JP2007091734A - Modified water, oxidative stress-resisting agent containing the same, cell growth promoting agent, and vitality enhancing agent - Google Patents
Modified water, oxidative stress-resisting agent containing the same, cell growth promoting agent, and vitality enhancing agent Download PDFInfo
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Abstract
Description
本発明は、原料水に外的刺激を加え原料水の構造のみを制御することによって改質した抗酸化作用を有する改質水及びそれを含有する抗酸化ストレス剤、細胞増殖促進剤並びに活力増強剤に関する。 The present invention relates to a modified water having an anti-oxidation effect which is modified by applying an external stimulus to the raw material water and controlling only the structure of the raw material water, an antioxidant stress agent containing the same, a cell growth promoter and a vitality enhancement. It relates to the agent.
近年、活性酸素が、老化，ガン，糖尿病，動脈硬化症，肝炎，腎炎，アトピー性皮膚炎など様々な疾病の原因になっていることが明らかになってきつつある。また、大気，土壌及び水の汚染が活性酸素の発生率を高め、健康障害を起こしていると懸念されている。
活性酸素は、体内に吸い込んだ酸素を利用してエネルギーを生み出す過程で生成すると言われており、生体防御では重要な働きをしている。一方で過剰な活性酸素は、老化，動脈硬化，脳卒中，心筋梗塞，ガンといった疾病の原因になると言われており、生体に有害な作用を及ぼすことが最近の研究で明らかにされつつある。このように、酸化ストレスは様々な疾病の進展、発症、増悪に関与していることが明らかになってきている。
このような酸化ストレスを抑制するものとして、赤ワインやお茶に含まれるポリフェノール、あるいはビタミンＣなどが知られている。これらは抗酸化作用を有しているため、老化予防，動脈硬化の予防，免疫力を高める，肝臓機能低下の予防といった作用があり、医療、保健、健康などの分野に応用されている。
In recent years, it has become clear that active oxygen is responsible for various diseases such as aging, cancer, diabetes, arteriosclerosis, hepatitis, nephritis, and atopic dermatitis. There is also concern that air, soil and water contamination will increase the rate of active oxygen generation and cause health problems.
Active oxygen is said to be generated in the process of generating energy using oxygen inhaled into the body, and plays an important role in biological defense. On the other hand, excessive active oxygen is said to cause diseases such as aging, arteriosclerosis, stroke, myocardial infarction, and cancer, and recent studies have revealed that it has harmful effects on living bodies. Thus, it has become clear that oxidative stress is involved in the development, onset and exacerbation of various diseases.
As a material for suppressing such oxidative stress, polyphenols or vitamin C contained in red wine or tea are known. Since these have an antioxidant action, they have effects such as prevention of aging, prevention of arteriosclerosis, enhancement of immunity, and prevention of liver function decline, and they are applied in fields such as medical treatment, health care and health.
また、このような酸化ストレスを抑制するものとして、抗酸化機能を付与した飲料水の開発が行われている。水は生体膜や脳血液関門も透過する唯一の物質であり、しかも、人体の約７０％を占めており人間の生命活動の源であるといっても過言ではないため、抗酸化能を付与できれば、効率よく人体に作用させることができ、かつ人体には負荷が少なく、ひいては地球環境に負荷をかけることがないからである。
従来、水に抗酸化機能を付与する技術として、例えば以下の様なものが知られている。
（特許文献１）には、「原水に水素をバブリングや加圧充填して溶存させて水素溶存水を製造する過程で、又は、水の電気分解によって水素溶存水を製造する過程で、ＰｔやＰｄコロイドなどの触媒を作用させる水処理方法及び抗酸化水」が開示されている。
（特許文献２）には、「常温常圧下でｐＨが６．５〜９であり、酸化還元電位が−９００〜−１５０ｍＶである中性還元水からなる抗酸化性水」が開示されている。
（特許文献３）には、「二酸化炭素等を溶解させた酸性水にドロマイト等を溶解し、これを電解法より得られたアルカリ還元水で希釈することにより抗酸化性の機能を有するミネラル水を調製する製造法」が開示されている。
Conventionally, for example, the following techniques are known as techniques for imparting an antioxidant function to water.
(Patent Document 1) states that “in the process of producing hydrogen-dissolved water by bubbling or pressurizing and filling hydrogen into raw water to produce hydrogen-dissolved water, or in the process of producing hydrogen-dissolved water by electrolysis of water, A water treatment method and antioxidant water using a catalyst such as Pd colloid is disclosed.
(Patent Document 2) discloses “antioxidant water composed of neutral reduced water having a pH of 6.5 to 9 and an oxidation-reduction potential of −900 to −150 mV under normal temperature and normal pressure”. .
(Patent Document 3) states that “mineral water having an antioxidative function by dissolving dolomite or the like in acidic water in which carbon dioxide or the like is dissolved, and diluting this with alkaline reduced water obtained by an electrolytic method. Is disclosed.
しかしながら上記従来の技術においては、以下のような課題を有していた。
（１）特許文献１乃至３に開示の技術は、原水を電気分解したり原水に水素ガスを溶解したりすることによって水に抗酸化機能を付与するものであるため、水の電気分解等による改質処理のために電気エネルギー等を要し、省エネルギー性に欠けるという課題を有していた。
（２）水の電解処理によって製造された還元水がアルカリ性を有していたり、低い酸化還元電位を有していると、個人差はあるが、飲用した場合に血中のカリウム濃度を上昇させたり便秘や下痢症状を呈したりすることがあり、人体に負担をかけることがあるという課題を有していた。
However, the above conventional techniques have the following problems.
(1) The techniques disclosed in
(2) If the reduced water produced by the electrolytic treatment of water has alkalinity or has a low redox potential, there will be individual differences, but if you drink it, it will increase the potassium concentration in the blood. It may cause constipation and diarrhea, and may cause a burden on the human body.
本発明は、上記従来の課題を解決するもので、水の構造を変化させて抗酸化性を付与し、改質水を摂取した人体が負担を受けることなく、線維芽細胞等の動物由来の細胞に対する酸化ストレスの抑制、線維芽細胞等の動物由来の細胞に対する増殖促進作用、植物や魚介類等の鮮度の保持や活力増強、動物の整腸効果といった特異的な効果が得られ、また水の電気分解等による改質処理のような多大なエネルギーを必要とせず、省エネルギー性に優れた改質水を提供することを目的とする。
また本発明は、線維芽細胞などの動物由来の細胞に対し非常に強い抗酸化作用を有し、酸化ストレスが関与する疾患の症例に対して抑制的に作用し優れた抗酸化ストレス作用を有する抗酸化ストレス剤を提供することを目的とする。
また本発明は、線維芽細胞等の動物由来の細胞に対する優れた増殖促進作用を有し、創傷治癒の促進等に優れた細胞増殖促進剤を提供することを目的とする。
また本発明は、植物や魚介類等に対して高い鮮度保持性を有し、淡水・海水魚等の魚介類の飼育、養殖などに使用することによって魚介類の生理活性及び免疫力増強をも期待できる活力増強剤を提供することを目的とする。
The present invention solves the above-mentioned conventional problems, imparts antioxidant properties by changing the structure of water, and is derived from animals such as fibroblasts without burdening the human body ingesting the modified water. Specific effects such as suppression of oxidative stress to cells, promotion of growth of cells derived from animals such as fibroblasts, maintenance of freshness and vitality of plants and seafood, and animal intestinal effects are obtained. An object of the present invention is to provide reformed water that does not require a large amount of energy as in the reforming process by electrolysis or the like and is excellent in energy saving.
In addition, the present invention has a very strong antioxidant action on animal-derived cells such as fibroblasts, and has an excellent antioxidant stress action in a suppressive action on cases of diseases involving oxidative stress. An object is to provide an antioxidant stress agent.
Another object of the present invention is to provide a cell growth promoter having an excellent growth promoting action on animal-derived cells such as fibroblasts and excellent in promoting wound healing.
In addition, the present invention has high freshness retention for plants, seafood and the like, and enhances the physiological activity and immunity of seafood by using it for breeding and aquaculture of seafood such as freshwater and saltwater fish. It aims at providing the vitality enhancer which can be anticipated.
上記従来の課題を解決するために本発明の改質水及びそれを含有する抗酸化ストレス剤、細胞増殖促進剤並びに活力増強剤は、以下の構成を有している。
本発明の請求項１に記載の改質水は、原料水に外的刺激が加えられて改質された改質水であって、重クロロホルム中、内部基準にテトラメチルシランを使用して測定した１Ｈ−核磁気共鳴スペクトル（４００ＭＨｚ）が、次の（ａ）乃至（ｄ）の内いずれか１以上である構成を有している。
（ａ）主吸収ピークより低磁場側に、前記原料水には存在しなかった吸収ピークが出現したスペクトル
（ｂ）前記原料水の主吸収ピークより高磁場側に存在した少なくとも一つの吸収ピークが消失したスペクトル
（ｃ）前記原料水の主吸収ピークより高磁場側に存在した少なくとも一つの吸収ピークが低磁場側にシフトしたスペクトル
（ｄ）主吸収ピークの半値幅が、前記原料水の主吸収ピークの半値幅より増大したスペクトル
この構成により、以下のような作用が得られる。
（１）本発明者らは、１Ｈ−核磁気共鳴スペクトル（４００ＭＨｚ）に現れるように構造を変化させた水が抗酸化性を発現し、線維芽細胞等の動物由来の細胞に対する酸化ストレスの抑制、線維芽細胞等の動物由来の細胞に対する増殖促進作用、植物や魚介類等の活力増強、動物の整腸効果といった特異的な効果が得られることを見出し、本発明を完成させた。１Ｈ−核磁気共鳴スペクトル（４００ＭＨｚ）の主吸収ピークより低磁場側に原料水には存在しなかった吸収ピークが出現したり、主吸収ピークの半値幅が原料水の主吸収ピークの半値幅より増大したことは、プロトンの数が増えたことを示している。これは水素結合性が強くなった、又は水素結合数が多くなった状態であり、強固で大きな水分子集団が形成され水分子が構造化された結果であると推察される。このような性質の改質水が酸化剤を取り囲めば、酸化剤が他の物質から電子を奪って酸化させる酸化反応を抑制することができ、改質水は抗酸化性を発現する。
（２）原料水の主吸収ピークより高磁場側に存在した吸収ピークが低磁場側にシフトしたり消失したりすることは、電子が水素の原子核を遮蔽する度合が原料水より改質水のほうが小さくなっており、水素の原子核の回りの電子密度が減少していることを示している。このため強固で大きな水分子集団が形成され易くなるので、このような性質の改質水が酸化剤を取り囲めば、酸化剤が他の物質から電子を奪って酸化させる酸化反応を抑制することができ、改質水は抗酸化性を発現する。
（３）水の構造を変化させて抗酸化性を付与しているので、改質水を摂取した人体が負担を受けることなく、線維芽細胞等の動物由来の細胞に対する酸化ストレスの抑制、線維芽細胞等の動物由来の細胞に対する増殖促進作用、植物や魚介類等の活力増強や鮮度の保持、動物の整腸効果といった特異的な効果を得ることができる。
（４）原料水に外的刺激が加えられて改質されているので、水の電気分解等による改質処理のような多大なエネルギーを必要とせず、省エネルギー性に優れる。
In order to solve the above conventional problems, the modified water of the present invention and the antioxidant stress agent, cell growth promoter and vitality enhancer containing the same have the following constitutions.
