be slidable in the longitudinal direction, and has a size capable of covering the entire long hole 64 at any sliding position. Thereby, the long hole 64 is closed by the strip plate member 62 and the operation piece 63 inside and outside in the radial direction, and the inside and outside of the cutter member 41 ″ are sealed.

  The guide surface 66 is formed in a cylindrical inner surface that smoothly continues radially inward from the inner surface of the cutter member 41 ″. The tip end portion 62a of the strip plate member 62 is moved along the guide surface 66. Thus, the band plate member 62 can be bent by approximately 90 °, and the tip end thereof can be protruded in the radial direction.

  In order to collect cells using the cell collection device 60 according to the present embodiment configured as described above, as shown in FIG. 9A, FIG. 10A, and FIG. By sliding 63 to the rear end side, the strip plate member 62 is slid to the rear end side, and the front end portion 62a of the strip plate member 62 is disposed radially outward. In this state, the cutter member 41 ″ is rotated to make a through-hole in the cortical bone and advance while cutting the cancellous bone in the bone marrow cavity, and the tip of the cutter member 41 ″ is inserted to some extent deeply in the bone marrow cavity. By the way, the rotation of the cutter member 41 ″ is stopped.

  Thereby, since the bone marrow fluid and cancellous bone are taken into the cutter member 41 ″, the operation piece 63 is inserted into the cutter member 41 as shown in FIG. 9 (b), FIG. 10 (b) and FIG. 11 (b). The tip end portion 62a of the band plate member 62 is protruded inward in the radial direction by being slid toward the tip end side. In this state, as shown by an arrow G in FIG. 11 (c), the cutter member 41 ″ is rotated one or more times. Thereby, the tip 62a of the band plate member 62 causes the sponge in the cutter member 41 ″. The bone is cut.

  Therefore, even if the cutter member 41 ″ is subsequently pulled out in the longitudinal direction, the bone marrow fluid and cancellous bone inside are prevented from being pulled out of the cutter member 41 ″. That is, since bone marrow fluid containing cancellous bone is held in a state where it is accommodated in the cutter member 41 ″, cells in a healthy state can be collected by removing the cutter member 41 ″ from the body.

1 is a longitudinal sectional view schematically showing a cell collection device according to a first embodiment of the present invention. It is a longitudinal cross-sectional view which shows typically the cell collection device which concerns on the 2nd Embodiment of this invention. It is a longitudinal cross-sectional view which shows typically the cell collection device which concerns on the 3rd Embodiment of this invention. FIG. 4 is a partial enlarged cross-sectional view showing a state in which bone marrow fluid is held by the cell collection device of FIG. 3. It is a figure explaining the case where the bone marrow fluid hold | maintained with the cell collection device in FIG. 4 is taken out. It is a longitudinal cross-sectional view which shows the modification of the cell collection device of FIG. It is explanatory drawing which shows typically the usage example of the cell collection device of FIG. It is explanatory drawing similar to FIG. It is a longitudinal cross-sectional view which shows partially the cell collection device which concerns on the 4th Embodiment of this invention. FIG. 10 is a side view partially showing the cell collection device of FIG. 9. It is the front view which looked at the cell collection device of Drawing 9 from the tip side.

Explanation of symbols

1, 30, 40, 60 Cell collection device 2 Cutting blade 3, 41, 41 ′, 41 ″ Cutter member 4 Support member 5 Rotating device 6 Flexible tube 8 Pump (suction device)
9 Cooling device 10 Liquid 11 Liquid supply unit 16 Coolant (cooling fluid)
19 Channel 31 Flexible shaft (flexible tube)
42 Protrusion (holding means)
42 'movable protrusion (holding means)
61 Cutting means (holding means)

Claims (11)

A substantially cylindrical cutter member having a cutting edge at the tip;
A support member that rotatably supports the cutter member about its axis;
As a result of being cut by the cutter member, a suction device for sucking cells taken inside the cutter member,
A cell collection device comprising a cooling device for cooling the cutter member on the cutter member. A substantially cylindrical cutter member having a cutting edge at the tip;
A support member that rotatably supports the cutter member about its axis;
As a result of being cut by the cutter member, and holding means for holding the cells taken inside the cutter member in the cutter member,
A cell collection device comprising a cooling device for cooling the cutter member on the cutter member.   The cell collection device according to claim 1 or 2, wherein the cooling device causes a cooling fluid to flow through a flow path formed inside the cutter member.   The cell collection device according to claim 1, further comprising a liquid supply unit configured to supply a liquid to the inside of the cutter member.   The cell collection device according to any one of claims 1 to 4, wherein a flexible tube having a cavity is connected to a rear end of the cutter member.   The cell collection device according to any one of claims 1 to 5, further comprising a rotation device that rotates the cutter member about its axis. The flexible tube is configured to transmit a rotational force,
The cell collection device according to claim 5, further comprising a rotating device that applies a rotational force to the proximal end side of the flexible tube and rotates the cutter member around its axis.   The cell collection device according to claim 2, wherein the holding means is constituted by a protrusion or a groove formed on the inner surface of the cutter member.   The cell collection device according to claim 8, wherein the protrusion or groove is formed in a spiral shape that advances by rotation during cutting by a cutter member.   The cell collection device according to claim 2, wherein the holding means is configured by a movable protrusion provided so as to be able to advance and retract in the radial direction from the inner surface of the cutter member.   The cell collection device according to claim 2, wherein the holding unit includes a cutting device that cuts cancellous bone taken into the cutter member at a distal end side of the cutter member.

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