The reformed water according to
(A) Spectrum in which an absorption peak that did not exist in the raw material water appears on the lower magnetic field side than the main absorption peak (b) At least one absorption peak present on the higher magnetic field side than the main absorption peak of the raw material water Disappeared spectrum (c) Spectrum in which at least one absorption peak present on the higher magnetic field side than the main absorption peak of the raw material water is shifted to the lower magnetic field side (d) The half width of the main absorption peak is the main absorption of the raw water The spectrum increased beyond the half-width of the peak. With this configuration, the following effects can be obtained.
(1) The present inventors show that the water whose structure has been changed so as to appear in the 1 H-nuclear magnetic resonance spectrum (400 MHz) expresses antioxidative properties, and exhibits oxidative stress on animal-derived cells such as fibroblasts. It has been found that specific effects such as suppression, growth promoting action on animal-derived cells such as fibroblasts, enhancement of vitality of plants and fish and shellfish, etc., and intestinal regulation of animals can be obtained, and the present invention has been completed. An absorption peak that did not exist in the raw water appears on the lower magnetic field side than the main absorption peak of 1 H-nuclear magnetic resonance spectrum (400 MHz), or the half width of the main absorption peak is the half width of the main absorption peak of the raw water. More increase indicates an increase in the number of protons. This is a state in which the hydrogen bondability is increased or the number of hydrogen bonds is increased, and it is presumed that this is a result of forming a strong and large water molecule group and structuring the water molecules. If the modified water having such a property surrounds the oxidizing agent, it is possible to suppress an oxidation reaction in which the oxidizing agent takes electrons from other substances and oxidizes, and the modified water exhibits antioxidant properties.
(2) The absorption peak existing on the high magnetic field side from the main absorption peak of the raw material water is shifted to the low magnetic field side or disappears. The degree to which electrons shield the hydrogen nuclei is higher than that of the raw water. Is smaller, indicating that the electron density around the hydrogen nucleus is decreasing. For this reason, a strong and large water molecule group is likely to be formed, so if the modified water having such properties surrounds the oxidant, the oxidant suppresses the oxidation reaction that oxidizes by taking electrons from other substances. The modified water exhibits antioxidant properties.
(3) Since the structure of water is changed to impart antioxidant properties, the human body ingesting the modified water is not burdened, suppressing oxidative stress on animal-derived cells such as fibroblasts, fibers Specific effects such as a growth promoting action on animal-derived cells such as blast cells, enhancement of vitality of plants and fish and shellfishes, maintenance of freshness, and an intestinal effect of animals can be obtained.
(4) Since the raw material water is reformed by applying external stimulus, it does not require a large amount of energy as in the reforming process by water electrolysis and the like, and is excellent in energy saving.
ここで、原料水としては、改質水の用途に応じて、水道水，イオン交換水，蒸留水，工業用水，雨水，地下水，河川水，生活排水，産業排水，海水及びそれら由来の水から選択された少なくとも１種が用いられる。
飲料水として用いられるときは、水道水や地下水等が好適に用いられる。点滴，注射，透析，創傷治癒等の医療用や化粧料として用いられるときは、イオン交換水や蒸留水等が好適に用いられる。動物や植物の活力増強剤として用いられるときは、動物や植物の種類に応じて、工業用水，雨水，河川水，生活排水，産業排水，海水等が好適に用いられる。必要に応じ、濾過や浄化等を行った後、用いることもできる。
Here, as raw material water, tap water, ion exchange water, distilled water, industrial water, rainwater, groundwater, river water, domestic wastewater, industrial wastewater, seawater and water derived from them, depending on the use of reformed water At least one selected is used.
When used as drinking water, tap water or groundwater is preferably used. When used for medical purposes such as infusion, injection, dialysis, wound healing, and cosmetics, ion-exchanged water or distilled water is preferably used. When used as a vitality enhancer for animals and plants, industrial water, rainwater, river water, domestic wastewater, industrial wastewater, seawater and the like are suitably used depending on the type of animal or plant. If necessary, it can be used after filtration or purification.
原料水に加えられる外的刺激としては、例えば、原料水中でセラミック粒子を流動させ衝突させる処理、磁場処理、原料水に高圧の静電場を作用させる電場処理、赤外線処理、紫外線処理などを用いることができ、原料水に他の成分を溶解させたり、直流電圧や交流電圧を印加して原料水から還元水を得る処理方法でなければ、特に制限を受けるものではない。 As an external stimulus applied to the raw material water, for example, a treatment that causes ceramic particles to flow and collide in the raw water, a magnetic field treatment, an electric field treatment that causes a high-voltage electrostatic field to act on the raw water, an infrared treatment, an ultraviolet treatment, etc. There is no particular limitation as long as it is not a treatment method in which other components are dissolved in the raw water or a reduced voltage is obtained from the raw water by applying a DC voltage or an AC voltage.
１Ｈ−核磁気共鳴スペクトルの測定条件としては、測定温度は２５℃〜３０℃であればいずれの温度を選択しても構わない。測定モード；single pulse、パルス幅；５．６μｓ（４５°）、パルス待時間；５．０ｓｅｃ、積算回数；８回以上、ケミカルシフト；基準物質として、テトラメチルシランを少量添加した重クロロホルム溶液を２重管外側に用い、テトラメチルシランのプロトンシグナルを基準ピーク(０ｐｐｍ) とし、相対的位置で表す。内部管に測定水を導入する。測定装置は４００ＭＨｚで測定できる機器であればメーカーは問わない。例えば、ＪＥＯＬ製ＪＮＭ−ＥＣＡ４００が用いられる。 As measurement conditions for the 1 H-nuclear magnetic resonance spectrum, any temperature may be selected as long as the measurement temperature is 25 ° C. to 30 ° C. Measurement mode: single pulse, pulse width: 5.6 μs (45 °), pulse waiting time: 5.0 sec, integration count: 8 times or more, chemical shift; deuterated chloroform solution with a small amount of tetramethylsilane added as a reference substance Used on the outside of the double tube, the proton signal of tetramethylsilane is taken as the reference peak (0 ppm) and expressed in relative position. Introduce measurement water into the inner pipe. Any manufacturer can be used as long as the measuring device can measure at 400 MHz. For example, JNM-JNM-ECA400 is used.
１Ｈ−核磁気共鳴スペクトル（４００ＭＨｚ）が、前記（ａ）〜（ｄ）のスペクトルの内いずれか１以上を有していればよいが、特に、前記（ａ）又は（ｄ）のスペクトルであることが好ましい。前記（ａ）又は（ｄ）のスペクトルが現れるのは、改質水の水素結合性が強くなった、又は水素結合数が多くなった状態であると推察されるため、このようなスペクトルを有する水分子が酸化剤を取り囲むことにより、酸化剤の働きが弱められ抗酸化性を付与できると推察されるからである。 The 1 H-nuclear magnetic resonance spectrum (400 MHz) may have any one or more of the spectra (a) to (d), and in particular, the spectrum (a) or (d). Preferably there is. The spectrum of (a) or (d) appears because the hydrogen bondability of the reformed water has been increased or the number of hydrogen bonds has been increased. This is because it is presumed that the water molecule surrounds the oxidizing agent, thereby reducing the function of the oxidizing agent and imparting antioxidant properties.
また、１Ｈ−パルス核磁気共鳴のＣＰＭＧ法における改質水のスピン−スピン緩和時間（Ｔ２）が、原料水のスピン−スピン緩和時間（Ｔ２）に対して５〜４０％好ましくは７〜３０％短縮されているのが好ましい。スピン−スピン緩和時間（Ｔ２）が短縮したことは、強固で大きな水分子集団が形成されたことを示していると推察されるため、このような性質の改質水が酸化剤を取り囲めば、酸化剤による酸化反応を抑制することができ抗酸化性を発現するからである。
なお、スピン−スピン緩和時間（Ｔ２）の短縮率（原料水の緩和時間をＴａ、改質水の緩和時間をＴｂとしたときの（Ｔａ−Ｔｂ）／Ｔａを短縮率とする）が７％より小さくなるにつれ、原料水が十分改質されておらず抗酸化性が乏しくなる傾向がみられ、３０％を超えるにつれ長時間の改質処理時間や改質操作を要し改質水の処理効率が低下する傾向がみられる。特に、５％未満になるか４０％を超えると、これらの傾向が著しいためいずれも好ましくない。
Further, 1 H- reforming water of the spin in the CPMG method of pulse nuclear magnetic resonance - spin relaxation time (T 2) is, the raw water spin - 5-40% preferably relative
The shortening rate of the spin-spin relaxation time (T 2 ) (when the relaxation time of the raw material water is Ta and the relaxation time of the reforming water is Tb, (Ta−Tb) / Ta is the shortening rate) is 7. As the water content becomes smaller than 30%, the raw material water is not sufficiently reformed and there is a tendency for the antioxidant property to be poor. There is a tendency for processing efficiency to decrease. In particular, if it is less than 5% or exceeds 40%, these tendencies are remarkable, and none of them is preferable.
１Ｈ−パルス核磁気共鳴の測定条件としては、測定温度は２０〜３０℃であればいずれの温度を選択しても構わない。観測周波数；２５ＭＨｚ、測定モード；ＣＰＭＧ法（Carr-Purcell Meiboom-Gill method）、パルス幅；Ｐｗ１…２．１μｓ，Ｐｗ２…４．０μｓ、パルス待時間；１５秒、積算回数；４回、Ｌｏｏｐ（ＲＦパルスを連続して発生させるときの回数）；５００回とした。測定装置としてはパルス核磁気共鳴装置、例えばＪＥＯＬ製ＪＮＭ−ＭＵ２５Ａが用いられる。 As measurement conditions for 1 H-pulse nuclear magnetic resonance, any temperature may be selected as long as the measurement temperature is 20 to 30 ° C. Observation frequency: 25 MHz, measurement mode: CPMG method (Carr-Purcell Meiboom-Gill method), pulse width: Pw1... 2.1 .mu.s, Pw2... 4.0 .mu.s, pulse waiting time: 15 seconds, integration count: 4 times, Loop ( The number of times when RF pulses are continuously generated): 500 times. As the measuring apparatus, a pulse nuclear magnetic resonance apparatus, for example, JNM-MU25A manufactured by JEOL is used.
本発明の請求項２に記載の発明は、請求項１に記載の改質水であって、前記原料水に加えられた前記外的刺激が、複数のセラミック粒子を前記原料水の中で流動させ衝突させる構成を有している。
この構成により、請求項１で得られる作用に加え、以下のような作用が得られる。
（１）はっきりとした理由は不明であるが、複数のセラミック粒子を原料水の中で流動させ衝突させることで水を活性化し改質することができるので、電解処理のような多大なエネルギーが不要であり、またセラミック粒子は繰り返し使用できるので、改質水の製造コストを抑えることができ改質水の生産性に優れる。
The invention according to
With this configuration, in addition to the operation obtained in the first aspect, the following operation can be obtained.
(1) Although the reason is not clear, it is possible to activate and modify water by causing a plurality of ceramic particles to flow and collide with each other in raw material water. Since the ceramic particles can be used repeatedly, the production cost of the reforming water can be suppressed and the reforming water productivity is excellent.
ここで、セラミック粒子としては、シリカ、アルミナ、アルカリ金属，アルカリ土類金属等の酸化物を含有する酸化物系鉱石粉末を、粒状に造粒し焼成して製造したものが用いられる。
シリカとアルミナの割合は、シリカ１００重量部に対しアルミナが１０〜９５重量部好ましくは２０〜８０重量部が好適に用いられる。アルミナが２０重量部より少なくなるにつれセラミック粒子の機械的強度が低下する傾向がみられ、８０重量部より多くなるにつれ、所定の機械的強度を得るのに必要な焼成温度が高くなり省エネルギー性に欠ける傾向がみられる。特に、１０重量部より少なくなるか９５重量部より多くなると、これらの傾向が著しくなるためいずれも好ましくない。
Here, as the ceramic particles, those produced by granulating and firing oxide-based ore powder containing oxides such as silica, alumina, alkali metal, alkaline earth metal and the like are used.
The ratio of silica to alumina is suitably 10 to 95 parts by weight, preferably 20 to 80 parts by weight, with respect to 100 parts by weight of silica. As alumina becomes less than 20 parts by weight, the mechanical strength of the ceramic particles tends to decrease, and as it becomes more than 80 parts by weight, the firing temperature necessary to obtain a predetermined mechanical strength increases, resulting in energy savings. There is a tendency to lack. In particular, when the amount is less than 10 parts by weight or more than 95 parts by weight, these tendencies become remarkable, so that neither is preferable.
また、セラミック粒子は、ＢＥＴ法による比表面積が０．０１〜１ｍ２／ｇ、かつ、見掛け密度が１．６〜３．５ｇ／ｃｍ３、粒径が０．１〜１０ｍｍに形成されるのが好適である。
比表面積が０．０１ｍ２／ｇより小さくなるにつれ原料水との接触面積が減少して改質効率が低下する傾向がみられ、１ｍ２／ｇより大きくなるにつれセラミック粒子が脆弱になり粒子同士の衝突の際に磨耗し易く耐久性が低下する傾向がみられる。
見掛け密度が１．６ｇ／ｃｍ３より小さくなるにつれ、粒子の機械的強度が低下し粒子同士の衝突の際に磨耗したり欠けたりする傾向や、粒子同士が水中で衝突した後に沈降し難く流動し難くなる傾向がみられ、３．５ｇ／ｃｍ３より大きくなるにつれ沈降速度が速いため粒子が水中で流動し難く流動状態が低下し水の改質効果が低下する傾向がみられる。
粒径が０．１ｍｍより小さくなるにつれ、流動するセラミック粒子の運動エネルギーが小さいためセラミック粒子が衝突した際の改質効果が低下する傾向がみられ、１０ｍｍより大きくなるにつれ、セラミック粒子を流動化させるのに必要なエネルギーが増加するとともに、粒子同士の衝突点が少なくなり粒子の衝突による改質効果が低下する傾向がみられる。
The ceramic particles are formed to have a specific surface area of 0.01 to 1 m 2 / g, an apparent density of 1.6 to 3.5 g / cm 3 , and a particle size of 0.1 to 10 mm according to the BET method. Is preferred.
As the specific surface area becomes smaller than 0.01 m 2 / g, the contact area with the raw material water tends to decrease and the reforming efficiency tends to decrease, and as the specific surface area becomes larger than 1 m 2 / g, the ceramic particles become brittle and become particles. There is a tendency for durability to deteriorate during wear.
As the apparent density becomes smaller than 1.6 g / cm 3 , the mechanical strength of the particles decreases, and the particles tend to wear or chip when they collide with each other. Since the sedimentation rate is faster as it becomes larger than 3.5 g / cm 3, the particles are less likely to flow in water, so that the flow state is lowered and the water reforming effect is lowered.
As the particle size becomes smaller than 0.1 mm, the kinetic energy of the flowing ceramic particles is small, so the modification effect when the ceramic particles collide tends to decrease. As the particle size becomes larger than 10 mm, the ceramic particles become fluidized. There is a tendency that the energy required to make the particles increase, the number of collision points between particles decreases, and the modification effect due to particle collision decreases.
次に、セラミック粒子を用いた改質水の製造方法を説明する。適量の複数のセラミック粒子をポリエチレン製，ポリエチレンテレフタレート製等の合成樹脂製，ガラス製，ステンレス製等の容器に入れ、これに処理すべき原料水をいれ、数回から多くても十数回強く振るなどして、セラミック粒子と原料水とを接触させ、またセラミック粒子を相互摩擦させ衝突させればよい。改質水は、容器から出してそのまま使用しても良いし、噴霧して用いても良い。
また、水道の元栓付近にセラミック粒子を充填した処理器を設置し、または水道蛇口開口部に該処理器を設置し、水流によってセラミック粒子を流動して相互摩擦させ衝突を起こさせ、セラミック粒子と水流界面の接触等を増やすことにより、改質水を製造することができる。処理器への水の導入方向は、原則として下方から上方へ向かう方向が望ましいが、水中で連続してセラミック粒子が流動し相互摩擦され衝突する水流環境が達成されていれば、どの方向より導入し取り出しても構わない。例えば、処理器の中にセラミック粒子を十分流動できる空所を設けて装填した上昇流動層方式、層内でセラミック粒子と原料水とを強制的に流動衝突させる強制循環方式等が用いられる。また、処理器の原料水流入部に整流板を設置したり、改質水の流出部の大きさ，形状，それらの位置を適宜選択することにより、原料水を渦流にしたり乱流にしたりするなどの方法も有効である。
Next, a method for producing reformed water using ceramic particles will be described. Put an appropriate amount of multiple ceramic particles in a polyethylene, polyethylene terephthalate or other synthetic resin, glass, or stainless steel container, and put the raw water to be processed into it. The ceramic particles and the raw water may be brought into contact with each other by shaking, and the ceramic particles may be rubbed against each other to collide with each other. The reformed water may be taken out from the container and used as it is, or may be used by spraying.
Also, a processing device filled with ceramic particles is installed near the main tap of the water supply, or the processing device is installed at the opening of the water faucet. The reformed water can be produced by increasing the contact at the water flow interface. In principle, the direction of water introduction to the processor is preferably from the bottom to the top, but from any direction as long as a water flow environment in which ceramic particles continuously flow and rub against each other in water is achieved You can take it out. For example, an ascending fluidized bed method in which a void capable of sufficiently flowing ceramic particles is provided in a processing unit, a forced circulation method in which ceramic particles and raw water are forced to collide with each other in the layer, and the like are used. In addition, the rectifying plate is installed at the raw material water inflow part of the processor, or the raw material water is made vortex or turbulent by appropriately selecting the size, shape and position of the reforming water outflow part. Such a method is also effective.
以上のようにセラミック粒子を水雰囲気下で流動、相互摩擦、衝突させることにより、溶出成分の全くないセラミック粒子と接した水は、界面動電現象により、水に正電荷を与え、その水素結合形成能や、分極性能、不対電子を持つ物質との相互作用能力、パイ電子共役系を持つ物質などとの相互作用能力等の特性が大きく変化し、又は向上する。その結果、セラミック粒子で処理された水の１Ｈ−ＮＭＲスペクトルは、処理前の原料水と比較して明確な違いを生ずる。即ち、水素結合力が増加した、より構造化された改質水に変貌する。 As described above, when the ceramic particles flow, interact with each other and collide with each other in a water atmosphere, the water in contact with the ceramic particles having no elution component gives a positive charge to the water due to the electrokinetic phenomenon, and its hydrogen bonding. Characteristics such as forming ability, polarization performance, ability to interact with a substance having unpaired electrons, ability to interact with a substance having a pi-electron conjugated system, etc. are greatly changed or improved. As a result, the 1 H-NMR spectrum of water treated with ceramic particles produces a distinct difference compared to the raw water before treatment. That is, the water is transformed into a more structured reformed water having an increased hydrogen bonding force.
なお、セラミック粒子が流動する速度を０．０３〜０．２ｍ／ｓにすると、セラミック粒子による水の改質効果を高めることができ好ましい。流動するセラミック粒子の速度が０．０３ｍ／ｓ未満になるか、０．２ｍ／ｓより速くなると、原料水に対する核磁気共鳴スペクトルや核磁気共鳴の緩和時間の変化が小さくなり、水を活性化する効果が低下するためいずれも好ましくない。 In addition, it is preferable that the rate at which the ceramic particles flow is 0.03 to 0.2 m / s because the effect of water modification by the ceramic particles can be enhanced. When the velocity of the flowing ceramic particles is less than 0.03 m / s or faster than 0.2 m / s, the change of the nuclear magnetic resonance spectrum and the relaxation time of the nuclear magnetic resonance with respect to the raw water becomes small and the water is activated. Neither is preferable because the effect of the reduction is reduced.
本発明の請求項３に記載の動物由来の細胞に対する抗酸化ストレス剤は、請求項１又は２に記載の改質水を主成分として含有した構成を有している。
この構成により、以下のような作用が得られる。
（１）改質水を主成分としているので、線維芽細胞などの動物由来の細胞に対し非常に強い抗酸化作用を有し、酸化ストレスが関与する疾患の症例に対して抑制的に作用し優れた抗酸化ストレス作用を有する。
The antioxidant stress agent for animal-derived cells according to
With this configuration, the following effects can be obtained.
(1) Because it contains modified water as the main component, it has a very strong antioxidant effect on animal-derived cells such as fibroblasts, and acts in a suppressive manner on diseases involving oxidative stress. Has excellent antioxidant stress action.
ここで、抗酸化ストレス剤としては、主成分として改質水を含有していればよく、全部が改質水で構成されていてもよい。
抗酸化ストレス剤は、直接体外から作用させる、点滴などの溶媒として用いる、飲料水として用いるなどの方法で摂取することができ、飲用、点滴用、注射用、透析用、創傷治癒用、手術用、化粧料等として用いることができる。体外から作用させた場合には、改質水は浸透性にも優れるため、皮膚に浸透して酸化刺激に常に晒されている皮膚の老化の予防やアトピー性皮膚炎の改善が期待される。また、皮膚のメラニン色素沈着の防止，改善など、ビタミンＣの効能と同様の効果が期待される。体内での作用としては酸化ストレスが重要な病因の一つであると言われている動脈硬化，肺気腫，肺繊維症，喘息，糖尿病，すべての臓器の虚血再環流障害，白内障及び発癌の抑制，あるいは高圧酸素療法における副作用の防止に効果があると考えられる。化粧料用としては、改質水の低刺激性・保水性をその主たる効能とする化粧水などの分野で優れた効果を発揮することが期待される。
Here, the antioxidant stress agent only needs to contain reformed water as a main component, and all may be composed of the reformed water.
Antioxidant stress agents can be taken directly from outside the body, used as a solvent for infusions, used as drinking water, etc., and can be ingested for drinking, infusion, injection, dialysis, wound healing, and surgery. It can be used as cosmetics. When it is applied from outside the body, the modified water is excellent in permeability, so that it is expected to prevent skin aging and improve atopic dermatitis that penetrates the skin and is constantly exposed to oxidative stimuli. Moreover, the effect similar to the effect of vitamin C, such as prevention and improvement of melanin pigmentation of skin, is expected. Suppression of arteriosclerosis, pulmonary emphysema, pulmonary fibrosis, asthma, diabetes, ischemic reperfusion injury of all organs, cataract and carcinogenesis, which is said to be one of the important etiological factors for oxidative stress It may be effective in preventing side effects in hyperbaric oxygen therapy. For cosmetics, it is expected to exhibit excellent effects in fields such as skin lotion, which has low efficacy and water retention as the main effect.
飲用、点滴用、注射用、透析用、創傷治癒用、手術用、化粧料等の用途に応じ、抗酸化ストレス剤には、改質水の他、例えば、アルコール等の親水性溶剤、ポリエチレングリコール等の溶解補助剤、増粘剤、等張化剤、緩衝化剤、保存剤等を加えることができる。 Antioxidant stress agents include modified water, hydrophilic solvents such as alcohol, polyethylene glycol, etc., depending on the application such as drinking, infusion, injection, dialysis, wound healing, surgery, cosmetics, etc. A solubilizer such as a solubilizer, a thickener, an isotonic agent, a buffering agent, a preservative, and the like can be added.
本発明の請求項４に記載の動物由来の細胞に対する細胞増殖促進剤は、請求項１又は２に記載の改質水を主成分として含有した構成を備えている。
この構成により、以下のような作用が得られる。
（１）改質水は線維芽細胞等の動物由来の細胞に対する増殖促進作用を有しているので、創傷治癒の促進等に優れる。
The cell growth promoter for animal-derived cells according to
With this configuration, the following effects can be obtained.
(1) Since the modified water has a growth promoting action on animal-derived cells such as fibroblasts, it is excellent in promoting wound healing.
ここで、細胞増殖促進剤としては、主成分として改質水を含有していればよく、全部が改質水で構成されていてもよい。
細胞増殖促進剤は、直接体外から作用させる、点滴などの溶媒として用いる、飲料水として用いるなどの方法で摂取することができ、飲用、点滴用、注射用、透析用、創傷治癒用、手術用、化粧料等として用いることができる。
細胞増殖促進剤には、改質水の他、用途に応じ、例えば増粘剤、等張化剤、緩衝化剤、保存剤等を加えることができる。
Here, as the cell growth promoter, it is sufficient if it contains modified water as a main component, and all may be composed of modified water.
Cell growth promoter can be ingested by methods such as direct action from outside the body, used as a solvent for infusion, etc., used as drinking water, etc. for drinking, infusion, injection, dialysis, wound healing, surgery It can be used as cosmetics.
In addition to the modified water, for example, a thickener, an isotonic agent, a buffering agent, a preservative and the like can be added to the cell growth promoter according to the intended use.
本発明の請求項５に記載の活力増強剤は、請求項１又は２に記載の改質水を主成分として含有した構成を有している。
この構成により、以下のような作用が得られる。
（１）強い抗酸化作用を有する改質水を主成分としているので、植物や魚介類に対して活力増強作用を有し、収穫後の植物に対して高い鮮度保持性を有する。
The vitality enhancer according to
With this configuration, the following effects can be obtained.
(1) Since the main component is modified water having a strong antioxidant action, it has a vitality enhancing action on plants and fish and shellfish, and has a high freshness retaining property on harvested plants.
ここで、活力増強剤としては、主成分として改質水を含有していればよく、全部が改質水で構成されていてもよい。
活力増強剤としては、養液栽培の植物体に与えると植物体の活力を高めることができ、収穫後の植物体の水分の保持力を高め鮮度を長期間維持できる。また、切花等の植物体に吸水させることで植物体を長持ちさせることができる。また、活魚・軟体動物・甲殻類等の魚介類の飼育・養殖や、運搬したり保存したりするときの製氷用や浸漬用の液体として用いることで、魚介類を衰弱させることなく生育させ輸送することができる。
なお、活力増強剤には、用途に応じ、改質水の他、肥料、栄養剤等を加えることができる。
Here, as a vitality enhancer, it should just contain the reforming water as a main component, and all may be comprised with the reforming water.
As a vitality enhancer, when applied to a hydroponic plant, the vitality of the plant can be increased, the water retention of the plant after harvesting can be increased, and the freshness can be maintained for a long time. Moreover, a plant body can be made to last long by making a plant body, such as a cut flower, absorb water. In addition, it can be used as a liquid for ice making and soaking when transporting and storing live fish, mollusks, crustaceans, and other seafood, so that the seafood can be grown and transported without debilitating. can do.
In addition, a fertilizer, a nutrient, etc. can be added to a vitality enhancer other than modified water according to a use.
以上のように、本発明の改質水及びそれを含有する抗酸化ストレス剤、細胞増殖促進剤並びに活力増強剤によれば、以下のような有利な効果が得られる。
請求項１に記載の発明によれば、
（１）水の構造を変化させて抗酸化性を付与しているので、改質水を摂取した人体が負担を受けることなく、線維芽細胞等の動物由来の細胞に対する酸化ストレスの抑制、線維芽細胞等の動物由来の細胞に対する増殖促進作用、植物や魚介類等の鮮度の保持や活力増強、動物の整腸効果といった特異的な効果を得ることができ、植物鮮度保持効果、動物の整腸効果、発毛・増毛効果、線維芽細胞に対する酸化ストレスの抑制のみならず、酸化ストレスが関与する疾患、即ち、体外からの作用としては皮膚の老化予防、アトピー性皮膚炎の改善、皮膚のメラニン色素沈着の防止、改善などに、動脈硬化、肺気腫、肺繊維症、喘息、糖尿病、すべての臓器の虚血再環流障害、白内障及び発癌の抑制、あるいは、高圧酸素療法における副作用の防止などに効果があると考えられる改質水を提供することができる。
（２）原料水に外的刺激が加えられて改質されているので、水の電気分解等による改質処理のような多大なエネルギーを必要とせず、省エネルギー性に優れた改質水を提供することができる。
As described above, according to the modified water of the present invention and the antioxidant stress agent, cell growth promoter and vitality enhancer containing the same, the following advantageous effects can be obtained.
According to the invention of
(1) Since the structure of water is changed to impart antioxidant properties, the human body ingesting the modified water is not burdened, suppressing oxidative stress on animal-derived cells such as fibroblasts, fibers Specific effects such as growth-promoting action on animal-derived cells such as blasts, maintenance of freshness and vitality of plants and seafood, and animal intestinal regulation can be obtained. Intestinal effect, hair growth / hair growth effect, not only suppression of oxidative stress on fibroblasts, but also diseases involving oxidative stress, that is, external effects include prevention of skin aging, improvement of atopic dermatitis, skin Prevention and improvement of melanin pigmentation, prevention of arteriosclerosis, emphysema, pulmonary fibrosis, asthma, diabetes, ischemia reperfusion injury of all organs, cataract and carcinogenesis, or prevention of side effects in hyperbaric oxygen therapy It is possible to provide a reforming water to be considered etc. is effective in.
(2) Since the raw water is reformed by applying external stimulus, it does not require a lot of energy like the reforming process by water electrolysis, etc., and provides reformed water with excellent energy saving can do.
請求項２に記載の発明によれば、請求項１の効果に加え、
（１）電解処理のような多大なエネルギーが不要であり、またセラミック粒子は繰り返し使用できるので、改質水の製造コストを抑えることができ生産性に優れた改質水を提供することができる。
According to invention of
(1) Since enormous energy such as electrolytic treatment is unnecessary and ceramic particles can be used repeatedly, the production cost of reforming water can be suppressed, and reforming water with excellent productivity can be provided. .
請求項３に記載の発明によれば、
（１）線維芽細胞などの動物由来の細胞に対し非常に強い抗酸化作用を有し、酸化ストレスが関与する疾患の症例に対して抑制的に作用し優れた抗酸化ストレス作用を有する抗酸化ストレス剤を提供することができる。
According to invention of
(1) Antioxidant having a very strong antioxidative effect on animal-derived cells such as fibroblasts, an inhibitory action on a disease case involving oxidative stress, and an excellent antioxidative stress effect A stress agent can be provided.
請求項４に記載の発明によれば、
（１）線維芽細胞等の動物由来の細胞に対する優れた増殖促進作用を有し、創傷治癒の促進等に優れた細胞増殖促進剤を提供することができる。
According to invention of
(1) It is possible to provide a cell growth promoting agent that has an excellent growth promoting action on animal-derived cells such as fibroblasts and is excellent in promoting wound healing.
請求項５に記載の発明によれば、
（１）植物や魚介類等に対して高い鮮度保持性を有し、淡水・海水魚等の魚介類の飼育、養殖などに使用することによって魚介類の生理活性及び免疫力増強をも期待できる活力増強剤を提供することができる。
According to the invention of
(1) It has high freshness retention for plants and seafood, etc., and can be expected to enhance the physiological activity and immunity of seafood by using it for breeding and aquaculture of seafood such as freshwater and saltwater fish. A vitality enhancer can be provided.
以下、本発明を実施例により具体的に説明する。なお、本発明はこれらの実施例に限定されるものではない。
（実施例１）
（セラミック粒子の製造）
SiO２：７０ｗｔ％、Al2O3：１１．９ｗｔ％、Na2O：４．７９ｗｔ％、CaO：２．４３ｗｔ％、Fe2O3：３．１９ｗｔ％、K2O：４．１７ｗｔ％、その他１ｗｔ％未満のMg、Cu、Snなどを含む天然鉱石を粒径８μｍ以下に微粉砕し、この微粉末をエタノールと共にボールミルにいれ、転動し、更に、微粉化と同時に混練した。この混練物をピン回転式造粒装置で遠心力が１００００Ｇとなる条件で１〜２時間回転させ、エタノールを揮発させながら球形に造粒成形した。ピン回転式造粒装置とは、混練物を所定サイズの粒子に造粒する装置である。この造粒物を耐熱容器に充填して電気炉に入れ、空気雰囲気下又は不活性ガス雰囲気中で１１５０〜１１９０℃焼結した。得られた焼結体は、褐色光沢を有する硬質の粒径約３ｍｍの球状粒子であり、見掛け密度は２．３ｇ／ｃｍ３であった。また、その表面の比表面積はBET法で０．４７m２／ｇであった。
Hereinafter, the present invention will be specifically described by way of examples. The present invention is not limited to these examples.
Example 1
(Manufacture of ceramic particles)
SiO 2: 70wt%, Al 2 O 3: 11.9wt%, Na 2 O: 4.79wt%, CaO: 2.43wt%, Fe 2 O 3: 3.19wt%, K 2 O: 4.17wt% In addition, natural ore containing Mg, Cu, Sn, etc. of less than 1 wt% was finely pulverized to a particle size of 8 μm or less, and this fine powder was placed in a ball mill with ethanol, rolled, and kneaded simultaneously with pulverization. This kneaded product was rotated by a pin rotary granulator for 1 to 2 hours under the condition that the centrifugal force was 10,000 G, and granulated and formed into a spherical shape while volatilizing ethanol. A pin rotary granulator is an apparatus that granulates a kneaded product into particles of a predetermined size. This granulated product was filled in a heat-resistant container, put in an electric furnace, and sintered at 1150 to 1190 ° C. in an air atmosphere or an inert gas atmosphere. The obtained sintered body was a hard spherical particle having a brown particle size and a particle size of about 3 mm, and the apparent density was 2.3 g / cm 3 . The specific surface area of the surface was 0.47 m 2 / g by the BET method.
（改質水の製造）
製造したセラミック粒子を、ガラス製スクリュー管瓶（５０ｍｌ）の内容積２５％となるように充填した。該スクリュー管瓶に原料水である純水を内容積６０％となるよう導入した後、蓋を閉め、セラミック粒子が水中で０．０３〜０．２ｍ／ｓの速度で流動し、相互摩擦、衝突するよう該スクリュー管瓶を数回振って改質水を得た。直ちに瓶の蓋を開けて改質水を採取し、採取直後の改質水と原料水の１Ｈ−核磁気共鳴スペクトル測定（４００ＭＨｚ）を実施した。
なお、原料水の純水は、香川県さぬき市の水道水を純水製造装置（ミリポア社製Milli-Q Academic A10）を用いて製造したものである。
核磁気共鳴スペクトルの測定装置はＪＥＯＬ製ＪＮＭ−ＥＣＡ４００、測定条件は測定温度；２５℃、測定モード；single pulse、パルス幅；５．６μｓ（４５°）、パルス待時間；５．０ｓｅｃ、積算回数；８回、ケミカルシフト；基準物質として、テトラメチルシランを少量添加した重クロロホルム溶液を２重管外側に用い、テトラメチルシランのプロトンシグナルを基準ピーク(０ｐｐｍ)、ロックシグナル；重水であった。
図１（ａ）は改質水の１Ｈ−核磁気共鳴スペクトルであり、図１（ｂ）は原料水の１Ｈ−核磁気共鳴スペクトルである。
原料水は、図１（ｂ）に示すように、４．６７ｐｐｍの位置にほぼ１本の主吸収ピークを示した。これに対し、改質水は、図１（ａ）に示すように、低磁場側である４．６７１ｐｐｍの位置に明確で大きな吸収ピークが新たに出現した。また、これらの吸収ピークが重なって、改質水の主吸収ピークの半値幅が、原料水の主吸収ピークの半値幅より増大した。このことは、改質水が水素結合性の強いまたは量的に水素結合数が多い状態に変化したことを示していると推察される。
(Production of reformed water)
The produced ceramic particles were filled so that the inner volume of a glass screw tube bottle (50 ml) was 25%. After introducing pure water as raw material into the screw tube bottle so as to have an internal volume of 60%, the lid is closed, and the ceramic particles flow in water at a speed of 0.03 to 0.2 m / s, mutual friction, The screw tube bottle was shaken several times so as to collide to obtain reformed water. Immediately after opening the bottle lid, the reformed water was collected, and 1 H-nuclear magnetic resonance spectrum measurement (400 MHz) of the reformed water and the raw material water immediately after collection was performed.
In addition, the pure water of raw material water is produced by using tap water from Sanuki City, Kagawa Prefecture, using a pure water production device (Milli-Q Academic A10 manufactured by Millipore).
The nuclear magnetic resonance spectrum measurement apparatus is JEOL JNM-ECA400, measurement conditions are measurement temperature: 25 ° C., measurement mode: single pulse, pulse width: 5.6 μs (45 °), pulse waiting time: 5.0 sec, number of
1A is a 1 H-nuclear magnetic resonance spectrum of the reformed water, and FIG. 1B is a 1 H-nuclear magnetic resonance spectrum of the raw water.
The raw water showed almost one main absorption peak at a position of 4.67 ppm as shown in FIG. On the other hand, as shown in FIG. 1 (a), a clear and large absorption peak newly appeared in the reformed water at the position of 4.671 ppm on the low magnetic field side. Moreover, these absorption peaks overlapped, and the half value width of the main absorption peak of the reformed water increased from the half value width of the main absorption peak of the raw material water. This is presumed to indicate that the reformed water has changed to a state in which hydrogen bonding is strong or quantitatively has a large number of hydrogen bonds.
（実施例２）
原料水を静岡県富士市の水道水を使用した以外は実施例1と同様にして、実施例２の改質水を製造した。改質水を製造した直後に瓶の蓋を開けて改質水を採取し、改質水と原料水の１Ｈ−核磁気共鳴スペクトルを測定した。測定は、外部標準としてＴＭＳクロロホルム溶液を２重管外側に用い、内部管に改質水または原料水を入れ、JEOL（株）製JNM-LA400を用いて行った。
測定条件は測定温度；２５℃、測定モード；single pulse、パルス幅；５．０μｓ（４５°）、パルス待時間；２．９０ｓｅｃ、積算回数；１００回、ケミカルシフト；ＴＭＳ−０ｐｐｍである。
図２（ａ）は改質水の１Ｈ−核磁気共鳴スペクトルであり、図２（ｂ）は原料水の１Ｈ−核磁気共鳴スペクトルである。
原料水は、図２（ｂ）に示すように、主吸収ピークが４．６７３ｐｐｍに、副吸収ピークが４．６７０ｐｐｍに、さらに高磁場側（４．６６２ｐｐｍ、４．６５８ｐｐｍ）に明確なショルダーピークが存在する。これに対し、改質水は、図２（ａ）に示すように、主吸収ピークより高磁場側にあったショルダーピークが消失し、４．６７３ｐｐｍ、４．６７０ｐｐｍの２つの吸収ピークを有するスペクトルに変化した。このことは、水素の原子核の回りの電子密度が減少していることを示している。このため強固で大きな水分子集団が形成され易く、水分子の構造化が容易な状態になったものと推察される。
(Example 2)
The reformed water of Example 2 was produced in the same manner as in Example 1 except that tap water from Fuji City, Shizuoka Prefecture was used as the raw material water. Immediately after the production of the reformed water, the bottle lid was opened to collect the reformed water, and 1 H-nuclear magnetic resonance spectra of the reformed water and the raw water were measured. The measurement was performed using a JMS-LA400 manufactured by JEOL Co., Ltd., using a TMS chloroform solution as an external standard on the outside of the double tube, putting the reformed water or raw material water in the internal tube.
Measurement conditions are measurement temperature: 25 ° C., measurement mode: single pulse, pulse width: 5.0 μs (45 °), pulse waiting time: 2.90 sec, number of integrations: 100 times, chemical shift: TMS-0 ppm.
FIG. 2A shows a 1 H-nuclear magnetic resonance spectrum of the reformed water, and FIG. 2B shows a 1 H-nuclear magnetic resonance spectrum of the raw water.
As shown in FIG. 2B, the raw water has a main absorption peak at 4.673 ppm, a secondary absorption peak at 4.670 ppm, and a clear shoulder peak on the high magnetic field side (4.662 ppm, 4.658 ppm). Exists. On the other hand, as shown in FIG. 2A, the reformed water has a spectrum having two absorption peaks of 4.673 ppm and 4.670 ppm, with the shoulder peak on the higher magnetic field side disappearing from the main absorption peak. Changed. This indicates that the electron density around the hydrogen nucleus is decreasing. For this reason, it is presumed that a strong and large water molecule group is easily formed, and that the water molecules are easily structured.
（実施例３）
実施例１で説明したセラミック粒子の製造方法と同様の方法で、粒径約２ｍｍの球状のセラミック粒子を製造した。このセラミック粒子１０ｇを容積が１００ｍｌのポリプロピレン製の容器に入れるとともに、「Milli-Q SP UFシステム（日本ミリポア株式会社製）」を使用して水道水から製造したミリポア水１００ｍｌを原料水として容器に入れた。密栓した後、セラミック粒子が水中で０．０３〜０．２ｍ／ｓの速度で流動するように１秒間に１回の頻度で容器を上下に大きく振とうして、セラミック粒子を衝突させ原料水を改質した。振とう前の原料水、振とう５秒後、１０秒後、６０秒後、３００秒後の改質水の１Ｈ−パルス核磁気共鳴のＣＰＭＧ法におけるスピン−スピン緩和時間（Ｔ２）を測定した（実施例）。
スピン−スピン緩和時間の測定は、パルス核磁気共鳴装置（ＪＥＯＬ製ＪＮＭ−ＭＵ２５Ａ）の試料管に、所定量の改質水または原料水を入れて行った。測定条件は、測定温度；３０℃、観測周波数；２５ＭＨｚ、測定モード；ＣＰＭＧ法、パルス幅；Ｐｗ１…２．１μｓ，Ｐｗ２…４．０μｓ、パルス待時間；１５秒、積算回数；４、Ｌｏｏｐ；５００であった。
比較のため、同じ大きさの容器にセラミック粒子を入れずに前述のミリポア水（原料水）だけを入れて振とうした場合の、３００秒後の水のスピン−スピン緩和時間（Ｔ２）も測定した（比較例）。
図３は実施例と比較例の振とう時間とスピン−スピン緩和時間との関係を示す図である。
スピン−スピン緩和時間（Ｔ２）の測定の結果、原料水の緩和時間（Ｔａ）は２．３５秒であったのに対し、実施例の改質水の緩和時間（Ｔｂ）は、振とう５秒後では２．１４秒であり、原料水の緩和時間に対する短縮率（（Ｔａ−Ｔｂ）／Ｔａ）は約９％であった。振とう３００秒後の改質水の緩和時間（Ｔｂ）は１．８２秒であり、原料水の緩和時間に対する短縮率は約２３％であった。一方、比較例では、３００秒後の緩和時間は２．２５秒であり、原料水の緩和時間に対する短縮率は、わずか４．３％であった。
なお、振とう５秒後の改質水の１Ｈ−核磁気共鳴スペクトルは図１（ａ）と同様のスペクトルであり、原料水の１Ｈ−核磁気共鳴スペクトルは図１（ｂ）と同様のスペクトルであった。また、振とう時間が増加するにつれ低磁場側にみられる吸収ピークが、より低磁場側にシフトする傾向がみられた。
以上のように本実施例によれば、改質水は１Ｈ−パルス核磁気共鳴のＣＰＭＧ法におけるスピン−スピン緩和時間（Ｔ２）を短縮させることができ、これは改質水が水素結合性の強いまたは量的に水素結合数が多い状態に変化し水分子が構造化されたことを示していると推察される。
(Example 3)
Spherical ceramic particles having a particle size of about 2 mm were produced in the same manner as the method for producing ceramic particles described in Example 1. While putting 10 g of the ceramic particles in a 100 ml polypropylene container, 100 ml of Millipore water produced from tap water using the “Milli-Q SP UF system (manufactured by Nihon Millipore Co., Ltd.)” is used as the raw water in the container. I put it in. After sealing, the container is shaken largely up and down once per second so that the ceramic particles flow in water at a speed of 0.03 to 0.2 m / s, and the ceramic particles collide with the raw material water. Modified. The raw water before shaking, the spin-spin relaxation time (T 2 ) in the CPMG method of 1 H-pulse nuclear magnetic resonance of the modified water after 5 seconds, 10 seconds, 60 seconds and 300 seconds after shaking. Measurement (Example).
The spin-spin relaxation time was measured by putting a predetermined amount of modified water or raw material water into a sample tube of a pulse nuclear magnetic resonance apparatus (JNM-MU25A manufactured by JEOL). Measurement conditions are: measurement temperature: 30 ° C., observation frequency: 25 MHz, measurement mode: CPMG method, pulse width: Pw1 ... 2.1 μs, Pw2 ... 4.0 μs, pulse waiting time: 15 seconds, number of integrations: 4, Loop; 500.
For comparison, the spin-spin relaxation time (T 2 ) of water after 300 seconds when the above-mentioned Millipore water (raw material water) alone is put in a container of the same size and shaken without adding ceramic particles is also shown. Measured (comparative example).
FIG. 3 is a graph showing the relationship between the shaking time and the spin-spin relaxation time in the examples and comparative examples.
As a result of the measurement of the spin-spin relaxation time (T 2 ), the relaxation time (Ta) of the raw material water was 2.35 seconds, whereas the relaxation time (Tb) of the modified water in the examples was shaken. After 5 seconds, it was 2.14 seconds, and the shortening rate ((Ta-Tb) / Ta) with respect to the relaxation time of the raw material water was about 9%. The relaxation time (Tb) of the reformed water after 300 seconds of shaking was 1.82 seconds, and the shortening rate with respect to the relaxation time of the raw material water was about 23%. On the other hand, in the comparative example, the relaxation time after 300 seconds was 2.25 seconds, and the shortening rate with respect to the relaxation time of the raw material water was only 4.3%.
The 1 H-nuclear magnetic resonance spectrum of the reformed water after 5 seconds of shaking is the same spectrum as in FIG. 1A, and the 1 H-nuclear magnetic resonance spectrum of the raw water is the same as in FIG. Spectrum. Further, as the shaking time increased, the absorption peak observed on the low magnetic field side tended to shift to the low magnetic field side.
As described above, according to this embodiment, the reformed water can shorten the spin-spin relaxation time (T 2 ) in the CPMG method of 1 H-pulse nuclear magnetic resonance, which is because the reformed water is bonded to hydrogen. It is inferred that the water molecule has been structured by changing to a state with a strong or quantitative hydrogen bond number.
（実施例４）
（改質水の製造）
水道水から製造した前述のミリポア水を原料水として、容積が１０００ｍｌのポリプロピレン製の容器に５００ｍｌ入れ、さらに実施例１で得られたセラミック粒子１０ｇを入れて密栓した後、セラミック粒子が水中で０．０３〜０．２ｍ／ｓの速度で流動するよう容器を上下に大きく２０回振とうすることで、セラミック粒子同士を衝突させ改質水を製造した。対照水としては、原料水のミリポア水を用いた。なお、改質水の１Ｈ−核磁気共鳴スペクトルは図１（ａ）と同様のスペクトルであり、原料水の１Ｈ−核磁気共鳴スペクトルは図１（ｂ）と同様のスペクトルであった。また、パルス核磁気共鳴装置（ＪＮＭ−ＭＵ２５Ａ）（ＣＰＭＧ法）を用いて改質水と原料水の１Ｈ−パルス核磁気共鳴のスピン−スピン緩和時間を測定し、原料水の緩和時間（Ｔａ）に対する改質水の緩和時間（Ｔｂ）の短縮率（（Ｔａ−Ｔｂ）／Ｔａ）を求めたところ、短縮率は１１％であった。
Example 4
(Production of reformed water)
Using the above-mentioned Millipore water produced from tap water as raw material water, 500 ml is put into a polypropylene container having a volume of 1000 ml, and further 10 g of ceramic particles obtained in Example 1 are put in a sealed cap. The container was shaken up and down 20 times so as to flow at a speed of 0.03 to 0.2 m / s to collide ceramic particles with each other to produce modified water. As control water, raw water Millipore water was used. The 1 H-nuclear magnetic resonance spectrum of the reformed water was the same as that in FIG. 1A, and the 1 H-nuclear magnetic resonance spectrum of the raw water was the same as that in FIG. In addition, the spin-spin relaxation time of 1 H-pulse nuclear magnetic resonance of reformed water and raw water was measured using a pulse nuclear magnetic resonance apparatus (JNM-MU25A) (CPMG method), and the relaxation time of raw water (Ta The shortening rate ((Ta-Tb) / Ta) of the relaxation time (Tb) of the reforming water with respect to) was found to be 11%.
（比較例１）
セラミック粒子が水中で０．０２ｍ／ｓ以下の速度で流動するように容器を緩やかに振とうした以外は実施例４と同様にして、比較例１の水を得た。比較例１の水の１Ｈ−核磁気共鳴スペクトルは図１（ｂ）と同様のスペクトルであった。また、スピン−スピン緩和時間の短縮率は２％であった。
(Comparative Example 1)
Comparative Example 1 water was obtained in the same manner as in Example 4 except that the container was gently shaken so that the ceramic particles flowed at a rate of 0.02 m / s or less in water. The 1 H-nuclear magnetic resonance spectrum of the water of Comparative Example 1 was the same as that shown in FIG. The shortening rate of the spin-spin relaxation time was 2%.
（比較例２）
セラミック粒子が水中で０．３ｍ／ｓ以上の速度で流動するように容器を激しく振とうした以外は実施例４と同様にして、比較例２の水を得た。比較例２の水の１Ｈ−核磁気共鳴スペクトルは図１（ｂ）と同様のスペクトルであった。また、スピン−スピン緩和時間の短縮率は２％であった。
(Comparative Example 2)
Comparative Example 2 water was obtained in the same manner as in Example 4 except that the container was vigorously shaken so that the ceramic particles flowed at a speed of 0.3 m / s or more in water. The 1 H-nuclear magnetic resonance spectrum of the water of Comparative Example 2 was the same as that shown in FIG. The shortening rate of the spin-spin relaxation time was 2%.
（細胞培養液の調製）
実施例４の改質水、比較例１，２の水及び対照水（原料水）に培養液粉末｛イーグルMEM培地粉末（Minimum Essential Medium）｝を溶解した後、オートクレーブにかけ滅菌し、改質水培養液、比較例１，２の培養液及び対照水培養液を作成した。
(Preparation of cell culture)
The culture solution powder {Eagle MEM medium powder (Minimum Essential Medium)} is dissolved in the modified water of Example 4, the water of Comparative Examples 1 and 2, and the control water (raw water), then sterilized by autoclaving, and the modified water. The culture solution, the culture solution of Comparative Examples 1 and 2, and the control water culture solution were prepared.
（細胞培養及び過酸化水素による酸化ストレスの負荷）
改質水培養液、比較例１，２の培養液及び対照水培養液を使用して線維芽細胞株であるTIG３−２０を培養した。
まず、９６wellの細胞培養プレートを用い、10,000個/wellの細胞を対照水培養液(200 μl)で２４時間培養し定着させた培養液を得た。次に、培養液を（１）対照水培養液のみ（対照水培地）、（２）対照水培養液に５０μＭの過酸化水素（Ｈ２Ｏ２）を加えたもの、（３）改質水培養液のみ（改質水培地）、（４）改質水培養液に５０μＭの過酸化水素（Ｈ２Ｏ２）を加えたもの、（５）比較例１の培養液に５０μＭの過酸化水素を加えたもの、（６）比較例２の培養液に５０μＭの過酸化水素を加えたものの６群（それぞれｎ＝１６）で培養した。２４，４８，７２時間後に細胞数の計測を行うため、それぞれのwellに青色色素である５ｍＭのMTT(3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide)を１０ μl加え、３７℃、４時間培養後、培養液を吸引除去し、DMSO(Dimethyl Sulfoxide) を100 μl 加え細胞を溶解し、室温で１０分間振とうした後、吸光度（570 nm）を測定した。測定した吸光度は統計処理し、ANOVA及びBonferroni検定を用いて多群間比較を行った。
図４は、改質水培地及び対照水培地への過酸化水素の負荷の有無と吸光度（２４時間後）との関係を示す図である。
生存している細胞は一定量のMTTを細胞内に取り込むため、生存細胞数と吸光度は正の直線的相関関係をもつ。このため、吸光度を測定し多群間比較を行うことで、改質水の細胞増殖作用や抗酸化作用が評価できる。
(Cell culture and oxidative stress caused by hydrogen peroxide)
The fibroblast cell line TIG3-20 was cultured using the modified water culture solution, the culture solutions of Comparative Examples 1 and 2 and the control water culture solution.
First, a 96-well cell culture plate was used to obtain a culture solution in which 10,000 cells / well were cultured and fixed in a control water culture solution (200 μl) for 24 hours. Next, the culture solution was (1) control water culture solution only (control water medium), (2) control water culture solution added with 50 μM hydrogen peroxide (H 2 O 2 ), and (3) modified water. Only the culture solution (modified water medium), (4) 50 μM hydrogen peroxide (H 2 O 2 ) added to the modified water culture solution, (5) 50 μM hydrogen peroxide to the culture solution of Comparative Example 1 (6) Culture was carried out in 6 groups (n = 16 for each) of 50 μM hydrogen peroxide in the culture solution of Comparative Example 2. To measure the number of cells after 24, 48 and 72 hours, each well contained 5 mM MTT (3- (4,5-Dimethyl-2-thiazolyl) -2,5-diphenyl-2H tetrazolium bromide, a blue dye. 10 μl), and after 4 hours of incubation at 37 ° C., the culture medium is removed by suction. 100 μl of DMSO (Dimethyl Sulfoxide) is added to lyse the cells, shaken at room temperature for 10 minutes, and then the absorbance (570 nm) It was measured. The measured absorbance was statistically processed and compared between multiple groups using ANOVA and Bonferroni test.
FIG. 4 is a graph showing the relationship between the presence or absence of hydrogen peroxide loading on the modified water medium and the control water medium and the absorbance (after 24 hours).
Since living cells take up a certain amount of MTT into the cell, the number of living cells and absorbance have a positive linear correlation. For this reason, the cell proliferation effect and antioxidant effect | action of reformed water can be evaluated by measuring a light absorbency and performing comparison between many groups.
（細胞増殖促進作用の評価）
図４に示すように、過酸化水素の負荷がない場合（Ｈ２Ｏ２（−））、２４時間培養後の吸光度は対照水培養液のみでは 0.225 ± 0.025であり、改質水培養液では0.323 ± 0.048であり、有意差をもって（p<0.01）改質水培養液のほうが高く、改質水に細胞増殖促進作用のあることが示された。
（抗酸化作用の評価）
過酸化水素を負荷した場合（Ｈ２Ｏ２（＋））、対照水培養液では、ほとんどの細胞が死滅しているのが顕微鏡下で観察され、吸光度も 図４に示すように、0.022 ± 0.014 と極めて低くなった。同様に、比較例１及び比較例２の培養液に過酸化水素を加えたものでも、ほとんどの細胞が死滅しているのが顕微鏡下で観察された。一方、改質水培養液では多くの細胞が生き残り、吸光度は 0.243 ± 0.131 と対照水培養液に過酸化水素を負荷した場合に比して、有意に（p<0.001）高値であった。改質水培養液に過酸化水素を負荷した場合（改質水（Ｈ２Ｏ２（＋））、対照水培養液で過酸化水素を負荷しない群（対照水（Ｈ２Ｏ２（−））との間には有意差が認められなかった。なお、４８時間及び７２時間の培養でも同様の結果が得られた。
以上のように、本実施例によれば、改質水は優れた抗酸化ストレス作用と細胞増殖促進作用を有していることが明らかになった。
(Evaluation of cell growth promoting effect)
As shown in FIG. 4, when there is no hydrogen peroxide load (H 2 O 2 (−)), the absorbance after 24 hours of culture is 0.225 ± 0.025 in the control water culture alone, and in the modified water culture. 0.323 ± 0.048, with a significant difference (p <0.01), the modified water culture medium was higher, indicating that the modified water has a cell growth promoting effect.
(Evaluation of antioxidant effect)
When hydrogen peroxide was loaded (H 2 O 2 (+)), most cells were observed to be dead under the microscope in the control water culture, and the absorbance was 0.022 ± as shown in FIG. It was extremely low at 0.014. Similarly, even when hydrogen peroxide was added to the culture solutions of Comparative Example 1 and Comparative Example 2, it was observed under a microscope that most cells were dead. On the other hand, many cells survived in the modified water culture, and the absorbance was 0.243 ± 0.131, which was significantly higher (p <0.001) than when the control water culture was loaded with hydrogen peroxide. When hydrogen peroxide is loaded on the reformed water culture solution (modified water (H 2 O 2 (+)), the group not loaded with hydrogen peroxide in the control water culture solution (control water (H 2 O 2 (−)) No significant difference was observed between the culturing time and the culturing time of 48 hours and 72 hours.
As described above, according to this example, it was revealed that the modified water has an excellent antioxidant stress action and cell growth promoting action.
（実施例５）
（細胞増殖能の評価）
水道水から製造した前述のミリポア水に培養液粉末｛イーグルMEM培地粉末（Minimum Essential Medium）｝を溶解した後、オートクレーブにかけ滅菌したものに、濃度１０％になるようにウシ胎児血清を加えた細胞培養液を作成した。この細胞培養液を用いて、線維芽細胞株であるＴＩＧ３−２０を培養した。まず、９６wellの細胞培養プレートを用い、5,000個/wellの細胞を細胞培養液（200μl）で２４時間培養し定着させた。
次に、細胞を対照水培養液と改質水培養液に分けて培養（それぞれ、ｎ＝１６）を開始し、２４，４８，７２，９６，１２０，１２４時間後に細胞数の増加を比較した。なお、対照水培養液は、水道水から製造した該ミリポア水に培養液粉末｛イーグルMEM培地粉末（Minimum Essential Medium）｝を溶解した後、オートクレーブにかけ滅菌した培養液であり、改質水培養液は、実施例４で得た改質水に同様の培養液粉末を溶解した後、オートクレーブにかけ滅菌した培養液である。
細胞数は血球計算板を用いて算定した。なお、細胞が弱って死滅することを避けるため、培養開始７２時間後に、対照水培養液及び改質水培養液をそれぞれ新しいものと交換した。
図５は、改質水培養液及び対照水培養液で培養した細胞数の経時変化を示す図である。
図５から明らかなように、培養開始７２時間後より、改質水培養液で培養した実施例の線維芽細胞数が、比較例の対照水培養液で培養した線維芽細胞数より有意に高く（p<0.01）、改質水に細胞増殖促進作用のあることが示された。
以上のように、本実施例によれば、改質水は優れた細胞増殖促進作用を有していることが明らかになった。このことから、改質水は創傷治癒の促進作用や細胞の生存環境の改善作用が期待できることがわかった。
(Example 5)
(Evaluation of cell proliferation ability)
Cells prepared by dissolving the culture powder {Eagle MEM medium powder (Minimum Essential Medium)} in the aforementioned Millipore water produced from tap water, sterilized by autoclaving, and adding fetal bovine serum to a concentration of 10% A culture solution was prepared. Fibroblast cell line TIG3-20 was cultured using this cell culture medium. First, using a 96-well cell culture plate, 5,000 cells / well were cultured and fixed in a cell culture medium (200 μl) for 24 hours.
Next, the cells were divided into a control water culture solution and a modified water culture solution, and culture (n = 16, respectively) was started. After 24, 48, 72, 96, 120, and 124 hours, the increase in the number of cells was compared. . The control water culture solution is a culture solution in which the culture solution powder {Eagle MEM medium powder (Minimum Essential Medium)} is dissolved in the Millipore water produced from tap water and then sterilized by autoclaving. Is a culture solution in which the same culture solution powder is dissolved in the modified water obtained in Example 4 and then sterilized by autoclaving.
The number of cells was calculated using a hemocytometer. In order to avoid the cells from weakening and dying, the control water culture solution and the modified water culture solution were replaced with new ones 72 hours after the start of the culture.
FIG. 5 is a graph showing changes over time in the number of cells cultured in the modified water culture solution and the control water culture solution.
As is clear from FIG. 5, the number of fibroblasts of Examples cultured in the modified water culture solution was significantly higher than the number of fibroblasts cultured in the control water culture solution of Comparative Example from 72 hours after the start of the culture. (P <0.01), it was shown that the modified water has a cell growth promoting action.
As described above, according to the present example, it was revealed that the modified water has an excellent cell growth promoting action. From this, it was found that the modified water can be expected to promote wound healing and improve the living environment of cells.
（実施例６）
（活力増強剤としての評価）
インキュベータ内（蛍光灯20W３本を使用、室温25℃）に恒温槽（型式：T−22L）を設置して、その中の養液栽培装置（縦53cm×横36cm×高さ19cm）の容器上に定植パネル（定植孔直径2.5mm、孔間隔2.0cm ×3.5cm、孔数40）を置き、送水ポンプ（流量１０Ｌ／分）で培養液を循環供給させる方式により、コマツナ（夏楽天・タキイ交配）苗の根による吸収比較を行った。苗は水道水で生育させたものを用い、培養液は水道水にみかど水耕１号、２号を配合して初期ｐＨを６．０、導電率を３．０ｄＳ／ｍに調整した原料水を、実施例１で得られたセラミック粒子と容積１Ｌのポリプロピレン製の容器に入れ、毎秒１回上下に往復する振とう機を使って５分間振とうし、セラミック粒子を流動・衝突させて改質処理したものを用いた。なお、振とう機の容器を振とうさせる加速度は、セラミック粒子が水中で０．０３〜０．２ｍ／ｓの速度で流動するように設定した。以上の改質処理を行った処理培養液と未処理培養液（対照区）の２試験区を設けた。各培養液は１週間毎に新しいものと交換した。
栽培野菜の品質に関わる実験として、収穫後のコマツナの鮮度保持比較実験を行った。収穫後のコマツナを新聞紙1枚に包んだ状態で５℃に維持された環境制御室内に保存し、経過時間ごとに重量を測定し、水分減少率（％）＝（初期重量−経過時間後の重量）÷初期重量×100を求めた。
表１は、秋期に収穫したコマツナによる水分減少率を、収穫後の経過時間毎に２４時間まで比較した結果である。表中の値は、対照区、処理区とも５株×６回反復（計３０株）における水分減少率（％）の平均値であり、カッコ内は標準偏差である。
(Example 6)
(Evaluation as vitality enhancer)
A thermostatic bath (model: T-22L) is installed in the incubator (using three 20W fluorescent lamps,
As an experiment related to the quality of cultivated vegetables, we conducted a comparative experiment on maintaining the freshness of Komatsuna after harvest. The harvested Komatsuna is wrapped in a piece of newspaper and stored in an environmental control room maintained at 5 ° C. The weight is measured at each elapsed time, and the water loss rate (%) = (initial weight-after the elapsed time) Weight) ÷ initial weight × 100.
Table 1 shows the results of comparing the water reduction rate by Komatsuna harvested in the fall to 24 hours for each elapsed time after harvesting. The values in the table are the average value of the water reduction rate (%) in 5 strains × 6 repetitions (total 30 strains) in both the control group and the treated group, and the parenthesized values are standard deviations.
これより、対照区に対して、処理区の水分減少率は常に小さく維持されており、対照区に比べ、処理区のコマツナは収穫後の水分を保持する能力は高いといえる。
よって、本実施例によれば、改質水で育成された植物は収穫後の鮮度保持力が高く、本改質水は活力増強剤としても優れていることが明らかである。
Thus, the water loss rate of the treated group is always kept small compared to the control group, and it can be said that the Komatsuna of the treated group has a higher ability to retain the water after harvesting than the control group.
Therefore, according to the present Example, it is clear that the plant grown with the reformed water has a high freshness retention power after harvesting, and the reformed water is also excellent as a vitality enhancer.
（実施例７）
原料水として、宮崎県宮崎市の水道水を使用し、実施例１で得られたセラミック粒子を用いて改質水を以下の方法により製造した。
５００ｍｌのポリエチレンテレフタレート製の蓋付きの容器を用意し、該セラミック粒子を容器の内容積２５％となるように充填した。該容器に原料水である宮崎市の水道水を内容積６０％となるよう導入した後、蓋を閉め、該容器をセラミック粒子が水中で流動、相互摩擦、衝突するよう数回振って改質水を得た。なお、改質水の１Ｈ−核磁気共鳴スペクトルは図２（ａ）と同様のスペクトルであり、原料水の１Ｈ−核磁気共鳴スペクトルは図２（ｂ）と同様のスペクトルであった。また、パルス核磁気共鳴装置（ＪＮＭ−ＭＵ２５Ａ）（ＣＰＭＧ法）を用いて改質水と原料水の１Ｈ−パルス核磁気共鳴のスピン−スピン緩和時間を測定し、その緩和時間から求めた短縮率は８％であった。
１日に体内に摂取する水を原料水あるいは改質水のみとする条件で、便秘の２０歳代〜５０歳代の女性１０名を対象に、原料水を1日に２．５Ｌずつ連続して１週間飲料してもらい、その後連続して１週間は該改質水を１日に２．５Ｌずつ飲料してもらうこととし、改質水が便秘に与える影響を調査した。その結果、摂取飲料水が改質水の場合に便秘が治ったあるいは回数が減少し、便秘解消・低減に効果が認められたと答えた女性は１０名中８名であり、実験対象者全体の８０％に該改質水の便秘解消・低減効果が認められた。
本実施例によれば、改質水は便秘の解消や低減にも効果があることが確認された。これは、改質水が胃腸内の異常発酵を抑制したからであると推察される。
(Example 7)
As raw material water, tap water from Miyazaki City, Miyazaki Prefecture was used, and the modified water was produced by the following method using the ceramic particles obtained in Example 1.
A container with a lid made of 500 ml of polyethylene terephthalate was prepared, and the ceramic particles were filled so that the inner volume of the container was 25%. After introducing Miyazaki City tap water, which is the raw material water, into the container so as to have an internal volume of 60%, the lid is closed and the container is shaken several times so that the ceramic particles flow, mutually friction and collide in the water. Got water. The 1 H-nuclear magnetic resonance spectrum of the reformed water was the same as that shown in FIG. 2A, and the 1 H-nuclear magnetic resonance spectrum of the raw water was the same spectrum as that shown in FIG. In addition, the spin-spin relaxation time of 1 H-pulse nuclear magnetic resonance of reformed water and raw water was measured using a pulsed nuclear magnetic resonance apparatus (JNM-MU25A) (CPMG method), and the shortening obtained from the relaxation time. The rate was 8%.
Continuing with 2.5 liters of raw water per day for 10 women in their 20s to 50s who are constipated under the condition that the water consumed in the body per day is only raw water or modified water. Drink for 1 week and then drink 2.5 L per day of the reformed water for 1 week continuously, and investigate the effect of the reformed water on constipation. As a result, 8 out of 10 women answered that constipation was cured or reduced in number when drinking water was reformed water, and the effect was confirmed in the elimination and reduction of constipation. 80% of the reformed water was found to have an effect of eliminating and reducing constipation.
According to this example, it was confirmed that the reformed water is effective in eliminating or reducing constipation. This is presumably because the modified water suppressed abnormal fermentation in the gastrointestinal tract.
（実施例８）
実施例１で得たセラミック粒子３ｇをガラスアトマイザー（３０ｍｌ容量スプレー容器、株式会社マコト製）に入れ、香川県さぬき市志度の水道水を原料水として注入し、セラミック粒子が流動し互いに衝突するように、１０数回上下に振って改質水を得た。なお、改質水の１Ｈ−核磁気共鳴スペクトルは図２（ａ）と同様のスペクトルであり、原料水の１Ｈ−核磁気共鳴スペクトルは図２（ｂ）と同様のスペクトルであった。また、パルス核磁気共鳴装置（ＪＮＭ−ＭＵ２５Ａ）（ＣＰＭＧ法）を用いて改質水と原料水の１Ｈ−パルス核磁気共鳴のスピン−スピン緩和時間を測定し、その緩和時間から求めた短縮率は１２％であった。
この改質水を荒れ性肌の人に噴霧しパッチングしたところ、長時間に亘って滑らかな肌を維持した。また、蚊に刺された部位に噴霧すると、如実にかゆみ止め効果を確認した。さらに、充血した眼球に噴霧すると、速やかに充血を抑制する効果を認めた。
(Example 8)
3 g of the ceramic particles obtained in Example 1 are put into a glass atomizer (30 ml capacity spray container, manufactured by Makoto Co., Ltd.), and tap water of Sanuki City, Kagawa Prefecture is injected as raw water so that the ceramic particles flow and collide with each other. Then, it was shaken up and down 10 times to obtain reformed water. The 1 H-nuclear magnetic resonance spectrum of the reformed water was the same as that shown in FIG. 2A, and the 1 H-nuclear magnetic resonance spectrum of the raw water was the same spectrum as that shown in FIG. In addition, the spin-spin relaxation time of 1 H-pulse nuclear magnetic resonance of reformed water and raw water was measured using a pulsed nuclear magnetic resonance apparatus (JNM-MU25A) (CPMG method), and the shortening obtained from the relaxation time. The rate was 12%.
When this modified water was sprayed and patched on a person with rough skin, smooth skin was maintained for a long time. Moreover, when it sprayed on the site | part stabbed by the mosquito, it confirmed the anti-itching effect indeed. Furthermore, when sprayed on the congested eyeball, an effect of quickly suppressing congestion was recognized.
（実施例９）
実施例１で得たセラミック粒子２０ｇを内容量２Lのポリエチレンテレフタレート製の蓋付きの容器に入れ、宮崎県宮崎市の対象者（男性２０名）の家庭に配布した。水道水を原料水として対象者の家庭で注いでもらい、セラミック粒子が流動・衝突するように、容器を上下逆さまにする動作を１０回実施し、原料水を改質処理し改質水を製造してもらった。なお、改質水の１Ｈ−核磁気共鳴スペクトルは図２（ａ）と同様のスペクトルであり、原料水の１Ｈ−核磁気共鳴スペクトルは図２（ｂ）と同様のスペクトルであった。また、パルス核磁気共鳴装置（ＪＮＭ−ＭＵ２５Ａ）（ＣＰＭＧ法）を用いて改質水と原料水の１Ｈ−パルス核磁気共鳴のスピン−スピン緩和時間を測定し、その緩和時間から求めた短縮率は１０％であった。
対象者には、この改質水を使って頭髪を1回／日の頻度で洗浄してもらった。１カ月続けたところ、対象者の６０％以上が発毛・増毛の効果を認めた。
Example 9
20 g of the ceramic particles obtained in Example 1 were put in a container with a lid made of polyethylene terephthalate having an internal volume of 2 L and distributed to the homes of subjects (20 men) in Miyazaki City, Miyazaki Prefecture. Tap water is poured into the subject's home as raw water, and the container is turned upside down 10 times so that the ceramic particles flow and collide, and the raw water is reformed to produce reformed water. I was asked to. The 1 H-nuclear magnetic resonance spectrum of the reformed water was the same as that shown in FIG. 2A, and the 1 H-nuclear magnetic resonance spectrum of the raw water was the same spectrum as that shown in FIG. In addition, the spin-spin relaxation time of 1 H-pulse nuclear magnetic resonance of reformed water and raw water was measured using a pulsed nuclear magnetic resonance apparatus (JNM-MU25A) (CPMG method), and the shortening obtained from the relaxation time. The rate was 10%.
The subject used this modified water to clean their hair once a day. When continued for one month, more than 60% of the subjects recognized the effects of hair growth and hair growth.
本発明は、抗酸化作用を有する改質水及びそれを含有する抗酸化ストレス剤、細胞増殖促進剤並びに活力増強剤に関し、水の構造を変化させて抗酸化性を付与しているので、改質水を摂取した人体が負担を受けることなく、線維芽細胞等の動物由来の細胞に対する酸化ストレスの抑制、線維芽細胞等の動物由来の細胞に対する増殖促進作用、植物や魚介類等の鮮度の保持や活力増強、動物の整腸効果といった特異的な効果を得ることができ、植物鮮度保持効果、動物の整腸効果、発毛・増毛効果、線維芽細胞に対する酸化ストレスの抑制のみならず、酸化ストレスが関与する疾患、即ち、体外からの作用としては皮膚の老化予防、アトピー性皮膚炎の改善、皮膚のメラニン色素沈着の防止、改善などに、動脈硬化、肺気腫、肺繊維症、喘息、糖尿病、すべての臓器の虚血再環流障害、白内障及び発癌の抑制、あるいは、高圧酸素療法における副作用の防止などに効果があると考えられる改質水を提供することができ、また改質水を主成分とした抗酸化ストレス剤、細胞増殖促進剤並びに活力増強剤を提供することができ、その応用は、記載用途のみに限定されるものではなく、医療、保健・健康、薬、衛生、農林水産業、工業、畜産業など多岐に渡る。 The present invention relates to a modified water having an antioxidant action and an antioxidant stress agent, a cell growth promoter and a vitality enhancer containing the same, because the structure of water is changed to impart antioxidant properties. The human body that ingested quality water is not burdened, suppresses oxidative stress on cells derived from animals such as fibroblasts, promotes the growth of cells derived from animals such as fibroblasts, and the freshness of plants, seafood, etc. Specific effects such as retention and vitality enhancement, animal intestinal effect, plant freshness retention effect, animal intestinal effect, hair growth / hair growth effect, suppression of oxidative stress on fibroblasts, Diseases involving oxidative stress, i.e., external effects include skin aging prevention, improvement of atopic dermatitis, prevention of skin melanin pigmentation, improvement, etc., arteriosclerosis, emphysema, pulmonary fibrosis, asthma, It is possible to provide modified water that is considered to be effective in suppressing urinary disease, ischemia / reperfusion injury of all organs, cataract and carcinogenesis, or preventing side effects in hyperbaric oxygen therapy. Can be provided as antioxidant stress agent, cell growth promoter and vitality enhancer, and its application is not limited only to the described use, but also medical, health / health, medicine, hygiene, A wide range of industries including agriculture, forestry and fisheries, industry, and livestock.
Claims (5)
重クロロホルム中、内部基準にテトラメチルシランを使用して測定した１Ｈ−核磁気共鳴スペクトル（４００ＭＨｚ）が、次の（ａ）乃至（ｄ）の内いずれか１以上であることを特徴とする改質水。
（ａ）主吸収ピークより低磁場側に、前記原料水には存在しなかった吸収ピークが出現したスペクトル
（ｂ）前記原料水の主吸収ピークより高磁場側に存在した少なくとも一つの吸収ピークが消失したスペクトル
（ｃ）前記原料水の主吸収ピークより高磁場側に存在した少なくとも一つの吸収ピークが低磁場側にシフトしたスペクトル
（ｄ）主吸収ピークの半値幅が、前記原料水の主吸収ピークの半値幅より増大したスペクトル Reformed water that has been reformed by applying external stimulus to the raw material water,
1 H-nuclear magnetic resonance spectrum (400 MHz) measured using tetramethylsilane as an internal standard in deuterated chloroform is one or more of the following (a) to (d): Reformed water.
(A) Spectrum in which an absorption peak that did not exist in the raw material water appears on the lower magnetic field side than the main absorption peak (b) At least one absorption peak present on the higher magnetic field side than the main absorption peak of the raw material water Disappeared spectrum (c) Spectrum in which at least one absorption peak present on the higher magnetic field side than the main absorption peak of the raw material water is shifted to the lower magnetic field side (d) The half width of the main absorption peak is the main absorption of the raw water Spectrum increased beyond the half-width of the peak
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